Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0002842

Physical Location & Seq

Genomic Coordinates Forward (+)

10419168 .. 10426149

Gene Structure 6982 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG128000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2376 bp

ATGAGCAGTTTAGGGTTTAGGTCCGTGGTCTATCACGGCGAGCTGTGCTTGGGTGAGCTCGACGCTATTCCGGTGACGGAGGGGAAATTTCAGTTCCCGAACAACGAGATTCGAATCCATCACATATCGCAGCAAAGTGAACGCTGTCCACCCCTCTCAGTTCTCCAAACGATTTCCTCATTTTCGGTTAGATGCAAGCTCGAATCGGCCACCCCTGTTGAACAGCCTCATTTGATCAACCTCCACGCCTCCTGTTTCTACGAGCTCAAGACGGCGGTAGTGTTGGTTGGGAATGAAGAGATTCACTTGGTGGCAATGCCAAGTAAGCAGAAAAAGTTTCCGTGCTTTTGGTGCTATTCGGTTCCTTTAGGTCTGTATAATGCTTCTCTTGGAATGCTGAACCCAAGGTGTCTCTCAATTGTATTTGATCTCGACGAGACTCTAATTGTTGCCAACACAATGAAGTCGTTTGAGGATAGAATTGAGGCTTTACGAAGCTGGATTGCACGAGAGAGTGAAACGTCGCGAATAGCCGGAATGTCTAATGAAATGAAACGTTACATTGACGATAGGTGGTTGTTGAGGCAGTATATAGATAATGATTGTGTGGTGGATAACGGACAGGTGTATAAGATTCAAATGGAGGAGGTTCCTCCACTGTCAGGTAACCACGAGAGAATCCGTCGGCCCATCGTTAGGTTGCCAGACAGGAACATCGTGCTCACTCGCATCAATCCCGAGGTTCGTGATACAAGTGTGCTGGTCAGGTTACGGCCTGCGTGGGAAGATCTGAGAAGTTATTTAACAGCAAAAGGACGCAAGCGGTTTGAAGTTCATGTCTGTACGATGGCTGAAAGGGACTATGCATTAGAAATGTGGAGGCTTCTAGACCCAGAAGGACAACTAATAGGTTCAAAGCAACTTCTTGACCGTGTTGTTTGTGTCAAATCAGGTTCGCGGAAATCTTTACTCAATGTCTTCCAACTTGGAACGTGCCATCCAAAGATGGCCATGGTTATTGATGATCGTTCAAAGGTCTGGGAAGATAAGGATCAACCTCGAGTTCATGTAGTTCCAGCTTTTACTCCTTATTATGCTCCTCAAGCAGAGACAGCAAATGCCGTTCCTGTCCTCTGTGTAGCAAGAAATGTTGCATGCAATGTCAGGGGCTGCTTTTTTAAAGAGTTCGATGAAATTTTCTTGCGAAGAATTTCTGAAGTTTTTTATGAAGATGAGGTGGTAAATTTACCTCCTTCTCCTGATGTGAGCAATTACTTGATATCAGAGGATGCTGGTTTTGTACCAAATGGAATTGCTCCCTTTAGTGAAGGAATGCATGGTGGTGAAGTTGAACGGAGATTGAACCAACCTGATGATAAGGGTGTTATGGACTCAATGGCTTATTCTAATCATGCTGAGTTAAGACCTGACAACTCCCAGAAACCTGTAGCAACTGTCCTCAATGTCCCTGGTGCAACATCTTTGAGACCGGTCATGCCTACTCAGAAACCTAGTTTGCTTGGACCTCTTGTTAGACGAGACAGCTTTTCTGATCGTGATAATAACATGAAGAGAGGACTTCTTGGTACAAATCCTTGCCTAGATATAAGGAGTCAAAGCTCTGGTGAACCCCCAAATTCATCTAGAGTACCTGCACAAACTTCAGCATCCACAGTACATGCACAAGGAGGATGGTTGGTGGATGATGACAATAAAAGAGGACATTTAAGTTTTCGACCTCCTGTTTCTGGTCAAGAGTCTGATGTCTTAAAACTTGAGAAGCTGATTCACCAGAATCCGTTTAGTCTTAATAAACAGTCTGCTCCGACTGGTTTACTCTCTCAGACATCTGAGGCAAGGAGGGAAGAGGTTAGCTCTGGACATGATTTCCCAAAGCAAAATCTTCCTTTTCCAAGTGTGTTATCAGAGGCTGGTGTGTCTCAGAATCAGGCATCTTCTTTTAAAAAAGAATCTGGAAAAGTGAACGCGCTGCCGTCCCCTTTATCTATTGGTGTGCTGCAGGAAATCGGACGGAGATGCGGCTCAAAGGTTGAATTTAAGGCTGTTATAAGCACCAGTAACGATTTGCAGTTTTCTTTTGAGGTGCTCTTCACTGGGGAGAAGATTGGTTTTGGAACAGGAAGGACAAGAAAGGATGCTCAACAACAGGCTGCTGAGAATGCACTTCACAGCTTGGCTGAAAAATATGTCTCATATATTGCACCGCAATCTGGAGCAGTGGACAAAGATTTTGATAAACTCTCTCTTGGAAATGAAAACGGATTTGTTTTGGACATAGTTGGTCCAGGATCAGCTGAGGGGCTAACGGAAGATGGATTGCCAAAAGAAAGTACTTCTGAGGTGGGGATTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF dsrm

Protein Analysis

791

Amino Acids

88.04

Weight (kDa)

6.16

Isoelectric Point (pI)

54.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	245 - 356	1.1e-07	NLI interacting factor-like phosphatase
dsrm	PF00035	670 - 732	2.2e-08	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002842)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01270 AT5G01270
fragaria_vesca	FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640 FvH4_6g11640
malus_domestica	MD04G1150600.v1.1 MD04G1150700.v1.1 MD12G1164700.v1.1
prunus_persica	Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1 Prupe.6G275400_v2.0.a1
pyrus_communis	pycom04g13630 pycom12g15760
rosa_chinensis	RchiOBHm_Chr3g0463301
rosa_laevigata	RLG00000024792
rosa_multiflora	Rmu_co8080032.1_g000001 Rmu_sc0000923.1_g000001 Rmu_sc0002976.1_g000008 Rmu_sc0033161.1_g000001
rosa_roxburghii	Rroxscaffold_6G00416690
rosa_rugosa	Rorug03G0063700 Rorug03G0063700
rosa_samantha	Rh3AG121600 Rh3BG126100 Rh3CG128000 Rh3DG127200
rosa_wichuraiana	Rw3G010330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2069
Acc36I	ACCTGC	1 cut(s)	1660
AccII	CGCG	3 cut(s)	526, 958, 1988
AciI	CCGC	5 cut(s)	275, 823, 958, 2040, 2225
AclI	AACGTT	1 cut(s)	556
AclWI	GGATC	2 cut(s)	1059, 2317
AcoI	YGGCCR	2 cut(s)	207, 1008
AcsI	RAATTY	6 cut(s)	86, 1194, 1209, 1243, 1636, 2054
