Rorug05G0556200

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005181
Physical Location & Seq
Reverse (-)
74448098 .. 74450686
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0556200.1

Sequence Viewer

Length: 1968 bp
ATGGTTGCAAAAGACCACCAGAGTGCTACAAATTCTCCTAGTCCAAAAGTGGAGGTGGGAGAGATCGACACCAGGGCACCTTTCCAATCTGTTAAAGATGCTGTGTCACTATTTGGTGAGGGGGCATTCTCTGGCTCTGGGGAGAAACCTGCCATTAGGAAGATAAAACCCCAGTCAGCGGAGCGAGTGCTAGCCAGTGAGACACAGCTTCACCTGGCCCAGAAAGAATTGAACAAGCTGAAGGATCAACTAATGAATGCCGAAACTACTAAAGCCCAAGCACTTGTGGAGCTTGCAAAAGCCAAAGTAACTCATGAAGAGCTGAACAAGAAGCTGGCATCTCTTGGTGAATCTAAGGAATCTGCAATAAAGGCATCAGAAGCTGCAAAACATCGGGCAAAGCAACTCGAAGAAGCAAACAATGGGAACCTAGCTGGAACTGATGGTGCTTGGAAGCAAGACTTGGAAACAGATAGAGCACAATACATGAATGTAATTACTGAACTTGATGCTGCAAAGCAAGAGTTGAGGAAAATCCGTCAGGACTGTGATGCATCCTTAGAAGCAAAAGTTGCTGCATTCAAGCAGGCAGCAGAAGCTGAAGATGCAGCCAAAGCAAATGCGGAGAGGGTTAATGAGCTCTCAAAGGAAATTGCAGCTGTGCATGAATCAATTGGGCAAGTGAAACTTGCATCTGAGGAAGCCCAGCAAGAGAAAGCAAAGGTATTTTCCGAAAAGGATGTCCTGAGGCAAGCATATAAGGCTACGGCAGAAGAGTCTGCAAAGAAATTGCTCTCTCTACAAAAAAAGTTTGATCCAGAAATTTCCAGAAATCTGGAAGCACAACTTGCTGATACATTGAATGAAATTGGAGCTCTGCAAAAGCAAATGGAGAATGAAAAGGCTTCAGATTTAGATTCTGTAAGAACGGTCACTTCGGAGCTGGATGATGCTAAGGAATCACTGCATAGAGTAGCCGAAGAAGAAAACAACCTCAGAAGTTTAGTTGAAGCACTTAAGGTGGAAATTGAGAATGTGAAGAAAGAGCATGCTGAGCTCAAGGAAAAGGAAGCGGAAACAGAATCTATTGCTGGGAACTTACATGTCAAACTGCGGAAAACTAAGACTGAGCTTGAAGCATGCCTAGCCGAAGAATCTAAAGCCAGAGGTGCTTCCAATGAAATGATAGCAACACTAAACCAGCTGTCGTTGGAAATTGAAAATGCTCGACGGGATGCAGAAGAGATGAAGATTAAAGCGGAGGAAATGAAAAAGGAAGCTGAAACTACCAAAAGTGCTGTAAAAGAGGCAGAGAAGAAGCTGAGAATTGCTCTGGAAGAAGCTGAAGAAGCTAAATCAGCAGAAGAAAGTGCCCTTGAACAGATTAGAACTTTATCCGAGAGAACTAACGCTGTTCGCGCATCAACATCTGAGTCTGGTGGCCAGATCACTATATCGAAGGATGAATTTGAGTCTTTGAGCCGAAAGGTTGAGGAGTCCGACACATTAGCAGAAATGAGAGTGGCTGCTGCCATGGCTCAGGTGGAAGCTGTGAAGGCTAGTGAAAATGAGGCCTTGAAGAAGTTAGAGGCAACCCAGAAGGAGATTGAAGATATGAAGGCTGCAACTGAGGAGGCCAAAAAGAGGGCCGAAATGGCTGAAGCAGCCAAAAAGGCAGTGGAAGGAGAGCTCCGAAGGTGGCGGGAACGCGAGCAAAAGAAAGCAGCAGATGCTGCCTCACGGATAATGGCAGAGACAGAAGAGTCAGTGGAATCATCCCCGCGCCATTACAGGATTCAAAAGCAGAACTCCCAGACGAAAACTGTCCAGGGCCGGAAGATGGAGAAAGCAAAGACCTCTGTTTCAAAAAAGACACTATTGCCTACCCTCAGCGGGATGTTTAATAGGAAAAGGAACCAGATTGAGGGTGGCTCTCCGTCTTATCTCCCTGGTGAGAGACCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

