Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0004541

Physical Location & Seq

Genomic Coordinates Reverse (-)

55689384 .. 55694266

Gene Structure 4883 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG392800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGGCCCTGCTGTATTGCTGCCTGAATAGCCTGATGCTCCATGATTGTTATGCACGGTGGAAAGCTGCAGCTGGTAGATGGGCTGAGACACTACTAGTTGCGTACAAGACACTGGGAGTAGTGTTTGGGGGACTTGTGACATCGCCACTCTATGTTTACTCTTTGATACCACTCAAATCTCCCACTGTAGATGACTACTTGGGCATCTACAGCATCATGTTTTGGACCCTTACTCTCATTGGAGTCGTCAAATATGCCACCATTGCTCTTAGAGCAGATGATGATGGTGAAGGTGGAACTTTCTCCCTATATTCATTACTTTGCAGGAGTATGAATATCACTGAAACTCTTTCCTCGAGACCTTCAAATCCAAAGTCATCAAGTCTTCCACGCTCTATCTATGATGACACTCAAAATCAGACTAGCATGGTAAAAGTTTTCAAGGAAAGTATAGTTGCCAGAAGGTTATTGCTTTTTATTGCTATGTTAGGCATGTGCATGCTTATTGGAGATGGTATTCTCACTCCTGCAATATCTGTTTGGTCTGCAATAGATGGAATAAGAGCACCTTTTCCATCTGTTAGTTCTTCTGTGGTGAAAGTGCTTTCTACAGCTGTGCTTGTCATTCTTTTCATGGTACAGAAATTTGGTACATCTCGAGTGAGCTTCATGTTTTCCCCGATTATGGGTGCATGGAGTTTGAGTATCATGCTCGTTGGAATATATAGCATCATTCGACACTACCCGAGCATATTTAAGGCTGTATTACCGCATTATATCTTCCGCTTCTTTTGGAGAAACGGCAAGGAAGGCTGGCTGACGCTTGGTGGCACTGTCCTTTGCATAACAGGCTCGGAGGCATTGTTTGCTGATCTTGGTCATTTCAACCGAAGCACTATTCAGATAGCTTTCTTGTTTGCAATCTACCCTTGTCTAGTAGTGACATATGCGGGGCAGACAGCATACCTAATCAATAATCAAAATGATCATAATGCTGAGTTTTATAACTTCATACCAAGTGTAATCTACTGGCCCATGTTTGTTGTATCCACATTGGCTGCCATTGTTGCCAGCCAGTCTTTGATTTCAGCCGCGTTTTCTGTAATCAAGCAAGCTGTACAGCTTGATTATTTCCCTCGAGTGAAGGTTGTGCGCACATCCCTGAGCAAAGAAGGAGAGGTTTACTCCCCTGAAGTCAACTACATTCTCATGATTCTCTCTGTTGCTGTCATACTTATATTTGGAGATGGAAAAGACATGGGGAACGCATATGGTGTTGTTGTCAGTTTGGTCATGCTCATCACCACAATATTACTGACACTGGTAATGATCATAATATGGAGAACACCACCAGTGCTGGTGGCGCTTTACTTCTCTGTCTTCTTCGTAATTGAAGGTGTTTATGTGAGTGCAGTCTTCAACAAGATCACTGAAGGTGGATGGATTCCTTTTGCCATATCCTTTATCCTTGCCTTCATTATGTTTGGTTGGTACTATGGGAGACAGAGAAAGGTAACATATGAATTGACTAATAAAATAACCTTGGACAGACTTGGAGTGCTACTATCTGATCCCAGTGTTCAAAGGGTTCCTGGATTCTGCTTCTTTTACACTAACATTCAAGATGGGCTCACTCCTGTTTTTGAACACTATGTTAAGACAATGAAATCCCTTCACCAGGTCACCGCGTTCACTACTCTTCGATATCGTTTGGTTCCTAAGGTTGCTCCTCATGAGAGGATTCTTTTCTGTAAAATTGGCCAGCAGAAAGGGGTTTATGGCTGCGTTATTCAATATGGCTACGCAGATCTCCTAAACCTTGAAGGAGATGATTTTGTTAGTCATGTTACAAATAGTTTGAGGGCACACATACAGGACAGCTCAGCTGGCCGTTTGCCATCTGATCCTGCAGAGATTCAACAAAAGTTATCTAATTTGGATGCTGCACAGATTCAACAAGAGCTATCTAATTTGGATGATCCTGCAGAGATTCAACAAGAGTTGTCTAATTTGGAAGAAGCAATCAGTGCCGGAATTGTTCATGTTCGAGGAAAGACGAGGTTTCATATTGGCAAGAGTTGTGGCTGGTTTGATAAGATTATGCTAGCATTTTATGAAGTTCTGCACAGTAATTGTAGGTCTGCTCTTCCTGCTCTCGGGGTGCCTCTGCCTCAGTGTATTGAGGTTGGAATGCTTTATGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

735

Amino Acids

81.88

Weight (kDa)

8.26

Isoelectric Point (pI)

38.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	34 - 517	2.7e-151	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	531 - 733	8e-15	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_6g30570 FvH4_6g30570
malus_domestica	MD13G1014000.v1.1
prunus_persica	Prupe.1G339400_v2.0.a1 Prupe.1G339400_v2.0.a1
pyrus_communis	pycom13g01190
rosa_chinensis	RchiOBHm_Chr2g0136991 RchiOBHm_Chr4g0445501
rosa_laevigata	RLG00000005736
rosa_multiflora	Rmu_co8256761.1_g000001 Rmu_co8447799.1_g000001 Rmu_sc0001374.1_g000059 Rmu_sc0001509.1_g000011 Rmu_sc0003556.1_g000028 Rmu_sc0010466.1_g000002
rosa_roxburghii	Rroxscaffold_2G00107790 Rroxscaffold_5G00385980
rosa_rugosa	Rorug02G0336800 Rorug04G0361800
rosa_samantha	Rh2AG385900 Rh2BG392800 Rh2CG373300 Rh2DG409000 Rh4AG423200 Rh4BG435200 Rh4DG431500
rosa_wichuraiana	Rw2G031580 Rw4G036300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1005
Acc16I	TGCGCA	1 cut(s)	1154
AccB1I	GGYRCC	1 cut(s)	2161
AccII	CGCG	2 cut(s)	1094, 1688
AciI	CCGC	5 cut(s)	770, 784, 950, 1092, 1686
AclWI	GGATC	3 cut(s)	1565, 1898, 1973
AcoI	YGGCCR	2 cut(s)	1759, 1888
AcsI	RAATTY	1 cut(s)	644
AcuI	CTGAAG	2 cut(s)	1212, 1452
AfaI	GTAC	5 cut(s)	104, 639, 652, 1119, 1493
AfiI	CCNNNNNNNGG	4 cut(s)	685, 686, 1678, 2156
AhlI	ACTAGT	1 cut(s)	94
AjnI	CCWGG	2 cut(s)	1591, 1677
Alw21I	GWGCWC	1 cut(s)	568
Alw26I	GTCTC	3 cut(s)	80, 352, 1495
AlwI	GGATC	3 cut(s)	1565, 1898, 1973
Ama87I	CYCGRG	5 cut(s)	355, 657, 745, 1137, 2156
AoxI	GGCC	4 cut(s)	3, 1031, 1759, 1888
ApeKI	GCWGC	6 cut(s)	18, 65, 68, 1058, 1782, 1943
ApoI	RAATTY	1 cut(s)	644
ArsI	GACNNNNNNTTYG	2 cut(s)	359, 391
AspLEI	GCGC	2 cut(s)	1155, 1366
AspS9I	GGNCC	3 cut(s)	4, 225, 1032
AsuHPI	GGTGA	5 cut(s)	299, 607, 1294, 1667, 1675
