Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006372

Physical Location & Seq

Genomic Coordinates Reverse (-)

32425074 .. 32429011

Gene Structure 3938 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1256700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGGCGGCTGAATCCATAGCAATGCTACCGTGGACAATATCCTCCGCCGTCCCCTCCGACCACCGCCTGCCGCCGCGCGGAGGATTACTCTGCGGTTCTCACATCCACACCTCCGCAGCTCCAGTTAGAAATTGCGTCGTCGCCAGATACTCCTCGACGCAATCTGCTCTGCTTTTCTCCAAGAACTCGCTCCGGAAATGCCTGCCGCCGCTAAAGGCGTTGGTGGCGTCGGAAACTCTGGACGCTCCGTCGACGCCGTTTAGTGGGGAGGATAAAGTCGGAGTCTTGCTGCTTAACCTCGGTGGGCCCGAGACCCTCGACGACGTCCAGCCCTTCTTGTTTAACCTCTTCGCTGATCCGGATATCATTAGACTGCCGAGGCTGTTTCGGTTCCTTCAGAAGCCATTGGCGCAATTTATATCCGTTCTGAGGGCGCCAAAGAGCAAAGAAGGCTACGCCTCCATTGGCGGCGGCTCTCCTCTTCGACGGATAACTGATGAACAGGCGGAAGAATTGAGGAAGGCCCTTTATGCGAAGGATGTCCCAGCAAAAGTGTATGTTGGTATGCGTTATTGGCATCCGTTTACTGAAGAAGCTATAGAACAGATAAAAAGAGATGGAATTACAAAGCTTGTCGTCCTTCCTCTTTATCCACAATTTTCAATATCAACTAGTGGTTCAAGCCTTCGGCTCCTGGAGAGTATATTCCGGGAGGACGAATATCTTGTCAACATGCAGCACACAGTAATACCATCTTGGTACCAGCGTGAAGGGTACATAACGGCAATGGCAAATTTGATTGAAAAAGAGCTTCAATGTTTTGACAGCCCTGAAAAAGTAATGATATTCTTTAGCGCACATGGAGTGCCACTTGCATATGTGGAAGAGGCTGGTGATCCGTACAAGGCGGAGATGGAGGAATGCATAGATTTGATAATGGAGGAGTTAGAAAAGAGAAAAATAACTAATGCGTACACCCTTGCCTATCAGAGCAGAGTTGGACCTGTGGAGTGGTTAAAACCCTACACAGATGAGACAATAATTGAGCTTGGAAAAAAGGGAGTCAAAAGACTGCTGGCAGTCCCTATAAGCTTTGTCAGCGAGCATATTGAAACTCTAGAAGAAATCGATGTTGAGTACAAAGAATTGGCTCTGAAATCTGGTATAGAGGTTTGGGGGCGTGTTCCCGCACTAGGATGTGAACCCACCTTCATTTCAGATTTGGCAGATGCGGTGATTGAGAGCTTGCCATATGTTGGAGCTATGGCAGTCTCACATCTTGAAGCTCGACAGTCTTTAGTTCCACTTGGGAGTGTGGAAGAGTTGTTAGCAACATATGATTCACAGCGTAGGGAGCTACCGGCACCTGTGACTGTTTGGGAATGGGGTTGGACAAAAAGTGCCGAGACTTGGAATGGAAGAGCAGCTATGTTGGCAGTGGTGGTTCTACTGGTCCTAGAAGTCACCACCGGGGAGGGGTTTTTGCACCAATGGGGCATATTACCCTTACATCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006725 GO:0006778 GO:0006779 GO:0006783 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009507 GO:0009536 GO:0009579 GO:0009987 GO:0016020 GO:0016829 GO:0018130 GO:0019438 GO:0031976 GO:0031984 GO:0033013 GO:0033014 GO:0034357 GO:0034641 GO:0042168 GO:0042440 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046148 GO:0046483 GO:0051186 GO:0051188 GO:0055035 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

