MD13G1000200.v1.1

Ferredoxin-thioredoxin reductase, variable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011069
Physical Location & Seq
Forward (+)
29481 .. 29984
504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1000200.v1.1.491

Sequence Viewer

Length: 495 bp
ATGAGTATCGCAACAGTGGCAGCCGCCGCCTCTTCATCCCTGAGCGCAGCAGCAGATGTGGGACAGATGAAATCGTTCTCATTTTCTTTCCCTTCTTGTTGGGCAGTTTCATTTTCACATCACAAACTCACTCCCACCCCTCCTGCTCACTGCCCACGGAGGAGGAAATTAAGTAGAAGGGTAATTTGCGAGGTCGCTGCCGCCGATGTATCTCCATCAACATCATCAGATGAAGCCACCGCCGCCGCCGCCTCCTCCAAGATCGGAGCTAGGGTTACAGTGAAGGTGCCTCTGAAGGTGTACCACGTGCCCAGAGTTCCGGAGGTAGACATTACTGGCATGGAAGGTGAGCTGAAGCAGTACGTTGGGCTGTGGAAGGGCAAGCGGATTTCAGCCAATCTCCCTTATAAGGTGCAGTTCGTCGTCGACGTTCAAGGCCGTGGCGCAGTTAAGTTCTTCGCCCATCTCAAGGAGGACGAGTTCGAATATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeThRed_A

Protein Analysis

165

Amino Acids

17.72

Weight (kDa)

