Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007986

Physical Location & Seq

Genomic Coordinates Reverse (-)

29899481 .. 29902426

Gene Structure 2946 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1222200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1719 bp

ATGAGTAAACAGGAAGTGATGAAGCTTCAGACATGTGTTCTCAAAGTTAACATTGACTGTGATGGATGCAAGCACAAGATAAAGAAATTGCTGCAAAAAATTGATGGTGTGTACACAACAAGTATTGATGCAGAACACAGCAAGGTGACTGTGACAGGGAATGTTGATCCACACACAATCATCAAGAAACTTGAAAAGAAGGGGAAACATGCAGAACTCTGGGGAGGGTCAAAGGGTTCAAATTTCAATCAAGCCCAGCTCAACAACCAGTTCAAGAACATGGCAATGCCGGTACAGAATGCGAAAGGCGGGGGACACCAGCAACAACCACCAAAGGGCGGAGGAGGCCACCAGCAACAACCGCAAAAGGGCGGAGGAGGCCACCAGCAACAGCACCAACAAATGAAAGGAGGCAATGGTAATGTTATGAAGGTGCCTCAGAAGAACCAGAAGTCTGTGAAGTTCAATCTGCCGGAGGCGGATGACTTTGACGGTAGCGATTTCGATGAGTTTGATGATGAGTTTGATGATGAGTTCGATGATGATGAGTTTGATGATGATGACTATGATGATGAGGAAGAGCTTGATCATGGACATCATCAGATGGCTAAGAACAAGGTGATGGGTAATATTGTAAATGGGCCACAAGGAGGAGGGCCATATGGGAAAATGATGCCAATGATGCCAATGATGGGTGCTAATCATGGGCATGGCCCACATGGGCCTGCTGGGATGATGAATATGCATGTGATGAACGACAAAAAGCTAGGTGGTGGGGGCGGCGGTGGGGGAGGGTCTGCCAAGAAAGGTGGGGTAATTGATTTCCCTGTGCAAATGAAAGGTATGGGTGGAAATAATGAAAAGAAGAATGGCAAGGACGGAAATGGCGGAAATAAAGGCGGTGGAAATAGCAAGGGTGGGGATAAGAAGCAAGGGGGTAAAAATAAGAAAGAGGGCAAAGATGGCAAAAAAGGAGGTGGATTGCTAGGGTGGCTCAGCCGGAGTACAAGTATCGGACGCGGCGGTTCCAAAGACGAGAAAGGTAACGGCAAGGGCAATGGAAGCAAAAAGGGCGGGGGTAAAGATGGAAAAAATGATTTTCATGAGATTGATTTCAAAGGGAAAGATACCAAAGGAGGGAAAGGAGGCAAAGGTGGAAAGGAAAGCAAGGAGGGCAAAAATGGAAAAGGAGCCAAAGAGGGCAAAGGAGGGAAAGGCGCCAAAGGTGGTGATGATGGTGAGGATATGCGGCAAATGGGTTACATGGGCCAAAACGGACAAATGGGTCAAAAGGGTCAAATGGGTCAAATGGGTCATATGGGAAATATGGGTCAGATGGGGCAGAGACCTCAAATGGGTCAGATGGGTCAGATGGGTCAAATGGGTAGCTATCCAATGGGGCAGATGGGTAATTTTCCTGCAGTACAAGGACTGCCTGCACAGGCAATGATGAACGGCGGAGGAGGATACTATCAAGGCGGTATGGGTCCCGGAAACCCGTATAATCAGCAGCAGCAACAATACTTGGCCATGATGATGAATCAACAGAGAGCAGCAGCCAACGGTGGAAATGATATGTACCATCAGATGATGTACGCCCGCCAGCAGCCGCCGCCGATGAGCTACATGCCCCAACAGCCAATGCCGTCAGGACCTGCATCTGATCCTTATGCCAACTACTTCAGTGATGAGAACCCTAATGGTTGTAGTATCATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0009506 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0030054 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055044 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

