Rorug07G0201200

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003076
Physical Location & Seq
Forward (+)
17474857 .. 17483698
8842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0201200.1

Sequence Viewer

Length: 3573 bp
ATGGAAATTCTTGCTGCAATTCCCGGAAAACTTGCCGAGTTCACAGTTGAACCAATTGGACGCCAAGTGGGTTATCTAGCTCACTACAAAAGCAACCATCAAAAGCTGCAGACCAAATTGAAGAAACTAATTGCTGTCAGAGATGGGGTTCAACATAAGGTTGATCAAGTTGAGAGAGGAGGAAAAAGAATCGAGGCTACTGTTCTGAACTGGAAGACAGATGTGGATGCAATCATCACAGAGGCAGAGGAATTCCTGAGAGACGGCCGCCATGCGCAGACCGAGTGTCTTCATGGTTTTTGTCCTCATTTGGTGCTTCGTTATCGACTAAGCAGGAAATCAACAAAATTGGCGGAGGAGATTGATCAGCTTTATGGAAAAATTGATGAACTTGATAAAAAAAAGGAGTTCTCCAACTTTGCATATGATGTTCTTCCACAAGATTTATGTCTTTTATCTACCGGGCATTACAGTACATTCGATTCAAGGAATTCAGTTGTTTTGAGAGTCATGGATGAACTGAGAAATCCTGATACAGAAATGATTCTGGTATGGGGAATCGGTGGAGTAGGGAAGACCACACTCGTTGAAGAAGTTCTTAGGCAAGCTAAGGATGTTAACTTATTTGATGATTTGGTGATGGTGAGAGCTGTGAAAGAGAACCCAGACCTTGAAATAATCCAAAAAGAAATTGCCATGAAGTTGGGTATGAGCATTGACGAAAAGCATACTCTAGCTCAAAGAGCGCCCCTTCTAGGTGATAGGATAAAACAGAAAACGACTCTTGTAGTTTTAGACGATGTTTGGGAAAAAATTGATCTACTGGCTGTCGGAGTTCCCCGTCTGGTGAACCTTAAAGTATTGTTAACATGTAGAAGCCGAAAGGAGTTATCATTGGATATTCGAACACAGAAAGATATTTCGGTTGATGTTTTAAATGAAAAGGAAGCATGGAGTTTATTTGAGAAGATGGCTGGTGATGTTGTCAAAGATAGCCTCATACGAAGCGTAGCAATTGAAGTAGCCAAAAAATGTGGAGGTTTGCCGGTCTTGATTGTTACGGTTGCAAGAGCTCTAAAAGACGGAAACAAGTTGCAAGTATGGAAAGACACCTTGAGACGTCTTAGAGGCTTTGACAACAAAGAATTGACGGAAAAAGCATACTCGGCTCTGGAGTGGAGTTATGATCAATTGGAAGATAAAGAGCTTAAGCCGTTGTTCTTGCTTTGTGGGATTATTGTAGTGAAGAACCATATTAATTTGGCAGACTTGTTGAAATATGGTTTTGGTTTGGGTTTGATTAAAAATGTCGATACAATAGAAGAAGCACGAGATGCATTGGTTACACAAATTGAAAAGCTTAAAGATTCTTGCTTGTTGCTGGACAGTGATATCAATATGCATGTTAGAATGCATGATCTTGTATGTGAGGTTGCTACCCGGATTGCATCCAGAGATCAACATGTCTTATCAGTTGGATATGGAGGTGAACTAAAAGAATGGCCTGATAAAGATTTCTTTGAAAGGTGCAGTAAGATGTCTTTAAAATCCAGCAGTATTCCCATGCTTTCTGAAGTACCTTGGAAATGCCCGAAACTAGAGATGTTTCATTTGTATAGAAAAGGCCTGGGTGCTGCATCGGTGGAATTCCCTTCCAACTTTTTTAGTGAAATGTTGAAACTGAAAGTGTTGGGTTTAACTGCAGTGGATATGGTGTCACTACCTTCATCTCTTCAGTTCTTAAAGAATGTTCAGACTTTGTGTTTAGAAAACTGCGAGTTGGAAGATGTAGCTCTAGTTGGACATCTAACCAATTTGGAGATTCTTAGCTTTGCGCAGTCCAACATTAAACAGCTACCCAAAGAAATAGGGCAATTGATTCGATTGAGATGGTTGGATCTGAGTGATTGTTCCCAACTTGAAGTGATTTCACCTAATGTTATTTCAAGGTTGGAAAGACTAGGAGACTTGGCAATGAGAAACGTCTTTCTCAAATGGGAGGCTGAAAGGGGAGGAAGTAATGCTAGTCTTTTAGAGCTAAAAGACTTGTCTCAACTAACTGCATTGGAGATATATATTGAAGATGCAAATATTCTTCCATCAAACTTGTTCTCCAGTAGGTTGGTAAGATACCAAATATATATTGGCAATGTTTGGGAGCACAGATATCAAAGAAAGAAGACTATGGCTGAAACGACCCTCAACACGCTAAAACTGAAGCTCGCTCCCAGCGATGAATTGGACCACGGTATAAAAACATTGTTGAAGAAATCTGAAGATTTGTCTTTGGATGGAACAGAGAGTGTTAATAGTATTATGCGTCAATTAGATGTTGAAGATTTTCAGCAACTGATACACCTCCGTCTCCAAAATAATGTTGATTTCACCTATGTCATTAATAGGAAGGTTGTGTTCCCCAACTTAACAACCTTGAGCATATGTAGCTGTGATCATCTAAGTTTCTTGTTCTCATTTTCTATGGCTAAAAGTTTGGTGGAGCTCAAACATCTTGAAGTATCCAGATGTGCAATAATGGAAGAGATAGTATCAGCAAGAGATCAATCCAATGGAGAAAATATCGATATCATGTTTGGTAAGTTGGAAAGTCTAAAACTGGTTGAACTCCCAAACCTCGCTAAGTTCTATTCATGGATTCATGCTTCCAAGTGTCCGTTACTCAAAAAACTGACTATGTTTGGATGTGGGAAAGTGAAGATGTTTGCCGCAGAACGTCCAATTTTTCCAGAAAAACATCTAATTGAACAATCTCTCTTTCTAATTGACAAGGATTCATTCCCCAATTTAGAAGTCTTAACCCTGGAAGTTGCGGGTGAGATTTGGTACGGTCCATCATCCCCAGCACACTTCTTTCCCAAATTAAAAACTATTTACTTTTACGACTGGGCACGGATCTCCAAGCAAGCCGCAATACATTTCCTTGAGAAATTACCGAATCTTGAGGAGATTGGAGTGGGCTGGGATGATGTTTCCACGGAAATATTTGTGAATGAAGAAATTAATGGCGGTGAGGAGATACATGCAGTAAAAAATTTGGCGGTCATTGGCATGCCCAAGCTCTTGCAATTAGGGAATGGGAACTCTCAAACAGCGGGCCCACTTTTCCCAAACTTGGACACTCTTGGGATATATACTTGTAATCTGTTAAAGAGTGTAGAGTCATCCGCAGCAATATCCTTCCGCAATCTAACAACTCTGATGGTAGCGAGTTGTCATAGATTGGAATACTTGACAAGTTATTCTGTAGCTAAGAGCTTAATGCAACTGACAACACTGGGTGTGAGTTGGTGTGGAAGCCTGAGAGCAATTATAGGGGCAAGCAACGAAGATGATCATGATACAGGAGCAACTTGTGAGATTGCTTTCACCCGGTTGCAACATTTGACACTTGTGACGCTACCAAGACTGCAAGGCTTTTGCTCGGGAAATTGCATTGTCAGACTCCCATCCTCAACAGTGTTAGATGTCAGAGACTGCGGGATTGAGTTGAAGATTTCTTCTGAAGGGCTCCTGCTCAGTAATCCAAAAACCACAAATAGCAGAGGATTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

1190

Amino Acids

134.21

Weight (kDa)

6.15

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 167 - 326 3.6e-23 NB-ARC domain
LRR_14 PF23598 600 - 875 3e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 777 - 843 4e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 802 - 905 5.9e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 944 - 1058 2.2e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1064 - 1165 7.5e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1123
