Rh3CG032700

AUX/IAA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0009806
Physical Location & Seq
Reverse (-)
2263467 .. 2266868
3402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG032700.1

Sequence Viewer

Length: 624 bp
ATGACTAGTACTGCTGCTATGGGTGGTGGTGTTACGGAGGAGGATCGGAAGTACAGCTTGATAAACTTCGAAGAGACGGAGCTGCGTCTAGGGTTGCCGGGTCCGCTGAAAGACGGCGACCAAGGCGTGAAGAGCGGTAATGGGAAGAGAGGGTTTTTGGAAACGGTTGATTTGAAGCTCAACTTCTCGTCGGTAAATGATGATGAGCATCGGTCAGCTGGAGATGGTCAGCTGGAGATCAAAAAGGAGAAGGAGGATGCTTCTGCTGCTCCTGCTCCACGTGCAAAGGCTCAAGTTGTGGGTTGGCCACCCGTTCGATCCTTCCGAAAGAATATCGTGACCGTCCAAAAGAAAAGCACAGATCAGGATCAGGCTGAAAAGACCGGCAGCACTAGTACTAGTGCAGCGTTTGTTAAAGTGAGCATGGATGGAGCCCCATATTTGCGCAAAGTCGACTTGAAGTTGTACCAAAGCTACCAAGAGCTTTCTACTGCTCTAGGCAAAATGTTTAGCTCCTTCACTATTGGTAATTCTAAGATTTTACCTATACGTAACCTTTCACCCAACGTCATCCTTTCTTTGTTTACAAGTGGTAATTTTATTTTTTTATGTTTACATGAATGA

Protein Analysis

207

Amino Acids

22.55

Weight (kDa)

