Rorug02G0631000

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005181
Physical Location & Seq
Reverse (-)
74591308 .. 74593962
2655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0631000.1

Sequence Viewer

Length: 1506 bp
ATGTTTGGATTTTCCCCCATTTTATTATCTTTCTTGCTTGTACTACTACCAGCACTGGCCTATATTCTTCTCATCAAATTGTTTCGAAGTAGACATGCCAAGCTTGCTGATCATGCTGCTAAAAACTTGCCGCCGGGAAGCATGGGATGGCCGGTTTTCGGCGAAACTCTTCACTTCCTCAAGCCTCATTCTTCGGATTCTCCCGGAACCTTCCTGCAACAACACTGCTCTAGGTATGGGAAGGTTTTCAAGTCGCATCTATTTGGTTCGCCGACCATAGTTTCGTGCGACCTTGAGCTCAACATGTTCGTTCTTCAGAACGAAGAGAAGCTCTTCCAAGCCAGTTACCCAAAACCCGTCCATGGCATTCTCGGGAAGTACTCTTTGCTCACCGTCTCCGGTGACCTTCACCGGAAGCTAAGGAACATTGGCGTCAGCTTCATCGCCTCTTCCAAGTCCTCGCCTCATTTTCTTCACTGGATCGAGAAGCTCTCGATCTCGATGATGGACTCGTGGAGTGGCCGAAATCAAGTCTCCTTCTTTAAAGAAGCTAAAGCTTTTACACTTCATCTTATGGTGAAGCACCTCTTGAGTGTTAACCCCGAGGAGGCACTAGCTTCGAGAATATTAGAAGACTTCCAAACGTACATGAGAGGCTTCGTTTCTCTGCCGATAAATCTCCCCGGAACCGCTTATGCCAAGGCTGTTAAGGCTAGAGCGAGGCTTTCTTCGAGTGTCAAGGAGATTATGGAAGGAAGGCGAAAACGGAGAATCGGAGAGCTTGCAGAATTAGGAGACGGAGAAGTGGACTTTTTAGACGCGATACTGTCGAAACAAAGCCTAAACGACGATGAAATAGTAAGCATTGTGTTGGACATAATGCTGGGAGGGTACGAGACCACCTCAACGCTAATGGCCTTGATTGTCTACTTTCTTCATCATTCGCCAATTGCTTTTGCCAAATTAAAGGAAGAACACCAAGCTATAAGGGAAAACAAAGAGGATGAGAGTGCTCCCTTGGACTGGGAAGATTACAAGAAAATGGAGTACTCTTACAATGTTATATGTGAAGCCATGAGATGTGGCAATGTAGTTAAATTTGTGCACAGAAAAGCTCTTCAAGATGTGAAATTTAAAGAGCTGGTCATACCGTCCGGATGGAAGGTTCTTCCGATCTTCACCGCATCACATATGGATCCAGATCTTCACGATAATCCGGCACAATTTGATCCTTCAAGATGGACTAGTGATCATTACAAAGAAATGAGCAGAAAAGTGACACCGTTTGGTGGAGGAGCAAGGCTTTGTCCAGGAGCTGAGCTCGCTAAAGTAGTCATTTCCTTCTTCCTTCATCATCTAGTCCTTACATATAGGTGGAAAACAAAATCAGATGAGTGCCCTCTTGCTTACCCCTATGTCGAATTCCGAAGAGGTTTACTGATAGAGATTGAACCTGCTGCGGCCGGAAGTGACTTGATCTACAGGGAGATGAAATTGAGCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

501

Amino Acids

56.73

Weight (kDa)

8.65

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 44 - 467 4e-61 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1462
AccI GTMKAC 2 cut(s) 91, 927
AccII CGCG 1 cut(s) 821
AccIII TCCGGA 1 cut(s) 1154
AciI CCGC 4 cut(s) 131, 690, 1182, 1460
AclWI GGATC 4 cut(s) 488, 1190, 1203, 1223
AcoI YGGCCR 3 cut(s) 149, 520, 1461
AcsI RAATTY 3 cut(s) 1097, 1130, 1421
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 432
AfaI GTAC 5 cut(s) 42, 380, 647, 893, 1049
AfiI CCNNNNNNNGG 5 cut(s) 158, 362, 607, 1023, 1289
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 4 cut(s) 250, 1121, 1236, 1451
AhlI ACTAGT 1 cut(s) 1244
