Rmu_sc0032469.1_g000003

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032469.1
OG0016322
Physical Location & Seq
Forward (+)
7019 .. 11197
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032469.1_g000003.1.cds

Sequence Viewer

Length: 1095 bp
atgcaagaagagaagccgcgcttccttgtgggacttgcggccatagacgcaacatcactatcaatatggatactggcaccatctcctagtgcctcaagagcggcgttacgtccagtatttgggacattaattcttgcaggcacaatttgtaatagttatatgagatctcgtcagtttagcaacatcagaaaatgcatcaggcagagtatatgcgatgttttggagctcgattattcttcacacttcgagttcggactgtgcgggctgtgggaagtggcatccacgacaccatggccatgggcattgacatccatggcatcatggccatggctgacgccatctatggctttttcaaggggggatagtggtggccctaggccaacggtggatggcgtgctgaccaagtttgccgccaccgcctccgacctcaaagccattgaccacgcctcttccttcctgttctcgccggattccgatacccatttatacgatgcatttttcttcaacaccatcatcagggcttacgctcagactggtcatgcgaagcataaagctttgcgcttttacaaagttatggttaaaggtgaagtcttgcctaataaattcacctacccttttgttctcaaggcctatgccggcgtcggggacttgaatttggggaagccggttcatgggtcggtggtgaaattcgggtttggtgatgatgtccatgttcggaacaccatggttcatatgtactgttgctgcagtggagggattgaaagtgcacgaaaggtgtttgatgaaatgcctaactgggactctgtttcgtggagcgcgatgattggagggtatgtgagggtggggtggtctagtgatgcggtggagttgtttaaggagatgcagatggcgggagtgaggacggatgagattactatggcttcggtttttttgtggtggcaatcgagcatgaaacgagccatggacgatgcaacaagggtggaaagaagtggtgatgctacttcggaggtcactcatagaacttctgatttcggccacctcaggcgaagtttcttgagggcttttgaagctgagggcatagaagctttcttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

364

Amino Acids

40.54

Weight (kDa)

