Rh5BG396600

Formin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003076
Physical Location & Seq
Forward (+)
64137878 .. 64148363
10486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG396600.1

Sequence Viewer

Length: 672 bp
ATGCCAGAGTTGCTGGATTTTGATAAGGATCTTATTCATTTGGAAGCTGCCTCTAAGATTCCATTGAAAGCTTTAGCTGAAGAAATGCAAGCCGTGAGTAAAGGTCCTGAAAAGGTTAAGCAAGAACTTGCTGCTGCTGAAAACGACGGTGTAATCTCTTCTGGTTTTCGAAAGAACGAGGGAATCCAAGGATCTTTTCTAGAAGCATACTACACCACAATAAGGACTGGAGAAACACTCGGAGATGGAGAGATTAGCACAGATTGCTTCACCGTAGCAGACTTCAATGCTCCTATCAATTCTGCAGATTCCATAACCGGAACCTCCAATAGTCCAACCTCCATCCCCGCCGTAGGACTTGGATCAACCACCAGTCGCCGCCGTTTCACCGCCGTTCCACCGCCCAATCCTTCATCCCCATCAATACCCTCGATATCCATTGCATTCTCATCTAGTGTTCCCCTTCTCCCAGTTTCAGATTCCCACTCGTCTCGTGAGCGGATTAAGCCAATCGTTTGGAATTCAGGTGCCGTCATAGTCCACCCAGAAGGTTTTGCTCGGTGTAACCCAAATCTGGTACCTCCACTCCCAGATCGAGCAGCTTCCTCGAGAGCCCTCTCCTGCACTGCCAGCCAGGCTTCCTTCCGTTCGGCATTGAGAAGCACATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FH2 PTEN_C2

Protein Analysis

223

Amino Acids

23.5

Weight (kDa)

