Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004541

Physical Location & Seq

Genomic Coordinates Reverse (-)

65939697 .. 65943635

Gene Structure 3939 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ41309

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2067 bp

ATGCCAATCCATTCCAGTCAGGGAAAAGGTGGAAGGGAGAATTTGCTACTTGCATACAAGACACTGGGAGTAGTGTTTGGAGGACTTGTTACATCCCCACTGTATGTGTACCCTTCCATGCCACTCAAGTCTCCGACCGAAGATGACTATTTGGGCATTTACAGCATTATGTTTTGGACTCTTACCCTCATTGGGGTTGTCAAATATGCTGGCATTGCTCTCAAAGCCGATGATCAAGGCGAAGGTGGGACTTTTGCCTTGTATTCATTACTTTGCAGGAATATGAATATTGGAATCCTCTCTTCAAATCCAAACTCAAGTCTTTCACACTCTATCTATGATGAGGGCATTGAAAATCAGAGTAGGCTTGCAAAAATTTTCAAGAAAAGTATAGTTGCTAGAAGGTTATTGCTTTTTATTGCCATGTTAGGCATGTGCATGGTTATTGGAGATGGCATTCTTACACCTGCAATATCTGTTTTGTCAGCAATGGATGGAGTAAGGGCACCTTTTCCATCTATCAAATCATCTGTTGTGGAAGCGCTTTCTGCAGTAGTCCTTATCCTTCTTTTTTTATTACAAAAGTTTGGTACATCTCGAGTGAGCTTCCTATTTTCCCCCATTATGGGTGCATGGACTTTGAGTACCCCGCTCGTAGGAATTTATAGCATCATAAGTCATTATCCAAGCATATTCAAGGCCATATCACCACACTATATCTTCCGTTTCTTTTGGAGAAACGGTAAGGAAGGCTGGTTGTTGCTTAGTGGAACTGTCCTTTGCATCACAGGGTCCGAGGCATTGTTTGCAGATCTTGGTCATTTCAACCGAAGCTCCATTCAGATAGCTTTCTTGTTTACCATCTATCCTTCTCTGGTTCTGACATATGCGGGGCAGACAGCATACCTGATCAAGAATCCAAATGATCATAATGATGGATTCTATAAGTTTATACCAACTACTATCTACTGGCCTATCTTTATCATATCTACATTAGCTGCAATAGTTGCAAGCCAGTCTCTGATATCAGCCACGTTTTCGGTAATCAAGCAATCTGTAGTACTTGATTATTTCCCTCGAGTGAAGGTTGTGCACACATCCCCTAGCAAAGAAGGCGAGGTTTACTCCCCTGAAGTCAATTACATTCTCATGGTTCTCTGTGTTGCTGTCATACTTATATTTGGGGATGGAAAAGACATAGGAAATGCATTTGGTGTTGTTGTCAGCTTAGTCATGCTCATCACCACAGTATTACTAACACTGGTAATGGTCATAATATGGAGAACACCACCGGTGCTGGTGGCTCTCTACTTCTCTATCTTCTTTGTGATGGAAGGCGTTTATGTGAGTGCAGTGTTCACCAAGATCACTGAAGGTGGATGGATTCCTTTTGCCATATCTTTTATCCTTGCCTTCATTATGTTCGGTTGGTACTATGGAAGACAAAGGAAGATAGAGTACGAGTTGACTCACAAAATAACCCTAGACAAACTTGGACTGCTATTATCTGATCCCAGTGTTCAAAGGGTTCCTGGATTCTGCTTGTTTTACTCCAACATTCAAGATGGGCTTACTCCGATTCTCGGACACTATATTAAAAACATGAAATCCCTTCACCAGGTCACCGTGTTCACTACTCTTCGATACTTATTGGTTCCTAAGGTTGCTCCTCATGAGAGGATTGTTATCTGCAAACTTGGCCTGAAAGGGGTTTATGGGTGTGTTATTCAGTATGGCTACGCAGATCCCCTAAACCTCGAAGGAGAAGATTTTGTCAGCCAAGTTACAAATAGTTTGCTGGCACATATACAGGACAGCTCGGGCTGTCGGCCATCTGATCCTGCAGAGATCCAACAAGAGATGTCTAATTTGGAAGAAGCAATGAGTGCTGGAATAGTTCATATTCGAGGAAAGACAAGGTTTTATATTGGCAAGAGTTGTGGCTGGTTTGATAAAATTATGCTCGCATTTTATGAAGTTCTGCACAGTAACTGTAGGTCTGCTCTGCCTGCTCTTGGGGTGCCACTACCTCAGCGTATCGAGGTTGGAATGCTCTATGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

