Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0011993

Physical Location & Seq

Genomic Coordinates Reverse (-)

9739342 .. 9742158

Gene Structure 2817 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ42815

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGCCTAAGCATAAGCATCAAATAGTTCCTAATCCCAAACCTTGGGGTTCAATCAGAAGCAGCTTGCATTCCCTCATTGCAGTTCTTGTGGCAACTCTTCTCGTTGCAACTGTTTATCTTACCCAAGAATATGGTGTTCAGTTTTTAGAAGATTGTACTGCTTCTACTACAGTGAAAACTTCTGAGGATAGCTGCAATTTGTTTTCAGGGAAATGGGTATTTGATAACCAGTCTTATCCTTTATACAAGGGGTTACAGTGTACTTATATGTCTGACCAATTGGCCTGTGAGAAGTTTGGGAGAAAAGACTTAAGCTTCCAGAACTGGAGATGGCAACCCCATCAATGTGACCTCCCTAGGTCTCTTCCTGTCCCTTGCTCTCTCCTTTTGTTTTTGTTTGAGTTAATGCATTTTCAGTCCCTATTTAGGCTTCGCTCCGTTATGTCATTGTTTTTCATTTCTCTAACAGAATCTCTTTTATTATTTGACAAAATCAAGGTTCGACCTAAAATTTTAGCTAGCAACTTGAGACAAACTCTTAGGTTCAATGCCACAGCGTTGCTTGAGAGGCTAAGGAACAAGAGGCTTGTGTATGTTGGGGATTCCTTGAATCGAAATCAATGGGTTTCCATGGTTTGCCTGATAGAGTCAGCTGTTCCTCCAGAGCTCAAGTCCATGTACACCAATGGCTCCTTGACCACCTTCAAAGCAACTGAATACAATGCCACAATTGAGTTCTATTGGGCTCCATTGCTAGTGGAATCGAACTCAGATCACCCGATGAATCACCGGTTACCGGAACGGATTGTTAGGGTTCAGGCAATTGAAAAGCATGCTAGACATTGGACTGATGCAGACTTTCTTGTTTTCAATTCCTATCTTTGGTGGAGAAGGTCCATGATGAGTGTCTTGTGGGGATCTTTTGAAAGCTCTGATGGAATTTACAGAGAGGTTGGGATGCCTAGAGTGTATGAAATGGCTCTAAAGACTTGGTCGGATTGGTTGGAGGTCCATGTTAATCCAAACAAGACCAAGTTGTTCTTTGTTAGCATGTCACCTACTCACCAAAGCGCTGAAGAATGGGGTGTGACTACTGGCGAGAATTGCTACGGAGAAACTGAACCGATTAAGGAGGAGGGCTACAAGGGAAATGCATCGGATCCAAAAATGATGCGTGTTGTTGAGAATGTACTTGGGGAGCTGAAAACAAGAGGTTTGGATGTGCAAATACTCAATATAACGCAACTCTCAGAGTACAGAAAAGAAGGTCATCCATCAATTTATAGGAAGCAGTGGGATGCTCTAACAGAAAAGCAATTAGCAAAACCTAGCAGTTTTGCAGATTGTATACATTGGTGCCTCCCTGGAGTGCCAGACGTTTGGAATGAGCTCCTCTATGCTTACATTTTTCAAATGACAGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0070589 GO:0070592 GO:0071554 GO:0071704 GO:0071840 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

474

Amino Acids

54.96

Weight (kDa)

7.96

Isoelectric Point (pI)

