pycom13g00020

Ferredoxin-thioredoxin reductase, variable

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011069
Physical Location & Seq
Forward (+)
14479 .. 14988
510 bp
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UTR
Exon/CDS
Intron
pycom13g00020.1

Sequence Viewer

Length: 510 bp
ATGGACACAGGAGCAATGAGTATCGCAACAGTGGCAGCCGCCGCCTCTTCATCCCTGAGCGCAGTAGCAGATGTGGGACAGATGAAATCGTTCTCATTTTCTTTCCCTTCTTGTTGGGCAGTTTCTTTTTCACATCACAAACTCACTCCCACCCCTCCTGCCCACTGCCCGCGGAGGAGGAAATTAAGTAGAAGGGTAATTTGCGAGGTCGCTGCCGCCGATGTATCTCCATCAACATCATCAGATGAAGCCACCGCCGCCGCAGCCTCCTCCAAGATCGGATCTAGGGTTACAGTGAAGGTGCCTCTGAAGGTGTACCACGTGCCCAGAGTTCCGGAGGTAGACATTACTGGCATGGAAGGTGAGCTGAAGCAGTACGTTGGGCTGTGGAAGGGCAAGCGGATTTCAGCCAATCTCCCTTATAAGGTGCAGTTCGTCGTCGACGTTCAAGGCCGTGGCGCAGTTAAGTTCTTTGCCCATCTCAAGGAGGACGAGTTCGAATATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeThRed_A

Protein Analysis

170

Amino Acids

18.24

Weight (kDa)

