Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0005181

Physical Location & Seq

Genomic Coordinates Reverse (-)

8605689 .. 8625367

Gene Structure 19679 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0064800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2508 bp

ATGGAGGATGGTGACTGGGTTCTGGTGCGGCCGCCTGCAGAGAAGGACCTCTGGACCCCAAGTTTAGTGAAGGAGGATGAGAGTTCTAAGCCTTTGAAGGTTACTTTTGGTGGGCCTGCGAAGTTCTGGACTGATGCAATTCCAATTGGGAATGGGAGGCTTGGAGCTATGGTCTGGGGTGGTGTGCCTTCAGAAACTCTTCAGCTCAATGAGGACACACTCTGGACTGGAACTCCGGGCAACTACACCAACCCAAATGCTCCACAGGCACTAACAGAGGTTAGGAAACTTGTTGATGATGGTAAATATGTTGAAGCTACTGAAGCTGCGGTCAAGTTGTCCGGAGATCCTTCTGATGTATACCAACTACTCGGCGACATCAACCTAGAATTTGAGGATTCGCATCTCAAGTATGCTGAAGAAACTTACAGCAGAGAGCTAGACCTAGATACTGCAACAGCAAGGATAAAATATTCTGTGGGTGATGTAGAATATACTAGGGAGCATTTTGCTTCGAATCCCGATCAAGTGATTGTGACAAAGATCTCTGCAAGTAAATCAGGGTCCCTATCATTCACAGTTTCTCTAGACAGCAAGATGCATCATGGTTCACATTTAAATGGTAAAAACCAAATTATACTTGAAGGAAGTTGTCCTGGTAGAAGAATTGCACCAAAGTATAATGACAATCCAGAGGGGATTCAGTTTACTGCAGTTCTTGATTTGCAGATTAGTGGTGACAAAGGTGTAATACATGCTTTGGATGACAAGAAATTTAGAGTTGAAGAGGCAGATTGGGCTGTTTTGCTTCTGGTGGCCTCATCTTCATTTAAGGATGCATTTACTAAGCCTTCTGATTCTAAGAGAAATCCAACTTCAGAGTCTCTCACTGCCTTGAATTCAATAAGAAATTTGTCATATTCTGACCTCTATGCTCATCATTTGGATGACTATCAGAATCTTTTTCATCGTGTCTCATTGCAGCTCTCTAAGAGCTCTAAGAGCATATTAGGAAATAAAACTTTGGAGATGAAGAAGCTCAACCCTATTACCAATTTGAATTTCAAGAGAAGTGACAATGCCTTGGGTTCTACTGCTGACAGGGTGAATTCCTTTAAAACTGATGAAGATCCTTCTTTTGTGGAACTCTTGTTCCAATATGGTCGATATCTCCTTATTTCATGCTCACGGCCTGGAACTCAGCCGGCAAACCTGCAGGGTATATGGAACTACAAAATTGAACCTGCATGGGATGGTGCTCCTCATACGAACATTAATCTTCAGATGAACTATTGGCCTTCCCTTCCTTGTAATCTACGAGAATGCCAGGAGCCCTTATTTGATTTCACATCTTTATTATCTATTAACGGAAGTAAAACAGCAAAGGTCAACTATGAAGCTAGTGGTTGGGTTGTGCATCAAGTGACTGACATATGGGCGAAAACATCACCAGATCGAGGTCAAGCTGTATGGGCTTTATGGCCAATGGGAGGGGCGTGGATCTGTACTCATCTATGGGAACATTATACTTATACAATGGACAAGGATTTCCTGAAGAATAAGGCATATCCTTTGTTGGAAGGATGTGCATCATTTCTGCTGGACTGGTTGATGGAAGGACGTGGTGGGTATCTTGAAACCAACCCATCAACTTCTCCAGAGCACATGTTTATTGCTCCTGATGGTAAGCAGGCCAGCGTGAGCTACTCATCAACCATGGACATCTCAATCATAAAAGAAGTCTTTTCTGCAGTACTTTCTGCTGCTGAGGTTCTAGGAAAAACTCAAGATACTTTTGTTGAAAAAGTTCGCAATGCTCAATCTAGGCTTCCACCCACAAAAATTGCTAGAGATGGTTCTATTATGGAATGGGCACAAGATTTTGAGGACCCAGAAGTGCACCATCGACATGTATCACACCTGTTTGGCCTGTTTCCGGGCCACACAGTAACTCTTGAAAAAACTCCAGACCTCTGTAAAGCAGTGGATTATACCCTTTTTAAAAGAGGAGAGGAAGGTCCAGGATGGTCAACTACCTGGAAGACTGCATTGTGGGCACGTCTTCACAACAGTGAACATGCGTATCGTATGGTCAAGCATTTGATTGACTTGGTAGATCCAGAACATGAAAGTAATTTTGAAGGAGGACTATACAGTAACTTGTTCACTGCACATCCTCCTTTCCAGATTGATGCCAACTTTGGTTTTTCAGCAGCAGTTGCCGAAATGCTTGTCCAAAGCACAGTCAAGGACCTGTATTTACTTCCTGCTCTTCCTCGGGATAAATGGGCAAATGGTTGTGTCAAGGGCTTGAAGGCACGCGGTGGGGTGACGGTCAACATCTGCTGGAGTGAAGGAGATCTTCACGAAGTTGGCATTTGGTCCAAGGACAACAACACTGTTAAAAAACTACATTATAGAGGAAATACAGTTACGGCAAACATATCGTCGGGCAGGATTTATACATTCAACAGACAGTTAAAATGTGTGAGGACAGTAGGTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000288 GO:0000291 GO:0000785 GO:0000956 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0034655 GO:0035327 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0055087 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPR_1 TPR_16 TPR_19 TPR_2 TPR_7 TPR_8

Protein Analysis

835

Amino Acids

93.3

Weight (kDa)

5.83

Isoelectric Point (pI)

28.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hyd_65N_2	PF14498	36 - 279	1.7e-63	Glycosyl hydrolase family 65, N-terminal domain
Glyco_hydro_95_cat	PF22124	306 - 744	2.7e-168	Glycosyl hydrolase family 95 catalytic domain
Glyco_hydro_95_C	PF21307	746 - 808	1.6e-14	Glycoside hydrolase family 95, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005181)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g13130
malus_domestica	MD05G1244700.v1.1
pyrus_communis	pycom05g22120
rosa_chinensis	RchiOBHm_Chr1g0331441 RchiOBHm_Chr1g0378801 RchiOBHm_Chr2g0167351 RchiOBHm_Chr3g0456081 RchiOBHm_Chr3g0482001 RchiOBHm_Chr5g0063511
rosa_laevigata	RLG00000008600
rosa_multiflora	Rmu_sc0001208.1_g000033
rosa_roxburghii	Rroxscaffold_4G00300020
rosa_rugosa	Rorug02G0631000 Rorug05G0525600 Rorug05G0556200 Rorug06G0064800 Rorug06G0243200 Rorug07G0201200 Rorug07G0222900
rosa_samantha	Rh1BG283100 Rh1DG314900 Rh3AG245400 Rh3DG323100 Rh5BG155400 Rh5DG155900 Rh6BG246300 Rh7DG405100
rosa_wichuraiana	Rw2G025960 Rw4G010270 Rw5G028080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1221, 1252
AccI	GTMKAC	1 cut(s)	360
AccII	CGCG	1 cut(s)	2322
AccIII	TCCGGA	1 cut(s)	341
AciI	CCGC	4 cut(s)	28, 32, 329, 2322
AclWI	GGATC	4 cut(s)	341, 1124, 1508, 2111
AcoI	YGGCCR	2 cut(s)	29, 1481
AcsI	RAATTY	6 cut(s)	389, 773, 898, 910, 1060, 1108
AcuI	CTGAAG	7 cut(s)	174, 185, 342, 438, 861, 1265, 1574
AdeI	CACNNNGTG	1 cut(s)	2324
AfaI	GTAC	2 cut(s)	1507, 1755
AfiI	CCNNNNNNNGG	3 cut(s)	1457, 1490, 1936
AflIII	ACRYGT	2 cut(s)	1665, 1909
AhdI	GACNNNNNGTC	1 cut(s)	2499
AjiI	CACGTC	2 cut(s)	1622, 2060
AjnI	CCWGG	5 cut(s)	655, 1192, 1326, 2020, 2036