Acc16I TGCGCA 2 cut(s) 276, 1836
AclWI GGATC 2 cut(s) 1905, 2918
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 5 cut(s) 6, 251, 490, 1646, 3049
AcuI CTGAAG 5 cut(s) 1593, 1718, 2237, 2295, 3540
AcyI GRCGYC 2 cut(s) 61, 1120
AdeI CACNNNGTG 1 cut(s) 3296
AfaI GTAC 3 cut(s) 475, 1578, 2843
AfiI CCNNNNNNNGG 2 cut(s) 755, 3130
AflII CTTAAG 1 cut(s) 1208
AflIII ACRYGT 2 cut(s) 869, 1462
AhdI GACNNNNNGTC 1 cut(s) 285
AjnI CCWGG 2 cut(s) 1626, 2817
Alw21I GWGCWC 3 cut(s) 1075, 2163, 2502
Alw26I GTCTC 6 cut(s) 255, 1111, 1959, 2055, 2369, 3483
AlwI GGATC 2 cut(s) 1905, 2918
AlwNI CAGNNNCTG 2 cut(s) 2350, 3492
Ama87I CYCGRG 1 cut(s) 3439
AoxI GGCC 4 cut(s) 265, 1502, 1624, 3112
ApaI GGGCCC 1 cut(s) 3116
ApeKI GCWGC 4 cut(s) 14, 106, 1634, 3185
ApoI RAATTY 5 cut(s) 6, 251, 490, 1646, 3049
AseI ATTAAT 3 cut(s) 1257, 2397, 3018
Asp700I GAANNNNTTC 3 cut(s) 543, 594, 2340
AspLEI GCGC 3 cut(s) 277, 748, 1837
AspS9I GGNCC 4 cut(s) 2242, 2846, 3112, 3113
AsuC2I CCSGG 4 cut(s) 24, 463, 1441, 3388
AsuII TTCGAA 1 cut(s) 904
AvaI CYCGRG 1 cut(s) 3439
AvaII GGWCC 2 cut(s) 2242, 2846
BaeGI GKGCMC 2 cut(s) 2908, 3116
BanII GRGCYC 4 cut(s) 1075, 2502, 3116, 3528
BauI CACGAG 1 cut(s) 1329
BbsI GAAGAC 4 cut(s) 221, 281, 581, 2186
Bbv12I GWGCWC 3 cut(s) 1075, 2163, 2502
BbvI GCAGC 3 cut(s) 93, 1621, 3197
BceAI ACGGC 2 cut(s) 280, 1198
BcgI CGANNNNNNTGC 2 cut(s) 1862, 1896
BciT130I CCWGG 2 cut(s) 1628, 2819
BciVI GTATCC 1 cut(s) 2527
BclI TGATCA 5 cut(s) 163, 364, 1186, 2449, 3349
BcnI CCSGG 4 cut(s) 24, 463, 1441, 3388
BcoDI GTCTC 6 cut(s) 255, 1111, 1959, 2055, 2369, 3483
BfaI CTAG 7 cut(s) 77, 734, 755, 1598, 1796, 1961, 2025
BfmI CTRYAG 3 cut(s) 107, 1701, 3261
BfoI RGCGCY 1 cut(s) 749
BfrI CTTAAG 1 cut(s) 1208
BfuI GTATCC 1 cut(s) 2527
BisI GCNGC 7 cut(s) 15, 107, 268, 1635, 2724, 2925, 3186
BlsI GCNGC 7 cut(s) 16, 108, 269, 1636, 2725, 2926, 3187
Bme1390I CCNGG 6 cut(s) 24, 463, 1441, 1628, 2819, 3388
Bme18I GGWCC 2 cut(s) 2242, 2846
BmeRI GACNNNNNGTC 1 cut(s) 285
