Rroxscaffold_6G00416690

RNA polymerase II C-terminal domain phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002842
Physical Location & Seq
Reverse (-)
38618549 .. 38625377
6829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00416690.1

Sequence Viewer

Length: 2451 bp
ATGAGCAGTTTAGGGTTTAGGTCCGTGGTCTATCACGGCGAGCTGTGCTTGGGTGAGCTCGACGCTATTCCGGTGACGGAGGGGAAATTTCAGTTCCCGAACAACGAGATTCGAATCCACCACATATCGCAGCAAAGTGAACGCTGTCCACCCCTCTCAGTTCTCCAAACGATTTCCTCATTTTCGGTTAGATGCAAGCTCGAATCGGCCACCCCTGTTGAACAGCCTCATTTGATCAACCTCCACGCCTCGTGTTTCTACGATCTCAAGACGGCGGTAGTGTTGATTGGGAATGAAGAGATTCACTTGGTGGCAATGCCAAGTAAGCAGAAAAAGTTTCCGTGCTTTTGGTGCTATTCGGTTCCTTTAGGTCTGTATAGTGCTTCTCTTGGAATGCTGAATCCAAGGTGTCTCTCAATTGTATTTGATCTCGACGAGACTCTAATTGTTGCCAACACAATGAAGTCGTTTGAGGATAGAATTGAGGCTTTACGAAGCTGGATTGCACGAGAGAGTGAAACGTCGCGAATAGCCGGAATGTCTAATGAAATGAAACGTTACATTGACGATAGGTGGTTGTTGAGGCAGTATATAGATAATGATTGTGTGGTGGATAACGGACAGGTATATAAGATTCAAATGGAGGAGGTTCCTCCACTGTCAGGTAACCATGAGAGAATCCGTCGGCCGATCGTTAGATTGCCAGATAGGAACATCGTGCTCACTCGCATCAATCCCGAGGTCTGGAAGACTCCTATGCTTGTTCGTGATACAAGTGTGCTGGTCAGGTTACGGCCTGCATGGGAAGATCTGAGAAGTTATTTAACTGCAAAAGGACGCAAGCGGTTTGAAGTTCATGTCTGTACGATGGCTGAAAGGGACTATGCATTAGAAATGTGGAGGCTTCTAGACCCAGAAGGACAACTAATAGGTTCAAAGCAACTTCTTGACCGTGTTGTTTGTGTCAAATCAGGTTCGCGGAAATCTTTACTCAATGTCTTCCAACTTGGAACGTGCCATCCAAAGATGGCCATGGTTATTGATGATCGTTCAAAGGTCTGGGAAGATAAGGATCAACCTCGAGTTCATGTAGTTCCAGCTTTTACTCCTTACTATGCTCCTCAAGCAGAGACAGCAAATGCCGTTCCTGTCCTCTGTGTAGCAAGAAATGTTGCATGCAATGTCAGGGGCTGCTTTTTTAAAGAGTTCGATGAGATTTTCTTGCGAAGAATTTCTGAAGTTTTTTATGAAGATGAGGTGGTAAATTTACCTCCTTCTCCTGATGTGAGCAATTACTTGATATCAGAGGATGCTGGTTTTGTACCAAATGGAATTGCTCCCTTTAGTGAAGGAATGCATGGTGGTGAAGTTGAACGGAGATTGAACCAACCTGATGATAGGGGTGTTATGGACTCAATGGCTTATTCTAATCATGCTGAGTTAAGACCTGACAACTCCCAAAAACCTGTAGCAACTGTCCTCAATGTCCCTGGTGCAACATCTTTGAGACCGGTCATGCCTACTCAGAAACCTAGTTTGCTTGGACCTCTTGTTAGACGAGACAGCTTTTTTGATCGTGATAATAACATGAAGAGAGGACTTCTTGGTACAAATCCTTGCCTAGATATAAGGAGTCAAAGCTCCGGTGAACCCCTAAATTCATCTAGAGTACCTGCACAAACTTCAGCATCCACAGTACATGCACAAGGAGGATGGTTGGTGGATGATGACAAGAAAAGAGGACATTTAAGTTTTCGACCTCCTGGTTCTGGTCAAGAGTCTGATGTCTTAAAACCTGAGAAGCTGATCCACCAGAATCCGTTTAGTCTTAATAAACAGTCTGCTCCGACTGGTTTACTCTCACAGACATCTGAGGCAAGGAGGGGAAGAGAGGCTGGTGTGTCTCAGAATCAGGCATCTTCTTTTAAAAAAGAATCTGGAAAAGTGAACGCGCTGCCGTCCCCTTTATCTATTGGTGTGCTGCAGGAAATCGGACGGAGATGCGGCTCAAAGGTGCTCTTCACTGGGGAGAAGATAGGTTTTGGAACAGGAAGGACAAGAAAGGATGCTCAACAACAGGCTGCTGAGAATGCACTTCACAGCTTGGCTGAAAAATATGTCTCATATATTGCACCGCAATCTGGAGCAGTGGACAAAGATTTTGATAAACTCTCTCTTGGAAATGAAAACGGATTTGTTTTGGACATAGTTGGTCCAGGATCAGCTGAGGGGCTAACGGAAGATGGATTGCCAAAAGAAAGTACTTCTGAGGCGTCTGAAGGTGAGCCTGGGAGCTCTTCTACTGTGGCGAGTCAGCAAGTTCAAAAGCGTGCCAATTCCCCAAGGTTGCTACAATCAGTTTCGAATAAACGATCCAAGGAAGAACTAGCGGGCGGTCCACGAAACCTGTCATATACTCGGCTGAGGAAGGGCCCTAAAGACATACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF dsrm

Protein Analysis

816

Amino Acids

90.71

Weight (kDa)

8.21

Isoelectric Point (pI)

55.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 255 - 363 1.5e-07 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1681
AccII CGCG 3 cut(s) 526, 979, 1952
AciI CCGC 7 cut(s) 275, 844, 979, 2004, 2135, 2390, 2394
AclI AACGTT 1 cut(s) 556
AclWI GGATC 4 cut(s) 1080, 1801, 2227, 2367
AcoI YGGCCR 3 cut(s) 207, 686, 1029
AcsI RAATTY 4 cut(s) 86, 1230, 1264, 1657
AcuI CTGAAG 3 cut(s) 1257, 1668, 2298
AcyI GRCGYC 1 cut(s) 2273
AdeI CACNNNGTG 1 cut(s) 310
AfaI GTAC 6 cut(s) 865, 1323, 1609, 1671, 1698, 2263
AfiI CCNNNNNNNGG 4 cut(s) 662, 744, 1769, 2141
AgeI ACCGGT 1 cut(s) 1510
AgsI TTSAA 8 cut(s) 221, 638, 851, 936, 1053, 1373, 1384, 2324
AjnI CCWGG 4 cut(s) 1489, 1762, 2215, 2287
AloI GAACNNNNNNTCC 2 cut(s) 1003, 1035
Alw21I GWGCWC 4 cut(s) 60, 723, 2019, 2297
Alw26I GTCTC 7 cut(s) 416, 431, 1124, 1501, 1554, 1908, 2125
AlwI GGATC 4 cut(s) 1080, 1801, 2227, 2367
AlwNI CAGNNNCTG 1 cut(s) 1191
Ama87I CYCGRG 2 cut(s) 737, 1080
AoxI GGCC 5 cut(s) 207, 686, 794, 1029, 2431
ApaI GGGCCC 1 cut(s) 2435
ApeKI GCWGC 5 cut(s) 130, 1191, 1954, 1981, 2081
ApoI RAATTY 4 cut(s) 86, 1230, 1264, 1657
ArsI GACNNNNNNTTYG 4 cut(s) 1553, 1585, 2144, 2176
AsiGI ACCGGT 1 cut(s) 1510
Asp700I GAANNNNTTC 2 cut(s) 300, 1231
AspLEI GCGC 1 cut(s) 1954
AspS9I GGNCC 6 cut(s) 21, 1544, 2213, 2396, 2431, 2432
AsuHPI GGTGA 5 cut(s) 65, 85, 1376, 1658, 2294
AsuII TTCGAA 2 cut(s) 112, 2363
AvaI CYCGRG 2 cut(s) 737, 1080
AvaII GGWCC 4 cut(s) 21, 1544, 2213, 2396
BaeGI GKGCMC 1 cut(s) 2435
BaeI ACNNNNGTAYC 2 cut(s) 762, 795
BalI TGGCCA 1 cut(s) 1031
BanII GRGCYC 3 cut(s) 60, 2297, 2435
BauI CACGAG 2 cut(s) 250, 507
BbsI GAAGAC 2 cut(s) 755, 991
Bbv12I GWGCWC 4 cut(s) 60, 723, 2019, 2297
BbvCI CCTCAGC 2 cut(s) 2226, 2423
BbvI GCAGC 5 cut(s) 142, 1178, 1941, 1968, 2068
