Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0004541

Physical Location & Seq

Genomic Coordinates Reverse (-)

57651758 .. 57664764

Gene Structure 13007 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG385900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGGATGAATGGAATGAGAAATGGGAGAGAGTGAAGCAGAGAAAAGTTATTCAGTCGTGCGCATTCGACTCATCTGTTACGGGATGGAAAGCTGCAGCTGGTAGATGGGCTGAGACACTACTAGTTGCGTACAAGACACTGGGAGTAGTGTTTGGGGGACTTGTGACATCGCCACTCTATGTTTACTCTTTGATACCACTCAAATCTCCCACTGTAGATGACTACTTGGGCATCTACAGCATCATGTTTTGGACCCTTACTCTCATTGGAGTCGTCAAATATGCCACCATTGCTCTTAGAGCGGATGATGATGGTGAAGGTGGAACTTTCTCCCTATATTCATTACTTTGCAGGAGTATGAATATCACTGAAACTCTTTCCTCGAGACCTTCAAATCCAAAGTCATCAAGTCTTCCACGCTCTATCTATGATGACAGTACTCAAAATCAGACTAGCATGGTAAAAGTTTTCAGGGAAAGTATAGTTGCCAGAAGGTTATTGCTTTTTATTGCTATGTTAGGCATGTGCATGCTTATTGGAGATGGTATTCTCACTCCTGCAATATCTGTTTGGTCTGCAATAGATGGAATAAGAGCACCTTTTCCATCTGTTAGTTCTTCTGTGGTGAAAGTGCTTTCTACAGCTGTGCTTGTCGTTCTTTTCATGGTACAGAAATTTGGTACATCTCGAGTGAGCTTCATGTTTTCCCCGATTATGGGTGCATGGAGTTTGAGTATCATGCTCGTTGGAATATATAGCATCATTCGACACTACCCGAGCATATTTAAGGCTGTATTACCGCATTATATCTTCCGCTTCTTTTGGAGAAACGGCAAGGAAGGCTGGCTGACGCTTGGTGGCACTGTCCTTTGCATAACAGGCTCGGAGGCATTGTTTGCTGATCTTGGTCATTTCAACCGAAGCACTATTCAGATAGCTTTCTTGTTTGCAATCTACCCTTGTCTGGTAGTGACATATGCGGGGCAGACAGCATACCTAATCAATAATCAAAATGATCATAATGCTGAGTTTTATAACTTCATACCAAGTGTAATCTACTGGCCCATGTTTGTTGTATCCACATTGGCTGCCATTGTTGCCAGCCAGTCTTTGATTTCAGCCGCGTTTTCTGTAATCAAGCAAGCTGTACAGCTTGATTATTTCCCTCGAGTGAAGGTTGTGCGCACATCCCTGAGCAAAGAAGGAGAGGTTTACTCCCCTGAAGTCAACTACATTCTCATGATTCTCTCTGTTGCTGTCATACTTATATTTGGAGATGGAAAAGACATGGGGAACGCATATGGTGTTGTTGTCAGTTTGGTCATGCTCATCACCACAATATTACTGACACTGGTAATGATCATAATATGGAGAACACCACCAGTGCTGGTGGCGCTTTACTTCTCTGTCTTCTTCGTAATTGAAGGTGTTTATGTGAGTGCAGTCTTCAACAAGATCACTGAAGGTGGATGGATTCCTTTTGCCATATCCTTTATCCTTGCCTTCATTATGTTTGGTTGGTACTATGGGAGACAGAGAAAGGTAACATATGAATTGACTAATAAAATAACCTTGGACAGACTTGGAGTGCTACTATCTGATCCCAGTGTTCAAAGGGTTCCTGGATTCTGCTTCTTTTACACTAACATTCAAGATGGGCTCACTCCTGTTTTTGAACACTATGTTAAGACAATGAAATCCCTTCACCAGGTCACCGCGTTCACTACTCTTCGATATCGTTTGGTTCCTAAGGTTGCTCCTCATGAGAGGATTCTTTTCTGTAAAATTGGCCAGCAGAAAGGGGTTTATGGCTGCGTTATTCAATATGGCTACGCAGATCTCCTAAACCTTGAAGGAGATGATTTTGTTAGTCATGTTACAAATAGTTTGAGGGCACACATACAGGACAGCTCAGCTGGCCGTTTGCTATCTGATCCTGCAGAGATTCAACAAAAGTTATCTAATTTGGATGCTGCACAGATTCAACAAGAGCTATCTAATTTGGATGATCCTGCACAGATTCAACAAGAGTTGTCTAATTTGGAAGAAGCAATCAGCGCTGGAATTGTTCATGTTCGAGGAAAGACGAGGTTTCATATTGGCAAGAGTTGTGGCTGGTTTGATAAGATTATGCTAGCATTTTATGAAGTTCTGCACAGTAATTGTAGGTCTGCTCTTCCTGCTCTCGGGGTGCCTCTGCCTCAGTGTATTGAGGTTGGAATGCTTTATGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

