Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011993

Physical Location & Seq

Genomic Coordinates Forward (+)

25947655 .. 25952028

Gene Structure 4374 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1179000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1293 bp

ATGAATCAAATGGCCAAAAAGCAGAAAATTGTGCTTGTTCCCACAAAAACATGGAATATCCGAAGCAGCTTGCATTCCCTCATTGCAATCCTTGTGACTACTGTACTAGTTGCAGCTGTTTATCTAACCCTAGATGGTGGACTGCAATTGTTGTATCAAGATTATTGTCATATAACAACCACACTGGAAACTTCAGCAGCAGCCGCAGCTGGGGAAAACCCTAAATTGCCAAGCAGCAGCAGATGCAATTTGTTTTCAGGGAAATGGGTTTTCGACAACCAGTCTTATCCTCTGTACAAGGAGAAACAGTGCACTTATATGTCAGACCAATTGGCTTGTGAGAAGTTTGGGAGAAAAGACTTGAGCTATCAGAACTGGAGGTGGCAGCCTCACCAATGCGACCTCCCTAGGTTCAACGCGACAGCGTTGCTTGAGAGGCTGAGGAATAAGAGGCTTGTGTTTGTTGGGGACTCGTTGAATAGAGGTCAATGGGTTTCTATGGTATGCCTGGTTGACTCAGCCATCAGTCCAAAGCTCAAATCCATGCAGACAAAATTCAATGGCTCCTTGCACATCTTCAAGGCCACTGAATACAATGCCACAGTTGAGTTCTACTGGGCCCCATTGTTGGTGGAGTCAAACTCCGATGACCCAGTGAACCACCGATTGCCAGATCGGATTGTAAGAGTGCAGGCAATTGAAAAACATGCAAGGCACTGGACGGACGCCGACTTTCTTGTTTTCAATTCATATCTTTGGTGGAGAAGGGATCAAATGAATGTCTTGTGGGGATCTTCTGAGAGCTCAGACGCGATATACAAAGATGTCGAGATGTTTAGGGTTTATGAGATGGCTCTACAGACGTGGTCGGATTGGTTGGAGGTCCATGTCAATCGAACCAAGACGAAATTGTTTTTTGTTAGCATGTCACCCACTCACCAAAGTGCTAAAGAATGGGGTGGGGAAAGTGGTGAGAATTGCTACAAAGAAACAAAGCCAATCACTGAGGAGGGATATTGGGGAAATGGATCAGATCCCAAAATGCTACGTTTAGTTGAGAATGCCCTAGATGGACTGAAAGTAAGAGGTTTGGATGTTCAAATGCTCAACATAACACAACTCTCAGAATACAGGAAAGAAGGGCATCCATCAATTTACAGGAAGCAATGGGAGCCTCTGACTGAAGAACAAGCATCAAACCCTAGCACTTTTGCAGATTGCATACACTGGTGCCTCCCTGGAGTGCCTGACGTGTGGAATGAGATTCTCTATGCTTACATTTTCCAACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0070589 GO:0070592 GO:0071554 GO:0071704 GO:0071840 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

431

Amino Acids

49.77

Weight (kDa)

6.83

Isoelectric Point (pI)

