Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0004541

Physical Location & Seq

Genomic Coordinates Reverse (-)

51764699 .. 51767860

Gene Structure 3162 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G031580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2193 bp

ATGCTTGTTGTTTATTTTCAAGTTCCAGTTTTGTTTGATAATGATATAAGAATCAATTTTTTATCAACTGAATTATTCAAGATTATTAGGCAGACTGGTAGATGGGCTGAGACACTACTGGTTGCGTACAAGACACTGGGAGTAGTGTTTGGGGGACTTGTGACATCGCCACTCTATGTTTACTCTTTGATGCCACTCAAATCTCCCACTGTAGATGACTACTTGGGCATCTACAGCATCATGTTTTGGACCCTTACTCTCATTGGAGTCGTTAAATATGCCACCATTGCTCTTAGAGCGGATGATGATGGTGAAGGTGGAACTTTCTCCCTATATTCATTACTTTGCAGGAGTATGGATATTACTGAAACTCTTTCCTCGAGACCTTCAAATCCAAAGTCATCAAGTCTTCCACGCTCTATCTATGATGACACTCAAAATCAGACTAGCATGGTAAAAGTTTTCAGGGAAAGCATAGTTGCCAGAAGGTTATTGCTTTTTATTGCTATGTTAGGCATGTGCATGCTTATTGGAGATGGTATTCTCACTCCTGCAATATCTGTTTGGTCTGCAATAGATGGAATAAGAGCACCTTTTCCATCTGTTAGTTCATCTGTGGTGAAAGTGCTTTCTACAGCTGTGCTTTTCATTCTTTTCATGGTACAGAAATTTGGTACATCTCGAGTGAGCTTCATGTTTTCCCCGATTATGGGTGCATGGAGTTTGAGTATCATGCTTATTGGAATATATAGCATCATTCGACACTACCCGAGCATATTTAAGGCTGTATCACCGCATTATATCTTCCGCTTCTTTTGGAGAAACGGCAAGGAAGGCTCGGAGGCATTGTTTGCAGATCTTGGTCATTTCAACCGAAGCACTATTCAGATAGCTTTCTTGTTTGCAATCTACCCTTGTCTAGTAGTGACATATGCGGGGCAGACAGCATACCTAATCAATAATCAAAATGATCATAATGCTGAGTTTTATAACTTCATACCAAGTGTTATCTACTGGCCCATGTTTGTTGTATCCACATTGGCTGCCATTGTTGCCAGCCAGTCTTTGATTTCAGCCGCGTTTTCTGTAATCAAGCAAGCTGTACAGCTTGATTATTTCCCTCGAGTGAAGGTTGTGCGCACATCCCTGAGCAAAGAAGGAGAGGTTTACTCCCCTGAAGTCAACTACATTCTCATGATTCTCTCTGTTGCTGTCATCCTTATATTTGGAGATGGAAAAGACATGGGGAACGCATATGGTGTTGTTGTCAGTTTGGTCATGCTCATCACCACAATATTACTGACACTGGTAATGATCATAATATGGAGAACACCACCAATGCTGGTGGCGCTTTACTTCTCTGTCTTCTTCGTAATTGAAGGTGTTTATGTGAGTGCAGTCTTCAACAAGATCACTGAAGGTGGATGGATTCCTTTTGCCATATCCTTTATCCTTGCCTTCATTATGTTTGGTTGGTACTATGGGAGACAGAGAAAGGTAACATATGAATTGACTCATAAAATAACCTTGGACAGACTTGGAGGGCTACTATCTGATCCCAGTGTTCAAAGGGTTCCTGGATTCTGCTTCTTTTACACTAACATTCAAGATGGGCTCACTCCTGTTTTTGAACACTATGTTAAGACAATGAAATCCCTTCACCAGGTCACCGCGTTCACTACTCTTCGATATCGTTTGGTTCCTAAGGTTGCTCCTCATGAGAGGATTCTTTTCTGTAAAATTGGCCAGCAGAAAGGGGTTTATGGCTGCGTTATTCAATATGGCTACGCAGATCTCCTAAACCTTGAAGGAGATGATTTTGTTAGTCATGTTACAAATAGTTTGCGGGCACACATACAGGACAGCTCAGCTGGCTGTTTGCCATCTGATCCTGCAGAGATTCAACAAGAGTTATCTAATTTGGATGCTGCACAGATTCAACAAGAGCTATCTAATTTGGATGATCCTGCAGAGATTCAACAAGAGTTGTCTAATTTGGAAGAAGCAATCAGTGCTGGAATTGTTCATGTTCGAGGAAAGACGAGGTTTCATATTGGCAAGAGTTGTGGCTGGTTTGATAAGATTATGCTAGCATTTTATGAAGTTCTGCACAGTAATTGTAGGTCTGCTCTTCCTGCTCTCGGGGTGCCTCTGCCTCAGTGTATTGAGGTTGGAATGATTTATGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

730

Amino Acids

81.73

Weight (kDa)

6.89

Isoelectric Point (pI)

38.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	42 - 512	4.1e-140	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	526 - 729	2.9e-15	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_4g35910 FvH4_6g30570 FvH4_6g30570
malus_domestica	MD13G1014000.v1.1
prunus_persica	Prupe.1G339400_v2.0.a1 Prupe.1G339400_v2.0.a1
pyrus_communis	pycom13g01190
rosa_chinensis	RchiOBHm_Chr2g0136991 RchiOBHm_Chr4g0445501
rosa_laevigata	RLG00000005736
rosa_multiflora	Rmu_co8256761.1_g000001 Rmu_co8447799.1_g000001 Rmu_sc0001374.1_g000059 Rmu_sc0001509.1_g000011 Rmu_sc0003556.1_g000028 Rmu_sc0010466.1_g000002
rosa_roxburghii	Rroxscaffold_2G00107790 Rroxscaffold_5G00385980
rosa_rugosa	Rorug02G0336800 Rorug04G0361800
rosa_samantha	Rh2AG385900 Rh2BG392800 Rh2CG373300 Rh2DG409000 Rh4AG423200 Rh4BG435200 Rh4DG431500
rosa_wichuraiana	Rw2G031580 Rw4G036300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	990
Acc16I	TGCGCA	1 cut(s)	1139
AccB1I	GGYRCC	1 cut(s)	2146
AccBSI	CCGCTC	1 cut(s)	299
AccII	CGCG	2 cut(s)	1079, 1673
AciI	CCGC	7 cut(s)	299, 794, 808, 935, 1077, 1671, 1846
AclWI	GGATC	3 cut(s)	1550, 1883, 1958
AcoI	YGGCCR	1 cut(s)	1744
AcsI	RAATTY	1 cut(s)	668
AcuI	CTGAAG	2 cut(s)	1197, 1437
AfaI	GTAC	5 cut(s)	128, 663, 676, 1104, 1478
AfiI	CCNNNNNNNGG	4 cut(s)	709, 710, 1663, 2141
AjnI	CCWGG	2 cut(s)	1576, 1662
AluBI	AGCT	8 cut(s)	638, 690, 893, 1100, 1108, 1866, 1871, 1948
AluI	AGCT	8 cut(s)	638, 690, 893, 1100, 1108, 1866, 1871, 1948
Alw21I	GWGCWC	1 cut(s)	592
Alw26I	GTCTC	3 cut(s)	104, 376, 1480
AlwI	GGATC	3 cut(s)	1550, 1883, 1958
Ama87I	CYCGRG	5 cut(s)	379, 681, 769, 1122, 2141
AoxI	GGCC	2 cut(s)	1016, 1744