AjuI	GAANNNNNNNTTGG	2 cut(s)	1046, 1078
AleI	CACNNNNGTG	1 cut(s)	2070
AloI	GAACNNNNNNTCC	4 cut(s)	1136, 1137, 1168, 1169
Alw21I	GWGCWC	4 cut(s)	998, 1261, 1665, 1902
Alw26I	GTCTC	2 cut(s)	888, 979
Alw44I	GTGCAC	1 cut(s)	1898
AlwI	GGATC	4 cut(s)	341, 1124, 1508, 2111
Ama87I	CYCGRG	1 cut(s)	2277
Aor13HI	TCCGGA	1 cut(s)	341
AoxI	GGCC	9 cut(s)	29, 113, 816, 1190, 1295, 1481, 1692, 1927, 1939
ApaLI	GTGCAC	1 cut(s)	1898
ApeKI	GCWGC	4 cut(s)	326, 982, 1763, 2213
ApoI	RAATTY	6 cut(s)	389, 773, 898, 910, 1060, 1108
ArsI	GACNNNNNNTTYG	4 cut(s)	2217, 2249, 2432, 2464
AseI	ATTAAT	1 cut(s)	1275
Asp700I	GAANNNNTTC	2 cut(s)	198, 1806
AspS9I	GGNCC	9 cut(s)	46, 54, 113, 564, 1888, 1939, 2018, 2251, 2382
AsuC2I	CCSGG	2 cut(s)	237, 1938
AsuHPI	GGTGA	6 cut(s)	23, 494, 749, 1117, 1440, 2341
AsuII	TTCGAA	1 cut(s)	515
AvaI	CYCGRG	1 cut(s)	2277
AvaII	GGWCC	7 cut(s)	46, 54, 564, 1888, 2018, 2251, 2382
BaeGI	GKGCMC	3 cut(s)	1876, 1902, 2059
BaeI	ACNNNNGTAYC	4 cut(s)	1782, 1815, 2066, 2099
BalI	TGGCCA	1 cut(s)	1483
BanII	GRGCYC	2 cut(s)	998, 1335
BbsI	GAAGAC	2 cut(s)	2048, 2054
Bbv12I	GWGCWC	4 cut(s)	998, 1261, 1665, 1902
BbvCI	CCTCAGC	1 cut(s)	1767
BbvI	GCAGC	4 cut(s)	313, 994, 1750, 2225
BccI	CCATC	9 cut(s)	2, 293, 1247, 1606, 1654, 1676, 1847, 1911, 2019
BceAI	ACGGC	2 cut(s)	1205, 2451
BcgI	CGANNNNNNTGC	2 cut(s)	2427, 2461
BciT130I	CCWGG	5 cut(s)	657, 1194, 1328, 2022, 2038
BcnI	CCSGG	2 cut(s)	237, 1938
BcoDI	GTCTC	2 cut(s)	888, 979
BfaI	CTAG	9 cut(s)	386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
BfmI	CTRYAG	4 cut(s)	36, 711, 1214, 1749
BfuAI	ACCTGC	2 cut(s)	1221, 1252
BglII	AGATCT	2 cut(s)	543, 2359
BisI	GCNGC	6 cut(s)	29, 32, 327, 983, 1764, 2214
BlsI	GCNGC	6 cut(s)	30, 33, 328, 984, 1765, 2215
BmcAI	AGTACT	1 cut(s)	1755
Bme1390I	CCNGG	7 cut(s)	237, 657, 1194, 1328, 1938, 2022, 2038
Bme18I	GGWCC	7 cut(s)	46, 54, 564, 1888, 2018, 2251, 2382
BmeRI	GACNNNNNGTC	1 cut(s)	2499
BmeT110I	CYCGRG	1 cut(s)	2277
BmgBI	CACGTC	2 cut(s)	1622, 2060
BmgT120I	GGNCC	9 cut(s)	46, 54, 113, 564, 1888, 1939, 2018, 2251, 2382
BmiI	GGNNCC	5 cut(s)	56, 565, 566, 1332, 1890
BmrFI	CCNGG	7 cut(s)	237, 657, 1194, 1328, 1938, 2022, 2038
BmrI	ACTGGG	1 cut(s)	25
BmsI	GCATC	8 cut(s)	124, 412, 588, 610, 826, 1426, 1598, 2182
BmuI	ACTGGG	1 cut(s)	25
BpiI	GAAGAC	2 cut(s)	2048, 2054
BpmI	CTGGAG	3 cut(s)	1641, 1950, 2368
Bpu10I	CCTNAGC	1 cut(s)	1767
Bpu14I	TTCGAA	1 cut(s)	515
BpuEI	CTTGAG	2 cut(s)	392, 1770