BmeT110I CYCGRG 1 cut(s) 3439
BmgT120I GGNCC 4 cut(s) 2242, 2846, 3112, 3113
BmiI GGNNCC 2 cut(s) 3114, 3527
BmrFI CCNGG 6 cut(s) 24, 463, 1441, 1628, 2819, 3388
BmrI ACTGGG 2 cut(s) 2911, 3302
BmsI GCATC 5 cut(s) 217, 1324, 1457, 1646, 2074
BmuI ACTGGG 2 cut(s) 2911, 3302
BpiI GAAGAC 4 cut(s) 221, 281, 581, 2186
BpmI CTGGAG 2 cut(s) 1193, 2098
Bpu10I CCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 904
BpuEI CTTGAG 4 cut(s) 1135, 2452, 2960, 2978
BpuMI CCSGG 4 cut(s) 24, 463, 1441, 3388
Bsa29I ATCGAT 1 cut(s) 2580
BsaHI GRCGYC 2 cut(s) 61, 1120
BsaJI CCNNGG 5 cut(s) 1580, 1627, 2245, 2817, 2991
BsaXI ACNNNNNCTCC 2 cut(s) 2096, 2126
Bsc4I CCNNNNNNNGG 2 cut(s) 755, 3130
Bse118I RCCGGY 1 cut(s) 1045
Bse1I ACTGG 6 cut(s) 215, 828, 2115, 2619, 2906, 3297
Bse3DI GCAATG 2 cut(s) 1980, 2155
BseBI CCWGG 2 cut(s) 1628, 2819
BseCI ATCGAT 1 cut(s) 2580
BseDI CCNNGG 5 cut(s) 1580, 1627, 2245, 2817, 2991
BseLI CCNNNNNNNGG 2 cut(s) 755, 3130
BseMI GCAATG 2 cut(s) 1980, 2155
BseMII CTCAG 5 cut(s) 248, 512, 1892, 3308, 3547
BseNI ACTGG 6 cut(s) 215, 828, 2115, 2619, 2906, 3297
BseRI GAGGAG 4 cut(s) 192, 371, 2975, 3044
BseSI GKGCMC 2 cut(s) 2908, 3116
BseX3I CGGCCG 1 cut(s) 265
BseXI GCAGC 3 cut(s) 93, 1621, 3197
BseYI CCCAGC 3 cut(s) 2228, 2857, 2976
BsgI GTGCAG 1 cut(s) 1549
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 4 cut(s) 267, 1504, 1626, 3114
BshVI ATCGAT 1 cut(s) 2580
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 3 cut(s) 1075, 2163, 2502
BsiHKCI CYCGRG 1 cut(s) 3439
BsiSI CCGG 5 cut(s) 24, 462, 1046, 1441, 3388
BslI CCNNNNNNNGG 2 cut(s) 755, 3130
BsmAI GTCTC 6 cut(s) 255, 1111, 1959, 2055, 2369, 3483
BsmBI CGTCTC 3 cut(s) 255, 1111, 2369
BsmI GAATGC 1 cut(s) 1416
BsnI GGCC 4 cut(s) 267, 1504, 1626, 3114
BsoBI CYCGRG 1 cut(s) 3439
Bsp119I TTCGAA 1 cut(s) 904
Bsp120I GGGCCC 1 cut(s) 3112
Bsp1286I GDGCHC 6 cut(s) 1075, 2163, 2502, 2908, 3116, 3528
BspANI GGCC 4 cut(s) 267, 1504, 1626, 3114
BspCNI CTCAG 5 cut(s) 249, 513, 1893, 3309, 3546
BspDI ATCGAT 1 cut(s) 2580
BspHI TCATGA 1 cut(s) 3352
BspLI GGNNCC 2 cut(s) 3114, 3527
BspMAI CTGCAG 2 cut(s) 111, 1705
