Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002842

Physical Location & Seq

Genomic Coordinates Reverse (-)

24601696 .. 24610094

Gene Structure 8399 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1164700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2544 bp

ATGAGCCGTTTAGGGTTTAAGTCCGTGGTCTACCATGGCGAGCTGTGTTTGGGGGAGCTGGACGCTATTCCGGTGACGGAGGGGAGTTTCCAGTTCCCAAACAATGAGATTCGAATCCACCGGATTTCGCAGCAAAGCGAGAGGTGTCCTCCCCTCTCGATTCTCCAGACGATTTCTTCGTTTTCGGTTCGATGCAAACTCGAATCGTCCTCCCCTGTCGAACAGCCGCATTTGATCAATCTCCACGCCTCCTGTTTCTATGAGTTCAAGACGGCGACTGTGTTGGTTGGGGATGAAGAGATTCACTTGGTAGCCATGCCGAGTAAGCAGAAGAAATTTCCGTGCTTTTGGTGCTATGCGGTTCCTCTAGGTCTGTACAGTGCTTCTCTAGGAATGCTGAATCTGAGGGTTCTTTCAATTGTGTTTGATCTCGATGAGACGTTGATTGTTGCCAACACCATGAAGTCTTTTGAGGATAGAGTTGAGACTTTACGAAGCTGGGTTGCACGCGAGAGTGATGGGGTTCGAATGGCCGGAATGTCTGCTGAAATGAAGCGGTACATGGACGATAGGTGGCTGTTGAAGCAGTACATAGATAATGATTGTGTGACGGATAATGGAAAGTTGTATAAGGTACAACTAGAGGAGGTTCCTCCACTGTCTGATAACCACGAGAAAATTGTTCGGCCTATCATTAGATTGCCAGAGAAGAACATTGTGCTCACTCGCATCAATCCTGAGAATCGCGATACCAGTGTGCTTGTGCGGTTACGACCTGCATGGGAAGATTTGAGAAGTTATTTAACTGCAAAAGGACGCAAGAGGTTTGAAGTTTACGTCTGTACTATGGCTGAAAGAGATTATGCCTTAGAAATGTGGAGGCTTCTTGACCCAGGGTCACACTTAATAGACTCAAAGCAGCTTCTGGACCGTGTTGTTTGTGTTAAATCAGGCTCCAGGAAATCTTTACTCAATGTCTTCCAACAAGGAGTGTGCCATCCAAAGATGGCAATGGTTATTGATGACCGTTCCAAGGTCTGGGAAGATAAGGATCAACCTCGAGTCCATGTTGTTCCAGCATTCACCCCTTATTATGCTCCTCAAGCAGAGACAGCTAGTGCAGTTCCAGTCCTCTGTGTAGCAAGAAATGTTGCATGCAATGTCAGAGGTTGTTTTTTCAAAGAGTTTGATGAAATTTTGTTGCGAAGAATTTCTGGAGTTTTTTATGAAGATGAGGTGGTAAATTTGCCTCCTGCTCCTGATGTGAGCAATTACTTGATGTTCGAGGATACCGGTTTTGCACCAAATGGAAATGCTAATGCTCCCATTAGTGAAGGAATGAATGGTAGCGAAGTGGAACGAAGATTGAACCCATCTGATGATAAGGGTAGCGTGGAGTCAGTGGTTCAATCTGTGAAAAATGCCGAGTCAAGGTGTGACAACTCTCAAGCACCGACTGCAATTCTTCCCAATGCTGCCGGTACATCTTTGAGGCCGGTCATGCCTTCTCAGAAACCTGGTCTGCTTGGACTTCCTGTTAGACGAGATAGCTTTTCTGATCGTGACCATGAAATGAGGAGAGGACTCCTTGGTACAACTCCTAATCTAGATATAAGGAGTCAAAGCTCTGCTGAGCCCCCTCTTCTATCTAGAGTGCCTGTACAACTGCCAGCATCCTCAATAAATGCACAAGGAGGATGGTTGGTTGATGATGACGACAACAATAGAGGACATCCGAGTAATCGCCCTTCTGGGCTTGTTCAGCAGCCTGATGTTTCAAAACCTGAAAAGTCAGTTTACCAGAATCCTTTCAGTCCTGCTAAACCAGGTTCGACACCCACTGGTTTGCTCTCACACAAATCAGATTTAAAGAGGGAAGAGATATCATCTGGGTGTGATTTGCAAAATCTTCCTGTGCCAAGTCAGCCATCAGAGGCTGGTGTATCCCAGATTCAGGCATCTTCTTTGAATATAGAATCTCAAACAGAATCTGGTAAAGTAAACCTGGTACCATCCCCTTTATCTATTGGAGTGCTGCAGGAAATTGGACGGAGGTGCAGTTCAAAGATTGAGTTCAGGTCTGTTTTAAGCACCAGCAATGACTTGCAGTTTTCTGTTGAGGTTCTTTTCACTGGAGAGAAGATAGGTTTTGGAATGGGTAGGACGAGGAAGGATGCTCAACAACAGGCTGCTGAGAATGCCCTTCACAGCTTGGCTGATAAATATGTAGCATATCTTGCACCTCGTTCTGGTGCCGTGGACAGAGATTTAAATAAAGGAAATGAAAACGGATTTCTATTGGACATGGTTGGTCCAGGATCGGCTGAGCTGCTAAGGGATGGATCGCCTAAAGAAAGAACTTCAGAGGCTGCTGAAGTTGAACCTGGAAGTACTTTTACGGTGAGTCAGCAAGTTCTAAAGCGTGCCAGTTCCCCAAGATTGATAGAGTCGGTTGCAAAACGACCCAAGGAAGACATATTGCATGGTTCACGAAGTTTATCATTTTCACGGCAGCAGAAGAATGGGGTTGCAGACAAACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF dsrm

Protein Analysis

848

Amino Acids

93.87

Weight (kDa)

6.37

Isoelectric Point (pI)

59.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	241 - 356	1.2e-07	NLI interacting factor-like phosphatase
dsrm	PF00035	676 - 738	2.1e-07	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002842)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01270 AT5G01270
fragaria_vesca	FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640
malus_domestica	MD04G1150600.v1.1 MD04G1150700.v1.1 MD12G1164700.v1.1
prunus_persica	Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1
pyrus_communis	pycom04g13630 pycom12g15760
rosa_chinensis	RchiOBHm_Chr3g0463301
rosa_laevigata	RLG00000024792
rosa_multiflora	Rmu_co8080032.1_g000001 Rmu_sc0000923.1_g000001 Rmu_sc0002976.1_g000008 Rmu_sc0033161.1_g000001
rosa_roxburghii	Rroxscaffold_6G00416690
rosa_rugosa	Rorug03G0063700 Rorug03G0063700
rosa_samantha	Rh3AG121600 Rh3BG126100 Rh3CG128000 Rh3DG127200
rosa_wichuraiana	Rw3G010330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	784
Acc65I	GGTACC	1 cut(s)	2008
AccB1I	GGYRCC	2 cut(s)	2008, 2252
AccB7I	CCANNNNNTGG	1 cut(s)	1038
AccI	GTMKAC	1 cut(s)	30
AccII	CGCG	2 cut(s)	510, 747
AciI	CCGC	4 cut(s)	227, 359, 556, 766
AclWI	GGATC	3 cut(s)	1059, 2326, 2350