Chloroa_b-bind Ferrochelatase

Protein Analysis

506

Amino Acids

56.33

Weight (kDa)

5.3

Isoelectric Point (pI)

50.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ferrochelatase	PF00762	92 - 415	3.1e-112	Ferrochelatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006372)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30390 AT2G30390
fragaria_vesca	FvH4_1g27860 FvH4_3g02572 FvH4_3g33000 FvH4_6g00970 FvH4_6g00970 FvH4_6g00970 FvH4_6g00970 FvH4_6g00970 FvH4_6g00970 FvH4_6g00970 FvH4_6g00970
malus_domestica	MD04G1239600.v1.1 MD12G1256700.v1.1
prunus_persica	Prupe.6G357500_v2.0.a1
pyrus_communis	pycom04g21160 pycom12g23540
rosa_chinensis	RchiOBHm_Chr3g0448431
rosa_multiflora	Rmu_co8294957.1_g000001 Rmu_co8484981.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426620
rosa_samantha	Rh3AG011800 Rh3BG011600 Rh3CG011300 Rh3DG012000
rosa_wichuraiana	Rw3G000910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	327
Acc65I	GGTACC	1 cut(s)	759
AccB1I	GGYRCC	3 cut(s)	433, 759, 1363
AccB7I	CCANNNNNTGG	1 cut(s)	1256
AccI	GTMKAC	1 cut(s)	251
AccII	CGCG	2 cut(s)	76, 78
AccIII	TCCGGA	2 cut(s)	192, 358
AclWI	GGATC	2 cut(s)	350, 890
AcsI	RAATTY	1 cut(s)	793
AcuI	CTGAAG	2 cut(s)	380, 609
AcyI	GRCGYC	4 cut(s)	227, 254, 324, 434
AfaI	GTAC	5 cut(s)	761, 776, 902, 974, 1139
AfiI	CCNNNNNNNGG	8 cut(s)	77, 80, 263, 429, 1193, 1256, 1410, 1476
AgsI	TTSAA	6 cut(s)	663, 681, 803, 815, 1112, 1283
AhdI	GACNNNNNGTC	1 cut(s)	247
AhlI	ACTAGT	1 cut(s)	671
AjnI	CCWGG	1 cut(s)	693
Alw26I	GTCTC	4 cut(s)	305, 1028, 1276, 1400
AlwI	GGATC	2 cut(s)	350, 890
Ama87I	CYCGRG	1 cut(s)	308
Aor13HI	TCCGGA	2 cut(s)	192, 358
AoxI	GGCC	2 cut(s)	305, 522
ApaI	GGGCCC	1 cut(s)	309
ApeKI	GCWGC	4 cut(s)	116, 289, 736, 1424
ApoI	RAATTY	1 cut(s)	793
ArsI	GACNNNNNNTTYG	2 cut(s)	711, 743
Asp718I	GGTACC	1 cut(s)	759
AspLEI	GCGC	4 cut(s)	78, 412, 436, 857
AspS9I	GGNCC	5 cut(s)	305, 306, 523, 1001, 1453
AsuC2I	CCSGG	2 cut(s)	710, 1471
AsuHPI	GGTGA	3 cut(s)	905, 1246, 1456
AvaI	CYCGRG	1 cut(s)	308