573

Amino Acids

60.7

Weight (kDa)

9.04

Isoelectric Point (pI)

34.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	14 - 70	4.2e-15	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05220 AT3G05220
fragaria_vesca	FvH4_6g05480 FvH4_6g05480 FvH4_6g05480 FvH4_6g05480
malus_domestica	MD04G1208200.v1.1 MD12G1222200.v1.1
prunus_persica	Prupe.6G329100_v2.0.a1 Prupe.6G329100_v2.0.a1
pyrus_communis	pycom04g18420 pycom12g20840
rosa_chinensis	RchiOBHm_Chr3g0454261
rosa_laevigata	RLG00000025471
rosa_multiflora	Rmu_sc0005231.1_g000005
rosa_roxburghii	Rroxscaffold_165G00437040 Rroxscaffold_6G00425300
rosa_rugosa	Rorug02G0653600
rosa_samantha	Rh3AG057200 Rh3BG058700 Rh3CG057900 Rh3DG059200
rosa_wichuraiana	Rw0G001730 Rw3G004400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1284
Acc36I	ACCTGC	1 cut(s)	1663
AccB1I	GGYRCC	2 cut(s)	433, 1217
AccII	CGCG	1 cut(s)	1020
AclWI	GGATC	2 cut(s)	161, 1658
AcoI	YGGCCR	1 cut(s)	1527
AcsI	RAATTY	1 cut(s)	241
AcuI	CTGAAG	2 cut(s)	11, 1666
AcyI	GRCGYC	1 cut(s)	1218
AfaI	GTAC	6 cut(s)	113, 294, 1006, 1425, 1580, 1595
AfiI	CCNNNNNNNGG	7 cut(s)	335, 338, 368, 650, 692, 1137, 1355
AflIII	ACRYGT	1 cut(s)	32
AgsI	TTSAA	6 cut(s)	194, 240, 247, 274, 466, 1117
AluBI	AGCT	6 cut(s)	25, 259, 583, 766, 1389, 1623
AluI	AGCT	6 cut(s)	25, 259, 583, 766, 1389, 1623
Alw26I	GTCTC	1 cut(s)	1339
AlwI	GGATC	2 cut(s)	161, 1658
AlwNI	CAGNNNCTG	1 cut(s)	1655
AoxI	GGCC	8 cut(s)	346, 379, 641, 656, 712, 722, 1267, 1527
ApeKI	GCWGC	6 cut(s)	91, 1510, 1513, 1553, 1556, 1606
ApoI	RAATTY	1 cut(s)	241
AspLEI	GCGC	1 cut(s)	1220
AspS9I	GGNCC	7 cut(s)	641, 656, 713, 722, 1267, 1487, 1652
AsuC2I	CCSGG	1 cut(s)	1491
AsuHPI	GGTGA	4 cut(s)	157, 631, 1241, 1250
AvaII	GGWCC	2 cut(s)	1487, 1652
BalI	TGGCCA	1 cut(s)	1529
BanI	GGYRCC	2 cut(s)	433, 1217
BbvI	GCAGC	6 cut(s)	78, 1522, 1525, 1565, 1568, 1618
BceAI	ACGGC	3 cut(s)	1063, 1471, 1630
BciVI	GTATCC	1 cut(s)	1460
BclI	TGATCA	1 cut(s)	586
BcnI	CCSGG	1 cut(s)	1491
BcoDI	GTCTC	1 cut(s)	1339
