MD12G1241800.v1.1

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009806
Physical Location & Seq
Forward (+)
31346637 .. 31349429
2793 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1241800.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGGTCGTGTGATACACGAAGAGCAGGATCAGAAATACAGCTTGATAAACTTTGAGGAGACAGAACTGCGTCTGGGGTTGCCTGGTGGAATATTAGGCACTGCTGGAAAGGATGCTGAAGCTTCCAAGAACACTGGAAAGAGAGGGTTTGCTGAAACTGTTGATTTGAAGCTCAATATTTCATCTGAAGATCAATCGGCTGGTGATGAAGATCAAGCGGTGGAGATGAAGGAGAAGAATGTTGCTCCTGTTCCTAGGTCAAATGATCCAACTAAGCCTCCTGCTGCCAAGGCTCAAGTTGTAGGTTGGCCCCCTGTCCGATCATTCCGAAAGAACATCCTGACCGTCCAAAAGAATAGCTCTGAGGAGGGTGAAAATACCAACAGCATCAGTGCAGCATTTGTTAAAGTCAGCATGGATGGCGCACCCTACTTGCGTAAAGTGGACCTGAAGTTGTACAAAAGCTACCAAGAGCTTTCTACTGCCTTAAGCAAAATGTTTAGCTCCTTCACCATTGGTAACTGTGGGTCACAAGGAATGAAGGATTTCATGAACGAGAGCAAACTAATAGACCTTCTGAGTGGTTCTGAGTATGTGCCTAGTTATGAGGACAAGGATGGAGATTGGATGCTTGTTGGAGATGTGCCATGGGAAATGTTTGTCAATTCATGCAAACGCCTGCGAATAATGAAGGGATCTGAGGCTATCGGACTTGGTTACACCAAGAGCGGTGGAGAAGTACAAGAACAGAAGTTGAAGAAGTTGCTAGATTGCCGTGGGTTGATATTTCTGCCATGGACACAATCAAATGAAAGCAGACCGGCTCTACACAGATGGAGAGCACGAAGTTTGGATGTCTGTGACTCTGTATTTTTGTGCCTTTTTCTTTTTGGTCGTCTAATGTTGTTAATTAAAGTTACATGGAAGAATATATTCGGAGATTTGTTAGGGACAAGAAGCAATAATATATATTATAGAAGATGTTCATACTTCATAGTGGCTCATGTGCAACTTTCTTTATTGTCCGTAGCCCTACTGTTTAATAATTATATAATATATATTGCCAGTGTTACTTGTCAATAG

Protein Analysis

361

Amino Acids

40.32

Weight (kDa)