ApeKI	GCWGC	3 cut(s)	1043, 1767, 1928
ApoI	RAATTY	1 cut(s)	668
ArsI	GACNNNNNNTTYG	2 cut(s)	383, 415
AspLEI	GCGC	2 cut(s)	1140, 1351
AspS9I	GGNCC	2 cut(s)	249, 1017
AsuHPI	GGTGA	6 cut(s)	323, 631, 783, 1279, 1652, 1660
AsuNHI	GCTAGC	1 cut(s)	2089
AvaI	CYCGRG	5 cut(s)	379, 681, 769, 1122, 2141
AvaII	GGWCC	1 cut(s)	249
AxyI	CCTNAGG	1 cut(s)	1704
BaeGI	GKGCMC	1 cut(s)	1852
BalI	TGGCCA	1 cut(s)	1746
BanI	GGYRCC	1 cut(s)	2146
BanII	GRGCYC	1 cut(s)	1617
BbsI	GAAGAC	3 cut(s)	401, 1357, 1393
Bbv12I	GWGCWC	1 cut(s)	592
BbvI	GCAGC	3 cut(s)	1030, 1754, 1915
BccI	CCATC	9 cut(s)	96, 302, 530, 572, 607, 1226, 1419, 1604, 1891
BceAI	ACGGC	1 cut(s)	841
BciT130I	CCWGG	2 cut(s)	1578, 1664
BciVI	GTATCC	1 cut(s)	1042
BclI	TGATCA	2 cut(s)	970, 1314
BcoDI	GTCTC	3 cut(s)	104, 376, 1480
BfaI	CTAG	3 cut(s)	447, 920, 2090
BfmI	CTRYAG	5 cut(s)	210, 232, 633, 1893, 1968
BfoI	RGCGCY	1 cut(s)	1352
BfuI	GTATCC	1 cut(s)	1042
BglII	AGATCT	2 cut(s)	856, 1792
BisI	GCNGC	4 cut(s)	1044, 1077, 1768, 1929
BlpI	GCTNAGC	1 cut(s)	1867
BlsI	GCNGC	4 cut(s)	1045, 1078, 1769, 1930
Bme1390I	CCNGG	2 cut(s)	1578, 1664
Bme18I	GGWCC	1 cut(s)	249
BmeT110I	CYCGRG	5 cut(s)	379, 681, 769, 1122, 2141
BmgT120I	GGNCC	2 cut(s)	249, 1017
BmiI	GGNNCC	4 cut(s)	251, 1575, 1701, 2148
BmrFI	CCNGG	2 cut(s)	1578, 1664
BmrI	ACTGGG	2 cut(s)	146, 1554
BmsI	GCATC	5 cut(s)	180, 237, 246, 762, 1915
BmtI	GCTAGC	1 cut(s)	2093
BmuI	ACTGGG	2 cut(s)	146, 1554
BpiI	GAAGAC	3 cut(s)	401, 1357, 1393
BplI	GAGNNNNNCTC	2 cut(s)	1154, 1186
Bpu10I	CCTNAGC	1 cut(s)	1148
Bpu1102I	GCTNAGC	1 cut(s)	1867
BsaI	GGTCTC	1 cut(s)	376
BsaJI	CCNNGG	1 cut(s)	1527
BsaXI	ACNNNNNCTCC	4 cut(s)	712, 742, 833, 863
Bsc4I	CCNNNNNNNGG	4 cut(s)	709, 710, 1663, 2141
Bse1I	ACTGG	8 cut(s)	26, 100, 123, 141, 1019, 1060, 1311, 1560
Bse21I	CCTNAGG	1 cut(s)	1704
Bse3DI	GCAATG	1 cut(s)	285
BseBI	CCWGG	2 cut(s)	1578, 1664
BseDI	CCNNGG	1 cut(s)	1527
BseGI	GGATG	6 cut(s)	307, 1142, 1215, 1430, 1930, 1966
BseLI	CCNNNNNNNGG	4 cut(s)	709, 710, 1663, 2141
BseMI	GCAATG	1 cut(s)	285
BseMII	CTCAG	5 cut(s)	99, 972, 1139, 1881, 2171
BseNI	ACTGG	8 cut(s)	26, 100, 123, 141, 1019, 1060, 1311, 1560
