MD12G1184300.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase SD2-5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013054
Physical Location & Seq
Forward (+)
26450351 .. 26452190
1840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1184300.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGCAGCTATACTTCTTCATCTCCCTCACCATTTGCTTCACTTACGGCTACTCGAAAACCGATGCTCCTCTCGGATACCAATTCAGGTTGCCAGTGCCTTTTGAGAACAGTGTGGGATCCAGCGCTTTTTTGATTGAGAACAATGAAACCAAACCAAGTTTTAAAGTTGGAATGAGTGTGGAACCTGTGAGTGCGAAGTACTCATGCTCCTTGCAAGTCTTTCTTGGAGATGTCAAGGTGTGGTGTTCTGGCCATTACTCAAAGTTTTATACTTCAAAAATATGTGTTCTTGAGCTCTCCCGTGACGGAGATTTGAGATTGAAGGGCCCAAAAGGTAGAGTGGGATGGAGAAGTGGGACTTCTGGACAAGGTGTGCAGATATTGAGGACAGGCAATCTAGTTCTAGTTGATGCACTAGGCAGAATAAAGTGGCAGAGTTTTAATTTTCCAACTGATGTAATGCTTTGGGGACAGAGACTAAGCGTGGCTTCTAGGTTGACTTCATTCCCAAGCAACTCCACTTCATACTATTCTCTCGAAATTGAACCCAGCAGGATTGCTCTCTACTTGAATTCTGGTAAGTGGAACTATTCCTATTGGGAATTCAAGCCCACCGAGAACAGAAACATTGCTTATATTCAATTGGGTCCAAAGGGGTTAGAGTTATTCAGTTATAATCAAAAGAAAATTGCACAGATTCATCCATCTGATGAAAATTTTCAGTTTCAGCCTATGAGGTTTTTAGCATTGGGGAACCAAACAGGAAATTTGAGGCTCTATTTTTACTCACCTGGTATGTCGAAATTTGATGCTTCTTTTCAAGCGCTCAACGCCACATGTGATCTTCCATTGGCGTGTAAGCCCTACGGAGTTTGTACATTGTCCGGAGCTTGTTCATGCATTCCACTCTTGCCCACCGAAAATGAGACGGGTACTGGTACTAGTACTACTGCTTCTGACTGCAGACGAGGAATTTCATCAGGAGGGTTTTGCAAAAAAGGGAAGAAAACGAAGGTGGAGATGCGTGAATTGAAGGGTGTTACTAGTGTTTTGAGAGGAGGTGCTAAGATTGTTAATGTGAGCAGAGAAGAATGTGGTAATTTGTGCTTCGAGGACTGTAATTGTACAGCAGCATTGTATTCTTCTGCAAAAGAGTGCTTTATATATGGAATGGTAATTGGTGTTAAACAGGTTGAGAATAAGGGAAGTGGATTATTGAGTTATATGGTGAAGGTTCCAAAGGGAGGTCATGGGGGTCATGGGAAGTCAAATTTGAAGAAATGGGTTTTGATCTTGGTAGGAGTGGTTGATGGGTTGATTATTCTTCTTGTTTTTGGAGGGCTTGGATATTACTTGGTAAGGAAGAGAAGAAGACACTCATTTGCCTAA

Protein Analysis

463

Amino Acids

51.13

Weight (kDa)

