RLG00000005588

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011069
Physical Location & Seq
Reverse (-)
106380 .. 106904
525 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005588

Sequence Viewer

Length: 525 bp
ATGAGTATAGGAGGAGGAACACTGTTAAGCTCAGCCACAGCCACAGGCATAGGCAGAGCTGCCTCGAGTACACCAGCAGATGCGGGGCAAAGGAATTCACGATTCCCTTGTTGCTGGGCAGCGGCAGGGGCAAAGCTCACTCGCGCTCACTACCCAACAACTCTAACTCCAACTCCAACTCCAACTCCAACTCCAAGGAGGAAAAGTAGAATGATTGTTTGCGAAGTCGCTATCGGATCCGATTTATCTCCATCAACATCATCAGAGGTAGAAGCGGCCGCCTCTTCAAAGATCGGAGCTAGGGTCAAGGTAACCGTGCCTCTCAAGGTGTACCACGTGCCCAGAGTTCCCGAGGTCGACATCACTGGCATGGAAGGTGAACTCAAGCAGTACGTTGGCCTCTGGAAGGGCAAACGGATTTCTGCCAATCTCCCTTATAAAGTTCAGTTCTTTGTCGACGTCCAAGGCCGTGGCCCTGTTAAATTCTTTGCGCATCTCAAGGAGGACGAGTTCGAATACCTTTGA

Protein Analysis

175

Amino Acids

18.65

Weight (kDa)

9.6

Isoelectric Point (pI)