AvaII	GGWCC	2 cut(s)	1001, 1453
BaeGI	GKGCMC	1 cut(s)	309
BaeI	ACNNNNGTAYC	2 cut(s)	751, 784
BanI	GGYRCC	3 cut(s)	433, 759, 1363
BanII	GRGCYC	1 cut(s)	309
BbvI	GCAGC	4 cut(s)	128, 276, 748, 1436
BccI	CCATC	3 cut(s)	611, 760, 907
BceAI	ACGGC	3 cut(s)	32, 241, 798
BciT130I	CCWGG	1 cut(s)	695
BcnI	CCSGG	2 cut(s)	710, 1471
BcoDI	GTCTC	4 cut(s)	305, 1028, 1276, 1400
BcuI	ACTAGT	1 cut(s)	671
BfaI	CTAG	4 cut(s)	672, 1118, 1193, 1457
BfmI	CTRYAG	1 cut(s)	597
BfoI	RGCGCY	1 cut(s)	437
Bme1390I	CCNGG	3 cut(s)	695, 710, 1471
Bme18I	GGWCC	2 cut(s)	1001, 1453
BmeRI	GACNNNNNGTC	1 cut(s)	247
BmeT110I	CYCGRG	1 cut(s)	308
BmgT120I	GGNCC	5 cut(s)	305, 306, 523, 1001, 1453
BmiI	GGNNCC	6 cut(s)	307, 392, 435, 692, 761, 1365
BmrFI	CCNGG	3 cut(s)	695, 710, 1471
BmsI	GCATC	2 cut(s)	586, 1219
BplI	GAGNNNNNCTC	2 cut(s)	72, 104
BpmI	CTGGAG	2 cut(s)	105, 716
BpuMI	CCSGG	2 cut(s)	710, 1471
Bsa29I	ATCGAT	1 cut(s)	1128
BsaHI	GRCGYC	4 cut(s)	227, 254, 324, 434
BsaI	GGTCTC	1 cut(s)	305
BsaJI	CCNNGG	4 cut(s)	29, 298, 377, 1470
BsaWI	WCCGGW	2 cut(s)	192, 358
Bsc4I	CCNNNNNNNGG	8 cut(s)	77, 80, 263, 429, 1193, 1256, 1410, 1476
Bse118I	RCCGGY	1 cut(s)	1360
Bse1I	ACTGG	2 cut(s)	122, 1455
Bse3DI	GCAATG	2 cut(s)	27, 792
BseAI	TCCGGA	2 cut(s)	192, 358
BseBI	CCWGG	1 cut(s)	695
BseCI	ATCGAT	1 cut(s)	1128
BseDI	CCNNGG	4 cut(s)	29, 298, 377, 1470
BseGI	GGATG	4 cut(s)	102, 544, 577, 1202
BseLI	CCNNNNNNNGG	8 cut(s)	77, 80, 263, 429, 1193, 1256, 1410, 1476
BseMI	GCAATG	2 cut(s)	27, 792
BseMII	CTCAG	1 cut(s)	419
BseNI	ACTGG	2 cut(s)	122, 1455
BseRI	GAGGAG	3 cut(s)	142, 468, 956
BseSI	GKGCMC	1 cut(s)	309
BseXI	GCAGC	4 cut(s)	128, 276, 748, 1436
BseYI	CCCAGC	1 cut(s)	544
Bsh1236I	CGCG	2 cut(s)	76, 78
BshFI	GGCC	2 cut(s)	307, 524
BshNI	GGYRCC	3 cut(s)	433, 759, 1363
BshVI	ATCGAT	1 cut(s)	1128
BsiHKCI	CYCGRG	1 cut(s)	308
BsiSI	CCGG	5 cut(s)	193, 359, 709, 1361, 1470
BslFI	GGGAC	3 cut(s)	35, 527, 1067
BslI	CCNNNNNNNGG	8 cut(s)	77, 80, 263, 429, 1193, 1256, 1410, 1476
BsmAI	GTCTC	4 cut(s)	305, 1028, 1276, 1400
BsmFI	GGGAC	3 cut(s)	35, 527, 1067