9.38

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 91 - 164 3.7e-09 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51710
fragaria_vesca FvH4_6g10470
malus_domestica MD04G1170300.v1.1 MD12G1184300.v1.1
prunus_persica Prupe.4G286300_v2.0.a1 Prupe.6G289100_v2.0.a1
pyrus_communis pycom12g17300
rosa_chinensis RchiOBHm_Chr3g0461371
rosa_laevigata RLG00000024937
rosa_multiflora Rmu_sc0002677.1_g000023
rosa_roxburghii Rroxscaffold_6G00418360 Rroxscaffold_6G00418660
rosa_rugosa Rorug03G0049700
rosa_samantha Rh3AG107800 Rh3BG111800 Rh3CG113600 Rh3DG113600
rosa_wichuraiana Rw3G009200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AccIII TCCGGA 1 cut(s) 884
AclWI GGATC 2 cut(s) 111, 124
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 7 cut(s) 571, 602, 715, 766, 803, 972, 1270
AfaI GTAC 6 cut(s) 200, 877, 934, 940, 946, 1126
AfeI AGCGCT 2 cut(s) 124, 825
AfiI CCNNNNNNNGG 1 cut(s) 1244
AflIII ACRYGT 1 cut(s) 836
AgsI TTSAA 9 cut(s) 276, 322, 545, 571, 607, 641, 821, 1033, 1276
AhlI ACTAGT 2 cut(s) 941, 1043
AjnI CCWGG 1 cut(s) 790
AluBI AGCT 3 cut(s) 7, 295, 890
AluI AGCT 3 cut(s) 7, 295, 890
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 469, 920
AlwI GGATC 2 cut(s) 111, 124
Aor13HI TCCGGA 1 cut(s) 884
Aor51HI AGCGCT 2 cut(s) 124, 825
AoxI GGCC 2 cut(s) 250, 325
ApaI GGGCCC 1 cut(s) 329
ApeKI GCWGC 2 cut(s) 4, 1130
ApoI RAATTY 7 cut(s) 571, 602, 715, 766, 803, 972, 1270
Asp700I GAANNNNTTC 1 cut(s) 717
AspLEI GCGC 2 cut(s) 125, 826
AspS9I GGNCC 3 cut(s) 325, 326, 647
AsuHPI GGTGA 3 cut(s) 19, 780, 1240
AvaII GGWCC 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 252
BamHI GGATCC 1 cut(s) 116
BanII GRGCYC 2 cut(s) 297, 329
BarI GAAGNNNNNNTAC 2 cut(s) 937, 969
BbsI GAAGAC 1 cut(s) 1378
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 16, 1142
BccI CCATC 3 cut(s) 339, 712, 1304
BceAI ACGGC 1 cut(s) 61
BciT130I CCWGG 1 cut(s) 792
BciVI GTATCC 1 cut(s) 68
BcoDI GTCTC 2 cut(s) 469, 920
BcuI ACTAGT 2 cut(s) 941, 1043
BfaI CTAG 6 cut(s) 398, 404, 416, 492, 942, 1044
BfmI CTRYAG 1 cut(s) 961
BfoI RGCGCY 2 cut(s) 126, 827
BfuI GTATCC 1 cut(s) 68
BisI GCNGC 2 cut(s) 5, 1131
BlsI GCNGC 2 cut(s) 6, 1132
BmcAI AGTACT 2 cut(s) 200, 946
Bme1390I CCNGG 1 cut(s) 792
Bme18I GGWCC 1 cut(s) 647
BmgT120I GGNCC 3 cut(s) 325, 326, 647
BmiI GGNNCC 6 cut(s) 118, 183, 327, 648, 755, 1236
BmrFI CCNGG 1 cut(s) 792
BmsI GCATC 4 cut(s) 52, 400, 799, 1011
BpiI GAAGAC 1 cut(s) 1378
BpuEI CTTGAG 1 cut(s) 311
BsaWI WCCGGW 1 cut(s) 884
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 1244
Bse1I ACTGG 2 cut(s) 92, 940
Bse3DI GCAATG 1 cut(s) 627
BseAI TCCGGA 1 cut(s) 884
BseBI CCWGG 1 cut(s) 792
BseGI GGATG 2 cut(s) 350, 700
BseLI CCNNNNNNNGG 1 cut(s) 1244
BseMI GCAATG 1 cut(s) 627
BseNI ACTGG 2 cut(s) 92, 940
BseRI GAGGAG 2 cut(s) 57, 1071
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 2 cut(s) 16, 1142
BseYI CCCAGC 1 cut(s) 548
BsgI GTGCAG 1 cut(s) 395
BshFI GGCC 2 cut(s) 252, 327
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 885
BslFI GGGAC 2 cut(s) 370, 483
BslI CCNNNNNNNGG 1 cut(s) 1244
BsmAI GTCTC 2 cut(s) 469, 920
BsmBI CGTCTC 1 cut(s) 920
BsmFI GGGAC 2 cut(s) 370, 483
BsmI GAATGC 1 cut(s) 900
BsnI GGCC 2 cut(s) 252, 327
