MD12G1190200.v1.1

High mobility group B protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011345
Physical Location & Seq
Reverse (-)
27309143 .. 27310175
1033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1190200.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCAGGCGCTTCAATCTTCCGTCGCCGGAATCGCCGGGAACCAGATTGTTCGGCCTAAAAGTGGCCGGACGCCGCTCCAGCCAAAGAAAGCTCCGGTGACTTCGACAAACTCCGATCTCAAAATCAAGCCGGTTCACCAATGGATTGGCGTTGGCGATGATTCAAATAAGGAGAACCGTCCAATGTATGCGACTCCGGTGAAGATTGAGGCGATGGACGCGTCTCTGGCAGAGGAGCTGAGCTCGATCAGGAAGAAGATGGAGAGGATGAAATCGGACAGAGAGAAGACCGAGAAGATGCTTAAGGAGAGGGATATGGTGATGGAAATACAGATGAAGGAGCTCGAAAACAGAGGGCAGATCCAGAAGATGCTCGAGACCGAGCTCGATCGGATTTACCGATTGAACCAGCTCCATGTTCAATCAATTAAAGTATCGCCGATTCGATCGCTCAGGGAGAAGGAGGAAGAAAAGAAGGCCGATGAATCGCCATCTCAGGAAGTGGAGGTGGAAGCGGAGGAGGATATGGAGGAATCTGTGGATGAAAACTCACCGCAGAAGCCGGAGAGTTGTGCTGCTTCCAATTCCGAAATTGTTACAGAGGAAAATAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

206

Amino Acids

23.35

Weight (kDa)

5.29

Isoelectric Point (pI)

