Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0004541

Physical Location & Seq

Genomic Coordinates Reverse (-)

59285923 .. 59292027

Gene Structure 6105 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG409000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGAGCGCAACATCTGTTCCGGTCAACATTTATTACTTATCTGTCTTGAAGGACCTAAATGGGTTTTCGGAAAGCTTGCTGCAACGTGTGTGCGCTAAGGATTCAGAATGGGGGAGTTATTGTTTCCAGTGGAAAGTTGCAGCTGGTAGATGGGCTGAGACACTACTGGTTGCGTACAAGACACTGGGAGTAGTGTTTGGGGGACTTGTGACGTCGCCACTCTATGTATACTCTTTGATGCCACTCAAATCTCCCACTGTAGATGACTACTTGGGCATCTACAGCATCATGTTTTGGACCCTTACTCTCATTGGAGTCGTTAAATATGCCACCATTGCTCTTAGAGCGGATGATGATGGTGAAGGTGGAACTTTCTCCCTATATTCATTACTTTGCAGGAGTATGAATATTACTGAAACTCTTTCCTCGAGACCTTCAAATCCAAAGTCATCAAGTCTTCCATGCTCTACCTATGATGACACTCAAAATCAGAGTAGCATGGTAAAAGTTTTCAGGGAAAGCATAGTTGCCAGAAGGTTATTGCTTTTTATTGCTATGTTAGGCATGTGCATGCTTATTGGAGATGGTATTCTCACTCCTGCAATATCTGTTTGGTCTGCAATAGATGGAATAAGAGCACCTTTTCCATCTGTTAGTTCATCTGTGGTGAAAGTGCTTTCTACAGCTGTGCTTTTCATTCTTTTCATGGTACAGAAATTTGGTACATCTCGAGTGAGCTTCATGTTTTCCCCGATTATGGGTGCATGGAGTTTGAGTATCATGCTTATTGGAATATATAGCATCATTCGACACTACCCGAGCATATTTAAGGCTGTATCACCGCATTATATCTTCCGCTTCTTTTGGAGAAACGGCAAGGAAGGCTGGCTGACGCTTGGTGGCACTGTCCTTTGCATAACAGGCTCGGAGGCATTGTTTGCTGATCTTGGTCATTTCAACCGAAGCACTATTCAGATAGCTTTCTTGTTTGCAATCTACCCTTGTCTAGTAGTGACATATGCGGGGCAGACAGCATACCTAATCAATAATCAAAATGATCATAATGCTGAGTTTTATAACTTCATACCAAGTGTAATCTACTGGCCCATGTTTGTTGTATCCACATTGGCCGCCATTGTTGCCAGCCAGTCTTTGATTTCAGCCGCATTTTCTGTGATCAAGCAAGCTGTACAGCTTGATTATTTCCCTCGAGTGAAGGTTGTGCGCACATCCCTGAGCAAAGAAGGAGAGGTTTACTCCCCTGAAGTCAACTACATTCTCATGATTCTCTCTGTTGCTGTCATACTTATATTTGGAGATGGAAAAGACATGGGGAACGCATATGGTGTTGTTGTCAGTTTGGTCATGCTCATCACCACAATATTACTGACACTGGTAATGATCATAATATGGAGAACACCACCAGTGCTGGTGGCGCTTTACTTCTCTGTCTTCTTCGTAATTGAAAGTGTTTATGTGAGTGCAGTCTTCAACAAGATAACTGAAGGTGGATGGATTCCTTTTGCCATATCCTTTATCCTTGCGATCATTATGTTTGGTTGGTACTATGGGAGACAGAGAAAGGTAACATATGAATTGACTAATAAAATAACCTTGGACAGACTTGGAGTGCTACTATCTGATCCCAGTGTTCAAATGGTTCCTGGATTCTGCTTCTTTTACACTAACATTCAAGATGGGCTCACTCCTGTTTTTGAACACTATGTTAAGACAATGAAATCCCTTCACCAGGTCACCGCGTTCACTACTCTTCAATATCGTTTGGTTCCTAAGGTTGCTCCTCATGAGAGGATTCTTTTCTGTAAAATTGGCCAGCAGAAAGGGGTTTATGGCTGCGTTATTCAATATGGCTACGCAGATCTCCTAAACCTTGAAGGAGATGATTTTGTTAGTCATGTTACAAATAGTTTGAGGGCACACATACAGGACAGCTCAGCTGGCCGTTTGCCATCTGATCCTGCAGAGATTCAACAAAAGTTATCTAATTTGGATGCTGCACAGATTCAACAAGAGCTATCTAATTTGGATGATCCTGCAGAGATTCAACAAGAGTTGTCTAATTTGGAAGAAGCAATCAGTGCTGGAATTGTTCATGTTCGAGGAAAGACGAGGTTTCATATTGGCAAGAGTTGTGGCTGGTTTGATAAGATTATGCTAGCATTTTATGAAGTTCTGCACAGTAATTGTAGGTCTGCTCTTCCTGCTCTCGGGGTGCCTCTGCCTCAGTGTATTGAGGTTGGAATGCTTTATGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

759

Amino Acids

84.48

Weight (kDa)

7.14

Isoelectric Point (pI)

40.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	58 - 541	1.6e-151	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	555 - 757	1.1e-14	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_6g30570 FvH4_6g30570
malus_domestica	MD13G1014000.v1.1
prunus_persica	Prupe.1G339400_v2.0.a1 Prupe.1G339400_v2.0.a1
pyrus_communis	pycom13g01190
rosa_chinensis	RchiOBHm_Chr2g0136991 RchiOBHm_Chr4g0445501
rosa_laevigata	RLG00000005736
rosa_multiflora	Rmu_co8256761.1_g000001 Rmu_co8447799.1_g000001 Rmu_sc0001374.1_g000059 Rmu_sc0001509.1_g000011 Rmu_sc0003556.1_g000028 Rmu_sc0010466.1_g000002
rosa_roxburghii	Rroxscaffold_2G00107790 Rroxscaffold_5G00385980
rosa_rugosa	Rorug02G0336800 Rorug04G0361800
rosa_samantha	Rh2AG385900 Rh2BG392800 Rh2CG373300 Rh2DG409000 Rh4AG423200 Rh4BG435200 Rh4DG431500
rosa_wichuraiana	Rw2G031580 Rw4G036300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1077
AatII	GACGTC	1 cut(s)	215
Acc16I	TGCGCA	1 cut(s)	1226
AccB1I	GGYRCC	1 cut(s)	2233
AccBSI	CCGCTC	1 cut(s)	347
AccI	GTMKAC	1 cut(s)	228
AccII	CGCG	1 cut(s)	1760
AciI	CCGC	7 cut(s)	347, 842, 856, 1022, 1131, 1164, 1758
AclWI	GGATC	3 cut(s)	1637, 1970, 2045
AcoI	YGGCCR	3 cut(s)	1128, 1831, 1960
AcsI	RAATTY	1 cut(s)	716
AcuI	CTGAAG	2 cut(s)	1284, 1524
AcyI	GRCGYC	1 cut(s)	212
AfaI	GTAC	5 cut(s)	176, 711, 724, 1191, 1565
AfiI	CCNNNNNNNGG	4 cut(s)	757, 758, 1750, 2228
AflIII	ACRYGT	1 cut(s)	85
