MD12G1199800.v1.1

G patch domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010106
Physical Location & Seq
Forward (+)
28074284 .. 28078228
3945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1199800.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGAGGATGCCAGAATTTCTCCTTAAAGGAAAGTGTTATTCATACACTAGGTCAGAAAACAGAGCCGGTTCAAGATCCGGAGAGCATCTTTGGTTCTTTCATTCGCCAGCAATTTTTGCTTTCTGCGTCAATCCAGGTAACATCATGGCCTACCGGTATAACGGTAGACAGGAAACAGGCACCCGAGGCAACCCGCAACATCAGAAAGAGCAGGCATATCAGAGTTCACTAGTTGATGATCTAGCAGACGAATTTCGATTGCCGATTAATCAAAGGCCCACAGAAAATGTTGATCTGGAGAATGTGGAACAAGCATCATTGGACACACACATAGCATCATCTAATGTTGGTTTTAGGCTTCTCCAAAAGATGGGGTGGAAGGGGAAGGGGCTTGGGAAGGATGAGCAAGGAATTACTGAGCCAATAAAATCTGGGATTAGAGACCCAAAATTAGGAGTTGGAAAACAAGAGGAAGATGATTATTATACAGCAGAAGAAAATATCCAGAGAAGAAAACTCGATGTGGAATTAGAAGAGACCGAGGAAAGTGCAAAGAAGCGGGAGGTATTAGCAGAACGTGAGCAGAAAATCCAAACCGAGGTGAAAGAAATACGCAAGGTGTTCTACTGTGAACTCTGCAGCAAACAATACAAGTTGGCCGTGGAATTTGAAGCTCACTTAAGCTCATATGATCACAATCACAGAAAGCGTTTTAAAGAAATGAAAGAAATGCACGGTTCCAGTAGTCGGGATGATCGCCAAAAACGAGAGCAACAGCGTCAGGAGAAGGAAATGGCAAAGTTTGTTCAGATTGCGGATGCTCGTAAGCAGCAGCAGCAGTTACAACAGCAACAAGAAGAATCTGGGCCTGCCCCAGTTTCCACTGAACTGAAAAGTGCTACTACACTTGCAGATCAGGATCAACGGAAGGCTTTGAAATTTGGGTTTTCTTCTAAAGGTGCAACCTCTAAGCCTAAGATCTCGTTTGGTAGTGCTGCAAAGAAGCCAAAAGTCGGCGTGGCCTCAGTATTCGGCAATGATAGCGATGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G-patch zf-C2H2_jaz

Protein Analysis

352

Amino Acids

40.2

Weight (kDa)

8.16

Isoelectric Point (pI)