AsuNHI	GCTAGC	1 cut(s)	2104
AvaI	CYCGRG	5 cut(s)	355, 657, 745, 1137, 2156
AvaII	GGWCC	1 cut(s)	225
AxyI	CCTNAGG	1 cut(s)	1719
BaeGI	GKGCMC	1 cut(s)	1867
BalI	TGGCCA	1 cut(s)	1761
BanI	GGYRCC	1 cut(s)	2161
BanII	GRGCYC	1 cut(s)	1632
BbsI	GAAGAC	3 cut(s)	377, 1372, 1408
Bbv12I	GWGCWC	1 cut(s)	568
BbvI	GCAGC	6 cut(s)	5, 52, 80, 1045, 1769, 1930
BccI	CCATC	9 cut(s)	72, 278, 506, 548, 583, 1241, 1434, 1619, 1906
BceAI	ACGGC	2 cut(s)	817, 1875
BciT130I	CCWGG	2 cut(s)	1593, 1679
BciVI	GTATCC	1 cut(s)	1057
BclI	TGATCA	2 cut(s)	985, 1329
BcoDI	GTCTC	3 cut(s)	80, 352, 1495
BcuI	ACTAGT	1 cut(s)	94
BfaI	CTAG	4 cut(s)	95, 423, 935, 2105
BfmI	CTRYAG	6 cut(s)	66, 186, 208, 609, 1908, 1983
BfoI	RGCGCY	1 cut(s)	1367
BfuI	GTATCC	1 cut(s)	1057
BglII	AGATCT	1 cut(s)	1807
BisI	GCNGC	7 cut(s)	19, 66, 69, 1059, 1092, 1783, 1944
BlpI	GCTNAGC	1 cut(s)	1882
BlsI	GCNGC	7 cut(s)	20, 67, 70, 1060, 1093, 1784, 1945
Bme1390I	CCNGG	2 cut(s)	1593, 1679
Bme18I	GGWCC	1 cut(s)	225
BmeT110I	CYCGRG	5 cut(s)	355, 657, 745, 1137, 2156
BmgT120I	GGNCC	3 cut(s)	4, 225, 1032
BmiI	GGNNCC	4 cut(s)	227, 1590, 1716, 2163
BmrFI	CCNGG	2 cut(s)	1593, 1679
BmrI	ACTGGG	2 cut(s)	122, 1569
BmsI	GCATC	5 cut(s)	24, 213, 222, 738, 1930
BmtI	GCTAGC	1 cut(s)	2108
BmuI	ACTGGG	2 cut(s)	122, 1569
BpiI	GAAGAC	3 cut(s)	377, 1372, 1408
BplI	GAGNNNNNCTC	2 cut(s)	1169, 1201
Bpu10I	CCTNAGC	1 cut(s)	1163
Bpu1102I	GCTNAGC	1 cut(s)	1882
BsaI	GGTCTC	1 cut(s)	352
BsaJI	CCNNGG	1 cut(s)	1542
BsaXI	ACNNNNNCTCC	4 cut(s)	688, 718, 848, 878
Bsc4I	CCNNNNNNNGG	4 cut(s)	685, 686, 1678, 2156
Bse1I	ACTGG	6 cut(s)	117, 1034, 1075, 1326, 1352, 1575
Bse21I	CCTNAGG	1 cut(s)	1719
Bse3DI	GCAATG	1 cut(s)	261
BseBI	CCWGG	2 cut(s)	1593, 1679
BseDI	CCNNGG	1 cut(s)	1542
BseGI	GGATG	4 cut(s)	1157, 1445, 1945, 1981
BseLI	CCNNNNNNNGG	4 cut(s)	685, 686, 1678, 2156
BseMI	GCAATG	1 cut(s)	261
BseMII	CTCAG	5 cut(s)	75, 987, 1154, 1896, 2186
BseNI	ACTGG	6 cut(s)	117, 1034, 1075, 1326, 1352, 1575
BseRI	GAGGAG	1 cut(s)	1719
BseSI	GKGCMC	1 cut(s)	1867
BseXI	GCAGC	6 cut(s)	5, 52, 80, 1045, 1769, 1930
BsgI	GTGCAG	3 cut(s)	1431, 1929, 2108
Bsh1236I	CGCG	2 cut(s)	1094, 1688
BshFI	GGCC	4 cut(s)	5, 1033, 1761, 1890