AjnI CCWGG 1 cut(s) 1309
AloI GAACNNNNNNTCC 2 cut(s) 1149, 1181
Alw21I GWGCWC 4 cut(s) 300, 1015, 1107, 1323
Alw26I GTCTC 4 cut(s) 400, 538, 789, 890
Alw44I GTGCAC 1 cut(s) 1103
AlwI GGATC 4 cut(s) 488, 1190, 1203, 1223
AlwNI CAGNNNCTG 1 cut(s) 1316
Ama87I CYCGRG 2 cut(s) 371, 602
Aor13HI TCCGGA 1 cut(s) 1154
AoxI GGCC 5 cut(s) 57, 149, 520, 915, 1461
ApaLI GTGCAC 1 cut(s) 1103
ApeKI GCWGC 2 cut(s) 116, 1457
ApoI RAATTY 3 cut(s) 1097, 1130, 1421
ArsI GACNNNNNNTTYG 2 cut(s) 265, 297
Asp700I GAANNNNTTC 3 cut(s) 168, 245, 332
AsuC2I CCSGG 3 cut(s) 135, 204, 684
AsuHPI GGTGA 5 cut(s) 382, 401, 413, 589, 1171
AsuII TTCGAA 1 cut(s) 85
AvaI CYCGRG 2 cut(s) 371, 602
BaeGI GKGCMC 2 cut(s) 1107, 1400
BamHI GGATCC 1 cut(s) 1195
BanII GRGCYC 3 cut(s) 300, 1323, 1502
BauI CACGAG 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 4 cut(s) 300, 1015, 1107, 1323
BbvI GCAGC 2 cut(s) 103, 1444
BccI CCATC 4 cut(s) 141, 499, 1152, 1233
BcgI CGANNNNNNTGC 2 cut(s) 600, 634
BciT130I CCWGG 1 cut(s) 1311
BclI TGATCA 2 cut(s) 109, 1249
BcnI CCSGG 3 cut(s) 135, 204, 684
BcoDI GTCTC 4 cut(s) 400, 538, 789, 890
BcuI ACTAGT 1 cut(s) 1244
BfaI CTAG 5 cut(s) 231, 614, 714, 1245, 1358
BfmI CTRYAG 1 cut(s) 1480
BfuAI ACCTGC 1 cut(s) 1462
BglII AGATCT 1 cut(s) 1201
BisI GCNGC 4 cut(s) 117, 131, 1458, 1461
BlpI GCTNAGC 1 cut(s) 1317
BlsI GCNGC 4 cut(s) 118, 132, 1459, 1462
BmcAI AGTACT 2 cut(s) 380, 1049
Bme1390I CCNGG 4 cut(s) 135, 204, 684, 1311
BmeT110I CYCGRG 2 cut(s) 371, 602
BmiI GGNNCC 3 cut(s) 208, 688, 1197
BmrFI CCNGG 4 cut(s) 135, 204, 684, 1311
BmrI ACTGGG 1 cut(s) 1033
BmsI GCATC 2 cut(s) 265, 1193
BmuI ACTGGG 1 cut(s) 1033
BpiI GAAGAC 1 cut(s) 639
BplI GAGNNNNNCTC 6 cut(s) 476, 508, 887, 919, 1305, 1337
Bpu10I CCTNAGC 1 cut(s) 419
Bpu1102I GCTNAGC 1 cut(s) 1317
Bpu14I TTCGAA 1 cut(s) 85
BpuEI CTTGAG 3 cut(s) 164, 314, 610
BpuMI CCSGG 3 cut(s) 135, 204, 684
BsaBI GATNNNNATC 1 cut(s) 1200
BsaHI GRCGYC 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 5 cut(s) 361, 603, 682, 699, 1017
BsaWI WCCGGW 3 cut(s) 398, 411, 1154
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 362, 607, 1023, 1289
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 4 cut(s) 60, 342, 482, 1028
Bse3DI GCAATG 1 cut(s) 1093
Bse8I GATNNNNATC 1 cut(s) 1200
BseAI TCCGGA 1 cut(s) 1154
BseBI CCWGG 1 cut(s) 1311
BseDI CCNNGG 5 cut(s) 361, 603, 682, 699, 1017
BseGI GGATG 3 cut(s) 152, 1009, 1163
BseJI GATNNNNATC 1 cut(s) 1200
BseLI CCNNNNNNNGG 5 cut(s) 158, 362, 607, 1023, 1289