46.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	81 - 134	2.4e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	135 - 425	3.9e-93	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011993)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38320 AT2G38320
fragaria_vesca	FvH4_6g10730 FvH4_6g10730 FvH4_6g10730
malus_domestica	MD12G1179000.v1.1
prunus_persica	Prupe.6G284100_v2.0.a1
pyrus_communis	pycom12g16890
rosa_chinensis	RchiOBHm_Chr3g0461761
rosa_laevigata	RLG00000024902
rosa_multiflora	Rmu_sc0004308.1_g000002
rosa_roxburghii	Rroxscaffold_6G00417980
rosa_rugosa	Rorug03G0053300 Rorug03G0053400
rosa_samantha	Rh3AG111500 Rh3BG114500 Rh3CG116800 Rh3DG116500
rosa_wichuraiana	Rw3G009440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1230
AccII	CGCG	2 cut(s)	419, 812
AciI	CCGC	1 cut(s)	204
AclWI	GGATC	4 cut(s)	777, 799, 1028, 1036
AcoI	YGGCCR	1 cut(s)	12
AcsI	RAATTY	1 cut(s)	554
AcuI	CTGAAG	2 cut(s)	177, 1203
AcyI	GRCGYC	1 cut(s)	726
AfaI	GTAC	2 cut(s)	105, 296
AfiI	CCNNNNNNNGG	2 cut(s)	210, 628
AflIII	ACRYGT	1 cut(s)	1251
AgsI	TTSAA	7 cut(s)	415, 478, 559, 580, 701, 745, 1100
AhdI	GACNNNNNGTC	1 cut(s)	280
AhlI	ACTAGT	1 cut(s)	106
AjiI	CACGTC	2 cut(s)	864, 1252
AjnI	CCWGG	2 cut(s)	507, 1237
AleI	CACNNNNGTG	1 cut(s)	942
AluBI	AGCT	6 cut(s)	69, 116, 209, 366, 535, 804
AluI	AGCT	6 cut(s)	69, 116, 209, 366, 535, 804
Alw21I	GWGCWC	2 cut(s)	314, 806
Alw44I	GTGCAC	1 cut(s)	310
AlwI	GGATC	4 cut(s)	777, 799, 1028, 1036
AoxI	GGCC	3 cut(s)	12, 582, 618
ApaI	GGGCCC	1 cut(s)	622
ApaLI	GTGCAC	1 cut(s)	310
ApeKI	GCWGC	8 cut(s)	66, 113, 197, 200, 206, 234, 237, 385
ApoI	RAATTY	1 cut(s)	554
AspA2I	CCTAGG	1 cut(s)	407
AspS9I	GGNCC	3 cut(s)	618, 619, 883
AsuHPI	GGTGA	4 cut(s)	383, 921, 929, 983
AvaII	GGWCC	1 cut(s)	883
AvrII	CCTAGG	1 cut(s)	407
BaeGI	GKGCMC	2 cut(s)	314, 622
BalI	TGGCCA	1 cut(s)	14
BanI	GGYRCC	1 cut(s)	1230
BanII	GRGCYC	2 cut(s)	622, 806
Bbv12I	GWGCWC	2 cut(s)	314, 806
BbvCI	CCTCAGC	1 cut(s)	440
BbvI	GCAGC	8 cut(s)	78, 125, 209, 212, 218, 246, 249, 397
BccI	CCATC	5 cut(s)	128, 530, 844, 1064, 1156
BcgI	CGANNNNNNTGC	2 cut(s)	409, 443
