Rorug06G0243200

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005181
Physical Location & Seq
Forward (+)
38707596 .. 38711024
3429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0243200.1

Sequence Viewer

Length: 1131 bp
ATGAGCGTCCCCGGAAAGCTGAAATCCTCGAGCTCCGAATTGGACCTGGATCGGCCCAACCTCGAGGACTACCTCCCTTCCGGAGCCTCCATCCAGCAAGAACCCGACGGCAACCTTCGCCTGCGTGATTTGCTTGATATCTCTCCCGCCCTCGCCGAGGCCGCCGGTGCCATTATCGATGACTCGTTCACGCGGTGCTTTAAGTCGAATCCTCCGGAGCCGTGGAATTGGAATATCTATTTGTTCCCTTTGTGGTGTTTCGGAGTGGTGATTCGATACTTTGTTCTATTTCCGGCAAGGGTACTGGTCTTGACTATTGGATGGATAATTTTCCTTTCGGCATTCATTCCTGTGCACTTCCTACTCAAAGGCCATGAAAAATGGAGAAAAAATTTAGAGAGATGTTTGGTGGAGTTAATGTGTAGCTTCTTTGTTGCATCTTGGACTGGGGTTGTGAAGTACCATGGACCAAAGCCTAGTATGCGGCCAAAGCAGGTTTTTGTGGCCAATCATACTTCTATGATTGATTTCATCATCCTGGAACAAATGACCGCATTTGCTGTAATTATGCAAAAGCATCCTGGATGGGTTGGATTGTTGCAAAAAACTATTTTGGAGAGTGTGGGATGTATCTGGTTCAATCGTTCAGAATCAAAGGATCGAGAAATTGTTGCGAAGAAATTAAGGGATCATGTGGTGGGGCCTGACAATAACCCTCTCCTTATATTTCCCGAAGGAACTTGTGTAAACAATCACTACACTGTTATGTTTAAGAAGGGTGCATTTGAGCTTGGCTGCTCAGTTTGCCCTGTTGCAATCAAGTACAATAAAATTTTCGTTGATGCATTCTGGAACAGCAGAAAGCAGTCCTTCACAATGCATCTGCTACAGCTTATGACATCATGGGCTGTTGTTTGTGATGTGTGGTATTTGGAGCCCGAAAATATGAAGCCTGGGGAGACTGCCATTGAATTTGCAGAGAGGGTCAGGGACATAATTTCTGTTCGAGCAGGACTGAAGAAGGTCCCGTGGGATGGGTATCTGAAATACTCTCGCCCTAGCCCAAAGCATAGAGAGAGGAAGCAACAGAGCTTTGCCGAGTCTATGCTCCGACGCCTCGAGGATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

376

Amino Acids

43.25

Weight (kDa)

