Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004541

Physical Location & Seq

Genomic Coordinates Forward (+)

875426 .. 879441

Gene Structure 4016 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1014000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2181 bp

ATGGATCAACCACCGCACTCGCCGCCGGCAACTTCGTCTGAGGTTTCTGTTGTCGTCGATTCTGCCACCAATGATCATCGTCTCAATCGCCCTCGCATTGGTAATGGAGATACAGACAACGACAAGGGGAAAAGTAGATGGGGGAATCTGCTAGTTGCCTACAGGACGCTGGGAGTGGTGTTTGGAGGGCTTGTTACATCCCCACTGTATGTATACCCTTCAATGCATCTCAAGTCTCCCACAGAAGCTGACTATTTGGGTATCTACAGCATCATGTTCTGGACTCTCACTCTCATTGGGGTTGTCAAGTATGCCAGCATTGCTCTCAATGCCGACGATCATGGCGAAGGTGGAACTTTTGCCCTATATTCATTACTTTGCAGGCATATGAATATTGGAATCCTTAATTCAAAGCCTGCAAGTTCAAACTCAAGTCTTTCACGCTCTATCTTGGATGACGGCATAGAAAACCAGAGTGGGCTTGCCAAGTTTTTCAAGAAAAGTGTAGTTGCCAGAAGGATGTTGCTTTTTATTGCCATGTTAGGCACATGTATGGTTATTGGCGATGGTATTCTTACACCTGCAATTTCTGTTTTATCAGCAATGGATGGAGTGAGGGCGCCTTTTCCATCTCTCAGTTCATCTCTGGTCGAAGCACTTTCGGCAGGAGTCCTTATCATTCTGTTCTTGTTACAAAAGTTTGGTACATCTCGAGTGAGCTTCCTATTTTCCCCAATTATGGGTACATGGACTTTGGTTACGCCGCTCATGGGAATTTATAGCATCATACGTCATTATCCCGGCATATTTAAGGCTATATCACCACACTTTATCTTCCATTTCTTTCAAAGAAATGGCAAGGAAGGCTGGCTGTTGCTTGGTGGCACTGTCCTTTGCATCACAGGCTCCGAAGCATTGTTTGCAGATCTTGGTCATTTCAACAGAAGCTCTATTCAGATGGCTTTCCTGTTTACAATCTATCCGTCTTTGATTCTGACATATGCGGGGCAGACAGCATACCTGATCAGGAATCCAAATGATCATGATGATGGATTCTATAAGTTTATACCAAAAAGTATCTACTGGCCTATCTTTGTCATATCCACATTGGCTGCGATAGTTGCAAGCCAGTCTCTGATATCAGCCACCTTTTCTGTAATTAAGCAATCTGTAGTGCTTGATTATTTCCCTCGGGTGAAGGTTGTGCACACATCCACTAACAAAGAAGGCGAGGTTTACTCCCCCGAAGTCAACTACATCCTCATGGTTCTCTGCGTTGCTGTCATACTTATATTCGGAGATGGAAAAGAAATTGGAAATGCATTTGGTGTTGTTGTCAGTCTAGTCATGCTCATCACTACAATATTGCTGACACTGGTAATGATCATAATATGGAGAACACCACCGGTATTGGTTGCTCTTTACTTCTGTGTATTCTTCGTGATGGAAGGTGTTTACGTGAGTGCAGTCTTCACAAAGATCGCGGAAGGTGGATGGATTCCGTTTGCCATATCCTTTATACTCGCCTTCATTATGTTTGGTTGGTTCTATGGGAGACAGAGAAAGCTAGAATATGAGTTGGCTCATAAGATAACCTTAGACAGACTTGAACAGCTATTATCTGATCCCAGTGTTCAAAGGGTTCCCGGATTGTGCTTCTTTTACACCAACATTCAAGACGGGCTCACTCCGATTCTTGGACACTACATTAAAAACATGAAATCCCTTCACCAGGTCACTATGTTCACTACTCTTCGATACCTTTTGGTTCCGAAGGTTTACCCCCATGAGAGGATTGTTATCAGTGAACTCGGCCTGAAAGGAGTTTATGGGTGCGTTATTCAGTATGGCTATGCAGATGCCCTAAACCTCGAAGGAGACGATTTTGTTAGTCAATTAACTAATAGCTTGCAGGCACATATACAGAACACCTCAGGCCGTTTCCCATCTGATCCTTCAGAGGTCCGAGAACAGATATCTAATTTGGAAGCAGCGAGGAGTGCTGGCGTACTTCACGTTCGAGGGAAAACAAGGTTTTATGTTGGAAATAACTGCTGCTGGTTCGATAAAATTATGCTACCATTTTATGAAGTTCTGCACAGCAACTGTCGGTCCTCTCTGCCTGCTCTCGGGGTGCCTCTGCCTCAACGTATCGAGGTTGGAATGCTTTACGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