BspPI GGATC 2 cut(s) 1905, 2918
BspT104I TTCGAA 1 cut(s) 904
BspTI CTTAAG 1 cut(s) 1208
BsrDI GCAATG 2 cut(s) 1980, 2155
BsrFI RCCGGY 1 cut(s) 1045
BsrI ACTGG 6 cut(s) 215, 828, 2115, 2619, 2906, 3297
BssAI RCCGGY 1 cut(s) 1045
BssECI CCNNGG 5 cut(s) 1580, 1627, 2245, 2817, 2991
BssNI GRCGYC 2 cut(s) 61, 1120
BssSI CACGAG 1 cut(s) 1329
BssT1I CCWWGG 1 cut(s) 1580
Bst2BI CACGAG 1 cut(s) 1329
Bst2UI CCWGG 2 cut(s) 1628, 2819
Bst4CI ACNGT 8 cut(s) 46, 202, 473, 1063, 1388, 2249, 2846, 3475
Bst6I CTCTTC 2 cut(s) 1737, 2532
BstACI GRCGYC 2 cut(s) 61, 1120
BstAFI CTTAAG 1 cut(s) 1208
BstAPI GCANNNNNTGC 1 cut(s) 1334
BstBI TTCGAA 1 cut(s) 904
BstC8I GCNNGC 6 cut(s) 606, 2223, 2922, 3068, 3112, 3337
BstDSI CCRYGG 2 cut(s) 2245, 2991
BstH2I RGCGCY 1 cut(s) 749
BstHHI GCGC 3 cut(s) 277, 748, 1837
BstMAI GTCTC 6 cut(s) 255, 1111, 1959, 2055, 2369, 3483
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 4 cut(s) 743, 1166, 1334, 2442
BstNI CCWGG 2 cut(s) 1628, 2819
BstNSI RCATGY 5 cut(s) 873, 1406, 1466, 3041, 3070
BstSCI CCNGG 6 cut(s) 22, 461, 1439, 1626, 2817, 3386
BstSFI CTRYAG 3 cut(s) 107, 1701, 3261
BstSLI GKGCMC 2 cut(s) 2908, 3116
BstV1I GCAGC 3 cut(s) 93, 1621, 3197
BstV2I GAAGAC 4 cut(s) 221, 281, 581, 2186
BstX2I RGATCY 2 cut(s) 1897, 2910
BstXI CCANNNNNNTGG 2 cut(s) 703, 2122
BstYI RGATCY 2 cut(s) 1897, 2910
BstZI CGGCCG 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 2580
BsuI GTATCC 1 cut(s) 2527
BsuRI GGCC 4 cut(s) 267, 1504, 1626, 3114
BsuTUI ATCGAT 1 cut(s) 2580
BtgI CCRYGG 2 cut(s) 2245, 2991
BtgZI GCGATG 1 cut(s) 2247
BtsI GCAGTG 1 cut(s) 1710
BtsIMutI CAGTG 4 cut(s) 1393, 1710, 3290, 3480
Cac8I GCNNGC 6 cut(s) 606, 2223, 2922, 3068, 3112, 3337
CaiI CAGNNNCTG 2 cut(s) 2350, 3492
CciI TCATGA 1 cut(s) 3352
CfoI GCGC 3 cut(s) 277, 748, 1837
Cfr10I RCCGGY 1 cut(s) 1045
Cfr13I GGNCC 4 cut(s) 2242, 2846, 3112, 3113
ClaI ATCGAT 1 cut(s) 2580
CseI GACGC 3 cut(s) 69, 2309, 3421
Csp6I GTAC 3 cut(s) 474, 1577, 2842
CspCI CAANNNNNGTGG 4 cut(s) 568, 603, 1015, 1050
CviQI GTAC 3 cut(s) 474, 1577, 2842
DraI TTTAAA 2 cut(s) 936, 1545
DraIII CACNNNGTG 1 cut(s) 3296