Bsp120I GGGCCC 1 cut(s) 325
Bsp1286I GDGCHC 2 cut(s) 297, 329
Bsp13I TCCGGA 1 cut(s) 884
Bsp1407I TGTACA 2 cut(s) 875, 1124
Bsp143I GATC 3 cut(s) 116, 841, 1290
BspANI GGCC 2 cut(s) 252, 327
BspEI TCCGGA 1 cut(s) 884
BspLI GGNNCC 6 cut(s) 118, 183, 327, 648, 755, 1236
BspMAI CTGCAG 1 cut(s) 965
BspPI GGATC 2 cut(s) 111, 124
BsrDI GCAATG 1 cut(s) 627
BsrGI TGTACA 2 cut(s) 875, 1124
BsrI ACTGG 2 cut(s) 92, 940
BssMI GATC 3 cut(s) 116, 841, 1290
Bst2UI CCWGG 1 cut(s) 792
Bst4CI ACNGT 2 cut(s) 110, 1118
Bst6I CTCTTC 1 cut(s) 1358
BstAUI TGTACA 2 cut(s) 875, 1124
BstDEI CTNAG 2 cut(s) 479, 1065
BstF5I GGATG 2 cut(s) 350, 700
BstH2I RGCGCY 2 cut(s) 126, 827
BstHHI GCGC 2 cut(s) 125, 826
BstKTI GATC 3 cut(s) 119, 844, 1293
BstMAI GTCTC 2 cut(s) 469, 920
BstMBI GATC 3 cut(s) 116, 841, 1290
BstMWI GCNNNNNNNGC 1 cut(s) 830
BstNI CCWGG 1 cut(s) 792
BstNSI RCATGY 1 cut(s) 840
BstSCI CCNGG 1 cut(s) 790
BstSFI CTRYAG 1 cut(s) 961
BstSLI GKGCMC 1 cut(s) 329
BstV1I GCAGC 2 cut(s) 16, 1142
BstV2I GAAGAC 1 cut(s) 1378
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BsuI GTATCC 1 cut(s) 68
BsuRI GGCC 2 cut(s) 252, 327
BtsCI GGATG 2 cut(s) 350, 700
BtsIMutI CAGTG 2 cut(s) 99, 115
CfoI GCGC 2 cut(s) 125, 826
Cfr13I GGNCC 3 cut(s) 325, 326, 647
CsiI ACCWGGT 1 cut(s) 790
Csp6I GTAC 6 cut(s) 199, 876, 933, 939, 945, 1125
CviAII CATG 5 cut(s) 204, 837, 897, 1250, 1259
CviQI GTAC 6 cut(s) 199, 876, 933, 939, 945, 1125
DdeI CTNAG 2 cut(s) 479, 1065
DpnI GATC 3 cut(s) 118, 843, 1292
DpnII GATC 3 cut(s) 116, 841, 1290
DraI TTTAAA 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 1358
EarI CTCTTC 1 cut(s) 1358
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 2 cut(s) 297, 329
Eco47I GGWCC 1 cut(s) 647
Eco47III AGCGCT 2 cut(s) 124, 825
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 325
EcoRI GAATTC 2 cut(s) 571, 602
EcoRII CCWGG 1 cut(s) 790
EcoT22I ATGCAT 1 cut(s) 902
EcoT38I GRGCYC 2 cut(s) 297, 329
Esp3I CGTCTC 1 cut(s) 920
FaeI CATG 5 cut(s) 207, 840, 900, 1253, 1262
FalI AAGNNNNNCTT 6 cut(s) 207, 239, 343, 375, 472, 504
FaqI GGGAC 2 cut(s) 370, 483
FatI CATG 5 cut(s) 203, 836, 896, 1249, 1258
Fnu4HI GCNGC 2 cut(s) 5, 1131
FokI GGATG 2 cut(s) 357, 687
FriOI GRGCYC 2 cut(s) 297, 329
Fsp4HI GCNGC 2 cut(s) 5, 1131
FspBI CTAG 6 cut(s) 398, 404, 416, 492, 942, 1044
GlaI GCGC 2 cut(s) 124, 825
GluI GCNGC 2 cut(s) 5, 1131
GsaI CCCAGC 1 cut(s) 552
HaeII RGCGCY 2 cut(s) 126, 827
HaeIII GGCC 2 cut(s) 252, 327
HapII CCGG 1 cut(s) 885
HhaI GCGC 2 cut(s) 125, 826
Hin1II CATG 5 cut(s) 207, 840, 900, 1253, 1262
Hin6I GCGC 2 cut(s) 123, 824
HinP1I GCGC 2 cut(s) 123, 824
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HinfI GANTC 1 cut(s) 697
HpaII CCGG 1 cut(s) 885
HphI GGTGA 3 cut(s) 19, 780, 1240
Hpy166II GTNNAC 1 cut(s) 498
Hpy188I TCNGA 3 cut(s) 74, 709, 958
Hpy188III TCNNGA 5 cut(s) 290, 363, 536, 885, 981
Hpy8I GTNNAC 1 cut(s) 498
HpyAV CCTTC 4 cut(s) 316, 1006, 1027, 1225
HpyCH4III ACNGT 2 cut(s) 110, 1118
HpyCH4V TGCA 9 cut(s) 4, 214, 376, 413, 692, 900, 963, 993, 1148
HpyF10VI GCNNNNNNNGC 1 cut(s) 830
HpyF3I CTNAG 2 cut(s) 479, 1065
Hsp92II CATG 5 cut(s) 207, 840, 900, 1253, 1262
HspAI GCGC 2 cut(s) 123, 824