AcoI	YGGCCR	1 cut(s)	531
AcsI	RAATTY	4 cut(s)	335, 1194, 1209, 1243
AcuI	CTGAAG	2 cut(s)	2346, 2394
AfiI	CCNNNNNNNGG	5 cut(s)	1033, 1038, 1431, 1954, 2249
AgeI	ACCGGT	1 cut(s)	1292
AhdI	GACNNNNNGTC	1 cut(s)	895
AjnI	CCWGG	7 cut(s)	892, 956, 1516, 1825, 2004, 2314, 2383
AloI	GAACNNNNNNTCC	2 cut(s)	2044, 2076
AluBI	AGCT	9 cut(s)	43, 58, 498, 922, 1115, 1551, 1626, 2211, 2329
AluI	AGCT	9 cut(s)	43, 58, 498, 922, 1115, 1551, 1626, 2211, 2329
Alw21I	GWGCWC	1 cut(s)	723
Alw26I	GTCTC	3 cut(s)	431, 479, 1103
AlwI	GGATC	3 cut(s)	1059, 2326, 2350
AlwNI	CAGNNNCTG	4 cut(s)	925, 1937, 1991, 2369
Ama87I	CYCGRG	1 cut(s)	1059
AoxI	GGCC	3 cut(s)	531, 686, 1493
ApeKI	GCWGC	9 cut(s)	130, 919, 1475, 1765, 2035, 2189, 2329, 2369, 2512
ApoI	RAATTY	4 cut(s)	335, 1194, 1209, 1243
ArsI	GACNNNNNNTTYG	2 cut(s)	160, 192
AsiGI	ACCGGT	1 cut(s)	1292
Asp700I	GAANNNNTTC	3 cut(s)	300, 1210, 1808
Asp718I	GGTACC	1 cut(s)	2008
AspS9I	GGNCC	2 cut(s)	928, 2312
AsuHPI	GGTGA	3 cut(s)	85, 1075, 2413
AsuII	TTCGAA	2 cut(s)	112, 526
AvaI	CYCGRG	1 cut(s)	1059
AvaII	GGWCC	2 cut(s)	928, 2312
BaeI	ACNNNNGTAYC	2 cut(s)	741, 774
BanI	GGYRCC	2 cut(s)	2008, 2252
BanII	GRGCYC	1 cut(s)	1638
BarI	GAAGNNNNNNTAC	4 cut(s)	1732, 1764, 2380, 2412
BauI	CACGAG	1 cut(s)	671
BbsI	GAAGAC	2 cut(s)	970, 2478
Bbv12I	GWGCWC	1 cut(s)	723
BbvI	GCAGC	9 cut(s)	142, 931, 1462, 1777, 2022, 2176, 2316, 2356, 2524
BccI	CCATC	8 cut(s)	512, 1000, 1005, 1381, 1692, 1936, 2020, 2333
BceAI	ACGGC	3 cut(s)	288, 2240, 2525
BciT130I	CCWGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
BciVI	GTATCC	2 cut(s)	1282, 1954
BclI	TGATCA	1 cut(s)	234
BcoDI	GTCTC	3 cut(s)	431, 479, 1103
BfaI	CTAG	6 cut(s)	368, 389, 641, 1116, 1607, 1650
BfmI	CTRYAG	1 cut(s)	2036
BfuAI	ACCTGC	1 cut(s)	784
BfuI	GTATCC	2 cut(s)	1282, 1954
BlpI	GCTNAGC	2 cut(s)	1632, 2325
BmcAI	AGTACT	1 cut(s)	2392
Bme1390I	CCNGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
Bme18I	GGWCC	2 cut(s)	928, 2312
BmeRI	GACNNNNNGTC	1 cut(s)	895
BmeT110I	CYCGRG	1 cut(s)	1059
BmgT120I	GGNCC	2 cut(s)	928, 2312
BmiI	GGNNCC	5 cut(s)	363, 651, 955, 2010, 2254