BpuMI	CCSGG	2 cut(s)	237, 1938
BsaBI	GATNNNNATC	4 cut(s)	402, 951, 1128, 1588
BsaJI	CCNNGG	4 cut(s)	1083, 1716, 2276, 2385
BsaWI	WCCGGW	1 cut(s)	341
BsaXI	ACNNNNNCTCC	4 cut(s)	156, 186, 217, 247
Bsc4I	CCNNNNNNNGG	3 cut(s)	1457, 1490, 1936
Bse118I	RCCGGY	1 cut(s)	1204
Bse1I	ACTGG	3 cut(s)	20, 232, 1610
Bse3DI	GCAATG	2 cut(s)	977, 1819
Bse8I	GATNNNNATC	4 cut(s)	402, 951, 1128, 1588
BseAI	TCCGGA	1 cut(s)	341
BseBI	CCWGG	5 cut(s)	657, 1194, 1328, 2022, 2038
BseDI	CCNNGG	4 cut(s)	1083, 1716, 2276, 2385
BseGI	GGATG	9 cut(s)	13, 82, 769, 841, 952, 1258, 1589, 2030, 2173
BseJI	GATNNNNATC	4 cut(s)	402, 951, 1128, 1588
BseLI	CCNNNNNNNGG	3 cut(s)	1457, 1490, 1936
BseMI	GCAATG	2 cut(s)	977, 1819
BseMII	CTCAG	2 cut(s)	1214, 1758
BseNI	ACTGG	3 cut(s)	20, 232, 1610
BseRI	GAGGAG	2 cut(s)	1251, 2022
BseSI	GKGCMC	3 cut(s)	1876, 1902, 2059
BseX3I	CGGCCG	1 cut(s)	29
BseXI	GCAGC	4 cut(s)	313, 994, 1750, 2225
BsgI	GTGCAG	1 cut(s)	2154
Bsh1236I	CGCG	1 cut(s)	2322
Bsh1285I	CGRYCG	1 cut(s)	32
BshFI	GGCC	9 cut(s)	31, 115, 818, 1192, 1297, 1483, 1694, 1929, 1941
BsiEI	CGRYCG	1 cut(s)	32
BsiHKAI	GWGCWC	4 cut(s)	998, 1261, 1665, 1902
BsiHKCI	CYCGRG	1 cut(s)	2277
BsiSI	CCGG	4 cut(s)	236, 342, 1205, 1937
BslFI	GGGAC	1 cut(s)	550
BslI	CCNNNNNNNGG	3 cut(s)	1457, 1490, 1936
BsmAI	GTCTC	2 cut(s)	888, 979
BsmFI	GGGAC	1 cut(s)	550
BsmI	GAATGC	1 cut(s)	1328
BsnI	GGCC	9 cut(s)	31, 115, 818, 1192, 1297, 1483, 1694, 1929, 1941
BsoBI	CYCGRG	1 cut(s)	2277
Bsp119I	TTCGAA	1 cut(s)	515
Bsp1286I	GDGCHC	7 cut(s)	998, 1261, 1335, 1665, 1876, 1902, 2059
Bsp13I	TCCGGA	1 cut(s)	341
Bsp143I	GATC	8 cut(s)	346, 523, 543, 1129, 1453, 1500, 2116, 2359
Bsp19I	CCATGG	1 cut(s)	1716
BspACI	CCGC	4 cut(s)	28, 32, 329, 2322
BspANI	GGCC	9 cut(s)	31, 115, 818, 1192, 1297, 1483, 1694, 1929, 1941
BspCNI	CTCAG	2 cut(s)	1213, 1759
BspEI	TCCGGA	1 cut(s)	341
BspFNI	CGCG	1 cut(s)	2322
BspLI	GGNNCC	5 cut(s)	56, 565, 566, 1332, 1890
BspMAI	CTGCAG	4 cut(s)	40, 715, 1218, 1753
BspMI	ACCTGC	2 cut(s)	1221, 1252
BspPI	GGATC	4 cut(s)	341, 1124, 1508, 2111
BspQI	GCTCTTC	1 cut(s)	2277
BspT104I	TTCGAA	1 cut(s)	515
BsrDI	GCAATG	2 cut(s)	977, 1819
BsrFI	RCCGGY	1 cut(s)	1204
BsrI	ACTGG	3 cut(s)	20, 232, 1610
BssAI	RCCGGY	1 cut(s)	1204
BssECI	CCNNGG	4 cut(s)	1083, 1716, 2276, 2385
BssMI	GATC	8 cut(s)	346, 523, 543, 1129, 1453, 1500, 2116, 2359
BssNAI	GTATAC	1 cut(s)	361
BssT1I	CCWWGG	3 cut(s)	1083, 1716, 2385
Bst1107I	GTATAC	1 cut(s)	361
Bst2UI	CCWGG	5 cut(s)	657, 1194, 1328, 2022, 2038