AjnI	CCWGG	2 cut(s)	1663, 1749
Alw21I	GWGCWC	1 cut(s)	640
Alw26I	GTCTC	3 cut(s)	152, 424, 1567
AlwI	GGATC	3 cut(s)	1637, 1970, 2045
Ama87I	CYCGRG	5 cut(s)	427, 729, 817, 1209, 2228
AoxI	GGCC	4 cut(s)	1103, 1128, 1831, 1960
ApeKI	GCWGC	4 cut(s)	79, 140, 1854, 2015
ApoI	RAATTY	1 cut(s)	716
ArsI	GACNNNNNNTTYG	2 cut(s)	431, 463
AspLEI	GCGC	4 cut(s)	8, 95, 1227, 1438
AspS9I	GGNCC	3 cut(s)	52, 297, 1104
AsuHPI	GGTGA	6 cut(s)	371, 679, 831, 1366, 1739, 1747
AsuNHI	GCTAGC	1 cut(s)	2176
AvaI	CYCGRG	5 cut(s)	427, 729, 817, 1209, 2228
AvaII	GGWCC	2 cut(s)	52, 297
AxyI	CCTNAGG	1 cut(s)	1791
BaeGI	GKGCMC	1 cut(s)	1939
BalI	TGGCCA	1 cut(s)	1833
BanI	GGYRCC	1 cut(s)	2233
BanII	GRGCYC	1 cut(s)	1704
BbsI	GAAGAC	3 cut(s)	449, 1444, 1480
Bbv12I	GWGCWC	1 cut(s)	640
BbvI	GCAGC	4 cut(s)	66, 152, 1841, 2002
BccI	CCATC	9 cut(s)	144, 350, 578, 620, 655, 1313, 1506, 1691, 1978
BceAI	ACGGC	2 cut(s)	889, 1947
BciT130I	CCWGG	2 cut(s)	1665, 1751
BciVI	GTATCC	1 cut(s)	1129
BclI	TGATCA	3 cut(s)	1057, 1176, 1401
BcoDI	GTCTC	3 cut(s)	152, 424, 1567
BfaI	CTAG	2 cut(s)	1007, 2177
BfmI	CTRYAG	5 cut(s)	258, 280, 681, 1980, 2055
BfoI	RGCGCY	1 cut(s)	1439
BfuI	GTATCC	1 cut(s)	1129
BglII	AGATCT	1 cut(s)	1879
BisI	GCNGC	6 cut(s)	80, 141, 1131, 1164, 1855, 2016
BlpI	GCTNAGC	1 cut(s)	1954
BlsI	GCNGC	6 cut(s)	81, 142, 1132, 1165, 1856, 2017
Bme1390I	CCNGG	2 cut(s)	1665, 1751
Bme18I	GGWCC	2 cut(s)	52, 297
BmeT110I	CYCGRG	5 cut(s)	427, 729, 817, 1209, 2228
BmgT120I	GGNCC	3 cut(s)	52, 297, 1104
BmiI	GGNNCC	4 cut(s)	299, 1662, 1788, 2235
BmrFI	CCNGG	2 cut(s)	1665, 1751
BmrI	ACTGGG	2 cut(s)	194, 1641
BmsI	GCATC	5 cut(s)	228, 285, 294, 810, 2002
BmtI	GCTAGC	1 cut(s)	2180
BmuI	ACTGGG	2 cut(s)	194, 1641
BpiI	GAAGAC	3 cut(s)	449, 1444, 1480
BplI	GAGNNNNNCTC	2 cut(s)	1241, 1273
Bpu10I	CCTNAGC	2 cut(s)	96, 1235
Bpu1102I	GCTNAGC	1 cut(s)	1954
BsaHI	GRCGYC	1 cut(s)	212
BsaI	GGTCTC	1 cut(s)	424
BsaJI	CCNNGG	1 cut(s)	1614
BsaWI	WCCGGW	1 cut(s)	19
BsaXI	ACNNNNNCTCC	6 cut(s)	106, 136, 760, 790, 920, 950
Bsc4I	CCNNNNNNNGG	4 cut(s)	757, 758, 1750, 2228