BseMI GCAATG 1 cut(s) 1093
BseMII CTCAG 1 cut(s) 1308
BseNI ACTGG 4 cut(s) 60, 342, 482, 1028
BseRI GAGGAG 2 cut(s) 620, 1308
BseSI GKGCMC 2 cut(s) 1107, 1400
BseX3I CGGCCG 1 cut(s) 1461
BseXI GCAGC 2 cut(s) 103, 1444
BseYI CCCAGC 1 cut(s) 883
Bsh1236I CGCG 1 cut(s) 821
Bsh1285I CGRYCG 1 cut(s) 1464
BshFI GGCC 5 cut(s) 59, 151, 522, 917, 1463
BsiEI CGRYCG 1 cut(s) 1464
BsiHKAI GWGCWC 4 cut(s) 300, 1015, 1107, 1323
BsiHKCI CYCGRG 2 cut(s) 371, 602
BsiSI CCGG 9 cut(s) 134, 152, 204, 399, 412, 684, 1155, 1217, 1464
BslI CCNNNNNNNGG 5 cut(s) 158, 362, 607, 1023, 1289
BsmAI GTCTC 4 cut(s) 400, 538, 789, 890
BsmBI CGTCTC 2 cut(s) 400, 789
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 5 cut(s) 59, 151, 522, 917, 1463
Bso31I GGTCTC 1 cut(s) 890
BsoBI CYCGRG 2 cut(s) 371, 602
Bsp119I TTCGAA 1 cut(s) 85
Bsp1286I GDGCHC 6 cut(s) 300, 1015, 1107, 1323, 1400, 1502
Bsp13I TCCGGA 1 cut(s) 1154
Bsp143I GATC 9 cut(s) 109, 480, 495, 1173, 1195, 1201, 1228, 1249, 1476
Bsp1720I GCTNAGC 1 cut(s) 1317
Bsp19I CCATGG 1 cut(s) 361
BspACI CCGC 4 cut(s) 131, 690, 1182, 1460
BspANI GGCC 5 cut(s) 59, 151, 522, 917, 1463
BspCNI CTCAG 1 cut(s) 1309
BspEI TCCGGA 1 cut(s) 1154
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 3 cut(s) 208, 688, 1197
BspMI ACCTGC 1 cut(s) 1462
BspPI GGATC 4 cut(s) 488, 1190, 1203, 1223
BspQI GCTCTTC 2 cut(s) 338, 1122
BspT104I TTCGAA 1 cut(s) 85
BspTNI GGTCTC 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 1093
BsrFI RCCGGY 1 cut(s) 151
BsrI ACTGG 4 cut(s) 60, 342, 482, 1028
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 5 cut(s) 361, 603, 682, 699, 1017
BssMI GATC 9 cut(s) 109, 480, 495, 1173, 1195, 1201, 1228, 1249, 1476
BssNI GRCGYC 1 cut(s) 432
BssSI CACGAG 1 cut(s) 511
BssT1I CCWWGG 3 cut(s) 361, 699, 1017
Bst2BI CACGAG 1 cut(s) 511
Bst2UI CCWGG 1 cut(s) 1311
Bst4CI ACNGT 4 cut(s) 394, 828, 1152, 1284
Bst6I CTCTTC 6 cut(s) 174, 318, 338, 454, 1122, 1423
BstACI GRCGYC 1 cut(s) 432
BstBI TTCGAA 1 cut(s) 85
BstC8I GCNNGC 3 cut(s) 105, 783, 1323
BstDEI CTNAG 2 cut(s) 419, 1317
BstDSI CCRYGG 1 cut(s) 361
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 3 cut(s) 152, 1009, 1163
BstFNI CGCG 1 cut(s) 821
BstKTI GATC 9 cut(s) 112, 483, 498, 1176, 1198, 1204, 1231, 1252, 1479
BstMAI GTCTC 4 cut(s) 400, 538, 789, 890
BstMBI GATC 9 cut(s) 109, 480, 495, 1173, 1195, 1201, 1228, 1249, 1476
BstMCI CGRYCG 1 cut(s) 1464
BstMWI GCNNNNNNNGC 4 cut(s) 104, 113, 710, 1322
BstNI CCWGG 1 cut(s) 1311
BstNSI RCATGY 2 cut(s) 98, 307