727

Amino Acids

80.51

Weight (kDa)

8.36

Isoelectric Point (pI)

38.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	53 - 536	6.5e-153	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	549 - 724	2.8e-15	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_6g30570 FvH4_6g30570
malus_domestica	MD13G1014000.v1.1
prunus_persica	Prupe.1G339400_v2.0.a1 Prupe.1G339400_v2.0.a1
pyrus_communis	pycom13g01190
rosa_chinensis	RchiOBHm_Chr2g0136991 RchiOBHm_Chr4g0445501
rosa_laevigata	RLG00000005736
rosa_multiflora	Rmu_co8256761.1_g000001 Rmu_co8447799.1_g000001 Rmu_sc0001374.1_g000059 Rmu_sc0001509.1_g000011 Rmu_sc0003556.1_g000028 Rmu_sc0010466.1_g000002
rosa_roxburghii	Rroxscaffold_2G00107790 Rroxscaffold_5G00385980
rosa_rugosa	Rorug02G0336800 Rorug04G0361800
rosa_samantha	Rh2AG385900 Rh2BG392800 Rh2CG373300 Rh2DG409000 Rh4AG423200 Rh4BG435200 Rh4DG431500
rosa_wichuraiana	Rw2G031580 Rw4G036300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	589
Acc36I	ACCTGC	1 cut(s)	589
AccB1I	GGYRCC	2 cut(s)	619, 2134
AccBSI	CCGCTC	1 cut(s)	766
AccI	GTMKAC	1 cut(s)	213
AccII	CGCG	1 cut(s)	1484
AciI	CCGC	5 cut(s)	14, 23, 764, 1004, 1484
AclWI	GGATC	3 cut(s)	12, 1619, 1946
AcsI	RAATTY	1 cut(s)	774
AcuI	CTGAAG	1 cut(s)	1941
AcyI	GRCGYC	1 cut(s)	620
AfaI	GTAC	3 cut(s)	706, 745, 2010
AfiI	CCNNNNNNNGG	8 cut(s)	98, 739, 740, 770, 1697, 1732, 1791, 2129
AflIII	ACRYGT	1 cut(s)	548
AgeI	ACCGGT	1 cut(s)	1405
AgsI	TTSAA	9 cut(s)	222, 411, 426, 496, 848, 940, 1610, 1637, 1676
AjnI	CCWGG	1 cut(s)	1731
AluBI	AGCT	6 cut(s)	248, 720, 948, 1567, 1615, 1908
AluI	AGCT	6 cut(s)	248, 720, 948, 1567, 1615, 1908
Alw21I	GWGCWC	1 cut(s)	1209
Alw26I	GTCTC	5 cut(s)	86, 240, 1137, 1549, 1872
Alw44I	GTGCAC	1 cut(s)	1205
AlwI	GGATC	3 cut(s)	12, 1619, 1946
AlwNI	CAGNNNCTG	3 cut(s)	248, 1135, 2106
Ama87I	CYCGRG	3 cut(s)	711, 1191, 2129
AoxI	GGCC	3 cut(s)	1085, 1813, 1936
ApaLI	GTGCAC	1 cut(s)	1205
ApeKI	GCWGC	3 cut(s)	1112, 1991, 2055
ApoI	RAATTY	1 cut(s)	774
AsiGI	ACCGGT	1 cut(s)	1405
AspLEI	GCGC	1 cut(s)	622
AspS9I	GGNCC	2 cut(s)	1963, 2112
AsuC2I	CCSGG	2 cut(s)	801, 1647
AsuHPI	GGTGA	3 cut(s)	813, 1207, 1721