49.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	64 - 117	1.3e-21	PMR5 N terminal Domain
PC-Esterase	PF13839	181 - 467	7.9e-93	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011993)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38320 AT2G38320
fragaria_vesca	FvH4_6g10730 FvH4_6g10730 FvH4_6g10730
malus_domestica	MD12G1179000.v1.1
prunus_persica	Prupe.6G284100_v2.0.a1
pyrus_communis	pycom12g16890
rosa_chinensis	RchiOBHm_Chr3g0461761
rosa_laevigata	RLG00000024902
rosa_multiflora	Rmu_sc0004308.1_g000002
rosa_roxburghii	Rroxscaffold_6G00417980
rosa_rugosa	Rorug03G0053300 Rorug03G0053400
rosa_samantha	Rh3AG111500 Rh3BG114500 Rh3CG116800 Rh3DG116500
rosa_wichuraiana	Rw3G009440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1356
AccB7I	CCANNNNNTGG	1 cut(s)	42
AccI	GTMKAC	1 cut(s)	1348
AclWI	GGATC	3 cut(s)	925, 1154, 1167
AcsI	RAATTY	2 cut(s)	510, 939
AcuI	CTGAAG	1 cut(s)	1095
AfaI	GTAC	5 cut(s)	157, 262, 680, 1191, 1256
AfeI	AGCGCT	1 cut(s)	1072
AfiI	CCNNNNNNNGG	4 cut(s)	42, 426, 796, 882
AflII	CTTAAG	1 cut(s)	310
AgeI	ACCGGT	1 cut(s)	789
AgsI	TTSAA	8 cut(s)	51, 547, 610, 706, 827, 871, 926, 1412
AjnI	CCWGG	1 cut(s)	1363
AluBI	AGCT	9 cut(s)	63, 192, 315, 518, 653, 667, 930, 1201, 1390
AluI	AGCT	9 cut(s)	63, 192, 315, 518, 653, 667, 930, 1201, 1390
Alw21I	GWGCWC	2 cut(s)	669, 1392
Alw26I	GTCTC	2 cut(s)	366, 523
AlwI	GGATC	3 cut(s)	925, 1154, 1167
Aor51HI	AGCGCT	1 cut(s)	1072
AoxI	GGCC	1 cut(s)	282
ApeKI	GCWGC	2 cut(s)	60, 192
ApoI	RAATTY	2 cut(s)	510, 939
AsiGI	ACCGGT	1 cut(s)	789
AspA2I	CCTAGG	1 cut(s)	356
AspLEI	GCGC	1 cut(s)	1073
AspS9I	GGNCC	2 cut(s)	894, 1009
AsuHPI	GGTGA	4 cut(s)	767, 779, 1047, 1055
AsuNHI	GCTAGC	1 cut(s)	518
AvaII	GGWCC	2 cut(s)	894, 1009
AvrII	CCTAGG	1 cut(s)	356
BamHI	GGATCC	1 cut(s)	1159
BanI	GGYRCC	1 cut(s)	1356
BanII	GRGCYC	3 cut(s)	669, 748, 1392
Bbv12I	GWGCWC	2 cut(s)	669, 1392
BbvI	GCAGC	2 cut(s)	72, 179
BccI	CCATC	4 cut(s)	324, 348, 929, 1282
BciT130I	CCWGG	1 cut(s)	1365
BcoDI	GTCTC	2 cut(s)	366, 523
BfaI	CTAG	6 cut(s)	357, 519, 755, 837, 963, 1329
BfmI	CTRYAG	1 cut(s)	168
BfoI	RGCGCY	1 cut(s)	1074
BfrI	CTTAAG	1 cut(s)	310
BisI	GCNGC	2 cut(s)	61, 193
BlnI	CCTAGG	1 cut(s)	356
