RLG00000024937

G-type lectin S-receptor-like serine threonine-protein kinase SD2-5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013054
Physical Location & Seq
Reverse (-)
40511039 .. 40512531
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024937

Sequence Viewer

Length: 1395 bp
ATGATGCTCTACTACCTGTTCATTTCCCTAACTCTTTTCGTCGCTCATGGCCACTCCAAGCCAGAGATTCATATTGGCTACAACCTCACTTTGGCAGTGCCTGTTGAGTACACCATGGGATTCATTGGCAGAGCTTTTCTAATGGATACCAATCTAACCATACCCAGTTTCAAAGCTGCATTGAGTGTTGAAGCTTTTAATGGGAAGTACTCATGCTCCTTTCAAGTCTTTCTTGGAAATGTGAAGGTATGGAATTCTGGCCATTACTCAAAGTTTTATACATCGAATGAATGCGTCCTTGAACTCACCAGTGATGGAGATTTAAGGCTGAAGGGTCCAAAAGACAGAGTGGGCTGGAGAACTGGGACTTCTGGACAAGGTGTGGAGAGGTTAGAGTTACTGAGGACAGGGAATCTAGTACTAGTTGATGGCTTGGACCGGATCAAATGGCAGAGTTTTAATTTTCCGACCGATGTGATGCTTTGGGGTCAGACACTTAGTGTGGCGTCACGCTTGACTTCATTTCCAAGCAATTCAAGTTCATACTACTCGATGGAAATTGAGCACACCAGGATTGCTCTCTACTTGAATTCCGGTAAGCAAAACTATTCGTATTGGGAATTTAAGCCTTCCAAGAACAGAAATATTGCTTTTATTCGATTGGGTCTACAAGGATTAGAGTTATACAGTGATAAAGGTAAGAAAATCGCGCAGATAAATCCATCTGAGGAAACAGTTCAGCCCCTGAGATTTTTGGCTTTGGGGAACCAAACAGGGAATTTGAGGCTTTATTTTTACTCACCCAGCTTGGAAAAATTTGATGCTTCTTTTCAAGCACTCAACACAACATGTGATCTTCCATTGGCTTGCAAGCCTTATGGCATTTGTACCTTGTCTGGTGCTTGTTCATGCATTCAAGTTTTGACAACGGAAAATGAGAGTAGTACTACTAGTACCACTTCTAATTGCATTGAAGGAGTTTCATCAGATGGGTTTTGTGGAAGAGGTAACGCAGAAATGCTTGAATTGGAGAGTGTCACTAGTGTGCTAAGGGGTGCTCCTAAAATAGTTAATGTAAGCAAAGAAGAATGTGGTAAATTGTGCTTGCAAGATTGTAAGTGTGCATCTGCATTACATAACTCTGAAGATTGCTTTCTCTATGGAATGGTGATTGGGGTTAAACAGGTTGAAAGGGAAAGTGGGTTGAGTTACATGGTTAAGGTTCCAAAGGGATCAAGTCATGGGGGTCATGGAAAGTCTAATGTGAAAAAATGGGTGTTGATATTGGTAGGAGTTGTTGATGGGGTGATAATTCTTGTTCTCTTTGGAGGTCTTGGCTACTACTTGATTAGAAAAAGAAGAAGACATTCTTTGCCTAATGGCCATAACAATTGA

Protein Analysis

465

Amino Acids

51.39

Weight (kDa)