BciT130I	CCWGG	2 cut(s)	509, 1239
BcuI	ACTAGT	1 cut(s)	106
BfaI	CTAG	5 cut(s)	107, 131, 408, 1067, 1203
BfmI	CTRYAG	1 cut(s)	857
BisI	GCNGC	9 cut(s)	67, 114, 198, 201, 204, 207, 235, 238, 386
BlnI	CCTAGG	1 cut(s)	407
BlsI	GCNGC	9 cut(s)	68, 115, 199, 202, 205, 208, 236, 239, 387
Bme1390I	CCNGG	2 cut(s)	509, 1239
Bme18I	GGWCC	1 cut(s)	883
BmeRI	GACNNNNNGTC	1 cut(s)	280
BmgBI	CACGTC	2 cut(s)	864, 1252
BmgT120I	GGNCC	3 cut(s)	618, 619, 883
BmiI	GGNNCC	5 cut(s)	565, 620, 621, 1173, 1232
BmrFI	CCNGG	2 cut(s)	509, 1239
BmrI	ACTGGG	2 cut(s)	625, 647
BmsI	GCATC	3 cut(s)	233, 1153, 1202
BmuI	ACTGGG	2 cut(s)	625, 647
BplI	GAGNNNNNCTC	2 cut(s)	626, 658
BpmI	CTGGAG	2 cut(s)	397, 1260
Bpu10I	CCTNAGC	1 cut(s)	440
BpuEI	CTTGAG	2 cut(s)	382, 452
BsaHI	GRCGYC	1 cut(s)	726
BsaJI	CCNNGG	2 cut(s)	407, 1237
BsaXI	ACNNNNNCTCC	2 cut(s)	293, 323
Bsc4I	CCNNNNNNNGG	2 cut(s)	210, 628
Bse1I	ACTGG	7 cut(s)	189, 280, 380, 620, 653, 722, 1232
Bse3DI	GCAATG	2 cut(s)	81, 1172
BseBI	CCWGG	2 cut(s)	509, 1239
BseDI	CCNNGG	2 cut(s)	407, 1237
BseGI	GGATG	2 cut(s)	1099, 1144
BseLI	CCNNNNNNNGG	2 cut(s)	210, 628
BseMI	GCAATG	2 cut(s)	81, 1172
BseMII	CTCAG	6 cut(s)	431, 531, 789, 819, 996, 1137
BseNI	ACTGG	7 cut(s)	189, 280, 380, 620, 653, 722, 1232
BseRI	GAGGAG	1 cut(s)	1022
BseSI	GKGCMC	2 cut(s)	314, 622
BseXI	GCAGC	8 cut(s)	78, 125, 209, 212, 218, 246, 249, 397
BseYI	CCCAGC	1 cut(s)	209
BsgI	GTGCAG	1 cut(s)	710
Bsh1236I	CGCG	2 cut(s)	419, 812
BshFI	GGCC	3 cut(s)	14, 584, 620
BshNI	GGYRCC	1 cut(s)	1230
BsiHKAI	GWGCWC	2 cut(s)	314, 806
BslFI	GGGAC	1 cut(s)	482
BslI	CCNNNNNNNGG	2 cut(s)	210, 628
BsmFI	GGGAC	1 cut(s)	482
BsmI	GAATGC	2 cut(s)	73, 1066
BsnI	GGCC	3 cut(s)	14, 584, 620
Bsp120I	GGGCCC	1 cut(s)	618
Bsp1286I	GDGCHC	3 cut(s)	314, 622, 806
Bsp1407I	TGTACA	1 cut(s)	294
Bsp143I	GATC	5 cut(s)	673, 769, 791, 1028, 1033
BspACI	CCGC	1 cut(s)	204
BspANI	GGCC	3 cut(s)	14, 584, 620
BspCNI	CTCAG	6 cut(s)	432, 530, 790, 818, 997, 1136
BspFNI	CGCG	2 cut(s)	419, 812
BspLI	GGNNCC	5 cut(s)	565, 620, 621, 1173, 1232
BspPI	GGATC	4 cut(s)	777, 799, 1028, 1036
BspT107I	GGYRCC	1 cut(s)	1230
BsrDI	GCAATG	2 cut(s)	81, 1172