8.33

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 20 - 239 1.5e-88 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 729
AciI CCGC 2 cut(s) 218, 729
AclWI GGATC 3 cut(s) 36, 260, 703
AcuI CTGAAG 3 cut(s) 138, 207, 470
AfaI GTAC 2 cut(s) 458, 741
AflII CTTAAG 1 cut(s) 487
AgsI TTSAA 2 cut(s) 169, 757
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 7 cut(s) 42, 122, 172, 360, 465, 475, 504
AluI AGCT 7 cut(s) 42, 122, 172, 360, 465, 475, 504
Alw21I GWGCWC 1 cut(s) 844
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 3 cut(s) 36, 260, 703
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 2 cut(s) 284, 395
Asp700I GAANNNNTTC 3 cut(s) 545, 932, 982
AspA2I CCTAGG 1 cut(s) 254
AspLEI GCGC 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 309, 445
AsuHPI GGTGA 3 cut(s) 215, 383, 502
AvaII GGWCC 1 cut(s) 445
AvrII CCTAGG 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 844
BbvI GCAGC 2 cut(s) 271, 407
BccI CCATC 3 cut(s) 413, 611, 828
BceAI ACGGC 1 cut(s) 759
BciT130I CCWGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 3 cut(s) 255, 600, 767
BfrI CTTAAG 1 cut(s) 487
BisI GCNGC 2 cut(s) 285, 396
BlnI CCTAGG 1 cut(s) 254
BlsI GCNGC 2 cut(s) 286, 397
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 1 cut(s) 445
BmgT120I GGNCC 2 cut(s) 309, 445
BmiI GGNNCC 1 cut(s) 311
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 3 cut(s) 103, 396, 618
BpuEI CTTGAG 1 cut(s) 279
BsaBI GATNNNNATC 1 cut(s) 210
BsaJI CCNNGG 5 cut(s) 254, 288, 647, 775, 794
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bse118I RCCGGY 1 cut(s) 820
Bse1I ACTGG 2 cut(s) 139, 1065
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 5 cut(s) 254, 288, 647, 775, 794
BseGI GGATG 6 cut(s) 118, 336, 424, 622, 633, 859
BseJI GATNNNNATC 1 cut(s) 210
BseMII CTCAG 4 cut(s) 354, 569, 579, 690
BseNI ACTGG 2 cut(s) 139, 1065
BseRI GAGGAG 2 cut(s) 71, 380
BseXI GCAGC 2 cut(s) 271, 407
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 844
BsiSI CCGG 1 cut(s) 821
BslFI GGGAC 1 cut(s) 964
BsmAI GTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 964
BsnI GGCC 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 844
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 6 cut(s) 28, 190, 211, 265, 320, 695
Bsp19I CCATGG 2 cut(s) 647, 794
BspACI CCGC 2 cut(s) 218, 729
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 4 cut(s) 355, 570, 580, 691
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 1 cut(s) 311
BspPI GGATC 3 cut(s) 36, 260, 703
BspQI GCTCTTC 1 cut(s) 15
BspTI CTTAAG 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 820
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 2 cut(s) 139, 1065
BssAI RCCGGY 1 cut(s) 820
BssECI CCNNGG 5 cut(s) 254, 288, 647, 775, 794
BssMI GATC 6 cut(s) 28, 190, 211, 265, 320, 695
BssT1I CCWWGG 4 cut(s) 254, 288, 647, 794
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 4 cut(s) 160, 346, 524, 1038
Bst6I CTCTTC 1 cut(s) 15
BstAFI CTTAAG 1 cut(s) 487
BstAUI TGTACA 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 680
BstDEI CTNAG 5 cut(s) 273, 363, 578, 588, 699
BstDSI CCRYGG 3 cut(s) 647, 775, 794
BstF5I GGATG 6 cut(s) 118, 336, 424, 622, 633, 859
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 6 cut(s) 31, 193, 214, 268, 323, 698
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 6 cut(s) 28, 190, 211, 265, 320, 695
BstMWI GCNNNNNNNGC 2 cut(s) 290, 420
BstNI CCWGG 1 cut(s) 84
BstSCI CCNGG 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 271, 407
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 3 cut(s) 647, 775, 794
BtsCI GGATG 6 cut(s) 118, 336, 424, 622, 633, 859
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 4 cut(s) 99, 132, 397, 1072
Cac8I GCNNGC 1 cut(s) 680
CciI TCATGA 1 cut(s) 549
CfoI GCGC 1 cut(s) 425
Cfr10I RCCGGY 1 cut(s) 820
Cfr13I GGNCC 2 cut(s) 309, 445
CseI GACGC 1 cut(s) 59
Csp6I GTAC 2 cut(s) 457, 740
CspCI CAANNNNNGTGG 2 cut(s) 712, 747
CviAII CATG 7 cut(s) 415, 550, 648, 669, 795, 921, 1004
CviQI GTAC 2 cut(s) 457, 740
DdeI CTNAG 5 cut(s) 273, 363, 578, 588, 699