BsmI	GAATGC	1 cut(s)	926
BsnI	GGCC	2 cut(s)	307, 524
Bso31I	GGTCTC	1 cut(s)	305
BsoBI	CYCGRG	1 cut(s)	308
Bsp120I	GGGCCC	1 cut(s)	305
Bsp1286I	GDGCHC	1 cut(s)	309
Bsp13I	TCCGGA	2 cut(s)	192, 358
Bsp143I	GATC	2 cut(s)	355, 895
BspANI	GGCC	2 cut(s)	307, 524
BspCNI	CTCAG	1 cut(s)	420
BspDI	ATCGAT	1 cut(s)	1128
BspEI	TCCGGA	2 cut(s)	192, 358
BspFNI	CGCG	2 cut(s)	76, 78
BspLI	GGNNCC	6 cut(s)	307, 392, 435, 692, 761, 1365
BspPI	GGATC	2 cut(s)	350, 890
BspQI	GCTCTTC	1 cut(s)	1414
BspT107I	GGYRCC	3 cut(s)	433, 759, 1363
BspTNI	GGTCTC	1 cut(s)	305
BsrDI	GCAATG	2 cut(s)	27, 792
BsrFI	RCCGGY	1 cut(s)	1360
BsrI	ACTGG	2 cut(s)	122, 1455
BssAI	RCCGGY	1 cut(s)	1360
BssECI	CCNNGG	4 cut(s)	29, 298, 377, 1470
BssMI	GATC	2 cut(s)	355, 895
BssNI	GRCGYC	4 cut(s)	227, 254, 324, 434
Bst2UI	CCWGG	1 cut(s)	695
Bst4CI	ACNGT	4 cut(s)	30, 745, 1293, 1375
Bst6I	CTCTTC	5 cut(s)	353, 486, 879, 1314, 1414
BstACI	GRCGYC	4 cut(s)	227, 254, 324, 434
BstC8I	GCNNGC	5 cut(s)	68, 203, 1077, 1103, 1247
BstDEI	CTNAG	1 cut(s)	428
BstDSI	CCRYGG	1 cut(s)	29
BstF5I	GGATG	4 cut(s)	102, 544, 577, 1202
BstFNI	CGCG	2 cut(s)	76, 78
BstH2I	RGCGCY	1 cut(s)	437
BstHHI	GCGC	4 cut(s)	78, 412, 436, 857
BstKTI	GATC	2 cut(s)	358, 898
BstMAI	GTCTC	4 cut(s)	305, 1028, 1276, 1400
BstMBI	GATC	2 cut(s)	355, 895
BstMWI	GCNNNNNNNGC	8 cut(s)	224, 409, 450, 530, 574, 1098, 1354, 1433
BstNI	CCWGG	1 cut(s)	695
BstNSI	RCATGY	1 cut(s)	736
BstSCI	CCNGG	3 cut(s)	693, 708, 1469
BstSFI	CTRYAG	1 cut(s)	597
BstSLI	GKGCMC	1 cut(s)	309
BstUI	CGCG	2 cut(s)	76, 78
BstV1I	GCAGC	4 cut(s)	128, 276, 748, 1436
Bsu15I	ATCGAT	1 cut(s)	1128
BsuRI	GGCC	2 cut(s)	307, 524
BsuTUI	ATCGAT	1 cut(s)	1128
BtgI	CCRYGG	1 cut(s)	29
BtgZI	GCGATG	1 cut(s)	1496
BtsCI	GGATG	4 cut(s)	102, 544, 577, 1202
BtsI	GCAGTG	1 cut(s)	1443
BtsIMutI	CAGTG	1 cut(s)	1443
Cac8I	GCNNGC	5 cut(s)	68, 203, 1077, 1103, 1247
CfoI	GCGC	4 cut(s)	78, 412, 436, 857
Cfr10I	RCCGGY	1 cut(s)	1360
Cfr13I	GGNCC	5 cut(s)	305, 306, 523, 1001, 1453
ClaI	ATCGAT	1 cut(s)	1128