5.66

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FH2 PF02181 1 - 58 1.3e-06 Formin Homology 2 Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 577
AccB1I GGYRCC 2 cut(s) 527, 577
AccBSI CCGCTC 1 cut(s) 499
AciI CCGC 5 cut(s) 348, 379, 390, 401, 499
AclWI GGATC 3 cut(s) 36, 199, 370
AcsI RAATTY 1 cut(s) 520
AcuI CTGAAG 1 cut(s) 99
AfaI GTAC 1 cut(s) 579
AfiI CCNNNNNNNGG 3 cut(s) 222, 353, 574
AgsI TTSAA 2 cut(s) 67, 286
AjnI CCWGG 1 cut(s) 633
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 4 cut(s) 47, 71, 77, 602
AluI AGCT 4 cut(s) 47, 71, 77, 602
Alw26I GTCTC 1 cut(s) 495
AlwI GGATC 3 cut(s) 36, 199, 370
Ama87I CYCGRG 1 cut(s) 607
ApeKI GCWGC 4 cut(s) 47, 131, 134, 599
ApoI RAATTY 1 cut(s) 520
Asp718I GGTACC 1 cut(s) 577
AspS9I GGNCC 1 cut(s) 104
AsuHPI GGTGA 2 cut(s) 262, 379
AsuII TTCGAA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 1 cut(s) 104
BanI GGYRCC 2 cut(s) 527, 577
BanII GRGCYC 1 cut(s) 616
BauI CACGAG 1 cut(s) 492
BbvI GCAGC 4 cut(s) 34, 118, 121, 611
BccI CCATC 3 cut(s) 239, 350, 427
BceAI ACGGC 5 cut(s) 77, 335, 366, 377, 515
BcgI CGANNNNNNTGC 2 cut(s) 588, 622
BciT130I CCWGG 1 cut(s) 635
BcoDI GTCTC 1 cut(s) 495
BfaI CTAG 2 cut(s) 200, 453
BfmI CTRYAG 1 cut(s) 303
BglI GCCNNNNNGGC 1 cut(s) 635
BisI GCNGC 5 cut(s) 48, 132, 135, 379, 600
BlsI GCNGC 5 cut(s) 49, 133, 136, 380, 601
Bme1390I CCNGG 1 cut(s) 635
Bme18I GGWCC 1 cut(s) 104
BmeT110I CYCGRG 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 3 cut(s) 322, 529, 579
BmrFI CCNGG 1 cut(s) 635
BmrI ACTGGG 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 464
BplI GAGNNNNNCTC 2 cut(s) 222, 254
BpmI CTGGAG 1 cut(s) 249
Bpu14I TTCGAA 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 187
BsaWI WCCGGW 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 353, 574
Bse1I ACTGG 3 cut(s) 232, 372, 470
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 1 cut(s) 27
BseBI CCWGG 1 cut(s) 635
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 2 cut(s) 342, 413
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 3 cut(s) 222, 353, 574
BseMI GCAATG 1 cut(s) 438
BseNI ACTGG 3 cut(s) 232, 372, 470
BseXI GCAGC 4 cut(s) 34, 118, 121, 611
BsgI GTGCAG 1 cut(s) 607
BshNI GGYRCC 2 cut(s) 527, 577
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 1 cut(s) 318
BslI CCNNNNNNNGG 3 cut(s) 222, 353, 574
BsmAI GTCTC 1 cut(s) 495
BsmBI CGTCTC 1 cut(s) 495
BsmI GAATGC 1 cut(s) 443
BsoBI CYCGRG 1 cut(s) 607
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 4 cut(s) 28, 191, 362, 592
BspACI CCGC 5 cut(s) 348, 379, 390, 401, 499
BspLI GGNNCC 3 cut(s) 322, 529, 579
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 3 cut(s) 36, 199, 370
BspT104I TTCGAA 1 cut(s) 169
BspT107I GGYRCC 2 cut(s) 527, 577
BsrBI CCGCTC 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 3 cut(s) 232, 372, 470
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 4 cut(s) 28, 191, 362, 592
BssSI CACGAG 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 187
Bst2BI CACGAG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 635
Bst4CI ACNGT 2 cut(s) 149, 274
Bst6I CTCTTC 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 90, 631
BstDEI CTNAG 1 cut(s) 54
BstF5I GGATG 2 cut(s) 342, 413
BstKTI GATC 4 cut(s) 31, 194, 365, 595
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 4 cut(s) 28, 191, 362, 592
BstMWI GCNNNNNNNGC 5 cut(s) 10, 264, 505, 630, 635
BstNI CCWGG 1 cut(s) 635
BstSCI CCNGG 1 cut(s) 633
BstSFI CTRYAG 1 cut(s) 303
BstV1I GCAGC 4 cut(s) 34, 118, 121, 611
BstX2I RGATCY 2 cut(s) 28, 191
BstXI CCANNNNNNTGG 1 cut(s) 516
BstYI RGATCY 2 cut(s) 28, 191
BtsCI GGATG 2 cut(s) 342, 413
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 1 cut(s) 624
Cac8I GCNNGC 2 cut(s) 90, 631
Cfr13I GGNCC 1 cut(s) 104
Csp6I GTAC 1 cut(s) 578