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 4 cut(s) 133, 202, 682, 1309
BstSFI CTRYAG 1 cut(s) 1480
BstSLI GKGCMC 2 cut(s) 1107, 1400
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 2 cut(s) 103, 1444
BstV2I GAAGAC 1 cut(s) 639
BstX2I RGATCY 2 cut(s) 1195, 1201
BstYI RGATCY 2 cut(s) 1195, 1201
BstZI CGGCCG 1 cut(s) 1461
BsuRI GGCC 5 cut(s) 59, 151, 522, 917, 1463
BtgI CCRYGG 1 cut(s) 361
BtgZI GCGATG 1 cut(s) 427
BtsCI GGATG 3 cut(s) 152, 1009, 1163
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 3 cut(s) 53, 223, 475
BveI ACCTGC 1 cut(s) 1462
Cac8I GCNNGC 3 cut(s) 105, 783, 1323
CaiI CAGNNNCTG 1 cut(s) 1316
Cfr10I RCCGGY 1 cut(s) 151
CseI GACGC 2 cut(s) 421, 827
Csp6I GTAC 5 cut(s) 41, 379, 646, 892, 1048
CviAII CATG 7 cut(s) 95, 113, 142, 304, 362, 649, 1075
CviQI GTAC 5 cut(s) 41, 379, 646, 892, 1048
DdeI CTNAG 2 cut(s) 419, 1317
DpnI GATC 9 cut(s) 111, 482, 497, 1175, 1197, 1203, 1230, 1251, 1478
DpnII GATC 9 cut(s) 109, 480, 495, 1173, 1195, 1201, 1228, 1249, 1476
DraI TTTAAA 2 cut(s) 544, 1135
EaeI YGGCCR 3 cut(s) 149, 520, 1461
EagI CGGCCG 1 cut(s) 1461
Eam1104I CTCTTC 6 cut(s) 174, 318, 338, 454, 1122, 1423
EarI CTCTTC 6 cut(s) 174, 318, 338, 454, 1122, 1423
Ecl136II GAGCTC 2 cut(s) 298, 1321
EclXI CGGCCG 1 cut(s) 1461
Eco130I CCWWGG 3 cut(s) 361, 699, 1017
Eco24I GRGCYC 3 cut(s) 300, 1323, 1502
Eco31I GGTCTC 1 cut(s) 890
Eco52I CGGCCG 1 cut(s) 1461
Eco53kI GAGCTC 2 cut(s) 298, 1321
Eco57I CTGAAG 1 cut(s) 299
Eco88I CYCGRG 2 cut(s) 371, 602
Eco91I GGTNACC 1 cut(s) 401
EcoICRI GAGCTC 2 cut(s) 298, 1321
EcoO65I GGTNACC 1 cut(s) 401
EcoRI GAATTC 1 cut(s) 1421
EcoRII CCWGG 1 cut(s) 1309
EcoT14I CCWWGG 3 cut(s) 361, 699, 1017
EcoT38I GRGCYC 3 cut(s) 300, 1323, 1502
ErhI CCWWGG 3 cut(s) 361, 699, 1017
Esp3I CGTCTC 2 cut(s) 400, 789
FaeI CATG 7 cut(s) 98, 116, 145, 307, 365, 652, 1078
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FatI CATG 7 cut(s) 94, 112, 141, 303, 361, 648, 1074
FauNDI CATATG 1 cut(s) 1191
FbaI TGATCA 2 cut(s) 109, 1249
FblI GTMKAC 2 cut(s) 91, 927
Fnu4HI GCNGC 4 cut(s) 117, 131, 1458, 1461
FokI GGATG 3 cut(s) 159, 1016, 1170
FriOI GRGCYC 3 cut(s) 300, 1323, 1502
Fsp4HI GCNGC 4 cut(s) 117, 131, 1458, 1461
FspBI CTAG 5 cut(s) 231, 614, 714, 1245, 1358
GluI GCNGC 4 cut(s) 117, 131, 1458, 1461
GsaI CCCAGC 1 cut(s) 887
HaeIII GGCC 5 cut(s) 59, 151, 522, 917, 1463
HapII CCGG 9 cut(s) 134, 152, 204, 399, 412, 684, 1155, 1217, 1464
HgaI GACGC 2 cut(s) 421, 827
Hin1I GRCGYC 1 cut(s) 432