8.71

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 94 - 170 3.1e-10 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51710
fragaria_vesca FvH4_6g10470
malus_domestica MD04G1170300.v1.1 MD12G1184300.v1.1
prunus_persica Prupe.4G286300_v2.0.a1 Prupe.6G289100_v2.0.a1
pyrus_communis pycom12g17300
rosa_chinensis RchiOBHm_Chr3g0461371
rosa_laevigata RLG00000024937
rosa_multiflora Rmu_sc0002677.1_g000023
rosa_roxburghii Rroxscaffold_6G00418360 Rroxscaffold_6G00418660
rosa_rugosa Rorug03G0049700
rosa_samantha Rh3AG107800 Rh3BG111800 Rh3CG113600 Rh3DG113600
rosa_wichuraiana Rw3G009200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 667
AccII CGCG 1 cut(s) 710
AclWI GGATC 2 cut(s) 449, 1240
AcoI YGGCCR 3 cut(s) 49, 259, 1381
AcsI RAATTY 5 cut(s) 253, 589, 620, 778, 815
AcuI CTGAAG 2 cut(s) 350, 1164
AcyI GRCGYC 1 cut(s) 506
AdeI CACNNNGTG 1 cut(s) 500
AfaI GTAC 6 cut(s) 110, 209, 420, 889, 946, 955
AfiI CCNNNNNNNGG 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 848
AhlI ACTAGT 3 cut(s) 421, 950, 1040
AjnI CCWGG 1 cut(s) 569
AleI CACNNNNGTG 1 cut(s) 1043
AluBI AGCT 4 cut(s) 134, 176, 194, 807
AluI AGCT 4 cut(s) 134, 176, 194, 807
Alw21I GWGCWC 2 cut(s) 567, 1060
AlwI GGATC 2 cut(s) 449, 1240
AlwNI CAGNNNCTG 2 cut(s) 101, 745
AoxI GGCC 3 cut(s) 49, 259, 1381
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 5 cut(s) 253, 589, 620, 778, 815
Asp700I GAANNNNTTC 2 cut(s) 735, 1366
AspLEI GCGC 1 cut(s) 712
AspS9I GGNCC 2 cut(s) 335, 436
AsuHPI GGTGA 4 cut(s) 298, 792, 1180, 1318
AvaII GGWCC 2 cut(s) 335, 436
BalI TGGCCA 3 cut(s) 51, 261, 1383
BbsI GAAGAC 1 cut(s) 1369
Bbv12I GWGCWC 2 cut(s) 567, 1060
BbvI GCAGC 1 cut(s) 163
BccI CCATC 6 cut(s) 308, 422, 547, 730, 983, 1295
BciT130I CCWGG 1 cut(s) 571
BciVI GTATCC 1 cut(s) 139
BcuI ACTAGT 3 cut(s) 421, 950, 1040
BfaI CTAG 4 cut(s) 416, 422, 951, 1041
BfuI GTATCC 1 cut(s) 139
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
BmcAI AGTACT 3 cut(s) 209, 420, 946
Bme1390I CCNGG 1 cut(s) 571
Bme18I GGWCC 2 cut(s) 335, 436
BmgT120I GGNCC 2 cut(s) 335, 436
BmiI GGNNCC 3 cut(s) 336, 767, 1224
BmrFI CCNGG 1 cut(s) 571
BmrI ACTGGG 2 cut(s) 159, 372
BmsI GCATC 3 cut(s) 468, 811, 1133
BmuI ACTGGG 2 cut(s) 159, 372
BpiI GAAGAC 1 cut(s) 1369
BpmI CTGGAG 1 cut(s) 376
Bpu10I CCTNAGC 1 cut(s) 1049
BsaBI GATNNNNATC 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 114
BsaWI WCCGGW 2 cut(s) 438, 593
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse1I ACTGG 3 cut(s) 165, 309, 367
Bse8I GATNNNNATC 1 cut(s) 150
BseBI CCWGG 1 cut(s) 571
BseDI CCNNGG 1 cut(s) 114
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 3 cut(s) 392, 717, 737
BseNI ACTGG 3 cut(s) 165, 309, 367
BseXI GCAGC 1 cut(s) 163
BseYI CCCAGC 1 cut(s) 803
Bsh1236I CGCG 1 cut(s) 710
Bsh1285I CGRYCG 1 cut(s) 471
BshFI GGCC 3 cut(s) 51, 261, 1383
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 2 cut(s) 567, 1060
BsiSI CCGG 2 cut(s) 439, 594
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 91
BsmFI GGGAC 1 cut(s) 379
BsmI GAATGC 2 cut(s) 296, 912
BsnI GGCC 3 cut(s) 51, 261, 1383
Bsp1286I GDGCHC 2 cut(s) 567, 1060
Bsp143I GATC 3 cut(s) 441, 853, 1232
Bsp19I CCATGG 1 cut(s) 114
