MD12G1118100.v1.1

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016322
Physical Location & Seq
Forward (+)
18960890 .. 18962605
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1118100.v1.1.491

Sequence Viewer

Length: 723 bp
ATGATTACGTTTATGGATTCAAAAGAGAAACTCAAGGAGGGGGATAGGTGCTTAGATCCTCAGCTATGGCATGCCTGCGCTGGCGGCATGGTTCAAATGCCTCCGGTGAATGCCAAGGTCTTCTACTTCCCTCAGGGCCATGCTGAGCATGCCTGTGGACCTGTTGATTTCAGGAACTGCCCAAGAGTTCCTGCTCATATACTTTGCCGGGTCGCCGCCATTAAGTTCATGGCTGATCCTGGGACTGATGAGGTTTATGCAAAAATTAGGTTGGTTCCTTTGAATGGGGCCGAGGCAGGTTACGAGGATGATGGAATTGGAGGGCTTAATGGGACTGAAACGCCGGATAAACCTGCCTCTTTTGCAAAGACGTTGACACAATCCGATGCAAACAATGGCGGTGGGTTCTCTGTTCCGAGATATTGTGCAGAAACCATCTTTCCGCGATTGGATTACTCTGCCGATCCCCCTGTTCAGACAATCCTTGCTAAGGATGTCCATGGGGAAACATGGAAGTTCAGGCACATTTACAGAGGGACCCCTAGGCGGCATCTATTGACCACAGGGTGGAGTACCTTTGTGAACCACAAGAAGCTTGTTTCTGGGGACTCAATTGTGTTTTTGAGGGCAGAGAATGGTGATCTCTGTGTTGGAATTCGTCGCGCAAAGAGGGGAATTGGAGGTGGATCAGAGTCATCTTCAGGGTGGAATCCAATGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_resp B3

Protein Analysis

241

Amino Acids

26.19

Weight (kDa)

