Rh5DG155900

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005181
Physical Location & Seq
Reverse (-)
15848577 .. 15853112
4536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG155900.1

Sequence Viewer

Length: 1005 bp
ATGGCTTCTTCTCTTCCTCTTCATCACCCTTCCCACTGCCTCATCTCCAATCCTCAGTTGCAGGGCTTCAACCATGGAGGCTCACCAAGACCCAGATTCTCTTTCACCACTAAAGACACAGACAAAGGCATTCGCATTCAACCCATTAAAGCCAAAGCAGCCACCACCGAGGCCTCTCCGTTTCCTCTGCTTCAGTCTCCCCGAGCTGAAGACTCTCCCTCTGAGTTAGAGCCAGCAGATCCGGATTTCTACAAGATAGGGTTTGTTAGAAGTATGAGAGCTTATGGAATTGAATTTAAAGAAGGCCCAGATGGGTTTGGAGTTTATGCTTCCAAGGATGTTGAACCACTTCGCCGTGCTAGGGTGATAATGGAAATACCACTAGAACTGATGTTAACTATAAGCCAAAAGCTCCCATGGATGTTTTTCCCAGATATAGTACCGGTGGGTCACCCAATATTTGATATCATCAATTCAACTGATCCAGAGACAGGTTGGGATCTAAGGTTAGCTTGCCTTCTTTTGTATGCATTTGATCGGGAGGATAACTTTTGGCAGTTGTATGGTGATTTTTTACCCAGTGCAGATGAGTGCCCCAGTCTGCTTTTAGCTAGCGAGGAGGAGCTTTCAGAACTACAGGATCCCAATCTTGCTTCAACAATGAGAGAACAGCAACGCCGAGCTCTGGAATTTTGGGAGAAAAATTGGCATTCTGGTGTACCCCTTAAGATTAAACGCCTCGCTCGTGATTCTGAGAGATTCATTTGGGCACTGAGTATGGCACAATCACGATGCATTGGCATGCAAATTAGAATTGGTGCACTTTTTCAAGACTCAAATATGCTAATTCCTTATGCAGACATGCTAAACCATTCCTTCCAGCCAAATTGTTTTCTCCATTGGCGGTTTAAGGATCGTATGCTTGAAGTAATGATAAATGCTGGACAACGGATTAAGAAAGGTGATGAGGTAATATTCGTCCTATATTATGTAATTGTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

334

Amino Acids

38.34

Weight (kDa)

5.68

Isoelectric Point (pI)