BmrFI	CCNGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
BmsI	GCATC	5 cut(s)	182, 738, 1682, 1967, 2164
BpiI	GAAGAC	2 cut(s)	970, 2478
BplI	GAGNNNNNCTC	2 cut(s)	133, 165
BpmI	CTGGAG	4 cut(s)	149, 940, 1236, 2154
Bpu10I	CCTNAGC	1 cut(s)	2333
Bpu1102I	GCTNAGC	2 cut(s)	1632, 2325
Bpu14I	TTCGAA	2 cut(s)	112, 526
BpuEI	CTTGAG	2 cut(s)	1086, 1431
BsaBI	GATNNNNATC	2 cut(s)	113, 1050
BsaJI	CCNNGG	8 cut(s)	24, 34, 892, 893, 1032, 1588, 2256, 2466
BsaWI	WCCGGW	3 cut(s)	70, 120, 1292
BsaXI	ACNNNNNCTCC	2 cut(s)	2044, 2074
Bsc4I	CCNNNNNNNGG	5 cut(s)	1033, 1038, 1431, 1954, 2249
Bse118I	RCCGGY	3 cut(s)	1292, 1478, 1495
Bse1I	ACTGG	6 cut(s)	91, 753, 1127, 1846, 2137, 2427
Bse3DI	GCAATG	3 cut(s)	1017, 1165, 2104
Bse8I	GATNNNNATC	2 cut(s)	113, 1050
BseBI	CCWGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
BseDI	CCNNGG	8 cut(s)	24, 34, 892, 893, 1032, 1588, 2256, 2466
BseGI	GGATG	8 cut(s)	298, 997, 1673, 1703, 1732, 2012, 2179, 2344
BseJI	GATNNNNATC	2 cut(s)	113, 1050
BseLI	CCNNNNNNNGG	5 cut(s)	1033, 1038, 1431, 1954, 2249
BseMI	GCAATG	3 cut(s)	1017, 1165, 2104
BseMII	CTCAG	6 cut(s)	395, 729, 1523, 1623, 2184, 2316
BseNI	ACTGG	6 cut(s)	91, 753, 1127, 1846, 2137, 2427
BseRI	GAGGAG	3 cut(s)	659, 1089, 1591
BseXI	GCAGC	9 cut(s)	142, 931, 1462, 1777, 2022, 2176, 2316, 2356, 2524
BseYI	CCCAGC	1 cut(s)	498
BsgI	GTGCAG	2 cut(s)	1140, 2077
Bsh1236I	CGCG	2 cut(s)	510, 747
BshFI	GGCC	3 cut(s)	533, 688, 1495
BshNI	GGYRCC	2 cut(s)	2008, 2252
BshTI	ACCGGT	1 cut(s)	1292
BsiHKAI	GWGCWC	1 cut(s)	723
BsiHKCI	CYCGRG	1 cut(s)	1059
BsiSI	CCGG	6 cut(s)	71, 121, 534, 1293, 1479, 1496
BslI	CCNNNNNNNGG	5 cut(s)	1033, 1038, 1431, 1954, 2249
BsmAI	GTCTC	3 cut(s)	431, 479, 1103
BsmBI	CGTCTC	1 cut(s)	431
BsmI	GAATGC	3 cut(s)	399, 1079, 2203
BsnI	GGCC	3 cut(s)	533, 688, 1495
BsoBI	CYCGRG	1 cut(s)	1059
Bsp119I	TTCGAA	2 cut(s)	112, 526
Bsp1286I	GDGCHC	2 cut(s)	723, 1638
Bsp1407I	TGTACA	2 cut(s)	375, 1660
Bsp143I	GATC	6 cut(s)	234, 427, 1051, 1558, 2318, 2342
Bsp1720I	GCTNAGC	2 cut(s)	1632, 2325
Bsp19I	CCATGG	1 cut(s)	34
Bsp68I	TCGCGA	1 cut(s)	747