DpnI GATC 6 cut(s) 30, 192, 213, 267, 322, 697
DpnII GATC 6 cut(s) 28, 190, 211, 265, 320, 695
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
Eco130I CCWWGG 4 cut(s) 254, 288, 647, 794
Eco47I GGWCC 1 cut(s) 445
Eco57I CTGAAG 3 cut(s) 138, 207, 470
EcoRII CCWGG 1 cut(s) 82
EcoT14I CCWWGG 4 cut(s) 254, 288, 647, 794
ErhI CCWWGG 4 cut(s) 254, 288, 647, 794
FaeI CATG 7 cut(s) 418, 553, 651, 672, 798, 924, 1007
FaqI GGGAC 1 cut(s) 964
FatI CATG 7 cut(s) 414, 549, 647, 668, 794, 920, 1003
Fnu4HI GCNGC 2 cut(s) 285, 396
FokI GGATG 6 cut(s) 125, 323, 431, 629, 640, 866
Fsp4HI GCNGC 2 cut(s) 285, 396
FspBI CTAG 3 cut(s) 255, 600, 767
GlaI GCGC 1 cut(s) 424
GluI GCNGC 2 cut(s) 285, 396
HaeIII GGCC 1 cut(s) 310
HapII CCGG 1 cut(s) 821
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 425
Hin1II CATG 7 cut(s) 418, 553, 651, 672, 798, 924, 1007
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 1 cut(s) 863
HpaII CCGG 1 cut(s) 821
HphI GGTGA 3 cut(s) 215, 383, 502
Hpy166II GTNNAC 1 cut(s) 445
Hpy188III TCNNGA 2 cut(s) 340, 550
Hpy8I GTNNAC 1 cut(s) 445
HpyAV CCTTC 5 cut(s) 223, 517, 535, 584, 685
HpyCH4III ACNGT 4 cut(s) 160, 346, 524, 1038
HpyCH4V TGCA 3 cut(s) 395, 672, 1009
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 420
HpyF3I CTNAG 5 cut(s) 273, 363, 578, 588, 699
Hsp92II CATG 7 cut(s) 418, 553, 651, 672, 798, 924, 1007
HspAI GCGC 1 cut(s) 423
Kzo9I GATC 6 cut(s) 28, 190, 211, 265, 320, 695
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 2 cut(s) 250, 509
Lsp1109I GCAGC 2 cut(s) 271, 407
LweI GCATC 3 cut(s) 103, 396, 618
MaeI CTAG 3 cut(s) 255, 600, 767
MaeIII GTNAC 6 cut(s) 518, 528, 716, 860, 916, 1069
MalI GATC 6 cut(s) 30, 192, 213, 267, 322, 697
MbiI CCGCTC 1 cut(s) 729
MboI GATC 6 cut(s) 28, 190, 211, 265, 320, 695
MboII GAAGA 7 cut(s) 32, 200, 221, 247, 769, 937, 990
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 1 cut(s) 844
MluCI AATT 3 cut(s) 664, 909, 1045
MlyI GAGTC 1 cut(s) 857
MmeI TCCRAC 2 cut(s) 293, 616
MnlI CCTC 7 cut(s) 49, 137, 288, 358, 361, 601, 694
MroXI GAANNNNTTC 3 cut(s) 545, 932, 982
MseI TTAA 5 cut(s) 405, 488, 908, 912, 1041
MspCI CTTAAG 1 cut(s) 487
MspI CCGG 1 cut(s) 821
MspR9I CCNGG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 2 cut(s) 290, 420
NcoI CCATGG 2 cut(s) 647, 794
NdeII GATC 6 cut(s) 28, 190, 211, 265, 320, 695
NlaIII CATG 7 cut(s) 418, 553, 651, 672, 798, 924, 1007
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 2 cut(s) 528, 860
PacI TTAATTAA 1 cut(s) 912
PagI TCATGA 1 cut(s) 549
PciSI GCTCTTC 1 cut(s) 15
PdmI GAANNNNTTC 3 cut(s) 545, 932, 982
PkrI GCNGC 2 cut(s) 286, 397
PleI GAGTC 1 cut(s) 857
PpsI GAGTC 1 cut(s) 857
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 2 cut(s) 309, 445
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 2 cut(s) 458, 741
RsaNI GTAC 2 cut(s) 457, 740
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 5 cut(s) 405, 488, 908, 912, 1041
SatI GCNGC 2 cut(s) 285, 396
Sau3AI GATC 6 cut(s) 28, 190, 211, 265, 320, 695
Sau96I GGNCC 2 cut(s) 309, 445
SchI GAGTC 1 cut(s) 857
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 1 cut(s) 844
SfaNI GCATC 3 cut(s) 103, 396, 618
SinI GGWCC 1 cut(s) 445
SmlI CTYRAG 2 cut(s) 294, 487
SmoI CTYRAG 2 cut(s) 294, 487
Sse9I AATT 3 cut(s) 664, 909, 1045
SsiI CCGC 2 cut(s) 218, 729
SspI AATATT 2 cut(s) 93, 178
SspMI CTAG 3 cut(s) 255, 600, 767
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 4 cut(s) 254, 288, 647, 794
TaaI ACNGT 4 cut(s) 160, 346, 524, 1038
TasI AATT 3 cut(s) 664, 909, 1045
TatI WGTACW 2 cut(s) 456, 739
Tru1I TTAA 5 cut(s) 405, 488, 908, 912, 1041
Tru9I TTAA 5 cut(s) 405, 488, 908, 912, 1041
TscAI CASTG 4 cut(s) 106, 139, 397, 1072
TseFI GTSAC 2 cut(s) 528, 860
TseI GCWGC 2 cut(s) 284, 395
Tsp45I GTSAC 2 cut(s) 528, 860
TspGWI ACGGA 1 cut(s) 1015
TspRI CASTG 4 cut(s) 106, 139, 397, 1072
Vha464I CTTAAG 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 445
XmaJI CCTAGG 1 cut(s) 254
XmnI GAANNNNTTC 3 cut(s) 545, 932, 982
XspI CTAG 3 cut(s) 255, 600, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.