52.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch PF01585 114 - 154 7.4e-16 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 370
AccI GTMKAC 1 cut(s) 166
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 3 cut(s) 194, 559, 815
AclWI GGATC 2 cut(s) 69, 927
AcoI YGGCCR 1 cut(s) 657
AcsI RAATTY 4 cut(s) 14, 251, 665, 938
AfiI CCNNNNNNNGG 5 cut(s) 370, 452, 598, 747, 1013
AflII CTTAAG 1 cut(s) 679
AgeI ACCGGT 1 cut(s) 153
AgsI TTSAA 3 cut(s) 72, 671, 937
AhlI ACTAGT 1 cut(s) 229
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 2 cut(s) 674, 684
AluI AGCT 2 cut(s) 674, 684
Alw26I GTCTC 2 cut(s) 435, 530
AlwI GGATC 2 cut(s) 69, 927
Ama87I CYCGRG 1 cut(s) 183
Aor13HI TCCGGA 1 cut(s) 77
AoxI GGCC 5 cut(s) 147, 275, 657, 866, 1020
ApeKI GCWGC 5 cut(s) 639, 829, 832, 835, 995
ApoI RAATTY 4 cut(s) 14, 251, 665, 938
AseI ATTAAT 1 cut(s) 267
AsiGI ACCGGT 1 cut(s) 153
AspS9I GGNCC 2 cut(s) 276, 866
AsuHPI GGTGA 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 183
BanI GGYRCC 1 cut(s) 179
BbvI GCAGC 5 cut(s) 651, 841, 844, 847, 982
BccI CCATC 1 cut(s) 364
BceAI ACGGC 1 cut(s) 644
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BciT130I CCWGG 1 cut(s) 135
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 2 cut(s) 435, 530
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 3 cut(s) 48, 230, 242
BfmI CTRYAG 1 cut(s) 637
BfrI CTTAAG 1 cut(s) 679
BglII AGATCT 1 cut(s) 978
BisI GCNGC 5 cut(s) 640, 830, 833, 836, 996
BlsI GCNGC 5 cut(s) 641, 831, 834, 837, 997
Bme1390I CCNGG 1 cut(s) 135
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 276, 866
BmiI GGNNCC 2 cut(s) 181, 739
BmrFI CCNGG 1 cut(s) 135
BmrI ACTGGG 1 cut(s) 869
BmsI GCATC 4 cut(s) 94, 323, 344, 808
BmuI ACTGGG 1 cut(s) 869
BpmI CTGGAG 1 cut(s) 317
BsaBI GATNNNNATC 2 cut(s) 696, 918
BsaI GGTCTC 2 cut(s) 435, 530
BsaJI CCNNGG 4 cut(s) 184, 540, 597, 660
BsaWI WCCGGW 2 cut(s) 77, 153
Bsc4I CCNNNNNNNGG 5 cut(s) 370, 452, 598, 747, 1013
Bse118I RCCGGY 2 cut(s) 65, 153
Bse1I ACTGG 2 cut(s) 741, 875
Bse3DI GCAATG 1 cut(s) 1042
Bse8I GATNNNNATC 2 cut(s) 696, 918
BseAI TCCGGA 1 cut(s) 77
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 4 cut(s) 184, 540, 597, 660
BseGI GGATG 4 cut(s) 12, 406, 757, 823
BseJI GATNNNNATC 2 cut(s) 696, 918
BseLI CCNNNNNNNGG 5 cut(s) 370, 452, 598, 747, 1013
BseMI GCAATG 1 cut(s) 1042
BseMII CTCAG 2 cut(s) 408, 1038
BseNI ACTGG 2 cut(s) 741, 875
BseXI GCAGC 5 cut(s) 651, 841, 844, 847, 982
BshFI GGCC 5 cut(s) 149, 277, 659, 868, 1022
BshNI GGYRCC 1 cut(s) 179
BshTI ACCGGT 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 3 cut(s) 66, 78, 154
BslI CCNNNNNNNGG 5 cut(s) 370, 452, 598, 747, 1013
BsmAI GTCTC 2 cut(s) 435, 530
BsnI GGCC 5 cut(s) 149, 277, 659, 868, 1022
Bso31I GGTCTC 2 cut(s) 435, 530
BsoBI CYCGRG 1 cut(s) 183
Bsp13I TCCGGA 1 cut(s) 77