655

Amino Acids

72.38

Weight (kDa)

5.63

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 21 - 573 3.9e-56 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1762
Acc36I ACCTGC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 76
AccII CGCG 3 cut(s) 1419, 1710, 1783
AciI CCGC 8 cut(s) 179, 623, 1073, 1114, 1259, 1702, 1781, 1893
AclWI GGATC 2 cut(s) 252, 809
AcoI YGGCCR 1 cut(s) 1441
AcsI RAATTY 3 cut(s) 31, 822, 1466
AcuI CTGAAG 5 cut(s) 260, 621, 891, 1365, 1680
AfiI CCNNNNNNNGG 5 cut(s) 178, 1644, 1840, 1893, 1924
AflII CTTAAG 1 cut(s) 1016
AflIII ACRYGT 1 cut(s) 1102
AjnI CCWGG 4 cut(s) 71, 213, 1827, 1948
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AleI CACNNNNGTG 1 cut(s) 21
Alw21I GWGCWC 5 cut(s) 481, 642, 877, 1059, 1692
Alw26I GTCTC 3 cut(s) 194, 1748, 1951
AlwI GGATC 2 cut(s) 252, 809
AlwNI CAGNNNCTG 3 cut(s) 383, 599, 1733
AoxI GGCC 6 cut(s) 216, 1441, 1572, 1635, 1647, 1831
ApoI RAATTY 3 cut(s) 31, 822, 1466
ArsI GACNNNNNNTTYG 2 cut(s) 726, 758
AspLEI GCGC 2 cut(s) 1421, 1785
AspS9I GGNCC 3 cut(s) 217, 1647, 1831
AsuHPI GGTGA 4 cut(s) 128, 203, 359, 1964
AsuNHI GCTAGC 1 cut(s) 190
AxyI CCTNAGG 1 cut(s) 746
BaeGI GKGCMC 2 cut(s) 79, 1375
BalI TGGCCA 1 cut(s) 1443
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 4 cut(s) 642, 877, 1059, 1692
Bbv12I GWGCWC 5 cut(s) 481, 642, 877, 1059, 1692
BbvCI CCTCAGC 1 cut(s) 1889
BccI CCATC 2 cut(s) 437, 1834
BceAI ACGGC 1 cut(s) 783
BciT130I CCWGG 4 cut(s) 73, 215, 1829, 1950
BcoDI GTCTC 3 cut(s) 194, 1748, 1951
BfaI CTAG 5 cut(s) 39, 191, 431, 1145, 1560
BfrI CTTAAG 1 cut(s) 1016
BfuAI ACCTGC 1 cut(s) 157
BglI GCCNNNNNGGC 2 cut(s) 1655, 1673
BlpI GCTNAGC 1 cut(s) 1053
Bme1390I CCNGG 4 cut(s) 73, 215, 1829, 1950
BmgT120I GGNCC 3 cut(s) 217, 1647, 1831
BmiI GGNNCC 3 cut(s) 78, 428, 1916
BmrFI CCNGG 4 cut(s) 73, 215, 1829, 1950
BmrI ACTGGG 1 cut(s) 166
BmtI GCTAGC 1 cut(s) 194
BmuI ACTGGG 1 cut(s) 166
BplI GAGNNNNNCTC 4 cut(s) 1315, 1347, 1916, 1948
Bpu10I CCTNAGC 3 cut(s) 954, 1539, 1889
Bpu1102I GCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 1 cut(s) 1043
BsaI GGTCTC 1 cut(s) 1951
BsaJI CCNNGG 4 cut(s) 72, 1533, 1828, 1948
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 5 cut(s) 178, 1644, 1840, 1893, 1924
Bse1I ACTGG 2 cut(s) 172, 195
Bse21I CCTNAGG 1 cut(s) 746
BseBI CCWGG 4 cut(s) 73, 215, 1829, 1950
BseDI CCNNGG 4 cut(s) 72, 1533, 1828, 1948
BseGI GGATG 7 cut(s) 554, 745, 952, 1240, 1468, 1775, 1902
BseLI CCNNNNNNNGG 5 cut(s) 178, 1644, 1840, 1893, 1924
BseNI ACTGG 2 cut(s) 172, 195
BseRI GAGGAG 2 cut(s) 1508, 1646