BspACI	CCGC	4 cut(s)	227, 359, 556, 766
BspANI	GGCC	3 cut(s)	533, 688, 1495
BspCNI	CTCAG	6 cut(s)	396, 730, 1522, 1624, 2185, 2317
BspFNI	CGCG	2 cut(s)	510, 747
BspLI	GGNNCC	5 cut(s)	363, 651, 955, 2010, 2254
BspMAI	CTGCAG	1 cut(s)	2040
BspMI	ACCTGC	1 cut(s)	784
BspPI	GGATC	3 cut(s)	1059, 2326, 2350
BspT104I	TTCGAA	2 cut(s)	112, 526
BspT107I	GGYRCC	2 cut(s)	2008, 2252
BsrDI	GCAATG	3 cut(s)	1017, 1165, 2104
BsrFI	RCCGGY	3 cut(s)	1292, 1478, 1495
BsrGI	TGTACA	2 cut(s)	375, 1660
BsrI	ACTGG	6 cut(s)	91, 753, 1127, 1846, 2137, 2427
BssAI	RCCGGY	3 cut(s)	1292, 1478, 1495
BssECI	CCNNGG	8 cut(s)	24, 34, 892, 893, 1032, 1588, 2256, 2466
BssMI	GATC	6 cut(s)	234, 427, 1051, 1558, 2318, 2342
BssSI	CACGAG	1 cut(s)	671
BssT1I	CCWWGG	4 cut(s)	34, 1032, 1588, 2466
Bst2BI	CACGAG	1 cut(s)	671
Bst2UI	CCWGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
Bst4CI	ACNGT	6 cut(s)	280, 380, 660, 932, 1028, 2401
Bst6I	CTCTTC	3 cut(s)	291, 1647, 1872
BstAPI	GCANNNNNTGC	2 cut(s)	1457, 2237
BstAUI	TGTACA	2 cut(s)	375, 1660
BstBI	TTCGAA	2 cut(s)	112, 526
BstC8I	GCNNGC	5 cut(s)	41, 508, 1156, 1671, 2424
BstDEI	CTNAG	8 cut(s)	404, 738, 868, 1509, 1632, 2193, 2325, 2333
BstDSI	CCRYGG	3 cut(s)	24, 34, 2256
BstF5I	GGATG	8 cut(s)	298, 997, 1673, 1703, 1732, 2012, 2179, 2344
BstFNI	CGCG	2 cut(s)	510, 747
BstKTI	GATC	6 cut(s)	237, 430, 1054, 1561, 2321, 2345
BstMAI	GTCTC	3 cut(s)	431, 479, 1103
BstMBI	GATC	6 cut(s)	234, 427, 1051, 1558, 2318, 2342
BstNI	CCWGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
BstNSI	RCATGY	1 cut(s)	1158
BstSCI	CCNGG	7 cut(s)	892, 956, 1516, 1825, 2004, 2314, 2383
BstSFI	CTRYAG	1 cut(s)	2036
BstUI	CGCG	2 cut(s)	510, 747
BstV1I	GCAGC	9 cut(s)	142, 931, 1462, 1777, 2022, 2176, 2316, 2356, 2524
BstV2I	GAAGAC	2 cut(s)	970, 2478
BsuI	GTATCC	2 cut(s)	1282, 1954
BsuRI	GGCC	3 cut(s)	533, 688, 1495
BtgI	CCRYGG	3 cut(s)	24, 34, 2256
BtsCI	GGATG	8 cut(s)	298, 997, 1673, 1703, 1732, 2012, 2179, 2344
BtsIMutI	CAGTG	6 cut(s)	385, 656, 760, 1407, 1839, 2130
BtuMI	TCGCGA	1 cut(s)	747
BveI	ACCTGC	1 cut(s)	784
Cac8I	GCNNGC	5 cut(s)	41, 508, 1156, 1671, 2424