BlsI	GCNGC	2 cut(s)	62, 194
Bme1390I	CCNGG	1 cut(s)	1365
Bme18I	GGWCC	2 cut(s)	894, 1009
BmgT120I	GGNCC	2 cut(s)	894, 1009
BmiI	GGNNCC	4 cut(s)	691, 747, 1161, 1358
BmrFI	CCNGG	1 cut(s)	1365
BmsI	GCATC	6 cut(s)	25, 841, 948, 1161, 1163, 1288
BmtI	GCTAGC	1 cut(s)	522
BplI	GAGNNNNNCTC	2 cut(s)	520, 552
BpmI	CTGGAG	3 cut(s)	346, 645, 1386
Bpu10I	CCTNAGC	2 cut(s)	6, 572
BpuEI	CTTGAG	3 cut(s)	547, 584, 653
BsaI	GGTCTC	1 cut(s)	366
BsaJI	CCNNGG	4 cut(s)	41, 356, 630, 1363
BsaWI	WCCGGW	2 cut(s)	789, 796
Bsc4I	CCNNNNNNNGG	4 cut(s)	42, 426, 796, 882
Bse118I	RCCGGY	1 cut(s)	789
Bse1I	ACTGG	3 cut(s)	229, 329, 1099
Bse3DI	GCAATG	2 cut(s)	75, 749
BseBI	CCWGG	1 cut(s)	1365
BseDI	CCNNGG	4 cut(s)	41, 356, 630, 1363
BseGI	GGATG	4 cut(s)	963, 1225, 1270, 1303
BseLI	CCNNNNNNNGG	4 cut(s)	42, 426, 796, 882
BseMI	GCAATG	2 cut(s)	75, 749
BseMII	CTCAG	3 cut(s)	174, 783, 1263
BseNI	ACTGG	3 cut(s)	229, 329, 1099
BseRI	GAGGAG	2 cut(s)	1148, 1382
BseXI	GCAGC	2 cut(s)	72, 179
BshFI	GGCC	1 cut(s)	284
BshNI	GGYRCC	1 cut(s)	1356
BshTI	ACCGGT	1 cut(s)	789
BsiHKAI	GWGCWC	2 cut(s)	669, 1392
BsiSI	CCGG	2 cut(s)	790, 797
BslFI	GGGAC	2 cut(s)	356, 403
BslI	CCNNNNNNNGG	4 cut(s)	42, 426, 796, 882
BsmAI	GTCTC	2 cut(s)	366, 523
BsmFI	GGGAC	2 cut(s)	356, 403
BsmI	GAATGC	1 cut(s)	67
BsnI	GGCC	1 cut(s)	284
Bso31I	GGTCTC	1 cut(s)	366
Bsp1286I	GDGCHC	3 cut(s)	669, 748, 1392
Bsp1407I	TGTACA	1 cut(s)	678
Bsp143I	GATC	3 cut(s)	772, 917, 1159
Bsp19I	CCATGG	1 cut(s)	630
BspANI	GGCC	1 cut(s)	284
BspCNI	CTCAG	3 cut(s)	175, 782, 1262
BspLI	GGNNCC	4 cut(s)	691, 747, 1161, 1358
BspOI	GCTAGC	1 cut(s)	522
BspPI	GGATC	3 cut(s)	925, 1154, 1167
BspT107I	GGYRCC	1 cut(s)	1356
BspTI	CTTAAG	1 cut(s)	310
BspTNI	GGTCTC	1 cut(s)	366
BsrDI	GCAATG	2 cut(s)	75, 749
BsrFI	RCCGGY	1 cut(s)	789
BsrGI	TGTACA	1 cut(s)	678
BsrI	ACTGG	3 cut(s)	229, 329, 1099
BssAI	RCCGGY	1 cut(s)	789
BssECI	CCNNGG	4 cut(s)	41, 356, 630, 1363
BssMI	GATC	3 cut(s)	772, 917, 1159
BssNAI	GTATAC	1 cut(s)	1349
BssT1I	CCWWGG	3 cut(s)	41, 356, 630
Bst1107I	GTATAC	1 cut(s)	1349
Bst2UI	CCWGG	1 cut(s)	1365
Bst4CI	ACNGT	3 cut(s)	112, 172, 258
Bst6I	CTCTTC	2 cut(s)	102, 369
BstAFI	CTTAAG	1 cut(s)	310
BstAUI	TGTACA	1 cut(s)	678
BstC8I	GCNNGC	3 cut(s)	65, 520, 834