AcuI	CTGAAG	2 cut(s)	1236, 1647
AdeI	CACNNNGTG	1 cut(s)	310
AfaI	GTAC	6 cut(s)	844, 1302, 1588, 1650, 1677, 2353
AfiI	CCNNNNNNNGG	4 cut(s)	662, 696, 1748, 2231
AgeI	ACCGGT	1 cut(s)	1489
AgsI	TTSAA	8 cut(s)	221, 638, 830, 915, 1032, 1352, 1363, 2054
AjnI	CCWGG	2 cut(s)	1468, 2305
AloI	GAACNNNNNNTCC	2 cut(s)	982, 1014
Alw21I	GWGCWC	4 cut(s)	60, 267, 723, 2109
Alw26I	GTCTC	7 cut(s)	416, 431, 1103, 1480, 1533, 1944, 2215
AlwI	GGATC	2 cut(s)	1059, 2317
AlwNI	CAGNNNCTG	2 cut(s)	1170, 1931
Ama87I	CYCGRG	2 cut(s)	737, 1059
AoxI	GGCC	4 cut(s)	207, 686, 773, 1008
ApeKI	GCWGC	5 cut(s)	130, 1170, 1990, 2017, 2171
ApoI	RAATTY	6 cut(s)	86, 1194, 1209, 1243, 1636, 2054
ArsI	GACNNNNNNTTYG	2 cut(s)	2234, 2266
AsiGI	ACCGGT	1 cut(s)	1489
Asp700I	GAANNNNTTC	2 cut(s)	300, 1210
AspLEI	GCGC	1 cut(s)	1990
AspS9I	GGNCC	4 cut(s)	21, 687, 1523, 2303
AsuHPI	GGTGA	5 cut(s)	65, 85, 1355, 1637, 1781
AsuII	TTCGAA	1 cut(s)	112
AvaI	CYCGRG	2 cut(s)	737, 1059
AvaII	GGWCC	3 cut(s)	21, 1523, 2303
BaeI	ACNNNNGTAYC	2 cut(s)	741, 774
BalI	TGGCCA	1 cut(s)	1010
BanII	GRGCYC	2 cut(s)	60, 267
BauI	CACGAG	2 cut(s)	507, 671
BbsI	GAAGAC	1 cut(s)	970
Bbv12I	GWGCWC	4 cut(s)	60, 267, 723, 2109
BbvCI	CCTCAGC	1 cut(s)	2316
BbvI	GCAGC	5 cut(s)	142, 1157, 1977, 2004, 2158
BccI	CCATC	7 cut(s)	126, 698, 841, 1000, 1005, 1686, 2327
BceAI	ACGGC	5 cut(s)	52, 288, 788, 1106, 1978
BciT130I	CCWGG	2 cut(s)	1470, 2307
BclI	TGATCA	1 cut(s)	234
BcoDI	GTCTC	7 cut(s)	416, 431, 1103, 1480, 1533, 1944, 2215
BfaI	CTAG	4 cut(s)	887, 1512, 1601, 1644
BfmI	CTRYAG	2 cut(s)	1446, 2018
BfuAI	ACCTGC	1 cut(s)	1660
BglII	AGATCT	1 cut(s)	787
BisI	GCNGC	6 cut(s)	131, 1171, 1991, 2018, 2041, 2172
BlsI	GCNGC	6 cut(s)	132, 1172, 1992, 2019, 2042, 2173
BmcAI	AGTACT	1 cut(s)	2353
Bme1390I	CCNGG	2 cut(s)	1470, 2307
Bme18I	GGWCC	3 cut(s)	21, 1523, 2303
BmeT110I	CYCGRG	2 cut(s)	737, 1059
BmgT120I	GGNCC	4 cut(s)	21, 687, 1523, 2303
BmiI	GGNNCC	2 cut(s)	363, 651
BmrFI	CCNGG	2 cut(s)	1470, 2307
BmrI	ACTGGG	1 cut(s)	2124
BmsI	GCATC	7 cut(s)	182, 738, 1279, 1676, 1961, 2027, 2146