688

Amino Acids

76.17

Weight (kDa)

8.58

Isoelectric Point (pI)

41.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	18 - 497	9.9e-153	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	511 - 686	1.8e-16	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_6g30570 FvH4_6g30570
malus_domestica	MD13G1014000.v1.1
prunus_persica	Prupe.1G339400_v2.0.a1 Prupe.1G339400_v2.0.a1
pyrus_communis	pycom13g01190
rosa_chinensis	RchiOBHm_Chr2g0136991 RchiOBHm_Chr4g0445501
rosa_laevigata	RLG00000005736
rosa_multiflora	Rmu_co8256761.1_g000001 Rmu_co8447799.1_g000001 Rmu_sc0001374.1_g000059 Rmu_sc0001509.1_g000011 Rmu_sc0003556.1_g000028 Rmu_sc0010466.1_g000002
rosa_roxburghii	Rroxscaffold_2G00107790 Rroxscaffold_5G00385980
rosa_rugosa	Rorug02G0336800 Rorug04G0361800
rosa_samantha	Rh2AG385900 Rh2BG392800 Rh2CG373300 Rh2DG409000 Rh4AG423200 Rh4BG435200 Rh4DG431500
rosa_wichuraiana	Rw2G031580 Rw4G036300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	475
Acc36I	ACCTGC	1 cut(s)	475
AccB1I	GGYRCC	2 cut(s)	505, 2020
AccBSI	CCGCTC	1 cut(s)	652
AciI	CCGC	2 cut(s)	650, 890
AclWI	GGATC	4 cut(s)	1505, 1739, 1832, 1843
AcoI	YGGCCR	1 cut(s)	1829
AcsI	RAATTY	3 cut(s)	40, 375, 660
AcuI	CTGAAG	2 cut(s)	1152, 1392
AfaI	GTAC	6 cut(s)	110, 592, 646, 1062, 1433, 1460
AfeI	AGCGCT	1 cut(s)	543
AfiI	CCNNNNNNNGG	9 cut(s)	192, 193, 625, 626, 656, 1583, 1618, 1708, 2015
AgeI	ACCGGT	1 cut(s)	1291
AgsI	TTSAA	7 cut(s)	306, 353, 382, 697, 826, 1523, 1562
AjnI	CCWGG	2 cut(s)	1531, 1617
AluBI	AGCT	6 cut(s)	606, 834, 848, 998, 1227, 1818
AluI	AGCT	6 cut(s)	606, 834, 848, 998, 1227, 1818
Alw21I	GWGCWC	1 cut(s)	1095
Alw26I	GTCTC	2 cut(s)	135, 1023
Alw44I	GTGCAC	1 cut(s)	1091
AlwI	GGATC	4 cut(s)	1505, 1739, 1832, 1843
AlwNI	CAGNNNCTG	2 cut(s)	1021, 1992
Ama87I	CYCGRG	3 cut(s)	597, 1077, 1819
Aor51HI	AGCGCT	1 cut(s)	543
AoxI	GGCC	4 cut(s)	699, 971, 1699, 1829
ApaLI	GTGCAC	1 cut(s)	1091
ApeKI	GCWGC	1 cut(s)	998
ApoI	RAATTY	3 cut(s)	40, 375, 660
AsiGI	ACCGGT	1 cut(s)	1291
AspLEI	GCGC	1 cut(s)	544
AspS9I	GGNCC	1 cut(s)	792
AsuHPI	GGTGA	5 cut(s)	699, 1234, 1351, 1607, 1615
