MD13G1015500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013160
Physical Location & Seq
Forward (+)
987581 .. 988850
1270 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1015500.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGGATTTTCATGTGAGGATGATTTTGACGGAAAGGAAGGCGAAGCTCCACTTTTATATGAAACGAGGGAGAATCAACACAGAAGCAGCAGGTGCATGGATGTTCATAATCATGACCATCATATGCAGGTTGAAGTCGACTTCGAGTTTTGGCCGGTGGAACATCCTCTTGAGCCACCTGATGAGGATCGCCCTGTCAAATGCCCGATGCCCGACTCTTCTGTCATTAATGGAGGAGGGAGGAGGGAAAAAAGGCTTTCAGAGAGCAATAGCATGCGTAAGAGGACAGAAGCATCGTCTGCGCCTGGAGCAGCTTATAGCAATCAAGGATTAATGAATCAGACGGTAGCGGCAGTGGCAACGCCGCCTCCTGTTCGAGCAGTGCGTAAGAGGCACCACCACACTCTCACCCGCGGCGACCATATGATATCACCTCTGAGAAGGATGCCACCTCTTCCTTCTCTCCCAACTCAGAGCATTACCGTCTTTCAAATGCTCCAGCAGTTCGACAAGTTCGACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.78

Weight (kDa)

6.19

Isoelectric Point (pI)