52.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 105 - 325 4.5e-08 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 1 cut(s) 904
AclWI GGATC 6 cut(s) 233, 476, 507, 635, 648, 921
AcsI RAATTY 2 cut(s) 293, 689
AcuI CTGAAG 2 cut(s) 176, 228
AfaI GTAC 2 cut(s) 441, 720
AfiI CCNNNNNNNGG 3 cut(s) 361, 491, 685
AflII CTTAAG 1 cut(s) 725
AgeI ACCGGT 1 cut(s) 442
AgsI TTSAA 8 cut(s) 70, 140, 293, 344, 477, 657, 830, 926
AluBI AGCT 7 cut(s) 206, 281, 412, 512, 611, 625, 683
AluI AGCT 7 cut(s) 206, 281, 412, 512, 611, 625, 683
Alw21I GWGCWC 2 cut(s) 685, 823
Alw26I GTCTC 2 cut(s) 201, 482
Alw44I GTGCAC 1 cut(s) 819
AlwI GGATC 6 cut(s) 233, 476, 507, 635, 648, 921
Ama87I CYCGRG 1 cut(s) 201
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 2 cut(s) 171, 304
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 2 cut(s) 293, 689
AsiGI ACCGGT 1 cut(s) 442
Asp700I GAANNNNTTC 1 cut(s) 348
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 7 cut(s) 17, 75, 97, 376, 443, 578, 974
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 1 cut(s) 201
BaeGI GKGCMC 3 cut(s) 596, 772, 823
BamHI GGATCC 1 cut(s) 640
BanII GRGCYC 1 cut(s) 685
BauI CACGAG 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 2 cut(s) 685, 823
BbvI GCAGC 1 cut(s) 170
BccI CCATC 1 cut(s) 305
BceAI ACGGC 1 cut(s) 339
BcoDI GTCTC 2 cut(s) 201, 482
BfaI CTAG 3 cut(s) 360, 383, 612
BfmI CTRYAG 1 cut(s) 635
BfrI CTTAAG 1 cut(s) 725
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 201
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 642
BmrI ACTGGG 2 cut(s) 573, 591
BmsI GCATC 1 cut(s) 782
BmtI GCTAGC 1 cut(s) 615
BmuI ACTGGG 2 cut(s) 573, 591
BpiI GAAGAC 1 cut(s) 216
BsaBI GATNNNNATC 1 cut(s) 645
BsaJI CCNNGG 4 cut(s) 73, 168, 333, 416
BsaWI WCCGGW 2 cut(s) 241, 442
Bsc4I CCNNNNNNNGG 3 cut(s) 361, 491, 685
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 2 cut(s) 579, 597
Bse8I GATNNNNATC 1 cut(s) 645
BseAI TCCGGA 1 cut(s) 241
BseDI CCNNGG 4 cut(s) 73, 168, 333, 416
BseGI GGATG 2 cut(s) 343, 426
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 3 cut(s) 361, 491, 685
BseMII CTCAG 4 cut(s) 68, 213, 744, 764
BseNI ACTGG 2 cut(s) 579, 597
BseRI GAGGAG 2 cut(s) 632, 635
BseSI GKGCMC 3 cut(s) 596, 772, 823
BseXI GCAGC 1 cut(s) 170
BsgI GTGCAG 1 cut(s) 603
BshFI GGCC 2 cut(s) 173, 306
BshTI ACCGGT 1 cut(s) 442
BsiHKAI GWGCWC 2 cut(s) 685, 823
BsiHKCI CYCGRG 1 cut(s) 201
BsiSI CCGG 2 cut(s) 242, 443
BslI CCNNNNNNNGG 3 cut(s) 361, 491, 685
BsmAI GTCTC 2 cut(s) 201, 482
BsmI GAATGC 3 cut(s) 129, 135, 709
BsnI GGCC 2 cut(s) 173, 306
BsoBI CYCGRG 1 cut(s) 201
Bsp1286I GDGCHC 4 cut(s) 596, 685, 772, 823
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 6 cut(s) 238, 481, 499, 535, 640, 913
Bsp19I CCATGG 2 cut(s) 73, 416
BspACI CCGC 1 cut(s) 904
BspANI GGCC 2 cut(s) 173, 306
BspCNI CTCAG 4 cut(s) 67, 214, 745, 765
BspEI TCCGGA 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 642
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 6 cut(s) 233, 476, 507, 635, 648, 921
BspTI CTTAAG 1 cut(s) 725
BsrFI RCCGGY 1 cut(s) 442
BsrI ACTGG 2 cut(s) 579, 597
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 4 cut(s) 73, 168, 333, 416
BssMI GATC 6 cut(s) 238, 481, 499, 535, 640, 913
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 3 cut(s) 73, 333, 416
Bst2BI CACGAG 1 cut(s) 744
Bst6I CTCTTC 2 cut(s) 18, 24
BstAFI CTTAAG 1 cut(s) 725
BstC8I GCNNGC 4 cut(s) 234, 514, 613, 803
BstDEI CTNAG 5 cut(s) 54, 222, 503, 753, 773
BstDSI CCRYGG 2 cut(s) 73, 416
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 2 cut(s) 343, 426
BstKTI GATC 6 cut(s) 241, 484, 502, 538, 643, 916
BstMAI GTCTC 2 cut(s) 201, 482
BstMBI GATC 6 cut(s) 238, 481, 499, 535, 640, 913
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNSI RCATGY 2 cut(s) 805, 865
BstPI GGTNACC 1 cut(s) 449
BstSFI CTRYAG 1 cut(s) 635
BstSLI GKGCMC 3 cut(s) 596, 772, 823
BstV1I GCAGC 1 cut(s) 170
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 3 cut(s) 238, 499, 640
BstYI RGATCY 3 cut(s) 238, 499, 640
BsuRI GGCC 2 cut(s) 173, 306
BtgI CCRYGG 2 cut(s) 73, 416
BtsCI GGATG 2 cut(s) 343, 426
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 3 cut(s) 34, 586, 770
Cac8I GCNNGC 4 cut(s) 234, 514, 613, 803
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 305
Csp6I GTAC 2 cut(s) 440, 719
CspAI ACCGGT 1 cut(s) 442
CviAII CATG 4 cut(s) 74, 417, 802, 862
CviQI GTAC 2 cut(s) 440, 719
DdeI CTNAG 5 cut(s) 54, 222, 503, 753, 773
DpnI GATC 6 cut(s) 240, 483, 501, 537, 642, 915
