MD12G1174800.v1.1

Nudix hydrolase 15, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008221
Physical Location & Seq
Reverse (-)
25499391 .. 25500520
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1174800.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGCTAGCATAGAGGATTGGGGAAGAAGCCAAACTCTACACAGGCTTGCCAAGCAGCTTGAGTTTTACAAACCTCCCTCCGCACCAGATAAATCAGAGGATGATAACGAAAGCGATACATCTGTGAACTCTGGTATAAGCTTCAACTCCTTAGCAGTCGGCAATGAAGAGTGTTCTTGTGTCAAGTTGAGATTGAGAGAGAGAAGAGCAGCTGTCCTGATATGCATCTTTGAGGGAGCTGAGGGTGAGCTAAGAGTTATTCTTACTAGGAGATCCATGAATTTGGCATCACACCCAGGAGATGTAGCATTGCCCGGCGGAAAAATGGAGAAGGAAGATGCAGATGAATCTGCAACAGCACTAAGGGAAGCCATGGAAGAGATTGGACTCGATCCAAGCCTAGTTCAAGTTGTTGCAAAGCTAGAGTGCTTTATGTCCCAGCATTTGGTCACCGTTGTCCCAGTGATAGGACTGGTAGCTAGAATTGAAGATTTCAAGCCTGTACTCAACGCCGACGAAGTTGATGCCATTTTTGATGTTCCATTGGAGATGTTTCTCAAGAATGATAATCACAGATTTGAGGACAGAGAATGGATGGGTTGCAAGTACATTCTCCATCTTTTCGATTTTGAATCAGAGCAAGGGCCGTTTCTAATTTGGGGACTAACTGCAAGCATTTTAATTCGGGTGGCCGCTGTCATCTACCAGCAGCCTCCATTATTCCAAGGACACAGCCTCCCTGACTTCCAAGAATTACAAAAAGCCTTGCATAGTGTGCATAATGTTGATGTAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
NUDIX

Protein Analysis

266

Amino Acids

29.68

Weight (kDa)