Bsp143I GATC 8 cut(s) 74, 238, 292, 691, 754, 913, 919, 978
BspACI CCGC 3 cut(s) 194, 559, 815
BspANI GGCC 5 cut(s) 149, 277, 659, 868, 1022
BspCNI CTCAG 2 cut(s) 409, 1037
BspEI TCCGGA 1 cut(s) 77
BspLI GGNNCC 2 cut(s) 181, 739
BspMAI CTGCAG 1 cut(s) 641
BspPI GGATC 2 cut(s) 69, 927
BspT107I GGYRCC 1 cut(s) 179
BspTI CTTAAG 1 cut(s) 679
BspTNI GGTCTC 2 cut(s) 435, 530
BsrDI GCAATG 1 cut(s) 1042
BsrFI RCCGGY 2 cut(s) 65, 153
BsrI ACTGG 2 cut(s) 741, 875
BssAI RCCGGY 2 cut(s) 65, 153
BssECI CCNNGG 4 cut(s) 184, 540, 597, 660
BssMI GATC 8 cut(s) 74, 238, 292, 691, 754, 913, 919, 978
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 3 cut(s) 164, 629, 737
Bst6I CTCTTC 1 cut(s) 528
BstAFI CTTAAG 1 cut(s) 679
BstAPI GCANNNNNTGC 1 cut(s) 116
BstC8I GCNNGC 3 cut(s) 108, 213, 870
BstDEI CTNAG 4 cut(s) 417, 969, 975, 1024
BstDSI CCRYGG 1 cut(s) 660
BstF5I GGATG 4 cut(s) 12, 406, 757, 823
BstKTI GATC 8 cut(s) 77, 241, 295, 694, 757, 916, 922, 981
BstMAI GTCTC 2 cut(s) 435, 530
BstMBI GATC 8 cut(s) 74, 238, 292, 691, 754, 913, 919, 978
BstMWI GCNNNNNNNGC 4 cut(s) 116, 186, 835, 1041
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 637
BstV1I GCAGC 5 cut(s) 651, 841, 844, 847, 982
BstX2I RGATCY 2 cut(s) 74, 978
BstYI RGATCY 2 cut(s) 74, 978
BsuRI GGCC 5 cut(s) 149, 277, 659, 868, 1022
BtgI CCRYGG 1 cut(s) 660
BtsCI GGATG 4 cut(s) 12, 406, 757, 823
BtsIMutI CAGTG 1 cut(s) 882
Cac8I GCNNGC 3 cut(s) 108, 213, 870
Cfr10I RCCGGY 2 cut(s) 65, 153
Cfr13I GGNCC 2 cut(s) 276, 866
CseI GACGC 2 cut(s) 115, 767
CspAI ACCGGT 1 cut(s) 153
CviAII CATG 1 cut(s) 145
DdeI CTNAG 4 cut(s) 417, 969, 975, 1024
DpnI GATC 8 cut(s) 76, 240, 294, 693, 756, 915, 921, 980
DpnII GATC 8 cut(s) 74, 238, 292, 691, 754, 913, 919, 978
DraI TTTAAA 1 cut(s) 715
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco31I GGTCTC 2 cut(s) 435, 530
Eco88I CYCGRG 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 133
FaeI CATG 1 cut(s) 148
FaiI YATR 8 cut(s) 43, 146, 159, 217, 332, 486, 688, 690
FatI CATG 1 cut(s) 144
FauI CCCGC 2 cut(s) 201, 552
FauNDI CATATG 1 cut(s) 688
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 1 cut(s) 166
Fnu4HI GCNGC 5 cut(s) 640, 830, 833, 836, 996
FokI GGATG 4 cut(s) 19, 413, 764, 830
Fsp4HI GCNGC 5 cut(s) 640, 830, 833, 836, 996
FspBI CTAG 3 cut(s) 48, 230, 242
GluI GCNGC 5 cut(s) 640, 830, 833, 836, 996
GsuI CTGGAG 1 cut(s) 317
HaeIII GGCC 5 cut(s) 149, 277, 659, 868, 1022
HapII CCGG 3 cut(s) 66, 78, 154
HgaI GACGC 2 cut(s) 115, 767
Hin1II CATG 1 cut(s) 148
HinfI GANTC 1 cut(s) 860
HpaII CCGG 3 cut(s) 66, 78, 154
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 3 cut(s) 167, 227, 632
Hpy188I TCNGA 4 cut(s) 55, 204, 222, 810
Hpy188III TCNNGA 7 cut(s) 72, 78, 296, 505, 749, 782, 917
Hpy8I GTNNAC 3 cut(s) 167, 227, 632
HpyAV CCTTC 5 cut(s) 373, 379, 391, 781, 922