BshNI	GGYRCC	1 cut(s)	2161
BsiHKAI	GWGCWC	1 cut(s)	568
BsiHKCI	CYCGRG	5 cut(s)	355, 657, 745, 1137, 2156
BsiSI	CCGG	1 cut(s)	2031
BslFI	GGGAC	1 cut(s)	144
BslI	CCNNNNNNNGG	4 cut(s)	685, 686, 1678, 2156
BsmAI	GTCTC	3 cut(s)	80, 352, 1495
BsmFI	GGGAC	1 cut(s)	144
BsmI	GAATGC	1 cut(s)	2196
BsnI	GGCC	4 cut(s)	5, 1033, 1761, 1890
Bso31I	GGTCTC	1 cut(s)	352
BsoBI	CYCGRG	5 cut(s)	355, 657, 745, 1137, 2156
Bsp1286I	GDGCHC	3 cut(s)	568, 1632, 1867
Bsp1407I	TGTACA	1 cut(s)	1117
Bsp143I	GATC	8 cut(s)	871, 985, 1329, 1425, 1570, 1807, 1903, 1978
Bsp1720I	GCTNAGC	1 cut(s)	1882
BspACI	CCGC	5 cut(s)	770, 784, 950, 1092, 1686
BspANI	GGCC	4 cut(s)	5, 1033, 1761, 1890
BspCNI	CTCAG	5 cut(s)	76, 988, 1155, 1895, 2185
BspFNI	CGCG	2 cut(s)	1094, 1688
BspHI	TCATGA	2 cut(s)	1209, 1732
BspLI	GGNNCC	4 cut(s)	227, 1590, 1716, 2163
BspMAI	CTGCAG	3 cut(s)	70, 1912, 1987
BspOI	GCTAGC	1 cut(s)	2108
BspPI	GGATC	3 cut(s)	1565, 1898, 1973
BspQI	GCTCTTC	1 cut(s)	2151
BspT107I	GGYRCC	1 cut(s)	2161
BspTNI	GGTCTC	1 cut(s)	352
BsrDI	GCAATG	1 cut(s)	261
BsrGI	TGTACA	1 cut(s)	1117
BsrI	ACTGG	6 cut(s)	117, 1034, 1075, 1326, 1352, 1575
BssECI	CCNNGG	1 cut(s)	1542
BssMI	GATC	8 cut(s)	871, 985, 1329, 1425, 1570, 1807, 1903, 1978
BssT1I	CCWWGG	1 cut(s)	1542
Bst2UI	CCWGG	2 cut(s)	1593, 1679
Bst4CI	ACNGT	4 cut(s)	57, 187, 835, 2129
Bst6I	CTCTTC	2 cut(s)	1704, 2151
BstAPI	GCANNNNNTGC	2 cut(s)	866, 2027
BstAUI	TGTACA	1 cut(s)	1117
BstC8I	GCNNGC	7 cut(s)	500, 815, 1072, 1113, 1763, 1888, 2106
BstDEI	CTNAG	7 cut(s)	84, 269, 996, 1163, 1719, 1882, 2172
BstEII	GGTNACC	1 cut(s)	1681
BstENI	CCTNNNNNAGG	1 cut(s)	1676
BstF5I	GGATG	4 cut(s)	1157, 1445, 1945, 1981
BstFNI	CGCG	2 cut(s)	1094, 1688
BstH2I	RGCGCY	1 cut(s)	1367
BstHHI	GCGC	2 cut(s)	1155, 1366
BstKTI	GATC	8 cut(s)	874, 988, 1332, 1428, 1573, 1810, 1906, 1981
BstMAI	GTCTC	3 cut(s)	80, 352, 1495
BstMBI	GATC	8 cut(s)	871, 985, 1329, 1425, 1570, 1807, 1903, 1978
BstNI	CCWGG	2 cut(s)	1593, 1679
BstNSI	RCATGY	2 cut(s)	496, 502
BstPI	GGTNACC	1 cut(s)	1681
BstSCI	CCNGG	2 cut(s)	1591, 1677
BstSFI	CTRYAG	6 cut(s)	66, 186, 208, 609, 1908, 1983
BstSLI	GKGCMC	1 cut(s)	1867
BstUI	CGCG	2 cut(s)	1094, 1688
BstV1I	GCAGC	6 cut(s)	5, 52, 80, 1045, 1769, 1930