CseI	GACGC	5 cut(s)	124, 166, 216, 251, 262
Csp6I	GTAC	5 cut(s)	760, 775, 901, 973, 1138
CviAII	CATG	2 cut(s)	733, 860
CviQI	GTAC	5 cut(s)	760, 775, 901, 973, 1138
DdeI	CTNAG	1 cut(s)	428
DinI	GGCGCC	1 cut(s)	435
DpnI	GATC	2 cut(s)	357, 897
DpnII	GATC	2 cut(s)	355, 895
DriI	GACNNNNNGTC	1 cut(s)	247
Eam1104I	CTCTTC	5 cut(s)	353, 486, 879, 1314, 1414
Eam1105I	GACNNNNNGTC	1 cut(s)	247
EarI	CTCTTC	5 cut(s)	353, 486, 879, 1314, 1414
EciI	GGCGGA	3 cut(s)	34, 521, 923
Eco24I	GRGCYC	1 cut(s)	309
Eco31I	GGTCTC	1 cut(s)	305
Eco32I	GATATC	1 cut(s)	364
Eco47I	GGWCC	2 cut(s)	1001, 1453
Eco57I	CTGAAG	2 cut(s)	380, 609
Eco88I	CYCGRG	1 cut(s)	308
EcoO109I	RGGNCCY	1 cut(s)	523
EcoRII	CCWGG	1 cut(s)	693
EcoRV	GATATC	1 cut(s)	364
EcoT22I	ATGCAT	1 cut(s)	926
EcoT38I	GRGCYC	1 cut(s)	309
EgeI	GGCGCC	1 cut(s)	435
EheI	GGCGCC	1 cut(s)	435
FaeI	CATG	2 cut(s)	736, 863
FaqI	GGGAC	3 cut(s)	35, 527, 1067
FatI	CATG	2 cut(s)	732, 859
FauI	CCCGC	1 cut(s)	1195
FauNDI	CATATG	3 cut(s)	877, 1252, 1336
FblI	GTMKAC	1 cut(s)	251
FokI	GGATG	4 cut(s)	89, 551, 564, 1209
FriOI	GRGCYC	1 cut(s)	309
FspBI	CTAG	4 cut(s)	672, 1118, 1193, 1457
GlaI	GCGC	4 cut(s)	77, 411, 435, 856
GsaI	CCCAGC	1 cut(s)	548
GsuI	CTGGAG	2 cut(s)	105, 716
HaeII	RGCGCY	1 cut(s)	437
HaeIII	GGCC	2 cut(s)	307, 524
HapII	CCGG	5 cut(s)	193, 359, 709, 1361, 1470
HgaI	GACGC	5 cut(s)	124, 166, 216, 251, 262
HhaI	GCGC	4 cut(s)	78, 412, 436, 857
Hin1I	GRCGYC	4 cut(s)	227, 254, 324, 434
Hin1II	CATG	2 cut(s)	736, 863
Hin6I	GCGC	4 cut(s)	76, 410, 434, 855
HinP1I	GCGC	4 cut(s)	76, 410, 434, 855
HincII	GTYRAC	2 cut(s)	252, 730
HindII	GTYRAC	2 cut(s)	252, 730
HindIII	AAGCTT	2 cut(s)	629, 1090
HinfI	GANTC	4 cut(s)	11, 282, 1062, 1340
HpaII	CCGG	5 cut(s)	193, 359, 709, 1361, 1470
HphI	GGTGA	3 cut(s)	905, 1246, 1456
Hpy166II	GTNNAC	6 cut(s)	33, 252, 585, 730, 975, 1202
Hpy188I	TCNGA	8 cut(s)	58, 232, 281, 399, 429, 990, 1155, 1219
Hpy188III	TCNNGA	5 cut(s)	193, 239, 359, 1118, 1280
Hpy8I	GTNNAC	6 cut(s)	33, 252, 585, 730, 975, 1202
Hpy99I	CGWCG	9 cut(s)	140, 143, 160, 232, 253, 256, 323, 326, 489