DpnII GATC 6 cut(s) 238, 481, 499, 535, 640, 913
DraI TTTAAA 1 cut(s) 298
Eam1104I CTCTTC 2 cut(s) 18, 24
EarI CTCTTC 2 cut(s) 18, 24
Ecl136II GAGCTC 1 cut(s) 683
Eco130I CCWWGG 3 cut(s) 73, 333, 416
Eco147I AGGCCT 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 685
Eco32I GATATC 1 cut(s) 466
Eco53kI GAGCTC 1 cut(s) 683
Eco57I CTGAAG 2 cut(s) 176, 228
Eco88I CYCGRG 1 cut(s) 201
Eco91I GGTNACC 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 683
EcoO65I GGTNACC 1 cut(s) 449
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 3 cut(s) 73, 333, 416
EcoT22I ATGCAT 2 cut(s) 532, 797
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 3 cut(s) 73, 333, 416
FaeI CATG 4 cut(s) 77, 420, 805, 865
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FatI CATG 4 cut(s) 73, 416, 801, 861
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 2 cut(s) 350, 433
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 3 cut(s) 360, 383, 612
GluI GCNGC 1 cut(s) 159
HaeIII GGCC 2 cut(s) 173, 306
HapII CCGG 2 cut(s) 242, 443
Hin1II CATG 4 cut(s) 77, 420, 805, 865
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 5 cut(s) 96, 212, 749, 759, 833
HpaI GTTAAC 1 cut(s) 396
HpaII CCGG 2 cut(s) 242, 443
HphI GGTGA 7 cut(s) 17, 75, 97, 376, 443, 578, 974
Hpy166II GTNNAC 3 cut(s) 396, 719, 821
Hpy188I TCNGA 3 cut(s) 223, 631, 754
Hpy188III TCNNGA 7 cut(s) 242, 485, 539, 686, 746, 789, 830
Hpy8I GTNNAC 3 cut(s) 396, 719, 821
HpyAV CCTTC 4 cut(s) 39, 296, 527, 886
HpyCH4V TGCA 7 cut(s) 61, 530, 584, 795, 805, 821, 857
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 5 cut(s) 54, 222, 503, 753, 773
Hsp92II CATG 4 cut(s) 77, 420, 805, 865
Kpn2I TCCGGA 1 cut(s) 241
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 6 cut(s) 238, 481, 499, 535, 640, 913
LmnI GCTCC 2 cut(s) 417, 622
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 1 cut(s) 782
MaeI CTAG 3 cut(s) 360, 383, 612
MaeIII GTNAC 1 cut(s) 449
MalI GATC 6 cut(s) 240, 483, 501, 537, 642, 915
MboI GATC 6 cut(s) 238, 481, 499, 535, 640, 913
MboII GAAGA 3 cut(s) 5, 11, 221
MflI RGATCY 3 cut(s) 238, 499, 640
MhlI GDGCHC 4 cut(s) 596, 685, 772, 823
MlyI GAGTC 2 cut(s) 206, 827
Mph1103I ATGCAT 2 cut(s) 532, 797
MroI TCCGGA 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
MslI CAYNNNNRTG 2 cut(s) 714, 800
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 2 cut(s) 242, 443
Mva1269I GAATGC 3 cut(s) 129, 135, 709
MwoI GCNNNNNNNGC 1 cut(s) 158
NcoI CCATGG 2 cut(s) 73, 416
NdeII GATC 6 cut(s) 238, 481, 499, 535, 640, 913
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 4 cut(s) 77, 420, 805, 865
NlaIV GGNNCC 1 cut(s) 642
NmeAIII GCCGAG 1 cut(s) 704
NmuCI GTSAC 1 cut(s) 449
NsiI ATGCAT 2 cut(s) 532, 797
NspI RCATGY 2 cut(s) 805, 865
PaeI GCATGC 1 cut(s) 805
PceI AGGCCT 1 cut(s) 173
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PctI GAATGC 3 cut(s) 129, 135, 709
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 96, 749, 759
PinAI ACCGGT 1 cut(s) 442
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 2 cut(s) 206, 827
PpsI GAGTC 2 cut(s) 206, 827
Psp124BI GAGCTC 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 449
PspN4I GGNNCC 1 cut(s) 642
PspPI GGNCC 1 cut(s) 305
PsuI RGATCY 3 cut(s) 238, 499, 640
RsaI GTAC 2 cut(s) 441, 720
RsaNI GTAC 2 cut(s) 440, 719
RseI CAYNNNNRTG 2 cut(s) 714, 800
SacI GAGCTC 1 cut(s) 685
SaqAI TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 6 cut(s) 238, 481, 499, 535, 640, 913
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 2 cut(s) 206, 827
SduI GDGCHC 4 cut(s) 596, 685, 772, 823
SfaNI GCATC 1 cut(s) 782
SfcI CTRYAG 1 cut(s) 635
SmiMI CAYNNNNRTG 2 cut(s) 714, 800
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SphI GCATGC 1 cut(s) 805
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 1 cut(s) 904
SspI AATATT 2 cut(s) 459, 975
SspMI CTAG 3 cut(s) 360, 383, 612
SstI GAGCTC 1 cut(s) 685
StuI AGGCCT 1 cut(s) 173
StyI CCWWGG 3 cut(s) 73, 333, 416
TfiI GAWTC 3 cut(s) 96, 749, 759
Tru1I TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
Tru9I TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
TscAI CASTG 3 cut(s) 41, 586, 777
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 2 cut(s) 11, 751
TspGWI ACGGA 2 cut(s) 168, 964
TspRI CASTG 3 cut(s) 41, 586, 777
Vha464I CTTAAG 1 cut(s) 725
VneI GTGCAC 1 cut(s) 819
XapI RAATTY 2 cut(s) 293, 689
XceI RCATGY 2 cut(s) 805, 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 3 cut(s) 360, 383, 612
Zsp2I ATGCAT 2 cut(s) 532, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.