8.15

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 121 - 222 1.2e-23 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 287, 361
AccB7I CCANNNNNTGG 1 cut(s) 567
AccII CGCG 2 cut(s) 445, 663
AciI CCGC 5 cut(s) 84, 216, 399, 443, 547
AclWI GGATC 4 cut(s) 50, 230, 458, 694
AcsI RAATTY 1 cut(s) 654
AcuI CTGAAG 1 cut(s) 684
AdeI CACNNNGTG 1 cut(s) 567
AfaI GTAC 1 cut(s) 574
AfiI CCNNNNNNNGG 4 cut(s) 284, 490, 546, 567
AgsI TTSAA 3 cut(s) 21, 95, 283
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 2 cut(s) 64, 595
AluI AGCT 2 cut(s) 64, 595
AlwI GGATC 4 cut(s) 50, 230, 458, 694
AlwNI CAGNNNCTG 1 cut(s) 177
AoxI GGCC 2 cut(s) 136, 288
ApoI RAATTY 1 cut(s) 654
AspA2I CCTAGG 1 cut(s) 542
AspLEI GCGC 2 cut(s) 80, 665
AspS9I GGNCC 4 cut(s) 136, 158, 288, 537
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 2 cut(s) 118, 650
AvaII GGWCC 2 cut(s) 158, 537
AvrII CCTAGG 1 cut(s) 542
AxyI CCTNAGG 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 112
BbvCI CCTCAGC 1 cut(s) 60
BccI CCATC 2 cut(s) 305, 443
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 1 cut(s) 209
BfaI CTAG 1 cut(s) 543
BfuAI ACCTGC 2 cut(s) 287, 361
BisI GCNGC 3 cut(s) 85, 216, 548
BlnI CCTAGG 1 cut(s) 542
BlpI GCTNAGC 1 cut(s) 144
BlsI GCNGC 3 cut(s) 86, 217, 549
Bme1390I CCNGG 2 cut(s) 209, 240
Bme18I GGWCC 2 cut(s) 158, 537
BmgT120I GGNCC 4 cut(s) 136, 158, 288, 537
BmiI GGNNCC 4 cut(s) 276, 289, 538, 539
BmrFI CCNGG 2 cut(s) 209, 240
BmsI GCATC 2 cut(s) 376, 559
BpiI GAAGAC 1 cut(s) 112
Bpu10I CCTNAGC 2 cut(s) 60, 489
Bpu1102I GCTNAGC 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 209
BsaJI CCNNGG 5 cut(s) 114, 239, 291, 499, 542
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 490, 546, 567
Bse21I CCTNAGG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 5 cut(s) 114, 239, 291, 499, 542
BseGI GGATG 2 cut(s) 313, 499
BseLI CCNNNNNNNGG 4 cut(s) 284, 490, 546, 567
BseMII CTCAG 3 cut(s) 74, 135, 146
BsgI GTGCAG 1 cut(s) 447
Bsh1236I CGCG 2 cut(s) 445, 663
BshFI GGCC 2 cut(s) 138, 290
BsiSI CCGG 3 cut(s) 104, 208, 344
BslFI GGGAC 4 cut(s) 256, 346, 550, 620
BslI CCNNNNNNNGG 4 cut(s) 284, 490, 546, 567
BsmFI GGGAC 4 cut(s) 256, 346, 550, 620
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 2 cut(s) 138, 290
Bsp143I GATC 5 cut(s) 55, 235, 463, 640, 686
Bsp1720I GCTNAGC 1 cut(s) 144
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 5 cut(s) 84, 216, 399, 443, 547
BspANI GGCC 2 cut(s) 138, 290
BspCNI CTCAG 3 cut(s) 73, 136, 145
BspFNI CGCG 2 cut(s) 445, 663
BspLI GGNNCC 4 cut(s) 276, 289, 538, 539
BspMI ACCTGC 2 cut(s) 287, 361
BspPI GGATC 4 cut(s) 50, 230, 458, 694
BssECI CCNNGG 5 cut(s) 114, 239, 291, 499, 542
BssMI GATC 5 cut(s) 55, 235, 463, 640, 686
BssT1I CCWWGG 3 cut(s) 114, 499, 542
Bst2UI CCWGG 1 cut(s) 240
BstC8I GCNNGC 4 cut(s) 72, 76, 82, 150
BstDEI CTNAG 5 cut(s) 52, 60, 132, 144, 489
BstDSI CCRYGG 1 cut(s) 499
BstENI CCTNNNNNAGG 1 cut(s) 488
BstF5I GGATG 2 cut(s) 313, 499
BstFNI CGCG 2 cut(s) 445, 663
BstHHI GCGC 2 cut(s) 80, 665
BstKTI GATC 5 cut(s) 58, 238, 466, 643, 689
BstMBI GATC 5 cut(s) 55, 235, 463, 640, 686
BstMWI GCNNNNNNNGC 3 cut(s) 84, 149, 362
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 2 cut(s) 74, 152
BstSCI CCNGG 2 cut(s) 207, 238
BstUI CGCG 2 cut(s) 445, 663
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
Bsu36I CCTNAGG 1 cut(s) 132
BsuRI GGCC 2 cut(s) 138, 290
BtgI CCRYGG 1 cut(s) 499
BtsCI GGATG 2 cut(s) 313, 499
BveI ACCTGC 2 cut(s) 287, 361
Cac8I GCNNGC 4 cut(s) 72, 76, 82, 150
CaiI CAGNNNCTG 1 cut(s) 177
CfoI GCGC 2 cut(s) 80, 665
Cfr13I GGNCC 4 cut(s) 136, 158, 288, 537
Csp6I GTAC 1 cut(s) 573
CspCI CAANNNNNGTGG 2 cut(s) 382, 417
CviAII CATG 7 cut(s) 71, 88, 140, 149, 229, 500, 510
CviJI RGCY 6 cut(s) 64, 138, 233, 290, 325, 595
CviKI_1 RGCY 6 cut(s) 64, 138, 233, 290, 325, 595
CviQI GTAC 1 cut(s) 573
DdeI CTNAG 5 cut(s) 52, 60, 132, 144, 489
DpnI GATC 5 cut(s) 57, 237, 465, 642, 688
DpnII GATC 5 cut(s) 55, 235, 463, 640, 686
DraIII CACNNNGTG 1 cut(s) 567
Eco130I CCWWGG 3 cut(s) 114, 499, 542