AvaI	CYCGRG	3 cut(s)	597, 1077, 1819
AvaII	GGWCC	1 cut(s)	792
AxyI	CCTNAGG	1 cut(s)	1659
BaeGI	GKGCMC	2 cut(s)	508, 1095
BanI	GGYRCC	2 cut(s)	505, 2020
BarI	GAAGNNNNNNTAC	2 cut(s)	1442, 1474
BbsI	GAAGAC	1 cut(s)	1447
Bbv12I	GWGCWC	1 cut(s)	1095
BbvCI	CCTCAGC	1 cut(s)	2031
BbvI	GCAGC	1 cut(s)	985
BciT130I	CCWGG	2 cut(s)	1533, 1619
BclI	TGATCA	3 cut(s)	232, 909, 925
BcoDI	GTCTC	2 cut(s)	135, 1023
BfaI	CTAG	3 cut(s)	399, 1104, 1484
BfmI	CTRYAG	4 cut(s)	549, 1056, 1842, 1993
BfoI	RGCGCY	1 cut(s)	545
BfuAI	ACCTGC	1 cut(s)	475
BglII	AGATCT	1 cut(s)	811
BisI	GCNGC	1 cut(s)	999
BlsI	GCNGC	1 cut(s)	1000
BmcAI	AGTACT	1 cut(s)	1062
Bme1390I	CCNGG	2 cut(s)	1533, 1619
Bme18I	GGWCC	1 cut(s)	792
BmeT110I	CYCGRG	3 cut(s)	597, 1077, 1819
BmgT120I	GGNCC	1 cut(s)	792
BmiI	GGNNCC	5 cut(s)	507, 793, 1530, 1656, 2022
BmrFI	CCNGG	2 cut(s)	1533, 1619
BmrI	ACTGGG	2 cut(s)	74, 1509
BmsI	GCATC	2 cut(s)	678, 792
BmuI	ACTGGG	2 cut(s)	74, 1509
BpiI	GAAGAC	1 cut(s)	1447
BplI	GAGNNNNNCTC	2 cut(s)	1109, 1141
Bpu10I	CCTNAGC	1 cut(s)	2031
BpuEI	CTTGAG	2 cut(s)	110, 301
BsaBI	GATNNNNATC	1 cut(s)	1685
BsaJI	CCNNGG	1 cut(s)	795
BsaWI	WCCGGW	1 cut(s)	1291
BsaXI	ACNNNNNCTCC	6 cut(s)	72, 102, 727, 757, 818, 848
Bsc4I	CCNNNNNNNGG	9 cut(s)	192, 193, 625, 626, 656, 1583, 1618, 1708, 2015
Bse118I	RCCGGY	1 cut(s)	1291
Bse1I	ACTGG	6 cut(s)	15, 69, 974, 1015, 1266, 1515
Bse21I	CCTNAGG	1 cut(s)	1659
Bse3DI	GCAATG	3 cut(s)	213, 495, 1887
Bse8I	GATNNNNATC	1 cut(s)	1685
BseBI	CCWGG	2 cut(s)	1533, 1619
BseDI	CCNNGG	1 cut(s)	795
BseGI	GGATG	5 cut(s)	92, 499, 1097, 1192, 1385
BseJI	GATNNNNATC	1 cut(s)	1685
BseLI	CCNNNNNNNGG	9 cut(s)	192, 193, 625, 626, 656, 1583, 1618, 1708, 2015
BseMI	GCAATG	3 cut(s)	213, 495, 1887
BseMII	CTCAG	1 cut(s)	2045
BseNI	ACTGG	6 cut(s)	15, 69, 974, 1015, 1266, 1515
BseRI	GAGGAG	1 cut(s)	1659
BseSI	GKGCMC	2 cut(s)	508, 1095
BseXI	GCAGC	1 cut(s)	985
BsgI	GTGCAG	2 cut(s)	1371, 1967
Bsh1285I	CGRYCG	1 cut(s)	138
BshFI	GGCC	4 cut(s)	701, 973, 1701, 1831
BshNI	GGYRCC	2 cut(s)	505, 2020
BshTI	ACCGGT	1 cut(s)	1291
BsiEI	CGRYCG	1 cut(s)	138
BsiHKAI	GWGCWC	1 cut(s)	1095
