MD12G1251900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010946
Physical Location & Seq
Forward (+)
32115352 .. 32118230
2879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1251900.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCTTCTTCTCTTGCCTTCCTTCAACCTCTCTCCAATGTCTCTTCCTCTTCAACCCTCCTCTTTCTCCGTAATCCCCACCATATCCCTTCTTCTTCCTCCTCCTTAAAACCCTCCAATTTCCCCAAAACCCTCCCATTCAAATCCCTCACCGCCTCATTCTCCCTCGCAGAATCAGACTCCCCCAAATCTTTCCAACCCAACGGTCCAAAATTTCAATCTTTCCTCCAAGAATTAGCCGACAGCTTTGATCTTCCACCGGACTACTTTGCGCAGCTTCCTAACGATCTCCGGCTCGATCTGAATGATGCTGCATTTGATCTTTCAAATGGAAAGATCGTCGACGAGTGTGGTCAAGAGTTAGGAGACACATTGTTAAATCTCTCTCGCGCATGGGAACAAGCTGATACATCAACTTCCCATGCTTTAGCTAGCAAGCTCCCTGGGTTGGAGGACTCTATGACAGGCAATGCCAAATCAGCATTTGGAAAGCGTTTGGTTTCTGCCGGAAGAAGATTTCAGTCTATGGGACAGTATGGCCAAGGTGAATTGCAAAAGATTGCAAAAGTAATGATTACAACCGGAAAGCTTCTGTCTGAAAGTTCAACATCGACAGTCACTGATGAACCAAAGACGGAAAGCAGGATGCTGAAGTTTGGAGAACTTCAGCTCGAGCTAACATCAGATAAAGCCACCATAGGGGCCGTAATCAGCTTGGCTTTTGGGATTCTTTCGTGGGAGCTAGCTCAGGGCATCCAAAACATACCGGAGAGTTCATTGCAGTACGCAAATGACAATGCTTTGATGCTGGCTAAGTCTTTGAGGGGCGCTCTGCTTACCATCTGCTATTCCTCAGCGATATTGTCTGCTTTTACTACTGTCGGACTTCTCTTACTCGGAAGACAATTAAAGTCACCAAACGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3082

Protein Analysis

310

Amino Acids

33.31

Weight (kDa)