745

Amino Acids

83.13

Weight (kDa)

8.44

Isoelectric Point (pI)

37.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	43 - 527	3.2e-151	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	541 - 743	8.7e-15	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_6g30570 FvH4_6g30570
malus_domestica	MD13G1014000.v1.1
prunus_persica	Prupe.1G339400_v2.0.a1 Prupe.1G339400_v2.0.a1
pyrus_communis	pycom13g01190
rosa_chinensis	RchiOBHm_Chr2g0136991 RchiOBHm_Chr4g0445501
rosa_laevigata	RLG00000005736
rosa_multiflora	Rmu_co8256761.1_g000001 Rmu_co8447799.1_g000001 Rmu_sc0001374.1_g000059 Rmu_sc0001509.1_g000011 Rmu_sc0003556.1_g000028 Rmu_sc0010466.1_g000002
rosa_roxburghii	Rroxscaffold_2G00107790 Rroxscaffold_5G00385980
rosa_rugosa	Rorug02G0336800 Rorug04G0361800
rosa_samantha	Rh2AG385900 Rh2BG392800 Rh2CG373300 Rh2DG409000 Rh4AG423200 Rh4BG435200 Rh4DG431500
rosa_wichuraiana	Rw2G031580 Rw4G036300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1035
Acc16I	TGCGCA	2 cut(s)	61, 1184
AccB1I	GGYRCC	1 cut(s)	2191
AccBSI	CCGCTC	1 cut(s)	302
AccII	CGCG	2 cut(s)	1124, 1718
AciI	CCGC	6 cut(s)	302, 800, 814, 980, 1122, 1716
AclWI	GGATC	3 cut(s)	1595, 1928, 2003
AcoI	YGGCCR	2 cut(s)	1789, 1918
AcsI	RAATTY	1 cut(s)	674
AcuI	CTGAAG	2 cut(s)	1242, 1482
AfaI	GTAC	6 cut(s)	131, 439, 669, 682, 1149, 1523
AfeI	AGCGCT	1 cut(s)	2059
AfiI	CCNNNNNNNGG	5 cut(s)	715, 716, 964, 1708, 2186
AhlI	ACTAGT	1 cut(s)	121
AjnI	CCWGG	2 cut(s)	1621, 1707
Alw21I	GWGCWC	1 cut(s)	598
Alw26I	GTCTC	3 cut(s)	107, 379, 1525
AlwI	GGATC	3 cut(s)	1595, 1928, 2003
Ama87I	CYCGRG	5 cut(s)	382, 687, 775, 1167, 2186
Aor51HI	AGCGCT	1 cut(s)	2059
AoxI	GGCC	3 cut(s)	1061, 1789, 1918
ApeKI	GCWGC	5 cut(s)	92, 95, 1088, 1812, 1973
ApoI	RAATTY	1 cut(s)	674
ArsI	GACNNNNNNTTYG	2 cut(s)	386, 418
AspLEI	GCGC	4 cut(s)	62, 1185, 1396, 2060
AspS9I	GGNCC	2 cut(s)	252, 1062
AsuHPI	GGTGA	5 cut(s)	326, 637, 1324, 1697, 1705