DriI GACNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 265
EagI CGGCCG 1 cut(s) 265
Eam1104I CTCTTC 2 cut(s) 1737, 2532
Eam1105I GACNNNNNGTC 1 cut(s) 285
EarI CTCTTC 2 cut(s) 1737, 2532
EciI GGCGGA 1 cut(s) 368
Ecl136II GAGCTC 2 cut(s) 1073, 2500
EclXI CGGCCG 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 1580
Eco147I AGGCCT 1 cut(s) 1626
Eco24I GRGCYC 4 cut(s) 1075, 2502, 3116, 3528
Eco32I GATATC 3 cut(s) 1393, 2168, 2584
Eco47I GGWCC 2 cut(s) 2242, 2846
Eco52I CGGCCG 1 cut(s) 265
Eco53kI GAGCTC 2 cut(s) 1073, 2500
Eco57I CTGAAG 5 cut(s) 1593, 1718, 2237, 2295, 3540
Eco88I CYCGRG 1 cut(s) 3439
EcoICRI GAGCTC 2 cut(s) 1073, 2500
EcoRI GAATTC 3 cut(s) 251, 490, 1646
EcoRII CCWGG 2 cut(s) 1626, 2817
EcoRV GATATC 3 cut(s) 1393, 2168, 2584
EcoT14I CCWWGG 1 cut(s) 1580
EcoT22I ATGCAT 3 cut(s) 1339, 1404, 1416
EcoT38I GRGCYC 4 cut(s) 1075, 2502, 3116, 3528
ErhI CCWWGG 1 cut(s) 1580
Esp3I CGTCTC 3 cut(s) 255, 1111, 2369
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FauI CCCGC 3 cut(s) 2821, 3103, 3488
FauNDI CATATG 2 cut(s) 424, 2438
FbaI TGATCA 5 cut(s) 163, 364, 1186, 2449, 3349
Fnu4HI GCNGC 7 cut(s) 15, 107, 268, 1635, 2724, 2925, 3186
FriOI GRGCYC 4 cut(s) 1075, 2502, 3116, 3528
Fsp4HI GCNGC 7 cut(s) 15, 107, 268, 1635, 2724, 2925, 3186
FspBI CTAG 7 cut(s) 77, 734, 755, 1598, 1796, 1961, 2025
FspI TGCGCA 2 cut(s) 276, 1836
GlaI GCGC 3 cut(s) 276, 747, 1836
GluI GCNGC 7 cut(s) 15, 107, 268, 1635, 2724, 2925, 3186
GsaI CCCAGC 3 cut(s) 2232, 2861, 2980
GsuI CTGGAG 2 cut(s) 1193, 2098
HaeII RGCGCY 1 cut(s) 749
HaeIII GGCC 4 cut(s) 267, 1504, 1626, 3114
HapII CCGG 5 cut(s) 24, 462, 1046, 1441, 3388
HgaI GACGC 3 cut(s) 69, 2309, 3421
HhaI GCGC 3 cut(s) 277, 748, 1837
Hin1I GRCGYC 2 cut(s) 61, 1120
Hin6I GCGC 3 cut(s) 275, 746, 1835
HinP1I GCGC 3 cut(s) 275, 746, 1835
HincII GTYRAC 2 cut(s) 619, 867
HindII GTYRAC 2 cut(s) 619, 867
HindIII AAGCTT 1 cut(s) 1358
HpaI GTTAAC 2 cut(s) 619, 867
HpaII CCGG 5 cut(s) 24, 462, 1046, 1441, 3388
Hpy166II GTNNAC 5 cut(s) 42, 619, 850, 867, 1490
Hpy8I GTNNAC 5 cut(s) 42, 619, 850, 867, 1490
HpyAV CCTTC 6 cut(s) 761, 1662, 1734, 2398, 3205, 3515