HpyAV	CCTTC	9 cut(s)	343, 404, 443, 514, 529, 650, 695, 764, 1219
HpyCH4III	ACNGT	4 cut(s)	30, 745, 1293, 1375
HpyCH4IV	ACGT	1 cut(s)	324
HpyCH4V	TGCA	4 cut(s)	736, 875, 924, 1486
HpyF10VI	GCNNNNNNNGC	8 cut(s)	224, 409, 450, 530, 574, 1098, 1354, 1433
HpyF3I	CTNAG	1 cut(s)	428
HpySE526I	ACGT	1 cut(s)	324
Hsp92I	GRCGYC	4 cut(s)	227, 254, 324, 434
Hsp92II	CATG	2 cut(s)	736, 863
HspAI	GCGC	4 cut(s)	76, 410, 434, 855
KasI	GGCGCC	1 cut(s)	433
Kpn2I	TCCGGA	2 cut(s)	192, 358
KpnI	GGTACC	1 cut(s)	763
Kzo9I	GATC	2 cut(s)	355, 895
LguI	GCTCTTC	1 cut(s)	1414
LmnI	GCTCC	6 cut(s)	124, 195, 250, 696, 1259, 1354
Lsp1109I	GCAGC	4 cut(s)	128, 276, 748, 1436
LweI	GCATC	2 cut(s)	586, 1219
MaeI	CTAG	4 cut(s)	672, 1118, 1193, 1457
MaeII	ACGT	1 cut(s)	324
MaeIII	GTNAC	2 cut(s)	1369, 1462
MalI	GATC	2 cut(s)	357, 897
MboI	GATC	2 cut(s)	355, 895
MboII	GAAGA	8 cut(s)	340, 473, 521, 602, 896, 1133, 1331, 1431
MhlI	GDGCHC	1 cut(s)	309
MluCI	AATT	8 cut(s)	130, 413, 512, 621, 656, 793, 1041, 1145
Mly113I	GGCGCC	1 cut(s)	434
MlyI	GAGTC	2 cut(s)	291, 1071
MmeI	TCCRAC	6 cut(s)	81, 210, 259, 979, 1237, 1370
Mph1103I	ATGCAT	1 cut(s)	926
MroI	TCCGGA	2 cut(s)	192, 358
MseI	TTAA	3 cut(s)	294, 342, 1016
MslI	CAYNNNNRTG	2 cut(s)	20, 1195
MspI	CCGG	5 cut(s)	193, 359, 709, 1361, 1470
MspR9I	CCNGG	3 cut(s)	695, 710, 1471
Mva1269I	GAATGC	1 cut(s)	926
MvaI	CCWGG	1 cut(s)	695
MvnI	CGCG	2 cut(s)	76, 78
MwoI	GCNNNNNNNGC	8 cut(s)	224, 409, 450, 530, 574, 1098, 1354, 1433
NarI	GGCGCC	1 cut(s)	434
NciI	CCSGG	2 cut(s)	710, 1471
NdeI	CATATG	3 cut(s)	877, 1252, 1336
NdeII	GATC	2 cut(s)	355, 895
NlaIII	CATG	2 cut(s)	736, 863
NlaIV	GGNNCC	6 cut(s)	307, 392, 435, 692, 761, 1365
NmeAIII	GCCGAG	2 cut(s)	402, 1429
NmuCI	GTSAC	2 cut(s)	1369, 1462
NsiI	ATGCAT	1 cut(s)	926
NspI	RCATGY	1 cut(s)	736
PciSI	GCTCTTC	1 cut(s)	1414
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PctI	GAATGC	1 cut(s)	926
PfeI	GAWTC	2 cut(s)	11, 1340
PflFI	GACNNNGTC	1 cut(s)	323
PflMI	CCANNNNNTGG	1 cut(s)	1256
PfoI	TCCNGGA	2 cut(s)	693, 708