9.48

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeThRed_A PF02941 88 - 160 6.5e-27 Ferredoxin thioredoxin reductase variable alpha chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08410 AT5G08410 AT5G23440
fragaria_vesca FvH4_4g37381 FvH4_4g37381
malus_domestica MD10G1109200.v1.1 MD13G1000200.v1.1 MD16G1005600.v1.1
prunus_persica Prupe.1G352600_v2.0.a1
pyrus_communis pycom13g00020 pycom2749g00070
rosa_chinensis RchiOBHm_Chr4g0447301
rosa_laevigata RLG00000005588
rosa_multiflora Rmu_ssc0000396.1_g000007
rosa_roxburghii Rroxscaffold_5G00387690
rosa_rugosa Rorug04G0379800 Rorug04G0379800
rosa_samantha Rh4BG450100 Rh4DG448500
rosa_wichuraiana Rw4G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
AccB1I GGYRCC 1 cut(s) 286
AccI GTMKAC 2 cut(s) 327, 426
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 8 cut(s) 24, 27, 201, 240, 243, 246, 249, 385
AcuI CTGAAG 2 cut(s) 314, 374
AcvI CACGTG 1 cut(s) 307
AfaI GTAC 2 cut(s) 302, 362
AfiI CCNNNNNNNGG 2 cut(s) 409, 469
AgsI TTSAA 1 cut(s) 434
AloI GAACNNNNNNTCC 1 cut(s) 464
AluBI AGCT 2 cut(s) 269, 352
AluI AGCT 2 cut(s) 269, 352
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 1 cut(s) 436
ApeKI GCWGC 4 cut(s) 20, 47, 50, 197
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 2 cut(s) 47, 446
AsuHPI GGTGA 1 cut(s) 359
AsuII TTCGAA 1 cut(s) 483
BaeGI GKGCMC 1 cut(s) 312
BanI GGYRCC 1 cut(s) 286
BbrPI CACGTG 1 cut(s) 307
BbvI GCAGC 4 cut(s) 32, 59, 62, 184
BccI CCATC 2 cut(s) 223, 471
BceAI ACGGC 1 cut(s) 423
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BfaI CTAG 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 452
BsaAI YACGTR 1 cut(s) 307
BsaJI CCNNGG 2 cut(s) 155, 439
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 464, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 409, 469
Bse1I ACTGG 1 cut(s) 340
BseAI TCCGGA 1 cut(s) 319
BseDI CCNNGG 2 cut(s) 155, 439
BseGI GGATG 1 cut(s) 35
BseLI CCNNNNNNNGG 2 cut(s) 409, 469
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 340
BseRI GAGGAG 2 cut(s) 175, 244
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 4 cut(s) 32, 59, 62, 184
BsgI GTGCAG 1 cut(s) 434
BshFI GGCC 1 cut(s) 438
BshNI GGYRCC 1 cut(s) 286
BsiSI CCGG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 409, 469
BsmFI GGGAC 1 cut(s) 75
BsnI GGCC 1 cut(s) 438
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 312
Bsp13I TCCGGA 1 cut(s) 319
Bsp143I GATC 1 cut(s) 261
BspACI CCGC 8 cut(s) 24, 27, 201, 240, 243, 246, 249, 385
BspANI GGCC 1 cut(s) 438
BspCNI CTCAG 1 cut(s) 33
BspEI TCCGGA 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 288
BspT104I TTCGAA 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 286
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 2 cut(s) 155, 439
BssMI GATC 1 cut(s) 261
Bst4CI ACNGT 2 cut(s) 16, 280
Bst6I CTCTTC 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 307
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 2 cut(s) 155, 439
BstF5I GGATG 1 cut(s) 35
BstHHI GCGC 2 cut(s) 47, 446
BstKTI GATC 1 cut(s) 264
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 4 cut(s) 17, 26, 242, 248
BstSLI GKGCMC 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 32, 59, 62, 184
BsuRI GGCC 1 cut(s) 438
BtgI CCRYGG 2 cut(s) 155, 439
BtsCI GGATG 1 cut(s) 35
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 3 cut(s) 21, 148, 285
Cac8I GCNNGC 1 cut(s) 383
CfoI GCGC 2 cut(s) 47, 446
Csp6I GTAC 2 cut(s) 301, 361
CviAII CATG 1 cut(s) 340
CviJI RGCY 7 cut(s) 23, 236, 269, 352, 370, 395, 438
CviKI_1 RGCY 7 cut(s) 23, 236, 269, 352, 370, 395, 438
CviQI GTAC 2 cut(s) 301, 361
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Eco57I CTGAAG 2 cut(s) 314, 374
Eco72I CACGTG 1 cut(s) 307
FaeI CATG 1 cut(s) 343
FaiI YATR 2 cut(s) 341, 408
FaqI GGGAC 1 cut(s) 75
FatI CATG 1 cut(s) 339
FblI GTMKAC 2 cut(s) 327, 426
FokI GGATG 1 cut(s) 22
FspBI CTAG 1 cut(s) 270
GlaI GCGC 2 cut(s) 46, 445
HaeIII GGCC 1 cut(s) 438
HapII CCGG 1 cut(s) 320
HhaI GCGC 2 cut(s) 47, 446
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 2 cut(s) 45, 444
HinP1I GCGC 2 cut(s) 45, 444
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HpaII CCGG 1 cut(s) 320
HphI GGTGA 1 cut(s) 359
Hpy166II GTNNAC 3 cut(s) 301, 328, 427
Hpy188I TCNGA 3 cut(s) 229, 266, 294
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 3 cut(s) 301, 328, 427
Hpy99I CGWCG 3 cut(s) 425, 428, 431
HpyAV CCTTC 6 cut(s) 102, 171, 277, 289, 338, 370
HpyCH4III ACNGT 2 cut(s) 16, 280
HpyCH4IV ACGT 3 cut(s) 306, 363, 429
HpyCH4V TGCA 1 cut(s) 415
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 26, 242, 248
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 3 cut(s) 306, 363, 429
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 2 cut(s) 45, 444
Kpn2I TCCGGA 1 cut(s) 319
Kzo9I GATC 1 cut(s) 261
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 5 cut(s) 53, 156, 321, 325, 333
Lsp1109I GCAGC 4 cut(s) 32, 59, 62, 184
MaeI CTAG 1 cut(s) 270
MaeII ACGT 3 cut(s) 306, 363, 429
MaeIII GTNAC 1 cut(s) 274
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 2 cut(s) 24, 448
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 2 cut(s) 167, 183
MroI TCCGGA 1 cut(s) 319
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 3 cut(s) 170, 450, 493
MspI CCGG 1 cut(s) 320
MwoI GCNNNNNNNGC 4 cut(s) 17, 26, 242, 248
NdeII GATC 1 cut(s) 261
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 1 cut(s) 288
NspV TTCGAA 1 cut(s) 483
PcsI WCGNNNNNNNCGW 2 cut(s) 201, 426
PdmI GAANNNNTTC 1 cut(s) 74
PmaCI CACGTG 1 cut(s) 307
PmlI CACGTG 1 cut(s) 307
Ppu21I YACGTR 1 cut(s) 307
PsiI TTATAA 1 cut(s) 408
PspCI CACGTG 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 288
RsaI GTAC 2 cut(s) 302, 362
RsaNI GTAC 2 cut(s) 301, 361
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 3 cut(s) 170, 450, 493
Sau3AI GATC 1 cut(s) 261
SduI GDGCHC 1 cut(s) 312
SfuI TTCGAA 1 cut(s) 483
SgrDI CGTCGACG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
Sse9I AATT 2 cut(s) 167, 183
SsiI CCGC 8 cut(s) 24, 27, 201, 240, 243, 246, 249, 385
SspMI CTAG 1 cut(s) 270
TaaI ACNGT 2 cut(s) 16, 280
TaiI ACGT 3 cut(s) 309, 366, 432
TaqI TCGA 2 cut(s) 426, 483
TasI AATT 2 cut(s) 167, 183
TauI GCSGC 6 cut(s) 26, 29, 203, 245, 248, 251
Tru1I TTAA 3 cut(s) 170, 450, 493
Tru9I TTAA 3 cut(s) 170, 450, 493
TscAI CASTG 3 cut(s) 21, 155, 285
TseI GCWGC 4 cut(s) 20, 47, 50, 197
TspDTI ATGAA 4 cut(s) 24, 83, 99, 246
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 3 cut(s) 21, 155, 285
XmiI GTMKAC 2 cut(s) 327, 426
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.