BstV2I	GAAGAC	3 cut(s)	377, 1372, 1408
BstX2I	RGATCY	1 cut(s)	1807
BstYI	RGATCY	1 cut(s)	1807
Bsu36I	CCTNAGG	1 cut(s)	1719
BsuI	GTATCC	1 cut(s)	1057
BsuRI	GGCC	4 cut(s)	5, 1033, 1761, 1890
BtgZI	GCGATG	1 cut(s)	126
BtsCI	GGATG	4 cut(s)	1157, 1445, 1945, 1981
Cac8I	GCNNGC	7 cut(s)	500, 815, 1072, 1113, 1763, 1888, 2106
CciI	TCATGA	2 cut(s)	1209, 1732
CfoI	GCGC	2 cut(s)	1155, 1366
Cfr13I	GGNCC	3 cut(s)	4, 225, 1032
CseI	GACGC	1 cut(s)	829
CsiI	ACCWGGT	1 cut(s)	1677
Csp6I	GTAC	5 cut(s)	103, 638, 651, 1118, 1492
CspCI	CAANNNNNGTGG	2 cut(s)	2062, 2097
CviQI	GTAC	5 cut(s)	103, 638, 651, 1118, 1492
DdeI	CTNAG	7 cut(s)	84, 269, 996, 1163, 1719, 1882, 2172
DpnI	GATC	8 cut(s)	873, 987, 1331, 1427, 1572, 1809, 1905, 1980
DpnII	GATC	8 cut(s)	871, 985, 1329, 1425, 1570, 1807, 1903, 1978
EaeI	YGGCCR	2 cut(s)	1759, 1888
Eam1104I	CTCTTC	2 cut(s)	1704, 2151
EarI	CTCTTC	2 cut(s)	1704, 2151
Eco130I	CCWWGG	1 cut(s)	1542
Eco24I	GRGCYC	1 cut(s)	1632
Eco31I	GGTCTC	1 cut(s)	352
Eco32I	GATATC	1 cut(s)	1706
Eco47I	GGWCC	1 cut(s)	225
Eco57I	CTGAAG	2 cut(s)	1212, 1452
Eco81I	CCTNAGG	1 cut(s)	1719
Eco88I	CYCGRG	5 cut(s)	355, 657, 745, 1137, 2156
Eco91I	GGTNACC	1 cut(s)	1681
EcoNI	CCTNNNNNAGG	1 cut(s)	1676
EcoO65I	GGTNACC	1 cut(s)	1681
EcoRII	CCWGG	2 cut(s)	1591, 1677
EcoRV	GATATC	1 cut(s)	1706
EcoT14I	CCWWGG	1 cut(s)	1542
EcoT38I	GRGCYC	1 cut(s)	1632
ErhI	CCWWGG	1 cut(s)	1542
FalI	AAGNNNNNCTT	2 cut(s)	553, 585
FaqI	GGGAC	1 cut(s)	144
FauI	CCCGC	1 cut(s)	943
FauNDI	CATATG	3 cut(s)	946, 1270, 1519
FbaI	TGATCA	2 cut(s)	985, 1329
Fnu4HI	GCNGC	7 cut(s)	19, 66, 69, 1059, 1092, 1783, 1944
FokI	GGATG	4 cut(s)	1144, 1452, 1952, 1988
FriOI	GRGCYC	1 cut(s)	1632
Fsp4HI	GCNGC	7 cut(s)	19, 66, 69, 1059, 1092, 1783, 1944
FspAI	RTGCGCAY	1 cut(s)	1154
FspBI	CTAG	4 cut(s)	95, 423, 935, 2105
FspI	TGCGCA	1 cut(s)	1154
GlaI	GCGC	2 cut(s)	1154, 1365
GluI	GCNGC	7 cut(s)	19, 66, 69, 1059, 1092, 1783, 1944
HaeII	RGCGCY	1 cut(s)	1367
HaeIII	GGCC	4 cut(s)	5, 1033, 1761, 1890
HapII	CCGG	1 cut(s)	2031
HgaI	GACGC	1 cut(s)	829
HhaI	GCGC	2 cut(s)	1155, 1366
Hin6I	GCGC	2 cut(s)	1153, 1364
HinP1I	GCGC	2 cut(s)	1153, 1364
HincII	GTYRAC	1 cut(s)	1198