CaiI	CAGNNNCTG	4 cut(s)	925, 1937, 1991, 2369
Cfr10I	RCCGGY	3 cut(s)	1292, 1478, 1495
Cfr13I	GGNCC	2 cut(s)	928, 2312
CseI	GACGC	2 cut(s)	71, 825
CsiI	ACCWGGT	3 cut(s)	1516, 1825, 2004
CspAI	ACCGGT	1 cut(s)	1292
DdeI	CTNAG	8 cut(s)	404, 738, 868, 1509, 1632, 2193, 2325, 2333
DpnI	GATC	6 cut(s)	236, 429, 1053, 1560, 2320, 2344
DpnII	GATC	6 cut(s)	234, 427, 1051, 1558, 2318, 2342
DraI	TTTAAA	2 cut(s)	1869, 2271
DriI	GACNNNNNGTC	1 cut(s)	895
EaeI	YGGCCR	1 cut(s)	531
Eam1104I	CTCTTC	3 cut(s)	291, 1647, 1872
Eam1105I	GACNNNNNGTC	1 cut(s)	895
EarI	CTCTTC	3 cut(s)	291, 1647, 1872
Eco130I	CCWWGG	4 cut(s)	34, 1032, 1588, 2466
Eco24I	GRGCYC	1 cut(s)	1638
Eco32I	GATATC	1 cut(s)	1884
Eco47I	GGWCC	2 cut(s)	928, 2312
Eco57I	CTGAAG	2 cut(s)	2346, 2394
Eco88I	CYCGRG	1 cut(s)	1059
EcoRII	CCWGG	7 cut(s)	892, 956, 1516, 1825, 2004, 2314, 2383
EcoRV	GATATC	1 cut(s)	1884
EcoT14I	CCWWGG	4 cut(s)	34, 1032, 1588, 2466
EcoT38I	GRGCYC	1 cut(s)	1638
ErhI	CCWWGG	4 cut(s)	34, 1032, 1588, 2466
Esp3I	CGTCTC	1 cut(s)	431
FbaI	TGATCA	1 cut(s)	234
FblI	GTMKAC	1 cut(s)	30
FokI	GGATG	8 cut(s)	305, 984, 1660, 1710, 1719, 1999, 2186, 2351
FriOI	GRGCYC	1 cut(s)	1638
FspBI	CTAG	6 cut(s)	368, 389, 641, 1116, 1607, 1650
GsaI	CCCAGC	1 cut(s)	502
GsuI	CTGGAG	4 cut(s)	149, 940, 1236, 2154
HaeIII	GGCC	3 cut(s)	533, 688, 1495
HapII	CCGG	6 cut(s)	71, 121, 534, 1293, 1479, 1496
HgaI	GACGC	2 cut(s)	71, 825
HpaII	CCGG	6 cut(s)	71, 121, 534, 1293, 1479, 1496
HphI	GGTGA	3 cut(s)	85, 1075, 2413
Hpy166II	GTNNAC	6 cut(s)	31, 835, 1798, 2002, 2260, 2489
Hpy8I	GTNNAC	6 cut(s)	31, 835, 1798, 2002, 2260, 2489
HpyAV	CCTTC	5 cut(s)	1328, 1515, 1758, 2164, 2213
HpyCH4III	ACNGT	6 cut(s)	280, 380, 660, 932, 1028, 2401
HpyCH4IV	ACGT	2 cut(s)	440, 837
HpyF3I	CTNAG	8 cut(s)	404, 738, 868, 1509, 1632, 2193, 2325, 2333
HpySE526I	ACGT	2 cut(s)	440, 837
KpnI	GGTACC	1 cut(s)	2012
Ksp22I	TGATCA	1 cut(s)	234
Kzo9I	GATC	6 cut(s)	234, 427, 1051, 1558, 2318, 2342
LmnI	GCTCC	5 cut(s)	55, 959, 1102, 1261, 1327
Lsp1109I	GCAGC	9 cut(s)	142, 931, 1462, 1777, 2022, 2176, 2316, 2356, 2524