BseSI GKGCMC 2 cut(s) 79, 1375
BseYI CCCAGC 2 cut(s) 705, 1091
Bsh1236I CGCG 3 cut(s) 1419, 1710, 1783
BshFI GGCC 6 cut(s) 218, 1443, 1574, 1637, 1649, 1833
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 5 cut(s) 481, 642, 877, 1059, 1692
BsiSI CCGG 1 cut(s) 1834
BslI CCNNNNNNNGG 5 cut(s) 178, 1644, 1840, 1893, 1924
BsmAI GTCTC 3 cut(s) 194, 1748, 1951
BsmI GAATGC 3 cut(s) 125, 262, 578
BsnI GGCC 6 cut(s) 218, 1443, 1574, 1637, 1649, 1833
Bso31I GGTCTC 1 cut(s) 1951
Bsp1286I GDGCHC 7 cut(s) 79, 481, 642, 877, 1059, 1375, 1692
Bsp143I GATC 4 cut(s) 63, 244, 814, 1446
Bsp1720I GCTNAGC 1 cut(s) 1053
Bsp19I CCATGG 1 cut(s) 1533
BspACI CCGC 8 cut(s) 179, 623, 1073, 1114, 1259, 1702, 1781, 1893
BspANI GGCC 6 cut(s) 218, 1443, 1574, 1637, 1649, 1833
BspFNI CGCG 3 cut(s) 1419, 1710, 1783
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 78, 428, 1916
BspMI ACCTGC 1 cut(s) 157
BspOI GCTAGC 1 cut(s) 194
BspPI GGATC 2 cut(s) 252, 809
BspQI GCTCTTC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 76
BspTI CTTAAG 1 cut(s) 1016
BspTNI GGTCTC 1 cut(s) 1951
BsrI ACTGG 2 cut(s) 172, 195
BssECI CCNNGG 4 cut(s) 72, 1533, 1828, 1948
BssMI GATC 4 cut(s) 63, 244, 814, 1446
BssT1I CCWWGG 1 cut(s) 1533
Bst2UI CCWGG 4 cut(s) 73, 215, 1829, 1950
Bst4CI ACNGT 3 cut(s) 548, 931, 1825
Bst6I CTCTTC 4 cut(s) 312, 768, 1236, 1755
BstAFI CTTAAG 1 cut(s) 1016
BstAPI GCANNNNNTGC 4 cut(s) 572, 848, 1730, 1733
BstC8I GCNNGC 8 cut(s) 192, 294, 336, 588, 753, 1050, 1141, 1712
BstDSI CCRYGG 1 cut(s) 1533
BstF5I GGATG 7 cut(s) 554, 745, 952, 1240, 1468, 1775, 1902
BstFNI CGCG 3 cut(s) 1419, 1710, 1783
BstHHI GCGC 2 cut(s) 1421, 1785
BstKTI GATC 4 cut(s) 66, 247, 817, 1449
BstMAI GTCTC 3 cut(s) 194, 1748, 1951
BstMBI GATC 4 cut(s) 63, 244, 814, 1446
BstNI CCWGG 4 cut(s) 73, 215, 1829, 1950
BstNSI RCATGY 3 cut(s) 1052, 1106, 1143
BstSCI CCNGG 4 cut(s) 71, 213, 1827, 1948
BstSLI GKGCMC 2 cut(s) 79, 1375
BstUI CGCG 3 cut(s) 1419, 1710, 1783
BstXI CCANNNNNNTGG 1 cut(s) 835
Bsu36I CCTNAGG 1 cut(s) 746
BsuRI GGCC 6 cut(s) 218, 1443, 1574, 1637, 1649, 1833
BtgI CCRYGG 1 cut(s) 1533
BtsCI GGATG 7 cut(s) 554, 745, 952, 1240, 1468, 1775, 1902
BtsI GCAGTG 2 cut(s) 962, 1683
BtsIMutI CAGTG 4 cut(s) 202, 962, 1683, 1773
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 8 cut(s) 192, 294, 336, 588, 753, 1050, 1141, 1712
CaiI CAGNNNCTG 3 cut(s) 383, 599, 1733
CciI TCATGA 1 cut(s) 313
CfoI GCGC 2 cut(s) 1421, 1785
Cfr13I GGNCC 3 cut(s) 217, 1647, 1831
CviAII CATG 7 cut(s) 314, 487, 665, 1049, 1103, 1140, 1534
DpnI GATC 4 cut(s) 65, 246, 816, 1448