4.8

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 68 - 198 2.9e-14 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 445
AciI CCGC 3 cut(s) 81, 318, 693
AclWI GGATC 2 cut(s) 267, 386
AcoI YGGCCR 1 cut(s) 690
AcsI RAATTY 1 cut(s) 280
AfaI GTAC 2 cut(s) 504, 608
AfiI CCNNNNNNNGG 2 cut(s) 445, 467
AgsI TTSAA 5 cut(s) 145, 407, 488, 496, 632
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 7 cut(s) 58, 141, 212, 239, 250, 421, 479
AluI AGCT 7 cut(s) 58, 141, 212, 239, 250, 421, 479
AlwI GGATC 2 cut(s) 267, 386
AoxI GGCC 2 cut(s) 644, 690
ApeKI GCWGC 3 cut(s) 55, 209, 709
ApoI RAATTY 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 644
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 2 cut(s) 257, 442
AsuNHI GCTAGC 1 cut(s) 5
BaeI ACNNNNGTAYC 2 cut(s) 108, 141
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 3 cut(s) 67, 221, 721
BccI CCATC 2 cut(s) 589, 624
BceAI ACGGC 1 cut(s) 631
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 1 cut(s) 297
BcnI CCSGG 1 cut(s) 315
BfaI CTAG 5 cut(s) 6, 267, 401, 422, 480
BisI GCNGC 4 cut(s) 56, 210, 693, 710
BlsI GCNGC 4 cut(s) 57, 211, 694, 711
Bme1390I CCNGG 2 cut(s) 297, 315
BmgT120I GGNCC 1 cut(s) 644
BmrFI CCNGG 2 cut(s) 297, 315
BmrI ACTGGG 1 cut(s) 455
BmsI GCATC 4 cut(s) 234, 296, 328, 514
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 455
Bpu10I CCTNAGC 2 cut(s) 151, 240
BpuEI CTTGAG 2 cut(s) 80, 542
BpuMI CCSGG 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 295, 372, 724
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 467
Bse1I ACTGG 2 cut(s) 461, 477
Bse3DI GCAATG 2 cut(s) 169, 308
Bse8I GATNNNNATC 1 cut(s) 224
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 3 cut(s) 295, 372, 724
BseGI GGATG 2 cut(s) 106, 600
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 445, 467
BseMI GCAATG 2 cut(s) 169, 308
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 461, 477
BseXI GCAGC 3 cut(s) 67, 221, 721
BseYI CCCAGC 1 cut(s) 438
BshFI GGCC 2 cut(s) 646, 692
BsiSI CCGG 1 cut(s) 315
BslFI GGGAC 3 cut(s) 421, 443, 675
BslI CCNNNNNNNGG 2 cut(s) 445, 467
BsmFI GGGAC 3 cut(s) 421, 443, 675
BsnI GGCC 2 cut(s) 646, 692
Bsp143I GATC 2 cut(s) 272, 391
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 3 cut(s) 81, 318, 693
BspANI GGCC 2 cut(s) 646, 692
BspCNI CTCAG 1 cut(s) 232
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 267, 386
BspQI GCTCTTC 1 cut(s) 199
BsrDI GCAATG 2 cut(s) 169, 308
BsrI ACTGG 2 cut(s) 461, 477
BssECI CCNNGG 3 cut(s) 295, 372, 724
BssMI GATC 2 cut(s) 272, 391
BssT1I CCWWGG 2 cut(s) 372, 724
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 3 cut(s) 162, 199, 372
BstAPI GCANNNNNTGC 1 cut(s) 775
BstC8I GCNNGC 3 cut(s) 7, 48, 673
BstDEI CTNAG 4 cut(s) 151, 240, 251, 362
BstDSI CCRYGG 1 cut(s) 372
BstEII GGTNACC 1 cut(s) 448
BstF5I GGATG 2 cut(s) 106, 600
BstKTI GATC 2 cut(s) 275, 394
BstMBI GATC 2 cut(s) 272, 391
BstMWI GCNNNNNNNGC 2 cut(s) 52, 775
BstNI CCWGG 1 cut(s) 297
BstPI GGTNACC 1 cut(s) 448
BstSCI CCNGG 2 cut(s) 295, 313
BstV1I GCAGC 3 cut(s) 67, 221, 721
BstX2I RGATCY 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 1 cut(s) 272
BsuRI GGCC 2 cut(s) 646, 692
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 2 cut(s) 106, 600
BtsIMutI CAGTG 1 cut(s) 468
Cac8I GCNNGC 3 cut(s) 7, 48, 673
Cfr13I GGNCC 1 cut(s) 644
Csp6I GTAC 2 cut(s) 503, 607
CviAII CATG 3 cut(s) 277, 373, 795
CviQI GTAC 2 cut(s) 503, 607
DdeI CTNAG 4 cut(s) 151, 240, 251, 362
DpnI GATC 2 cut(s) 274, 393
DpnII GATC 2 cut(s) 272, 391
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 3 cut(s) 162, 199, 372
EarI CTCTTC 3 cut(s) 162, 199, 372
EciI GGCGGA 1 cut(s) 333
Eco130I CCWWGG 2 cut(s) 372, 724