5.71

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3082 PF11282 231 - 303 8.7e-06 Protein of unknown function (DUF3082)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 273
AccI GTMKAC 1 cut(s) 342
AccII CGCG 1 cut(s) 390
AciI CCGC 1 cut(s) 153
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 2 cut(s) 650, 671
AfaI GTAC 1 cut(s) 785
AfiI CCNNNNNNNGG 2 cut(s) 448, 699
AgsI TTSAA 6 cut(s) 26, 54, 142, 218, 327, 606
AjnI CCWGG 1 cut(s) 442
Alw26I GTCTC 2 cut(s) 46, 360
AlwNI CAGNNNCTG 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 671
AoxI GGCC 2 cut(s) 538, 702
ApeKI GCWGC 2 cut(s) 274, 311
ApoI RAATTY 1 cut(s) 212
AspLEI GCGC 3 cut(s) 274, 392, 830
AspS9I GGNCC 2 cut(s) 206, 702
AsuHPI GGTGA 3 cut(s) 142, 557, 906
AsuNHI GCTAGC 2 cut(s) 431, 742
AvaI CYCGRG 1 cut(s) 671
AvaII GGWCC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 907
BbvCI CCTCAGC 1 cut(s) 853
BbvI GCAGC 2 cut(s) 286, 298
BccI CCATC 1 cut(s) 848
BceAI ACGGC 1 cut(s) 689
BciT130I CCWGG 1 cut(s) 444
BcoDI GTCTC 2 cut(s) 46, 360
BfaI CTAG 2 cut(s) 432, 743
BfoI RGCGCY 1 cut(s) 831
BisI GCNGC 2 cut(s) 275, 312
BlsI GCNGC 2 cut(s) 276, 313
Bme1390I CCNGG 1 cut(s) 444
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 671
BmgT120I GGNCC 2 cut(s) 206, 702
BmiI GGNNCC 1 cut(s) 703
BmrFI CCNGG 1 cut(s) 444
BmsI GCATC 4 cut(s) 298, 636, 762, 795
BmtI GCTAGC 2 cut(s) 435, 746
BpiI GAAGAC 1 cut(s) 907
BplI GAGNNNNNCTC 2 cut(s) 814, 846
Bpu10I CCTNAGC 2 cut(s) 747, 853
BsaJI CCNNGG 3 cut(s) 442, 443, 541
BsaWI WCCGGW 3 cut(s) 259, 581, 766
Bsc4I CCNNNNNNNGG 2 cut(s) 448, 699
Bse3DI GCAATG 2 cut(s) 475, 776
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 442, 443, 541
BseGI GGATG 2 cut(s) 651, 753
BseLI CCNNNNNNNGG 2 cut(s) 448, 699
BseMI GCAATG 2 cut(s) 475, 776
BseMII CTCAG 2 cut(s) 761, 867
BseRI GAGGAG 2 cut(s) 50, 91
BseXI GCAGC 2 cut(s) 286, 298
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 2 cut(s) 540, 704
BsiHKCI CYCGRG 1 cut(s) 671
BsiSI CCGG 5 cut(s) 260, 292, 507, 582, 767
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 2 cut(s) 448, 699
BsmAI GTCTC 2 cut(s) 46, 360
BsmFI GGGAC 1 cut(s) 543
BsnI GGCC 2 cut(s) 540, 704
BsoBI CYCGRG 1 cut(s) 671
Bsp143I GATC 5 cut(s) 250, 286, 298, 319, 336
BspACI CCGC 1 cut(s) 153
BspANI GGCC 2 cut(s) 540, 704
BspCNI CTCAG 2 cut(s) 760, 866
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 1 cut(s) 703
BspOI GCTAGC 2 cut(s) 435, 746
BsrDI GCAATG 2 cut(s) 475, 776
BssECI CCNNGG 3 cut(s) 442, 443, 541
BssMI GATC 5 cut(s) 250, 286, 298, 319, 336
BssT1I CCWWGG 1 cut(s) 541
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 4 cut(s) 206, 534, 616, 880
Bst6I CTCTTC 2 cut(s) 49, 55
BstC8I GCNNGC 4 cut(s) 433, 437, 744, 810
BstDEI CTNAG 3 cut(s) 747, 813, 853
BstF5I GGATG 2 cut(s) 651, 753
BstFNI CGCG 1 cut(s) 390
BstH2I RGCGCY 1 cut(s) 831
BstHHI GCGC 3 cut(s) 274, 392, 830
BstKTI GATC 5 cut(s) 253, 289, 301, 322, 339
BstMAI GTCTC 2 cut(s) 46, 360
BstMBI GATC 5 cut(s) 250, 286, 298, 319, 336
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 444
BstSCI CCNGG 1 cut(s) 442
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 2 cut(s) 286, 298
BstV2I GAAGAC 1 cut(s) 907
BsuRI GGCC 2 cut(s) 540, 704
BtsCI GGATG 2 cut(s) 651, 753
BtsIMutI CAGTG 1 cut(s) 618
Cac8I GCNNGC 4 cut(s) 433, 437, 744, 810
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 3 cut(s) 274, 392, 830
Cfr13I GGNCC 2 cut(s) 206, 702
Csp6I GTAC 1 cut(s) 784
CviAII CATG 2 cut(s) 393, 422
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 3 cut(s) 747, 813, 853
DpnI GATC 5 cut(s) 252, 288, 300, 321, 338
DpnII GATC 5 cut(s) 250, 286, 298, 319, 336
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 2 cut(s) 49, 55
EarI CTCTTC 2 cut(s) 49, 55
Eco130I CCWWGG 1 cut(s) 541