BstDEI	CTNAG	6 cut(s)	6, 183, 539, 572, 769, 1249
BstDSI	CCRYGG	1 cut(s)	630
BstEII	GGTNACC	1 cut(s)	792
BstF5I	GGATG	4 cut(s)	963, 1225, 1270, 1303
BstH2I	RGCGCY	1 cut(s)	1074
BstHHI	GCGC	1 cut(s)	1073
BstKTI	GATC	3 cut(s)	775, 920, 1162
BstMAI	GTCTC	2 cut(s)	366, 523
BstMBI	GATC	3 cut(s)	772, 917, 1159
BstMWI	GCNNNNNNNGC	2 cut(s)	568, 1104
BstNI	CCWGG	1 cut(s)	1365
BstNSI	RCATGY	2 cut(s)	836, 1054
BstPI	GGTNACC	1 cut(s)	792
BstSCI	CCNGG	1 cut(s)	1363
BstSFI	CTRYAG	1 cut(s)	168
BstV1I	GCAGC	2 cut(s)	72, 179
BstX2I	RGATCY	2 cut(s)	917, 1159
BstXI	CCANNNNNNTGG	2 cut(s)	131, 1380
BstYI	RGATCY	2 cut(s)	917, 1159
BstZ17I	GTATAC	1 cut(s)	1349
BsuRI	GGCC	1 cut(s)	284
BtgI	CCRYGG	1 cut(s)	630
BtsCI	GGATG	4 cut(s)	963, 1225, 1270, 1303
BtsI	GCAGTG	1 cut(s)	1298
BtsIMutI	CAGTG	3 cut(s)	177, 263, 1298
Cac8I	GCNNGC	3 cut(s)	65, 520, 834
CfoI	GCGC	1 cut(s)	1073
Cfr10I	RCCGGY	1 cut(s)	789
Cfr13I	GGNCC	2 cut(s)	894, 1009
Csp6I	GTAC	5 cut(s)	156, 261, 679, 1190, 1255
CspAI	ACCGGT	1 cut(s)	789
CviAII	CATG	6 cut(s)	631, 676, 833, 898, 1013, 1051
CviQI	GTAC	5 cut(s)	156, 261, 679, 1190, 1255
DdeI	CTNAG	6 cut(s)	6, 183, 539, 572, 769, 1249
DpnI	GATC	3 cut(s)	774, 919, 1161
DpnII	GATC	3 cut(s)	772, 917, 1159
Eam1104I	CTCTTC	2 cut(s)	102, 369
EarI	CTCTTC	2 cut(s)	102, 369
Ecl136II	GAGCTC	2 cut(s)	667, 1390
Eco130I	CCWWGG	3 cut(s)	41, 356, 630
Eco24I	GRGCYC	3 cut(s)	669, 748, 1392
Eco31I	GGTCTC	1 cut(s)	366
Eco47I	GGWCC	2 cut(s)	894, 1009
Eco47III	AGCGCT	1 cut(s)	1072
Eco53kI	GAGCTC	2 cut(s)	667, 1390
Eco57I	CTGAAG	1 cut(s)	1095
Eco91I	GGTNACC	1 cut(s)	792
EcoICRI	GAGCTC	2 cut(s)	667, 1390
EcoO65I	GGTNACC	1 cut(s)	792
EcoRII	CCWGG	1 cut(s)	1363
EcoT14I	CCWWGG	3 cut(s)	41, 356, 630
EcoT22I	ATGCAT	2 cut(s)	411, 1156
EcoT38I	GRGCYC	3 cut(s)	669, 748, 1392
ErhI	CCWWGG	3 cut(s)	41, 356, 630
FaeI	CATG	6 cut(s)	634, 679, 836, 901, 1016, 1054
FalI	AAGNNNNNCTT	2 cut(s)	1025, 1057
FaqI	GGGAC	2 cut(s)	356, 403
FatI	CATG	6 cut(s)	630, 675, 832, 897, 1012, 1050
FblI	GTMKAC	1 cut(s)	1348
Fnu4HI	GCNGC	2 cut(s)	61, 193
FokI	GGATG	4 cut(s)	970, 1232, 1257, 1310
FriOI	GRGCYC	3 cut(s)	669, 748, 1392
Fsp4HI	GCNGC	2 cut(s)	61, 193
FspBI	CTAG	6 cut(s)	357, 519, 755, 837, 963, 1329
