Rh5AG329000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003076
Physical Location & Seq
Forward (+)
50109458 .. 50148402
38945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG329000.1

Sequence Viewer

Length: 453 bp
ATGGGCACCCACGAACTTCAGAGGGAAGGGTATCAAGAAGAATCGGTCTATGATCAGAACCGATTTTGGGAAGGTGGAAAATCTAGGTACGATCCTGGACAGCGCACCATTCCACATTGGCCATTGCTCTATCCAAACGCTCCTTTATGTAAACTCTGCCATTTCTCATTGCAAACCATCCTCTATCCAAATCGAGCTTCATATTCAACCGACCTCGGCGGGGGAATTGAAATCAAGAACAGTAAGAATTGGGTAAACCTCTATGGGCTTGGGTTCGTTTTGGAAGAGATGTGTGCCAAGATTGTGATTGAGTTTAGGAGACGGGCTTTGGGAGCAAAAGAGAAAGGTGCTTTCGTTCTCGCAGCAAAAACGAATGTGCCAGTGGTGCCTATTACTCAAAGTAACATAATCCAAAAGTGGAGAAAGGTTAAGACTTGGAGAGGGAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

17.19

Weight (kDa)

9.54

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 5, 385
AciI CCGC 1 cut(s) 219
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 119
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 67, 220
AgsI TTSAA 2 cut(s) 207, 230
AjnI CCWGG 1 cut(s) 94
AjuI GAANNNNNNNTTGG 2 cut(s) 181, 213
AluBI AGCT 1 cut(s) 197
AluI AGCT 1 cut(s) 197
Alw26I GTCTC 1 cut(s) 313
AlwI GGATC 1 cut(s) 86
AoxI GGCC 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 105
BaeGI GKGCMC 1 cut(s) 8
BalI TGGCCA 1 cut(s) 121
BanI GGYRCC 2 cut(s) 5, 385
BbvI GCAGC 1 cut(s) 374
BccI CCATC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 96
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 313
BfaI CTAG 1 cut(s) 84
BisI GCNGC 1 cut(s) 363
BlsI GCNGC 1 cut(s) 364
Bme1390I CCNGG 1 cut(s) 96
BmiI GGNNCC 2 cut(s) 7, 387
BmrFI CCNGG 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 214
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 220
Bse1I ACTGG 1 cut(s) 380
Bse3DI GCAATG 2 cut(s) 122, 167
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 67, 220
BseMI GCAATG 2 cut(s) 122, 167
BseNI ACTGG 1 cut(s) 380
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 374
BshFI GGCC 1 cut(s) 121
BshNI GGYRCC 2 cut(s) 5, 385
BslI CCNNNNNNNGG 2 cut(s) 67, 220
BsmAI GTCTC 1 cut(s) 313
BsmBI CGTCTC 1 cut(s) 313
BsnI GGCC 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 2 cut(s) 52, 91
BspACI CCGC 1 cut(s) 219
BspANI GGCC 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 7, 387
BspPI GGATC 1 cut(s) 86
BspT107I GGYRCC 2 cut(s) 5, 385
BsrDI GCAATG 2 cut(s) 122, 167
BsrI ACTGG 1 cut(s) 380
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 2 cut(s) 52, 91
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 279
BstF5I GGATG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 2 cut(s) 55, 94
BstMAI GTCTC 1 cut(s) 313
BstMBI GATC 2 cut(s) 52, 91
BstMWI GCNNNNNNNGC 2 cut(s) 332, 385
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 121
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 387
CfoI GCGC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 88
CviJI RGCY 4 cut(s) 121, 197, 268, 326
CviKI_1 RGCY 4 cut(s) 121, 197, 268, 326
CviQI GTAC 1 cut(s) 88
DpnI GATC 2 cut(s) 54, 93
DpnII GATC 2 cut(s) 52, 91
EaeI YGGCCR 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 313
FaiI YATR 5 cut(s) 51, 148, 202, 264, 407
FauI CCCGC 1 cut(s) 212
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 1 cut(s) 363
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 1 cut(s) 363
FspBI CTAG 1 cut(s) 84
GlaI GCGC 1 cut(s) 104
GluI GCNGC 1 cut(s) 363
HaeIII GGCC 1 cut(s) 121
HhaI GCGC 1 cut(s) 105
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 152, 256
Hpy188I TCNGA 2 cut(s) 21, 57
Hpy188III TCNNGA 2 cut(s) 35, 235
Hpy8I GTNNAC 2 cut(s) 152, 256
HpyAV CCTTC 2 cut(s) 20, 65
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4V TGCA 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 2 cut(s) 332, 385
HspAI GCGC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 2 cut(s) 52, 91
LmnI GCTCC 2 cut(s) 145, 332
LpnPI CCDG 3 cut(s) 81, 108, 393
Lsp1109I GCAGC 1 cut(s) 374
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 401
MalI GATC 2 cut(s) 54, 93
MboI GATC 2 cut(s) 52, 91
MboII GAAGA 2 cut(s) 50, 296
MhlI GDGCHC 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 121
MluCI AATT 2 cut(s) 225, 247
MluNI TGGCCA 1 cut(s) 121
MnlI CCTC 5 cut(s) 15, 191, 224, 269, 434
Mox20I TGGCCA 1 cut(s) 121
MscI TGGCCA 1 cut(s) 121
MseI TTAA 2 cut(s) 429, 451
Msp20I TGGCCA 1 cut(s) 121
MspR9I CCNGG 1 cut(s) 96
MvaI CCWGG 1 cut(s) 96
MwoI GCNNNNNNNGC 2 cut(s) 332, 385
NdeII GATC 2 cut(s) 52, 91
NlaIV GGNNCC 2 cut(s) 7, 387
NmeAIII GCCGAG 1 cut(s) 195
PfeI GAWTC 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 2 cut(s) 7, 387
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SaqAI TTAA 2 cut(s) 429, 451
SatI GCNGC 1 cut(s) 363
Sau3AI GATC 2 cut(s) 52, 91
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 1 cut(s) 8
SetI ASST 7 cut(s) 76, 89, 199, 216, 261, 349, 429
Sse9I AATT 2 cut(s) 225, 247
SsiI CCGC 1 cut(s) 219
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 193
TaqII GACCGA 1 cut(s) 34
TasI AATT 2 cut(s) 225, 247
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 2 cut(s) 429, 451
Tru9I TTAA 2 cut(s) 429, 451
TscAI CASTG 1 cut(s) 387
TseI GCWGC 1 cut(s) 362
TspDTI ATGAA 1 cut(s) 189
TspRI CASTG 1 cut(s) 387
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.