BsrGI	TGTACA	1 cut(s)	294
BsrI	ACTGG	7 cut(s)	189, 280, 380, 620, 653, 722, 1232
BssECI	CCNNGG	2 cut(s)	407, 1237
BssMI	GATC	5 cut(s)	673, 769, 791, 1028, 1033
BssNI	GRCGYC	1 cut(s)	726
BssT1I	CCWWGG	1 cut(s)	407
Bst2UI	CCWGG	2 cut(s)	509, 1239
Bst4CI	ACNGT	3 cut(s)	103, 309, 604
BstACI	GRCGYC	1 cut(s)	726
BstAPI	GCANNNNNTGC	1 cut(s)	243
BstAUI	TGTACA	1 cut(s)	294
BstC8I	GCNNGC	2 cut(s)	71, 693
BstDEI	CTNAG	6 cut(s)	440, 517, 798, 805, 1005, 1123
BstF5I	GGATG	2 cut(s)	1099, 1144
BstFNI	CGCG	2 cut(s)	419, 812
BstKTI	GATC	5 cut(s)	676, 772, 794, 1031, 1036
BstMBI	GATC	5 cut(s)	673, 769, 791, 1028, 1033
BstMWI	GCNNNNNNNGC	5 cut(s)	203, 206, 243, 436, 1171
BstNI	CCWGG	2 cut(s)	509, 1239
BstNSI	RCATGY	2 cut(s)	710, 928
BstSCI	CCNGG	2 cut(s)	507, 1237
BstSFI	CTRYAG	1 cut(s)	857
BstSLI	GKGCMC	2 cut(s)	314, 622
BstUI	CGCG	2 cut(s)	419, 812
BstV1I	GCAGC	8 cut(s)	78, 125, 209, 212, 218, 246, 249, 397
BstX2I	RGATCY	2 cut(s)	791, 1033
BstYI	RGATCY	2 cut(s)	791, 1033
BsuRI	GGCC	3 cut(s)	14, 584, 620
BtrI	CACGTC	2 cut(s)	864, 1252
BtsCI	GGATG	2 cut(s)	1099, 1144
BtsIMutI	CAGTG	7 cut(s)	182, 314, 585, 660, 715, 1002, 1225
Cac8I	GCNNGC	2 cut(s)	71, 693
Cfr13I	GGNCC	3 cut(s)	618, 619, 883
CseI	GACGC	2 cut(s)	734, 818
Csp6I	GTAC	2 cut(s)	104, 295
CviAII	CATG	5 cut(s)	51, 544, 707, 887, 925
CviQI	GTAC	2 cut(s)	104, 295
DdeI	CTNAG	6 cut(s)	440, 517, 798, 805, 1005, 1123
DpnI	GATC	5 cut(s)	675, 771, 793, 1030, 1035
DpnII	GATC	5 cut(s)	673, 769, 791, 1028, 1033
DriI	GACNNNNNGTC	1 cut(s)	280
EaeI	YGGCCR	1 cut(s)	12
Eam1105I	GACNNNNNGTC	1 cut(s)	280
Ecl136II	GAGCTC	1 cut(s)	804
Eco130I	CCWWGG	1 cut(s)	407
Eco24I	GRGCYC	2 cut(s)	622, 806
Eco47I	GGWCC	1 cut(s)	883
Eco53kI	GAGCTC	1 cut(s)	804
Eco57I	CTGAAG	2 cut(s)	177, 1203
EcoICRI	GAGCTC	1 cut(s)	804
EcoO109I	RGGNCCY	1 cut(s)	619
EcoRII	CCWGG	2 cut(s)	507, 1237
EcoT14I	CCWWGG	1 cut(s)	407
EcoT38I	GRGCYC	2 cut(s)	622, 806
ErhI	CCWWGG	1 cut(s)	407
FaeI	CATG	5 cut(s)	54, 547, 710, 890, 928
FaqI	GGGAC	1 cut(s)	482
FatI	CATG	5 cut(s)	50, 543, 706, 886, 924
Fnu4HI	GCNGC	9 cut(s)	67, 114, 198, 201, 204, 207, 235, 238, 386
FokI	GGATG	2 cut(s)	1106, 1131