AsuNHI	GCTAGC	1 cut(s)	2134
AvaI	CYCGRG	5 cut(s)	382, 687, 775, 1167, 2186
AvaII	GGWCC	1 cut(s)	252
AxyI	CCTNAGG	1 cut(s)	1749
BaeGI	GKGCMC	1 cut(s)	1897
BalI	TGGCCA	1 cut(s)	1791
BanI	GGYRCC	1 cut(s)	2191
BanII	GRGCYC	1 cut(s)	1662
BbsI	GAAGAC	3 cut(s)	404, 1402, 1438
Bbv12I	GWGCWC	1 cut(s)	598
BbvI	GCAGC	5 cut(s)	79, 107, 1075, 1799, 1960
BccI	CCATC	9 cut(s)	78, 99, 305, 536, 578, 613, 1271, 1464, 1649
BceAI	ACGGC	2 cut(s)	847, 1905
BciT130I	CCWGG	2 cut(s)	1623, 1709
BciVI	GTATCC	1 cut(s)	1087
BclI	TGATCA	2 cut(s)	1015, 1359
BcoDI	GTCTC	3 cut(s)	107, 379, 1525
BcuI	ACTAGT	1 cut(s)	121
BfaI	CTAG	3 cut(s)	122, 453, 2135
BfmI	CTRYAG	5 cut(s)	93, 213, 235, 639, 1938
BfoI	RGCGCY	2 cut(s)	1397, 2061
BfuI	GTATCC	1 cut(s)	1087
BglII	AGATCT	1 cut(s)	1837
BisI	GCNGC	6 cut(s)	93, 96, 1089, 1122, 1813, 1974
BlpI	GCTNAGC	1 cut(s)	1912
BlsI	GCNGC	6 cut(s)	94, 97, 1090, 1123, 1814, 1975
BmcAI	AGTACT	1 cut(s)	439
Bme1390I	CCNGG	2 cut(s)	1623, 1709
Bme18I	GGWCC	1 cut(s)	252
BmeT110I	CYCGRG	5 cut(s)	382, 687, 775, 1167, 2186
BmgT120I	GGNCC	2 cut(s)	252, 1062
BmiI	GGNNCC	4 cut(s)	254, 1620, 1746, 2193
BmrFI	CCNGG	2 cut(s)	1623, 1709
BmrI	ACTGGG	2 cut(s)	149, 1599
BmsI	GCATC	4 cut(s)	240, 249, 768, 1960
BmtI	GCTAGC	1 cut(s)	2138
BmuI	ACTGGG	2 cut(s)	149, 1599
BpiI	GAAGAC	3 cut(s)	404, 1402, 1438
BplI	GAGNNNNNCTC	2 cut(s)	1199, 1231
Bpu10I	CCTNAGC	1 cut(s)	1193
Bpu1102I	GCTNAGC	1 cut(s)	1912
BsaI	GGTCTC	1 cut(s)	379
BsaJI	CCNNGG	1 cut(s)	1572
BsaXI	ACNNNNNCTCC	4 cut(s)	718, 748, 878, 908
Bsc4I	CCNNNNNNNGG	5 cut(s)	715, 716, 964, 1708, 2186
Bse1I	ACTGG	6 cut(s)	144, 1064, 1105, 1356, 1382, 1605
Bse21I	CCTNAGG	1 cut(s)	1749
Bse3DI	GCAATG	1 cut(s)	288
BseBI	CCWGG	2 cut(s)	1623, 1709
BseDI	CCNNGG	1 cut(s)	1572
BseGI	GGATG	7 cut(s)	10, 89, 310, 1187, 1475, 1975, 2011
BseLI	CCNNNNNNNGG	5 cut(s)	715, 716, 964, 1708, 2186
BseMI	GCAATG	1 cut(s)	288
BseMII	CTCAG	5 cut(s)	102, 1017, 1184, 1926, 2216
BseNI	ACTGG	6 cut(s)	144, 1064, 1105, 1356, 1382, 1605
BseRI	GAGGAG	1 cut(s)	1749
BseSI	GKGCMC	1 cut(s)	1897
BseXI	GCAGC	5 cut(s)	79, 107, 1075, 1799, 1960
BsgI	GTGCAG	4 cut(s)	1461, 1959, 1998, 2138
Bsh1236I	CGCG	2 cut(s)	1124, 1718
BshFI	GGCC	3 cut(s)	1063, 1791, 1920
BshNI	GGYRCC	1 cut(s)	2191
BsiHKAI	GWGCWC	1 cut(s)	598