60.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01910 AT5G01910
fragaria_vesca FvH4_6g09670
malus_domestica MD04G1175800.v1.1 MD05G1222900.v1.1 MD12G1190200.v1.1
prunus_persica Prupe.6G295300_v2.0.a1
pyrus_communis pycom04g15510 pycom12g17750
rosa_chinensis RchiOBHm_Chr3g0460321
rosa_laevigata RLG00000025024
rosa_multiflora Rmu_sc0000449.1_g000011 Rmu_sc0010030.1_g000003
rosa_roxburghii Rroxscaffold_6G00419640
rosa_rugosa Rorug03G0041600
rosa_samantha Rh3AG099400 Rh3BG103400 Rh3CG105000 Rh3DG104700
rosa_wichuraiana Rw3G008450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 76
AccII CGCG 1 cut(s) 221
AciI CCGC 3 cut(s) 74, 515, 554
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 1 cut(s) 64
AcyI GRCGYC 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 62
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 4 cut(s) 14, 165, 406, 422
AluBI AGCT 6 cut(s) 92, 238, 243, 343, 385, 412
AluI AGCT 6 cut(s) 92, 238, 243, 343, 385, 412
Alw21I GWGCWC 3 cut(s) 245, 345, 387
Alw26I GTCTC 2 cut(s) 228, 371
AlwI GGATC 1 cut(s) 355
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 3 cut(s) 53, 64, 477
ApeKI GCWGC 1 cut(s) 575
AspLEI GCGC 1 cut(s) 10
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 5 cut(s) 109, 128, 211, 331, 543
AvaI CYCGRG 1 cut(s) 374
BanII GRGCYC 3 cut(s) 245, 345, 387
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 3 cut(s) 245, 345, 387
BbvI GCAGC 1 cut(s) 562
BccI CCATC 4 cut(s) 208, 253, 316, 499
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 2 cut(s) 228, 371
BfoI RGCGCY 1 cut(s) 11
BfrI CTTAAG 1 cut(s) 302
BisI GCNGC 2 cut(s) 74, 576
BlpI GCTNAGC 1 cut(s) 239
BlsI GCNGC 2 cut(s) 75, 577
Bme1390I CCNGG 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 41
BmrFI CCNGG 1 cut(s) 37
BmsI GCATC 2 cut(s) 288, 360
BpiI GAAGAC 1 cut(s) 293
BplI GAGNNNNNCTC 2 cut(s) 227, 259
BpmI CTGGAG 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 452
Bpu1102I GCTNAGC 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 37
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 371
BsaWI WCCGGW 2 cut(s) 94, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse118I RCCGGY 1 cut(s) 130
BseGI GGATG 2 cut(s) 273, 547
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 3 cut(s) 230, 466, 509
BseRI GAGGAG 2 cut(s) 248, 533
BseXI GCAGC 1 cut(s) 562
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 2 cut(s) 391, 449
BshFI GGCC 3 cut(s) 55, 66, 479
BsiEI CGRYCG 2 cut(s) 391, 449
BsiHKAI GWGCWC 3 cut(s) 245, 345, 387
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 7 cut(s) 27, 36, 67, 95, 131, 197, 563
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 2 cut(s) 228, 371
BsmBI CGTCTC 1 cut(s) 228
BsnI GGCC 3 cut(s) 55, 66, 479
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 245, 345, 387
Bsp143I GATC 5 cut(s) 115, 246, 360, 388, 446
Bsp1720I GCTNAGC 1 cut(s) 239
BspACI CCGC 3 cut(s) 74, 515, 554
BspANI GGCC 3 cut(s) 55, 66, 479
BspCNI CTCAG 3 cut(s) 231, 465, 508
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 41
BspPI GGATC 1 cut(s) 355
BspTI CTTAAG 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 130
BssMI GATC 5 cut(s) 115, 246, 360, 388, 446
BssNI GRCGYC 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 179
BstACI GRCGYC 1 cut(s) 71
BstAFI CTTAAG 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 239, 452, 495
BstF5I GGATG 2 cut(s) 273, 547
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 5 cut(s) 118, 249, 363, 391, 449
BstMAI GTCTC 2 cut(s) 228, 371
BstMBI GATC 5 cut(s) 115, 246, 360, 388, 446
BstMCI CGRYCG 2 cut(s) 391, 449
BstMWI GCNNNNNNNGC 4 cut(s) 32, 79, 218, 227
BstSCI CCNGG 1 cut(s) 35
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 562
BstV2I GAAGAC 1 cut(s) 293
BstX2I RGATCY 1 cut(s) 360
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 1 cut(s) 360
BsuRI GGCC 3 cut(s) 55, 66, 479