BmuI	ACTGGG	1 cut(s)	2124
BpiI	GAAGAC	1 cut(s)	970
BplI	GAGNNNNNCTC	2 cut(s)	1859, 1891
BpmI	CTGGAG	1 cut(s)	2253
Bpu10I	CCTNAGC	1 cut(s)	2316
Bpu14I	TTCGAA	1 cut(s)	112
BpuEI	CTTGAG	3 cut(s)	251, 1086, 1796
BsaBI	GATNNNNATC	2 cut(s)	113, 1050
BsaI	GGTCTC	1 cut(s)	1480
BsaJI	CCNNGG	5 cut(s)	24, 404, 738, 1011, 1468
BsaWI	WCCGGW	2 cut(s)	70, 1489
Bsc4I	CCNNNNNNNGG	4 cut(s)	662, 696, 1748, 2231
Bse118I	RCCGGY	1 cut(s)	1489
Bse1I	ACTGG	3 cut(s)	1834, 2076, 2119
Bse3DI	GCAATG	2 cut(s)	321, 1165
Bse8I	GATNNNNATC	2 cut(s)	113, 1050
BseBI	CCWGG	2 cut(s)	1470, 2307
BseDI	CCNNGG	5 cut(s)	24, 404, 738, 1011, 1468
BseGI	GGATG	6 cut(s)	997, 1294, 1667, 1697, 1708, 2161
BseJI	GATNNNNATC	2 cut(s)	113, 1050
BseLI	CCNNNNNNNGG	4 cut(s)	662, 696, 1748, 2231
BseMI	GCAATG	2 cut(s)	321, 1165
BseNI	ACTGG	3 cut(s)	1834, 2076, 2119
BseRI	GAGGAG	2 cut(s)	659, 1089
BseXI	GCAGC	5 cut(s)	142, 1157, 1977, 2004, 2158
BsgI	GTGCAG	1 cut(s)	1638
Bsh1236I	CGCG	3 cut(s)	526, 958, 1988
BshFI	GGCC	4 cut(s)	209, 688, 775, 1010
BshTI	ACCGGT	1 cut(s)	1489
BsiHKAI	GWGCWC	4 cut(s)	60, 267, 723, 2109
BsiHKCI	CYCGRG	2 cut(s)	737, 1059
BsiSI	CCGG	3 cut(s)	71, 534, 1490
BslFI	GGGAC	3 cut(s)	872, 1451, 1981
BslI	CCNNNNNNNGG	4 cut(s)	662, 696, 1748, 2231
BsmAI	GTCTC	7 cut(s)	416, 431, 1103, 1480, 1533, 1944, 2215
BsmFI	GGGAC	3 cut(s)	872, 1451, 1981
BsmI	GAATGC	3 cut(s)	399, 1338, 2185
BsnI	GGCC	4 cut(s)	209, 688, 775, 1010
Bso31I	GGTCTC	1 cut(s)	1480
BsoBI	CYCGRG	2 cut(s)	737, 1059
Bsp119I	TTCGAA	1 cut(s)	112
Bsp1286I	GDGCHC	4 cut(s)	60, 267, 723, 2109
Bsp143I	GATC	7 cut(s)	234, 427, 787, 1024, 1051, 1552, 2309
Bsp19I	CCATGG	1 cut(s)	1011
Bsp68I	TCGCGA	1 cut(s)	526
BspACI	CCGC	5 cut(s)	275, 823, 958, 2040, 2225
BspANI	GGCC	4 cut(s)	209, 688, 775, 1010
BspFNI	CGCG	3 cut(s)	526, 958, 1988
BspLI	GGNNCC	2 cut(s)	363, 651
BspMAI	CTGCAG	1 cut(s)	2022
BspMI	ACCTGC	1 cut(s)	1660
BspPI	GGATC	2 cut(s)	1059, 2317
BspQI	GCTCTTC	1 cut(s)	2114
BspT104I	TTCGAA	1 cut(s)	112
BspTNI	GGTCTC	1 cut(s)	1480