65.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01913
fragaria_vesca FvH4_4g26140 FvH4_4g26140
malus_domestica MD13G1015500.v1.1 MD16G1013000.v1.1
prunus_persica Prupe.1G338300_v2.0.a1 Prupe.1G338300_v2.0.a1
pyrus_communis pycom13g01310 pycom16g01220
rosa_laevigata RLG00000006736
rosa_multiflora Rmu_sc0000312.1_g000026
rosa_roxburghii Rroxscaffold_5G00375150
rosa_rugosa Rorug04G0271100.1
rosa_samantha Rh4AG325400 Rh4BG333200 Rh4CG348400 Rh4DG329800
rosa_wichuraiana Rw4G028180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 81
AasI GACNNNNNNGTC 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 81, 118
AccB1I GGYRCC 1 cut(s) 393
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 414
AciI CCGC 4 cut(s) 350, 365, 412, 414
AclWI GGATC 1 cut(s) 195
AcoI YGGCCR 1 cut(s) 152
AgsI TTSAA 2 cut(s) 134, 491
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 2 cut(s) 47, 314
AluI AGCT 2 cut(s) 47, 314
AlwI GGATC 1 cut(s) 195
AoxI GGCC 1 cut(s) 152
ApeKI GCWGC 2 cut(s) 87, 311
AseI ATTAAT 2 cut(s) 228, 332
AspLEI GCGC 1 cut(s) 304
AsuHPI GGTGA 2 cut(s) 400, 423
BanI GGYRCC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 99, 323
BccI CCATC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 306
BfuAI ACCTGC 2 cut(s) 81, 118
BisI GCNGC 5 cut(s) 88, 312, 351, 365, 415
BlsI GCNGC 5 cut(s) 89, 313, 352, 366, 416
Bme1390I CCNGG 1 cut(s) 306
BmiI GGNNCC 1 cut(s) 395
BmrFI CCNGG 1 cut(s) 306
BmsI GCATC 3 cut(s) 198, 302, 435
BpmI CTGGAG 2 cut(s) 327, 482
BpuEI CTTGAG 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 352, 382
Bse118I RCCGGY 1 cut(s) 154
Bse8I GATNNNNATC 1 cut(s) 186
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 1 cut(s) 412
BseGI GGATG 4 cut(s) 25, 106, 163, 450
BseJI GATNNNNATC 1 cut(s) 186
BseMII CTCAG 2 cut(s) 428, 485
BseRI GAGGAG 2 cut(s) 249, 256
BseXI GCAGC 2 cut(s) 99, 323
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 1 cut(s) 154
BshNI GGYRCC 1 cut(s) 393
BsiSI CCGG 1 cut(s) 155
BsnI GGCC 1 cut(s) 154
Bsp143I GATC 1 cut(s) 187
BspACI CCGC 4 cut(s) 350, 365, 412, 414
BspANI GGCC 1 cut(s) 154
BspCNI CTCAG 2 cut(s) 429, 484
BspFNI CGCG 1 cut(s) 414
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 395
BspMI ACCTGC 2 cut(s) 81, 118
BspPI GGATC 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 412
BssMI GATC 1 cut(s) 187
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 346, 484
Bst6I CTCTTC 2 cut(s) 223, 459
BstAPI GCANNNNNTGC 2 cut(s) 93, 299
BstC8I GCNNGC 1 cut(s) 275
BstDEI CTNAG 2 cut(s) 437, 471
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 4 cut(s) 25, 106, 163, 450
BstFNI CGCG 1 cut(s) 414
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 1 cut(s) 190
BstMBI GATC 1 cut(s) 187
BstMWI GCNNNNNNNGC 5 cut(s) 93, 299, 308, 356, 391
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 304
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 2 cut(s) 99, 323
BsuRI GGCC 1 cut(s) 154
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 4 cut(s) 25, 106, 163, 450
BtsI GCAGTG 2 cut(s) 360, 387
BtsIMutI CAGTG 2 cut(s) 360, 387
BveI ACCTGC 2 cut(s) 81, 118
Cac8I GCNNGC 1 cut(s) 275
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 304
Cfr10I RCCGGY 1 cut(s) 154
Cfr42I CCGCGG 1 cut(s) 415
CviAII CATG 4 cut(s) 12, 97, 113, 274
CviJI RGCY 5 cut(s) 47, 154, 175, 256, 314
CviKI_1 RGCY 5 cut(s) 47, 154, 175, 256, 314
DdeI CTNAG 2 cut(s) 437, 471
DpnI GATC 1 cut(s) 189
DpnII GATC 1 cut(s) 187
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 152
Eam1104I CTCTTC 2 cut(s) 223, 459
EarI CTCTTC 2 cut(s) 223, 459
Eco32I GATATC 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 304
EcoRV GATATC 1 cut(s) 429
FaeI CATG 4 cut(s) 15, 100, 116, 277
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FatI CATG 4 cut(s) 11, 96, 112, 273
FauI CCCGC 1 cut(s) 419
FauNDI CATATG 2 cut(s) 123, 423
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 5 cut(s) 88, 312, 351, 365, 415
FokI GGATG 4 cut(s) 32, 113, 150, 457
Fsp4HI GCNGC 5 cut(s) 88, 312, 351, 365, 415
GlaI GCGC 1 cut(s) 303
GluI GCNGC 5 cut(s) 88, 312, 351, 365, 415
GsuI CTGGAG 2 cut(s) 327, 482
HaeIII GGCC 1 cut(s) 154
HapII CCGG 1 cut(s) 155
HhaI GCGC 1 cut(s) 304
Hin1II CATG 4 cut(s) 15, 100, 116, 277
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 4 cut(s) 73, 215, 337, 518
HpaII CCGG 1 cut(s) 155
HphI GGTGA 2 cut(s) 400, 423
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 4 cut(s) 262, 342, 438, 474
Hpy188III TCNNGA 3 cut(s) 113, 170, 522
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 32, 435, 468
HpyCH4III ACNGT 2 cut(s) 346, 484
HpyCH4V TGCA 2 cut(s) 96, 127
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 299, 308, 356, 391
HpyF3I CTNAG 2 cut(s) 437, 471
Hsp92II CATG 4 cut(s) 15, 100, 116, 277
HspAI GCGC 1 cut(s) 302
KspI CCGCGG 1 cut(s) 415
Kzo9I GATC 1 cut(s) 187
LmnI GCTCC 3 cut(s) 52, 308, 501
LpnPI CCDG 9 cut(s) 76, 113, 168, 192, 207, 291, 318, 384, 512
Lsp1109I GCAGC 2 cut(s) 99, 323
LweI GCATC 3 cut(s) 198, 302, 435
MalI GATC 1 cut(s) 189
MboI GATC 1 cut(s) 187
MboII GAAGA 2 cut(s) 210, 446
MlyI GAGTC 2 cut(s) 209, 512
MseI TTAA 2 cut(s) 228, 332
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 414
MspI CCGG 1 cut(s) 155
MspR9I CCNGG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 306
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 5 cut(s) 93, 299, 308, 356, 391
NdeI CATATG 2 cut(s) 123, 423
NdeII GATC 1 cut(s) 187
NlaIII CATG 4 cut(s) 15, 100, 116, 277
NlaIV GGNNCC 1 cut(s) 395
NspI RCATGY 1 cut(s) 277
PaeI GCATGC 1 cut(s) 277
PagI TCATGA 1 cut(s) 112
PaqCI CACCTGC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 2 cut(s) 382, 513
PfeI GAWTC 2 cut(s) 73, 337
PkrI GCNGC 5 cut(s) 89, 313, 352, 366, 416
PleI GAGTC 2 cut(s) 209, 512
PpsI GAGTC 2 cut(s) 209, 512
PshBI ATTAAT 2 cut(s) 228, 332
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 1 cut(s) 395
RseI CAYNNNNRTG 1 cut(s) 111
SacII CCGCGG 1 cut(s) 415
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 2 cut(s) 228, 332
SatI GCNGC 5 cut(s) 88, 312, 351, 365, 415
Sau3AI GATC 1 cut(s) 187
SchI GAGTC 2 cut(s) 209, 512
ScrFI CCNGG 1 cut(s) 306
SetI ASST 7 cut(s) 49, 95, 132, 181, 316, 436, 454
SfaNI GCATC 3 cut(s) 198, 302, 435
Sfr303I CCGCGG 1 cut(s) 415
SgrBI CCGCGG 1 cut(s) 415
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
SphI GCATGC 1 cut(s) 277
SsiI CCGC 4 cut(s) 350, 365, 412, 414
StyD4I CCNGG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 346, 484
TaqI TCGA 5 cut(s) 138, 144, 376, 507, 516
TauI GCSGC 3 cut(s) 353, 367, 417
TfiI GAWTC 2 cut(s) 73, 337
Tru1I TTAA 2 cut(s) 228, 332
Tru9I TTAA 2 cut(s) 228, 332
TscAI CASTG 2 cut(s) 360, 387
TseI GCWGC 2 cut(s) 87, 311
TspDTI ATGAA 3 cut(s) 75, 95, 350
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 2 cut(s) 360, 387
VspI ATTAAT 2 cut(s) 228, 332
XceI RCATGY 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.