Bst6I	CTCTTC	3 cut(s)	204, 780, 2277
BstAPI	GCANNNNNTGC	1 cut(s)	2219
BstBI	TTCGAA	1 cut(s)	515
BstC8I	GCNNGC	6 cut(s)	36, 117, 1206, 1692, 1696, 2320
BstDEI	CTNAG	7 cut(s)	87, 846, 861, 990, 999, 1200, 1767
BstDSI	CCRYGG	1 cut(s)	1716
BstF5I	GGATG	9 cut(s)	13, 82, 769, 841, 952, 1258, 1589, 2030, 2173
BstFNI	CGCG	1 cut(s)	2322
BstKTI	GATC	8 cut(s)	349, 526, 546, 1132, 1456, 1503, 2119, 2362
BstMAI	GTCTC	2 cut(s)	888, 979
BstMBI	GATC	8 cut(s)	346, 523, 543, 1129, 1453, 1500, 2116, 2359
BstMCI	CGRYCG	1 cut(s)	32
BstMWI	GCNNNNNNNGC	7 cut(s)	266, 323, 797, 1002, 1472, 2054, 2219
BstNI	CCWGG	5 cut(s)	657, 1194, 1328, 2022, 2038
BstNSI	RCATGY	4 cut(s)	758, 1669, 1913, 2081
BstSCI	CCNGG	7 cut(s)	235, 655, 1192, 1326, 1936, 2020, 2036
BstSFI	CTRYAG	4 cut(s)	36, 711, 1214, 1749
BstSLI	GKGCMC	3 cut(s)	1876, 1902, 2059
BstUI	CGCG	1 cut(s)	2322
BstV1I	GCAGC	4 cut(s)	313, 994, 1750, 2225
BstV2I	GAAGAC	2 cut(s)	2048, 2054
BstX2I	RGATCY	6 cut(s)	346, 543, 1129, 1500, 2116, 2359
BstYI	RGATCY	6 cut(s)	346, 543, 1129, 1500, 2116, 2359
BstZ17I	GTATAC	1 cut(s)	361
BstZI	CGGCCG	1 cut(s)	29
BsuRI	GGCC	9 cut(s)	31, 115, 818, 1192, 1297, 1483, 1694, 1929, 1941
BtgI	CCRYGG	1 cut(s)	1716
BtrI	CACGTC	2 cut(s)	1622, 2060
BtsCI	GGATG	9 cut(s)	13, 82, 769, 841, 952, 1258, 1589, 2030, 2173
BtsI	GCAGTG	3 cut(s)	888, 1989, 2166
BtsIMutI	CAGTG	5 cut(s)	888, 1989, 2077, 2166, 2397
BveI	ACCTGC	2 cut(s)	1221, 1252
Cac8I	GCNNGC	6 cut(s)	36, 117, 1206, 1692, 1696, 2320
CciNI	GCGGCCGC	1 cut(s)	29
Cfr10I	RCCGGY	1 cut(s)	1204
Cfr13I	GGNCC	9 cut(s)	46, 54, 113, 564, 1888, 1939, 2018, 2251, 2382
Csp6I	GTAC	2 cut(s)	1506, 1754
CspCI	CAANNNNNGTGG	2 cut(s)	1825, 1860
CviAII	CATG	9 cut(s)	605, 755, 1182, 1248, 1666, 1717, 1910, 2078, 2126
CviQI	GTAC	2 cut(s)	1506, 1754
DdeI	CTNAG	7 cut(s)	87, 846, 861, 990, 999, 1200, 1767
DpnI	GATC	8 cut(s)	348, 525, 545, 1131, 1455, 1502, 2118, 2361
DpnII	GATC	8 cut(s)	346, 523, 543, 1129, 1453, 1500, 2116, 2359
DraI	TTTAAA	3 cut(s)	618, 1117, 2002
DraIII	CACNNNGTG	1 cut(s)	2324
DriI	GACNNNNNGTC	1 cut(s)	2499
EaeI	YGGCCR	2 cut(s)	29, 1481
EagI	CGGCCG	1 cut(s)	29
Eam1104I	CTCTTC	3 cut(s)	204, 780, 2277
Eam1105I	GACNNNNNGTC	1 cut(s)	2499
EarI	CTCTTC	3 cut(s)	204, 780, 2277
Ecl136II	GAGCTC	1 cut(s)	996
EclXI	CGGCCG	1 cut(s)	29
Eco130I	CCWWGG	3 cut(s)	1083, 1716, 2385
Eco24I	GRGCYC	2 cut(s)	998, 1335
Eco32I	GATATC	1 cut(s)	1169
Eco47I	GGWCC	7 cut(s)	46, 54, 564, 1888, 2018, 2251, 2382