DpnII GATC 4 cut(s) 63, 244, 814, 1446
DrdI GACNNNNNNGTC 1 cut(s) 1762
DseDI GACNNNNNNGTC 1 cut(s) 1762
EaeI YGGCCR 1 cut(s) 1441
Eam1104I CTCTTC 4 cut(s) 312, 768, 1236, 1755
EarI CTCTTC 4 cut(s) 312, 768, 1236, 1755
Ecl136II GAGCTC 4 cut(s) 640, 875, 1057, 1690
Eco130I CCWWGG 1 cut(s) 1533
Eco147I AGGCCT 1 cut(s) 1574
Eco24I GRGCYC 4 cut(s) 642, 877, 1059, 1692
Eco31I GGTCTC 1 cut(s) 1951
Eco53kI GAGCTC 4 cut(s) 640, 875, 1057, 1690
Eco57I CTGAAG 5 cut(s) 260, 621, 891, 1365, 1680
Eco81I CCTNAGG 1 cut(s) 746
EcoICRI GAGCTC 4 cut(s) 640, 875, 1057, 1690
EcoRII CCWGG 4 cut(s) 71, 213, 1827, 1948
EcoT14I CCWWGG 1 cut(s) 1533
EcoT22I ATGCAT 1 cut(s) 556
EcoT38I GRGCYC 4 cut(s) 642, 877, 1059, 1692
ErhI CCWWGG 1 cut(s) 1533
FaeI CATG 7 cut(s) 317, 490, 668, 1052, 1106, 1143, 1537
FalI AAGNNNNNCTT 6 cut(s) 446, 478, 672, 704, 831, 863
FatI CATG 7 cut(s) 313, 486, 664, 1048, 1102, 1139, 1533
FauI CCCGC 3 cut(s) 1695, 1788, 1886
FokI GGATG 7 cut(s) 541, 752, 959, 1247, 1475, 1762, 1909
FriOI GRGCYC 4 cut(s) 642, 877, 1059, 1692
FspBI CTAG 5 cut(s) 39, 191, 431, 1145, 1560
GlaI GCGC 2 cut(s) 1420, 1784
GsaI CCCAGC 2 cut(s) 709, 1095
HaeIII GGCC 6 cut(s) 218, 1443, 1574, 1637, 1649, 1833
HapII CCGG 1 cut(s) 1834
HhaI GCGC 2 cut(s) 1421, 1785
Hin1II CATG 7 cut(s) 317, 490, 668, 1052, 1106, 1143, 1537
Hin6I GCGC 2 cut(s) 1419, 1783
HinP1I GCGC 2 cut(s) 1419, 1783
HpaII CCGG 1 cut(s) 1834
HphI GGTGA 4 cut(s) 128, 203, 359, 1964
Hpy188III TCNNGA 7 cut(s) 314, 542, 745, 818, 828, 836, 1334
Hpy99I CGWCG 1 cut(s) 1233
HpyAV CCTTC 7 cut(s) 235, 1453, 1549, 1594, 1612, 1676, 1689
HpyCH4III ACNGT 3 cut(s) 548, 931, 1825
Hsp92II CATG 7 cut(s) 317, 490, 668, 1052, 1106, 1143, 1537
HspAI GCGC 2 cut(s) 1419, 1783
Kzo9I GATC 4 cut(s) 63, 244, 814, 1446
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 5 cut(s) 181, 289, 872, 940, 1695
MaeI CTAG 5 cut(s) 39, 191, 431, 1145, 1560
MaeIII GTNAC 3 cut(s) 105, 307, 931
MalI GATC 4 cut(s) 65, 246, 816, 1448
MboI GATC 4 cut(s) 63, 244, 814, 1446
MfeI CAATTG 1 cut(s) 672
MhlI GDGCHC 7 cut(s) 79, 481, 642, 877, 1059, 1375, 1692
MlsI TGGCCA 1 cut(s) 1443
MluNI TGGCCA 1 cut(s) 1443
MlyI GAGTC 5 cut(s) 785, 1442, 1481, 1505, 1772
MmeI TCCRAC 2 cut(s) 1191, 1524
Mox20I TGGCCA 1 cut(s) 1443
Mph1103I ATGCAT 1 cut(s) 556
MscI TGGCCA 1 cut(s) 1443
MseI TTAA 5 cut(s) 93, 633, 1017, 1254, 1902
MslI CAYNNNNRTG 1 cut(s) 21
Msp20I TGGCCA 1 cut(s) 1443
MspA1I CMGCKG 4 cut(s) 179, 659, 1204, 1893
MspCI CTTAAG 1 cut(s) 1016
MspI CCGG 1 cut(s) 1834
MspR9I CCNGG 4 cut(s) 73, 215, 1829, 1950