BspANI GGCC 3 cut(s) 51, 261, 1383
BspCNI CTCAG 3 cut(s) 393, 718, 738
BspFNI CGCG 1 cut(s) 710
BspLI GGNNCC 3 cut(s) 336, 767, 1224
BspPI GGATC 2 cut(s) 449, 1240
BsrI ACTGG 3 cut(s) 165, 309, 367
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 3 cut(s) 441, 853, 1232
BssNI GRCGYC 1 cut(s) 506
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 571
Bst4CI ACNGT 2 cut(s) 689, 736
Bst6I CTCTTC 1 cut(s) 997
BstACI GRCGYC 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 868, 872, 1106
BstDEI CTNAG 5 cut(s) 401, 497, 726, 746, 1049
BstDSI CCRYGG 1 cut(s) 114
BstFNI CGCG 1 cut(s) 710
BstHHI GCGC 1 cut(s) 712
BstKTI GATC 3 cut(s) 444, 856, 1235
BstMBI GATC 3 cut(s) 441, 853, 1232
BstMCI CGRYCG 1 cut(s) 471
BstNI CCWGG 1 cut(s) 571
BstNSI RCATGY 1 cut(s) 852
BstSCI CCNGG 1 cut(s) 569
BstUI CGCG 1 cut(s) 710
BstV1I GCAGC 1 cut(s) 163
BstV2I GAAGAC 1 cut(s) 1369
BsuI GTATCC 1 cut(s) 139
BsuRI GGCC 3 cut(s) 51, 261, 1383
BtgI CCRYGG 1 cut(s) 114
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 3 cut(s) 102, 316, 694
Cac8I GCNNGC 3 cut(s) 868, 872, 1106
CaiI CAGNNNCTG 2 cut(s) 101, 745
CfoI GCGC 1 cut(s) 712
Cfr13I GGNCC 2 cut(s) 335, 436
CseI GACGC 2 cut(s) 283, 495
Csp6I GTAC 6 cut(s) 109, 208, 419, 888, 945, 954
CviAII CATG 8 cut(s) 47, 115, 213, 849, 909, 1213, 1241, 1250
CviQI GTAC 6 cut(s) 109, 208, 419, 888, 945, 954
DdeI CTNAG 5 cut(s) 401, 497, 726, 746, 1049
DpnI GATC 3 cut(s) 443, 855, 1234
DpnII GATC 3 cut(s) 441, 853, 1232
DraIII CACNNNGTG 1 cut(s) 500
EaeI YGGCCR 3 cut(s) 49, 259, 1381
Eam1104I CTCTTC 1 cut(s) 997
EarI CTCTTC 1 cut(s) 997
Eco130I CCWWGG 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 335, 436
Eco57I CTGAAG 2 cut(s) 350, 1164
EcoRI GAATTC 2 cut(s) 253, 589
EcoRII CCWGG 1 cut(s) 569
EcoT14I CCWWGG 1 cut(s) 114
EcoT22I ATGCAT 1 cut(s) 914
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 8 cut(s) 50, 118, 216, 852, 912, 1216, 1244, 1253
FalI AAGNNNNNCTT 4 cut(s) 216, 248, 1354, 1386
FaqI GGGAC 1 cut(s) 379
FatI CATG 8 cut(s) 46, 114, 212, 848, 908, 1212, 1240, 1249
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 1 cut(s) 177
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 4 cut(s) 416, 422, 951, 1041
GlaI GCGC 1 cut(s) 711
GluI GCNGC 1 cut(s) 177
GsaI CCCAGC 1 cut(s) 807
GsuI CTGGAG 1 cut(s) 376
HaeIII GGCC 3 cut(s) 51, 261, 1383
HapII CCGG 2 cut(s) 439, 594
HgaI GACGC 2 cut(s) 283, 495
HhaI GCGC 1 cut(s) 712
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 8 cut(s) 50, 118, 216, 852, 912, 1216, 1244, 1253
Hin6I GCGC 1 cut(s) 710
HinP1I GCGC 1 cut(s) 710
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 3 cut(s) 67, 120, 412
HpaII CCGG 2 cut(s) 439, 594
HphI GGTGA 4 cut(s) 298, 792, 1180, 1318
Hpy166II GTNNAC 2 cut(s) 111, 668
Hpy188I TCNGA 5 cut(s) 468, 492, 727, 988, 1144
Hpy188III TCNNGA 1 cut(s) 372
Hpy8I GTNNAC 2 cut(s) 111, 668
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 4 cut(s) 238, 325, 639, 968
HpyCH4III ACNGT 2 cut(s) 689, 736
HpyCH4V TGCA 7 cut(s) 179, 870, 912, 969, 1108, 1124, 1130
HpyF3I CTNAG 5 cut(s) 401, 497, 726, 746, 1049
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 8 cut(s) 50, 118, 216, 852, 912, 1216, 1244, 1253