Hin1II CATG 7 cut(s) 98, 116, 145, 307, 365, 652, 1078
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 2 cut(s) 101, 555
HinfI GANTC 3 cut(s) 197, 509, 771
HpaI GTTAAC 1 cut(s) 598
HpaII CCGG 9 cut(s) 134, 152, 204, 399, 412, 684, 1155, 1217, 1464
HphI GGTGA 5 cut(s) 382, 401, 413, 589, 1171
Hpy166II GTNNAC 6 cut(s) 92, 598, 808, 928, 1105, 1436
Hpy188I TCNGA 6 cut(s) 196, 318, 776, 1173, 1390, 1427
Hpy8I GTNNAC 6 cut(s) 92, 598, 808, 928, 1105, 1436
Hpy99I CGWCG 1 cut(s) 851
HpyCH4III ACNGT 4 cut(s) 394, 828, 1152, 1284
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 3 cut(s) 217, 785, 1105
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 113, 710, 1322
HpyF3I CTNAG 2 cut(s) 419, 1317
HpySE526I ACGT 1 cut(s) 644
Hsp92I GRCGYC 1 cut(s) 432
Hsp92II CATG 7 cut(s) 98, 116, 145, 307, 365, 652, 1078
Kpn2I TCCGGA 1 cut(s) 1154
Ksp22I TGATCA 2 cut(s) 109, 1249
KspAI GTTAAC 1 cut(s) 598
Kzo9I GATC 9 cut(s) 109, 480, 495, 1173, 1195, 1201, 1228, 1249, 1476
LguI GCTCTTC 2 cut(s) 338, 1122
LmnI GCTCC 3 cut(s) 1018, 1295, 1313
Lsp1109I GCAGC 2 cut(s) 103, 1444
LweI GCATC 2 cut(s) 265, 1193
MaeI CTAG 5 cut(s) 231, 614, 714, 1245, 1358
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 4 cut(s) 344, 401, 1276, 1469
MalI GATC 9 cut(s) 111, 482, 497, 1175, 1197, 1203, 1230, 1251, 1478
MboI GATC 9 cut(s) 109, 480, 495, 1173, 1195, 1201, 1228, 1249, 1476
MfeI CAATTG 1 cut(s) 948
MflI RGATCY 2 cut(s) 1195, 1201
MhlI GDGCHC 6 cut(s) 300, 1015, 1107, 1323, 1400, 1502
MluCI AATT 9 cut(s) 77, 788, 948, 962, 1097, 1130, 1223, 1421, 1493
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 1 cut(s) 852
MroI TCCGGA 1 cut(s) 1154
MroXI GAANNNNTTC 3 cut(s) 168, 245, 332
MseI TTAA 7 cut(s) 543, 597, 708, 965, 1095, 1134, 1504
MslI CAYNNNNRTG 1 cut(s) 1372
MspI CCGG 9 cut(s) 134, 152, 204, 399, 412, 684, 1155, 1217, 1464
MspR9I CCNGG 4 cut(s) 135, 204, 684, 1311
MunI CAATTG 1 cut(s) 948
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 1 cut(s) 1311
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 4 cut(s) 104, 113, 710, 1322
NciI CCSGG 3 cut(s) 135, 204, 684
NcoI CCATGG 1 cut(s) 361
NdeI CATATG 1 cut(s) 1191
NdeII GATC 9 cut(s) 109, 480, 495, 1173, 1195, 1201, 1228, 1249, 1476
NlaIII CATG 7 cut(s) 98, 116, 145, 307, 365, 652, 1078
NlaIV GGNNCC 3 cut(s) 208, 688, 1197
NmuCI GTSAC 3 cut(s) 401, 1276, 1469
NspI RCATGY 2 cut(s) 98, 307
NspV TTCGAA 1 cut(s) 85
PciI ACATGT 1 cut(s) 303
PciSI GCTCTTC 2 cut(s) 338, 1122