LweI	GCATC	5 cut(s)	182, 738, 1682, 1967, 2164
MabI	ACCWGGT	3 cut(s)	1516, 1825, 2004
MaeI	CTAG	6 cut(s)	368, 389, 641, 1116, 1607, 1650
MaeII	ACGT	2 cut(s)	440, 837
MaeIII	GTNAC	6 cut(s)	73, 607, 768, 897, 1436, 1562
MalI	GATC	6 cut(s)	236, 429, 1053, 1560, 2320, 2344
MboI	GATC	6 cut(s)	234, 427, 1051, 1558, 2318, 2342
MfeI	CAATTG	1 cut(s)	417
MhlI	GDGCHC	2 cut(s)	723, 1638
MluCI	AATT	9 cut(s)	335, 417, 678, 1194, 1209, 1243, 1270, 1461, 2043
MlyI	GAGTC	8 cut(s)	905, 1071, 1406, 1436, 1578, 1627, 2413, 2456
MmeI	TCCRAC	1 cut(s)	1006
MroXI	GAANNNNTTC	3 cut(s)	300, 1210, 1808
MseI	TTAA	7 cut(s)	18, 803, 905, 945, 1868, 2087, 2270
MslI	CAYNNNNRTG	1 cut(s)	1891
MspI	CCGG	6 cut(s)	71, 121, 534, 1293, 1479, 1496
MspR9I	CCNGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
MunI	CAATTG	1 cut(s)	417
Mva1269I	GAATGC	3 cut(s)	399, 1079, 2203
MvaI	CCWGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
MvnI	CGCG	2 cut(s)	510, 747
NcoI	CCATGG	1 cut(s)	34
NdeII	GATC	6 cut(s)	234, 427, 1051, 1558, 2318, 2342
NlaIV	GGNNCC	5 cut(s)	363, 651, 955, 2010, 2254
NmeAIII	GCCGAG	2 cut(s)	345, 1450
NmuCI	GTSAC	5 cut(s)	73, 607, 897, 1436, 1562
NruI	TCGCGA	1 cut(s)	747
NspI	RCATGY	1 cut(s)	1158
NspV	TTCGAA	2 cut(s)	112, 526
PaeI	GCATGC	1 cut(s)	1158
PaeR7I	CTCGAG	1 cut(s)	1059
PasI	CCCWGGG	1 cut(s)	893
PcsI	WCGNNNNNNNCGW	1 cut(s)	176
PctI	GAATGC	3 cut(s)	399, 1079, 2203
PdmI	GAANNNNTTC	3 cut(s)	300, 1210, 1808
PflMI	CCANNNNNTGG	1 cut(s)	1038
PfoI	TCCNGGA	2 cut(s)	956, 2314
PinAI	ACCGGT	1 cut(s)	1292
PleI	GAGTC	8 cut(s)	905, 1070, 1405, 1435, 1578, 1626, 2412, 2455
PpsI	GAGTC	8 cut(s)	905, 1070, 1405, 1435, 1578, 1626, 2412, 2455
Psp6I	CCWGG	7 cut(s)	892, 956, 1516, 1825, 2004, 2314, 2383
PspFI	CCCAGC	1 cut(s)	498
PspGI	CCWGG	7 cut(s)	892, 956, 1516, 1825, 2004, 2314, 2383
PspN4I	GGNNCC	5 cut(s)	363, 651, 955, 2010, 2254
PspPI	GGNCC	2 cut(s)	928, 2312
PspXI	VCTCGAGB	1 cut(s)	1059
PstI	CTGCAG	1 cut(s)	2040
PstNI	CAGNNNCTG	4 cut(s)	925, 1937, 1991, 2369
RruI	TCGCGA	1 cut(s)	747
RseI	CAYNNNNRTG	1 cut(s)	1891
SaqAI	TTAA	7 cut(s)	18, 803, 905, 945, 1868, 2087, 2270