MunI CAATTG 1 cut(s) 672
Mva1269I GAATGC 3 cut(s) 125, 262, 578
MvaI CCWGG 4 cut(s) 73, 215, 1829, 1950
MvnI CGCG 3 cut(s) 1419, 1710, 1783
NcoI CCATGG 1 cut(s) 1533
NdeII GATC 4 cut(s) 63, 244, 814, 1446
NheI GCTAGC 1 cut(s) 190
NlaIII CATG 7 cut(s) 317, 490, 668, 1052, 1106, 1143, 1537
NlaIV GGNNCC 3 cut(s) 78, 428, 1916
NmuCI GTSAC 2 cut(s) 105, 931
NsiI ATGCAT 1 cut(s) 556
NspI RCATGY 3 cut(s) 1052, 1106, 1143
OliI CACNNNNGTG 1 cut(s) 21
PaeI GCATGC 2 cut(s) 1052, 1143
PagI TCATGA 1 cut(s) 313
PceI AGGCCT 1 cut(s) 1574
PciI ACATGT 1 cut(s) 1102
PciSI GCTCTTC 1 cut(s) 312
PctI GAATGC 3 cut(s) 125, 262, 578
PfeI GAWTC 9 cut(s) 350, 359, 668, 917, 959, 1082, 1154, 1772, 1795
PleI GAGTC 5 cut(s) 784, 1441, 1480, 1504, 1771
PpsI GAGTC 5 cut(s) 784, 1441, 1480, 1504, 1771
PscI ACATGT 1 cut(s) 1102
Psp124BI GAGCTC 4 cut(s) 642, 877, 1059, 1692
Psp6I CCWGG 4 cut(s) 71, 213, 1827, 1948
PspFI CCCAGC 2 cut(s) 705, 1091
PspGI CCWGG 4 cut(s) 71, 213, 1827, 1948
PspN4I GGNNCC 3 cut(s) 78, 428, 1916
PspPI GGNCC 3 cut(s) 217, 1647, 1831
PstNI CAGNNNCTG 3 cut(s) 383, 599, 1733
PvuII CAGCTG 2 cut(s) 659, 1204
RseI CAYNNNNRTG 1 cut(s) 21
SacI GAGCTC 4 cut(s) 642, 877, 1059, 1692
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 5 cut(s) 93, 633, 1017, 1254, 1902
Sau3AI GATC 4 cut(s) 63, 244, 814, 1446
Sau96I GGNCC 3 cut(s) 217, 1647, 1831
SchI GAGTC 5 cut(s) 785, 1442, 1481, 1505, 1772
ScrFI CCNGG 4 cut(s) 73, 215, 1829, 1950
SduI GDGCHC 7 cut(s) 79, 481, 642, 877, 1059, 1375, 1692
SmiMI CAYNNNNRTG 1 cut(s) 21
SmlI CTYRAG 2 cut(s) 1016, 1058
SmoI CTYRAG 2 cut(s) 1016, 1058
SphI GCATGC 2 cut(s) 1052, 1143
SseBI AGGCCT 1 cut(s) 1574
SsiI CCGC 8 cut(s) 179, 623, 1073, 1114, 1259, 1702, 1781, 1893
SspMI CTAG 5 cut(s) 39, 191, 431, 1145, 1560
SstI GAGCTC 4 cut(s) 642, 877, 1059, 1692
StuI AGGCCT 1 cut(s) 1574
StyD4I CCNGG 4 cut(s) 71, 213, 1827, 1948
StyI CCWWGG 1 cut(s) 1533
TaaI ACNGT 3 cut(s) 548, 931, 1825
TaqI TCGA 4 cut(s) 66, 408, 1228, 1457
TfiI GAWTC 9 cut(s) 350, 359, 668, 917, 959, 1082, 1154, 1772, 1795
Tru1I TTAA 5 cut(s) 93, 633, 1017, 1254, 1902
Tru9I TTAA 5 cut(s) 93, 633, 1017, 1254, 1902
TscAI CASTG 4 cut(s) 202, 969, 1683, 1773
TseFI GTSAC 2 cut(s) 105, 931
Tsp45I GTSAC 2 cut(s) 105, 931
TspGWI ACGGA 3 cut(s) 527, 1756, 1926
TspRI CASTG 4 cut(s) 202, 969, 1683, 1773
Vha464I CTTAAG 1 cut(s) 1016
XapI RAATTY 3 cut(s) 31, 822, 1466
XceI RCATGY 3 cut(s) 1052, 1106, 1143
XcmI CCANNNNNNNNNTGG 3 cut(s) 1540, 1675, 1925
XspI CTAG 5 cut(s) 39, 191, 431, 1145, 1560
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.