BsiHKCI	CYCGRG	3 cut(s)	597, 1077, 1819
BsiSI	CCGG	1 cut(s)	1292
BslFI	GGGAC	1 cut(s)	262
BslI	CCNNNNNNNGG	9 cut(s)	192, 193, 625, 626, 656, 1583, 1618, 1708, 2015
BsmAI	GTCTC	2 cut(s)	135, 1023
BsmFI	GGGAC	1 cut(s)	262
BsmI	GAATGC	2 cut(s)	456, 2055
BsnI	GGCC	4 cut(s)	701, 973, 1701, 1831
BsoBI	CYCGRG	3 cut(s)	597, 1077, 1819
Bsp1286I	GDGCHC	2 cut(s)	508, 1095
Bsp143I	GATC	9 cut(s)	232, 811, 909, 925, 1365, 1510, 1744, 1837, 1848
BspACI	CCGC	2 cut(s)	650, 890
BspANI	GGCC	4 cut(s)	701, 973, 1701, 1831
BspCNI	CTCAG	1 cut(s)	2044
BspHI	TCATGA	1 cut(s)	1672
BspLI	GGNNCC	5 cut(s)	507, 793, 1530, 1656, 2022
BspMAI	CTGCAG	2 cut(s)	553, 1846
BspMI	ACCTGC	1 cut(s)	475
BspPI	GGATC	4 cut(s)	1505, 1739, 1832, 1843
BspT107I	GGYRCC	2 cut(s)	505, 2020
BsrBI	CCGCTC	1 cut(s)	652
BsrDI	GCAATG	3 cut(s)	213, 495, 1887
BsrFI	RCCGGY	1 cut(s)	1291
BsrI	ACTGG	6 cut(s)	15, 69, 974, 1015, 1266, 1515
BssAI	RCCGGY	1 cut(s)	1291
BssECI	CCNNGG	1 cut(s)	795
BssMI	GATC	9 cut(s)	232, 811, 909, 925, 1365, 1510, 1744, 1837, 1848
Bst2UI	CCWGG	2 cut(s)	1533, 1619
Bst4CI	ACNGT	7 cut(s)	102, 743, 775, 1249, 1627, 1988, 1994
Bst6I	CTCTTC	2 cut(s)	307, 1644
BstAPI	GCANNNNNTGC	3 cut(s)	806, 1007, 1886
BstC8I	GCNNGC	6 cut(s)	211, 369, 1012, 1800, 1965, 2010
BstDEI	CTNAG	4 cut(s)	764, 1228, 1659, 2031
BstEII	GGTNACC	1 cut(s)	1621
BstENI	CCTNNNNNAGG	1 cut(s)	1616
BstF5I	GGATG	5 cut(s)	92, 499, 1097, 1192, 1385
BstH2I	RGCGCY	1 cut(s)	545
BstHHI	GCGC	1 cut(s)	544
BstKTI	GATC	9 cut(s)	235, 814, 912, 928, 1368, 1513, 1747, 1840, 1851
BstMAI	GTCTC	2 cut(s)	135, 1023
BstMBI	GATC	9 cut(s)	232, 811, 909, 925, 1365, 1510, 1744, 1837, 1848
BstMCI	CGRYCG	1 cut(s)	138
BstNI	CCWGG	2 cut(s)	1533, 1619
BstNSI	RCATGY	1 cut(s)	436
BstPI	GGTNACC	1 cut(s)	1621
BstSCI	CCNGG	2 cut(s)	1531, 1617
BstSFI	CTRYAG	4 cut(s)	549, 1056, 1842, 1993
BstSLI	GKGCMC	2 cut(s)	508, 1095
BstV1I	GCAGC	1 cut(s)	985
BstV2I	GAAGAC	1 cut(s)	1447
BstX2I	RGATCY	3 cut(s)	811, 1744, 1848
BstYI	RGATCY	3 cut(s)	811, 1744, 1848
Bsu36I	CCTNAGG	1 cut(s)	1659
BsuRI	GGCC	4 cut(s)	701, 973, 1701, 1831
BtsCI	GGATG	5 cut(s)	92, 499, 1097, 1192, 1385