BfaI	CTAG	2 cut(s)	767, 986
BfmI	CTRYAG	1 cut(s)	1419
BfoI	RGCGCY	1 cut(s)	1221
BfuAI	ACCTGC	1 cut(s)	1663
BfuI	GTATCC	1 cut(s)	1460
BlpI	GCTNAGC	1 cut(s)	995
Bme1390I	CCNGG	1 cut(s)	1491
Bme18I	GGWCC	2 cut(s)	1487, 1652
BmgT120I	GGNCC	7 cut(s)	641, 656, 713, 722, 1267, 1487, 1652
BmiI	GGNNCC	6 cut(s)	435, 1027, 1192, 1219, 1488, 1489
BmrFI	CCNGG	1 cut(s)	1491
BmsI	GCATC	5 cut(s)	56, 118, 663, 672, 1667
Bpu1102I	GCTNAGC	1 cut(s)	995
BpuMI	CCSGG	1 cut(s)	1491
BsaHI	GRCGYC	1 cut(s)	1218
BsaI	GGTCTC	1 cut(s)	1339
BsaXI	ACNNNNNCTCC	2 cut(s)	994, 1024
Bsc4I	CCNNNNNNNGG	7 cut(s)	335, 338, 368, 650, 692, 1137, 1355
Bse118I	RCCGGY	1 cut(s)	289
Bse1I	ACTGG	1 cut(s)	268
Bse3DI	GCAATG	4 cut(s)	291, 421, 1063, 1452
BseGI	GGATG	3 cut(s)	71, 487, 738
BseLI	CCNNNNNNNGG	7 cut(s)	335, 338, 368, 650, 692, 1137, 1355
BseMI	GCAATG	4 cut(s)	291, 421, 1063, 1452
BseMII	CTCAG	2 cut(s)	452, 1009
BseNI	ACTGG	1 cut(s)	268
BseRI	GAGGAG	4 cut(s)	357, 390, 666, 1476
BseXI	GCAGC	6 cut(s)	78, 1522, 1525, 1565, 1568, 1618
BseYI	CCCAGC	2 cut(s)	255, 728
BsgI	GTGCAG	1 cut(s)	1422
Bsh1236I	CGCG	1 cut(s)	1020
BshFI	GGCC	8 cut(s)	348, 381, 643, 658, 714, 724, 1269, 1529
BshNI	GGYRCC	2 cut(s)	433, 1217
BsiSI	CCGG	4 cut(s)	290, 473, 1000, 1491
BslFI	GGGAC	2 cut(s)	327, 1473
BslI	CCNNNNNNNGG	7 cut(s)	335, 338, 368, 650, 692, 1137, 1355
BsmAI	GTCTC	1 cut(s)	1339
BsmFI	GGGAC	2 cut(s)	327, 1473
BsmI	GAATGC	1 cut(s)	304
BsnI	GGCC	8 cut(s)	348, 381, 643, 658, 714, 724, 1269, 1529
Bso31I	GGTCTC	1 cut(s)	1339
Bsp1407I	TGTACA	1 cut(s)	111
Bsp143I	GATC	3 cut(s)	166, 586, 1663
Bsp1720I	GCTNAGC	1 cut(s)	995
BspANI	GGCC	8 cut(s)	348, 381, 643, 658, 714, 724, 1269, 1529
BspCNI	CTCAG	2 cut(s)	451, 1008
BspFNI	CGCG	1 cut(s)	1020
BspHI	TCATGA	1 cut(s)	1102
BspLI	GGNNCC	6 cut(s)	435, 1027, 1192, 1219, 1488, 1489
BspMAI	CTGCAG	1 cut(s)	1423
BspMI	ACCTGC	1 cut(s)	1663
BspPI	GGATC	2 cut(s)	161, 1658
BspQI	GCTCTTC	1 cut(s)	573
BspT107I	GGYRCC	2 cut(s)	433, 1217
BspTNI	GGTCTC	1 cut(s)	1339
BsrDI	GCAATG	4 cut(s)	291, 421, 1063, 1452