8.94

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 152 - 274 4.1e-25 Acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 2 cut(s) 62, 1118
Acc36I ACCTGC 1 cut(s) 484
AccB1I GGYRCC 1 cut(s) 167
AccII CGCG 1 cut(s) 193
AccIII TCCGGA 2 cut(s) 80, 214
AciI CCGC 5 cut(s) 147, 162, 193, 484, 552
AclWI GGATC 3 cut(s) 57, 666, 696
AcoI YGGCCR 2 cut(s) 485, 504
AcsI RAATTY 3 cut(s) 391, 831, 971
AcuI CTGAAG 1 cut(s) 1037
AcyI GRCGYC 1 cut(s) 1114
AdeI CACNNNGTG 1 cut(s) 195
AfaI GTAC 3 cut(s) 303, 461, 824
AfiI CCNNNNNNNGG 2 cut(s) 157, 1034
AgsI TTSAA 2 cut(s) 640, 971
AjnI CCWGG 4 cut(s) 45, 537, 580, 952
AluBI AGCT 6 cut(s) 19, 33, 426, 790, 892, 1092
AluI AGCT 6 cut(s) 19, 33, 426, 790, 892, 1092
Alw21I GWGCWC 2 cut(s) 35, 357
Alw26I GTCTC 1 cut(s) 953
Alw44I GTGCAC 1 cut(s) 353
AlwI GGATC 3 cut(s) 57, 666, 696
Ama87I CYCGRG 3 cut(s) 28, 62, 1118
Aor13HI TCCGGA 2 cut(s) 80, 214
AoxI GGCC 6 cut(s) 53, 159, 370, 485, 504, 701
ApaLI GTGCAC 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 795
ApoI RAATTY 3 cut(s) 391, 831, 971
AspS9I GGNCC 5 cut(s) 43, 54, 467, 701, 1024
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 280
AvaI CYCGRG 3 cut(s) 28, 62, 1118
AvaII GGWCC 3 cut(s) 43, 467, 1024
BaeGI GKGCMC 1 cut(s) 357
BalI TGGCCA 1 cut(s) 506
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 2 cut(s) 35, 939
Bbv12I GWGCWC 2 cut(s) 35, 357
BbvI GCAGC 1 cut(s) 782
BccI CCATC 4 cut(s) 98, 315, 579, 1028
BceAI ACGGC 2 cut(s) 124, 205
BciT130I CCWGG 4 cut(s) 47, 539, 582, 954
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 1 cut(s) 953
BfaI CTAG 2 cut(s) 477, 1059
BfmI CTRYAG 1 cut(s) 887
BfuAI ACCTGC 1 cut(s) 484
BisI GCNGC 3 cut(s) 162, 485, 796
BlsI GCNGC 3 cut(s) 163, 486, 797
Bme1390I CCNGG 5 cut(s) 12, 47, 539, 582, 954
Bme18I GGWCC 3 cut(s) 43, 467, 1024
BmeT110I CYCGRG 3 cut(s) 28, 62, 1118
BmgT120I GGNCC 5 cut(s) 43, 54, 467, 701, 1024
BmiI GGNNCC 6 cut(s) 85, 169, 219, 702, 936, 1026
BmrFI CCNGG 5 cut(s) 12, 47, 539, 582, 954
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 4 cut(s) 446, 586, 832, 889
BmuI ACTGGG 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 12
Bsa29I ATCGAT 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 1038
BsaHI GRCGYC 1 cut(s) 1114
BsaJI CCNNGG 6 cut(s) 10, 156, 221, 463, 953, 1028
BsaWI WCCGGW 2 cut(s) 80, 214
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 1034
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 309, 451
Bse8I GATNNNNATC 1 cut(s) 1038
BseAI TCCGGA 2 cut(s) 80, 214
BseBI CCWGG 4 cut(s) 47, 539, 582, 954
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 6 cut(s) 10, 156, 221, 463, 953, 1028