9.35

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeThRed_A PF02941 93 - 165 7e-27 Ferredoxin thioredoxin reductase variable alpha chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08410 AT5G08410 AT5G23440
fragaria_vesca FvH4_4g37381 FvH4_4g37381
malus_domestica MD10G1109200.v1.1 MD13G1000200.v1.1 MD16G1005600.v1.1
prunus_persica Prupe.1G352600_v2.0.a1
pyrus_communis pycom13g00020 pycom2749g00070
rosa_chinensis RchiOBHm_Chr4g0447301
rosa_laevigata RLG00000005588
rosa_multiflora Rmu_ssc0000396.1_g000007
rosa_roxburghii Rroxscaffold_5G00387690
rosa_rugosa Rorug04G0379800 Rorug04G0379800
rosa_samantha Rh4BG450100 Rh4DG448500
rosa_wichuraiana Rw4G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 301
AccI GTMKAC 2 cut(s) 342, 441
AccII CGCG 1 cut(s) 172
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 9 cut(s) 39, 42, 170, 172, 216, 255, 258, 261, 400
AclWI GGATC 1 cut(s) 289
AcuI CTGAAG 2 cut(s) 329, 389
AcvI CACGTG 1 cut(s) 322
AfaI GTAC 2 cut(s) 317, 377
AfiI CCNNNNNNNGG 2 cut(s) 424, 484
AgsI TTSAA 1 cut(s) 449
AloI GAACNNNNNNTCC 1 cut(s) 479
AluBI AGCT 1 cut(s) 367
AluI AGCT 1 cut(s) 367
AlwI GGATC 1 cut(s) 289
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 1 cut(s) 451
ApeKI GCWGC 3 cut(s) 35, 212, 263
Asp700I GAANNNNTTC 1 cut(s) 89
AspLEI GCGC 2 cut(s) 62, 461
AsuHPI GGTGA 1 cut(s) 374
AsuII TTCGAA 1 cut(s) 498
BaeGI GKGCMC 1 cut(s) 327
BanI GGYRCC 1 cut(s) 301
BbrPI CACGTG 1 cut(s) 322
BbvI GCAGC 3 cut(s) 47, 199, 275
BccI CCATC 2 cut(s) 238, 486
BceAI ACGGC 1 cut(s) 438
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BfaI CTAG 1 cut(s) 285
BisI GCNGC 8 cut(s) 36, 39, 42, 213, 216, 258, 261, 264
BlsI GCNGC 8 cut(s) 37, 40, 43, 214, 217, 259, 262, 265
BmiI GGNNCC 1 cut(s) 303
Bpu10I CCTNAGC 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 467
BsaAI YACGTR 1 cut(s) 322
BsaJI CCNNGG 2 cut(s) 170, 454
BsaWI WCCGGW 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 484
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 21
BseAI TCCGGA 1 cut(s) 334
BseDI CCNNGG 2 cut(s) 170, 454
BseGI GGATG 1 cut(s) 50
BseLI CCNNNNNNNGG 2 cut(s) 424, 484
BseMI GCAATG 1 cut(s) 21
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 2 cut(s) 190, 259
BseSI GKGCMC 1 cut(s) 327
BseXI GCAGC 3 cut(s) 47, 199, 275
BsgI GTGCAG 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 1 cut(s) 453
BshNI GGYRCC 1 cut(s) 301
BsiSI CCGG 1 cut(s) 335
BslFI GGGAC 1 cut(s) 90
BslI CCNNNNNNNGG 2 cut(s) 424, 484
BsmFI GGGAC 1 cut(s) 90
BsnI GGCC 1 cut(s) 453
Bsp119I TTCGAA 1 cut(s) 498
Bsp1286I GDGCHC 1 cut(s) 327
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 2 cut(s) 276, 281
BspACI CCGC 9 cut(s) 39, 42, 170, 172, 216, 255, 258, 261, 400
BspANI GGCC 1 cut(s) 453
BspCNI CTCAG 1 cut(s) 48
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 1 cut(s) 303
BspPI GGATC 1 cut(s) 289
BspT104I TTCGAA 1 cut(s) 498
BspT107I GGYRCC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 21
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 170, 454
BssMI GATC 2 cut(s) 276, 281
Bst4CI ACNGT 2 cut(s) 31, 295
Bst6I CTCTTC 1 cut(s) 52
BstBAI YACGTR 1 cut(s) 322
BstBI TTCGAA 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 170, 398
BstDEI CTNAG 1 cut(s) 56
BstDSI CCRYGG 2 cut(s) 170, 454
BstF5I GGATG 1 cut(s) 50
BstFNI CGCG 1 cut(s) 172
BstHHI GCGC 2 cut(s) 62, 461
BstKTI GATC 2 cut(s) 279, 284
BstMBI GATC 2 cut(s) 276, 281
BstMWI GCNNNNNNNGC 4 cut(s) 32, 41, 257, 263
BstSLI GKGCMC 1 cut(s) 327
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 3 cut(s) 47, 199, 275
BstX2I RGATCY 1 cut(s) 281
BstYI RGATCY 1 cut(s) 281
BsuRI GGCC 1 cut(s) 453
BtgI CCRYGG 2 cut(s) 170, 454
BtsCI GGATG 1 cut(s) 50
BtsI GCAGTG 1 cut(s) 163
BtsIMutI CAGTG 3 cut(s) 36, 163, 300
Cac8I GCNNGC 2 cut(s) 170, 398