BseRI	GAGGAG	1 cut(s)	1704
BseSI	GKGCMC	1 cut(s)	1852
BseXI	GCAGC	3 cut(s)	1030, 1754, 1915
BsgI	GTGCAG	3 cut(s)	1416, 1914, 2093
Bsh1236I	CGCG	2 cut(s)	1079, 1673
BshFI	GGCC	2 cut(s)	1018, 1746
BshNI	GGYRCC	1 cut(s)	2146
BsiHKAI	GWGCWC	1 cut(s)	592
BsiHKCI	CYCGRG	5 cut(s)	379, 681, 769, 1122, 2141
BslFI	GGGAC	1 cut(s)	168
BslI	CCNNNNNNNGG	4 cut(s)	709, 710, 1663, 2141
BsmAI	GTCTC	3 cut(s)	104, 376, 1480
BsmFI	GGGAC	1 cut(s)	168
BsnI	GGCC	2 cut(s)	1018, 1746
Bso31I	GGTCTC	1 cut(s)	376
BsoBI	CYCGRG	5 cut(s)	379, 681, 769, 1122, 2141
Bsp1286I	GDGCHC	3 cut(s)	592, 1617, 1852
Bsp1407I	TGTACA	1 cut(s)	1102
Bsp143I	GATC	8 cut(s)	856, 970, 1314, 1410, 1555, 1792, 1888, 1963
Bsp1720I	GCTNAGC	1 cut(s)	1867
BspACI	CCGC	7 cut(s)	299, 794, 808, 935, 1077, 1671, 1846
BspANI	GGCC	2 cut(s)	1018, 1746
BspCNI	CTCAG	5 cut(s)	100, 973, 1140, 1880, 2170
BspFNI	CGCG	2 cut(s)	1079, 1673
BspHI	TCATGA	2 cut(s)	1194, 1717
BspLI	GGNNCC	4 cut(s)	251, 1575, 1701, 2148
BspMAI	CTGCAG	2 cut(s)	1897, 1972
BspOI	GCTAGC	1 cut(s)	2093
BspPI	GGATC	3 cut(s)	1550, 1883, 1958
BspQI	GCTCTTC	1 cut(s)	2136
BspT107I	GGYRCC	1 cut(s)	2146
BspTNI	GGTCTC	1 cut(s)	376
BsrBI	CCGCTC	1 cut(s)	299
BsrDI	GCAATG	1 cut(s)	285
BsrGI	TGTACA	1 cut(s)	1102
BsrI	ACTGG	8 cut(s)	26, 100, 123, 141, 1019, 1060, 1311, 1560
BssECI	CCNNGG	1 cut(s)	1527
BssMI	GATC	8 cut(s)	856, 970, 1314, 1410, 1555, 1792, 1888, 1963
BssT1I	CCWWGG	1 cut(s)	1527
Bst2UI	CCWGG	2 cut(s)	1578, 1664
Bst4CI	ACNGT	2 cut(s)	211, 2114
Bst6I	CTCTTC	2 cut(s)	1689, 2136
BstAPI	GCANNNNNTGC	2 cut(s)	851, 2012
BstAUI	TGTACA	1 cut(s)	1102
BstC8I	GCNNGC	7 cut(s)	524, 1057, 1098, 1748, 1848, 1873, 2091
BstDEI	CTNAG	7 cut(s)	108, 293, 981, 1148, 1704, 1867, 2157
BstEII	GGTNACC	1 cut(s)	1666
BstENI	CCTNNNNNAGG	1 cut(s)	1661
BstF5I	GGATG	6 cut(s)	307, 1142, 1215, 1430, 1930, 1966
BstFNI	CGCG	2 cut(s)	1079, 1673
BstH2I	RGCGCY	1 cut(s)	1352
BstHHI	GCGC	2 cut(s)	1140, 1351
BstKTI	GATC	8 cut(s)	859, 973, 1317, 1413, 1558, 1795, 1891, 1966
BstMAI	GTCTC	3 cut(s)	104, 376, 1480
BstMBI	GATC	8 cut(s)	856, 970, 1314, 1410, 1555, 1792, 1888, 1963
BstNI	CCWGG	2 cut(s)	1578, 1664
BstNSI	RCATGY	2 cut(s)	520, 526