BccI CCATC 5 cut(s) 862, 1021, 1026, 1707, 2237
BceAI ACGGC 5 cut(s) 52, 288, 809, 1127, 1942
BciT130I CCWGG 4 cut(s) 1491, 1764, 2217, 2289
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 7 cut(s) 416, 431, 1124, 1501, 1554, 1908, 2125
BfaI CTAG 5 cut(s) 908, 1533, 1622, 1665, 2387
BfmI CTRYAG 2 cut(s) 1467, 1982
BfuAI ACCTGC 1 cut(s) 1681
BglII AGATCT 1 cut(s) 808
BisI GCNGC 6 cut(s) 131, 1192, 1955, 1982, 2005, 2082
BlsI GCNGC 6 cut(s) 132, 1193, 1956, 1983, 2006, 2083
BmcAI AGTACT 1 cut(s) 2263
Bme1390I CCNGG 4 cut(s) 1491, 1764, 2217, 2289
Bme18I GGWCC 4 cut(s) 21, 1544, 2213, 2396
BmeT110I CYCGRG 2 cut(s) 737, 1080
BmgT120I GGNCC 6 cut(s) 21, 1544, 2213, 2396, 2431, 2432
BmiI GGNNCC 3 cut(s) 363, 651, 2433
BmrFI CCNGG 4 cut(s) 1491, 1764, 2217, 2289
BmrI ACTGGG 1 cut(s) 2034
BmsI GCATC 7 cut(s) 182, 738, 1300, 1697, 1925, 1991, 2056
BmuI ACTGGG 1 cut(s) 2034
BpiI GAAGAC 2 cut(s) 755, 991
BpmI CTGGAG 1 cut(s) 2163
Bpu10I CCTNAGC 2 cut(s) 2226, 2423
Bpu14I TTCGAA 2 cut(s) 112, 2363
BpuEI CTTGAG 2 cut(s) 251, 1107
BsaBI GATNNNNATC 2 cut(s) 113, 1071
BsaHI GRCGYC 1 cut(s) 2273
BsaI GGTCTC 1 cut(s) 1501
BsaJI CCNNGG 8 cut(s) 24, 404, 738, 1032, 1489, 2288, 2342, 2376
BsaWI WCCGGW 3 cut(s) 70, 1510, 1643
Bsc4I CCNNNNNNNGG 4 cut(s) 662, 744, 1769, 2141
Bse118I RCCGGY 1 cut(s) 1510
Bse1I ACTGG 2 cut(s) 1855, 2029
Bse3DI GCAATG 2 cut(s) 321, 1186
Bse8I GATNNNNATC 2 cut(s) 113, 1071
BseBI CCWGG 4 cut(s) 1491, 1764, 2217, 2289
BseDI CCNNGG 8 cut(s) 24, 404, 738, 1032, 1489, 2288, 2342, 2376
BseGI GGATG 6 cut(s) 1018, 1315, 1688, 1718, 1729, 2071
BseJI GATNNNNATC 2 cut(s) 113, 1071
BseLI CCNNNNNNNGG 4 cut(s) 662, 744, 1769, 2141
BseMI GCAATG 2 cut(s) 321, 1186
BseNI ACTGG 2 cut(s) 1855, 2029
BseRI GAGGAG 2 cut(s) 659, 1110
BseSI GKGCMC 1 cut(s) 2435
BseX3I CGGCCG 1 cut(s) 686
BseXI GCAGC 5 cut(s) 142, 1178, 1941, 1968, 2068
BsgI GTGCAG 1 cut(s) 1659
Bsh1236I CGCG 3 cut(s) 526, 979, 1952
Bsh1285I CGRYCG 2 cut(s) 689, 693
BshFI GGCC 5 cut(s) 209, 688, 796, 1031, 2433
BshTI ACCGGT 1 cut(s) 1510
BsiEI CGRYCG 2 cut(s) 689, 693
BsiHKAI GWGCWC 4 cut(s) 60, 723, 2019, 2297
BsiHKCI CYCGRG 2 cut(s) 737, 1080
BsiSI CCGG 4 cut(s) 71, 534, 1511, 1644
BslFI GGGAC 3 cut(s) 893, 1472, 1945
BslI CCNNNNNNNGG 4 cut(s) 662, 744, 1769, 2141