AvaI	CYCGRG	3 cut(s)	711, 1191, 2129
AvaII	GGWCC	2 cut(s)	1963, 2112
AxyI	CCTNAGG	1 cut(s)	1933
BaeGI	GKGCMC	1 cut(s)	1209
BanI	GGYRCC	2 cut(s)	619, 2134
BanII	GRGCYC	1 cut(s)	1686
BbsI	GAAGAC	1 cut(s)	1462
Bbv12I	GWGCWC	1 cut(s)	1209
BbvI	GCAGC	3 cut(s)	1099, 2003, 2042
BceAI	ACGGC	2 cut(s)	475, 1923
BciT130I	CCWGG	1 cut(s)	1733
BclI	TGATCA	4 cut(s)	73, 1023, 1039, 1383
BcnI	CCSGG	2 cut(s)	801, 1647
BcoDI	GTCTC	5 cut(s)	86, 240, 1137, 1549, 1872
BfaI	CTAG	3 cut(s)	152, 1343, 1568
BfmI	CTRYAG	3 cut(s)	160, 265, 1170
BfoI	RGCGCY	1 cut(s)	623
BfuAI	ACCTGC	1 cut(s)	589
BglII	AGATCT	1 cut(s)	925
BisI	GCNGC	5 cut(s)	23, 764, 1113, 1992, 2056
BlsI	GCNGC	5 cut(s)	24, 765, 1114, 1993, 2057
Bme1390I	CCNGG	3 cut(s)	801, 1647, 1733
Bme18I	GGWCC	2 cut(s)	1963, 2112
BmeT110I	CYCGRG	3 cut(s)	711, 1191, 2129
BmgT120I	GGNCC	2 cut(s)	1963, 2112
BmiI	GGNNCC	5 cut(s)	621, 907, 1644, 1770, 2136
BmrFI	CCNGG	3 cut(s)	801, 1647, 1733
BmrI	ACTGGG	1 cut(s)	1623
BmsI	GCATC	5 cut(s)	235, 279, 792, 906, 1849
BmuI	ACTGGG	1 cut(s)	1623
BpiI	GAAGAC	1 cut(s)	1462
BplI	GAGNNNNNCTC	2 cut(s)	1223, 1255
BpuEI	CTTGAG	2 cut(s)	215, 415
BpuMI	CCSGG	2 cut(s)	801, 1647
BsaAI	YACGTR	1 cut(s)	1459
BsaBI	GATNNNNATC	1 cut(s)	1799
BsaHI	GRCGYC	1 cut(s)	620
BsaJI	CCNNGG	1 cut(s)	1190
BsaWI	WCCGGW	1 cut(s)	1405
BsaXI	ACNNNNNCTCC	2 cut(s)	177, 207
Bsc4I	CCNNNNNNNGG	8 cut(s)	98, 739, 740, 770, 1697, 1732, 1791, 2129
Bse118I	RCCGGY	2 cut(s)	25, 1405
Bse1I	ACTGG	4 cut(s)	1088, 1129, 1380, 1629
Bse21I	CCTNAGG	1 cut(s)	1933
Bse3DI	GCAATG	2 cut(s)	318, 609
Bse8I	GATNNNNATC	1 cut(s)	1799
BseBI	CCWGG	1 cut(s)	1733
BseDI	CCNNGG	1 cut(s)	1190
BseGI	GGATG	7 cut(s)	197, 460, 525, 613, 1211, 1257, 1499
BseJI	GATNNNNATC	1 cut(s)	1799
BseLI	CCNNNNNNNGG	8 cut(s)	98, 739, 740, 770, 1697, 1732, 1791, 2129
BseMI	GCAATG	2 cut(s)	318, 609
BseMII	CTCAG	3 cut(s)	30, 649, 1947
BseNI	ACTGG	4 cut(s)	1088, 1129, 1380, 1629
BseRI	GAGGAG	1 cut(s)	2011
BseSI	GKGCMC	1 cut(s)	1209
BseXI	GCAGC	3 cut(s)	1099, 2003, 2042
BseYI	CCCAGC	1 cut(s)	169
BsgI	GTGCAG	2 cut(s)	1485, 2081
Bsh1236I	CGCG	1 cut(s)	1484
BshFI	GGCC	3 cut(s)	1087, 1815, 1938
