Rh4BG450100

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011069
Physical Location & Seq
Forward (+)
58789389 .. 58791902
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG450100.1

Sequence Viewer

Length: 519 bp
ATGAGTATAGGAGGAGGAACAGTAGGGTTATTAAGCTCAGCCACAGCCACAGCCACAGGCATAGGCAGAGCTGCCTCGAGTACACCAGCAGATGCGGGGCAAAGGAATTCACGATTCCCTTGTTGCTGGGCAGCGGCAGGGGCAAAGCTCACTCGCGCTCACTACCCAACAACTCTAACTCCAACTCCAAGGAGGAAAAGTAGAAGGATTGCTTGCGAAGTCGCTATCGGATCCGATTTATCTCCATCAACATCATCAGAGGTAGAAGCGGCCGCCTCCTCAAAGATCGGCGCTAGGGTCAAGGTAACCGTGCCTCTCAAGGTGTACCACGTGCCCAGAGTTCCCGAGGTCGACATCACTGGCATGGAAGGTGAACTCAAGCAGTACGTTGGCCTCTGGAAGGGCAAACGGATTTCTGCCAATCTCCCTTATAAAGTACAGTTCTTTGTCGACGTCGAAGGCCGTGGCCCTGTTAAATTCTTTGCGCATCTCAAGGAGGACGAGTTCGAATACCTTTGA

Protein Analysis

172

Amino Acids

18.38

Weight (kDa)