BstPI	GGTNACC	1 cut(s)	1666
BstSCI	CCNGG	2 cut(s)	1576, 1662
BstSFI	CTRYAG	5 cut(s)	210, 232, 633, 1893, 1968
BstSLI	GKGCMC	1 cut(s)	1852
BstUI	CGCG	2 cut(s)	1079, 1673
BstV1I	GCAGC	3 cut(s)	1030, 1754, 1915
BstV2I	GAAGAC	3 cut(s)	401, 1357, 1393
BstX2I	RGATCY	2 cut(s)	856, 1792
BstYI	RGATCY	2 cut(s)	856, 1792
Bsu36I	CCTNAGG	1 cut(s)	1704
BsuI	GTATCC	1 cut(s)	1042
BsuRI	GGCC	2 cut(s)	1018, 1746
BtgZI	GCGATG	1 cut(s)	150
BtsCI	GGATG	6 cut(s)	307, 1142, 1215, 1430, 1930, 1966
BtsIMutI	CAGTG	7 cut(s)	134, 207, 1304, 1413, 1567, 2017, 2165
Cac8I	GCNNGC	7 cut(s)	524, 1057, 1098, 1748, 1848, 1873, 2091
CciI	TCATGA	2 cut(s)	1194, 1717
CfoI	GCGC	2 cut(s)	1140, 1351
Cfr13I	GGNCC	2 cut(s)	249, 1017
CsiI	ACCWGGT	1 cut(s)	1662
Csp6I	GTAC	5 cut(s)	127, 662, 675, 1103, 1477
CspCI	CAANNNNNGTGG	4 cut(s)	1326, 1361, 2047, 2082
CviQI	GTAC	5 cut(s)	127, 662, 675, 1103, 1477
DdeI	CTNAG	7 cut(s)	108, 293, 981, 1148, 1704, 1867, 2157
DpnI	GATC	8 cut(s)	858, 972, 1316, 1412, 1557, 1794, 1890, 1965
DpnII	GATC	8 cut(s)	856, 970, 1314, 1410, 1555, 1792, 1888, 1963
EaeI	YGGCCR	1 cut(s)	1744
Eam1104I	CTCTTC	2 cut(s)	1689, 2136
EarI	CTCTTC	2 cut(s)	1689, 2136
Eco130I	CCWWGG	1 cut(s)	1527
Eco24I	GRGCYC	1 cut(s)	1617
Eco31I	GGTCTC	1 cut(s)	376
Eco32I	GATATC	1 cut(s)	1691
Eco47I	GGWCC	1 cut(s)	249
Eco57I	CTGAAG	2 cut(s)	1197, 1437
Eco81I	CCTNAGG	1 cut(s)	1704
Eco88I	CYCGRG	5 cut(s)	379, 681, 769, 1122, 2141
Eco91I	GGTNACC	1 cut(s)	1666
EcoNI	CCTNNNNNAGG	1 cut(s)	1661
EcoO65I	GGTNACC	1 cut(s)	1666
EcoRII	CCWGG	2 cut(s)	1576, 1662
EcoRV	GATATC	1 cut(s)	1691
EcoT14I	CCWWGG	1 cut(s)	1527
EcoT38I	GRGCYC	1 cut(s)	1617
ErhI	CCWWGG	1 cut(s)	1527
FalI	AAGNNNNNCTT	2 cut(s)	577, 609
FaqI	GGGAC	1 cut(s)	168
FauI	CCCGC	2 cut(s)	928, 1839
FauNDI	CATATG	3 cut(s)	931, 1255, 1504
FbaI	TGATCA	2 cut(s)	970, 1314
Fnu4HI	GCNGC	4 cut(s)	1044, 1077, 1768, 1929
FokI	GGATG	6 cut(s)	314, 1129, 1202, 1437, 1937, 1973
FriOI	GRGCYC	1 cut(s)	1617
Fsp4HI	GCNGC	4 cut(s)	1044, 1077, 1768, 1929
FspAI	RTGCGCAY	1 cut(s)	1139
FspBI	CTAG	3 cut(s)	447, 920, 2090
FspI	TGCGCA	1 cut(s)	1139
GlaI	GCGC	2 cut(s)	1139, 1350
