Rh4DG448500

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011069
Physical Location & Seq
Forward (+)
64764660 .. 64766834
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG448500.1

Sequence Viewer

Length: 525 bp
ATGAGTATAGGAGGAGGAACAGTAGGGTTATTAAGCTCAGCCACAGCCACAGCCACAGCCATAGGCAGAGCTGCCTCGAGTACACCAGCTGATGCGGGGCAAAGGAATTCACGATTCCCTTGTTGCTGGGCAGCGGCAGGGGCAAAGCTCACTCGCGCACACTACCCAACAACTCTAAATCCAACTCCAACTCCAAGGAGGAAAAGTAGAAGGATTGTTTGCGAAGTCGCTATCGGATCCGATTTATCTCCATCAACATCATCAGAGGTAGAAGCGGCCGCCTCTTCAAAGATCGGGGCTAGGGTCAAGGTAACCGTGCCTCTCAAGGTGTACCACGTACCCAGAGTTCCCGAGGTCGACATCACTGGCATGGAAGGTGAACTCAAGCAGTACGTTGGCCTCTGGAAGGGCAAACGGATTTCTGCCAATCTCCCTTATAAAGTACAGTTCTTTGTCGACGTCGAAGGCCGTGGCCCTGTTAAATTCTTTGCGCATCTCAAGGAGGACGAGTTCGAATACCTTTGA

Protein Analysis

174

Amino Acids

18.64

Weight (kDa)