7.14

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AccB7I CCANNNNNTGG 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 101
AccII CGCG 2 cut(s) 19, 818
AciI CCGC 8 cut(s) 17, 38, 101, 261, 411, 417, 860, 890
AcoI YGGCCR 4 cut(s) 39, 293, 323, 1033
AcsI RAATTY 3 cut(s) 602, 652, 686
AcyI GRCGYC 2 cut(s) 335, 639
AfaI GTAC 1 cut(s) 737
AfiI CCNNNNNNNGG 4 cut(s) 119, 516, 642, 671
AgsI TTSAA 5 cut(s) 354, 505, 652, 761, 1067
AluBI AGCT 4 cut(s) 226, 554, 1070, 1085
AluI AGCT 4 cut(s) 226, 554, 1070, 1085
Alw21I GWGCWC 2 cut(s) 228, 769
Alw44I GTGCAC 1 cut(s) 765
AoxI GGCC 7 cut(s) 39, 293, 323, 370, 377, 627, 1033
ApaLI GTGCAC 1 cut(s) 765
ApeKI GCWGC 1 cut(s) 744
ApoI RAATTY 3 cut(s) 602, 652, 686
AseI ATTAAT 1 cut(s) 128
AspA2I CCTAGG 1 cut(s) 374
AspLEI GCGC 3 cut(s) 21, 561, 818
AspS9I GGNCC 1 cut(s) 371
AsuHPI GGTGA 5 cut(s) 596, 598, 694, 710, 1004
AvrII CCTAGG 1 cut(s) 374
AxyI CCTNAGG 1 cut(s) 1040
BaeGI GKGCMC 1 cut(s) 769
BalI TGGCCA 2 cut(s) 295, 325
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 228
Bbv12I GWGCWC 2 cut(s) 228, 769
BbvCI CCTCAGC 1 cut(s) 1071
BbvI GCAGC 1 cut(s) 731
BccI CCATC 5 cut(s) 88, 346, 383, 518, 880
BciVI GTATCC 1 cut(s) 63
BfaI CTAG 3 cut(s) 87, 375, 852
BfmI CTRYAG 1 cut(s) 745
BfuI GTATCC 1 cut(s) 63
BglII AGATCT 1 cut(s) 164
BisI GCNGC 5 cut(s) 17, 39, 102, 411, 745
BlnI CCTAGG 1 cut(s) 374
BlsI GCNGC 5 cut(s) 18, 40, 103, 412, 746
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 1 cut(s) 78
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 8 cut(s) 204, 287, 326, 481, 847, 870, 958, 985
BmuI ACTGGG 1 cut(s) 805
Bpu10I CCTNAGC 1 cut(s) 1071
BpuEI CTTGAG 3 cut(s) 79, 608, 1075
BsaHI GRCGYC 2 cut(s) 335, 639
BsaJI CCNNGG 7 cut(s) 290, 296, 312, 326, 374, 723, 960
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 516, 642, 671
Bse118I RCCGGY 2 cut(s) 635, 664
Bse1I ACTGG 4 cut(s) 78, 113, 538, 800
Bse21I CCTNAGG 1 cut(s) 1040
BseDI CCNNGG 7 cut(s) 290, 296, 312, 326, 374, 723, 960
BseGI GGATG 4 cut(s) 278, 308, 394, 910
BseLI CCNNNNNNNGG 4 cut(s) 119, 516, 642, 671
BseMII CTCAG 3 cut(s) 542, 1054, 1062
BseNI ACTGG 4 cut(s) 78, 113, 538, 800
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 1 cut(s) 731
Bsh1236I CGCG 2 cut(s) 19, 818
BshFI GGCC 7 cut(s) 41, 295, 325, 372, 379, 629, 1035
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 2 cut(s) 228, 769
BsiSI CCGG 3 cut(s) 467, 636, 665
BslFI GGGAC 4 cut(s) 45, 136, 659, 812
BslI CCNNNNNNNGG 4 cut(s) 119, 516, 642, 671
BsmFI GGGAC 4 cut(s) 45, 136, 659, 812
BsnI GGCC 7 cut(s) 41, 295, 325, 372, 379, 629, 1035
Bsp1286I GDGCHC 2 cut(s) 228, 769
Bsp143I GATC 1 cut(s) 164
Bsp19I CCATGG 6 cut(s) 290, 296, 312, 326, 723, 960
BspACI CCGC 8 cut(s) 17, 38, 101, 261, 411, 417, 860, 890
BspANI GGCC 7 cut(s) 41, 295, 325, 372, 379, 629, 1035
BspCNI CTCAG 3 cut(s) 541, 1053, 1063
BspFNI CGCG 2 cut(s) 19, 818
BspLI GGNNCC 1 cut(s) 78
BspMAI CTGCAG 1 cut(s) 749
BspT107I GGYRCC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 101
BsrFI RCCGGY 2 cut(s) 635, 664
BsrI ACTGG 4 cut(s) 78, 113, 538, 800
BssAI RCCGGY 2 cut(s) 635, 664
BssECI CCNNGG 7 cut(s) 290, 296, 312, 326, 374, 723, 960
BssMI GATC 1 cut(s) 164