BsiHKCI	CYCGRG	5 cut(s)	382, 687, 775, 1167, 2186
BslFI	GGGAC	1 cut(s)	171
BslI	CCNNNNNNNGG	5 cut(s)	715, 716, 964, 1708, 2186
BsmAI	GTCTC	3 cut(s)	107, 379, 1525
BsmFI	GGGAC	1 cut(s)	171
BsmI	GAATGC	2 cut(s)	62, 2226
BsnI	GGCC	3 cut(s)	1063, 1791, 1920
Bso31I	GGTCTC	1 cut(s)	379
BsoBI	CYCGRG	5 cut(s)	382, 687, 775, 1167, 2186
Bsp1286I	GDGCHC	3 cut(s)	598, 1662, 1897
Bsp1407I	TGTACA	1 cut(s)	1147
Bsp143I	GATC	8 cut(s)	901, 1015, 1359, 1455, 1600, 1837, 1933, 2008
Bsp1720I	GCTNAGC	1 cut(s)	1912
BspACI	CCGC	6 cut(s)	302, 800, 814, 980, 1122, 1716
BspANI	GGCC	3 cut(s)	1063, 1791, 1920
BspCNI	CTCAG	5 cut(s)	103, 1018, 1185, 1925, 2215
BspFNI	CGCG	2 cut(s)	1124, 1718
BspHI	TCATGA	2 cut(s)	1239, 1762
BspLI	GGNNCC	4 cut(s)	254, 1620, 1746, 2193
BspMAI	CTGCAG	2 cut(s)	97, 1942
BspOI	GCTAGC	1 cut(s)	2138
BspPI	GGATC	3 cut(s)	1595, 1928, 2003
BspQI	GCTCTTC	1 cut(s)	2181
BspT107I	GGYRCC	1 cut(s)	2191
BspTNI	GGTCTC	1 cut(s)	379
BsrBI	CCGCTC	1 cut(s)	302
BsrDI	GCAATG	1 cut(s)	288
BsrGI	TGTACA	1 cut(s)	1147
BsrI	ACTGG	6 cut(s)	144, 1064, 1105, 1356, 1382, 1605
BssECI	CCNNGG	1 cut(s)	1572
BssMI	GATC	8 cut(s)	901, 1015, 1359, 1455, 1600, 1837, 1933, 2008
BssT1I	CCWWGG	1 cut(s)	1572
Bst2UI	CCWGG	2 cut(s)	1623, 1709
Bst4CI	ACNGT	4 cut(s)	214, 437, 865, 2159
Bst6I	CTCTTC	2 cut(s)	1734, 2181
BstAPI	GCANNNNNTGC	1 cut(s)	896
BstAUI	TGTACA	1 cut(s)	1147
BstC8I	GCNNGC	7 cut(s)	530, 845, 1102, 1143, 1793, 1918, 2136
BstDEI	CTNAG	7 cut(s)	111, 296, 1026, 1193, 1749, 1912, 2202
BstEII	GGTNACC	1 cut(s)	1711
BstENI	CCTNNNNNAGG	1 cut(s)	1706
BstF5I	GGATG	7 cut(s)	10, 89, 310, 1187, 1475, 1975, 2011
BstFNI	CGCG	2 cut(s)	1124, 1718
BstH2I	RGCGCY	2 cut(s)	1397, 2061
BstHHI	GCGC	4 cut(s)	62, 1185, 1396, 2060
BstKTI	GATC	8 cut(s)	904, 1018, 1362, 1458, 1603, 1840, 1936, 2011
BstMAI	GTCTC	3 cut(s)	107, 379, 1525
BstMBI	GATC	8 cut(s)	901, 1015, 1359, 1455, 1600, 1837, 1933, 2008
BstNI	CCWGG	2 cut(s)	1623, 1709
BstNSI	RCATGY	2 cut(s)	526, 532
BstPI	GGTNACC	1 cut(s)	1711
BstSCI	CCNGG	2 cut(s)	1621, 1707
BstSFI	CTRYAG	5 cut(s)	93, 213, 235, 639, 1938
BstSLI	GKGCMC	1 cut(s)	1897
BstUI	CGCG	2 cut(s)	1124, 1718
BstV1I	GCAGC	5 cut(s)	79, 107, 1075, 1799, 1960
BstV2I	GAAGAC	3 cut(s)	404, 1402, 1438
BstX2I	RGATCY	1 cut(s)	1837
BstYI	RGATCY	1 cut(s)	1837