HindII	GTYRAC	1 cut(s)	1198
HinfI	GANTC	8 cut(s)	243, 1213, 1444, 1596, 1741, 1915, 1951, 1990
HpaII	CCGG	1 cut(s)	2031
HphI	GGTGA	5 cut(s)	299, 607, 1294, 1667, 1675
Hpy166II	GTNNAC	4 cut(s)	157, 1183, 1198, 1692
Hpy188I	TCNGA	5 cut(s)	420, 856, 903, 1570, 1903
Hpy188III	TCNNGA	5 cut(s)	357, 657, 1210, 1622, 1733
Hpy8I	GTNNAC	4 cut(s)	157, 1183, 1198, 1692
HpyCH4III	ACNGT	4 cut(s)	57, 187, 835, 2129
HpyF3I	CTNAG	7 cut(s)	84, 269, 996, 1163, 1719, 1882, 2172
HspAI	GCGC	2 cut(s)	1153, 1364
Ksp22I	TGATCA	2 cut(s)	985, 1329
Kzo9I	GATC	8 cut(s)	871, 985, 1329, 1425, 1570, 1807, 1903, 1978
LguI	GCTCTTC	1 cut(s)	2151
LmnI	GCTCC	2 cut(s)	42, 1732
Lsp1109I	GCAGC	6 cut(s)	5, 52, 80, 1045, 1769, 1930
LweI	GCATC	5 cut(s)	24, 213, 222, 738, 1930
MabI	ACCWGGT	1 cut(s)	1677
MaeI	CTAG	4 cut(s)	95, 423, 935, 2105
MaeIII	GTNAC	5 cut(s)	136, 940, 1513, 1681, 1846
MalI	GATC	8 cut(s)	873, 987, 1331, 1427, 1572, 1809, 1905, 1980
MboI	GATC	8 cut(s)	871, 985, 1329, 1425, 1570, 1807, 1903, 1978
MboII	GAAGA	9 cut(s)	377, 579, 772, 1372, 1375, 1408, 1691, 2027, 2138
MflI	RGATCY	1 cut(s)	1807
MhlI	GDGCHC	3 cut(s)	568, 1632, 1867
MlsI	TGGCCA	1 cut(s)	1761
MluCI	AATT	9 cut(s)	644, 1389, 1523, 1755, 1933, 1969, 2008, 2034, 2131
MluNI	TGGCCA	1 cut(s)	1761
MlyI	GAGTC	1 cut(s)	252
MmeI	TCCRAC	2 cut(s)	697, 2167
Mox20I	TGGCCA	1 cut(s)	1761
MscI	TGGCCA	1 cut(s)	1761
MseI	TTAA	2 cut(s)	756, 1656
MslI	CAYNNNNRTG	2 cut(s)	497, 1208
Msp20I	TGGCCA	1 cut(s)	1761
MspA1I	CMGCKG	3 cut(s)	71, 614, 1886
MspI	CCGG	1 cut(s)	2031
MspR9I	CCNGG	2 cut(s)	1593, 1679
Mva1269I	GAATGC	1 cut(s)	2196
MvaI	CCWGG	2 cut(s)	1593, 1679
MvnI	CGCG	2 cut(s)	1094, 1688
NdeI	CATATG	3 cut(s)	946, 1270, 1519
NdeII	GATC	8 cut(s)	871, 985, 1329, 1425, 1570, 1807, 1903, 1978
NheI	GCTAGC	1 cut(s)	2104
NlaIV	GGNNCC	4 cut(s)	227, 1590, 1716, 2163
NmuCI	GTSAC	3 cut(s)	136, 940, 1681
NsbI	TGCGCA	1 cut(s)	1154
NspI	RCATGY	2 cut(s)	496, 502
PaeI	GCATGC	1 cut(s)	502
PaeR7I	CTCGAG	3 cut(s)	355, 657, 1137
PagI	TCATGA	2 cut(s)	1209, 1732
PciSI	GCTCTTC	1 cut(s)	2151
PctI	GAATGC	1 cut(s)	2196
PfeI	GAWTC	7 cut(s)	1213, 1444, 1596, 1741, 1915, 1951, 1990
PfoI	TCCNGGA	1 cut(s)	1591