BsmAI GTCTC 7 cut(s) 416, 431, 1124, 1501, 1554, 1908, 2125
BsmFI GGGAC 3 cut(s) 893, 1472, 1945
BsmI GAATGC 3 cut(s) 399, 1359, 2095
BsnI GGCC 5 cut(s) 209, 688, 796, 1031, 2433
Bso31I GGTCTC 1 cut(s) 1501
BsoBI CYCGRG 2 cut(s) 737, 1080
Bsp119I TTCGAA 2 cut(s) 112, 2363
Bsp120I GGGCCC 1 cut(s) 2431
Bsp1286I GDGCHC 5 cut(s) 60, 723, 2019, 2297, 2435
Bsp19I CCATGG 1 cut(s) 1032
Bsp68I TCGCGA 1 cut(s) 526
BspACI CCGC 7 cut(s) 275, 844, 979, 2004, 2135, 2390, 2394
BspANI GGCC 5 cut(s) 209, 688, 796, 1031, 2433
BspFNI CGCG 3 cut(s) 526, 979, 1952
BspLI GGNNCC 3 cut(s) 363, 651, 2433
BspMAI CTGCAG 1 cut(s) 1986
BspMI ACCTGC 1 cut(s) 1681
BspPI GGATC 4 cut(s) 1080, 1801, 2227, 2367
BspQI GCTCTTC 2 cut(s) 2024, 2302
BspT104I TTCGAA 2 cut(s) 112, 2363
BspTNI GGTCTC 1 cut(s) 1501
BsrDI GCAATG 2 cut(s) 321, 1186
BsrFI RCCGGY 1 cut(s) 1510
BsrI ACTGG 2 cut(s) 1855, 2029
BssAI RCCGGY 1 cut(s) 1510
BssECI CCNNGG 8 cut(s) 24, 404, 738, 1032, 1489, 2288, 2342, 2376
BssNI GRCGYC 1 cut(s) 2273
BssSI CACGAG 2 cut(s) 250, 507
BssT1I CCWWGG 4 cut(s) 404, 1032, 2342, 2376
Bst2BI CACGAG 2 cut(s) 250, 507
Bst2UI CCWGG 4 cut(s) 1491, 1764, 2217, 2289
Bst4CI ACNGT 6 cut(s) 660, 953, 1477, 1696, 1839, 2305
Bst6I CTCTTC 5 cut(s) 291, 1586, 1882, 2024, 2302
BstACI GRCGYC 1 cut(s) 2273
BstBI TTCGAA 2 cut(s) 112, 2363
BstC8I GCNNGC 7 cut(s) 41, 197, 798, 842, 1177, 2331, 2392
BstDSI CCRYGG 2 cut(s) 24, 1032
BstEII GGTNACC 1 cut(s) 665
BstF5I GGATG 6 cut(s) 1018, 1315, 1688, 1718, 1729, 2071
BstFNI CGCG 3 cut(s) 526, 979, 1952
BstHHI GCGC 1 cut(s) 1954
BstMAI GTCTC 7 cut(s) 416, 431, 1124, 1501, 1554, 1908, 2125
BstMCI CGRYCG 2 cut(s) 689, 693
BstMWI GCNNNNNNNGC 6 cut(s) 45, 325, 351, 1124, 1133, 2090
BstNI CCWGG 4 cut(s) 1491, 1764, 2217, 2289
BstNSI RCATGY 2 cut(s) 1179, 1703
BstPI GGTNACC 1 cut(s) 665
BstSCI CCNGG 4 cut(s) 1489, 1762, 2215, 2287
BstSFI CTRYAG 2 cut(s) 1467, 1982
BstSLI GKGCMC 1 cut(s) 2435
BstUI CGCG 3 cut(s) 526, 979, 1952
BstV1I GCAGC 5 cut(s) 142, 1178, 1941, 1968, 2068
BstV2I GAAGAC 2 cut(s) 755, 991
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
BstZI CGGCCG 1 cut(s) 686
BsuRI GGCC 5 cut(s) 209, 688, 796, 1031, 2433
BtgI CCRYGG 2 cut(s) 24, 1032
BtsCI GGATG 6 cut(s) 1018, 1315, 1688, 1718, 1729, 2071
BtsI GCAGTG 1 cut(s) 2154
BtsIMutI CAGTG 3 cut(s) 656, 2022, 2154
BtuMI TCGCGA 1 cut(s) 526