Bsu36I	CCTNAGG	1 cut(s)	1749
BsuI	GTATCC	1 cut(s)	1087
BsuRI	GGCC	3 cut(s)	1063, 1791, 1920
BtgZI	GCGATG	1 cut(s)	153
BtsCI	GGATG	7 cut(s)	10, 89, 310, 1187, 1475, 1975, 2011
BtsIMutI	CAGTG	9 cut(s)	137, 210, 366, 861, 1349, 1389, 1458, 1612, 2210
Cac8I	GCNNGC	7 cut(s)	530, 845, 1102, 1143, 1793, 1918, 2136
CciI	TCATGA	2 cut(s)	1239, 1762
CfoI	GCGC	4 cut(s)	62, 1185, 1396, 2060
Cfr13I	GGNCC	2 cut(s)	252, 1062
CseI	GACGC	1 cut(s)	859
CsiI	ACCWGGT	1 cut(s)	1707
Csp6I	GTAC	6 cut(s)	130, 438, 668, 681, 1148, 1522
CspCI	CAANNNNNGTGG	2 cut(s)	2092, 2127
CviQI	GTAC	6 cut(s)	130, 438, 668, 681, 1148, 1522
DdeI	CTNAG	7 cut(s)	111, 296, 1026, 1193, 1749, 1912, 2202
DpnI	GATC	8 cut(s)	903, 1017, 1361, 1457, 1602, 1839, 1935, 2010
DpnII	GATC	8 cut(s)	901, 1015, 1359, 1455, 1600, 1837, 1933, 2008
EaeI	YGGCCR	2 cut(s)	1789, 1918
Eam1104I	CTCTTC	2 cut(s)	1734, 2181
EarI	CTCTTC	2 cut(s)	1734, 2181
Eco130I	CCWWGG	1 cut(s)	1572
Eco24I	GRGCYC	1 cut(s)	1662
Eco31I	GGTCTC	1 cut(s)	379
Eco32I	GATATC	1 cut(s)	1736
Eco47I	GGWCC	1 cut(s)	252
Eco47III	AGCGCT	1 cut(s)	2059
Eco57I	CTGAAG	2 cut(s)	1242, 1482
Eco81I	CCTNAGG	1 cut(s)	1749
Eco88I	CYCGRG	5 cut(s)	382, 687, 775, 1167, 2186
Eco91I	GGTNACC	1 cut(s)	1711
EcoNI	CCTNNNNNAGG	1 cut(s)	1706
EcoO65I	GGTNACC	1 cut(s)	1711
EcoRII	CCWGG	2 cut(s)	1621, 1707
EcoRV	GATATC	1 cut(s)	1736
EcoT14I	CCWWGG	1 cut(s)	1572
EcoT38I	GRGCYC	1 cut(s)	1662
ErhI	CCWWGG	1 cut(s)	1572
FalI	AAGNNNNNCTT	2 cut(s)	583, 615
FaqI	GGGAC	1 cut(s)	171
FauI	CCCGC	1 cut(s)	973
FauNDI	CATATG	3 cut(s)	976, 1300, 1549
FbaI	TGATCA	2 cut(s)	1015, 1359
Fnu4HI	GCNGC	6 cut(s)	93, 96, 1089, 1122, 1813, 1974
FokI	GGATG	7 cut(s)	17, 96, 317, 1174, 1482, 1982, 2018
FriOI	GRGCYC	1 cut(s)	1662
Fsp4HI	GCNGC	6 cut(s)	93, 96, 1089, 1122, 1813, 1974
FspAI	RTGCGCAY	2 cut(s)	61, 1184
FspBI	CTAG	3 cut(s)	122, 453, 2135
FspI	TGCGCA	2 cut(s)	61, 1184
GlaI	GCGC	4 cut(s)	61, 1184, 1395, 2059
GluI	GCNGC	6 cut(s)	93, 96, 1089, 1122, 1813, 1974
HaeII	RGCGCY	2 cut(s)	1397, 2061
HaeIII	GGCC	3 cut(s)	1063, 1791, 1920
HgaI	GACGC	1 cut(s)	859
HhaI	GCGC	4 cut(s)	62, 1185, 1396, 2060
Hin6I	GCGC	4 cut(s)	60, 1183, 1394, 2058
HinP1I	GCGC	4 cut(s)	60, 1183, 1394, 2058
HincII	GTYRAC	1 cut(s)	1228
HindII	GTYRAC	1 cut(s)	1228