BtsI	GCAGTG	1 cut(s)	1359
BtsIMutI	CAGTG	6 cut(s)	62, 98, 1259, 1359, 1368, 1522
BveI	ACCTGC	1 cut(s)	475
Cac8I	GCNNGC	6 cut(s)	211, 369, 1012, 1800, 1965, 2010
CaiI	CAGNNNCTG	2 cut(s)	1021, 1992
CciI	TCATGA	1 cut(s)	1672
CfoI	GCGC	1 cut(s)	544
Cfr10I	RCCGGY	1 cut(s)	1291
Cfr13I	GGNCC	1 cut(s)	792
CsiI	ACCWGGT	1 cut(s)	1617
Csp6I	GTAC	6 cut(s)	109, 591, 645, 1061, 1432, 1459
CspAI	ACCGGT	1 cut(s)	1291
CspCI	CAANNNNNGTGG	2 cut(s)	1921, 1956
CviAII	CATG	9 cut(s)	118, 424, 433, 439, 633, 1150, 1234, 1603, 1673
CviQI	GTAC	6 cut(s)	109, 591, 645, 1061, 1432, 1459
DdeI	CTNAG	4 cut(s)	764, 1228, 1659, 2031
DpnI	GATC	9 cut(s)	234, 813, 911, 927, 1367, 1512, 1746, 1839, 1850
DpnII	GATC	9 cut(s)	232, 811, 909, 925, 1365, 1510, 1744, 1837, 1848
EaeI	YGGCCR	1 cut(s)	1829
Eam1104I	CTCTTC	2 cut(s)	307, 1644
EarI	CTCTTC	2 cut(s)	307, 1644
Eco32I	GATATC	1 cut(s)	1026
Eco47I	GGWCC	1 cut(s)	792
Eco47III	AGCGCT	1 cut(s)	543
Eco57I	CTGAAG	2 cut(s)	1152, 1392
Eco81I	CCTNAGG	1 cut(s)	1659
Eco88I	CYCGRG	3 cut(s)	597, 1077, 1819
Eco91I	GGTNACC	1 cut(s)	1621
EcoNI	CCTNNNNNAGG	1 cut(s)	1616
EcoO65I	GGTNACC	1 cut(s)	1621
EcoRII	CCWGG	2 cut(s)	1531, 1617
EcoRV	GATATC	1 cut(s)	1026
EcoT22I	ATGCAT	1 cut(s)	1210
FaeI	CATG	9 cut(s)	121, 427, 436, 442, 636, 1153, 1237, 1606, 1676
FalI	AAGNNNNNCTT	4 cut(s)	493, 525, 1554, 1586
FaqI	GGGAC	1 cut(s)	262
FatI	CATG	9 cut(s)	117, 423, 432, 438, 632, 1149, 1233, 1602, 1672
FauI	CCCGC	2 cut(s)	657, 883
FauNDI	CATATG	1 cut(s)	886
FbaI	TGATCA	3 cut(s)	232, 909, 925
Fnu4HI	GCNGC	1 cut(s)	999
FokI	GGATG	5 cut(s)	79, 506, 1084, 1199, 1392
Fsp4HI	GCNGC	1 cut(s)	999
FspBI	CTAG	3 cut(s)	399, 1104, 1484
GlaI	GCGC	1 cut(s)	543
GluI	GCNGC	1 cut(s)	999
HaeII	RGCGCY	1 cut(s)	545
HaeIII	GGCC	4 cut(s)	701, 973, 1701, 1831
HapII	CCGG	1 cut(s)	1292
HhaI	GCGC	1 cut(s)	544
Hin1II	CATG	9 cut(s)	121, 427, 436, 442, 636, 1153, 1237, 1606, 1676
Hin6I	GCGC	1 cut(s)	542
HinP1I	GCGC	1 cut(s)	542
HincII	GTYRAC	1 cut(s)	1467
HindII	GTYRAC	1 cut(s)	1467
HinfI	GANTC	8 cut(s)	178, 294, 916, 939, 1384, 1468, 1536, 1579
HpaII	CCGG	1 cut(s)	1292
HphI	GGTGA	5 cut(s)	699, 1234, 1351, 1607, 1615