BveI ACCTGC 1 cut(s) 1681
Cac8I GCNNGC 7 cut(s) 41, 197, 798, 842, 1177, 2331, 2392
CaiI CAGNNNCTG 1 cut(s) 1191
CfoI GCGC 1 cut(s) 1954
Cfr10I RCCGGY 1 cut(s) 1510
Cfr13I GGNCC 6 cut(s) 21, 1544, 2213, 2396, 2431, 2432
CseI GACGC 3 cut(s) 71, 846, 2262
Csp6I GTAC 6 cut(s) 864, 1322, 1608, 1670, 1697, 2262
CspAI ACCGGT 1 cut(s) 1510
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviQI GTAC 6 cut(s) 864, 1322, 1608, 1670, 1697, 2262
DraI TTTAAA 2 cut(s) 1201, 1927
DraIII CACNNNGTG 1 cut(s) 310
EaeI YGGCCR 3 cut(s) 207, 686, 1029
EagI CGGCCG 1 cut(s) 686
Eam1104I CTCTTC 5 cut(s) 291, 1586, 1882, 2024, 2302
EarI CTCTTC 5 cut(s) 291, 1586, 1882, 2024, 2302
Ecl136II GAGCTC 2 cut(s) 58, 2295
EclXI CGGCCG 1 cut(s) 686
Eco130I CCWWGG 4 cut(s) 404, 1032, 2342, 2376
Eco24I GRGCYC 3 cut(s) 60, 2297, 2435
Eco31I GGTCTC 1 cut(s) 1501
Eco32I GATATC 1 cut(s) 1302
Eco47I GGWCC 4 cut(s) 21, 1544, 2213, 2396
Eco52I CGGCCG 1 cut(s) 686
Eco53kI GAGCTC 2 cut(s) 58, 2295
Eco57I CTGAAG 3 cut(s) 1257, 1668, 2298
Eco88I CYCGRG 2 cut(s) 737, 1080
Eco91I GGTNACC 1 cut(s) 665
EcoICRI GAGCTC 2 cut(s) 58, 2295
EcoO109I RGGNCCY 2 cut(s) 2431, 2432
EcoO65I GGTNACC 1 cut(s) 665
EcoRII CCWGG 4 cut(s) 1489, 1762, 2215, 2287
EcoRV GATATC 1 cut(s) 1302
EcoT14I CCWWGG 4 cut(s) 404, 1032, 2342, 2376
EcoT22I ATGCAT 2 cut(s) 889, 1359
EcoT38I GRGCYC 3 cut(s) 60, 2297, 2435
ErhI CCWWGG 4 cut(s) 404, 1032, 2342, 2376
FalI AAGNNNNNCTT 2 cut(s) 2003, 2035
FaqI GGGAC 3 cut(s) 893, 1472, 1945
FauI CCCGC 1 cut(s) 2383
FbaI TGATCA 1 cut(s) 234
Fnu4HI GCNGC 6 cut(s) 131, 1192, 1955, 1982, 2005, 2082
FokI GGATG 6 cut(s) 1005, 1322, 1675, 1725, 1736, 2078
FriOI GRGCYC 3 cut(s) 60, 2297, 2435
Fsp4HI GCNGC 6 cut(s) 131, 1192, 1955, 1982, 2005, 2082
FspBI CTAG 5 cut(s) 908, 1533, 1622, 1665, 2387
GlaI GCGC 1 cut(s) 1953
GluI GCNGC 6 cut(s) 131, 1192, 1955, 1982, 2005, 2082
GsuI CTGGAG 1 cut(s) 2163
HaeIII GGCC 5 cut(s) 209, 688, 796, 1031, 2433
HapII CCGG 4 cut(s) 71, 534, 1511, 1644
HgaI GACGC 3 cut(s) 71, 846, 2262
HhaI GCGC 1 cut(s) 1954
Hin1I GRCGYC 1 cut(s) 2273
Hin6I GCGC 1 cut(s) 1952
HinP1I GCGC 1 cut(s) 1952
HpaII CCGG 4 cut(s) 71, 534, 1511, 1644
HphI GGTGA 5 cut(s) 65, 85, 1376, 1658, 2294
Hpy166II GTNNAC 7 cut(s) 140, 149, 1649, 1856, 1948, 2152, 2399
Hpy8I GTNNAC 7 cut(s) 140, 149, 1649, 1856, 1948, 2152, 2399