CfoI GCGC 2 cut(s) 62, 461
Cfr42I CCGCGG 1 cut(s) 173
Csp6I GTAC 2 cut(s) 316, 376
CviAII CATG 1 cut(s) 355
CviJI RGCY 7 cut(s) 38, 251, 266, 367, 385, 410, 453
CviKI_1 RGCY 7 cut(s) 38, 251, 266, 367, 385, 410, 453
CviQI GTAC 2 cut(s) 316, 376
DdeI CTNAG 1 cut(s) 56
DpnI GATC 2 cut(s) 278, 283
DpnII GATC 2 cut(s) 276, 281
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 329, 389
Eco72I CACGTG 1 cut(s) 322
FaeI CATG 1 cut(s) 358
FaiI YATR 2 cut(s) 356, 423
FaqI GGGAC 1 cut(s) 90
FatI CATG 1 cut(s) 354
FauI CCCGC 1 cut(s) 177
FblI GTMKAC 2 cut(s) 342, 441
Fnu4HI GCNGC 8 cut(s) 36, 39, 42, 213, 216, 258, 261, 264
FokI GGATG 1 cut(s) 37
Fsp4HI GCNGC 8 cut(s) 36, 39, 42, 213, 216, 258, 261, 264
FspBI CTAG 1 cut(s) 285
GlaI GCGC 2 cut(s) 61, 460
GluI GCNGC 8 cut(s) 36, 39, 42, 213, 216, 258, 261, 264
HaeIII GGCC 1 cut(s) 453
HapII CCGG 1 cut(s) 335
HhaI GCGC 2 cut(s) 62, 461
Hin1II CATG 1 cut(s) 358
Hin6I GCGC 2 cut(s) 60, 459
HinP1I GCGC 2 cut(s) 60, 459
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HpaII CCGG 1 cut(s) 335
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 3 cut(s) 316, 343, 442
Hpy188I TCNGA 3 cut(s) 244, 281, 309
Hpy188III TCNNGA 1 cut(s) 335
Hpy8I GTNNAC 3 cut(s) 316, 343, 442
Hpy99I CGWCG 3 cut(s) 440, 443, 446
HpyAV CCTTC 6 cut(s) 117, 186, 292, 304, 353, 385
HpyCH4III ACNGT 2 cut(s) 31, 295
HpyCH4IV ACGT 3 cut(s) 321, 378, 444
HpyCH4V TGCA 1 cut(s) 430
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 41, 257, 263
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 3 cut(s) 321, 378, 444
Hsp92II CATG 1 cut(s) 358
HspAI GCGC 2 cut(s) 60, 459
Kpn2I TCCGGA 1 cut(s) 334
KspI CCGCGG 1 cut(s) 173
Kzo9I GATC 2 cut(s) 276, 281
LmnI GCTCC 1 cut(s) 11
LpnPI CCDG 5 cut(s) 68, 171, 336, 340, 348
Lsp1109I GCAGC 3 cut(s) 47, 199, 275
MaeI CTAG 1 cut(s) 285
MaeII ACGT 3 cut(s) 321, 378, 444
MaeIII GTNAC 1 cut(s) 289
MalI GATC 2 cut(s) 278, 283
MboI GATC 2 cut(s) 276, 281
MboII GAAGA 1 cut(s) 39
MflI RGATCY 1 cut(s) 281
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 2 cut(s) 182, 198
MroI TCCGGA 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 89
MseI TTAA 3 cut(s) 185, 465, 508
MspA1I CMGCKG 1 cut(s) 172
MspI CCGG 1 cut(s) 335
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 4 cut(s) 32, 41, 257, 263
NdeII GATC 2 cut(s) 276, 281
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 1 cut(s) 303
NspV TTCGAA 1 cut(s) 498
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 441
PdmI GAANNNNTTC 1 cut(s) 89
PkrI GCNGC 8 cut(s) 37, 40, 43, 214, 217, 259, 262, 265
PmaCI CACGTG 1 cut(s) 322
PmlI CACGTG 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 322
PsiI TTATAA 1 cut(s) 423
PspCI CACGTG 1 cut(s) 322
PspN4I GGNNCC 1 cut(s) 303
PsuI RGATCY 1 cut(s) 281
RsaI GTAC 2 cut(s) 317, 377
RsaNI GTAC 2 cut(s) 316, 376
SacII CCGCGG 1 cut(s) 173
SalI GTCGAC 1 cut(s) 440
SaqAI TTAA 3 cut(s) 185, 465, 508
SatI GCNGC 8 cut(s) 36, 39, 42, 213, 216, 258, 261, 264
Sau3AI GATC 2 cut(s) 276, 281
SduI GDGCHC 1 cut(s) 327
Sfr303I CCGCGG 1 cut(s) 173
SfuI TTCGAA 1 cut(s) 498
SgrBI CCGCGG 1 cut(s) 173
SgrDI CGTCGACG 1 cut(s) 440
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 2 cut(s) 182, 198
SsiI CCGC 9 cut(s) 39, 42, 170, 172, 216, 255, 258, 261, 400
SspMI CTAG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 31, 295
TaiI ACGT 3 cut(s) 324, 381, 447
TaqI TCGA 2 cut(s) 441, 498
TasI AATT 2 cut(s) 182, 198
TauI GCSGC 5 cut(s) 41, 44, 218, 260, 263
Tru1I TTAA 3 cut(s) 185, 465, 508
Tru9I TTAA 3 cut(s) 185, 465, 508
TscAI CASTG 3 cut(s) 36, 170, 300
TseI GCWGC 3 cut(s) 35, 212, 263
TspDTI ATGAA 3 cut(s) 39, 98, 261
TspRI CASTG 3 cut(s) 36, 170, 300
XmiI GTMKAC 2 cut(s) 342, 441
XmnI GAANNNNTTC 1 cut(s) 89
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.