BsrFI	RCCGGY	1 cut(s)	289
BsrGI	TGTACA	1 cut(s)	111
BsrI	ACTGG	1 cut(s)	268
BssAI	RCCGGY	1 cut(s)	289
BssMI	GATC	3 cut(s)	166, 586, 1663
BssNI	GRCGYC	1 cut(s)	1218
Bst4CI	ACNGT	4 cut(s)	59, 151, 494, 1565
Bst6I	CTCTTC	1 cut(s)	573
BstACI	GRCGYC	1 cut(s)	1218
BstAUI	TGTACA	1 cut(s)	111
BstC8I	GCNNGC	5 cut(s)	71, 726, 1437, 1600, 1604
BstDEI	CTNAG	3 cut(s)	438, 609, 995
BstF5I	GGATG	3 cut(s)	71, 487, 738
BstFNI	CGCG	1 cut(s)	1020
BstH2I	RGCGCY	1 cut(s)	1221
BstHHI	GCGC	1 cut(s)	1220
BstKTI	GATC	3 cut(s)	169, 589, 1666
BstMAI	GTCTC	1 cut(s)	1339
BstMBI	GATC	3 cut(s)	166, 586, 1663
BstNSI	RCATGY	4 cut(s)	36, 212, 749, 1630
BstSCI	CCNGG	1 cut(s)	1489
BstSFI	CTRYAG	1 cut(s)	1419
BstUI	CGCG	1 cut(s)	1020
BstV1I	GCAGC	6 cut(s)	78, 1522, 1525, 1565, 1568, 1618
BsuI	GTATCC	1 cut(s)	1460
BsuRI	GGCC	8 cut(s)	348, 381, 643, 658, 714, 724, 1269, 1529
BtsCI	GGATG	3 cut(s)	71, 487, 738
BtsIMutI	CAGTG	1 cut(s)	1690
BveI	ACCTGC	1 cut(s)	1663
Cac8I	GCNNGC	5 cut(s)	71, 726, 1437, 1600, 1604
CaiI	CAGNNNCTG	1 cut(s)	1655
CciI	TCATGA	1 cut(s)	1102
CfoI	GCGC	1 cut(s)	1220
Cfr10I	RCCGGY	1 cut(s)	289
Cfr13I	GGNCC	7 cut(s)	641, 656, 713, 722, 1267, 1487, 1652
CseI	GACGC	1 cut(s)	1026
Csp6I	GTAC	6 cut(s)	112, 293, 1005, 1424, 1579, 1594
CspCI	CAANNNNNGTGG	2 cut(s)	790, 825
CviQI	GTAC	6 cut(s)	112, 293, 1005, 1424, 1579, 1594
DdeI	CTNAG	3 cut(s)	438, 609, 995
DinI	GGCGCC	1 cut(s)	1219
DpnI	GATC	3 cut(s)	168, 588, 1665
DpnII	GATC	3 cut(s)	166, 586, 1663
DrdI	GACNNNNNNGTC	1 cut(s)	1284
DseDI	GACNNNNNNGTC	1 cut(s)	1284
EaeI	YGGCCR	1 cut(s)	1527
Eam1104I	CTCTTC	1 cut(s)	573
EarI	CTCTTC	1 cut(s)	573
EciI	GGCGGA	5 cut(s)	354, 387, 494, 903, 1473
Eco31I	GGTCTC	1 cut(s)	1339
Eco47I	GGWCC	2 cut(s)	1487, 1652
Eco57I	CTGAAG	2 cut(s)	11, 1666
EcoO109I	RGGNCCY	2 cut(s)	1487, 1652
EcoT22I	ATGCAT	1 cut(s)	747
EgeI	GGCGCC	1 cut(s)	1219
EheI	GGCGCC	1 cut(s)	1219
FaqI	GGGAC	2 cut(s)	327, 1473
FauI	CCCGC	3 cut(s)	302, 1067, 1607
FauNDI	CATATG	2 cut(s)	661, 1317
FbaI	TGATCA	1 cut(s)	586