BsrDI	GCAATG	2 cut(s)	321, 1165
BsrFI	RCCGGY	1 cut(s)	1489
BsrI	ACTGG	3 cut(s)	1834, 2076, 2119
BssAI	RCCGGY	1 cut(s)	1489
BssECI	CCNNGG	5 cut(s)	24, 404, 738, 1011, 1468
BssMI	GATC	7 cut(s)	234, 427, 787, 1024, 1051, 1552, 2309
BssSI	CACGAG	2 cut(s)	507, 671
BssT1I	CCWWGG	2 cut(s)	404, 1011
Bst2BI	CACGAG	2 cut(s)	507, 671
Bst2UI	CCWGG	2 cut(s)	1470, 2307
Bst4CI	ACNGT	5 cut(s)	660, 932, 1456, 1675, 1818
Bst6I	CTCTTC	4 cut(s)	291, 1565, 1860, 2114
BstBI	TTCGAA	1 cut(s)	112
BstC8I	GCNNGC	5 cut(s)	41, 197, 777, 821, 1156
BstDSI	CCRYGG	2 cut(s)	24, 1011
BstEII	GGTNACC	1 cut(s)	665
BstF5I	GGATG	6 cut(s)	997, 1294, 1667, 1697, 1708, 2161
BstFNI	CGCG	3 cut(s)	526, 958, 1988
BstHHI	GCGC	1 cut(s)	1990
BstKTI	GATC	7 cut(s)	237, 430, 790, 1027, 1054, 1555, 2312
BstMAI	GTCTC	7 cut(s)	416, 431, 1103, 1480, 1533, 1944, 2215
BstMBI	GATC	7 cut(s)	234, 427, 787, 1024, 1051, 1552, 2309
BstMWI	GCNNNNNNNGC	6 cut(s)	45, 325, 351, 1103, 1112, 2180
BstNI	CCWGG	2 cut(s)	1470, 2307
BstNSI	RCATGY	2 cut(s)	1158, 1682
BstPI	GGTNACC	1 cut(s)	665
BstSCI	CCNGG	2 cut(s)	1468, 2305
BstSFI	CTRYAG	2 cut(s)	1446, 2018
BstUI	CGCG	3 cut(s)	526, 958, 1988
BstV1I	GCAGC	5 cut(s)	142, 1157, 1977, 2004, 2158
BstV2I	GAAGAC	1 cut(s)	970
BstX2I	RGATCY	1 cut(s)	787
BstYI	RGATCY	1 cut(s)	787
BsuRI	GGCC	4 cut(s)	209, 688, 775, 1010
BtgI	CCRYGG	2 cut(s)	24, 1011
BtsCI	GGATG	6 cut(s)	997, 1294, 1667, 1697, 1708, 2161
BtsI	GCAGTG	1 cut(s)	2244
BtsIMutI	CAGTG	3 cut(s)	656, 2112, 2244
BtuMI	TCGCGA	1 cut(s)	526
BveI	ACCTGC	1 cut(s)	1660
Cac8I	GCNNGC	5 cut(s)	41, 197, 777, 821, 1156
CaiI	CAGNNNCTG	2 cut(s)	1170, 1931
CfoI	GCGC	1 cut(s)	1990
Cfr10I	RCCGGY	1 cut(s)	1489
Cfr13I	GGNCC	4 cut(s)	21, 687, 1523, 2303
CseI	GACGC	2 cut(s)	71, 825
Csp6I	GTAC	6 cut(s)	843, 1301, 1587, 1649, 1676, 2352
CspAI	ACCGGT	1 cut(s)	1489
CspCI	CAANNNNNGTGG	2 cut(s)	199, 234
CviQI	GTAC	6 cut(s)	843, 1301, 1587, 1649, 1676, 2352
DpnI	GATC	7 cut(s)	236, 429, 789, 1026, 1053, 1554, 2311
DpnII	GATC	7 cut(s)	234, 427, 787, 1024, 1051, 1552, 2309
DraI	TTTAAA	2 cut(s)	1180, 1963