BssNI GRCGYC 2 cut(s) 335, 639
BssT1I CCWWGG 7 cut(s) 290, 296, 312, 326, 374, 723, 960
Bst4CI ACNGT 3 cut(s) 258, 385, 740
Bst6I CTCTTC 2 cut(s) 3, 454
BstACI GRCGYC 2 cut(s) 335, 639
BstC8I GCNNGC 4 cut(s) 139, 263, 395, 637
BstDEI CTNAG 3 cut(s) 528, 1040, 1071
BstDSI CCRYGG 6 cut(s) 290, 296, 312, 326, 723, 960
BstF5I GGATG 4 cut(s) 278, 308, 394, 910
BstFNI CGCG 2 cut(s) 19, 818
BstHHI GCGC 3 cut(s) 21, 561, 818
BstKTI GATC 1 cut(s) 167
BstMBI GATC 1 cut(s) 164
BstMWI GCNNNNNNNGC 4 cut(s) 47, 98, 416, 1067
BstSFI CTRYAG 1 cut(s) 745
BstSLI GKGCMC 1 cut(s) 769
BstUI CGCG 2 cut(s) 19, 818
BstV1I GCAGC 1 cut(s) 731
BstX2I RGATCY 1 cut(s) 164
BstXI CCANNNNNNTGG 1 cut(s) 297
BstYI RGATCY 1 cut(s) 164
Bsu36I CCTNAGG 1 cut(s) 1040
BsuI GTATCC 1 cut(s) 63
BsuRI GGCC 7 cut(s) 41, 295, 325, 372, 379, 629, 1035
BtgI CCRYGG 6 cut(s) 290, 296, 312, 326, 723, 960
BtgZI GCGATG 2 cut(s) 228, 833
BtsCI GGATG 4 cut(s) 278, 308, 394, 910
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 1 cut(s) 754
Cac8I GCNNGC 4 cut(s) 139, 263, 395, 637
CfoI GCGC 3 cut(s) 21, 561, 818
Cfr10I RCCGGY 2 cut(s) 635, 664
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 3 cut(s) 56, 343, 628
Csp6I GTAC 1 cut(s) 736
CspCI CAANNNNNGTGG 2 cut(s) 960, 995
CviQI GTAC 1 cut(s) 736
DdeI CTNAG 3 cut(s) 528, 1040, 1071
DpnI GATC 1 cut(s) 166
DpnII GATC 1 cut(s) 164
EaeI YGGCCR 4 cut(s) 39, 293, 323, 1033
Eam1104I CTCTTC 2 cut(s) 3, 454
EarI CTCTTC 2 cut(s) 3, 454
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 7 cut(s) 290, 296, 312, 326, 374, 723, 960
Eco147I AGGCCT 1 cut(s) 629
Eco24I GRGCYC 1 cut(s) 228
Eco53kI GAGCTC 1 cut(s) 226
Eco81I CCTNAGG 1 cut(s) 1040
EcoICRI GAGCTC 1 cut(s) 226
EcoT14I CCWWGG 7 cut(s) 290, 296, 312, 326, 374, 723, 960
EcoT22I ATGCAT 2 cut(s) 197, 496
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 7 cut(s) 290, 296, 312, 326, 374, 723, 960
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 4 cut(s) 45, 136, 659, 812
FauI CCCGC 2 cut(s) 254, 883
FauNDI CATATG 1 cut(s) 732
Fnu4HI GCNGC 5 cut(s) 17, 39, 102, 411, 745
FokI GGATG 4 cut(s) 265, 295, 401, 917
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 5 cut(s) 17, 39, 102, 411, 745
FspBI CTAG 3 cut(s) 87, 375, 852
GlaI GCGC 3 cut(s) 20, 560, 817
GluI GCNGC 5 cut(s) 17, 39, 102, 411, 745
HaeIII GGCC 7 cut(s) 41, 295, 325, 372, 379, 629, 1035
HapII CCGG 3 cut(s) 467, 636, 665
HgaI GACGC 3 cut(s) 56, 343, 628
HhaI GCGC 3 cut(s) 21, 561, 818
Hin1I GRCGYC 2 cut(s) 335, 639
Hin6I GCGC 3 cut(s) 19, 559, 816
HinP1I GCGC 3 cut(s) 19, 559, 816
HindIII AAGCTT 2 cut(s) 552, 1083
HinfI GANTC 2 cut(s) 470, 800
HpaII CCGG 3 cut(s) 467, 636, 665
HphI GGTGA 5 cut(s) 596, 598, 694, 710, 1004
Hpy166II GTNNAC 1 cut(s) 767
Hpy188I TCNGA 8 cut(s) 188, 254, 424, 475, 531, 717, 1006, 1027
Hpy188III TCNNGA 2 cut(s) 96, 1054
Hpy8I GTNNAC 1 cut(s) 767
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 3 cut(s) 258, 385, 740
HpyCH4IV ACGT 1 cut(s) 109
HpyCH4V TGCA 8 cut(s) 4, 137, 195, 494, 747, 767, 883, 971
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 98, 416, 1067
HpyF3I CTNAG 3 cut(s) 528, 1040, 1071
HpySE526I ACGT 1 cut(s) 109
Hsp92I GRCGYC 2 cut(s) 335, 639