Kpn2I TCCGGA 1 cut(s) 884
Kzo9I GATC 3 cut(s) 116, 841, 1290
LmnI GCTCC 3 cut(s) 70, 212, 887
Lsp1109I GCAGC 2 cut(s) 16, 1142
LweI GCATC 4 cut(s) 52, 400, 799, 1011
MabI ACCWGGT 1 cut(s) 790
MaeI CTAG 6 cut(s) 398, 404, 416, 492, 942, 1044
MaeIII GTNAC 2 cut(s) 302, 1039
MalI GATC 3 cut(s) 118, 843, 1292
MboI GATC 3 cut(s) 116, 841, 1290
MfeI CAATTG 1 cut(s) 641
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 2 cut(s) 297, 329
MlsI TGGCCA 1 cut(s) 252
MluNI TGGCCA 1 cut(s) 252
MmeI TCCRAC 2 cut(s) 148, 473
Mox20I TGGCCA 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 902
MroI TCCGGA 1 cut(s) 884
MroXI GAANNNNTTC 1 cut(s) 717
MscI TGGCCA 1 cut(s) 252
MseI TTAA 4 cut(s) 162, 441, 1074, 1185
MslI CAYNNNNRTG 1 cut(s) 853
Msp20I TGGCCA 1 cut(s) 252
MspI CCGG 1 cut(s) 885
MspR9I CCNGG 1 cut(s) 792
MunI CAATTG 1 cut(s) 641
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 1 cut(s) 792
MwoI GCNNNNNNNGC 1 cut(s) 830
NdeII GATC 3 cut(s) 116, 841, 1290
NlaIII CATG 5 cut(s) 207, 840, 900, 1253, 1262
NlaIV GGNNCC 6 cut(s) 118, 183, 327, 648, 755, 1236
NmuCI GTSAC 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 902
NspI RCATGY 1 cut(s) 840
PciI ACATGT 1 cut(s) 836
PctI GAATGC 1 cut(s) 900
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 1 cut(s) 697
PkrI GCNGC 2 cut(s) 6, 1132
PscI ACATGT 1 cut(s) 836
PsiI TTATAA 1 cut(s) 675
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 790
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 1 cut(s) 790
PspN4I GGNNCC 6 cut(s) 118, 183, 327, 648, 755, 1236
PspOMI GGGCCC 1 cut(s) 325
PspPI GGNCC 3 cut(s) 325, 326, 647
PstI CTGCAG 1 cut(s) 965
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 6 cut(s) 200, 877, 934, 940, 946, 1126
RsaNI GTAC 6 cut(s) 199, 876, 933, 939, 945, 1125
RseI CAYNNNNRTG 1 cut(s) 853
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 4 cut(s) 162, 441, 1074, 1185
SatI GCNGC 2 cut(s) 5, 1131
Sau3AI GATC 3 cut(s) 116, 841, 1290
Sau96I GGNCC 3 cut(s) 325, 326, 647
ScaI AGTACT 2 cut(s) 200, 946
ScrFI CCNGG 1 cut(s) 792
SduI GDGCHC 2 cut(s) 297, 329
SexAI ACCWGGT 1 cut(s) 790
SfaNI GCATC 4 cut(s) 52, 400, 799, 1011
SfcI CTRYAG 1 cut(s) 961
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 853
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SpeI ACTAGT 2 cut(s) 941, 1043
SspMI CTAG 6 cut(s) 398, 404, 416, 492, 942, 1044
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 790
TaaI ACNGT 2 cut(s) 110, 1118
TaqI TCGA 4 cut(s) 53, 537, 800, 1110
TatI WGTACW 4 cut(s) 198, 875, 944, 1124
TfiI GAWTC 1 cut(s) 697
Tru1I TTAA 4 cut(s) 162, 441, 1074, 1185
Tru9I TTAA 4 cut(s) 162, 441, 1074, 1185
TscAI CASTG 2 cut(s) 99, 115
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 2 cut(s) 4, 1130
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 8 cut(s) 7, 159, 492, 513, 689, 726, 885, 966
TspGWI ACGGA 2 cut(s) 321, 882
TspRI CASTG 2 cut(s) 99, 115
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 7 cut(s) 571, 602, 715, 766, 803, 972, 1270
XceI RCATGY 1 cut(s) 840
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 6 cut(s) 398, 404, 416, 492, 942, 1044
ZrmI AGTACT 2 cut(s) 200, 946
Zsp2I ATGCAT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.