Bse1I	ACTGG	8 cut(s)	127, 171, 189, 1106, 1147, 1398, 1424, 1647
Bse21I	CCTNAGG	1 cut(s)	1791
Bse3DI	GCAATG	1 cut(s)	333
BseBI	CCWGG	2 cut(s)	1665, 1751
BseDI	CCNNGG	1 cut(s)	1614
BseGI	GGATG	5 cut(s)	355, 1229, 1517, 2017, 2053
BseLI	CCNNNNNNNGG	4 cut(s)	757, 758, 1750, 2228
BseMI	GCAATG	1 cut(s)	333
BseMII	CTCAG	5 cut(s)	147, 1059, 1226, 1968, 2258
BseNI	ACTGG	8 cut(s)	127, 171, 189, 1106, 1147, 1398, 1424, 1647
BseRI	GAGGAG	1 cut(s)	1791
BseSI	GKGCMC	1 cut(s)	1939
BseXI	GCAGC	4 cut(s)	66, 152, 1841, 2002
BsgI	GTGCAG	3 cut(s)	1503, 2001, 2180
Bsh1236I	CGCG	1 cut(s)	1760
BshFI	GGCC	4 cut(s)	1105, 1130, 1833, 1962
BshNI	GGYRCC	1 cut(s)	2233
BsiHKAI	GWGCWC	1 cut(s)	640
BsiHKCI	CYCGRG	5 cut(s)	427, 729, 817, 1209, 2228
BsiSI	CCGG	1 cut(s)	20
BslFI	GGGAC	1 cut(s)	216
BslI	CCNNNNNNNGG	4 cut(s)	757, 758, 1750, 2228
BsmAI	GTCTC	3 cut(s)	152, 424, 1567
BsmFI	GGGAC	1 cut(s)	216
BsmI	GAATGC	1 cut(s)	2268
BsnI	GGCC	4 cut(s)	1105, 1130, 1833, 1962
Bso31I	GGTCTC	1 cut(s)	424
BsoBI	CYCGRG	5 cut(s)	427, 729, 817, 1209, 2228
Bsp1286I	GDGCHC	3 cut(s)	640, 1704, 1939
Bsp1407I	TGTACA	1 cut(s)	1189
Bsp143I	GATC	9 cut(s)	943, 1057, 1176, 1401, 1545, 1642, 1879, 1975, 2050
Bsp1720I	GCTNAGC	1 cut(s)	1954
BspACI	CCGC	7 cut(s)	347, 842, 856, 1022, 1131, 1164, 1758
BspANI	GGCC	4 cut(s)	1105, 1130, 1833, 1962
BspCNI	CTCAG	5 cut(s)	148, 1060, 1227, 1967, 2257
BspFNI	CGCG	1 cut(s)	1760
BspHI	TCATGA	2 cut(s)	1281, 1804
BspLI	GGNNCC	4 cut(s)	299, 1662, 1788, 2235
BspMAI	CTGCAG	2 cut(s)	1984, 2059
BspOI	GCTAGC	1 cut(s)	2180
BspPI	GGATC	3 cut(s)	1637, 1970, 2045
BspQI	GCTCTTC	1 cut(s)	2223
BspT107I	GGYRCC	1 cut(s)	2233
BspTNI	GGTCTC	1 cut(s)	424
BsrBI	CCGCTC	1 cut(s)	347
BsrDI	GCAATG	1 cut(s)	333
BsrGI	TGTACA	1 cut(s)	1189
BsrI	ACTGG	8 cut(s)	127, 171, 189, 1106, 1147, 1398, 1424, 1647
BssECI	CCNNGG	1 cut(s)	1614
BssMI	GATC	9 cut(s)	943, 1057, 1176, 1401, 1545, 1642, 1879, 1975, 2050
BssNAI	GTATAC	1 cut(s)	229
BssNI	GRCGYC	1 cut(s)	212
BssT1I	CCWWGG	1 cut(s)	1614
Bst1107I	GTATAC	1 cut(s)	229
Bst2UI	CCWGG	2 cut(s)	1665, 1751
Bst4CI	ACNGT	3 cut(s)	259, 907, 2201