BshNI	GGYRCC	2 cut(s)	619, 2134
BshTI	ACCGGT	1 cut(s)	1405
BsiHKAI	GWGCWC	1 cut(s)	1209
BsiHKCI	CYCGRG	3 cut(s)	711, 1191, 2129
BsiSI	CCGG	4 cut(s)	26, 801, 1406, 1647
BslI	CCNNNNNNNGG	8 cut(s)	98, 739, 740, 770, 1697, 1732, 1791, 2129
BsmAI	GTCTC	5 cut(s)	86, 240, 1137, 1549, 1872
BsmBI	CGTCTC	2 cut(s)	86, 1872
BsmI	GAATGC	1 cut(s)	2169
BsnI	GGCC	3 cut(s)	1087, 1815, 1938
BsoBI	CYCGRG	3 cut(s)	711, 1191, 2129
Bsp1286I	GDGCHC	2 cut(s)	1209, 1686
BspACI	CCGC	5 cut(s)	14, 23, 764, 1004, 1484
BspANI	GGCC	3 cut(s)	1087, 1815, 1938
BspCNI	CTCAG	3 cut(s)	31, 648, 1946
BspFNI	CGCG	1 cut(s)	1484
BspHI	TCATGA	1 cut(s)	1042
BspLI	GGNNCC	5 cut(s)	621, 907, 1644, 1770, 2136
BspMI	ACCTGC	1 cut(s)	589
BspPI	GGATC	3 cut(s)	12, 1619, 1946
BspT107I	GGYRCC	2 cut(s)	619, 2134
BsrBI	CCGCTC	1 cut(s)	766
BsrDI	GCAATG	2 cut(s)	318, 609
BsrFI	RCCGGY	2 cut(s)	25, 1405
BsrI	ACTGG	4 cut(s)	1088, 1129, 1380, 1629
BssAI	RCCGGY	2 cut(s)	25, 1405
BssECI	CCNNGG	1 cut(s)	1190
BssNAI	GTATAC	1 cut(s)	214
BssNI	GRCGYC	1 cut(s)	620
Bst1107I	GTATAC	1 cut(s)	214
Bst2UI	CCWGG	1 cut(s)	1733
Bst4CI	ACNGT	3 cut(s)	207, 889, 2108
Bst6I	CTCTTC	1 cut(s)	1758
BstACI	GRCGYC	1 cut(s)	620
BstAPI	GCANNNNNTGC	1 cut(s)	920
BstBAI	YACGTR	1 cut(s)	1459
BstDEI	CTNAG	4 cut(s)	39, 635, 1597, 1933
BstENI	CCTNNNNNAGG	1 cut(s)	1730
BstF5I	GGATG	7 cut(s)	197, 460, 525, 613, 1211, 1257, 1499
BstFNI	CGCG	1 cut(s)	1484
BstH2I	RGCGCY	1 cut(s)	623
BstHHI	GCGC	1 cut(s)	622
BstMAI	GTCTC	5 cut(s)	86, 240, 1137, 1549, 1872
BstMWI	GCNNNNNNNGC	9 cut(s)	22, 320, 329, 662, 864, 903, 920, 1121, 2000
BstNI	CCWGG	1 cut(s)	1733
BstNSI	RCATGY	1 cut(s)	552
BstSCI	CCNGG	3 cut(s)	799, 1645, 1731
BstSFI	CTRYAG	3 cut(s)	160, 265, 1170
BstSLI	GKGCMC	1 cut(s)	1209
BstUI	CGCG	1 cut(s)	1484
BstV1I	GCAGC	3 cut(s)	1099, 2003, 2042
BstV2I	GAAGAC	1 cut(s)	1462
BstX2I	RGATCY	1 cut(s)	925
BstYI	RGATCY	1 cut(s)	925
BstZ17I	GTATAC	1 cut(s)	214
Bsu36I	CCTNAGG	1 cut(s)	1933
BsuRI	GGCC	3 cut(s)	1087, 1815, 1938
BtgZI	GCGATG	1 cut(s)	579
BtsCI	GGATG	7 cut(s)	197, 460, 525, 613, 1211, 1257, 1499
BtsIMutI	CAGTG	5 cut(s)	203, 885, 1373, 1636, 1810