GluI	GCNGC	4 cut(s)	1044, 1077, 1768, 1929
HaeII	RGCGCY	1 cut(s)	1352
HaeIII	GGCC	2 cut(s)	1018, 1746
HhaI	GCGC	2 cut(s)	1140, 1351
Hin6I	GCGC	2 cut(s)	1138, 1349
HinP1I	GCGC	2 cut(s)	1138, 1349
HincII	GTYRAC	1 cut(s)	1183
HindII	GTYRAC	1 cut(s)	1183
HphI	GGTGA	6 cut(s)	323, 631, 783, 1279, 1652, 1660
Hpy166II	GTNNAC	4 cut(s)	181, 1168, 1183, 1677
Hpy188I	TCNGA	5 cut(s)	444, 841, 888, 1555, 1888
Hpy188III	TCNNGA	6 cut(s)	79, 381, 681, 1195, 1607, 1718
Hpy8I	GTNNAC	4 cut(s)	181, 1168, 1183, 1677
HpyCH4III	ACNGT	2 cut(s)	211, 2114
HpyF3I	CTNAG	7 cut(s)	108, 293, 981, 1148, 1704, 1867, 2157
HspAI	GCGC	2 cut(s)	1138, 1349
Ksp22I	TGATCA	2 cut(s)	970, 1314
Kzo9I	GATC	8 cut(s)	856, 970, 1314, 1410, 1555, 1792, 1888, 1963
LguI	GCTCTTC	1 cut(s)	2136
LmnI	GCTCC	1 cut(s)	1717
Lsp1109I	GCAGC	3 cut(s)	1030, 1754, 1915
LweI	GCATC	5 cut(s)	180, 237, 246, 762, 1915
MabI	ACCWGGT	1 cut(s)	1662
MaeI	CTAG	3 cut(s)	447, 920, 2090
MaeIII	GTNAC	5 cut(s)	160, 925, 1498, 1666, 1831
MalI	GATC	8 cut(s)	858, 972, 1316, 1412, 1557, 1794, 1890, 1965
MbiI	CCGCTC	1 cut(s)	299
MboI	GATC	8 cut(s)	856, 970, 1314, 1410, 1555, 1792, 1888, 1963
MboII	GAAGA	8 cut(s)	401, 796, 1357, 1360, 1393, 1676, 2012, 2123
MflI	RGATCY	2 cut(s)	856, 1792
MhlI	GDGCHC	3 cut(s)	592, 1617, 1852
MlsI	TGGCCA	1 cut(s)	1746
MluNI	TGGCCA	1 cut(s)	1746
MlyI	GAGTC	2 cut(s)	276, 1507
MmeI	TCCRAC	1 cut(s)	2152
Mox20I	TGGCCA	1 cut(s)	1746
MscI	TGGCCA	1 cut(s)	1746
MseI	TTAA	3 cut(s)	273, 780, 1641
MslI	CAYNNNNRTG	2 cut(s)	521, 1193
Msp20I	TGGCCA	1 cut(s)	1746
MspA1I	CMGCKG	2 cut(s)	638, 1871
MspR9I	CCNGG	2 cut(s)	1578, 1664
MvaI	CCWGG	2 cut(s)	1578, 1664
MvnI	CGCG	2 cut(s)	1079, 1673
NdeI	CATATG	3 cut(s)	931, 1255, 1504
NdeII	GATC	8 cut(s)	856, 970, 1314, 1410, 1555, 1792, 1888, 1963
NheI	GCTAGC	1 cut(s)	2089
NlaIV	GGNNCC	4 cut(s)	251, 1575, 1701, 2148
NmuCI	GTSAC	3 cut(s)	160, 925, 1666
NsbI	TGCGCA	1 cut(s)	1139
NspI	RCATGY	2 cut(s)	520, 526
PaeI	GCATGC	1 cut(s)	526
PaeR7I	CTCGAG	3 cut(s)	379, 681, 1122
PagI	TCATGA	2 cut(s)	1194, 1717
PciSI	GCTCTTC	1 cut(s)	2136
PfeI	GAWTC	8 cut(s)	51, 1198, 1429, 1581, 1726, 1900, 1936, 1975
PfoI	TCCNGGA	1 cut(s)	1576