31.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeThRed_A PF02941 98 - 170 1e-27 Ferredoxin thioredoxin reductase variable alpha chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08410 AT5G08410 AT5G23440
fragaria_vesca FvH4_4g37381 FvH4_4g37381
malus_domestica MD10G1109200.v1.1 MD13G1000200.v1.1 MD16G1005600.v1.1
prunus_persica Prupe.1G352600_v2.0.a1
pyrus_communis pycom13g00020 pycom2749g00070
rosa_chinensis RchiOBHm_Chr4g0447301
rosa_laevigata RLG00000005588
rosa_multiflora Rmu_ssc0000396.1_g000007
rosa_roxburghii Rroxscaffold_5G00387690
rosa_rugosa Rorug04G0379800 Rorug04G0379800
rosa_samantha Rh4BG450100 Rh4DG448500
rosa_wichuraiana Rw4G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 438
AatII GACGTC 1 cut(s) 462
Acc16I TGCGCA 1 cut(s) 492
AccI GTMKAC 2 cut(s) 357, 456
AccII CGCG 1 cut(s) 144
AciI CCGC 4 cut(s) 83, 122, 275, 279
AclWI GGATC 2 cut(s) 231, 244
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 2 cut(s) 94, 482
AcvI CACGTG 1 cut(s) 337
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 3 cut(s) 70, 332, 392
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 1 cut(s) 288
AloI GAACNNNNNNTCC 1 cut(s) 494
AluBI AGCT 4 cut(s) 30, 59, 136, 299
AluI AGCT 4 cut(s) 30, 59, 136, 299
AlwI GGATC 2 cut(s) 231, 244
Ama87I CYCGRG 2 cut(s) 64, 350
AoxI GGCC 4 cut(s) 276, 397, 466, 472
ApeKI GCWGC 2 cut(s) 59, 119
ApoI RAATTY 2 cut(s) 94, 482
AspLEI GCGC 2 cut(s) 146, 493
AspS9I GGNCC 1 cut(s) 473
AsuHPI GGTGA 1 cut(s) 389
AsuII TTCGAA 1 cut(s) 513
AvaI CYCGRG 2 cut(s) 64, 350
BaeGI GKGCMC 1 cut(s) 342
BamHI GGATCC 1 cut(s) 236
BbrPI CACGTG 1 cut(s) 337
BbvI GCAGC 2 cut(s) 46, 131
BccI CCATC 1 cut(s) 259
BceAI ACGGC 1 cut(s) 453
BfaI CTAG 1 cut(s) 300
BisI GCNGC 5 cut(s) 60, 120, 123, 276, 279
BlpI GCTNAGC 1 cut(s) 31
BlsI GCNGC 5 cut(s) 61, 121, 124, 277, 280
BmeT110I CYCGRG 2 cut(s) 64, 350
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 238
BmsI GCATC 2 cut(s) 70, 502
Bpu1102I GCTNAGC 1 cut(s) 31
Bpu14I TTCGAA 1 cut(s) 513
BpuEI CTTGAG 3 cut(s) 308, 368, 482
BsaAI YACGTR 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 4 cut(s) 194, 351, 463, 469
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse1I ACTGG 1 cut(s) 370
BseDI CCNNGG 4 cut(s) 194, 351, 463, 469
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 342
BseX3I CGGCCG 1 cut(s) 276
BseXI GCAGC 2 cut(s) 46, 131
BseYI CCCAGC 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 4 cut(s) 278, 399, 468, 474
BsiEI CGRYCG 1 cut(s) 279
BsiHKCI CYCGRG 2 cut(s) 64, 350
BslI CCNNNNNNNGG 1 cut(s) 406
BsnI GGCC 4 cut(s) 278, 399, 468, 474
BsoBI CYCGRG 2 cut(s) 64, 350
Bsp119I TTCGAA 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 2 cut(s) 236, 291
Bsp1720I GCTNAGC 1 cut(s) 31
BspACI CCGC 4 cut(s) 83, 122, 275, 279
BspANI GGCC 4 cut(s) 278, 399, 468, 474
BspCNI CTCAG 1 cut(s) 44
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 238
BspPI GGATC 2 cut(s) 231, 244
BspT104I TTCGAA 1 cut(s) 513
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 4 cut(s) 194, 351, 463, 469
BssMI GATC 2 cut(s) 236, 291
BssNI GRCGYC 1 cut(s) 459
BssT1I CCWWGG 2 cut(s) 194, 463
Bst4CI ACNGT 2 cut(s) 24, 316
Bst6I CTCTTC 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 513
BstDEI CTNAG 1 cut(s) 31
BstDSI CCRYGG 1 cut(s) 469
BstEII GGTNACC 1 cut(s) 310
BstENI CCTNNNNNAGG 1 cut(s) 404
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 2 cut(s) 146, 493
BstKTI GATC 2 cut(s) 239, 294
BstMBI GATC 2 cut(s) 236, 291
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstPI GGTNACC 1 cut(s) 310
BstSLI GKGCMC 1 cut(s) 342
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 46, 131
BstX2I RGATCY 1 cut(s) 236
BstXI CCANNNNNNTGG 1 cut(s) 470
BstYI RGATCY 1 cut(s) 236
BstZI CGGCCG 1 cut(s) 276
BsuRI GGCC 4 cut(s) 278, 399, 468, 474
BtgI CCRYGG 1 cut(s) 469
BtsIMutI CAGTG 2 cut(s) 20, 363
CciNI GCGGCCGC 1 cut(s) 276
CfoI GCGC 2 cut(s) 146, 493
Cfr13I GGNCC 1 cut(s) 473
Csp6I GTAC 3 cut(s) 69, 331, 391
CviAII CATG 1 cut(s) 370
CviQI GTAC 3 cut(s) 69, 331, 391