PleI	GAGTC	2 cut(s)	290, 1070
PluTI	GGCGCC	1 cut(s)	437
PpsI	GAGTC	2 cut(s)	290, 1070
Psp6I	CCWGG	1 cut(s)	693
PspFI	CCCAGC	1 cut(s)	544
PspGI	CCWGG	1 cut(s)	693
PspN4I	GGNNCC	6 cut(s)	307, 392, 435, 692, 761, 1365
PspOMI	GGGCCC	1 cut(s)	305
PspPI	GGNCC	5 cut(s)	305, 306, 523, 1001, 1453
PsyI	GACNNNGTC	1 cut(s)	323
RsaI	GTAC	5 cut(s)	761, 776, 902, 974, 1139
RsaNI	GTAC	5 cut(s)	760, 775, 901, 973, 1138
RseI	CAYNNNNRTG	2 cut(s)	20, 1195
SalI	GTCGAC	1 cut(s)	250
SapI	GCTCTTC	1 cut(s)	1414
SaqAI	TTAA	3 cut(s)	294, 342, 1016
Sau3AI	GATC	2 cut(s)	355, 895
Sau96I	GGNCC	5 cut(s)	305, 306, 523, 1001, 1453
SchI	GAGTC	2 cut(s)	291, 1071
ScrFI	CCNGG	3 cut(s)	695, 710, 1471
SduI	GDGCHC	1 cut(s)	309
SfaNI	GCATC	2 cut(s)	586, 1219
SfcI	CTRYAG	1 cut(s)	597
SfoI	GGCGCC	1 cut(s)	435
SgrDI	CGTCGACG	1 cut(s)	250
SinI	GGWCC	2 cut(s)	1001, 1453
SmiMI	CAYNNNNRTG	2 cut(s)	20, 1195
SpeI	ACTAGT	1 cut(s)	671
Sse9I	AATT	8 cut(s)	130, 413, 512, 621, 656, 793, 1041, 1145
SspDI	GGCGCC	1 cut(s)	433
SspMI	CTAG	4 cut(s)	672, 1118, 1193, 1457
StyD4I	CCNGG	3 cut(s)	693, 708, 1469
TaaI	ACNGT	4 cut(s)	30, 745, 1293, 1375
TaiI	ACGT	1 cut(s)	327
TaqI	TCGA	6 cut(s)	155, 251, 318, 484, 1128, 1288
TasI	AATT	8 cut(s)	130, 413, 512, 621, 656, 793, 1041, 1145
TatI	WGTACW	1 cut(s)	1137
TauI	GCSGC	7 cut(s)	8, 73, 76, 208, 211, 471, 474
TfiI	GAWTC	2 cut(s)	11, 1340
Tru1I	TTAA	3 cut(s)	294, 342, 1016
Tru9I	TTAA	3 cut(s)	294, 342, 1016
TscAI	CASTG	1 cut(s)	1443
TseFI	GTSAC	2 cut(s)	1369, 1462
TseI	GCWGC	4 cut(s)	116, 289, 736, 1424
Tsp45I	GTSAC	2 cut(s)	1369, 1462
TspDTI	ATGAA	2 cut(s)	513, 1201
TspGWI	ACGGA	5 cut(s)	237, 412, 502, 570, 888
TspRI	CASTG	1 cut(s)	1443
Tth111I	GACNNNGTC	1 cut(s)	323
Van91I	CCANNNNNTGG	1 cut(s)	1256
VpaK11BI	GGWCC	2 cut(s)	1001, 1453
XapI	RAATTY	1 cut(s)	793
XbaI	TCTAGA	1 cut(s)	1117
XceI	RCATGY	1 cut(s)	736
XmiI	GTMKAC	1 cut(s)	251
XspI	CTAG	4 cut(s)	672, 1118, 1193, 1457
ZraI	GACGTC	1 cut(s)	325
Zsp2I	ATGCAT	1 cut(s)	926

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.