RLG00000020042

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003076
Physical Location & Seq
Forward (+)
60471756 .. 60478418
6663 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020042

Sequence Viewer

Length: 747 bp
ATGCAATATAAAATGACATTGCATGTGCATATCAGCAAATTAATGTTGCCTGAGAAGTTCGGCTATAATTCTGGGTTCCTCTTCTTTTTGGAAGTATCTGTTTTCACTAATATCTCCACACCGACATCGGTTCTTGCAGACTGTCAAGAAGAAGAGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGGGAGACGGGAAGAAGTTGTTGCTCGATTCGAACAAATCATCCCAGGGGTATTGTTCCTGCTGCATTCAATTGCTTTGGGAGTCATTTCTGTTGAGAAGGGATGGAGCTATCCCTGATTCATGTATTTCTTATTATTCATGCCAATTATATAGGTCTGTTTTCTTCTTTGGAAGGGAGGAAGAAGCCGTGAAGAAATCAAGGCTGGTCTGCGCCGAAACATTGAAGCCTTTACAGACTGACCTACAAATCAAAGTGCGAGTCTGCAGGGTGTGGAGAGCGAAGAAATACAGACCTGTTCCTAGAAACAATGGTCTTCAGTTTGTTTTAGTTAATGAGAAGAAGGAGGCAATCCAAGGATCTTTTCTAGAAGCATACTACACCACAATAAGGACTGTGACAGGCAAGCAGAAAAACATGGAACTACGTCATCATCATCAGCACCATTCTGCAACTACAACTCCAATTGGAGCCATCACTTTTTTGAACCAACACCAGCATTTTACAAAGCTGCAACTGAAACAACCAACTGGAGTACTTTGTACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Helitron_like_N Herpes_Helicase PIF1 REPA_OB_2

Protein Analysis

249

Amino Acids

28.76

Weight (kDa)

6.16

Isoelectric Point (pI)