8.56

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 25 - 178 3.8e-45 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 446
AccBSI CCGCTC 1 cut(s) 135
AccI GTMKAC 1 cut(s) 453
AciI CCGC 2 cut(s) 104, 135
AclWI GGATC 3 cut(s) 51, 312, 375
AcoI YGGCCR 1 cut(s) 305
AcvI CACGTG 1 cut(s) 281
AfaI GTAC 4 cut(s) 10, 53, 397, 467
AgsI TTSAA 2 cut(s) 175, 460
AhlI ACTAGT 3 cut(s) 5, 392, 398
AluBI AGCT 8 cut(s) 57, 82, 178, 218, 232, 474, 484, 513
AluI AGCT 8 cut(s) 57, 82, 178, 218, 232, 474, 484, 513
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 3 cut(s) 51, 312, 375
AoxI GGCC 1 cut(s) 305
ApeKI GCWGC 5 cut(s) 14, 82, 266, 387, 404
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 552
AsuII TTCGAA 1 cut(s) 69
AvaII GGWCC 1 cut(s) 101
BalI TGGCCA 1 cut(s) 307
BanII GRGCYC 1 cut(s) 436
BbrPI CACGTG 1 cut(s) 281
BbvI GCAGC 4 cut(s) 69, 253, 399, 416
BccI CCATC 2 cut(s) 218, 422
BceAI ACGGC 1 cut(s) 130
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 68
BcuI ACTAGT 3 cut(s) 5, 392, 398
BfaI CTAG 5 cut(s) 6, 89, 393, 399, 497
BisI GCNGC 5 cut(s) 15, 83, 267, 388, 405
BlsI GCNGC 5 cut(s) 16, 84, 268, 389, 406
BmcAI AGTACT 2 cut(s) 10, 397
Bme1390I CCNGG 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 2 cut(s) 102, 433
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 2 cut(s) 217, 247
BpmI CTGGAG 2 cut(s) 240, 254
Bpu14I TTCGAA 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 99
BsaAI YACGTR 2 cut(s) 281, 551
BsaBI GATNNNNATC 2 cut(s) 207, 366
BsaJI CCNNGG 1 cut(s) 121
Bse118I RCCGGY 1 cut(s) 383
Bse8I GATNNNNATC 2 cut(s) 207, 366
BseDI CCNNGG 1 cut(s) 121
BseGI GGATG 3 cut(s) 262, 433, 570
BseJI GATNNNNATC 2 cut(s) 207, 366
BseRI GAGGAG 1 cut(s) 53
BseXI GCAGC 4 cut(s) 69, 253, 399, 416
BsgI GTGCAG 1 cut(s) 423
BshFI GGCC 1 cut(s) 307
BsiSI CCGG 2 cut(s) 98, 384
BsmAI GTCTC 1 cut(s) 68
BsmBI CGTCTC 1 cut(s) 68
BsnI GGCC 1 cut(s) 307
Bsp119I TTCGAA 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 5 cut(s) 43, 237, 317, 361, 367
BspACI CCGC 2 cut(s) 104, 135
BspANI GGCC 1 cut(s) 307
BspLI GGNNCC 2 cut(s) 102, 433
BspPI GGATC 3 cut(s) 51, 312, 375
BspQI GCTCTTC 1 cut(s) 125
BspT104I TTCGAA 1 cut(s) 69
BsrBI CCGCTC 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 383
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 5 cut(s) 43, 237, 317, 361, 367
BssT1I CCWWGG 1 cut(s) 121
Bst4CI ACNGT 2 cut(s) 166, 343
Bst6I CTCTTC 3 cut(s) 66, 125, 140
BstBAI YACGTR 2 cut(s) 281, 551
BstBI TTCGAA 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 534
BstF5I GGATG 3 cut(s) 262, 433, 570
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 5 cut(s) 46, 240, 320, 364, 370
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 5 cut(s) 43, 237, 317, 361, 367
BstMWI GCNNNNNNNGC 6 cut(s) 103, 123, 132, 266, 272, 281
BstSCI CCNGG 1 cut(s) 97
BstSNI TACGTA 1 cut(s) 551
BstV1I GCAGC 4 cut(s) 69, 253, 399, 416
BsuRI GGCC 1 cut(s) 307
BtsCI GGATG 3 cut(s) 262, 433, 570
CfoI GCGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 101
CseI GACGC 1 cut(s) 74
Csp6I GTAC 4 cut(s) 9, 52, 396, 466
CviAII CATG 2 cut(s) 424, 617
CviQI GTAC 4 cut(s) 9, 52, 396, 466
DdeI CTNAG 1 cut(s) 534
DpnI GATC 5 cut(s) 45, 239, 319, 363, 369
DpnII GATC 5 cut(s) 43, 237, 317, 361, 367
EaeI YGGCCR 1 cut(s) 305
Eam1104I CTCTTC 3 cut(s) 66, 125, 140
EarI CTCTTC 3 cut(s) 66, 125, 140
Eco105I TACGTA 1 cut(s) 551
Eco130I CCWWGG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 101
Eco72I CACGTG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 121
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 2 cut(s) 427, 620
FaiI YATR 6 cut(s) 20, 425, 439, 548, 610, 618
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 167, 199
FatI CATG 2 cut(s) 423, 616