Hpy166II	GTNNAC	7 cut(s)	109, 858, 1093, 1123, 1359, 1467, 1632
Hpy188III	TCNNGA	5 cut(s)	382, 597, 913, 1562, 1673
Hpy8I	GTNNAC	7 cut(s)	109, 858, 1093, 1123, 1359, 1467, 1632
HpyCH4III	ACNGT	7 cut(s)	102, 743, 775, 1249, 1627, 1988, 1994
HpyCH4IV	ACGT	1 cut(s)	1034
HpyF3I	CTNAG	4 cut(s)	764, 1228, 1659, 2031
HpySE526I	ACGT	1 cut(s)	1034
Hsp92II	CATG	9 cut(s)	121, 427, 436, 442, 636, 1153, 1237, 1606, 1676
HspAI	GCGC	1 cut(s)	542
Ksp22I	TGATCA	3 cut(s)	232, 909, 925
Kzo9I	GATC	9 cut(s)	232, 811, 909, 925, 1365, 1510, 1744, 1837, 1848
LmnI	GCTCC	2 cut(s)	839, 1672
Lsp1109I	GCAGC	1 cut(s)	985
LweI	GCATC	2 cut(s)	678, 792
MabI	ACCWGGT	1 cut(s)	1617
MaeI	CTAG	3 cut(s)	399, 1104, 1484
MaeII	ACGT	1 cut(s)	1034
MaeIII	GTNAC	4 cut(s)	88, 1621, 1783, 1988
MalI	GATC	9 cut(s)	234, 813, 911, 927, 1367, 1512, 1746, 1839, 1850
MbiI	CCGCTC	1 cut(s)	652
MboI	GATC	9 cut(s)	232, 811, 909, 925, 1365, 1510, 1744, 1837, 1848
MboII	GAAGA	9 cut(s)	152, 294, 712, 1312, 1452, 1462, 1631, 1778, 1886
MflI	RGATCY	3 cut(s)	811, 1744, 1848
MhlI	GDGCHC	2 cut(s)	508, 1095
MluCI	AATT	6 cut(s)	40, 375, 660, 1138, 1867, 1956
MlyI	GAGTC	2 cut(s)	172, 1462
MmeI	TCCRAC	4 cut(s)	158, 1578, 1876, 2026
Mph1103I	ATGCAT	1 cut(s)	1210
MseI	TTAA	1 cut(s)	1596
MslI	CAYNNNNRTG	3 cut(s)	437, 933, 1148
MspI	CCGG	1 cut(s)	1292
MspR9I	CCNGG	2 cut(s)	1533, 1619
Mva1269I	GAATGC	2 cut(s)	456, 2055
MvaI	CCWGG	2 cut(s)	1533, 1619
NdeI	CATATG	1 cut(s)	886
NdeII	GATC	9 cut(s)	232, 811, 909, 925, 1365, 1510, 1744, 1837, 1848
NlaIII	CATG	9 cut(s)	121, 427, 436, 442, 636, 1153, 1237, 1606, 1676
NlaIV	GGNNCC	5 cut(s)	507, 793, 1530, 1656, 2022
NmuCI	GTSAC	1 cut(s)	1621
NsiI	ATGCAT	1 cut(s)	1210
NspI	RCATGY	1 cut(s)	436
PaeR7I	CTCGAG	2 cut(s)	597, 1077
PagI	TCATGA	1 cut(s)	1672
PaqCI	CACCTGC	1 cut(s)	475
PctI	GAATGC	2 cut(s)	456, 2055
PfeI	GAWTC	6 cut(s)	294, 916, 939, 1384, 1536, 1579
PfoI	TCCNGGA	1 cut(s)	1531
PinAI	ACCGGT	1 cut(s)	1291
PkrI	GCNGC	1 cut(s)	1000
PleI	GAGTC	2 cut(s)	172, 1462
PpsI	GAGTC	2 cut(s)	172, 1462
Psp6I	CCWGG	2 cut(s)	1531, 1617
PspEI	GGTNACC	1 cut(s)	1621