58.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 562
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 2 cut(s) 732, 739
AfiI CCNNNNNNNGG 1 cut(s) 585
AgsI TTSAA 3 cut(s) 266, 421, 682
AjnI CCWGG 1 cut(s) 240
AjuI GAANNNNNNNTTGG 2 cut(s) 543, 575
AloI GAACNNNNNNTCC 2 cut(s) 221, 253
AluBI AGCT 2 cut(s) 305, 706
AluI AGCT 2 cut(s) 305, 706
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 1 cut(s) 562
ApeKI GCWGC 2 cut(s) 258, 706
ArsI GACNNNNNNTTYG 2 cut(s) 441, 473
AseI ATTAAT 1 cut(s) 41
AspLEI GCGC 1 cut(s) 410
AsuII TTCGAA 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 245, 693
BccI CCATC 2 cut(s) 293, 677
BceAI ACGGC 1 cut(s) 368
BciT130I CCWGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 195
BfaI CTAG 2 cut(s) 498, 563
BfmI CTRYAG 1 cut(s) 460
BisI GCNGC 2 cut(s) 259, 707
BlsI GCNGC 2 cut(s) 260, 708
BmcAI AGTACT 1 cut(s) 732
Bme1390I CCNGG 1 cut(s) 242
BmiI GGNNCC 2 cut(s) 77, 667
BmrFI CCNGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 503
BpmI CTGGAG 1 cut(s) 747
Bpu14I TTCGAA 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 240, 241, 550
BsaXI ACNNNNNCTCC 2 cut(s) 640, 670
Bsc4I CCNNNNNNNGG 1 cut(s) 585
Bse1I ACTGG 1 cut(s) 730
Bse3DI GCAATG 1 cut(s) 17
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 3 cut(s) 240, 241, 550
BseGI GGATG 2 cut(s) 236, 304
BseLI CCNNNNNNNGG 1 cut(s) 585
BseMI GCAATG 1 cut(s) 17
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 1 cut(s) 730
BseRI GAGGAG 4 cut(s) 170, 173, 176, 179
BseXI GCAGC 2 cut(s) 245, 693
BslI CCNNNNNNNGG 1 cut(s) 585
BsmAI GTCTC 1 cut(s) 195
BsmBI CGTCTC 1 cut(s) 195
BsmI GAATGC 1 cut(s) 261
Bsp119I TTCGAA 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 737
Bsp143I GATC 1 cut(s) 554
BspCNI CTCAG 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 77, 667
BspMAI CTGCAG 1 cut(s) 464
BspPI GGATC 1 cut(s) 562
BspT104I TTCGAA 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 17
BsrGI TGTACA 1 cut(s) 737
BsrI ACTGG 1 cut(s) 730
BssECI CCNNGG 3 cut(s) 240, 241, 550
BssMI GATC 1 cut(s) 554
BssT1I CCWWGG 1 cut(s) 550
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 143, 592
Bst6I CTCTTC 3 cut(s) 86, 147, 189
BstAUI TGTACA 1 cut(s) 737
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 1 cut(s) 602
BstDEI CTNAG 1 cut(s) 51
BstF5I GGATG 2 cut(s) 236, 304
BstHHI GCGC 1 cut(s) 410
BstKTI GATC 1 cut(s) 557
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 1 cut(s) 554
BstNI CCWGG 1 cut(s) 242
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 240
BstSFI CTRYAG 1 cut(s) 460
BstV1I GCAGC 2 cut(s) 245, 693
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 554
BstYI RGATCY 1 cut(s) 554
BtsCI GGATG 2 cut(s) 236, 304
Cac8I GCNNGC 1 cut(s) 602
CfoI GCGC 1 cut(s) 410
Csp6I GTAC 2 cut(s) 731, 738
CviAII CATG 4 cut(s) 23, 318, 336, 613
CviJI RGCY 7 cut(s) 63, 305, 383, 400, 424, 668, 706
CviKI_1 RGCY 7 cut(s) 63, 305, 383, 400, 424, 668, 706
CviQI GTAC 2 cut(s) 731, 738
DdeI CTNAG 1 cut(s) 51
DpnI GATC 1 cut(s) 556
DpnII GATC 1 cut(s) 554
Eam1104I CTCTTC 3 cut(s) 86, 147, 189
EarI CTCTTC 3 cut(s) 86, 147, 189
Eco130I CCWWGG 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 550
ErhI CCWWGG 1 cut(s) 550
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 4 cut(s) 26, 321, 339, 616
FatI CATG 4 cut(s) 22, 317, 335, 612
Fnu4HI GCNGC 2 cut(s) 259, 707
FokI GGATG 2 cut(s) 223, 311
Fsp4HI GCNGC 2 cut(s) 259, 707
FspBI CTAG 2 cut(s) 498, 563
GlaI GCGC 1 cut(s) 409
GluI GCNGC 2 cut(s) 259, 707
GsuI CTGGAG 1 cut(s) 747
HhaI GCGC 1 cut(s) 410
Hin1II CATG 4 cut(s) 26, 321, 339, 616
Hin6I GCGC 1 cut(s) 408
HinP1I GCGC 1 cut(s) 408
HinfI GANTC 4 cut(s) 224, 278, 314, 456
Hpy188III TCNNGA 2 cut(s) 146, 563
HpyAV CCTTC 3 cut(s) 288, 363, 532
HpyCH4III ACNGT 2 cut(s) 143, 592
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 8 cut(s) 4, 22, 28, 137, 261, 462, 647, 709
HpyF3I CTNAG 1 cut(s) 51
HpySE526I ACGT 1 cut(s) 622
Hsp92II CATG 4 cut(s) 26, 321, 339, 616
HspAI GCGC 1 cut(s) 408
Kzo9I GATC 1 cut(s) 554
LmnI GCTCC 2 cut(s) 302, 665
Lsp1109I GCAGC 2 cut(s) 245, 693
MaeI CTAG 2 cut(s) 498, 563
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 1 cut(s) 592
MalI GATC 1 cut(s) 556
MboI GATC 1 cut(s) 554
MfeI CAATTG 2 cut(s) 266, 660
MflI RGATCY 1 cut(s) 554
MluCI AATT 5 cut(s) 38, 67, 266, 341, 660
MlyI GAGTC 2 cut(s) 287, 465
MnlI CCTC 9 cut(s) 89, 148, 151, 154, 157, 160, 190, 367, 535
MseI TTAA 2 cut(s) 41, 528
MspR9I CCNGG 1 cut(s) 242
MunI CAATTG 2 cut(s) 266, 660
Mva1269I GAATGC 1 cut(s) 261
MvaI CCWGG 1 cut(s) 242
NdeII GATC 1 cut(s) 554
NlaIII CATG 4 cut(s) 26, 321, 339, 616
NlaIV GGNNCC 2 cut(s) 77, 667
NmuCI GTSAC 1 cut(s) 592
NspI RCATGY 1 cut(s) 26
NspV TTCGAA 1 cut(s) 227
PasI CCCWGGG 1 cut(s) 241
PctI GAATGC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 224, 314
PkrI GCNGC 2 cut(s) 260, 708
PleI GAGTC 2 cut(s) 286, 464
PpsI GAGTC 2 cut(s) 286, 464
PshBI ATTAAT 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 2 cut(s) 77, 667
PstI CTGCAG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 554
RsaI GTAC 2 cut(s) 732, 739
RsaNI GTAC 2 cut(s) 731, 738
SaqAI TTAA 2 cut(s) 41, 528
SatI GCNGC 2 cut(s) 259, 707
Sau3AI GATC 1 cut(s) 554
ScaI AGTACT 1 cut(s) 732
SchI GAGTC 2 cut(s) 287, 465
ScrFI CCNGG 1 cut(s) 242
SetI ASST 6 cut(s) 307, 353, 441, 493, 625, 708
SfcI CTRYAG 1 cut(s) 460
SfuI TTCGAA 1 cut(s) 227
Sse9I AATT 5 cut(s) 38, 67, 266, 341, 660
SspMI CTAG 2 cut(s) 498, 563
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 1 cut(s) 550
TaaI ACNGT 2 cut(s) 143, 592
TaiI ACGT 1 cut(s) 625
TaqI TCGA 2 cut(s) 222, 227
TasI AATT 5 cut(s) 38, 67, 266, 341, 660
TatI WGTACW 2 cut(s) 730, 737
TfiI GAWTC 2 cut(s) 224, 314
Tru1I TTAA 2 cut(s) 41, 528
Tru9I TTAA 2 cut(s) 41, 528
TseFI GTSAC 1 cut(s) 592
TseI GCWGC 2 cut(s) 258, 706
Tsp45I GTSAC 1 cut(s) 592
TspDTI ATGAA 2 cut(s) 306, 324
VspI ATTAAT 1 cut(s) 41
XbaI TCTAGA 1 cut(s) 562
XceI RCATGY 1 cut(s) 26
XspI CTAG 2 cut(s) 498, 563
ZrmI AGTACT 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.