BtgZI GCGATG 2 cut(s) 171, 227
BtsCI GGATG 2 cut(s) 273, 547
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 130
CseI GACGC 3 cut(s) 79, 210, 227
CviAII CATG 1 cut(s) 416
DdeI CTNAG 3 cut(s) 239, 452, 495
DpnI GATC 5 cut(s) 117, 248, 362, 390, 448
DpnII GATC 5 cut(s) 115, 246, 360, 388, 446
EaeI YGGCCR 1 cut(s) 64
Ecl136II GAGCTC 3 cut(s) 243, 343, 385
Eco24I GRGCYC 3 cut(s) 245, 345, 387
Eco31I GGTCTC 1 cut(s) 371
Eco53kI GAGCTC 3 cut(s) 243, 343, 385
Eco88I CYCGRG 1 cut(s) 374
EcoICRI GAGCTC 3 cut(s) 243, 343, 385
EcoT38I GRGCYC 3 cut(s) 245, 345, 387
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 1 cut(s) 419
FaiI YATR 4 cut(s) 189, 317, 417, 527
FatI CATG 1 cut(s) 415
Fnu4HI GCNGC 2 cut(s) 74, 576
FokI GGATG 2 cut(s) 280, 554
FriOI GRGCYC 3 cut(s) 245, 345, 387
Fsp4HI GCNGC 2 cut(s) 74, 576
GlaI GCGC 1 cut(s) 9
GluI GCNGC 2 cut(s) 74, 576
GsuI CTGGAG 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 55, 66, 479
HapII CCGG 7 cut(s) 27, 36, 67, 95, 131, 197, 563
HgaI GACGC 3 cut(s) 79, 210, 227
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 1 cut(s) 419
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 6 cut(s) 30, 161, 193, 442, 485, 533
HpaII CCGG 7 cut(s) 27, 36, 67, 95, 131, 197, 563
HphI GGTGA 5 cut(s) 109, 128, 211, 331, 543
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 4 cut(s) 115, 277, 393, 589
Hpy188III TCNNGA 4 cut(s) 250, 364, 376, 497
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 331, 454, 469
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 79, 218, 227
HpyF3I CTNAG 3 cut(s) 239, 452, 495
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 1 cut(s) 419
HspAI GCGC 1 cut(s) 8
Kzo9I GATC 5 cut(s) 115, 246, 360, 388, 446
LmnI GCTCC 5 cut(s) 81, 97, 235, 340, 417
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 2 cut(s) 288, 360
MaeIII GTNAC 2 cut(s) 97, 595
MalI GATC 5 cut(s) 117, 248, 362, 390, 448
MbiI CCGCTC 1 cut(s) 76
MboI GATC 5 cut(s) 115, 246, 360, 388, 446
MboII GAAGA 8 cut(s) 9, 214, 265, 268, 298, 307, 379, 479
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 3 cut(s) 245, 345, 387
MluCI AATT 3 cut(s) 426, 583, 591
MluI ACGCGT 1 cut(s) 219
MlyI GAGTC 1 cut(s) 187
MseI TTAA 2 cut(s) 303, 429
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 7 cut(s) 27, 36, 67, 95, 131, 197, 563
MspR9I CCNGG 1 cut(s) 37
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 32, 79, 218, 227
NciI CCSGG 1 cut(s) 37
NdeII GATC 5 cut(s) 115, 246, 360, 388, 446
NlaIII CATG 1 cut(s) 419
NlaIV GGNNCC 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 397
PfeI GAWTC 5 cut(s) 30, 161, 442, 485, 533
PkrI GCNGC 2 cut(s) 75, 577
Ple19I CGATCG 2 cut(s) 391, 449
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Psp124BI GAGCTC 3 cut(s) 245, 345, 387
PspN4I GGNNCC 1 cut(s) 41
PsuI RGATCY 1 cut(s) 360
PvuI CGATCG 2 cut(s) 391, 449
SacI GAGCTC 3 cut(s) 245, 345, 387
SaqAI TTAA 2 cut(s) 303, 429
SatI GCNGC 2 cut(s) 74, 576
Sau3AI GATC 5 cut(s) 115, 246, 360, 388, 446
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 37
SduI GDGCHC 3 cut(s) 245, 345, 387
SetI ASST 7 cut(s) 94, 240, 245, 345, 387, 414, 510
SfaNI GCATC 2 cut(s) 288, 360
Sfr274I CTCGAG 1 cut(s) 374
SlaI CTCGAG 1 cut(s) 374
SmlI CTYRAG 2 cut(s) 302, 374
SmoI CTYRAG 2 cut(s) 302, 374
Sse9I AATT 3 cut(s) 426, 583, 591
SsiI CCGC 3 cut(s) 74, 515, 554
SstI GAGCTC 3 cut(s) 245, 345, 387
StyD4I CCNGG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 6 cut(s) 104, 245, 345, 375, 387, 445
TaqII GACCGA 2 cut(s) 305, 395
TasI AATT 3 cut(s) 426, 583, 591
TauI GCSGC 1 cut(s) 76
TfiI GAWTC 5 cut(s) 30, 161, 442, 485, 533
Tru1I TTAA 2 cut(s) 303, 429
Tru9I TTAA 2 cut(s) 303, 429
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 4 cut(s) 284, 350, 498, 558
TspGWI ACGGA 1 cut(s) 10
Vha464I CTTAAG 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.