BveI	ACCTGC	1 cut(s)	589
CaiI	CAGNNNCTG	3 cut(s)	248, 1135, 2106
CciI	TCATGA	1 cut(s)	1042
CfoI	GCGC	1 cut(s)	622
Cfr10I	RCCGGY	2 cut(s)	25, 1405
Cfr13I	GGNCC	2 cut(s)	1963, 2112
CseI	GACGC	1 cut(s)	175
CsiI	ACCWGGT	1 cut(s)	1731
Csp6I	GTAC	3 cut(s)	705, 744, 2009
CspAI	ACCGGT	1 cut(s)	1405
CspCI	CAANNNNNGTGG	2 cut(s)	1392, 1427
CviQI	GTAC	3 cut(s)	705, 744, 2009
DdeI	CTNAG	4 cut(s)	39, 635, 1597, 1933
DinI	GGCGCC	1 cut(s)	621
Eam1104I	CTCTTC	1 cut(s)	1758
EarI	CTCTTC	1 cut(s)	1758
Eco24I	GRGCYC	1 cut(s)	1686
Eco32I	GATATC	2 cut(s)	1140, 1977
Eco47I	GGWCC	2 cut(s)	1963, 2112
Eco57I	CTGAAG	1 cut(s)	1941
Eco81I	CCTNAGG	1 cut(s)	1933
Eco88I	CYCGRG	3 cut(s)	711, 1191, 2129
EcoNI	CCTNNNNNAGG	1 cut(s)	1730
EcoRII	CCWGG	1 cut(s)	1731
EcoRV	GATATC	2 cut(s)	1140, 1977
EcoT22I	ATGCAT	2 cut(s)	228, 1324
EcoT38I	GRGCYC	1 cut(s)	1686
EgeI	GGCGCC	1 cut(s)	621
EheI	GGCGCC	1 cut(s)	621
Esp3I	CGTCTC	2 cut(s)	86, 1872
FalI	AAGNNNNNCTT	2 cut(s)	1580, 1612
FauI	CCCGC	1 cut(s)	997
FauNDI	CATATG	2 cut(s)	387, 1000
FbaI	TGATCA	4 cut(s)	73, 1023, 1039, 1383
FblI	GTMKAC	1 cut(s)	213
Fnu4HI	GCNGC	5 cut(s)	23, 764, 1113, 1992, 2056
FokI	GGATG	7 cut(s)	184, 467, 532, 620, 1198, 1244, 1506
FriOI	GRGCYC	1 cut(s)	1686
Fsp4HI	GCNGC	5 cut(s)	23, 764, 1113, 1992, 2056
FspBI	CTAG	3 cut(s)	152, 1343, 1568
GlaI	GCGC	1 cut(s)	621
GluI	GCNGC	5 cut(s)	23, 764, 1113, 1992, 2056
GsaI	CCCAGC	1 cut(s)	173
HaeII	RGCGCY	1 cut(s)	623
HaeIII	GGCC	3 cut(s)	1087, 1815, 1938
HapII	CCGG	4 cut(s)	26, 801, 1406, 1647
HgaI	GACGC	1 cut(s)	175
HhaI	GCGC	1 cut(s)	622
Hin1I	GRCGYC	1 cut(s)	620
Hin6I	GCGC	1 cut(s)	620
HinP1I	GCGC	1 cut(s)	620
HincII	GTYRAC	1 cut(s)	1252
HindII	GTYRAC	1 cut(s)	1252
HpaII	CCGG	4 cut(s)	26, 801, 1406, 1647
HphI	GGTGA	3 cut(s)	813, 1207, 1721
Hpy166II	GTNNAC	9 cut(s)	214, 972, 1207, 1237, 1252, 1456, 1746, 1780, 1808
Hpy188III	TCNNGA	7 cut(s)	280, 496, 711, 1027, 1043, 1441, 1676
Hpy8I	GTNNAC	9 cut(s)	214, 972, 1207, 1237, 1252, 1456, 1746, 1780, 1808
Hpy99I	CGWCG	2 cut(s)	59, 338
HpyCH4III	ACNGT	3 cut(s)	207, 889, 2108
HpyCH4IV	ACGT	4 cut(s)	790, 1458, 2016, 2149