9.6

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeThRed_A PF02941 98 - 170 1e-27 Ferredoxin thioredoxin reductase variable alpha chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08410 AT5G08410 AT5G23440
fragaria_vesca FvH4_4g37381 FvH4_4g37381
malus_domestica MD10G1109200.v1.1 MD13G1000200.v1.1 MD16G1005600.v1.1
prunus_persica Prupe.1G352600_v2.0.a1
pyrus_communis pycom13g00020 pycom2749g00070
rosa_chinensis RchiOBHm_Chr4g0447301
rosa_laevigata RLG00000005588
rosa_multiflora Rmu_ssc0000396.1_g000007
rosa_roxburghii Rroxscaffold_5G00387690
rosa_rugosa Rorug04G0379800 Rorug04G0379800
rosa_samantha Rh4BG450100 Rh4DG448500
rosa_wichuraiana Rw4G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 438
AatII GACGTC 1 cut(s) 462
Acc16I TGCGCA 1 cut(s) 492
AccI GTMKAC 2 cut(s) 357, 456
AccII CGCG 1 cut(s) 156
AciI CCGC 4 cut(s) 95, 134, 275, 279
AclWI GGATC 2 cut(s) 231, 244
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 2 cut(s) 106, 482
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 5 cut(s) 82, 332, 339, 392, 444
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 1 cut(s) 288
AloI GAACNNNNNNTCC 1 cut(s) 494
AluBI AGCT 4 cut(s) 36, 71, 89, 148
AluI AGCT 4 cut(s) 36, 71, 89, 148
AlwI GGATC 2 cut(s) 231, 244
Ama87I CYCGRG 2 cut(s) 76, 350
AoxI GGCC 4 cut(s) 276, 397, 466, 472
ApeKI GCWGC 2 cut(s) 71, 131
ApoI RAATTY 2 cut(s) 106, 482
AspLEI GCGC 2 cut(s) 158, 493
AspS9I GGNCC 1 cut(s) 473
AsuHPI GGTGA 1 cut(s) 389
AsuII TTCGAA 1 cut(s) 513
AvaI CYCGRG 2 cut(s) 76, 350
BamHI GGATCC 1 cut(s) 236
BbvI GCAGC 2 cut(s) 58, 143
BccI CCATC 1 cut(s) 259
BceAI ACGGC 1 cut(s) 453
BfaI CTAG 1 cut(s) 300
BisI GCNGC 5 cut(s) 72, 132, 135, 276, 279
BlpI GCTNAGC 1 cut(s) 37
BlsI GCNGC 5 cut(s) 73, 133, 136, 277, 280
BmeT110I CYCGRG 2 cut(s) 76, 350
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 238
BmsI GCATC 2 cut(s) 82, 502
Bpu1102I GCTNAGC 1 cut(s) 37
Bpu14I TTCGAA 1 cut(s) 513
BpuEI CTTGAG 3 cut(s) 308, 368, 482
BsaAI YACGTR 1 cut(s) 337
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 3 cut(s) 194, 351, 469
BsaXI ACNNNNNCTCC 8 cut(s) 6, 36, 175, 190, 205, 220, 494, 524
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse1I ACTGG 1 cut(s) 370
BseDI CCNNGG 3 cut(s) 194, 351, 469
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 27
BseX3I CGGCCG 1 cut(s) 276
BseXI GCAGC 2 cut(s) 58, 143
BseYI CCCAGC 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 4 cut(s) 278, 399, 468, 474
BsiEI CGRYCG 1 cut(s) 279
BsiHKCI CYCGRG 2 cut(s) 76, 350
BslI CCNNNNNNNGG 1 cut(s) 406
BsnI GGCC 4 cut(s) 278, 399, 468, 474
BsoBI CYCGRG 2 cut(s) 76, 350
Bsp119I TTCGAA 1 cut(s) 513
Bsp143I GATC 2 cut(s) 236, 291
Bsp1720I GCTNAGC 1 cut(s) 37
BspACI CCGC 4 cut(s) 95, 134, 275, 279
BspANI GGCC 4 cut(s) 278, 399, 468, 474
BspCNI CTCAG 1 cut(s) 50
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 238
BspPI GGATC 2 cut(s) 231, 244
BspT104I TTCGAA 1 cut(s) 513
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 3 cut(s) 194, 351, 469
BssMI GATC 2 cut(s) 236, 291
BssNI GRCGYC 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 194
Bst4CI ACNGT 3 cut(s) 22, 316, 447
Bst6I CTCTTC 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 513
BstDEI CTNAG 1 cut(s) 37
BstDSI CCRYGG 1 cut(s) 469
BstEII GGTNACC 1 cut(s) 310
BstENI CCTNNNNNAGG 1 cut(s) 404
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 2 cut(s) 158, 493
BstKTI GATC 2 cut(s) 239, 294
BstMBI GATC 2 cut(s) 236, 291
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 310
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 58, 143
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BstZI CGGCCG 1 cut(s) 276
BsuRI GGCC 4 cut(s) 278, 399, 468, 474
BtgI CCRYGG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 363
CciNI GCGGCCGC 1 cut(s) 276
CfoI GCGC 2 cut(s) 158, 493
Cfr13I GGNCC 1 cut(s) 473