HspAI GCGC 3 cut(s) 19, 559, 816
KroI GCCGGC 1 cut(s) 635
KroNI GCCGGC 1 cut(s) 637
Kzo9I GATC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 223, 813
Lsp1109I GCAGC 1 cut(s) 731
LweI GCATC 8 cut(s) 204, 287, 326, 481, 847, 870, 958, 985
MaeI CTAG 3 cut(s) 87, 375, 852
MaeII ACGT 1 cut(s) 109
MaeIII GTNAC 2 cut(s) 105, 1009
MalI GATC 1 cut(s) 166
MbiI CCGCTC 1 cut(s) 101
MboI GATC 1 cut(s) 164
MboII GAAGA 5 cut(s) 20, 228, 441, 493, 1081
MflI RGATCY 1 cut(s) 164
MhlI GDGCHC 2 cut(s) 228, 769
MlsI TGGCCA 2 cut(s) 295, 325
MluCI AATT 5 cut(s) 129, 144, 602, 652, 686
MluNI TGGCCA 2 cut(s) 295, 325
MlyI GAGTC 1 cut(s) 794
MmeI TCCRAC 1 cut(s) 447
Mox20I TGGCCA 2 cut(s) 295, 325
Mph1103I ATGCAT 2 cut(s) 197, 496
MroNI GCCGGC 1 cut(s) 635
MscI TGGCCA 2 cut(s) 295, 325
MseI TTAA 3 cut(s) 128, 579, 873
MslI CAYNNNNRTG 2 cut(s) 295, 325
Msp20I TGGCCA 2 cut(s) 295, 325
MspI CCGG 3 cut(s) 467, 636, 665
MvnI CGCG 2 cut(s) 19, 818
MwoI GCNNNNNNNGC 4 cut(s) 47, 98, 416, 1067
NaeI GCCGGC 1 cut(s) 637
NcoI CCATGG 6 cut(s) 290, 296, 312, 326, 723, 960
NdeI CATATG 1 cut(s) 732
NdeII GATC 1 cut(s) 164
NgoMIV GCCGGC 1 cut(s) 635
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 1009
NsiI ATGCAT 2 cut(s) 197, 496
PceI AGGCCT 1 cut(s) 629
PcsI WCGNNNNNNNCGW 1 cut(s) 815
PdiI GCCGGC 1 cut(s) 637
PfeI GAWTC 1 cut(s) 470
PflMI CCANNNNNTGG 1 cut(s) 119
PkrI GCNGC 5 cut(s) 18, 40, 103, 412, 746
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
PshBI ATTAAT 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 371
PstI CTGCAG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 164
RsaI GTAC 1 cut(s) 737
RsaNI GTAC 1 cut(s) 736
RseI CAYNNNNRTG 2 cut(s) 295, 325
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 3 cut(s) 128, 579, 873
SatI GCNGC 5 cut(s) 17, 39, 102, 411, 745
Sau3AI GATC 1 cut(s) 164
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 1 cut(s) 794
SduI GDGCHC 2 cut(s) 228, 769
SfaNI GCATC 8 cut(s) 204, 287, 326, 481, 847, 870, 958, 985
SfcI CTRYAG 1 cut(s) 745
SmiMI CAYNNNNRTG 2 cut(s) 295, 325
SmlI CTYRAG 3 cut(s) 94, 623, 1054
SmoI CTYRAG 3 cut(s) 94, 623, 1054
Sse9I AATT 5 cut(s) 129, 144, 602, 652, 686
SseBI AGGCCT 1 cut(s) 629
SsiI CCGC 8 cut(s) 17, 38, 101, 261, 411, 417, 860, 890
SspMI CTAG 3 cut(s) 87, 375, 852
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 629
StyI CCWWGG 7 cut(s) 290, 296, 312, 326, 374, 723, 960
TaaI ACNGT 3 cut(s) 258, 385, 740
TaiI ACGT 1 cut(s) 112
TaqI TCGA 3 cut(s) 228, 246, 944
TasI AATT 5 cut(s) 129, 144, 602, 652, 686
TatI WGTACW 1 cut(s) 735
TauI GCSGC 4 cut(s) 19, 41, 104, 413
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 3 cut(s) 128, 579, 873
Tru9I TTAA 3 cut(s) 128, 579, 873
TscAI CASTG 1 cut(s) 754
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 1 cut(s) 744
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 4 cut(s) 659, 719, 798, 965
TspGWI ACGGA 1 cut(s) 917
TspRI CASTG 1 cut(s) 754
Van91I CCANNNNNTGG 1 cut(s) 119
VneI GTGCAC 1 cut(s) 765
VspI ATTAAT 1 cut(s) 128
XapI RAATTY 3 cut(s) 602, 652, 686
XmaJI CCTAGG 1 cut(s) 374
XspI CTAG 3 cut(s) 87, 375, 852
Zsp2I ATGCAT 2 cut(s) 197, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.