BseGI GGATG 7 cut(s) 90, 326, 534, 577, 590, 632, 1039
BseJI GATNNNNATC 1 cut(s) 1038
BseLI CCNNNNNNNGG 2 cut(s) 157, 1034
BseMII CTCAG 1 cut(s) 813
BseNI ACTGG 2 cut(s) 309, 451
BseSI GKGCMC 1 cut(s) 357
BseXI GCAGC 1 cut(s) 782
Bsh1236I CGCG 1 cut(s) 193
BshFI GGCC 6 cut(s) 55, 161, 372, 487, 506, 703
BshNI GGYRCC 1 cut(s) 167
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 35, 357
BsiHKCI CYCGRG 3 cut(s) 28, 62, 1118
BsiSI CCGG 5 cut(s) 12, 81, 165, 215, 293
BslFI GGGAC 2 cut(s) 1004, 1010
BslI CCNNNNNNNGG 2 cut(s) 157, 1034
BsmAI GTCTC 1 cut(s) 953
BsmFI GGGAC 2 cut(s) 1004, 1010
BsmI GAATGC 2 cut(s) 341, 845
BsnI GGCC 6 cut(s) 55, 161, 372, 487, 506, 703
BsoBI CYCGRG 3 cut(s) 28, 62, 1118
Bsp1286I GDGCHC 3 cut(s) 35, 357, 939
Bsp13I TCCGGA 2 cut(s) 80, 214
Bsp143I GATC 3 cut(s) 49, 658, 688
Bsp19I CCATGG 1 cut(s) 463
BspACI CCGC 5 cut(s) 147, 162, 193, 484, 552
BspANI GGCC 6 cut(s) 55, 161, 372, 487, 506, 703
BspCNI CTCAG 1 cut(s) 812
BspDI ATCGAT 1 cut(s) 177
BspEI TCCGGA 2 cut(s) 80, 214
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 6 cut(s) 85, 169, 219, 702, 936, 1026
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 3 cut(s) 57, 666, 696
BspT107I GGYRCC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 2 cut(s) 309, 451
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 6 cut(s) 10, 156, 221, 463, 953, 1028
BssMI GATC 3 cut(s) 49, 658, 688
BssNI GRCGYC 1 cut(s) 1114
BssT1I CCWWGG 1 cut(s) 463
Bst2UI CCWGG 4 cut(s) 47, 539, 582, 954
Bst4CI ACNGT 1 cut(s) 763
BstACI GRCGYC 1 cut(s) 1114
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 1 cut(s) 799
BstDSI CCRYGG 3 cut(s) 221, 463, 1028
BstENI CCTNNNNNAGG 1 cut(s) 155
BstF5I GGATG 7 cut(s) 90, 326, 534, 577, 590, 632, 1039
BstFNI CGCG 1 cut(s) 193
BstKTI GATC 3 cut(s) 52, 661, 691
BstMAI GTCTC 1 cut(s) 953
BstMBI GATC 3 cut(s) 49, 658, 688
BstMWI GCNNNNNNNGC 7 cut(s) 117, 130, 161, 167, 481, 490, 804
BstNI CCWGG 4 cut(s) 47, 539, 582, 954
BstSCI CCNGG 5 cut(s) 10, 45, 537, 580, 952
BstSFI CTRYAG 1 cut(s) 887
BstSLI GKGCMC 1 cut(s) 357
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 782
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 6 cut(s) 55, 161, 372, 487, 506, 703
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 3 cut(s) 221, 463, 1028
BtsCI GGATG 7 cut(s) 90, 326, 534, 577, 590, 632, 1039
BtsIMutI CAGTG 1 cut(s) 759
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 5 cut(s) 43, 54, 467, 701, 1024
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 1122
Csp6I GTAC 3 cut(s) 302, 460, 823
CviAII CATG 4 cut(s) 374, 464, 692, 903
