MD16G1013000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013160
Physical Location & Seq
Forward (+)
999362 .. 1000839
1478 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1013000.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGTGGGAATCCTCATTCCACTGTGCAAATCATCCCACACCGCCACCTCTCTTTCTACTCCTACTTTTCATACCTTCAGCTTTCTCTTCGTTTCTTCTTCATTTCTTCTCCTCTCTCTCGCTCTCTCTCTCTCTCTCTCTCTCTCTCTAAGATCTTCTCAGCTTCTGGCATGCAACAACACTACTATGAACAACATCTCTATGGGGTTTTCATGTGGGGATGATTTTGACGGAGAGGAATGCAAAGCTCCACTTTTATACGAAACGAGGGAGAACCAGCACAGCGGCAGCGGGTGCATGGATGTTCGCAATCATCATCGTCATCATATGCAGGTTGAAGTCGACTTCGAGTTTTGGCCGGTAGAGCATCCTCTCGAGCCACCGGATGAAGATCGCCCTGTCAAATGCCCAATGCCCGACTCTTCCGTCATTAATGAAGGAGGAAGGAAGGAGAAAAGGTTCTCAGAGAGCAATAGCATGCGTAAAAGAACAGAAGCATCATCTGCGGCTGCAGCAGCTTATAGCAATCAAGGGTTAATCGATCAGACGGTGGTGGCAGTGGCAACGCCGCCTCCTGCTCGACCGGTGCGTAAGAGGCACCACCACACTCTCACCCGCGGCGACTGTATGATATCACCTCTACGGAGAATGCCACCTCTTCCTTCTCTCCCAACGCAGAGCATTACTGTCTTTCAAATGCTCCAGCAGTTCGACAAGTTCGACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

26.95

Weight (kDa)

6.59

Isoelectric Point (pI)