HpyCH4III ACNGT 8 cut(s) 46, 202, 473, 1063, 1388, 2249, 2846, 3475
HpyCH4IV ACGT 3 cut(s) 1120, 1983, 2731
HpyF10VI GCNNNNNNNGC 4 cut(s) 743, 1166, 1334, 2442
HpySE526I ACGT 3 cut(s) 1120, 1983, 2731
Hsp92I GRCGYC 2 cut(s) 61, 1120
HspAI GCGC 3 cut(s) 275, 746, 1835
Ksp22I TGATCA 5 cut(s) 163, 364, 1186, 2449, 3349
KspAI GTTAAC 2 cut(s) 619, 867
LmnI GCTCC 5 cut(s) 2158, 2230, 2497, 3362, 3531
Lsp1109I GCAGC 3 cut(s) 93, 1621, 3197
LweI GCATC 5 cut(s) 217, 1324, 1457, 1646, 2074
MaeI CTAG 7 cut(s) 77, 734, 755, 1598, 1796, 1961, 2025
MaeII ACGT 3 cut(s) 1120, 1983, 2731
MaeIII GTNAC 5 cut(s) 1057, 1342, 1716, 2673, 3409
MfeI CAATTG 4 cut(s) 54, 1014, 1190, 1874
MflI RGATCY 2 cut(s) 1897, 2910
MhlI GDGCHC 6 cut(s) 1075, 2163, 2502, 2908, 3116, 3528
MlyI GAGTC 4 cut(s) 516, 775, 3185, 3453
Mph1103I ATGCAT 3 cut(s) 1339, 1404, 1416
MroXI GAANNNNTTC 3 cut(s) 543, 594, 2340
MslI CAYNNNNRTG 1 cut(s) 3065
MspA1I CMGCKG 1 cut(s) 3110
MspCI CTTAAG 1 cut(s) 1208
MspI CCGG 5 cut(s) 24, 462, 1046, 1441, 3388
MspR9I CCNGG 6 cut(s) 24, 463, 1441, 1628, 2819, 3388
MunI CAATTG 4 cut(s) 54, 1014, 1190, 1874
Mva1269I GAATGC 1 cut(s) 1416
MvaI CCWGG 2 cut(s) 1628, 2819
MwoI GCNNNNNNNGC 4 cut(s) 743, 1166, 1334, 2442
NciI CCSGG 4 cut(s) 24, 463, 1441, 3388
NdeI CATATG 2 cut(s) 424, 2438
NlaIV GGNNCC 2 cut(s) 3114, 3527
NmeAIII GCCGAG 2 cut(s) 61, 1145
NmuCI GTSAC 2 cut(s) 1716, 3409
NsbI TGCGCA 2 cut(s) 276, 1836
NsiI ATGCAT 3 cut(s) 1339, 1404, 1416
NspI RCATGY 5 cut(s) 873, 1406, 1466, 3041, 3070
NspV TTCGAA 1 cut(s) 904
PaeI GCATGC 1 cut(s) 3070
PagI TCATGA 1 cut(s) 3352
PceI AGGCCT 1 cut(s) 1626
PciI ACATGT 2 cut(s) 869, 1462
PcsI WCGNNNNNNNCGW 1 cut(s) 2229
PctI GAATGC 1 cut(s) 1416
PdmI GAANNNNTTC 3 cut(s) 543, 594, 2340
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 7 cut(s) 16, 108, 269, 1636, 2725, 2926, 3187
PleI GAGTC 4 cut(s) 515, 775, 3184, 3453
PpsI GAGTC 4 cut(s) 515, 775, 3184, 3453
PscI ACATGT 2 cut(s) 869, 1462
PshBI ATTAAT 3 cut(s) 1257, 2397, 3018
Psp124BI GAGCTC 2 cut(s) 1075, 2502
Psp6I CCWGG 2 cut(s) 1626, 2817
PspFI CCCAGC 3 cut(s) 2228, 2857, 2976