Eco47I GGWCC 2 cut(s) 158, 537
Eco57I CTGAAG 1 cut(s) 684
Eco81I CCTNAGG 1 cut(s) 132
EcoNI CCTNNNNNAGG 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 537
EcoRI GAATTC 1 cut(s) 654
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 3 cut(s) 114, 499, 542
ErhI CCWWGG 3 cut(s) 114, 499, 542
FaeI CATG 7 cut(s) 74, 91, 143, 152, 232, 503, 513
FaqI GGGAC 4 cut(s) 256, 346, 550, 620
FatI CATG 7 cut(s) 70, 87, 139, 148, 228, 499, 509
Fnu4HI GCNGC 3 cut(s) 85, 216, 548
FokI GGATG 2 cut(s) 320, 506
Fsp4HI GCNGC 3 cut(s) 85, 216, 548
FspBI CTAG 1 cut(s) 543
GlaI GCGC 2 cut(s) 79, 664
GluI GCNGC 3 cut(s) 85, 216, 548
HaeIII GGCC 2 cut(s) 138, 290
HapII CCGG 3 cut(s) 104, 208, 344
HhaI GCGC 2 cut(s) 80, 665
Hin1II CATG 7 cut(s) 74, 91, 143, 152, 232, 503, 513
Hin6I GCGC 2 cut(s) 78, 663
HinP1I GCGC 2 cut(s) 78, 663
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 4 cut(s) 17, 608, 692, 709
HpaII CCGG 3 cut(s) 104, 208, 344
HphI GGTGA 2 cut(s) 118, 650
Hpy166II GTNNAC 3 cut(s) 158, 375, 583
Hpy188I TCNGA 4 cut(s) 385, 417, 477, 691
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 3 cut(s) 158, 375, 583
Hpy99I CGWCG 1 cut(s) 663
HpyCH4IV ACGT 2 cut(s) 8, 371
HpyCH4V TGCA 4 cut(s) 260, 365, 389, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 149, 362
HpyF3I CTNAG 5 cut(s) 52, 60, 132, 144, 489
HpySE526I ACGT 2 cut(s) 8, 371
Hsp92II CATG 7 cut(s) 74, 91, 143, 152, 232, 503, 513
HspAI GCGC 2 cut(s) 78, 663
KflI GGGWCCC 1 cut(s) 537
Kzo9I GATC 5 cut(s) 55, 235, 463, 640, 686
LweI GCATC 2 cut(s) 376, 559
MaeI CTAG 1 cut(s) 543
MaeII ACGT 2 cut(s) 8, 371
MaeIII GTNAC 1 cut(s) 299
MalI GATC 5 cut(s) 57, 237, 465, 642, 688
MboI GATC 5 cut(s) 55, 235, 463, 640, 686
MboII GAAGA 2 cut(s) 112, 690
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 55
MluCI AATT 5 cut(s) 264, 315, 612, 654, 675
MlyI GAGTC 2 cut(s) 602, 701
MmeI TCCRAC 1 cut(s) 631
MseI TTAA 2 cut(s) 222, 327
MslI CAYNNNNRTG 1 cut(s) 153
MspI CCGG 3 cut(s) 104, 208, 344
MspR9I CCNGG 2 cut(s) 209, 240
MunI CAATTG 1 cut(s) 612
Mva1269I GAATGC 1 cut(s) 115
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 2 cut(s) 445, 663
MwoI GCNNNNNNNGC 3 cut(s) 84, 149, 362
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 499
NdeII GATC 5 cut(s) 55, 235, 463, 640, 686
NlaIII CATG 7 cut(s) 74, 91, 143, 152, 232, 503, 513
NlaIV GGNNCC 4 cut(s) 276, 289, 538, 539
NmeAIII GCCGAG 1 cut(s) 316
NspI RCATGY 2 cut(s) 74, 152
PaeI GCATGC 2 cut(s) 74, 152
PctI GAATGC 1 cut(s) 115
PfeI GAWTC 2 cut(s) 17, 709
PflMI CCANNNNNTGG 1 cut(s) 567
PkrI GCNGC 3 cut(s) 86, 217, 549
PleI GAGTC 2 cut(s) 602, 700
PpsI GAGTC 2 cut(s) 602, 700
PpuMI RGGWCCY 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 4 cut(s) 276, 289, 538, 539
PspPI GGNCC 4 cut(s) 136, 158, 288, 537
PspPPI RGGWCCY 1 cut(s) 537
PstNI CAGNNNCTG 1 cut(s) 177
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 1 cut(s) 574
RsaNI GTAC 1 cut(s) 573
RseI CAYNNNNRTG 1 cut(s) 153
SaqAI TTAA 2 cut(s) 222, 327
SatI GCNGC 3 cut(s) 85, 216, 548
Sau3AI GATC 5 cut(s) 55, 235, 463, 640, 686
Sau96I GGNCC 4 cut(s) 136, 158, 288, 537
SchI GAGTC 2 cut(s) 602, 701
ScrFI CCNGG 2 cut(s) 209, 240
SfaNI GCATC 2 cut(s) 376, 559
SinI GGWCC 2 cut(s) 158, 537
SmiMI CAYNNNNRTG 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SphI GCATGC 2 cut(s) 74, 152
Sse9I AATT 5 cut(s) 264, 315, 612, 654, 675
SsiI CCGC 5 cut(s) 84, 216, 399, 443, 547
SspMI CTAG 1 cut(s) 543
StyD4I CCNGG 2 cut(s) 207, 238
StyI CCWWGG 3 cut(s) 114, 499, 542
TaiI ACGT 2 cut(s) 11, 374
TasI AATT 5 cut(s) 264, 315, 612, 654, 675
TauI GCSGC 3 cut(s) 87, 218, 550
TfiI GAWTC 2 cut(s) 17, 709
Tru1I TTAA 2 cut(s) 222, 327
Tru9I TTAA 2 cut(s) 222, 327
TspDTI ATGAA 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 567
VpaK11BI GGWCC 2 cut(s) 158, 537
XagI CCTNNNNNAGG 1 cut(s) 488
XapI RAATTY 1 cut(s) 654
XceI RCATGY 2 cut(s) 74, 152
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XmaJI CCTAGG 1 cut(s) 542
XspI CTAG 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.