HinfI	GANTC	9 cut(s)	68, 270, 1243, 1474, 1626, 1771, 1945, 1981, 2020
HphI	GGTGA	5 cut(s)	326, 637, 1324, 1697, 1705
Hpy166II	GTNNAC	4 cut(s)	184, 1213, 1228, 1722
Hpy188I	TCNGA	5 cut(s)	450, 886, 933, 1600, 1933
Hpy188III	TCNNGA	5 cut(s)	384, 687, 1240, 1652, 1763
Hpy8I	GTNNAC	4 cut(s)	184, 1213, 1228, 1722
HpyCH4III	ACNGT	4 cut(s)	214, 437, 865, 2159
HpyF3I	CTNAG	7 cut(s)	111, 296, 1026, 1193, 1749, 1912, 2202
HspAI	GCGC	4 cut(s)	60, 1183, 1394, 2058
Ksp22I	TGATCA	2 cut(s)	1015, 1359
Kzo9I	GATC	8 cut(s)	901, 1015, 1359, 1455, 1600, 1837, 1933, 2008
LguI	GCTCTTC	1 cut(s)	2181
LmnI	GCTCC	1 cut(s)	1762
Lsp1109I	GCAGC	5 cut(s)	79, 107, 1075, 1799, 1960
LweI	GCATC	4 cut(s)	240, 249, 768, 1960
MabI	ACCWGGT	1 cut(s)	1707
MaeI	CTAG	3 cut(s)	122, 453, 2135
MaeIII	GTNAC	6 cut(s)	76, 163, 970, 1543, 1711, 1876
MalI	GATC	8 cut(s)	903, 1017, 1361, 1457, 1602, 1839, 1935, 2010
MbiI	CCGCTC	1 cut(s)	302
MboI	GATC	8 cut(s)	901, 1015, 1359, 1455, 1600, 1837, 1933, 2008
MboII	GAAGA	9 cut(s)	404, 609, 802, 1402, 1405, 1438, 1721, 2057, 2168
MflI	RGATCY	1 cut(s)	1837
MhlI	GDGCHC	3 cut(s)	598, 1662, 1897
MlsI	TGGCCA	1 cut(s)	1791
MluCI	AATT	9 cut(s)	674, 1419, 1553, 1785, 1963, 1999, 2038, 2064, 2161
MluNI	TGGCCA	1 cut(s)	1791
MlyI	GAGTC	2 cut(s)	62, 279
MmeI	TCCRAC	2 cut(s)	727, 2197
Mox20I	TGGCCA	1 cut(s)	1791
MscI	TGGCCA	1 cut(s)	1791
MseI	TTAA	2 cut(s)	786, 1686
MslI	CAYNNNNRTG	2 cut(s)	527, 1238
Msp20I	TGGCCA	1 cut(s)	1791
MspA1I	CMGCKG	3 cut(s)	98, 644, 1916
MspR9I	CCNGG	2 cut(s)	1623, 1709
Mva1269I	GAATGC	2 cut(s)	62, 2226
MvaI	CCWGG	2 cut(s)	1623, 1709
MvnI	CGCG	2 cut(s)	1124, 1718
NdeI	CATATG	3 cut(s)	976, 1300, 1549
NdeII	GATC	8 cut(s)	901, 1015, 1359, 1455, 1600, 1837, 1933, 2008
NheI	GCTAGC	1 cut(s)	2134
NlaIV	GGNNCC	4 cut(s)	254, 1620, 1746, 2193
NmuCI	GTSAC	3 cut(s)	163, 970, 1711
NsbI	TGCGCA	2 cut(s)	61, 1184
NspI	RCATGY	2 cut(s)	526, 532
PaeI	GCATGC	1 cut(s)	532
PaeR7I	CTCGAG	3 cut(s)	382, 687, 1167
PagI	TCATGA	2 cut(s)	1239, 1762
PciSI	GCTCTTC	1 cut(s)	2181
PctI	GAATGC	2 cut(s)	62, 2226
PfeI	GAWTC	7 cut(s)	1243, 1474, 1626, 1771, 1945, 1981, 2020
PfoI	TCCNGGA	1 cut(s)	1621
PkrI	GCNGC	6 cut(s)	94, 97, 1090, 1123, 1814, 1975
PleI	GAGTC	2 cut(s)	62, 278
PpsI	GAGTC	2 cut(s)	62, 278
PsiI	TTATAA	1 cut(s)	1035