Eco52I	CGGCCG	1 cut(s)	29
Eco53kI	GAGCTC	1 cut(s)	996
Eco57I	CTGAAG	7 cut(s)	174, 185, 342, 438, 861, 1265, 1574
Eco88I	CYCGRG	1 cut(s)	2277
EcoICRI	GAGCTC	1 cut(s)	996
EcoO109I	RGGNCCY	4 cut(s)	46, 564, 1888, 2251
EcoRI	GAATTC	2 cut(s)	898, 1108
EcoRII	CCWGG	5 cut(s)	655, 1192, 1326, 2020, 2036
EcoRV	GATATC	1 cut(s)	1169
EcoT14I	CCWWGG	3 cut(s)	1083, 1716, 2385
EcoT22I	ATGCAT	2 cut(s)	603, 841
EcoT38I	GRGCYC	2 cut(s)	998, 1335
ErhI	CCWWGG	3 cut(s)	1083, 1716, 2385
FaeI	CATG	9 cut(s)	608, 758, 1185, 1251, 1669, 1720, 1913, 2081, 2129
FalI	AAGNNNNNCTT	2 cut(s)	2346, 2378
FaqI	GGGAC	1 cut(s)	550
FatI	CATG	9 cut(s)	604, 754, 1181, 1247, 1665, 1716, 1909, 2077, 2125
FauNDI	CATATG	1 cut(s)	1433
FblI	GTMKAC	1 cut(s)	360
Fnu4HI	GCNGC	6 cut(s)	29, 32, 327, 983, 1764, 2214
FokI	GGATG	9 cut(s)	20, 89, 776, 848, 959, 1265, 1596, 2037, 2160
FriOI	GRGCYC	2 cut(s)	998, 1335
Fsp4HI	GCNGC	6 cut(s)	29, 32, 327, 983, 1764, 2214
FspBI	CTAG	9 cut(s)	386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
GluI	GCNGC	6 cut(s)	29, 32, 327, 983, 1764, 2214
GsuI	CTGGAG	3 cut(s)	1641, 1950, 2368
HaeIII	GGCC	9 cut(s)	31, 115, 818, 1192, 1297, 1483, 1694, 1929, 1941
HapII	CCGG	4 cut(s)	236, 342, 1205, 1937
Hin1II	CATG	9 cut(s)	608, 758, 1185, 1251, 1669, 1720, 1913, 2081, 2129
HincII	GTYRAC	3 cut(s)	1390, 2031, 2338
HindII	GTYRAC	3 cut(s)	1390, 2031, 2338
HinfI	GANTC	6 cut(s)	398, 517, 700, 857, 881, 958
HpaII	CCGG	4 cut(s)	236, 342, 1205, 1937
HphI	GGTGA	6 cut(s)	23, 494, 749, 1117, 1440, 2341
Hpy166II	GTNNAC	9 cut(s)	361, 611, 708, 1390, 1900, 2031, 2075, 2166, 2338
Hpy188I	TCNGA	7 cut(s)	193, 355, 856, 880, 925, 957, 1284
Hpy8I	GTNNAC	9 cut(s)	361, 611, 708, 1390, 1900, 2031, 2075, 2166, 2338
Hpy99I	CGWCG	1 cut(s)	2452
HpyCH4IV	ACGT	2 cut(s)	1621, 2059
HpyF10VI	GCNNNNNNNGC	7 cut(s)	266, 323, 797, 1002, 1472, 2054, 2219
HpyF3I	CTNAG	7 cut(s)	87, 846, 861, 990, 999, 1200, 1767
HpySE526I	ACGT	2 cut(s)	1621, 2059
Hsp92II	CATG	9 cut(s)	608, 758, 1185, 1251, 1669, 1720, 1913, 2081, 2129
KflI	GGGWCCC	1 cut(s)	564
Kpn2I	TCCGGA	1 cut(s)	341
KroI	GCCGGC	1 cut(s)	1204
KroNI	GCCGGC	1 cut(s)	1206
Kzo9I	GATC	8 cut(s)	346, 523, 543, 1129, 1453, 1500, 2116, 2359
LguI	GCTCTTC	1 cut(s)	2277
LmnI	GCTCC	6 cut(s)	164, 265, 502, 1264, 1330, 1681
Lsp1109I	GCAGC	4 cut(s)	313, 994, 1750, 2225
LweI	GCATC	8 cut(s)	124, 412, 588, 610, 826, 1426, 1598, 2182
MaeI	CTAG	9 cut(s)	386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