PspGI	CCWGG	2 cut(s)	1531, 1617
PspN4I	GGNNCC	5 cut(s)	507, 793, 1530, 1656, 2022
PspPI	GGNCC	1 cut(s)	792
PspXI	VCTCGAGB	1 cut(s)	1077
PstI	CTGCAG	2 cut(s)	553, 1846
PstNI	CAGNNNCTG	2 cut(s)	1021, 1992
PsuI	RGATCY	3 cut(s)	811, 1744, 1848
RsaI	GTAC	6 cut(s)	110, 592, 646, 1062, 1433, 1460
RsaNI	GTAC	6 cut(s)	109, 591, 645, 1061, 1432, 1459
RseI	CAYNNNNRTG	3 cut(s)	437, 933, 1148
SaqAI	TTAA	1 cut(s)	1596
SatI	GCNGC	1 cut(s)	999
Sau3AI	GATC	9 cut(s)	232, 811, 909, 925, 1365, 1510, 1744, 1837, 1848
Sau96I	GGNCC	1 cut(s)	792
ScaI	AGTACT	1 cut(s)	1062
SchI	GAGTC	2 cut(s)	172, 1462
ScrFI	CCNGG	2 cut(s)	1533, 1619
SduI	GDGCHC	2 cut(s)	508, 1095
SexAI	ACCWGGT	1 cut(s)	1617
SfaNI	GCATC	2 cut(s)	678, 792
SfcI	CTRYAG	4 cut(s)	549, 1056, 1842, 1993
Sfr274I	CTCGAG	2 cut(s)	597, 1077
SgrAI	CRCCGGYG	1 cut(s)	1291
SinI	GGWCC	1 cut(s)	792
SlaI	CTCGAG	2 cut(s)	597, 1077
SmiMI	CAYNNNNRTG	3 cut(s)	437, 933, 1148
SmlI	CTYRAG	4 cut(s)	125, 316, 597, 1077
SmoI	CTYRAG	4 cut(s)	125, 316, 597, 1077
Sse9I	AATT	6 cut(s)	40, 375, 660, 1138, 1867, 1956
SsiI	CCGC	2 cut(s)	650, 890
SspI	AATATT	1 cut(s)	289
SspMI	CTAG	3 cut(s)	399, 1104, 1484
StyD4I	CCNGG	2 cut(s)	1531, 1617
TaaI	ACNGT	7 cut(s)	102, 743, 775, 1249, 1627, 1988, 1994
TaiI	ACGT	1 cut(s)	1037
TaqI	TCGA	6 cut(s)	598, 1078, 1642, 1758, 1906, 2040
TaqII	GACCGA	1 cut(s)	152
TasI	AATT	6 cut(s)	40, 375, 660, 1138, 1867, 1956
TatI	WGTACW	1 cut(s)	1060
TfiI	GAWTC	6 cut(s)	294, 916, 939, 1384, 1536, 1579
Tru1I	TTAA	1 cut(s)	1596
Tru9I	TTAA	1 cut(s)	1596
TscAI	CASTG	6 cut(s)	69, 105, 1266, 1359, 1375, 1522
TseFI	GTSAC	1 cut(s)	1621
TseI	GCWGC	1 cut(s)	998
Tsp45I	GTSAC	1 cut(s)	1621
TspDTI	ATGAA	6 cut(s)	255, 299, 1405, 1619, 1889, 1989
TspGWI	ACGGA	1 cut(s)	713
TspRI	CASTG	6 cut(s)	69, 105, 1266, 1359, 1375, 1522
VneI	GTGCAC	1 cut(s)	1091
VpaK11BI	GGWCC	1 cut(s)	792
XagI	CCTNNNNNAGG	1 cut(s)	1616
XapI	RAATTY	3 cut(s)	40, 375, 660
XceI	RCATGY	1 cut(s)	436
XhoI	CTCGAG	2 cut(s)	597, 1077
XspI	CTAG	3 cut(s)	399, 1104, 1484
ZrmI	AGTACT	1 cut(s)	1062
Zsp2I	ATGCAT	1 cut(s)	1210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.