PkrI	GCNGC	4 cut(s)	1045, 1078, 1769, 1930
PleI	GAGTC	2 cut(s)	275, 1507
PpsI	GAGTC	2 cut(s)	275, 1507
PsiI	TTATAA	1 cut(s)	990
Psp6I	CCWGG	2 cut(s)	1576, 1662
PspEI	GGTNACC	1 cut(s)	1666
PspGI	CCWGG	2 cut(s)	1576, 1662
PspN4I	GGNNCC	4 cut(s)	251, 1575, 1701, 2148
PspPI	GGNCC	2 cut(s)	249, 1017
PspXI	VCTCGAGB	1 cut(s)	1122
PstI	CTGCAG	2 cut(s)	1897, 1972
PsuI	RGATCY	2 cut(s)	856, 1792
PvuII	CAGCTG	2 cut(s)	638, 1871
RsaI	GTAC	5 cut(s)	128, 663, 676, 1104, 1478
RsaNI	GTAC	5 cut(s)	127, 662, 675, 1103, 1477
RseI	CAYNNNNRTG	2 cut(s)	521, 1193
SapI	GCTCTTC	1 cut(s)	2136
SaqAI	TTAA	3 cut(s)	273, 780, 1641
SatI	GCNGC	4 cut(s)	1044, 1077, 1768, 1929
Sau3AI	GATC	8 cut(s)	856, 970, 1314, 1410, 1555, 1792, 1888, 1963
Sau96I	GGNCC	2 cut(s)	249, 1017
SchI	GAGTC	2 cut(s)	276, 1507
ScrFI	CCNGG	2 cut(s)	1578, 1664
SduI	GDGCHC	3 cut(s)	592, 1617, 1852
SexAI	ACCWGGT	1 cut(s)	1662
SfaNI	GCATC	5 cut(s)	180, 237, 246, 762, 1915
SfcI	CTRYAG	5 cut(s)	210, 232, 633, 1893, 1968
Sfr274I	CTCGAG	3 cut(s)	379, 681, 1122
SinI	GGWCC	1 cut(s)	249
SlaI	CTCGAG	3 cut(s)	379, 681, 1122
SmiMI	CAYNNNNRTG	2 cut(s)	521, 1193
SmlI	CTYRAG	3 cut(s)	379, 681, 1122
SmoI	CTYRAG	3 cut(s)	379, 681, 1122
SphI	GCATGC	1 cut(s)	526
SsiI	CCGC	7 cut(s)	299, 794, 808, 935, 1077, 1671, 1846
SspI	AATATT	1 cut(s)	1296
SspMI	CTAG	3 cut(s)	447, 920, 2090
StyD4I	CCNGG	2 cut(s)	1576, 1662
StyI	CCWWGG	1 cut(s)	1527
TaaI	ACNGT	2 cut(s)	211, 2114
TaqI	TCGA	6 cut(s)	380, 682, 760, 1123, 1687, 2032
TatI	WGTACW	1 cut(s)	1102
TauI	GCSGC	1 cut(s)	1079
TfiI	GAWTC	8 cut(s)	51, 1198, 1429, 1581, 1726, 1900, 1936, 1975
Tru1I	TTAA	3 cut(s)	273, 780, 1641
Tru9I	TTAA	3 cut(s)	273, 780, 1641
TscAI	CASTG	7 cut(s)	141, 214, 1311, 1420, 1567, 2017, 2165
TseFI	GTSAC	3 cut(s)	160, 925, 1666
TseI	GCWGC	3 cut(s)	1043, 1767, 1928
Tsp45I	GTSAC	3 cut(s)	160, 925, 1666
TspRI	CASTG	7 cut(s)	141, 214, 1311, 1420, 1567, 2017, 2165
VpaK11BI	GGWCC	1 cut(s)	249
XagI	CCTNNNNNAGG	1 cut(s)	1661
XapI	RAATTY	1 cut(s)	668
XceI	RCATGY	2 cut(s)	520, 526
XhoI	CTCGAG	3 cut(s)	379, 681, 1122
XspI	CTAG	3 cut(s)	447, 920, 2090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.