FokI	GGATG	3 cut(s)	78, 494, 745
FspBI	CTAG	2 cut(s)	767, 986
GlaI	GCGC	1 cut(s)	1219
GsaI	CCCAGC	2 cut(s)	259, 732
HaeII	RGCGCY	1 cut(s)	1221
HaeIII	GGCC	8 cut(s)	348, 381, 643, 658, 714, 724, 1269, 1529
HapII	CCGG	4 cut(s)	290, 473, 1000, 1491
HgaI	GACGC	1 cut(s)	1026
HhaI	GCGC	1 cut(s)	1220
Hin1I	GRCGYC	1 cut(s)	1218
Hin6I	GCGC	1 cut(s)	1218
HinP1I	GCGC	1 cut(s)	1218
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	1 cut(s)	1540
HpaI	GTTAAC	1 cut(s)	49
HpaII	CCGG	4 cut(s)	290, 473, 1000, 1491
HphI	GGTGA	4 cut(s)	157, 631, 1241, 1250
Hpy166II	GTNNAC	4 cut(s)	8, 49, 112, 114
Hpy188I	TCNGA	9 cut(s)	30, 441, 603, 1016, 1335, 1362, 1371, 1587, 1663
Hpy188III	TCNNGA	4 cut(s)	184, 274, 1103, 1650
Hpy8I	GTNNAC	4 cut(s)	8, 49, 112, 114
HpyAV	CCTTC	2 cut(s)	193, 424
HpyCH4III	ACNGT	4 cut(s)	59, 151, 494, 1565
HpyCH4V	TGCA	9 cut(s)	69, 94, 131, 212, 745, 832, 1421, 1439, 1658
HpyF3I	CTNAG	3 cut(s)	438, 609, 995
Hsp92I	GRCGYC	1 cut(s)	1218
HspAI	GCGC	1 cut(s)	1218
KasI	GGCGCC	1 cut(s)	1217
KflI	GGGWCCC	1 cut(s)	1487
Ksp22I	TGATCA	1 cut(s)	586
KspAI	GTTAAC	1 cut(s)	49
Kzo9I	GATC	3 cut(s)	166, 586, 1663
LguI	GCTCTTC	1 cut(s)	573
LmnI	GCTCC	1 cut(s)	1190
Lsp1109I	GCAGC	6 cut(s)	78, 1522, 1525, 1565, 1568, 1618
LweI	GCATC	5 cut(s)	56, 118, 663, 672, 1667
MaeI	CTAG	2 cut(s)	767, 986
MaeIII	GTNAC	4 cut(s)	145, 151, 1043, 1259
MalI	GATC	3 cut(s)	168, 588, 1665
MboI	GATC	3 cut(s)	166, 586, 1663
MboII	GAAGA	3 cut(s)	454, 590, 877
MlsI	TGGCCA	1 cut(s)	1529
MluCI	AATT	5 cut(s)	86, 99, 241, 816, 1411
MluNI	TGGCCA	1 cut(s)	1529
Mly113I	GGCGCC	1 cut(s)	1218
Mox20I	TGGCCA	1 cut(s)	1529
Mph1103I	ATGCAT	1 cut(s)	747
MscI	TGGCCA	1 cut(s)	1529
MseI	TTAA	1 cut(s)	48
MslI	CAYNNNNRTG	3 cut(s)	284, 708, 1535
Msp20I	TGGCCA	1 cut(s)	1529
MspI	CCGG	4 cut(s)	290, 473, 1000, 1491
MspR9I	CCNGG	1 cut(s)	1491
Mva1269I	GAATGC	1 cut(s)	304
MvnI	CGCG	1 cut(s)	1020
NarI	GGCGCC	1 cut(s)	1218
NciI	CCSGG	1 cut(s)	1491
NdeI	CATATG	2 cut(s)	661, 1317
NdeII	GATC	3 cut(s)	166, 586, 1663