DraIII	CACNNNGTG	1 cut(s)	310
EaeI	YGGCCR	2 cut(s)	207, 1008
Eam1104I	CTCTTC	4 cut(s)	291, 1565, 1860, 2114
EarI	CTCTTC	4 cut(s)	291, 1565, 1860, 2114
Ecl136II	GAGCTC	2 cut(s)	58, 265
Eco130I	CCWWGG	2 cut(s)	404, 1011
Eco24I	GRGCYC	2 cut(s)	60, 267
Eco31I	GGTCTC	1 cut(s)	1480
Eco32I	GATATC	1 cut(s)	1281
Eco47I	GGWCC	3 cut(s)	21, 1523, 2303
Eco53kI	GAGCTC	2 cut(s)	58, 265
Eco57I	CTGAAG	2 cut(s)	1236, 1647
Eco88I	CYCGRG	2 cut(s)	737, 1059
Eco91I	GGTNACC	1 cut(s)	665
EcoICRI	GAGCTC	2 cut(s)	58, 265
EcoO65I	GGTNACC	1 cut(s)	665
EcoRII	CCWGG	2 cut(s)	1468, 2305
EcoRV	GATATC	1 cut(s)	1281
EcoT14I	CCWWGG	2 cut(s)	404, 1011
EcoT22I	ATGCAT	2 cut(s)	868, 1338
EcoT38I	GRGCYC	2 cut(s)	60, 267
ErhI	CCWWGG	2 cut(s)	404, 1011
FaqI	GGGAC	3 cut(s)	872, 1451, 1981
FbaI	TGATCA	1 cut(s)	234
Fnu4HI	GCNGC	6 cut(s)	131, 1171, 1991, 2018, 2041, 2172
FokI	GGATG	6 cut(s)	984, 1301, 1654, 1704, 1715, 2168
FriOI	GRGCYC	2 cut(s)	60, 267
Fsp4HI	GCNGC	6 cut(s)	131, 1171, 1991, 2018, 2041, 2172
FspBI	CTAG	4 cut(s)	887, 1512, 1601, 1644
GlaI	GCGC	1 cut(s)	1989
GluI	GCNGC	6 cut(s)	131, 1171, 1991, 2018, 2041, 2172
GsuI	CTGGAG	1 cut(s)	2253
HaeIII	GGCC	4 cut(s)	209, 688, 775, 1010
HapII	CCGG	3 cut(s)	71, 534, 1490
HgaI	GACGC	2 cut(s)	71, 825
HhaI	GCGC	1 cut(s)	1990
Hin6I	GCGC	1 cut(s)	1988
HinP1I	GCGC	1 cut(s)	1988
HpaII	CCGG	3 cut(s)	71, 534, 1490
HphI	GGTGA	5 cut(s)	65, 85, 1355, 1637, 1781
Hpy166II	GTNNAC	6 cut(s)	140, 149, 1628, 1835, 1984, 2242
Hpy8I	GTNNAC	6 cut(s)	140, 149, 1628, 1835, 1984, 2242
Hpy99I	CGWCG	4 cut(s)	65, 437, 526, 687
HpyAV	CCTTC	4 cut(s)	890, 1263, 1322, 2136
HpyCH4III	ACNGT	5 cut(s)	660, 932, 1456, 1675, 1818
HpyCH4IV	ACGT	3 cut(s)	521, 556, 992
HpyF10VI	GCNNNNNNNGC	6 cut(s)	45, 325, 351, 1103, 1112, 2180
HpySE526I	ACGT	3 cut(s)	521, 556, 992
HspAI	GCGC	1 cut(s)	1988
Ksp22I	TGATCA	1 cut(s)	234
Kzo9I	GATC	7 cut(s)	234, 427, 787, 1024, 1051, 1552, 2309
LguI	GCTCTTC	1 cut(s)	2114
LmnI	GCTCC	4 cut(s)	1102, 1321, 1828, 2234
Lsp1109I	GCAGC	5 cut(s)	142, 1157, 1977, 2004, 2158