HpyCH4III ACNGT 3 cut(s) 164, 629, 737
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 6 cut(s) 551, 639, 733, 911, 962, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 186, 835, 1041
HpyF3I CTNAG 4 cut(s) 417, 969, 975, 1024
HpySE526I ACGT 1 cut(s) 577
Hsp92II CATG 1 cut(s) 148
Kpn2I TCCGGA 1 cut(s) 77
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 8 cut(s) 74, 238, 292, 691, 754, 913, 919, 978
Lsp1109I GCAGC 5 cut(s) 651, 841, 844, 847, 982
LweI GCATC 4 cut(s) 94, 323, 344, 808
MaeI CTAG 3 cut(s) 48, 230, 242
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 2 cut(s) 137, 840
MalI GATC 8 cut(s) 76, 240, 294, 693, 756, 915, 921, 980
MboI GATC 8 cut(s) 74, 238, 292, 691, 754, 913, 919, 978
MboII GAAGA 6 cut(s) 485, 506, 522, 545, 869, 942
MflI RGATCY 2 cut(s) 74, 978
MluCI AATT 8 cut(s) 14, 111, 251, 411, 449, 527, 665, 938
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 7 cut(s) 179, 463, 535, 556, 592, 976, 1033
MroI TCCGGA 1 cut(s) 77
MseI TTAA 4 cut(s) 24, 267, 680, 714
MspCI CTTAAG 1 cut(s) 679
MspI CCGG 3 cut(s) 66, 78, 154
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 4 cut(s) 116, 186, 835, 1041
NdeI CATATG 1 cut(s) 688
NdeII GATC 8 cut(s) 74, 238, 292, 691, 754, 913, 919, 978
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 2 cut(s) 181, 739
PcsI WCGNNNNNNNCGW 1 cut(s) 763
PfeI GAWTC 1 cut(s) 860
PflMI CCANNNNNTGG 1 cut(s) 370
PinAI ACCGGT 1 cut(s) 153
PkrI GCNGC 5 cut(s) 641, 831, 834, 837, 997
PshBI ATTAAT 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 181, 739
PspPI GGNCC 2 cut(s) 276, 866
PstI CTGCAG 1 cut(s) 641
PsuI RGATCY 2 cut(s) 74, 978
SaqAI TTAA 4 cut(s) 24, 267, 680, 714
SatI GCNGC 5 cut(s) 640, 830, 833, 836, 996
Sau3AI GATC 8 cut(s) 74, 238, 292, 691, 754, 913, 919, 978
Sau96I GGNCC 2 cut(s) 276, 866
ScrFI CCNGG 1 cut(s) 135
SfaNI GCATC 4 cut(s) 94, 323, 344, 808
SfcI CTRYAG 1 cut(s) 637
SmlI CTYRAG 1 cut(s) 679
SmoI CTYRAG 1 cut(s) 679
SpeI ACTAGT 1 cut(s) 229
Sse9I AATT 8 cut(s) 14, 111, 251, 411, 449, 527, 665, 938
SsiI CCGC 3 cut(s) 194, 559, 815
SspMI CTAG 3 cut(s) 48, 230, 242
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 3 cut(s) 164, 629, 737
TaiI ACGT 1 cut(s) 580
TaqI TCGA 2 cut(s) 256, 519
TaqII GACCGA 1 cut(s) 554
TasI AATT 8 cut(s) 14, 111, 251, 411, 449, 527, 665, 938
TfiI GAWTC 1 cut(s) 860
Tru1I TTAA 4 cut(s) 24, 267, 680, 714
Tru9I TTAA 4 cut(s) 24, 267, 680, 714
TscAI CASTG 1 cut(s) 889
TseI GCWGC 5 cut(s) 639, 829, 832, 835, 995
TspDTI ATGAA 3 cut(s) 30, 89, 737
TspGWI ACGGA 1 cut(s) 940
TspRI CASTG 1 cut(s) 889
Van91I CCANNNNNTGG 1 cut(s) 370
Vha464I CTTAAG 1 cut(s) 679
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 4 cut(s) 14, 251, 665, 938
XmiI GTMKAC 1 cut(s) 166
XspI CTAG 3 cut(s) 48, 230, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.