NlaIV	GGNNCC	6 cut(s)	435, 1027, 1192, 1219, 1488, 1489
NmuCI	GTSAC	2 cut(s)	145, 151
NsiI	ATGCAT	1 cut(s)	747
NspI	RCATGY	4 cut(s)	36, 212, 749, 1630
PagI	TCATGA	1 cut(s)	1102
PciI	ACATGT	1 cut(s)	32
PciSI	GCTCTTC	1 cut(s)	573
PctI	GAATGC	1 cut(s)	304
PfeI	GAWTC	1 cut(s)	1540
PfoI	TCCNGGA	1 cut(s)	1489
PluTI	GGCGCC	1 cut(s)	1221
PpuMI	RGGWCCY	2 cut(s)	1487, 1652
PscI	ACATGT	1 cut(s)	32
Psp5II	RGGWCCY	2 cut(s)	1487, 1652
PspFI	CCCAGC	2 cut(s)	255, 728
PspN4I	GGNNCC	6 cut(s)	435, 1027, 1192, 1219, 1488, 1489
PspPI	GGNCC	7 cut(s)	641, 656, 713, 722, 1267, 1487, 1652
PspPPI	RGGWCCY	2 cut(s)	1487, 1652
PstI	CTGCAG	1 cut(s)	1423
PstNI	CAGNNNCTG	1 cut(s)	1655
RsaI	GTAC	6 cut(s)	113, 294, 1006, 1425, 1580, 1595
RsaNI	GTAC	6 cut(s)	112, 293, 1005, 1424, 1579, 1594
RseI	CAYNNNNRTG	3 cut(s)	284, 708, 1535
SapI	GCTCTTC	1 cut(s)	573
SaqAI	TTAA	1 cut(s)	48
Sau3AI	GATC	3 cut(s)	166, 586, 1663
Sau96I	GGNCC	7 cut(s)	641, 656, 713, 722, 1267, 1487, 1652
ScrFI	CCNGG	1 cut(s)	1491
SfaNI	GCATC	5 cut(s)	56, 118, 663, 672, 1667
SfcI	CTRYAG	1 cut(s)	1419
SfoI	GGCGCC	1 cut(s)	1219
SinI	GGWCC	2 cut(s)	1487, 1652
SmiMI	CAYNNNNRTG	3 cut(s)	284, 708, 1535
Sse9I	AATT	5 cut(s)	86, 99, 241, 816, 1411
SspDI	GGCGCC	1 cut(s)	1217
SspI	AATATT	1 cut(s)	631
SspMI	CTAG	2 cut(s)	767, 986
StyD4I	CCNGG	1 cut(s)	1489
TaaI	ACNGT	4 cut(s)	59, 151, 494, 1565
TaqI	TCGA	2 cut(s)	504, 537
TasI	AATT	5 cut(s)	86, 99, 241, 816, 1411
TatI	WGTACW	3 cut(s)	111, 1004, 1423
TauI	GCSGC	5 cut(s)	783, 1023, 1252, 1612, 1615
TfiI	GAWTC	1 cut(s)	1540
Tru1I	TTAA	1 cut(s)	48
Tru9I	TTAA	1 cut(s)	48
TscAI	CASTG	1 cut(s)	1690
TseFI	GTSAC	2 cut(s)	145, 151
TseI	GCWGC	6 cut(s)	91, 1510, 1513, 1553, 1556, 1606
Tsp45I	GTSAC	2 cut(s)	145, 151
TspGWI	ACGGA	2 cut(s)	894, 1290
TspRI	CASTG	1 cut(s)	1690
VpaK11BI	GGWCC	2 cut(s)	1487, 1652
XapI	RAATTY	1 cut(s)	241
XceI	RCATGY	4 cut(s)	36, 212, 749, 1630
XspI	CTAG	2 cut(s)	767, 986
Zsp2I	ATGCAT	1 cut(s)	747

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.