FriOI	GRGCYC	2 cut(s)	622, 806
Fsp4HI	GCNGC	9 cut(s)	67, 114, 198, 201, 204, 207, 235, 238, 386
FspBI	CTAG	5 cut(s)	107, 131, 408, 1067, 1203
GluI	GCNGC	9 cut(s)	67, 114, 198, 201, 204, 207, 235, 238, 386
GsaI	CCCAGC	1 cut(s)	213
GsuI	CTGGAG	2 cut(s)	397, 1260
HaeIII	GGCC	3 cut(s)	14, 584, 620
HgaI	GACGC	2 cut(s)	734, 818
Hin1I	GRCGYC	1 cut(s)	726
Hin1II	CATG	5 cut(s)	54, 547, 710, 890, 928
HincII	GTYRAC	1 cut(s)	514
HindII	GTYRAC	1 cut(s)	514
HinfI	GANTC	5 cut(s)	4, 470, 515, 635, 1264
HphI	GGTGA	4 cut(s)	383, 921, 929, 983
Hpy166II	GTNNAC	4 cut(s)	140, 312, 514, 658
Hpy188III	TCNNGA	2 cut(s)	158, 829
Hpy8I	GTNNAC	4 cut(s)	140, 312, 514, 658
HpyAV	CCTTC	2 cut(s)	759, 1133
HpyCH4III	ACNGT	3 cut(s)	103, 309, 604
HpyCH4IV	ACGT	3 cut(s)	863, 1048, 1251
HpyF10VI	GCNNNNNNNGC	5 cut(s)	203, 206, 243, 436, 1171
HpyF3I	CTNAG	6 cut(s)	440, 517, 798, 805, 1005, 1123
HpySE526I	ACGT	3 cut(s)	863, 1048, 1251
Hsp92I	GRCGYC	1 cut(s)	726
Hsp92II	CATG	5 cut(s)	54, 547, 710, 890, 928
Kzo9I	GATC	5 cut(s)	673, 769, 791, 1028, 1033
LmnI	GCTCC	2 cut(s)	569, 1171
Lsp1109I	GCAGC	8 cut(s)	78, 125, 209, 212, 218, 246, 249, 397
LweI	GCATC	3 cut(s)	233, 1153, 1202
MaeI	CTAG	5 cut(s)	107, 131, 408, 1067, 1203
MaeII	ACGT	3 cut(s)	863, 1048, 1251
MaeIII	GTNAC	2 cut(s)	94, 927
MalI	GATC	5 cut(s)	675, 771, 793, 1030, 1035
MboI	GATC	5 cut(s)	673, 769, 791, 1028, 1033
MboII	GAAGA	3 cut(s)	568, 786, 1196
MfeI	CAATTG	3 cut(s)	146, 329, 696
MflI	RGATCY	2 cut(s)	791, 1033
MhlI	GDGCHC	3 cut(s)	314, 622, 806
MlsI	TGGCCA	1 cut(s)	14
MluNI	TGGCCA	1 cut(s)	14
MlyI	GAGTC	3 cut(s)	464, 509, 644
MmeI	TCCRAC	2 cut(s)	849, 858
Mox20I	TGGCCA	1 cut(s)	14
MscI	TGGCCA	1 cut(s)	14
MslI	CAYNNNNRTG	2 cut(s)	317, 942
Msp20I	TGGCCA	1 cut(s)	14
MspA1I	CMGCKG	2 cut(s)	116, 209
MspR9I	CCNGG	2 cut(s)	509, 1239
MunI	CAATTG	3 cut(s)	146, 329, 696
Mva1269I	GAATGC	2 cut(s)	73, 1066
MvaI	CCWGG	2 cut(s)	509, 1239
MvnI	CGCG	2 cut(s)	419, 812
MwoI	GCNNNNNNNGC	5 cut(s)	203, 206, 243, 436, 1171
NdeII	GATC	5 cut(s)	673, 769, 791, 1028, 1033
NlaIII	CATG	5 cut(s)	54, 547, 710, 890, 928
NlaIV	GGNNCC	5 cut(s)	565, 620, 621, 1173, 1232