Psp6I	CCWGG	2 cut(s)	1621, 1707
PspEI	GGTNACC	1 cut(s)	1711
PspGI	CCWGG	2 cut(s)	1621, 1707
PspN4I	GGNNCC	4 cut(s)	254, 1620, 1746, 2193
PspPI	GGNCC	2 cut(s)	252, 1062
PspXI	VCTCGAGB	1 cut(s)	1167
PstI	CTGCAG	2 cut(s)	97, 1942
PsuI	RGATCY	1 cut(s)	1837
PvuII	CAGCTG	3 cut(s)	98, 644, 1916
RsaI	GTAC	6 cut(s)	131, 439, 669, 682, 1149, 1523
RsaNI	GTAC	6 cut(s)	130, 438, 668, 681, 1148, 1522
RseI	CAYNNNNRTG	2 cut(s)	527, 1238
SapI	GCTCTTC	1 cut(s)	2181
SaqAI	TTAA	2 cut(s)	786, 1686
SatI	GCNGC	6 cut(s)	93, 96, 1089, 1122, 1813, 1974
Sau3AI	GATC	8 cut(s)	901, 1015, 1359, 1455, 1600, 1837, 1933, 2008
Sau96I	GGNCC	2 cut(s)	252, 1062
ScaI	AGTACT	1 cut(s)	439
SchI	GAGTC	2 cut(s)	62, 279
ScrFI	CCNGG	2 cut(s)	1623, 1709
SduI	GDGCHC	3 cut(s)	598, 1662, 1897
SexAI	ACCWGGT	1 cut(s)	1707
SfaNI	GCATC	4 cut(s)	240, 249, 768, 1960
SfcI	CTRYAG	5 cut(s)	93, 213, 235, 639, 1938
Sfr274I	CTCGAG	3 cut(s)	382, 687, 1167
SinI	GGWCC	1 cut(s)	252
SlaI	CTCGAG	3 cut(s)	382, 687, 1167
SmiMI	CAYNNNNRTG	2 cut(s)	527, 1238
SmlI	CTYRAG	3 cut(s)	382, 687, 1167
SmoI	CTYRAG	3 cut(s)	382, 687, 1167
SpeI	ACTAGT	1 cut(s)	121
SphI	GCATGC	1 cut(s)	532
Sse9I	AATT	9 cut(s)	674, 1419, 1553, 1785, 1963, 1999, 2038, 2064, 2161
SsiI	CCGC	6 cut(s)	302, 800, 814, 980, 1122, 1716
SspI	AATATT	1 cut(s)	1341
SspMI	CTAG	3 cut(s)	122, 453, 2135
StyD4I	CCNGG	2 cut(s)	1621, 1707
StyI	CCWWGG	1 cut(s)	1572
TaaI	ACNGT	4 cut(s)	214, 437, 865, 2159
TaqI	TCGA	7 cut(s)	66, 383, 688, 766, 1168, 1732, 2077
TasI	AATT	9 cut(s)	674, 1419, 1553, 1785, 1963, 1999, 2038, 2064, 2161
TatI	WGTACW	2 cut(s)	437, 1147
TauI	GCSGC	1 cut(s)	1124
TfiI	GAWTC	7 cut(s)	1243, 1474, 1626, 1771, 1945, 1981, 2020
Tru1I	TTAA	2 cut(s)	786, 1686
Tru9I	TTAA	2 cut(s)	786, 1686
TscAI	CASTG	9 cut(s)	144, 217, 373, 868, 1356, 1389, 1465, 1612, 2210
TseFI	GTSAC	3 cut(s)	163, 970, 1711
TseI	GCWGC	5 cut(s)	92, 95, 1088, 1812, 1973
Tsp45I	GTSAC	3 cut(s)	163, 970, 1711
TspRI	CASTG	9 cut(s)	144, 217, 373, 868, 1356, 1389, 1465, 1612, 2210
VpaK11BI	GGWCC	1 cut(s)	252
XagI	CCTNNNNNAGG	1 cut(s)	1706
XapI	RAATTY	1 cut(s)	674
XceI	RCATGY	2 cut(s)	526, 532
XhoI	CTCGAG	3 cut(s)	382, 687, 1167
XspI	CTAG	3 cut(s)	122, 453, 2135
ZrmI	AGTACT	1 cut(s)	439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.