PspGI CCWGG 2 cut(s) 1626, 2817
PspN4I GGNNCC 2 cut(s) 3114, 3527
PspOMI GGGCCC 1 cut(s) 3112
PspPI GGNCC 4 cut(s) 2242, 2846, 3112, 3113
PstI CTGCAG 2 cut(s) 111, 1705
PstNI CAGNNNCTG 2 cut(s) 2350, 3492
PsuI RGATCY 2 cut(s) 1897, 2910
RsaI GTAC 3 cut(s) 475, 1578, 2843
RsaNI GTAC 3 cut(s) 474, 1577, 2842
RseI CAYNNNNRTG 1 cut(s) 3065
SacI GAGCTC 2 cut(s) 1075, 2502
SatI GCNGC 7 cut(s) 15, 107, 268, 1635, 2724, 2925, 3186
Sau96I GGNCC 4 cut(s) 2242, 2846, 3112, 3113
SchI GAGTC 4 cut(s) 516, 775, 3185, 3453
ScrFI CCNGG 6 cut(s) 24, 463, 1441, 1628, 2819, 3388
SduI GDGCHC 6 cut(s) 1075, 2163, 2502, 2908, 3116, 3528
SfaNI GCATC 5 cut(s) 217, 1324, 1457, 1646, 2074
SfcI CTRYAG 3 cut(s) 107, 1701, 3261
SfuI TTCGAA 1 cut(s) 904
SinI GGWCC 2 cut(s) 2242, 2846
SmiMI CAYNNNNRTG 1 cut(s) 3065
SmlI CTYRAG 5 cut(s) 1114, 1208, 2431, 2939, 2957
SmoI CTYRAG 5 cut(s) 1114, 1208, 2431, 2939, 2957
SphI GCATGC 1 cut(s) 3070
SseBI AGGCCT 1 cut(s) 1626
SspI AATATT 2 cut(s) 2092, 3000
SspMI CTAG 7 cut(s) 77, 734, 755, 1598, 1796, 1961, 2025
SstI GAGCTC 2 cut(s) 1075, 2502
StuI AGGCCT 1 cut(s) 1626
StyD4I CCNGG 6 cut(s) 22, 461, 1439, 1626, 2817, 3386
StyI CCWWGG 1 cut(s) 1580
TaaI ACNGT 8 cut(s) 46, 202, 473, 1063, 1388, 2249, 2846, 3475
TaiI ACGT 3 cut(s) 1123, 1986, 2734
TaqI TCGA 7 cut(s) 192, 325, 480, 904, 1311, 1882, 2580
TaqII GACCGA 1 cut(s) 296
TatI WGTACW 1 cut(s) 473
TauI GCSGC 3 cut(s) 270, 2726, 2927
TscAI CASTG 4 cut(s) 1393, 1710, 3297, 3480
TseFI GTSAC 2 cut(s) 1716, 3409
TseI GCWGC 4 cut(s) 14, 106, 1634, 3185
Tsp45I GTSAC 2 cut(s) 1716, 3409
TspGWI ACGGA 6 cut(s) 1098, 1166, 2351, 2661, 2923, 3008
TspRI CASTG 4 cut(s) 1393, 1710, 3297, 3480
Vha464I CTTAAG 1 cut(s) 1208
VpaK11BI GGWCC 2 cut(s) 2242, 2846
VspI ATTAAT 3 cut(s) 1257, 2397, 3018
XapI RAATTY 5 cut(s) 6, 251, 490, 1646, 3049
XceI RCATGY 5 cut(s) 873, 1406, 1466, 3041, 3070
XcmI CCANNNNNNNNNTGG 2 cut(s) 2141, 2236
XmnI GAANNNNTTC 3 cut(s) 543, 594, 2340
XspI CTAG 7 cut(s) 77, 734, 755, 1598, 1796, 1961, 2025
ZraI GACGTC 1 cut(s) 1121
Zsp2I ATGCAT 3 cut(s) 1339, 1404, 1416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.