Bst6I	CTCTTC	2 cut(s)	1776, 2223
BstACI	GRCGYC	1 cut(s)	212
BstAPI	GCANNNNNTGC	2 cut(s)	938, 2099
BstAUI	TGTACA	1 cut(s)	1189
BstC8I	GCNNGC	8 cut(s)	77, 572, 887, 1144, 1185, 1835, 1960, 2178
BstDEI	CTNAG	8 cut(s)	96, 156, 341, 1068, 1235, 1791, 1954, 2244
BstEII	GGTNACC	1 cut(s)	1753
BstENI	CCTNNNNNAGG	1 cut(s)	1748
BstF5I	GGATG	5 cut(s)	355, 1229, 1517, 2017, 2053
BstFNI	CGCG	1 cut(s)	1760
BstH2I	RGCGCY	1 cut(s)	1439
BstHHI	GCGC	4 cut(s)	8, 95, 1227, 1438
BstKTI	GATC	9 cut(s)	946, 1060, 1179, 1404, 1548, 1645, 1882, 1978, 2053
BstMAI	GTCTC	3 cut(s)	152, 424, 1567
BstMBI	GATC	9 cut(s)	943, 1057, 1176, 1401, 1545, 1642, 1879, 1975, 2050
BstNI	CCWGG	2 cut(s)	1665, 1751
BstNSI	RCATGY	2 cut(s)	568, 574
BstPI	GGTNACC	1 cut(s)	1753
BstSCI	CCNGG	2 cut(s)	1663, 1749
BstSFI	CTRYAG	5 cut(s)	258, 280, 681, 1980, 2055
BstSLI	GKGCMC	1 cut(s)	1939
BstUI	CGCG	1 cut(s)	1760
BstV1I	GCAGC	4 cut(s)	66, 152, 1841, 2002
BstV2I	GAAGAC	3 cut(s)	449, 1444, 1480
BstX2I	RGATCY	1 cut(s)	1879
BstYI	RGATCY	1 cut(s)	1879
BstZ17I	GTATAC	1 cut(s)	229
Bsu36I	CCTNAGG	1 cut(s)	1791
BsuI	GTATCC	1 cut(s)	1129
BsuRI	GGCC	4 cut(s)	1105, 1130, 1833, 1962
BtsCI	GGATG	5 cut(s)	355, 1229, 1517, 2017, 2053
BtsIMutI	CAGTG	9 cut(s)	134, 182, 255, 903, 1391, 1431, 1654, 2104, 2252
Cac8I	GCNNGC	8 cut(s)	77, 572, 887, 1144, 1185, 1835, 1960, 2178
CciI	TCATGA	2 cut(s)	1281, 1804
CfoI	GCGC	4 cut(s)	8, 95, 1227, 1438
Cfr13I	GGNCC	3 cut(s)	52, 297, 1104
CseI	GACGC	1 cut(s)	901
CsiI	ACCWGGT	1 cut(s)	1749
Csp6I	GTAC	5 cut(s)	175, 710, 723, 1190, 1564
CspCI	CAANNNNNGTGG	2 cut(s)	2134, 2169
CviQI	GTAC	5 cut(s)	175, 710, 723, 1190, 1564
DdeI	CTNAG	8 cut(s)	96, 156, 341, 1068, 1235, 1791, 1954, 2244
DpnI	GATC	9 cut(s)	945, 1059, 1178, 1403, 1547, 1644, 1881, 1977, 2052
DpnII	GATC	9 cut(s)	943, 1057, 1176, 1401, 1545, 1642, 1879, 1975, 2050
EaeI	YGGCCR	3 cut(s)	1128, 1831, 1960
Eam1104I	CTCTTC	2 cut(s)	1776, 2223
EarI	CTCTTC	2 cut(s)	1776, 2223
Eco130I	CCWWGG	1 cut(s)	1614
Eco24I	GRGCYC	1 cut(s)	1704
Eco31I	GGTCTC	1 cut(s)	424
Eco47I	GGWCC	2 cut(s)	52, 297