LweI	GCATC	7 cut(s)	182, 738, 1279, 1676, 1961, 2027, 2146
MaeI	CTAG	4 cut(s)	887, 1512, 1601, 1644
MaeII	ACGT	3 cut(s)	521, 556, 992
MaeIII	GTNAC	5 cut(s)	73, 557, 665, 768, 2078
MalI	GATC	7 cut(s)	236, 429, 789, 1026, 1053, 1554, 2311
MboI	GATC	7 cut(s)	234, 427, 787, 1024, 1051, 1552, 2309
MfeI	CAATTG	1 cut(s)	417
MflI	RGATCY	1 cut(s)	787
MhlI	GDGCHC	4 cut(s)	60, 267, 723, 2109
MlsI	TGGCCA	1 cut(s)	1010
MluNI	TGGCCA	1 cut(s)	1010
MlyI	GAGTC	4 cut(s)	433, 1385, 1621, 1766
MmeI	TCCRAC	2 cut(s)	1006, 1850
Mox20I	TGGCCA	1 cut(s)	1010
Mph1103I	ATGCAT	2 cut(s)	868, 1338
MroXI	GAANNNNTTC	2 cut(s)	300, 1210
MscI	TGGCCA	1 cut(s)	1010
MseI	TTAA	8 cut(s)	803, 1179, 1421, 1727, 1769, 1809, 1962, 2058
MslI	CAYNNNNRTG	1 cut(s)	1341
Msp20I	TGGCCA	1 cut(s)	1010
MspA1I	CMGCKG	1 cut(s)	2315
MspI	CCGG	3 cut(s)	71, 534, 1490
MspR9I	CCNGG	2 cut(s)	1470, 2307
MunI	CAATTG	1 cut(s)	417
Mva1269I	GAATGC	3 cut(s)	399, 1338, 2185
MvaI	CCWGG	2 cut(s)	1470, 2307
MvnI	CGCG	3 cut(s)	526, 958, 1988
MwoI	GCNNNNNNNGC	6 cut(s)	45, 325, 351, 1103, 1112, 2180
NcoI	CCATGG	1 cut(s)	1011
NdeII	GATC	7 cut(s)	234, 427, 787, 1024, 1051, 1552, 2309
NlaIV	GGNNCC	2 cut(s)	363, 651
NmuCI	GTSAC	1 cut(s)	73
NruI	TCGCGA	1 cut(s)	526
NsiI	ATGCAT	2 cut(s)	868, 1338
NspI	RCATGY	2 cut(s)	1158, 1682
NspV	TTCGAA	1 cut(s)	112
PaeI	GCATGC	1 cut(s)	1158
PaeR7I	CTCGAG	1 cut(s)	1059
PciSI	GCTCTTC	1 cut(s)	2114
PctI	GAATGC	3 cut(s)	399, 1338, 2185
PdmI	GAANNNNTTC	2 cut(s)	300, 1210
PfoI	TCCNGGA	1 cut(s)	2305
PinAI	ACCGGT	1 cut(s)	1489
PkrI	GCNGC	6 cut(s)	132, 1172, 1992, 2019, 2042, 2173
PleI	GAGTC	4 cut(s)	433, 1385, 1620, 1765
PpsI	GAGTC	4 cut(s)	433, 1385, 1620, 1765
PsiI	TTATAA	1 cut(s)	2069
Psp124BI	GAGCTC	2 cut(s)	60, 267
Psp1406I	AACGTT	1 cut(s)	556
Psp6I	CCWGG	2 cut(s)	1468, 2305
PspEI	GGTNACC	1 cut(s)	665
PspGI	CCWGG	2 cut(s)	1468, 2305
PspN4I	GGNNCC	2 cut(s)	363, 651
PspPI	GGNCC	4 cut(s)	21, 687, 1523, 2303
PspXI	VCTCGAGB	1 cut(s)	1059
PstI	CTGCAG	1 cut(s)	2022
PstNI	CAGNNNCTG	2 cut(s)	1170, 1931
PsuI	RGATCY	1 cut(s)	787