60.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01913
fragaria_vesca FvH4_4g26140 FvH4_4g26140
malus_domestica MD13G1015500.v1.1 MD16G1013000.v1.1
prunus_persica Prupe.1G338300_v2.0.a1 Prupe.1G338300_v2.0.a1
pyrus_communis pycom13g01310 pycom16g01220
rosa_laevigata RLG00000006736
rosa_multiflora Rmu_sc0000312.1_g000026
rosa_roxburghii Rroxscaffold_5G00375150
rosa_rugosa Rorug04G0271100.1
rosa_samantha Rh4AG325400 Rh4BG333200 Rh4CG348400 Rh4DG329800
rosa_wichuraiana Rw4G028180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
Acc36I ACCTGC 1 cut(s) 322
AccB1I GGYRCC 1 cut(s) 597
AccI GTMKAC 1 cut(s) 342
AccII CGCG 1 cut(s) 618
AciI CCGC 7 cut(s) 42, 285, 291, 506, 569, 616, 618
AcoI YGGCCR 1 cut(s) 356
AcuI CTGAAG 1 cut(s) 61
AgeI ACCGGT 1 cut(s) 583
AgsI TTSAA 2 cut(s) 338, 695
AloI GAACNNNNNNTCC 2 cut(s) 443, 475
AluBI AGCT 4 cut(s) 81, 163, 248, 518
AluI AGCT 4 cut(s) 81, 163, 248, 518
AlwNI CAGNNNCTG 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 1 cut(s) 356
ApeKI GCWGC 4 cut(s) 288, 509, 512, 515
AseI ATTAAT 1 cut(s) 432
AsiGI ACCGGT 1 cut(s) 583
Asp700I GAANNNNTTC 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 604, 627
AvaI CYCGRG 1 cut(s) 374
BanI GGYRCC 1 cut(s) 597
BbvI GCAGC 4 cut(s) 300, 496, 524, 527
BfmI CTRYAG 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 322
BglII AGATCT 1 cut(s) 152
BisI GCNGC 8 cut(s) 286, 289, 507, 510, 513, 516, 569, 619
BlsI GCNGC 8 cut(s) 287, 290, 508, 511, 514, 517, 570, 620
BmeT110I CYCGRG 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 599
BmsI GCATC 2 cut(s) 376, 506
BpmI CTGGAG 1 cut(s) 686
Bsa29I ATCGAT 1 cut(s) 540
BsaBI GATNNNNATC 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 616
BsaWI WCCGGW 2 cut(s) 382, 583
BsaXI ACNNNNNCTCC 4 cut(s) 443, 473, 556, 586
Bse118I RCCGGY 2 cut(s) 358, 583
Bse8I GATNNNNATC 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 616
BseGI GGATG 5 cut(s) 32, 226, 307, 367, 391
BseJI GATNNNNATC 1 cut(s) 390
BseMII CTCAG 2 cut(s) 173, 477
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 4 cut(s) 300, 496, 524, 527
Bsh1236I CGCG 1 cut(s) 618
Bsh1285I CGRYCG 1 cut(s) 584
BshFI GGCC 1 cut(s) 358
BshNI GGYRCC 1 cut(s) 597
BshTI ACCGGT 1 cut(s) 583
BshVI ATCGAT 1 cut(s) 540
BsiEI CGRYCG 1 cut(s) 584
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 3 cut(s) 359, 383, 584
BsmI GAATGC 2 cut(s) 245, 654
BsnI GGCC 1 cut(s) 358
BsoBI CYCGRG 1 cut(s) 374
Bsp143I GATC 3 cut(s) 152, 391, 541
BspACI CCGC 7 cut(s) 42, 285, 291, 506, 569, 616, 618
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 2 cut(s) 172, 476
BspDI ATCGAT 1 cut(s) 540
BspFNI CGCG 1 cut(s) 618
BspLI GGNNCC 1 cut(s) 599
BspMAI CTGCAG 1 cut(s) 514
BspMI ACCTGC 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 597
BsrFI RCCGGY 2 cut(s) 358, 583
BssAI RCCGGY 2 cut(s) 358, 583
BssECI CCNNGG 1 cut(s) 616
BssMI GATC 3 cut(s) 152, 391, 541
Bst4CI ACNGT 4 cut(s) 24, 550, 626, 688
Bst6I CTCTTC 3 cut(s) 92, 427, 663
BstAPI GCANNNNNTGC 2 cut(s) 294, 503
BstC8I GCNNGC 2 cut(s) 172, 479
BstDEI CTNAG 3 cut(s) 149, 159, 463
BstDSI CCRYGG 1 cut(s) 616
BstF5I GGATG 5 cut(s) 32, 226, 307, 367, 391
BstFNI CGCG 1 cut(s) 618
BstKTI GATC 3 cut(s) 155, 394, 544
BstMBI GATC 3 cut(s) 152, 391, 541
BstMCI CGRYCG 1 cut(s) 584
BstMWI GCNNNNNNNGC 6 cut(s) 294, 364, 503, 512, 515, 595
BstNSI RCATGY 2 cut(s) 174, 481
BstSFI CTRYAG 1 cut(s) 510
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 4 cut(s) 300, 496, 524, 527
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
Bsu15I ATCGAT 1 cut(s) 540
BsuRI GGCC 1 cut(s) 358
BsuTUI ATCGAT 1 cut(s) 540
BtgI CCRYGG 1 cut(s) 616
BtsCI GGATG 5 cut(s) 32, 226, 307, 367, 391
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 2 cut(s) 20, 564
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 2 cut(s) 172, 479
CaiI CAGNNNCTG 1 cut(s) 166
Cfr10I RCCGGY 2 cut(s) 358, 583
Cfr42I CCGCGG 1 cut(s) 619
ClaI ATCGAT 1 cut(s) 540
CspAI ACCGGT 1 cut(s) 583
CviAII CATG 4 cut(s) 171, 213, 298, 478