NmuCI	GTSAC	2 cut(s)	94, 927
NspI	RCATGY	2 cut(s)	710, 928
OliI	CACNNNNGTG	1 cut(s)	942
PctI	GAATGC	2 cut(s)	73, 1066
PfeI	GAWTC	2 cut(s)	4, 1264
PflFI	GACNNNGTC	1 cut(s)	865
PkrI	GCNGC	9 cut(s)	68, 115, 199, 202, 205, 208, 236, 239, 387
PleI	GAGTC	3 cut(s)	464, 509, 643
PpsI	GAGTC	3 cut(s)	464, 509, 643
Psp124BI	GAGCTC	1 cut(s)	806
Psp6I	CCWGG	2 cut(s)	507, 1237
PspFI	CCCAGC	1 cut(s)	209
PspGI	CCWGG	2 cut(s)	507, 1237
PspN4I	GGNNCC	5 cut(s)	565, 620, 621, 1173, 1232
PspOMI	GGGCCC	1 cut(s)	618
PspPI	GGNCC	3 cut(s)	618, 619, 883
PsuI	RGATCY	2 cut(s)	791, 1033
PsyI	GACNNNGTC	1 cut(s)	865
PvuII	CAGCTG	2 cut(s)	116, 209
RsaI	GTAC	2 cut(s)	105, 296
RsaNI	GTAC	2 cut(s)	104, 295
RseI	CAYNNNNRTG	2 cut(s)	317, 942
SacI	GAGCTC	1 cut(s)	806
SatI	GCNGC	9 cut(s)	67, 114, 198, 201, 204, 207, 235, 238, 386
Sau3AI	GATC	5 cut(s)	673, 769, 791, 1028, 1033
Sau96I	GGNCC	3 cut(s)	618, 619, 883
SchI	GAGTC	3 cut(s)	464, 509, 644
ScrFI	CCNGG	2 cut(s)	509, 1239
SduI	GDGCHC	3 cut(s)	314, 622, 806
SfaNI	GCATC	3 cut(s)	233, 1153, 1202
SfcI	CTRYAG	1 cut(s)	857
SinI	GGWCC	1 cut(s)	883
SmiMI	CAYNNNNRTG	2 cut(s)	317, 942
SmlI	CTYRAG	2 cut(s)	361, 431
SmoI	CTYRAG	2 cut(s)	361, 431
SpeI	ACTAGT	1 cut(s)	106
SsiI	CCGC	1 cut(s)	204
SspMI	CTAG	5 cut(s)	107, 131, 408, 1067, 1203
SstI	GAGCTC	1 cut(s)	806
StyD4I	CCNGG	2 cut(s)	507, 1237
StyI	CCWWGG	1 cut(s)	407
TaaI	ACNGT	3 cut(s)	103, 309, 604
TaiI	ACGT	3 cut(s)	866, 1051, 1254
TaqI	TCGA	3 cut(s)	273, 828, 895
TatI	WGTACW	2 cut(s)	103, 294
TauI	GCSGC	1 cut(s)	206
TfiI	GAWTC	2 cut(s)	4, 1264
TscAI	CASTG	7 cut(s)	189, 314, 592, 660, 722, 1009, 1232
TseFI	GTSAC	2 cut(s)	94, 927
TseI	GCWGC	8 cut(s)	66, 113, 197, 200, 206, 234, 237, 385
Tsp45I	GTSAC	2 cut(s)	94, 927
TspDTI	ATGAA	3 cut(s)	17, 738, 791
TspGWI	ACGGA	1 cut(s)	737
TspRI	CASTG	7 cut(s)	189, 314, 592, 660, 722, 1009, 1232
Tth111I	GACNNNGTC	1 cut(s)	865
VneI	GTGCAC	1 cut(s)	310
VpaK11BI	GGWCC	1 cut(s)	883
XapI	RAATTY	1 cut(s)	554
XceI	RCATGY	2 cut(s)	710, 928
XmaJI	CCTAGG	1 cut(s)	407
XspI	CTAG	5 cut(s)	107, 131, 408, 1067, 1203

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.