PctI GAATGC 1 cut(s) 366
PdmI GAANNNNTTC 3 cut(s) 168, 245, 332
PfeI GAWTC 2 cut(s) 197, 771
PfoI TCCNGGA 2 cut(s) 202, 1309
PkrI GCNGC 4 cut(s) 118, 132, 1459, 1462
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
PscI ACATGT 1 cut(s) 303
Psp124BI GAGCTC 2 cut(s) 300, 1323
Psp6I CCWGG 1 cut(s) 1309
PspEI GGTNACC 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 883
PspGI CCWGG 1 cut(s) 1309
PspN4I GGNNCC 3 cut(s) 208, 688, 1197
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 2 cut(s) 1195, 1201
RsaI GTAC 5 cut(s) 42, 380, 647, 893, 1049
RsaNI GTAC 5 cut(s) 41, 379, 646, 892, 1048
RseI CAYNNNNRTG 1 cut(s) 1372
SacI GAGCTC 2 cut(s) 300, 1323
SapI GCTCTTC 2 cut(s) 338, 1122
SaqAI TTAA 7 cut(s) 543, 597, 708, 965, 1095, 1134, 1504
SatI GCNGC 4 cut(s) 117, 131, 1458, 1461
Sau3AI GATC 9 cut(s) 109, 480, 495, 1173, 1195, 1201, 1228, 1249, 1476
ScaI AGTACT 2 cut(s) 380, 1049
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 4 cut(s) 135, 204, 684, 1311
SduI GDGCHC 6 cut(s) 300, 1015, 1107, 1323, 1400, 1502
SfaNI GCATC 2 cut(s) 265, 1193
SfcI CTRYAG 1 cut(s) 1480
SfuI TTCGAA 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 1372
SmlI CTYRAG 3 cut(s) 179, 293, 589
SmoI CTYRAG 3 cut(s) 179, 293, 589
SpeI ACTAGT 1 cut(s) 1244
Sse9I AATT 9 cut(s) 77, 788, 948, 962, 1097, 1130, 1223, 1421, 1493
SsiI CCGC 4 cut(s) 131, 690, 1182, 1460
SspI AATATT 1 cut(s) 627
SspMI CTAG 5 cut(s) 231, 614, 714, 1245, 1358
SstI GAGCTC 2 cut(s) 300, 1323
StyD4I CCNGG 4 cut(s) 133, 202, 682, 1309
StyI CCWWGG 3 cut(s) 361, 699, 1017
TaaI ACNGT 4 cut(s) 394, 828, 1152, 1284
TaiI ACGT 1 cut(s) 647
TaqI TCGA 8 cut(s) 85, 483, 494, 500, 620, 731, 830, 1419
TasI AATT 9 cut(s) 77, 788, 948, 962, 1097, 1130, 1223, 1421, 1493
TatI WGTACW 3 cut(s) 40, 378, 1047
TauI GCSGC 2 cut(s) 133, 1463
TfiI GAWTC 2 cut(s) 197, 771
Tru1I TTAA 7 cut(s) 543, 597, 708, 965, 1095, 1134, 1504
Tru9I TTAA 7 cut(s) 543, 597, 708, 965, 1095, 1134, 1504
TscAI CASTG 3 cut(s) 60, 230, 482
TseFI GTSAC 3 cut(s) 401, 1276, 1469
TseI GCWGC 2 cut(s) 116, 1457
Tsp45I GTSAC 3 cut(s) 401, 1276, 1469
TspDTI ATGAA 6 cut(s) 430, 557, 867, 926, 1340, 1505
TspGWI ACGGA 2 cut(s) 781, 813
TspRI CASTG 3 cut(s) 60, 230, 482
VneI GTGCAC 1 cut(s) 1103
XapI RAATTY 3 cut(s) 1097, 1130, 1421
XceI RCATGY 2 cut(s) 98, 307
XmiI GTMKAC 2 cut(s) 91, 927
XmnI GAANNNNTTC 3 cut(s) 168, 245, 332
XspI CTAG 5 cut(s) 231, 614, 714, 1245, 1358
ZrmI AGTACT 2 cut(s) 380, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.