Eco57I	CTGAAG	2 cut(s)	1284, 1524
Eco81I	CCTNAGG	1 cut(s)	1791
Eco88I	CYCGRG	5 cut(s)	427, 729, 817, 1209, 2228
Eco91I	GGTNACC	1 cut(s)	1753
EcoNI	CCTNNNNNAGG	1 cut(s)	1748
EcoO109I	RGGNCCY	1 cut(s)	52
EcoO65I	GGTNACC	1 cut(s)	1753
EcoRII	CCWGG	2 cut(s)	1663, 1749
EcoT14I	CCWWGG	1 cut(s)	1614
EcoT38I	GRGCYC	1 cut(s)	1704
ErhI	CCWWGG	1 cut(s)	1614
FalI	AAGNNNNNCTT	2 cut(s)	625, 657
FaqI	GGGAC	1 cut(s)	216
FauI	CCCGC	1 cut(s)	1015
FauNDI	CATATG	3 cut(s)	1018, 1342, 1591
FbaI	TGATCA	3 cut(s)	1057, 1176, 1401
FblI	GTMKAC	1 cut(s)	228
Fnu4HI	GCNGC	6 cut(s)	80, 141, 1131, 1164, 1855, 2016
FokI	GGATG	5 cut(s)	362, 1216, 1524, 2024, 2060
FriOI	GRGCYC	1 cut(s)	1704
Fsp4HI	GCNGC	6 cut(s)	80, 141, 1131, 1164, 1855, 2016
FspAI	RTGCGCAY	1 cut(s)	1226
FspBI	CTAG	2 cut(s)	1007, 2177
FspI	TGCGCA	1 cut(s)	1226
GlaI	GCGC	4 cut(s)	7, 94, 1226, 1437
GluI	GCNGC	6 cut(s)	80, 141, 1131, 1164, 1855, 2016
HaeII	RGCGCY	1 cut(s)	1439
HaeIII	GGCC	4 cut(s)	1105, 1130, 1833, 1962
HapII	CCGG	1 cut(s)	20
HgaI	GACGC	1 cut(s)	901
HhaI	GCGC	4 cut(s)	8, 95, 1227, 1438
Hin1I	GRCGYC	1 cut(s)	212
Hin6I	GCGC	4 cut(s)	6, 93, 1225, 1436
HinP1I	GCGC	4 cut(s)	6, 93, 1225, 1436
HincII	GTYRAC	2 cut(s)	25, 1270
HindII	GTYRAC	2 cut(s)	25, 1270
HindIII	AAGCTT	1 cut(s)	73
HinfI	GANTC	9 cut(s)	101, 315, 1285, 1516, 1668, 1813, 1987, 2023, 2062
HpaII	CCGG	1 cut(s)	20
HphI	GGTGA	6 cut(s)	371, 679, 831, 1366, 1739, 1747
Hpy166II	GTNNAC	5 cut(s)	25, 229, 1255, 1270, 1764
Hpy188I	TCNGA	7 cut(s)	70, 106, 492, 928, 975, 1642, 1975
Hpy188III	TCNNGA	6 cut(s)	46, 429, 729, 1282, 1694, 1805
Hpy8I	GTNNAC	5 cut(s)	25, 229, 1255, 1270, 1764
Hpy99I	CGWCG	1 cut(s)	217
HpyCH4III	ACNGT	3 cut(s)	259, 907, 2201
HpyCH4IV	ACGT	2 cut(s)	85, 212
HpyF3I	CTNAG	8 cut(s)	96, 156, 341, 1068, 1235, 1791, 1954, 2244
HpySE526I	ACGT	2 cut(s)	85, 212
Hsp92I	GRCGYC	1 cut(s)	212
HspAI	GCGC	4 cut(s)	6, 93, 1225, 1436
Ksp22I	TGATCA	3 cut(s)	1057, 1176, 1401
Kzo9I	GATC	9 cut(s)	943, 1057, 1176, 1401, 1545, 1642, 1879, 1975, 2050
LguI	GCTCTTC	1 cut(s)	2223
LmnI	GCTCC	1 cut(s)	1804