MaeII	ACGT	2 cut(s)	1621, 2059
MalI	GATC	8 cut(s)	348, 525, 545, 1131, 1455, 1502, 2118, 2361
MboI	GATC	8 cut(s)	346, 523, 543, 1129, 1453, 1500, 2116, 2359
MfeI	CAATTG	1 cut(s)	144
MflI	RGATCY	6 cut(s)	346, 543, 1129, 1500, 2116, 2359
MhlI	GDGCHC	7 cut(s)	998, 1261, 1335, 1665, 1876, 1902, 2059
MlsI	TGGCCA	1 cut(s)	1483
MluNI	TGGCCA	1 cut(s)	1483
MlyI	GAGTC	1 cut(s)	890
MmeI	TCCRAC	2 cut(s)	896, 1557
Mox20I	TGGCCA	1 cut(s)	1483
Mph1103I	ATGCAT	2 cut(s)	603, 841
MroI	TCCGGA	1 cut(s)	341
MroNI	GCCGGC	1 cut(s)	1204
MroXI	GAANNNNTTC	2 cut(s)	198, 1806
MscI	TGGCCA	1 cut(s)	1483
MseI	TTAA	8 cut(s)	617, 831, 1116, 1275, 1365, 2001, 2403, 2480
MslI	CAYNNNNRTG	4 cut(s)	618, 945, 1908, 2070
Msp20I	TGGCCA	1 cut(s)	1483
MspI	CCGG	4 cut(s)	236, 342, 1205, 1937
MspR9I	CCNGG	7 cut(s)	237, 657, 1194, 1328, 1938, 2022, 2038
MunI	CAATTG	1 cut(s)	144
Mva1269I	GAATGC	1 cut(s)	1328
MvaI	CCWGG	5 cut(s)	657, 1194, 1328, 2022, 2038
MvnI	CGCG	1 cut(s)	2322
MwoI	GCNNNNNNNGC	7 cut(s)	266, 323, 797, 1002, 1472, 2054, 2219
NaeI	GCCGGC	1 cut(s)	1206
NciI	CCSGG	2 cut(s)	237, 1938
NcoI	CCATGG	1 cut(s)	1716
NdeI	CATATG	1 cut(s)	1433
NdeII	GATC	8 cut(s)	346, 523, 543, 1129, 1453, 1500, 2116, 2359
NgoMIV	GCCGGC	1 cut(s)	1204
NlaIII	CATG	9 cut(s)	608, 758, 1185, 1251, 1669, 1720, 1913, 2081, 2129
NlaIV	GGNNCC	5 cut(s)	56, 565, 566, 1332, 1890
NmeAIII	GCCGAG	1 cut(s)	351
NmuCI	GTSAC	6 cut(s)	11, 535, 737, 1073, 1423, 2329
NotI	GCGGCCGC	1 cut(s)	29
NsiI	ATGCAT	2 cut(s)	603, 841
NspI	RCATGY	4 cut(s)	758, 1669, 1913, 2081
NspV	TTCGAA	1 cut(s)	515
OliI	CACNNNNGTG	1 cut(s)	2070
PciI	ACATGT	2 cut(s)	1665, 1909
PciSI	GCTCTTC	1 cut(s)	2277
PctI	GAATGC	1 cut(s)	1328
PdiI	GCCGGC	1 cut(s)	1206
PdmI	GAANNNNTTC	2 cut(s)	198, 1806
PfeI	GAWTC	5 cut(s)	398, 517, 700, 857, 958
PfoI	TCCNGGA	1 cut(s)	2020
PkrI	GCNGC	6 cut(s)	30, 33, 328, 984, 1765, 2215
PleI	GAGTC	1 cut(s)	889
PpsI	GAGTC	1 cut(s)	889
PpuMI	RGGWCCY	4 cut(s)	46, 564, 1888, 2251
PscI	ACATGT	2 cut(s)	1665, 1909
PshBI	ATTAAT	1 cut(s)	1275
Psp124BI	GAGCTC	1 cut(s)	998
Psp5II	RGGWCCY	4 cut(s)	46, 564, 1888, 2251
Psp6I	CCWGG	5 cut(s)	655, 1192, 1326, 2020, 2036
PspGI	CCWGG	5 cut(s)	655, 1192, 1326, 2020, 2036
PspN4I	GGNNCC	5 cut(s)	56, 565, 566, 1332, 1890
PspPI	GGNCC	9 cut(s)	46, 54, 113, 564, 1888, 1939, 2018, 2251, 2382
PspPPI	RGGWCCY	4 cut(s)	46, 564, 1888, 2251
PstI	CTGCAG	4 cut(s)	40, 715, 1218, 1753
PsuI	RGATCY	6 cut(s)	346, 543, 1129, 1500, 2116, 2359