PvuII	CAGCTG	1 cut(s)	2315
RruI	TCGCGA	1 cut(s)	526
RsaI	GTAC	6 cut(s)	844, 1302, 1588, 1650, 1677, 2353
RsaNI	GTAC	6 cut(s)	843, 1301, 1587, 1649, 1676, 2352
RseI	CAYNNNNRTG	1 cut(s)	1341
SacI	GAGCTC	2 cut(s)	60, 267
SapI	GCTCTTC	1 cut(s)	2114
SaqAI	TTAA	8 cut(s)	803, 1179, 1421, 1727, 1769, 1809, 1962, 2058
SatI	GCNGC	6 cut(s)	131, 1171, 1991, 2018, 2041, 2172
Sau3AI	GATC	7 cut(s)	234, 427, 787, 1024, 1051, 1552, 2309
Sau96I	GGNCC	4 cut(s)	21, 687, 1523, 2303
ScaI	AGTACT	1 cut(s)	2353
SchI	GAGTC	4 cut(s)	433, 1385, 1621, 1766
ScrFI	CCNGG	2 cut(s)	1470, 2307
SduI	GDGCHC	4 cut(s)	60, 267, 723, 2109
SfaNI	GCATC	7 cut(s)	182, 738, 1279, 1676, 1961, 2027, 2146
SfcI	CTRYAG	2 cut(s)	1446, 2018
Sfr274I	CTCGAG	1 cut(s)	1059
SfuI	TTCGAA	1 cut(s)	112
SinI	GGWCC	3 cut(s)	21, 1523, 2303
SlaI	CTCGAG	1 cut(s)	1059
SmiMI	CAYNNNNRTG	1 cut(s)	1341
SmlI	CTYRAG	4 cut(s)	266, 1059, 1101, 1775
SmoI	CTYRAG	4 cut(s)	266, 1059, 1101, 1775
SphI	GCATGC	1 cut(s)	1158
SsiI	CCGC	5 cut(s)	275, 823, 958, 2040, 2225
SspMI	CTAG	4 cut(s)	887, 1512, 1601, 1644
SstI	GAGCTC	2 cut(s)	60, 267
StyD4I	CCNGG	2 cut(s)	1468, 2305
StyI	CCWWGG	2 cut(s)	404, 1011
TaaI	ACNGT	5 cut(s)	660, 932, 1456, 1675, 1818
TaiI	ACGT	3 cut(s)	524, 559, 995
TaqI	TCGA	8 cut(s)	60, 112, 201, 432, 1060, 1188, 1735, 2371
TatI	WGTACW	2 cut(s)	1675, 2351
TauI	GCSGC	1 cut(s)	2043
Tru1I	TTAA	8 cut(s)	803, 1179, 1421, 1727, 1769, 1809, 1962, 2058
Tru9I	TTAA	8 cut(s)	803, 1179, 1421, 1727, 1769, 1809, 1962, 2058
TscAI	CASTG	3 cut(s)	663, 2119, 2244
TseFI	GTSAC	1 cut(s)	73
TseI	GCWGC	5 cut(s)	130, 1170, 1990, 2017, 2171
Tsp45I	GTSAC	1 cut(s)	73
TspRI	CASTG	3 cut(s)	663, 2119, 2244
VpaK11BI	GGWCC	3 cut(s)	21, 1523, 2303
XapI	RAATTY	6 cut(s)	86, 1194, 1209, 1243, 1636, 2054
XbaI	TCTAGA	2 cut(s)	886, 1643
XceI	RCATGY	2 cut(s)	1158, 1682
XhoI	CTCGAG	1 cut(s)	1059
XmnI	GAANNNNTTC	2 cut(s)	300, 1210
XspI	CTAG	4 cut(s)	887, 1512, 1601, 1644
ZrmI	AGTACT	1 cut(s)	2353
Zsp2I	ATGCAT	2 cut(s)	868, 1338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.