GlaI	GCGC	1 cut(s)	1072
GluI	GCNGC	2 cut(s)	61, 193
GsuI	CTGGAG	3 cut(s)	346, 645, 1386
HaeII	RGCGCY	1 cut(s)	1074
HaeIII	GGCC	1 cut(s)	284
HapII	CCGG	2 cut(s)	790, 797
HhaI	GCGC	1 cut(s)	1073
Hin1II	CATG	6 cut(s)	634, 679, 836, 901, 1016, 1054
Hin6I	GCGC	1 cut(s)	1071
HinP1I	GCGC	1 cut(s)	1071
HindIII	AAGCTT	1 cut(s)	313
HinfI	GANTC	6 cut(s)	470, 602, 610, 647, 761, 784
HpaII	CCGG	2 cut(s)	790, 797
HphI	GGTGA	4 cut(s)	767, 779, 1047, 1055
Hpy166II	GTNNAC	3 cut(s)	261, 681, 1349
Hpy188I	TCNGA	8 cut(s)	56, 184, 274, 772, 934, 997, 1159, 1252
Hpy188III	TCNNGA	2 cut(s)	319, 662
Hpy8I	GTNNAC	3 cut(s)	261, 681, 1349
HpyAV	CCTTC	3 cut(s)	712, 885, 1259
HpyCH4III	ACNGT	3 cut(s)	112, 172, 258
HpyCH4IV	ACGT	1 cut(s)	1377
HpyCH4V	TGCA	9 cut(s)	67, 80, 107, 195, 409, 854, 1154, 1225, 1340
HpyF10VI	GCNNNNNNNGC	2 cut(s)	568, 1104
HpyF3I	CTNAG	6 cut(s)	6, 183, 539, 572, 769, 1249
HpySE526I	ACGT	1 cut(s)	1377
Hsp92II	CATG	6 cut(s)	634, 679, 836, 901, 1016, 1054
HspAI	GCGC	1 cut(s)	1071
Kzo9I	GATC	3 cut(s)	772, 917, 1159
LmnI	GCTCC	5 cut(s)	440, 695, 751, 1198, 1395
Lsp1109I	GCAGC	2 cut(s)	72, 179
LweI	GCATC	6 cut(s)	25, 841, 948, 1161, 1163, 1288
MaeI	CTAG	6 cut(s)	357, 519, 755, 837, 963, 1329
MaeII	ACGT	1 cut(s)	1377
MaeIII	GTNAC	5 cut(s)	252, 347, 792, 1053, 1087
MalI	GATC	3 cut(s)	774, 919, 1161
MboI	GATC	3 cut(s)	772, 917, 1159
MboII	GAAGA	4 cut(s)	89, 161, 356, 1088
MfeI	CAATTG	3 cut(s)	278, 729, 822
MflI	RGATCY	2 cut(s)	917, 1159
MhlI	GDGCHC	3 cut(s)	669, 748, 1392
MlyI	GAGTC	1 cut(s)	656
MmeI	TCCRAC	2 cut(s)	975, 984
Mph1103I	ATGCAT	2 cut(s)	411, 1156
MseI	TTAA	4 cut(s)	311, 404, 1017, 1128
MslI	CAYNNNNRTG	1 cut(s)	345
MspA1I	CMGCKG	1 cut(s)	653
MspCI	CTTAAG	1 cut(s)	310
MspI	CCGG	2 cut(s)	790, 797
MspR9I	CCNGG	1 cut(s)	1365
MunI	CAATTG	3 cut(s)	278, 729, 822
Mva1269I	GAATGC	1 cut(s)	67
MvaI	CCWGG	1 cut(s)	1365
MwoI	GCNNNNNNNGC	2 cut(s)	568, 1104
NcoI	CCATGG	1 cut(s)	630
NdeII	GATC	3 cut(s)	772, 917, 1159
NheI	GCTAGC	1 cut(s)	518
NlaIII	CATG	6 cut(s)	634, 679, 836, 901, 1016, 1054
NlaIV	GGNNCC	4 cut(s)	691, 747, 1161, 1358
NmuCI	GTSAC	3 cut(s)	347, 1053, 1087
NsiI	ATGCAT	2 cut(s)	411, 1156
NspI	RCATGY	2 cut(s)	836, 1054