HspAI GCGC 1 cut(s) 710
Kzo9I GATC 3 cut(s) 441, 853, 1232
LmnI GCTCC 2 cut(s) 221, 1063
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 3 cut(s) 468, 811, 1133
MaeI CTAG 4 cut(s) 416, 422, 951, 1041
MaeIII GTNAC 5 cut(s) 396, 507, 1007, 1036, 1208
MalI GATC 3 cut(s) 443, 855, 1234
MboI GATC 3 cut(s) 441, 853, 1232
MboII GAAGA 6 cut(s) 848, 1014, 1097, 1157, 1371, 1374
MfeI CAATTG 1 cut(s) 1390
MhlI GDGCHC 2 cut(s) 567, 1060
MlsI TGGCCA 3 cut(s) 51, 261, 1383
MluNI TGGCCA 3 cut(s) 51, 261, 1383
MmeI TCCRAC 1 cut(s) 491
MnlI CCTC 7 cut(s) 95, 381, 396, 721, 777, 998, 1322
Mox20I TGGCCA 3 cut(s) 51, 261, 1383
Mph1103I ATGCAT 1 cut(s) 914
MroXI GAANNNNTTC 2 cut(s) 735, 1366
MscI TGGCCA 3 cut(s) 51, 261, 1383
MseI TTAA 7 cut(s) 198, 323, 459, 624, 1071, 1179, 1218
MslI CAYNNNNRTG 1 cut(s) 1043
Msp20I TGGCCA 3 cut(s) 51, 261, 1383
MspI CCGG 2 cut(s) 439, 594
MspR9I CCNGG 1 cut(s) 571
MunI CAATTG 1 cut(s) 1390
Mva1269I GAATGC 2 cut(s) 296, 912
MvaI CCWGG 1 cut(s) 571
MvnI CGCG 1 cut(s) 710
NcoI CCATGG 1 cut(s) 114
NdeII GATC 3 cut(s) 441, 853, 1232
NlaIII CATG 8 cut(s) 50, 118, 216, 852, 912, 1216, 1244, 1253
NlaIV GGNNCC 3 cut(s) 336, 767, 1224
NmuCI GTSAC 2 cut(s) 507, 1036
NsiI ATGCAT 1 cut(s) 914
NspI RCATGY 1 cut(s) 852
OliI CACNNNNGTG 1 cut(s) 1043
PciI ACATGT 1 cut(s) 848
PctI GAATGC 2 cut(s) 296, 912
PdmI GAANNNNTTC 2 cut(s) 735, 1366
PfeI GAWTC 3 cut(s) 67, 120, 412
PkrI GCNGC 1 cut(s) 178
PscI ACATGT 1 cut(s) 848
Psp6I CCWGG 1 cut(s) 569
PspFI CCCAGC 1 cut(s) 803
PspGI CCWGG 1 cut(s) 569
PspN4I GGNNCC 3 cut(s) 336, 767, 1224
PspPI GGNCC 2 cut(s) 335, 436
PstNI CAGNNNCTG 2 cut(s) 101, 745
RsaI GTAC 6 cut(s) 110, 209, 420, 889, 946, 955
RsaNI GTAC 6 cut(s) 109, 208, 419, 888, 945, 954
RseI CAYNNNNRTG 1 cut(s) 1043
SaqAI TTAA 7 cut(s) 198, 323, 459, 624, 1071, 1179, 1218
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 3 cut(s) 441, 853, 1232
Sau96I GGNCC 2 cut(s) 335, 436
ScaI AGTACT 3 cut(s) 209, 420, 946
ScrFI CCNGG 1 cut(s) 571
SduI GDGCHC 2 cut(s) 567, 1060
SfaNI GCATC 3 cut(s) 468, 811, 1133
SinI GGWCC 2 cut(s) 335, 436
SmiMI CAYNNNNRTG 1 cut(s) 1043
SpeI ACTAGT 3 cut(s) 421, 950, 1040
SspI AATATT 1 cut(s) 646
SspMI CTAG 4 cut(s) 416, 422, 951, 1041
StyD4I CCNGG 1 cut(s) 569
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 2 cut(s) 689, 736
TaqI TCGA 3 cut(s) 284, 551, 658
TaqII GACCGA 1 cut(s) 485
TatI WGTACW 4 cut(s) 108, 207, 418, 944
TfiI GAWTC 3 cut(s) 67, 120, 412
Tru1I TTAA 7 cut(s) 198, 323, 459, 624, 1071, 1179, 1218
Tru9I TTAA 7 cut(s) 198, 323, 459, 624, 1071, 1179, 1218
TscAI CASTG 3 cut(s) 102, 316, 694
TseFI GTSAC 2 cut(s) 507, 1036
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 2 cut(s) 507, 1036
TspDTI ATGAA 8 cut(s) 10, 59, 112, 303, 510, 531, 897, 972
TspGWI ACGGA 1 cut(s) 944
TspRI CASTG 3 cut(s) 102, 316, 694
VpaK11BI GGWCC 2 cut(s) 335, 436
XapI RAATTY 5 cut(s) 253, 589, 620, 778, 815
XceI RCATGY 1 cut(s) 852
XmiI GTMKAC 1 cut(s) 667
XmnI GAANNNNTTC 2 cut(s) 735, 1366
XspI CTAG 4 cut(s) 416, 422, 951, 1041
ZrmI AGTACT 3 cut(s) 209, 420, 946
Zsp2I ATGCAT 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.