CviQI GTAC 3 cut(s) 302, 460, 823
DdeI CTNAG 1 cut(s) 799
DpnI GATC 3 cut(s) 51, 660, 690
DpnII GATC 3 cut(s) 49, 658, 688
DraIII CACNNNGTG 1 cut(s) 195
EaeI YGGCCR 2 cut(s) 485, 504
Ecl136II GAGCTC 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 463
Eco24I GRGCYC 2 cut(s) 35, 939
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 3 cut(s) 43, 467, 1024
Eco53kI GAGCTC 1 cut(s) 33
Eco57I CTGAAG 1 cut(s) 1037
Eco88I CYCGRG 3 cut(s) 28, 62, 1118
EcoICRI GAGCTC 1 cut(s) 33
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoO109I RGGNCCY 2 cut(s) 701, 1024
EcoRII CCWGG 4 cut(s) 45, 537, 580, 952
EcoRV GATATC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 463
EcoT22I ATGCAT 2 cut(s) 847, 882
EcoT38I GRGCYC 2 cut(s) 35, 939
ErhI CCWWGG 1 cut(s) 463
FaeI CATG 4 cut(s) 377, 467, 695, 906
FalI AAGNNNNNCTT 2 cut(s) 854, 886
FaqI GGGAC 2 cut(s) 1004, 1010
FatI CATG 4 cut(s) 373, 463, 691, 902
FauI CCCGC 1 cut(s) 154
Fnu4HI GCNGC 3 cut(s) 162, 485, 796
FokI GGATG 7 cut(s) 77, 333, 521, 564, 597, 639, 1046
FriOI GRGCYC 2 cut(s) 35, 939
Fsp4HI GCNGC 3 cut(s) 162, 485, 796
FspBI CTAG 2 cut(s) 477, 1059
GluI GCNGC 3 cut(s) 162, 485, 796
HaeIII GGCC 6 cut(s) 55, 161, 372, 487, 506, 703
HapII CCGG 5 cut(s) 12, 81, 165, 215, 293
HgaI GACGC 1 cut(s) 1122
Hin1I GRCGYC 1 cut(s) 1114
Hin1II CATG 4 cut(s) 377, 467, 695, 906
HinfI GANTC 5 cut(s) 182, 208, 271, 650, 1100
HpaII CCGG 5 cut(s) 12, 81, 165, 215, 293
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 3 cut(s) 189, 355, 748
Hpy188I TCNGA 5 cut(s) 37, 263, 649, 1044, 1112
Hpy188III TCNNGA 6 cut(s) 81, 215, 310, 662, 731, 850
Hpy8I GTNNAC 3 cut(s) 189, 355, 748
Hpy99I CGWCG 2 cut(s) 110, 1116
HpyAV CCTTC 6 cut(s) 87, 125, 728, 769, 880, 1015
HpyCH4III ACNGT 1 cut(s) 763
HpyCH4V TGCA 9 cut(s) 355, 437, 571, 601, 782, 815, 845, 880, 977
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 130, 161, 167, 481, 490, 804
HpyF3I CTNAG 1 cut(s) 799
Hsp92I GRCGYC 1 cut(s) 1114
Hsp92II CATG 4 cut(s) 377, 467, 695, 906
Kpn2I TCCGGA 2 cut(s) 80, 214
Kzo9I GATC 3 cut(s) 49, 658, 688
LmnI GCTCC 5 cut(s) 38, 83, 217, 934, 1113
Lsp1109I GCAGC 1 cut(s) 782
LweI GCATC 4 cut(s) 446, 586, 832, 889
MaeI CTAG 2 cut(s) 477, 1059
MalI GATC 3 cut(s) 51, 660, 690
MboI GATC 3 cut(s) 49, 658, 688
MboII GAAGA 2 cut(s) 688, 1030
MhlI GDGCHC 3 cut(s) 35, 357, 939
MlsI TGGCCA 1 cut(s) 506
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 2 cut(s) 176, 1109
MmeI TCCRAC 1 cut(s) 571
Mox20I TGGCCA 1 cut(s) 506
Mph1103I ATGCAT 2 cut(s) 847, 882
MroI TCCGGA 2 cut(s) 80, 214
MscI TGGCCA 1 cut(s) 506
MseI TTAA 4 cut(s) 201, 416, 683, 771
MslI CAYNNNNRTG 2 cut(s) 350, 764