HpyF10VI	GCNNNNNNNGC	9 cut(s)	22, 320, 329, 662, 864, 903, 920, 1121, 2000
HpyF3I	CTNAG	4 cut(s)	39, 635, 1597, 1933
HpySE526I	ACGT	4 cut(s)	790, 1458, 2016, 2149
Hsp92I	GRCGYC	1 cut(s)	620
HspAI	GCGC	1 cut(s)	620
KasI	GGCGCC	1 cut(s)	619
KroI	GCCGGC	1 cut(s)	25
KroNI	GCCGGC	1 cut(s)	27
Ksp22I	TGATCA	4 cut(s)	73, 1023, 1039, 1383
LmnI	GCTCC	1 cut(s)	911
Lsp1109I	GCAGC	3 cut(s)	1099, 2003, 2042
LweI	GCATC	5 cut(s)	235, 279, 792, 906, 1849
MabI	ACCWGGT	1 cut(s)	1731
MaeI	CTAG	3 cut(s)	152, 1343, 1568
MaeII	ACGT	4 cut(s)	790, 1458, 2016, 2149
MaeIII	GTNAC	4 cut(s)	193, 690, 757, 1735
MbiI	CCGCTC	1 cut(s)	766
MboII	GAAGA	4 cut(s)	826, 1429, 1462, 1745
MflI	RGATCY	1 cut(s)	925
MhlI	GDGCHC	2 cut(s)	1209, 1686
MluCI	AATT	9 cut(s)	406, 585, 735, 774, 1158, 1311, 1895, 1981, 2070
Mly113I	GGCGCC	1 cut(s)	620
MlyI	GAGTC	2 cut(s)	277, 678
MmeI	TCCRAC	2 cut(s)	2023, 2140
Mph1103I	ATGCAT	2 cut(s)	228, 1324
MroNI	GCCGGC	1 cut(s)	25
MseI	TTAA	5 cut(s)	405, 810, 1161, 1710, 1898
MslI	CAYNNNNRTG	3 cut(s)	551, 1047, 1262
MspI	CCGG	4 cut(s)	26, 801, 1406, 1647
MspR9I	CCNGG	3 cut(s)	801, 1647, 1733
Mva1269I	GAATGC	1 cut(s)	2169
MvaI	CCWGG	1 cut(s)	1733
MvnI	CGCG	1 cut(s)	1484
MwoI	GCNNNNNNNGC	9 cut(s)	22, 320, 329, 662, 864, 903, 920, 1121, 2000
NaeI	GCCGGC	1 cut(s)	27
NarI	GGCGCC	1 cut(s)	620
NciI	CCSGG	2 cut(s)	801, 1647
NdeI	CATATG	2 cut(s)	387, 1000
NgoMIV	GCCGGC	1 cut(s)	25
NlaIV	GGNNCC	5 cut(s)	621, 907, 1644, 1770, 2136
NmeAIII	GCCGAG	1 cut(s)	1791
NmuCI	GTSAC	1 cut(s)	1735
NsiI	ATGCAT	2 cut(s)	228, 1324
NspI	RCATGY	1 cut(s)	552
PaeR7I	CTCGAG	1 cut(s)	711
PagI	TCATGA	1 cut(s)	1042
PaqCI	CACCTGC	1 cut(s)	589
PciI	ACATGT	1 cut(s)	548
PcsI	WCGNNNNNNNCGW	2 cut(s)	342, 1878
PctI	GAATGC	1 cut(s)	2169
PdiI	GCCGGC	1 cut(s)	27
PfeI	GAWTC	8 cut(s)	59, 145, 399, 991, 1030, 1053, 1498, 1693
PfoI	TCCNGGA	1 cut(s)	1645
PinAI	ACCGGT	1 cut(s)	1405
PkrI	GCNGC	5 cut(s)	24, 765, 1114, 1993, 2057
PleI	GAGTC	2 cut(s)	277, 677
PluTI	GGCGCC	1 cut(s)	623
PpsI	GAGTC	2 cut(s)	277, 677
Ppu21I	YACGTR	1 cut(s)	1459
PscI	ACATGT	1 cut(s)	548
Psp6I	CCWGG	1 cut(s)	1731
PspFI	CCCAGC	1 cut(s)	169
PspGI	CCWGG	1 cut(s)	1731