Hpy99I CGWCG 4 cut(s) 65, 437, 526, 687
HpyAV CCTTC 6 cut(s) 911, 1284, 1343, 2046, 2273, 2422
HpyCH4III ACNGT 6 cut(s) 660, 953, 1477, 1696, 1839, 2305
HpyCH4IV ACGT 3 cut(s) 521, 556, 1013
HpyF10VI GCNNNNNNNGC 6 cut(s) 45, 325, 351, 1124, 1133, 2090
HpySE526I ACGT 3 cut(s) 521, 556, 1013
Hsp92I GRCGYC 1 cut(s) 2273
HspAI GCGC 1 cut(s) 1952
Ksp22I TGATCA 1 cut(s) 234
LguI GCTCTTC 2 cut(s) 2024, 2302
LmnI GCTCC 6 cut(s) 1123, 1342, 1646, 1849, 2144, 2292
Lsp1109I GCAGC 5 cut(s) 142, 1178, 1941, 1968, 2068
LweI GCATC 7 cut(s) 182, 738, 1300, 1697, 1925, 1991, 2056
MaeI CTAG 5 cut(s) 908, 1533, 1622, 1665, 2387
MaeII ACGT 3 cut(s) 521, 556, 1013
MaeIII GTNAC 4 cut(s) 73, 557, 665, 789
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 5 cut(s) 60, 723, 2019, 2297, 2435
MlsI TGGCCA 1 cut(s) 1031
MluNI TGGCCA 1 cut(s) 1031
MlyI GAGTC 6 cut(s) 433, 745, 1406, 1642, 1787, 2320
MmeI TCCRAC 2 cut(s) 1027, 1871
Mox20I TGGCCA 1 cut(s) 1031
Mph1103I ATGCAT 2 cut(s) 889, 1359
MroXI GAANNNNTTC 2 cut(s) 300, 1231
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 7 cut(s) 824, 1200, 1442, 1748, 1790, 1830, 1926
MslI CAYNNNNRTG 1 cut(s) 1362
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 1 cut(s) 2225
MspI CCGG 4 cut(s) 71, 534, 1511, 1644
MspR9I CCNGG 4 cut(s) 1491, 1764, 2217, 2289
MunI CAATTG 1 cut(s) 417
Mva1269I GAATGC 3 cut(s) 399, 1359, 2095
MvaI CCWGG 4 cut(s) 1491, 1764, 2217, 2289
MvnI CGCG 3 cut(s) 526, 979, 1952
MwoI GCNNNNNNNGC 6 cut(s) 45, 325, 351, 1124, 1133, 2090
NcoI CCATGG 1 cut(s) 1032
NlaIV GGNNCC 3 cut(s) 363, 651, 2433
NmeAIII GCCGAG 1 cut(s) 2398
NmuCI GTSAC 1 cut(s) 73
NruI TCGCGA 1 cut(s) 526
NsiI ATGCAT 2 cut(s) 889, 1359
NspI RCATGY 2 cut(s) 1179, 1703
NspV TTCGAA 2 cut(s) 112, 2363
PaeI GCATGC 1 cut(s) 1179
PaeR7I CTCGAG 1 cut(s) 1080
PciSI GCTCTTC 2 cut(s) 2024, 2302
PctI GAATGC 3 cut(s) 399, 1359, 2095
PdmI GAANNNNTTC 2 cut(s) 300, 1231
PfoI TCCNGGA 1 cut(s) 2215
PinAI ACCGGT 1 cut(s) 1510
PkrI GCNGC 6 cut(s) 132, 1193, 1956, 1983, 2006, 2083
Ple19I CGATCG 1 cut(s) 693
PleI GAGTC 6 cut(s) 433, 745, 1406, 1641, 1786, 2319
PpsI GAGTC 6 cut(s) 433, 745, 1406, 1641, 1786, 2319
Psp124BI GAGCTC 2 cut(s) 60, 2297
Psp1406I AACGTT 1 cut(s) 556
Psp6I CCWGG 4 cut(s) 1489, 1762, 2215, 2287
PspEI GGTNACC 1 cut(s) 665
PspGI CCWGG 4 cut(s) 1489, 1762, 2215, 2287