9.6

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeThRed_A PF02941 96 - 168 1e-27 Ferredoxin thioredoxin reductase variable alpha chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08410 AT5G08410 AT5G23440
fragaria_vesca FvH4_4g37381 FvH4_4g37381
malus_domestica MD10G1109200.v1.1 MD13G1000200.v1.1 MD16G1005600.v1.1
prunus_persica Prupe.1G352600_v2.0.a1
pyrus_communis pycom13g00020 pycom2749g00070
rosa_chinensis RchiOBHm_Chr4g0447301
rosa_laevigata RLG00000005588
rosa_multiflora Rmu_ssc0000396.1_g000007
rosa_roxburghii Rroxscaffold_5G00387690
rosa_rugosa Rorug04G0379800 Rorug04G0379800
rosa_samantha Rh4BG450100 Rh4DG448500
rosa_wichuraiana Rw4G037740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AatII GACGTC 1 cut(s) 456
Acc16I TGCGCA 1 cut(s) 486
AccI GTMKAC 2 cut(s) 351, 450
AccII CGCG 1 cut(s) 156
AciI CCGC 4 cut(s) 95, 134, 269, 273
AclWI GGATC 2 cut(s) 225, 238
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 2 cut(s) 106, 476
AcvI CACGTG 1 cut(s) 331
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 4 cut(s) 82, 326, 386, 438
AfiI CCNNNNNNNGG 1 cut(s) 400
AloI GAACNNNNNNTCC 1 cut(s) 488
AluBI AGCT 3 cut(s) 36, 71, 148
AluI AGCT 3 cut(s) 36, 71, 148
AlwI GGATC 2 cut(s) 225, 238
Ama87I CYCGRG 2 cut(s) 76, 344
AoxI GGCC 4 cut(s) 270, 391, 460, 466
ApeKI GCWGC 2 cut(s) 71, 131
ApoI RAATTY 2 cut(s) 106, 476
AspLEI GCGC 3 cut(s) 158, 293, 487
AspS9I GGNCC 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 383
AsuII TTCGAA 1 cut(s) 507
AvaI CYCGRG 2 cut(s) 76, 344
BaeGI GKGCMC 1 cut(s) 336
BamHI GGATCC 1 cut(s) 230
BbrPI CACGTG 1 cut(s) 331
BbvI GCAGC 2 cut(s) 58, 143
BccI CCATC 1 cut(s) 253
BceAI ACGGC 1 cut(s) 447
BfaI CTAG 1 cut(s) 294
BfoI RGCGCY 1 cut(s) 294
BisI GCNGC 5 cut(s) 72, 132, 135, 270, 273
BlpI GCTNAGC 1 cut(s) 37
BlsI GCNGC 5 cut(s) 73, 133, 136, 271, 274
BmeT110I CYCGRG 2 cut(s) 76, 344
BmgT120I GGNCC 1 cut(s) 467
BmiI GGNNCC 1 cut(s) 232
BmsI GCATC 2 cut(s) 82, 496
Bpu1102I GCTNAGC 1 cut(s) 37
Bpu14I TTCGAA 1 cut(s) 507
BpuEI CTTGAG 3 cut(s) 302, 362, 476
BsaAI YACGTR 1 cut(s) 331
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 3 cut(s) 188, 345, 463
Bsc4I CCNNNNNNNGG 1 cut(s) 400
Bse1I ACTGG 1 cut(s) 364
BseDI CCNNGG 3 cut(s) 188, 345, 463
BseLI CCNNNNNNNGG 1 cut(s) 400
BseMII CTCAG 1 cut(s) 51
BseNI ACTGG 1 cut(s) 364
BseRI GAGGAG 2 cut(s) 27, 268
BseSI GKGCMC 1 cut(s) 336
BseX3I CGGCCG 1 cut(s) 270
BseXI GCAGC 2 cut(s) 58, 143
BseYI CCCAGC 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 4 cut(s) 272, 393, 462, 468
BsiEI CGRYCG 1 cut(s) 273
BsiHKCI CYCGRG 2 cut(s) 76, 344
BslI CCNNNNNNNGG 1 cut(s) 400
BsnI GGCC 4 cut(s) 272, 393, 462, 468
BsoBI CYCGRG 2 cut(s) 76, 344
Bsp119I TTCGAA 1 cut(s) 507
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 2 cut(s) 230, 285
Bsp1720I GCTNAGC 1 cut(s) 37
BspACI CCGC 4 cut(s) 95, 134, 269, 273
BspANI GGCC 4 cut(s) 272, 393, 462, 468
BspCNI CTCAG 1 cut(s) 50
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 232
BspPI GGATC 2 cut(s) 225, 238
BspT104I TTCGAA 1 cut(s) 507
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 3 cut(s) 188, 345, 463
BssMI GATC 2 cut(s) 230, 285
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 188
Bst4CI ACNGT 3 cut(s) 22, 310, 441
BstACI GRCGYC 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 331
BstBI TTCGAA 1 cut(s) 507
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 1 cut(s) 37
BstDSI CCRYGG 1 cut(s) 463
BstEII GGTNACC 1 cut(s) 304
BstENI CCTNNNNNAGG 1 cut(s) 398
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 3 cut(s) 158, 293, 487
BstKTI GATC 2 cut(s) 233, 288
BstMBI GATC 2 cut(s) 230, 285
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 304
BstSLI GKGCMC 1 cut(s) 336
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 58, 143
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BstZI CGGCCG 1 cut(s) 270
BsuRI GGCC 4 cut(s) 272, 393, 462, 468
BtgI CCRYGG 1 cut(s) 463
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 214
CciNI GCGGCCGC 1 cut(s) 270
CfoI GCGC 3 cut(s) 158, 293, 487
Cfr13I GGNCC 1 cut(s) 467
Csp6I GTAC 4 cut(s) 81, 325, 385, 437
CviAII CATG 1 cut(s) 364