PkrI	GCNGC	7 cut(s)	20, 67, 70, 1060, 1093, 1784, 1945
PleI	GAGTC	1 cut(s)	251
PpsI	GAGTC	1 cut(s)	251
PsiI	TTATAA	1 cut(s)	1005
Psp6I	CCWGG	2 cut(s)	1591, 1677
PspEI	GGTNACC	1 cut(s)	1681
PspGI	CCWGG	2 cut(s)	1591, 1677
PspN4I	GGNNCC	4 cut(s)	227, 1590, 1716, 2163
PspPI	GGNCC	3 cut(s)	4, 225, 1032
PspXI	VCTCGAGB	1 cut(s)	1137
PstI	CTGCAG	3 cut(s)	70, 1912, 1987
PsuI	RGATCY	1 cut(s)	1807
PvuII	CAGCTG	3 cut(s)	71, 614, 1886
RsaI	GTAC	5 cut(s)	104, 639, 652, 1119, 1493
RsaNI	GTAC	5 cut(s)	103, 638, 651, 1118, 1492
RseI	CAYNNNNRTG	2 cut(s)	497, 1208
SapI	GCTCTTC	1 cut(s)	2151
SaqAI	TTAA	2 cut(s)	756, 1656
SatI	GCNGC	7 cut(s)	19, 66, 69, 1059, 1092, 1783, 1944
Sau3AI	GATC	8 cut(s)	871, 985, 1329, 1425, 1570, 1807, 1903, 1978
Sau96I	GGNCC	3 cut(s)	4, 225, 1032
SchI	GAGTC	1 cut(s)	252
ScrFI	CCNGG	2 cut(s)	1593, 1679
SduI	GDGCHC	3 cut(s)	568, 1632, 1867
SexAI	ACCWGGT	1 cut(s)	1677
SfaNI	GCATC	5 cut(s)	24, 213, 222, 738, 1930
SfcI	CTRYAG	6 cut(s)	66, 186, 208, 609, 1908, 1983
Sfr274I	CTCGAG	3 cut(s)	355, 657, 1137
SinI	GGWCC	1 cut(s)	225
SlaI	CTCGAG	3 cut(s)	355, 657, 1137
SmiMI	CAYNNNNRTG	2 cut(s)	497, 1208
SmlI	CTYRAG	3 cut(s)	355, 657, 1137
SmoI	CTYRAG	3 cut(s)	355, 657, 1137
SpeI	ACTAGT	1 cut(s)	94
SphI	GCATGC	1 cut(s)	502
Sse9I	AATT	9 cut(s)	644, 1389, 1523, 1755, 1933, 1969, 2008, 2034, 2131
SsiI	CCGC	5 cut(s)	770, 784, 950, 1092, 1686
SspI	AATATT	1 cut(s)	1311
SspMI	CTAG	4 cut(s)	95, 423, 935, 2105
StyD4I	CCNGG	2 cut(s)	1591, 1677
StyI	CCWWGG	1 cut(s)	1542
TaaI	ACNGT	4 cut(s)	57, 187, 835, 2129
TaqI	TCGA	6 cut(s)	356, 658, 736, 1138, 1702, 2047
TasI	AATT	9 cut(s)	644, 1389, 1523, 1755, 1933, 1969, 2008, 2034, 2131
TatI	WGTACW	1 cut(s)	1117
TauI	GCSGC	1 cut(s)	1094
TfiI	GAWTC	7 cut(s)	1213, 1444, 1596, 1741, 1915, 1951, 1990
Tru1I	TTAA	2 cut(s)	756, 1656
Tru9I	TTAA	2 cut(s)	756, 1656
TseFI	GTSAC	3 cut(s)	136, 940, 1681
TseI	GCWGC	6 cut(s)	18, 65, 68, 1058, 1782, 1943
Tsp45I	GTSAC	3 cut(s)	136, 940, 1681
VpaK11BI	GGWCC	1 cut(s)	225
XagI	CCTNNNNNAGG	1 cut(s)	1676
XapI	RAATTY	1 cut(s)	644
XceI	RCATGY	2 cut(s)	496, 502
XhoI	CTCGAG	3 cut(s)	355, 657, 1137
XspI	CTAG	4 cut(s)	95, 423, 935, 2105

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.