PspN4I GGNNCC 3 cut(s) 363, 651, 2433
PspOMI GGGCCC 1 cut(s) 2431
PspPI GGNCC 6 cut(s) 21, 1544, 2213, 2396, 2431, 2432
PspXI VCTCGAGB 1 cut(s) 1080
PstI CTGCAG 1 cut(s) 1986
PstNI CAGNNNCTG 1 cut(s) 1191
PsuI RGATCY 1 cut(s) 808
PvuI CGATCG 1 cut(s) 693
PvuII CAGCTG 1 cut(s) 2225
RruI TCGCGA 1 cut(s) 526
RsaI GTAC 6 cut(s) 865, 1323, 1609, 1671, 1698, 2263
RsaNI GTAC 6 cut(s) 864, 1322, 1608, 1670, 1697, 2262
RseI CAYNNNNRTG 1 cut(s) 1362
SacI GAGCTC 2 cut(s) 60, 2297
SapI GCTCTTC 2 cut(s) 2024, 2302
SaqAI TTAA 7 cut(s) 824, 1200, 1442, 1748, 1790, 1830, 1926
SatI GCNGC 6 cut(s) 131, 1192, 1955, 1982, 2005, 2082
Sau96I GGNCC 6 cut(s) 21, 1544, 2213, 2396, 2431, 2432
ScaI AGTACT 1 cut(s) 2263
SchI GAGTC 6 cut(s) 433, 745, 1406, 1642, 1787, 2320
ScrFI CCNGG 4 cut(s) 1491, 1764, 2217, 2289
SduI GDGCHC 5 cut(s) 60, 723, 2019, 2297, 2435
SfaNI GCATC 7 cut(s) 182, 738, 1300, 1697, 1925, 1991, 2056
SfcI CTRYAG 2 cut(s) 1467, 1982
Sfr274I CTCGAG 1 cut(s) 1080
SfuI TTCGAA 2 cut(s) 112, 2363
SinI GGWCC 4 cut(s) 21, 1544, 2213, 2396
SlaI CTCGAG 1 cut(s) 1080
SmiMI CAYNNNNRTG 1 cut(s) 1362
SmlI CTYRAG 3 cut(s) 266, 1080, 1122
SmoI CTYRAG 3 cut(s) 266, 1080, 1122
SphI GCATGC 1 cut(s) 1179
SsiI CCGC 7 cut(s) 275, 844, 979, 2004, 2135, 2390, 2394
SspMI CTAG 5 cut(s) 908, 1533, 1622, 1665, 2387
SstI GAGCTC 2 cut(s) 60, 2297
StyD4I CCNGG 4 cut(s) 1489, 1762, 2215, 2287
StyI CCWWGG 4 cut(s) 404, 1032, 2342, 2376
TaaI ACNGT 6 cut(s) 660, 953, 1477, 1696, 1839, 2305
TaiI ACGT 3 cut(s) 524, 559, 1016
TaqI TCGA 8 cut(s) 60, 112, 201, 432, 1081, 1209, 1756, 2363
TatI WGTACW 2 cut(s) 1696, 2261
TauI GCSGC 1 cut(s) 2007
Tru1I TTAA 7 cut(s) 824, 1200, 1442, 1748, 1790, 1830, 1926
Tru9I TTAA 7 cut(s) 824, 1200, 1442, 1748, 1790, 1830, 1926
TscAI CASTG 3 cut(s) 663, 2029, 2154
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 5 cut(s) 130, 1191, 1954, 1981, 2081
Tsp45I GTSAC 1 cut(s) 73
TspRI CASTG 3 cut(s) 663, 2029, 2154
VpaK11BI GGWCC 4 cut(s) 21, 1544, 2213, 2396
XapI RAATTY 4 cut(s) 86, 1230, 1264, 1657
XbaI TCTAGA 2 cut(s) 907, 1664
XceI RCATGY 2 cut(s) 1179, 1703
XhoI CTCGAG 1 cut(s) 1080
XmnI GAANNNNTTC 2 cut(s) 300, 1231
XspI CTAG 5 cut(s) 908, 1533, 1622, 1665, 2387
ZrmI AGTACT 1 cut(s) 2263
Zsp2I ATGCAT 2 cut(s) 889, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.