Sau3AI	GATC	6 cut(s)	234, 427, 1051, 1558, 2318, 2342
Sau96I	GGNCC	2 cut(s)	928, 2312
ScaI	AGTACT	1 cut(s)	2392
SchI	GAGTC	8 cut(s)	905, 1071, 1406, 1436, 1578, 1627, 2413, 2456
ScrFI	CCNGG	7 cut(s)	894, 958, 1518, 1827, 2006, 2316, 2385
SduI	GDGCHC	2 cut(s)	723, 1638
SexAI	ACCWGGT	3 cut(s)	1516, 1825, 2004
SfaNI	GCATC	5 cut(s)	182, 738, 1682, 1967, 2164
SfcI	CTRYAG	1 cut(s)	2036
Sfr274I	CTCGAG	1 cut(s)	1059
SfuI	TTCGAA	2 cut(s)	112, 526
SinI	GGWCC	2 cut(s)	928, 2312
SlaI	CTCGAG	1 cut(s)	1059
SmiI	ATTTAAAT	1 cut(s)	2271
SmiMI	CAYNNNNRTG	1 cut(s)	1891
SmlI	CTYRAG	3 cut(s)	1059, 1101, 1446
SmoI	CTYRAG	3 cut(s)	1059, 1101, 1446
SphI	GCATGC	1 cut(s)	1158
Sse9I	AATT	9 cut(s)	335, 417, 678, 1194, 1209, 1243, 1270, 1461, 2043
SsiI	CCGC	4 cut(s)	227, 359, 556, 766
SspMI	CTAG	6 cut(s)	368, 389, 641, 1116, 1607, 1650
StyD4I	CCNGG	7 cut(s)	892, 956, 1516, 1825, 2004, 2314, 2383
StyI	CCWWGG	4 cut(s)	34, 1032, 1588, 2466
SwaI	ATTTAAAT	1 cut(s)	2271
TaaI	ACNGT	6 cut(s)	280, 380, 660, 932, 1028, 2401
TaiI	ACGT	2 cut(s)	443, 840
TasI	AATT	9 cut(s)	335, 417, 678, 1194, 1209, 1243, 1270, 1461, 2043
TatI	WGTACW	5 cut(s)	375, 588, 842, 1660, 2390
TauI	GCSGC	1 cut(s)	229
Tru1I	TTAA	7 cut(s)	18, 803, 905, 945, 1868, 2087, 2270
Tru9I	TTAA	7 cut(s)	18, 803, 905, 945, 1868, 2087, 2270
TscAI	CASTG	6 cut(s)	385, 663, 760, 1407, 1846, 2137
TseFI	GTSAC	5 cut(s)	73, 607, 897, 1436, 1562
TseI	GCWGC	9 cut(s)	130, 919, 1475, 1765, 2035, 2189, 2329, 2369, 2512
Tsp45I	GTSAC	5 cut(s)	73, 607, 897, 1436, 1562
TspDTI	ATGAA	8 cut(s)	309, 476, 566, 1206, 1242, 1355, 1584, 2298
TspGWI	ACGGA	6 cut(s)	13, 92, 330, 626, 2065, 2304
TspRI	CASTG	6 cut(s)	385, 663, 760, 1407, 1846, 2137
Van91I	CCANNNNNTGG	1 cut(s)	1038
VpaK11BI	GGWCC	2 cut(s)	928, 2312
XapI	RAATTY	4 cut(s)	335, 1194, 1209, 1243
XbaI	TCTAGA	2 cut(s)	1606, 1649
XceI	RCATGY	1 cut(s)	1158
XhoI	CTCGAG	1 cut(s)	1059
XmiI	GTMKAC	1 cut(s)	30
XmnI	GAANNNNTTC	3 cut(s)	300, 1210, 1808
XspI	CTAG	6 cut(s)	368, 389, 641, 1116, 1607, 1650
ZrmI	AGTACT	1 cut(s)	2392

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.