Msp20I TGGCCA 1 cut(s) 506
MspI CCGG 5 cut(s) 12, 81, 165, 215, 293
MspR9I CCNGG 5 cut(s) 12, 47, 539, 582, 954
Mva1269I GAATGC 2 cut(s) 341, 845
MvaI CCWGG 4 cut(s) 47, 539, 582, 954
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 7 cut(s) 117, 130, 161, 167, 481, 490, 804
NciI CCSGG 1 cut(s) 12
NcoI CCATGG 1 cut(s) 463
NdeII GATC 3 cut(s) 49, 658, 688
NlaIII CATG 4 cut(s) 377, 467, 695, 906
NlaIV GGNNCC 6 cut(s) 85, 169, 219, 702, 936, 1026
NmeAIII GCCGAG 2 cut(s) 181, 1123
NsiI ATGCAT 2 cut(s) 847, 882
PaeR7I CTCGAG 3 cut(s) 28, 62, 1118
PctI GAATGC 2 cut(s) 341, 845
PfeI GAWTC 3 cut(s) 208, 271, 650
PfoI TCCNGGA 2 cut(s) 537, 580
PkrI GCNGC 3 cut(s) 163, 486, 797
PleI GAGTC 2 cut(s) 176, 1108
PpsI GAGTC 2 cut(s) 176, 1108
PpuMI RGGWCCY 1 cut(s) 1024
Psp124BI GAGCTC 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 1024
Psp6I CCWGG 4 cut(s) 45, 537, 580, 952
PspGI CCWGG 4 cut(s) 45, 537, 580, 952
PspN4I GGNNCC 6 cut(s) 85, 169, 219, 702, 936, 1026
PspPI GGNCC 5 cut(s) 43, 54, 467, 701, 1024
PspPPI RGGWCCY 1 cut(s) 1024
PspXI VCTCGAGB 3 cut(s) 28, 62, 1118
RsaI GTAC 3 cut(s) 303, 461, 824
RsaNI GTAC 3 cut(s) 302, 460, 823
RseI CAYNNNNRTG 2 cut(s) 350, 764
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 4 cut(s) 201, 416, 683, 771
SatI GCNGC 3 cut(s) 162, 485, 796
Sau3AI GATC 3 cut(s) 49, 658, 688
Sau96I GGNCC 5 cut(s) 43, 54, 467, 701, 1024
SchI GAGTC 2 cut(s) 176, 1109
ScrFI CCNGG 5 cut(s) 12, 47, 539, 582, 954
SduI GDGCHC 3 cut(s) 35, 357, 939
SfaNI GCATC 4 cut(s) 446, 586, 832, 889
SfcI CTRYAG 1 cut(s) 887
Sfr274I CTCGAG 3 cut(s) 28, 62, 1118
SgrAI CRCCGGYG 1 cut(s) 164
SinI GGWCC 3 cut(s) 43, 467, 1024
SlaI CTCGAG 3 cut(s) 28, 62, 1118
SmiMI CAYNNNNRTG 2 cut(s) 350, 764
SmlI CTYRAG 3 cut(s) 28, 62, 1118
SmoI CTYRAG 3 cut(s) 28, 62, 1118
SsiI CCGC 5 cut(s) 147, 162, 193, 484, 552
SspMI CTAG 2 cut(s) 477, 1059
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 5 cut(s) 10, 45, 537, 580, 952
StyI CCWWGG 1 cut(s) 463
TaaI ACNGT 1 cut(s) 763
TaqI TCGA 8 cut(s) 29, 63, 177, 206, 274, 661, 1006, 1119
TatI WGTACW 1 cut(s) 822
TauI GCSGC 2 cut(s) 164, 487
TfiI GAWTC 3 cut(s) 208, 271, 650
Tru1I TTAA 4 cut(s) 201, 416, 683, 771
Tru9I TTAA 4 cut(s) 201, 416, 683, 771
TscAI CASTG 1 cut(s) 766
TseI GCWGC 1 cut(s) 795
TspDTI ATGAA 4 cut(s) 334, 390, 520, 962
TspRI CASTG 1 cut(s) 766
VneI GTGCAC 1 cut(s) 353
VpaK11BI GGWCC 3 cut(s) 43, 467, 1024
XagI CCTNNNNNAGG 1 cut(s) 155
XapI RAATTY 3 cut(s) 391, 831, 971
XhoI CTCGAG 3 cut(s) 28, 62, 1118
XspI CTAG 2 cut(s) 477, 1059
Zsp2I ATGCAT 2 cut(s) 847, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.