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 5 cut(s) 15, 83, 267, 388, 405
FokI GGATG 3 cut(s) 269, 440, 557
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 5 cut(s) 15, 83, 267, 388, 405
FspBI CTAG 5 cut(s) 6, 89, 393, 399, 497
FspI TGCGCA 1 cut(s) 446
GlaI GCGC 1 cut(s) 446
GluI GCNGC 5 cut(s) 15, 83, 267, 388, 405
GsuI CTGGAG 2 cut(s) 240, 254
HaeIII GGCC 1 cut(s) 307
HapII CCGG 2 cut(s) 98, 384
HgaI GACGC 1 cut(s) 74
HhaI GCGC 1 cut(s) 447
Hin1II CATG 2 cut(s) 427, 620
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HpaII CCGG 2 cut(s) 98, 384
HphI GGTGA 1 cut(s) 552
Hpy166II GTNNAC 3 cut(s) 454, 585, 614
Hpy188I TCNGA 2 cut(s) 48, 326
Hpy188III TCNNGA 2 cut(s) 337, 365
Hpy8I GTNNAC 3 cut(s) 454, 585, 614
Hpy99I CGWCG 1 cut(s) 193
HpyAV CCTTC 3 cut(s) 244, 331, 526
HpyCH4III ACNGT 2 cut(s) 166, 343
HpyCH4IV ACGT 3 cut(s) 280, 550, 567
HpyCH4V TGCA 2 cut(s) 284, 404
HpyF10VI GCNNNNNNNGC 6 cut(s) 103, 123, 132, 266, 272, 281
HpyF3I CTNAG 1 cut(s) 534
HpySE526I ACGT 3 cut(s) 280, 550, 567
Hsp92II CATG 2 cut(s) 427, 620
HspAI GCGC 1 cut(s) 445
Kzo9I GATC 5 cut(s) 43, 237, 317, 361, 367
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 5 cut(s) 79, 274, 280, 431, 518
LpnPI CCDG 7 cut(s) 111, 204, 218, 285, 350, 356, 397
Lsp1109I GCAGC 4 cut(s) 69, 253, 399, 416
LweI GCATC 2 cut(s) 217, 247
MaeI CTAG 5 cut(s) 6, 89, 393, 399, 497
MaeII ACGT 3 cut(s) 280, 550, 567
MaeIII GTNAC 3 cut(s) 31, 337, 551
MalI GATC 5 cut(s) 45, 239, 319, 363, 369
MbiI CCGCTC 1 cut(s) 135
MboI GATC 5 cut(s) 43, 237, 317, 361, 367
MboII GAAGA 3 cut(s) 83, 142, 157
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 307
MluCI AATT 2 cut(s) 529, 595
MluNI TGGCCA 1 cut(s) 307
MnlI CCTC 4 cut(s) 31, 34, 143, 247
Mox20I TGGCCA 1 cut(s) 307
MscI TGGCCA 1 cut(s) 307
MseI TTAA 1 cut(s) 414
Msp20I TGGCCA 1 cut(s) 307
MspA1I CMGCKG 3 cut(s) 106, 218, 232
MspI CCGG 2 cut(s) 98, 384
MspR9I CCNGG 1 cut(s) 99
MwoI GCNNNNNNNGC 6 cut(s) 103, 123, 132, 266, 272, 281
NciI CCSGG 1 cut(s) 99
NdeII GATC 5 cut(s) 43, 237, 317, 361, 367
NlaIII CATG 2 cut(s) 427, 620
NlaIV GGNNCC 2 cut(s) 102, 433
NmuCI GTSAC 1 cut(s) 337
NsbI TGCGCA 1 cut(s) 446
NspV TTCGAA 1 cut(s) 69
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 1 cut(s) 322
PkrI GCNGC 5 cut(s) 16, 84, 268, 389, 406
PmaCI CACGTG 1 cut(s) 281
PmlI CACGTG 1 cut(s) 281
Ppu21I YACGTR 2 cut(s) 281, 551
PspCI CACGTG 1 cut(s) 281
PspN4I GGNNCC 2 cut(s) 102, 433
PspPI GGNCC 1 cut(s) 101
PvuII CAGCTG 2 cut(s) 218, 232
RsaI GTAC 4 cut(s) 10, 53, 397, 467
RsaNI GTAC 4 cut(s) 9, 52, 396, 466
SalI GTCGAC 1 cut(s) 452
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 5 cut(s) 15, 83, 267, 388, 405
Sau3AI GATC 5 cut(s) 43, 237, 317, 361, 367
Sau96I GGNCC 1 cut(s) 101
ScaI AGTACT 2 cut(s) 10, 397
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 1 cut(s) 436
SfaNI GCATC 2 cut(s) 217, 247
SfuI TTCGAA 1 cut(s) 69
SinI GGWCC 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 291
SmoI CTYRAG 1 cut(s) 291
SnaBI TACGTA 1 cut(s) 551
SpeI ACTAGT 3 cut(s) 5, 392, 398
Sse9I AATT 2 cut(s) 529, 595
SsiI CCGC 2 cut(s) 104, 135
SspMI CTAG 5 cut(s) 6, 89, 393, 399, 497
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 2 cut(s) 166, 343
TaiI ACGT 3 cut(s) 283, 553, 570
TaqI TCGA 3 cut(s) 69, 316, 453
TaqII GACCGA 1 cut(s) 201
TasI AATT 2 cut(s) 529, 595
TatI WGTACW 3 cut(s) 8, 51, 395
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 5 cut(s) 14, 82, 266, 387, 404
Tsp45I GTSAC 1 cut(s) 337
TspGWI ACGGA 2 cut(s) 50, 92
VpaK11BI GGWCC 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 453
XspI CTAG 5 cut(s) 6, 89, 393, 399, 497
ZrmI AGTACT 2 cut(s) 10, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.