Lsp1109I	GCAGC	4 cut(s)	66, 152, 1841, 2002
LweI	GCATC	5 cut(s)	228, 285, 294, 810, 2002
MabI	ACCWGGT	1 cut(s)	1749
MaeI	CTAG	2 cut(s)	1007, 2177
MaeII	ACGT	2 cut(s)	85, 212
MaeIII	GTNAC	5 cut(s)	208, 1012, 1585, 1753, 1918
MalI	GATC	9 cut(s)	945, 1059, 1178, 1403, 1547, 1644, 1881, 1977, 2052
MbiI	CCGCTC	1 cut(s)	347
MboI	GATC	9 cut(s)	943, 1057, 1176, 1401, 1545, 1642, 1879, 1975, 2050
MboII	GAAGA	8 cut(s)	449, 844, 1444, 1447, 1480, 1763, 2099, 2210
MflI	RGATCY	1 cut(s)	1879
MhlI	GDGCHC	3 cut(s)	640, 1704, 1939
MlsI	TGGCCA	1 cut(s)	1833
MluCI	AATT	9 cut(s)	716, 1461, 1595, 1827, 2005, 2041, 2080, 2106, 2203
MluNI	TGGCCA	1 cut(s)	1833
MlyI	GAGTC	1 cut(s)	324
MmeI	TCCRAC	1 cut(s)	2239
Mox20I	TGGCCA	1 cut(s)	1833
MscI	TGGCCA	1 cut(s)	1833
MseI	TTAA	3 cut(s)	321, 828, 1728
MslI	CAYNNNNRTG	2 cut(s)	569, 1280
Msp20I	TGGCCA	1 cut(s)	1833
MspA1I	CMGCKG	3 cut(s)	143, 686, 1958
MspI	CCGG	1 cut(s)	20
MspR9I	CCNGG	2 cut(s)	1665, 1751
Mva1269I	GAATGC	1 cut(s)	2268
MvaI	CCWGG	2 cut(s)	1665, 1751
MvnI	CGCG	1 cut(s)	1760
NdeI	CATATG	3 cut(s)	1018, 1342, 1591
NdeII	GATC	9 cut(s)	943, 1057, 1176, 1401, 1545, 1642, 1879, 1975, 2050
NheI	GCTAGC	1 cut(s)	2176
NlaIV	GGNNCC	4 cut(s)	299, 1662, 1788, 2235
NmuCI	GTSAC	3 cut(s)	208, 1012, 1753
NsbI	TGCGCA	1 cut(s)	1226
NspI	RCATGY	2 cut(s)	568, 574
PaeI	GCATGC	1 cut(s)	574
PaeR7I	CTCGAG	3 cut(s)	427, 729, 1209
PagI	TCATGA	2 cut(s)	1281, 1804
PciSI	GCTCTTC	1 cut(s)	2223
PctI	GAATGC	1 cut(s)	2268
PfeI	GAWTC	8 cut(s)	101, 1285, 1516, 1668, 1813, 1987, 2023, 2062
PfoI	TCCNGGA	1 cut(s)	1663
PkrI	GCNGC	6 cut(s)	81, 142, 1132, 1165, 1856, 2017
PleI	GAGTC	1 cut(s)	323
PpsI	GAGTC	1 cut(s)	323
PpuMI	RGGWCCY	1 cut(s)	52
PsiI	TTATAA	1 cut(s)	1077
Psp5II	RGGWCCY	1 cut(s)	52
Psp6I	CCWGG	2 cut(s)	1663, 1749
PspEI	GGTNACC	1 cut(s)	1753
PspGI	CCWGG	2 cut(s)	1663, 1749
PspN4I	GGNNCC	4 cut(s)	299, 1662, 1788, 2235
PspPI	GGNCC	3 cut(s)	52, 297, 1104
PspPPI	RGGWCCY	1 cut(s)	52
PspXI	VCTCGAGB	1 cut(s)	1209
PstI	CTGCAG	2 cut(s)	1984, 2059
PsuI	RGATCY	1 cut(s)	1879
PvuII	CAGCTG	3 cut(s)	143, 686, 1958