RsaI	GTAC	2 cut(s)	1507, 1755
RsaNI	GTAC	2 cut(s)	1506, 1754
RseI	CAYNNNNRTG	4 cut(s)	618, 945, 1908, 2070
SacI	GAGCTC	1 cut(s)	998
SapI	GCTCTTC	1 cut(s)	2277
SaqAI	TTAA	8 cut(s)	617, 831, 1116, 1275, 1365, 2001, 2403, 2480
SatI	GCNGC	6 cut(s)	29, 32, 327, 983, 1764, 2214
Sau3AI	GATC	8 cut(s)	346, 523, 543, 1129, 1453, 1500, 2116, 2359
Sau96I	GGNCC	9 cut(s)	46, 54, 113, 564, 1888, 1939, 2018, 2251, 2382
SbfI	CCTGCAGG	1 cut(s)	1218
ScaI	AGTACT	1 cut(s)	1755
SchI	GAGTC	1 cut(s)	890
ScrFI	CCNGG	7 cut(s)	237, 657, 1194, 1328, 1938, 2022, 2038
SdaI	CCTGCAGG	1 cut(s)	1218
SduI	GDGCHC	7 cut(s)	998, 1261, 1335, 1665, 1876, 1902, 2059
SfaNI	GCATC	8 cut(s)	124, 412, 588, 610, 826, 1426, 1598, 2182
SfcI	CTRYAG	4 cut(s)	36, 711, 1214, 1749
SfuI	TTCGAA	1 cut(s)	515
SinI	GGWCC	7 cut(s)	46, 54, 564, 1888, 2018, 2251, 2382
SmiI	ATTTAAAT	1 cut(s)	618
SmiMI	CAYNNNNRTG	4 cut(s)	618, 945, 1908, 2070
SmlI	CTYRAG	2 cut(s)	407, 1785
SmoI	CTYRAG	2 cut(s)	407, 1785
Sse8387I	CCTGCAGG	1 cut(s)	1218
SsiI	CCGC	4 cut(s)	28, 32, 329, 2322
SspI	AATATT	1 cut(s)	473
SspMI	CTAG	9 cut(s)	386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
SstI	GAGCTC	1 cut(s)	998
StyD4I	CCNGG	7 cut(s)	235, 655, 1192, 1326, 1936, 2020, 2036
StyI	CCWWGG	3 cut(s)	1083, 1716, 2385
SwaI	ATTTAAAT	1 cut(s)	618
TaiI	ACGT	2 cut(s)	1624, 2062
TaqI	TCGA	4 cut(s)	515, 1165, 1456, 1906
TatI	WGTACW	2 cut(s)	1505, 1753
TauI	GCSGC	2 cut(s)	31, 34
TfiI	GAWTC	5 cut(s)	398, 517, 700, 857, 958
Tru1I	TTAA	8 cut(s)	617, 831, 1116, 1275, 1365, 2001, 2403, 2480
Tru9I	TTAA	8 cut(s)	617, 831, 1116, 1275, 1365, 2001, 2403, 2480
TscAI	CASTG	5 cut(s)	895, 1989, 2077, 2173, 2404
TseFI	GTSAC	6 cut(s)	11, 535, 737, 1073, 1423, 2329
TseI	GCWGC	4 cut(s)	326, 982, 1763, 2213
Tsp45I	GTSAC	6 cut(s)	11, 535, 737, 1073, 1423, 2329
TspDTI	ATGAA	8 cut(s)	816, 956, 1046, 1140, 1170, 1301, 1410, 2142
TspGWI	ACGGA	1 cut(s)	1383
TspRI	CASTG	5 cut(s)	895, 1989, 2077, 2173, 2404
VneI	GTGCAC	1 cut(s)	1898
VpaK11BI	GGWCC	7 cut(s)	46, 54, 564, 1888, 2018, 2251, 2382
VspI	ATTAAT	1 cut(s)	1275
XapI	RAATTY	6 cut(s)	389, 773, 898, 910, 1060, 1108
XbaI	TCTAGA	1 cut(s)	586
XceI	RCATGY	4 cut(s)	758, 1669, 1913, 2081
XmiI	GTMKAC	1 cut(s)	360
XmnI	GAANNNNTTC	2 cut(s)	198, 1806
XspI	CTAG	9 cut(s)	386, 440, 446, 498, 587, 1401, 1775, 1824, 1848
ZrmI	AGTACT	1 cut(s)	1755
Zsp2I	ATGCAT	2 cut(s)	603, 841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.