CviQI GTAC 4 cut(s) 81, 325, 385, 437
DdeI CTNAG 1 cut(s) 37
DpnI GATC 2 cut(s) 232, 287
DpnII GATC 2 cut(s) 230, 285
EaeI YGGCCR 1 cut(s) 270
EagI CGGCCG 1 cut(s) 270
EclXI CGGCCG 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 188
Eco52I CGGCCG 1 cut(s) 270
Eco72I CACGTG 1 cut(s) 331
Eco88I CYCGRG 2 cut(s) 76, 344
Eco91I GGTNACC 1 cut(s) 304
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoO65I GGTNACC 1 cut(s) 304
EcoRI GAATTC 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 1 cut(s) 367
FaiI YATR 4 cut(s) 8, 62, 365, 432
FalI AAGNNNNNCTT 2 cut(s) 196, 228
FatI CATG 1 cut(s) 363
FauI CCCGC 1 cut(s) 88
FblI GTMKAC 2 cut(s) 351, 450
Fnu4HI GCNGC 5 cut(s) 72, 132, 135, 270, 273
Fsp4HI GCNGC 5 cut(s) 72, 132, 135, 270, 273
FspBI CTAG 1 cut(s) 294
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 3 cut(s) 157, 292, 486
GluI GCNGC 5 cut(s) 72, 132, 135, 270, 273
GsaI CCCAGC 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 4 cut(s) 272, 393, 462, 468
HhaI GCGC 3 cut(s) 158, 293, 487
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 1 cut(s) 367
Hin6I GCGC 3 cut(s) 156, 291, 485
HinP1I GCGC 3 cut(s) 156, 291, 485
HincII GTYRAC 2 cut(s) 352, 451
HindII GTYRAC 2 cut(s) 352, 451
HinfI GANTC 1 cut(s) 114
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 5 cut(s) 83, 325, 352, 374, 451
Hpy188I TCNGA 3 cut(s) 230, 235, 259
Hpy188III TCNNGA 3 cut(s) 111, 344, 397
Hpy8I GTNNAC 5 cut(s) 83, 325, 352, 374, 451
Hpy99I CGWCG 2 cut(s) 455, 458
HpyAV CCTTC 4 cut(s) 198, 362, 394, 452
HpyCH4III ACNGT 3 cut(s) 22, 310, 441
HpyCH4IV ACGT 3 cut(s) 330, 387, 453
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 37
HpySE526I ACGT 3 cut(s) 330, 387, 453
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 1 cut(s) 367
HspAI GCGC 3 cut(s) 156, 291, 485
Kzo9I GATC 2 cut(s) 230, 285
LpnPI CCDG 8 cut(s) 42, 99, 112, 123, 345, 349, 382, 483
Lsp1109I GCAGC 2 cut(s) 58, 143
LweI GCATC 2 cut(s) 82, 496
MaeI CTAG 1 cut(s) 294
MaeII ACGT 3 cut(s) 330, 387, 453
MaeIII GTNAC 1 cut(s) 304
MalI GATC 2 cut(s) 232, 287
MboI GATC 2 cut(s) 230, 285
MflI RGATCY 1 cut(s) 230
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 2 cut(s) 106, 476
MmeI TCCRAC 1 cut(s) 206
MseI TTAA 2 cut(s) 32, 474
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 134
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 2 cut(s) 230, 285
NlaIII CATG 1 cut(s) 367
NlaIV GGNNCC 1 cut(s) 232
NotI GCGGCCGC 1 cut(s) 270
NsbI TGCGCA 1 cut(s) 486
NspV TTCGAA 1 cut(s) 507
PaeR7I CTCGAG 1 cut(s) 76
PfeI GAWTC 1 cut(s) 114
PkrI GCNGC 5 cut(s) 73, 133, 136, 271, 274
PmaCI CACGTG 1 cut(s) 331
PmlI CACGTG 1 cut(s) 331
Ppu21I YACGTR 1 cut(s) 331
PsiI TTATAA 1 cut(s) 432
PspCI CACGTG 1 cut(s) 331
PspEI GGTNACC 1 cut(s) 304
PspFI CCCAGC 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 1 cut(s) 467
PspXI VCTCGAGB 1 cut(s) 76
PsuI RGATCY 1 cut(s) 230
RsaI GTAC 4 cut(s) 82, 326, 386, 438
RsaNI GTAC 4 cut(s) 81, 325, 385, 437
RseI CAYNNNNRTG 1 cut(s) 362
SalI GTCGAC 2 cut(s) 350, 449
SaqAI TTAA 2 cut(s) 32, 474
SatI GCNGC 5 cut(s) 72, 132, 135, 270, 273
Sau3AI GATC 2 cut(s) 230, 285
Sau96I GGNCC 1 cut(s) 467
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 2 cut(s) 82, 496
Sfr274I CTCGAG 1 cut(s) 76
SfuI TTCGAA 1 cut(s) 507
SlaI CTCGAG 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 4 cut(s) 76, 317, 377, 491
SmoI CTYRAG 4 cut(s) 76, 317, 377, 491
Sse9I AATT 2 cut(s) 106, 476
SsiI CCGC 4 cut(s) 95, 134, 269, 273
SspMI CTAG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 22, 310, 441
TaiI ACGT 3 cut(s) 333, 390, 456
TaqI TCGA 5 cut(s) 77, 351, 450, 456, 507
TasI AATT 2 cut(s) 106, 476
TatI WGTACW 2 cut(s) 80, 436
TauI GCSGC 3 cut(s) 137, 272, 275
TfiI GAWTC 1 cut(s) 114
Tru1I TTAA 2 cut(s) 32, 474
Tru9I TTAA 2 cut(s) 32, 474
TscAI CASTG 1 cut(s) 364
TseI GCWGC 2 cut(s) 71, 131
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 1 cut(s) 364
XagI CCTNNNNNAGG 1 cut(s) 398
XapI RAATTY 2 cut(s) 106, 476
XhoI CTCGAG 1 cut(s) 76
XmiI GTMKAC 2 cut(s) 351, 450
XspI CTAG 1 cut(s) 294
ZraI GACGTC 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.