RsaI	GTAC	5 cut(s)	176, 711, 724, 1191, 1565
RsaNI	GTAC	5 cut(s)	175, 710, 723, 1190, 1564
RseI	CAYNNNNRTG	2 cut(s)	569, 1280
SapI	GCTCTTC	1 cut(s)	2223
SaqAI	TTAA	3 cut(s)	321, 828, 1728
SatI	GCNGC	6 cut(s)	80, 141, 1131, 1164, 1855, 2016
Sau3AI	GATC	9 cut(s)	943, 1057, 1176, 1401, 1545, 1642, 1879, 1975, 2050
Sau96I	GGNCC	3 cut(s)	52, 297, 1104
SchI	GAGTC	1 cut(s)	324
ScrFI	CCNGG	2 cut(s)	1665, 1751
SduI	GDGCHC	3 cut(s)	640, 1704, 1939
SexAI	ACCWGGT	1 cut(s)	1749
SfaNI	GCATC	5 cut(s)	228, 285, 294, 810, 2002
SfcI	CTRYAG	5 cut(s)	258, 280, 681, 1980, 2055
Sfr274I	CTCGAG	3 cut(s)	427, 729, 1209
SinI	GGWCC	2 cut(s)	52, 297
SlaI	CTCGAG	3 cut(s)	427, 729, 1209
SmiMI	CAYNNNNRTG	2 cut(s)	569, 1280
SmlI	CTYRAG	3 cut(s)	427, 729, 1209
SmoI	CTYRAG	3 cut(s)	427, 729, 1209
SphI	GCATGC	1 cut(s)	574
Sse9I	AATT	9 cut(s)	716, 1461, 1595, 1827, 2005, 2041, 2080, 2106, 2203
SsiI	CCGC	7 cut(s)	347, 842, 856, 1022, 1131, 1164, 1758
SspI	AATATT	2 cut(s)	409, 1383
SspMI	CTAG	2 cut(s)	1007, 2177
StyD4I	CCNGG	2 cut(s)	1663, 1749
StyI	CCWWGG	1 cut(s)	1614
TaaI	ACNGT	3 cut(s)	259, 907, 2201
TaiI	ACGT	2 cut(s)	88, 215
TaqI	TCGA	5 cut(s)	428, 730, 808, 1210, 2119
TasI	AATT	9 cut(s)	716, 1461, 1595, 1827, 2005, 2041, 2080, 2106, 2203
TatI	WGTACW	1 cut(s)	1189
TauI	GCSGC	2 cut(s)	1133, 1166
TfiI	GAWTC	8 cut(s)	101, 1285, 1516, 1668, 1813, 1987, 2023, 2062
Tru1I	TTAA	3 cut(s)	321, 828, 1728
Tru9I	TTAA	3 cut(s)	321, 828, 1728
TscAI	CASTG	9 cut(s)	134, 189, 262, 910, 1398, 1431, 1654, 2104, 2252
TseFI	GTSAC	3 cut(s)	208, 1012, 1753
TseI	GCWGC	4 cut(s)	79, 140, 1854, 2015
Tsp45I	GTSAC	3 cut(s)	208, 1012, 1753
TspRI	CASTG	9 cut(s)	134, 189, 262, 910, 1398, 1431, 1654, 2104, 2252
VpaK11BI	GGWCC	2 cut(s)	52, 297
XagI	CCTNNNNNAGG	1 cut(s)	1748
XapI	RAATTY	1 cut(s)	716
XceI	RCATGY	2 cut(s)	568, 574
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1654
XhoI	CTCGAG	3 cut(s)	427, 729, 1209
XmiI	GTMKAC	1 cut(s)	228
XspI	CTAG	2 cut(s)	1007, 2177
ZraI	GACGTC	1 cut(s)	213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.