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 650, 671
Eco88I CYCGRG 1 cut(s) 671
EcoRII CCWGG 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 541
FaeI CATG 2 cut(s) 396, 425
FaiI YATR 8 cut(s) 84, 394, 423, 461, 527, 537, 698, 764
FaqI GGGAC 1 cut(s) 543
FatI CATG 2 cut(s) 392, 421
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 2 cut(s) 275, 312
FokI GGATG 2 cut(s) 658, 740
Fsp4HI GCNGC 2 cut(s) 275, 312
FspBI CTAG 2 cut(s) 432, 743
FspI TGCGCA 1 cut(s) 273
GlaI GCGC 3 cut(s) 273, 391, 829
GluI GCNGC 2 cut(s) 275, 312
HaeII RGCGCY 1 cut(s) 831
HaeIII GGCC 2 cut(s) 540, 704
HapII CCGG 5 cut(s) 260, 292, 507, 582, 767
HhaI GCGC 3 cut(s) 274, 392, 830
Hin1II CATG 2 cut(s) 396, 425
Hin6I GCGC 3 cut(s) 272, 390, 828
HinP1I GCGC 3 cut(s) 272, 390, 828
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 4 cut(s) 173, 179, 455, 727
HpaII CCGG 5 cut(s) 260, 292, 507, 582, 767
HphI GGTGA 3 cut(s) 142, 557, 906
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 6 cut(s) 178, 303, 598, 685, 884, 899
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 2 cut(s) 344, 347
HpyAV CCTTC 3 cut(s) 28, 32, 99
HpyCH4III ACNGT 4 cut(s) 206, 534, 616, 880
HpyCH4V TGCA 4 cut(s) 314, 553, 563, 781
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 3 cut(s) 747, 813, 853
Hsp92II CATG 2 cut(s) 396, 425
HspAI GCGC 3 cut(s) 272, 390, 828
Kzo9I GATC 5 cut(s) 250, 286, 298, 319, 336
LmnI GCTCC 2 cut(s) 444, 739
Lsp1109I GCAGC 2 cut(s) 286, 298
LweI GCATC 4 cut(s) 298, 636, 762, 795
MaeI CTAG 2 cut(s) 432, 743
MaeIII GTNAC 2 cut(s) 616, 912
MalI GATC 5 cut(s) 252, 288, 300, 321, 338
MboI GATC 5 cut(s) 250, 286, 298, 319, 336
MboII GAAGA 8 cut(s) 36, 42, 84, 87, 245, 522, 525, 912
MlsI TGGCCA 1 cut(s) 540
MluCI AATT 5 cut(s) 118, 212, 233, 548, 905
MluNI TGGCCA 1 cut(s) 540
MlyI GAGTC 2 cut(s) 173, 449
MmeI TCCRAC 3 cut(s) 220, 429, 862
Mox20I TGGCCA 1 cut(s) 540
MscI TGGCCA 1 cut(s) 540
MseI TTAA 3 cut(s) 107, 377, 908
Msp20I TGGCCA 1 cut(s) 540
MspI CCGG 5 cut(s) 260, 292, 507, 582, 767
MspR9I CCNGG 1 cut(s) 444
MvaI CCWGG 1 cut(s) 444
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 479
NdeII GATC 5 cut(s) 250, 286, 298, 319, 336
NheI GCTAGC 2 cut(s) 431, 742
NlaIII CATG 2 cut(s) 396, 425
NlaIV GGNNCC 1 cut(s) 703
NmuCI GTSAC 2 cut(s) 616, 912
NsbI TGCGCA 1 cut(s) 273
PaeR7I CTCGAG 1 cut(s) 671
PasI CCCWGGG 1 cut(s) 443
PfeI GAWTC 2 cut(s) 173, 727
PkrI GCNGC 2 cut(s) 276, 313
PleI GAGTC 2 cut(s) 173, 449
PpsI GAGTC 2 cut(s) 173, 449
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 1 cut(s) 703
PspPI GGNCC 2 cut(s) 206, 702
PspXI VCTCGAGB 1 cut(s) 671
PstNI CAGNNNCTG 1 cut(s) 620
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
SalI GTCGAC 1 cut(s) 341
SaqAI TTAA 3 cut(s) 107, 377, 908
SatI GCNGC 2 cut(s) 275, 312
Sau3AI GATC 5 cut(s) 250, 286, 298, 319, 336
Sau96I GGNCC 2 cut(s) 206, 702
SchI GAGTC 2 cut(s) 173, 449
ScrFI CCNGG 1 cut(s) 444
SfaNI GCATC 4 cut(s) 298, 636, 762, 795
Sfr274I CTCGAG 1 cut(s) 671
SgrDI CGTCGACG 1 cut(s) 341
SinI GGWCC 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse9I AATT 5 cut(s) 118, 212, 233, 548, 905
SsiI CCGC 1 cut(s) 153
SspMI CTAG 2 cut(s) 432, 743
StyD4I CCNGG 1 cut(s) 442
StyI CCWWGG 1 cut(s) 541
TaaI ACNGT 4 cut(s) 206, 534, 616, 880
TaqI TCGA 4 cut(s) 297, 342, 611, 672
TasI AATT 5 cut(s) 118, 212, 233, 548, 905
TfiI GAWTC 2 cut(s) 173, 727
Tru1I TTAA 3 cut(s) 107, 377, 908
Tru9I TTAA 3 cut(s) 107, 377, 908
TscAI CASTG 1 cut(s) 625
TseFI GTSAC 2 cut(s) 616, 912
TseI GCWGC 2 cut(s) 274, 311
Tsp45I GTSAC 2 cut(s) 616, 912
TspDTI ATGAA 2 cut(s) 639, 765
TspGWI ACGGA 2 cut(s) 59, 650
TspRI CASTG 1 cut(s) 625
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 2 cut(s) 432, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.