DdeI CTNAG 1 cut(s) 31
DpnI GATC 2 cut(s) 238, 293
DpnII GATC 2 cut(s) 236, 291
EaeI YGGCCR 1 cut(s) 276
EagI CGGCCG 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 276
Eco130I CCWWGG 2 cut(s) 194, 463
Eco52I CGGCCG 1 cut(s) 276
Eco72I CACGTG 1 cut(s) 337
Eco88I CYCGRG 2 cut(s) 64, 350
Eco91I GGTNACC 1 cut(s) 310
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 94
EcoT14I CCWWGG 2 cut(s) 194, 463
ErhI CCWWGG 2 cut(s) 194, 463
FaeI CATG 1 cut(s) 373
FaiI YATR 4 cut(s) 8, 50, 371, 438
FatI CATG 1 cut(s) 369
FauI CCCGC 1 cut(s) 76
FblI GTMKAC 2 cut(s) 357, 456
Fnu4HI GCNGC 5 cut(s) 60, 120, 123, 276, 279
Fsp4HI GCNGC 5 cut(s) 60, 120, 123, 276, 279
FspBI CTAG 1 cut(s) 300
FspI TGCGCA 1 cut(s) 492
GlaI GCGC 2 cut(s) 145, 492
GluI GCNGC 5 cut(s) 60, 120, 123, 276, 279
GsaI CCCAGC 1 cut(s) 118
HaeIII GGCC 4 cut(s) 278, 399, 468, 474
HhaI GCGC 2 cut(s) 146, 493
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 2 cut(s) 144, 491
HinP1I GCGC 2 cut(s) 144, 491
HincII GTYRAC 2 cut(s) 358, 457
HindII GTYRAC 2 cut(s) 358, 457
HinfI GANTC 1 cut(s) 102
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 5 cut(s) 71, 331, 358, 380, 457
Hpy188I TCNGA 4 cut(s) 236, 241, 265, 296
Hpy188III TCNNGA 3 cut(s) 99, 350, 403
Hpy8I GTNNAC 5 cut(s) 71, 331, 358, 380, 457
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 2 cut(s) 368, 400
HpyCH4III ACNGT 2 cut(s) 24, 316
HpyCH4IV ACGT 3 cut(s) 336, 393, 459
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 1 cut(s) 31
HpySE526I ACGT 3 cut(s) 336, 393, 459
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 2 cut(s) 144, 491
Kzo9I GATC 2 cut(s) 236, 291
LmnI GCTCC 1 cut(s) 296
LpnPI CCDG 8 cut(s) 30, 87, 100, 111, 351, 355, 388, 489
Lsp1109I GCAGC 2 cut(s) 46, 131
LweI GCATC 2 cut(s) 70, 502
MaeI CTAG 1 cut(s) 300
MaeII ACGT 3 cut(s) 336, 393, 459
MaeIII GTNAC 1 cut(s) 310
MalI GATC 2 cut(s) 238, 293
MboI GATC 2 cut(s) 236, 291
MboII GAAGA 1 cut(s) 276
MflI RGATCY 1 cut(s) 236
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 2 cut(s) 94, 482
MmeI TCCRAC 4 cut(s) 194, 200, 206, 212
MseI TTAA 2 cut(s) 26, 480
MslI CAYNNNNRTG 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 122
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 128
NdeII GATC 2 cut(s) 236, 291
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 1 cut(s) 238
NotI GCGGCCGC 1 cut(s) 276
NsbI TGCGCA 1 cut(s) 492
NspV TTCGAA 1 cut(s) 513
PaeR7I CTCGAG 1 cut(s) 64
PfeI GAWTC 1 cut(s) 102
PkrI GCNGC 5 cut(s) 61, 121, 124, 277, 280
PmaCI CACGTG 1 cut(s) 337
PmlI CACGTG 1 cut(s) 337
Ppu21I YACGTR 1 cut(s) 337
PsiI TTATAA 1 cut(s) 438
PspCI CACGTG 1 cut(s) 337
PspEI GGTNACC 1 cut(s) 310
PspFI CCCAGC 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 1 cut(s) 473
PspXI VCTCGAGB 1 cut(s) 64
PsuI RGATCY 1 cut(s) 236
RsaI GTAC 3 cut(s) 70, 332, 392
RsaNI GTAC 3 cut(s) 69, 331, 391
RseI CAYNNNNRTG 1 cut(s) 368
SalI GTCGAC 2 cut(s) 356, 455
SaqAI TTAA 2 cut(s) 26, 480
SatI GCNGC 5 cut(s) 60, 120, 123, 276, 279
Sau3AI GATC 2 cut(s) 236, 291
Sau96I GGNCC 1 cut(s) 473
SduI GDGCHC 1 cut(s) 342
SfaNI GCATC 2 cut(s) 70, 502
Sfr274I CTCGAG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 513
SlaI CTCGAG 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 4 cut(s) 64, 323, 383, 497
SmoI CTYRAG 4 cut(s) 64, 323, 383, 497
Sse9I AATT 2 cut(s) 94, 482
SsiI CCGC 4 cut(s) 83, 122, 275, 279
SspMI CTAG 1 cut(s) 300
StyI CCWWGG 2 cut(s) 194, 463
TaaI ACNGT 2 cut(s) 24, 316
TaiI ACGT 3 cut(s) 339, 396, 462
TaqI TCGA 4 cut(s) 65, 357, 456, 513
TasI AATT 2 cut(s) 94, 482
TatI WGTACW 1 cut(s) 68
TauI GCSGC 3 cut(s) 125, 278, 281
TfiI GAWTC 1 cut(s) 102
Tru1I TTAA 2 cut(s) 26, 480
Tru9I TTAA 2 cut(s) 26, 480
TscAI CASTG 2 cut(s) 27, 370
TseI GCWGC 2 cut(s) 59, 119
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 2 cut(s) 27, 370
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 2 cut(s) 94, 482
XhoI CTCGAG 1 cut(s) 64
XmiI GTMKAC 2 cut(s) 357, 456
XspI CTAG 1 cut(s) 300
ZraI GACGTC 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.