Eco91I GGTNACC 1 cut(s) 448
EcoO65I GGTNACC 1 cut(s) 448
EcoRII CCWGG 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 372, 724
EcoT22I ATGCAT 1 cut(s) 227
ErhI CCWWGG 2 cut(s) 372, 724
FaeI CATG 3 cut(s) 280, 376, 798
FaiI YATR 9 cut(s) 11, 137, 223, 278, 374, 434, 771, 780, 796
FaqI GGGAC 3 cut(s) 421, 443, 675
FatI CATG 3 cut(s) 276, 372, 794
Fnu4HI GCNGC 4 cut(s) 56, 210, 693, 710
FokI GGATG 2 cut(s) 113, 607
Fsp4HI GCNGC 4 cut(s) 56, 210, 693, 710
FspBI CTAG 5 cut(s) 6, 267, 401, 422, 480
GluI GCNGC 4 cut(s) 56, 210, 693, 710
GsaI CCCAGC 1 cut(s) 442
HaeIII GGCC 2 cut(s) 646, 692
HapII CCGG 1 cut(s) 315
Hin1II CATG 3 cut(s) 280, 376, 798
HindIII AAGCTT 1 cut(s) 139
HinfI GANTC 3 cut(s) 347, 387, 632
HpaII CCGG 1 cut(s) 315
HphI GGTGA 2 cut(s) 257, 442
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 2 cut(s) 97, 637
Hpy188III TCNNGA 2 cut(s) 217, 559
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 518
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 8 cut(s) 225, 341, 353, 416, 603, 671, 769, 778
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 775
HpyF3I CTNAG 4 cut(s) 151, 240, 251, 362
Hsp92II CATG 3 cut(s) 280, 376, 798
Kzo9I GATC 2 cut(s) 272, 391
LguI GCTCTTC 1 cut(s) 199
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 3 cut(s) 67, 221, 721
LweI GCATC 4 cut(s) 234, 296, 328, 514
MaeI CTAG 5 cut(s) 6, 267, 401, 422, 480
MaeIII GTNAC 2 cut(s) 448, 790
MalI GATC 2 cut(s) 274, 393
MboI GATC 2 cut(s) 272, 391
MboII GAAGA 6 cut(s) 36, 179, 216, 347, 389, 500
MflI RGATCY 1 cut(s) 272
MluCI AATT 5 cut(s) 280, 483, 654, 681, 752
MlyI GAGTC 1 cut(s) 381
MnlI CCTC 9 cut(s) 7, 84, 88, 91, 226, 235, 574, 723, 746
Mph1103I ATGCAT 1 cut(s) 227
MseI TTAA 1 cut(s) 680
MspA1I CMGCKG 2 cut(s) 212, 695
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 2 cut(s) 297, 315
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 52, 775
NciI CCSGG 1 cut(s) 315
NcoI CCATGG 1 cut(s) 372
NdeII GATC 2 cut(s) 272, 391
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 3 cut(s) 280, 376, 798
NmuCI GTSAC 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 227
PciSI GCTCTTC 1 cut(s) 199
PfeI GAWTC 2 cut(s) 347, 632
PflMI CCANNNNNTGG 1 cut(s) 445
PkrI GCNGC 4 cut(s) 57, 211, 694, 711
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 295
PspEI GGTNACC 1 cut(s) 448
PspFI CCCAGC 1 cut(s) 438
PspGI CCWGG 1 cut(s) 295
PspPI GGNCC 1 cut(s) 644
PsuI RGATCY 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 2 cut(s) 504, 608
RsaNI GTAC 2 cut(s) 503, 607
SapI GCTCTTC 1 cut(s) 199
SaqAI TTAA 1 cut(s) 680
SatI GCNGC 4 cut(s) 56, 210, 693, 710
Sau3AI GATC 2 cut(s) 272, 391
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 1 cut(s) 381
ScrFI CCNGG 2 cut(s) 297, 315
SetI ASST 8 cut(s) 60, 76, 143, 214, 241, 252, 423, 481
SfaNI GCATC 4 cut(s) 234, 296, 328, 514
SmlI CTYRAG 2 cut(s) 59, 557
SmoI CTYRAG 2 cut(s) 59, 557
Sse9I AATT 5 cut(s) 280, 483, 654, 681, 752
SsiI CCGC 3 cut(s) 81, 318, 693
SspMI CTAG 5 cut(s) 6, 267, 401, 422, 480
StyD4I CCNGG 2 cut(s) 295, 313
StyI CCWWGG 2 cut(s) 372, 724
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 2 cut(s) 390, 624
TasI AATT 5 cut(s) 280, 483, 654, 681, 752
TatI WGTACW 2 cut(s) 502, 606
TauI GCSGC 1 cut(s) 695
TfiI GAWTC 2 cut(s) 347, 632
Tru1I TTAA 1 cut(s) 680
Tru9I TTAA 1 cut(s) 680
TscAI CASTG 1 cut(s) 468
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 3 cut(s) 55, 209, 709
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 3 cut(s) 180, 293, 360
TspRI CASTG 1 cut(s) 468
Van91I CCANNNNNTGG 1 cut(s) 445
XapI RAATTY 1 cut(s) 280
XspI CTAG 5 cut(s) 6, 267, 401, 422, 480
Zsp2I ATGCAT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.