PspN4I	GGNNCC	5 cut(s)	621, 907, 1644, 1770, 2136
PspPI	GGNCC	2 cut(s)	1963, 2112
PsrI	GAACNNNNNNTAC	2 cut(s)	2001, 2033
PstNI	CAGNNNCTG	3 cut(s)	248, 1135, 2106
PsuI	RGATCY	1 cut(s)	925
RsaI	GTAC	3 cut(s)	706, 745, 2010
RsaNI	GTAC	3 cut(s)	705, 744, 2009
RseI	CAYNNNNRTG	3 cut(s)	551, 1047, 1262
SaqAI	TTAA	5 cut(s)	405, 810, 1161, 1710, 1898
SatI	GCNGC	5 cut(s)	23, 764, 1113, 1992, 2056
Sau96I	GGNCC	2 cut(s)	1963, 2112
SchI	GAGTC	2 cut(s)	277, 678
ScrFI	CCNGG	3 cut(s)	801, 1647, 1733
SduI	GDGCHC	2 cut(s)	1209, 1686
SexAI	ACCWGGT	1 cut(s)	1731
SfaNI	GCATC	5 cut(s)	235, 279, 792, 906, 1849
SfcI	CTRYAG	3 cut(s)	160, 265, 1170
SfoI	GGCGCC	1 cut(s)	621
Sfr274I	CTCGAG	1 cut(s)	711
SinI	GGWCC	2 cut(s)	1963, 2112
SlaI	CTCGAG	1 cut(s)	711
SmiMI	CAYNNNNRTG	3 cut(s)	551, 1047, 1262
SmlI	CTYRAG	3 cut(s)	230, 430, 711
SmoI	CTYRAG	3 cut(s)	230, 430, 711
Sse9I	AATT	9 cut(s)	406, 585, 735, 774, 1158, 1311, 1895, 1981, 2070
SsiI	CCGC	5 cut(s)	14, 23, 764, 1004, 1484
SspDI	GGCGCC	1 cut(s)	619
SspI	AATATT	2 cut(s)	394, 1365
SspMI	CTAG	3 cut(s)	152, 1343, 1568
StyD4I	CCNGG	3 cut(s)	799, 1645, 1731
TaaI	ACNGT	3 cut(s)	207, 889, 2108
TaiI	ACGT	4 cut(s)	793, 1461, 2019, 2152
TaqI	TCGA	8 cut(s)	57, 651, 712, 1756, 1872, 2020, 2064, 2154
TaqII	GACCGA	1 cut(s)	2100
TasI	AATT	9 cut(s)	406, 585, 735, 774, 1158, 1311, 1895, 1981, 2070
TauI	GCSGC	2 cut(s)	25, 766
TfiI	GAWTC	8 cut(s)	59, 145, 399, 991, 1030, 1053, 1498, 1693
Tru1I	TTAA	5 cut(s)	405, 810, 1161, 1710, 1898
Tru9I	TTAA	5 cut(s)	405, 810, 1161, 1710, 1898
TscAI	CASTG	5 cut(s)	210, 892, 1380, 1636, 1810
TseFI	GTSAC	1 cut(s)	1735
TseI	GCWGC	3 cut(s)	1112, 1991, 2055
Tsp45I	GTSAC	1 cut(s)	1735
TspDTI	ATGAA	6 cut(s)	360, 404, 630, 1519, 1733, 2103
TspGWI	ACGGA	2 cut(s)	972, 1491
TspRI	CASTG	5 cut(s)	210, 892, 1380, 1636, 1810
VneI	GTGCAC	1 cut(s)	1205
VpaK11BI	GGWCC	2 cut(s)	1963, 2112
XagI	CCTNNNNNAGG	1 cut(s)	1730
XapI	RAATTY	1 cut(s)	774
XceI	RCATGY	1 cut(s)	552
XhoI	CTCGAG	1 cut(s)	711
XmiI	GTMKAC	1 cut(s)	213
XspI	CTAG	3 cut(s)	152, 1343, 1568
Zsp2I	ATGCAT	2 cut(s)	228, 1324

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.