Csp6I GTAC 5 cut(s) 81, 331, 338, 391, 443
CviAII CATG 1 cut(s) 370
CviQI GTAC 5 cut(s) 81, 331, 338, 391, 443
DdeI CTNAG 1 cut(s) 37
DpnI GATC 2 cut(s) 238, 293
DpnII GATC 2 cut(s) 236, 291
EaeI YGGCCR 1 cut(s) 276
EagI CGGCCG 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 194
Eco52I CGGCCG 1 cut(s) 276
Eco88I CYCGRG 2 cut(s) 76, 350
Eco91I GGTNACC 1 cut(s) 310
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 1 cut(s) 373
FaiI YATR 4 cut(s) 8, 62, 371, 438
FatI CATG 1 cut(s) 369
FauI CCCGC 1 cut(s) 88
FblI GTMKAC 2 cut(s) 357, 456
Fnu4HI GCNGC 5 cut(s) 72, 132, 135, 276, 279
Fsp4HI GCNGC 5 cut(s) 72, 132, 135, 276, 279
FspBI CTAG 1 cut(s) 300
FspI TGCGCA 1 cut(s) 492
GlaI GCGC 2 cut(s) 157, 492
GluI GCNGC 5 cut(s) 72, 132, 135, 276, 279
GsaI CCCAGC 1 cut(s) 130
HaeIII GGCC 4 cut(s) 278, 399, 468, 474
HhaI GCGC 2 cut(s) 158, 493
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 2 cut(s) 156, 491
HinP1I GCGC 2 cut(s) 156, 491
HincII GTYRAC 2 cut(s) 358, 457
HindII GTYRAC 2 cut(s) 358, 457
HinfI GANTC 1 cut(s) 114
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 5 cut(s) 83, 331, 358, 380, 457
Hpy188I TCNGA 3 cut(s) 236, 241, 265
Hpy188III TCNNGA 3 cut(s) 111, 350, 403
Hpy8I GTNNAC 5 cut(s) 83, 331, 358, 380, 457
Hpy99I CGWCG 2 cut(s) 461, 464
HpyAV CCTTC 4 cut(s) 204, 368, 400, 458
HpyCH4III ACNGT 3 cut(s) 22, 316, 447
HpyCH4IV ACGT 3 cut(s) 336, 393, 459
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 3 cut(s) 336, 393, 459
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 2 cut(s) 156, 491
Kzo9I GATC 2 cut(s) 236, 291
LpnPI CCDG 7 cut(s) 99, 112, 123, 351, 355, 388, 489
Lsp1109I GCAGC 2 cut(s) 58, 143
LweI GCATC 2 cut(s) 82, 502
MaeI CTAG 1 cut(s) 300
MaeII ACGT 3 cut(s) 336, 393, 459
MaeIII GTNAC 1 cut(s) 310
MalI GATC 2 cut(s) 238, 293
MboI GATC 2 cut(s) 236, 291
MboII GAAGA 1 cut(s) 276
MflI RGATCY 1 cut(s) 236
MluCI AATT 2 cut(s) 106, 482
MmeI TCCRAC 2 cut(s) 206, 212
MseI TTAA 2 cut(s) 32, 480
MslI CAYNNNNRTG 1 cut(s) 368
MspA1I CMGCKG 2 cut(s) 89, 134
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 2 cut(s) 236, 291
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 1 cut(s) 238
NotI GCGGCCGC 1 cut(s) 276
NsbI TGCGCA 1 cut(s) 492
NspV TTCGAA 1 cut(s) 513
PaeR7I CTCGAG 1 cut(s) 76
PfeI GAWTC 1 cut(s) 114
PkrI GCNGC 5 cut(s) 73, 133, 136, 277, 280
Ppu21I YACGTR 1 cut(s) 337
PsiI TTATAA 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 310
PspFI CCCAGC 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 1 cut(s) 473
PspXI VCTCGAGB 1 cut(s) 76
PsrI GAACNNNNNNTAC 2 cut(s) 330, 362
PsuI RGATCY 1 cut(s) 236
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 5 cut(s) 82, 332, 339, 392, 444
RsaNI GTAC 5 cut(s) 81, 331, 338, 391, 443
RseI CAYNNNNRTG 1 cut(s) 368
SalI GTCGAC 2 cut(s) 356, 455
SaqAI TTAA 2 cut(s) 32, 480
SatI GCNGC 5 cut(s) 72, 132, 135, 276, 279
Sau3AI GATC 2 cut(s) 236, 291
Sau96I GGNCC 1 cut(s) 473
SfaNI GCATC 2 cut(s) 82, 502
Sfr274I CTCGAG 1 cut(s) 76
SfuI TTCGAA 1 cut(s) 513
SlaI CTCGAG 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 4 cut(s) 76, 323, 383, 497
SmoI CTYRAG 4 cut(s) 76, 323, 383, 497
Sse9I AATT 2 cut(s) 106, 482
SsiI CCGC 4 cut(s) 95, 134, 275, 279
SspMI CTAG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 3 cut(s) 22, 316, 447
TaiI ACGT 3 cut(s) 339, 396, 462
TaqI TCGA 5 cut(s) 77, 357, 456, 462, 513
TasI AATT 2 cut(s) 106, 482
TatI WGTACW 2 cut(s) 80, 442
TauI GCSGC 3 cut(s) 137, 278, 281
TfiI GAWTC 1 cut(s) 114
Tru1I TTAA 2 cut(s) 32, 480
Tru9I TTAA 2 cut(s) 32, 480
TscAI CASTG 1 cut(s) 370
TseI GCWGC 2 cut(s) 71, 131
TspGWI ACGGA 1 cut(s) 430
TspRI CASTG 1 cut(s) 370
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 2 cut(s) 106, 482
XhoI CTCGAG 1 cut(s) 76
XmiI GTMKAC 2 cut(s) 357, 456
XspI CTAG 1 cut(s) 300
ZraI GACGTC 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.