CviJI RGCY 7 cut(s) 81, 163, 248, 358, 379, 509, 518
CviKI_1 RGCY 7 cut(s) 81, 163, 248, 358, 379, 509, 518
DdeI CTNAG 3 cut(s) 149, 159, 463
DpnI GATC 3 cut(s) 154, 393, 543
DpnII GATC 3 cut(s) 152, 391, 541
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 356
Eam1104I CTCTTC 3 cut(s) 92, 427, 663
EarI CTCTTC 3 cut(s) 92, 427, 663
Eco32I GATATC 1 cut(s) 633
Eco57I CTGAAG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 374
EcoRV GATATC 1 cut(s) 633
FaeI CATG 4 cut(s) 174, 216, 301, 481
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FatI CATG 4 cut(s) 170, 212, 297, 477
FauI CCCGC 2 cut(s) 284, 623
FauNDI CATATG 1 cut(s) 327
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 8 cut(s) 286, 289, 507, 510, 513, 516, 569, 619
FokI GGATG 5 cut(s) 19, 233, 314, 354, 398
Fsp4HI GCNGC 8 cut(s) 286, 289, 507, 510, 513, 516, 569, 619
GluI GCNGC 8 cut(s) 286, 289, 507, 510, 513, 516, 569, 619
GsuI CTGGAG 1 cut(s) 686
HaeIII GGCC 1 cut(s) 358
HapII CCGG 3 cut(s) 359, 383, 584
Hin1II CATG 4 cut(s) 174, 216, 301, 481
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 3 cut(s) 9, 419, 722
HpaII CCGG 3 cut(s) 359, 383, 584
HphI GGTGA 2 cut(s) 604, 627
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 2 cut(s) 466, 546
Hpy188III TCNNGA 2 cut(s) 374, 726
Hpy8I GTNNAC 1 cut(s) 343
HpyAV CCTTC 5 cut(s) 85, 431, 438, 442, 672
HpyCH4III ACNGT 4 cut(s) 24, 550, 626, 688
HpyCH4V TGCA 6 cut(s) 27, 174, 243, 297, 331, 512
HpyF10VI GCNNNNNNNGC 6 cut(s) 294, 364, 503, 512, 515, 595
HpyF3I CTNAG 3 cut(s) 149, 159, 463
Hsp92II CATG 4 cut(s) 174, 216, 301, 481
KspI CCGCGG 1 cut(s) 619
Kzo9I GATC 3 cut(s) 152, 391, 541
LmnI GCTCC 2 cut(s) 253, 705
LpnPI CCDG 9 cut(s) 152, 290, 317, 372, 396, 411, 588, 597, 716
Lsp1109I GCAGC 4 cut(s) 300, 496, 524, 527
LweI GCATC 2 cut(s) 376, 506
MalI GATC 3 cut(s) 154, 393, 543
MboI GATC 3 cut(s) 152, 391, 541
MboII GAAGA 8 cut(s) 79, 87, 90, 98, 147, 401, 414, 650
MflI RGATCY 1 cut(s) 152
MlyI GAGTC 2 cut(s) 413, 716
MroXI GAANNNNTTC 1 cut(s) 458
MseI TTAA 2 cut(s) 432, 536
MslI CAYNNNNRTG 2 cut(s) 185, 200
MspA1I CMGCKG 3 cut(s) 285, 291, 618
MspI CCGG 3 cut(s) 359, 383, 584
Mva1269I GAATGC 2 cut(s) 245, 654
MvnI CGCG 1 cut(s) 618
MwoI GCNNNNNNNGC 6 cut(s) 294, 364, 503, 512, 515, 595
NdeI CATATG 1 cut(s) 327
NdeII GATC 3 cut(s) 152, 391, 541
NlaIII CATG 4 cut(s) 174, 216, 301, 481
NlaIV GGNNCC 1 cut(s) 599
NspI RCATGY 2 cut(s) 174, 481
PaeI GCATGC 2 cut(s) 174, 481
PaeR7I CTCGAG 1 cut(s) 374
PcsI WCGNNNNNNNCGW 2 cut(s) 586, 717
PctI GAATGC 2 cut(s) 245, 654
PdmI GAANNNNTTC 1 cut(s) 458
PfeI GAWTC 1 cut(s) 9
PinAI ACCGGT 1 cut(s) 583
PkrI GCNGC 8 cut(s) 287, 290, 508, 511, 514, 517, 570, 620
PleI GAGTC 2 cut(s) 413, 716
PpsI GAGTC 2 cut(s) 413, 716
PshBI ATTAAT 1 cut(s) 432
PspN4I GGNNCC 1 cut(s) 599
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 152
RseI CAYNNNNRTG 2 cut(s) 185, 200
SacII CCGCGG 1 cut(s) 619
SalI GTCGAC 1 cut(s) 341
SaqAI TTAA 2 cut(s) 432, 536
SatI GCNGC 8 cut(s) 286, 289, 507, 510, 513, 516, 569, 619
Sau3AI GATC 3 cut(s) 152, 391, 541
SchI GAGTC 2 cut(s) 413, 716
SfaNI GCATC 2 cut(s) 376, 506
SfcI CTRYAG 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 374
Sfr303I CCGCGG 1 cut(s) 619
SgrBI CCGCGG 1 cut(s) 619
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 185, 200
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SphI GCATGC 2 cut(s) 174, 481
SsiI CCGC 7 cut(s) 42, 285, 291, 506, 569, 616, 618
TaaI ACNGT 4 cut(s) 24, 550, 626, 688
TaqI TCGA 7 cut(s) 342, 348, 375, 540, 580, 711, 720
TauI GCSGC 4 cut(s) 288, 509, 571, 621
TfiI GAWTC 1 cut(s) 9
Tru1I TTAA 2 cut(s) 432, 536
Tru9I TTAA 2 cut(s) 432, 536
TscAI CASTG 2 cut(s) 27, 564
TseI GCWGC 4 cut(s) 288, 509, 512, 515
TspDTI ATGAA 6 cut(s) 59, 90, 201, 203, 402, 450
TspGWI ACGGA 3 cut(s) 246, 415, 658
TspRI CASTG 2 cut(s) 27, 564
VspI ATTAAT 1 cut(s) 432
XceI RCATGY 2 cut(s) 174, 481
XhoI CTCGAG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.