CviJI RGCY 9 cut(s) 47, 71, 77, 92, 508, 602, 614, 633, 638
CviKI_1 RGCY 9 cut(s) 47, 71, 77, 92, 508, 602, 614, 633, 638
CviQI GTAC 1 cut(s) 578
DdeI CTNAG 1 cut(s) 54
DpnI GATC 4 cut(s) 30, 193, 364, 594
DpnII GATC 4 cut(s) 28, 191, 362, 592
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 616
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 607
EcoO109I RGGNCCY 1 cut(s) 104
EcoRI GAATTC 1 cut(s) 520
EcoRII CCWGG 1 cut(s) 633
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 187
Esp3I CGTCTC 1 cut(s) 495
FaiI YATR 4 cut(s) 208, 314, 536, 670
FauI CCCGC 1 cut(s) 355
Fnu4HI GCNGC 5 cut(s) 48, 132, 135, 379, 600
FokI GGATG 2 cut(s) 329, 400
FriOI GRGCYC 1 cut(s) 616
Fsp4HI GCNGC 5 cut(s) 48, 132, 135, 379, 600
FspBI CTAG 2 cut(s) 200, 453
GluI GCNGC 5 cut(s) 48, 132, 135, 379, 600
GsuI CTGGAG 1 cut(s) 249
HapII CCGG 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 4 cut(s) 58, 183, 308, 479
HpaII CCGG 1 cut(s) 318
HphI GGTGA 2 cut(s) 262, 379
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 2 cut(s) 242, 478
Hpy188III TCNNGA 4 cut(s) 107, 200, 494, 609
Hpy8I GTNNAC 1 cut(s) 541
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 4 cut(s) 420, 473, 542, 652
HpyCH4III ACNGT 2 cut(s) 149, 274
HpyCH4V TGCA 4 cut(s) 88, 305, 443, 624
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 264, 505, 630, 635
HpyF3I CTNAG 1 cut(s) 54
KpnI GGTACC 1 cut(s) 581
Kzo9I GATC 4 cut(s) 28, 191, 362, 592
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 4 cut(s) 34, 118, 121, 611
MaeI CTAG 2 cut(s) 200, 453
MaeIII GTNAC 1 cut(s) 563
MalI GATC 4 cut(s) 30, 193, 364, 594
MbiI CCGCTC 1 cut(s) 499
MboI GATC 4 cut(s) 28, 191, 362, 592
MboII GAAGA 2 cut(s) 92, 150
MflI RGATCY 2 cut(s) 28, 191
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 2 cut(s) 298, 520
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 8 cut(s) 61, 172, 334, 349, 439, 591, 616, 626
MseI TTAA 2 cut(s) 117, 504
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 1 cut(s) 635
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 1 cut(s) 635
MwoI GCNNNNNNNGC 5 cut(s) 10, 264, 505, 630, 635
NdeII GATC 4 cut(s) 28, 191, 362, 592
NlaIV GGNNCC 3 cut(s) 322, 529, 579
NspV TTCGAA 1 cut(s) 169
PaeR7I CTCGAG 1 cut(s) 607
PctI GAATGC 1 cut(s) 443
PfeI GAWTC 4 cut(s) 58, 183, 308, 479
PkrI GCNGC 5 cut(s) 49, 133, 136, 380, 601
PpuMI RGGWCCY 1 cut(s) 104
Psp5II RGGWCCY 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 633
PspGI CCWGG 1 cut(s) 633
PspN4I GGNNCC 3 cut(s) 322, 529, 579
PspPI GGNCC 1 cut(s) 104
PspPPI RGGWCCY 1 cut(s) 104
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 2 cut(s) 28, 191
RsaI GTAC 1 cut(s) 579
RsaNI GTAC 1 cut(s) 578
SaqAI TTAA 2 cut(s) 117, 504
SatI GCNGC 5 cut(s) 48, 132, 135, 379, 600
Sau3AI GATC 4 cut(s) 28, 191, 362, 592
Sau96I GGNCC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 635
SduI GDGCHC 1 cut(s) 616
SfcI CTRYAG 1 cut(s) 303
Sfr274I CTCGAG 1 cut(s) 607
SfuI TTCGAA 1 cut(s) 169
SinI GGWCC 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 607
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
Sse9I AATT 2 cut(s) 298, 520
SsiI CCGC 5 cut(s) 348, 379, 390, 401, 499
SspMI CTAG 2 cut(s) 200, 453
StyD4I CCNGG 1 cut(s) 633
StyI CCWWGG 1 cut(s) 187
TaaI ACNGT 2 cut(s) 149, 274
TaqI TCGA 4 cut(s) 169, 431, 595, 608
TasI AATT 2 cut(s) 298, 520
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 4 cut(s) 58, 183, 308, 479
Tru1I TTAA 2 cut(s) 117, 504
Tru9I TTAA 2 cut(s) 117, 504
TscAI CASTG 1 cut(s) 631
TseI GCWGC 4 cut(s) 47, 131, 134, 599
TspDTI ATGAA 2 cut(s) 26, 402
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 1 cut(s) 631
VpaK11BI GGWCC 1 cut(s) 104
XapI RAATTY 1 cut(s) 520
XbaI TCTAGA 1 cut(s) 199
XhoI CTCGAG 1 cut(s) 607
XspI CTAG 2 cut(s) 200, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.