PaeI	GCATGC	1 cut(s)	836
PctI	GAATGC	1 cut(s)	67
PfeI	GAWTC	5 cut(s)	470, 602, 610, 761, 784
PflFI	GACNNNGTC	1 cut(s)	991
PflMI	CCANNNNNTGG	1 cut(s)	42
PinAI	ACCGGT	1 cut(s)	789
PkrI	GCNGC	2 cut(s)	62, 194
PleI	GAGTC	1 cut(s)	655
PpsI	GAGTC	1 cut(s)	655
Psp124BI	GAGCTC	2 cut(s)	669, 1392
Psp6I	CCWGG	1 cut(s)	1363
PspEI	GGTNACC	1 cut(s)	792
PspGI	CCWGG	1 cut(s)	1363
PspN4I	GGNNCC	4 cut(s)	691, 747, 1161, 1358
PspPI	GGNCC	2 cut(s)	894, 1009
PsuI	RGATCY	2 cut(s)	917, 1159
PsyI	GACNNNGTC	1 cut(s)	991
PvuII	CAGCTG	1 cut(s)	653
RsaI	GTAC	5 cut(s)	157, 262, 680, 1191, 1256
RsaNI	GTAC	5 cut(s)	156, 261, 679, 1190, 1255
RseI	CAYNNNNRTG	1 cut(s)	345
SacI	GAGCTC	2 cut(s)	669, 1392
SaqAI	TTAA	4 cut(s)	311, 404, 1017, 1128
SatI	GCNGC	2 cut(s)	61, 193
Sau3AI	GATC	3 cut(s)	772, 917, 1159
Sau96I	GGNCC	2 cut(s)	894, 1009
SchI	GAGTC	1 cut(s)	656
ScrFI	CCNGG	1 cut(s)	1365
SduI	GDGCHC	3 cut(s)	669, 748, 1392
SfaNI	GCATC	6 cut(s)	25, 841, 948, 1161, 1163, 1288
SfcI	CTRYAG	1 cut(s)	168
SinI	GGWCC	2 cut(s)	894, 1009
SmiMI	CAYNNNNRTG	1 cut(s)	345
SmlI	CTYRAG	4 cut(s)	310, 526, 563, 668
SmoI	CTYRAG	4 cut(s)	310, 526, 563, 668
SphI	GCATGC	1 cut(s)	836
SspMI	CTAG	6 cut(s)	357, 519, 755, 837, 963, 1329
SstI	GAGCTC	2 cut(s)	669, 1392
StyD4I	CCNGG	1 cut(s)	1363
StyI	CCWWGG	3 cut(s)	41, 356, 630
TaaI	ACNGT	3 cut(s)	112, 172, 258
TaiI	ACGT	1 cut(s)	1380
TaqI	TCGA	3 cut(s)	502, 613, 764
TatI	WGTACW	5 cut(s)	155, 260, 678, 1189, 1254
TfiI	GAWTC	5 cut(s)	470, 602, 610, 761, 784
Tru1I	TTAA	4 cut(s)	311, 404, 1017, 1128
Tru9I	TTAA	4 cut(s)	311, 404, 1017, 1128
TscAI	CASTG	3 cut(s)	177, 263, 1298
TseFI	GTSAC	3 cut(s)	347, 1053, 1087
TseI	GCWGC	2 cut(s)	60, 192
Tsp45I	GTSAC	3 cut(s)	347, 1053, 1087
TspDTI	ATGAA	3 cut(s)	445, 797, 987
TspGWI	ACGGA	3 cut(s)	427, 817, 1125
TspRI	CASTG	3 cut(s)	177, 263, 1298
Tth111I	GACNNNGTC	1 cut(s)	991
Van91I	CCANNNNNTGG	1 cut(s)	42
Vha464I	CTTAAG	1 cut(s)	310
VpaK11BI	GGWCC	2 cut(s)	894, 1009
XapI	RAATTY	2 cut(s)	510, 939
XceI	RCATGY	2 cut(s)	836, 1054
XmaJI	CCTAGG	1 cut(s)	356
XmiI	GTMKAC	1 cut(s)	1348
XspI	CTAG	6 cut(s)	357, 519, 755, 837, 963, 1329
Zsp2I	ATGCAT	2 cut(s)	411, 1156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.