Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011751

Physical Location & Seq

Genomic Coordinates Forward (+)

3542062 .. 3548264

Gene Structure 6203 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1049800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2397 bp

ATGGTGAAGTTCTCCAAGCAATTCGAAGGGCAGCTCGTCCCTGAATGGAAGGATGCATTCGTCGATTACTGGCAGCTCAAGAAGGACCTCAAAAAAATCAATCTCCTTGACTCTAACAACAACAAAAACTCACCTACTAATCACCAAAATAGCTCGTCTTTATCCAACACTCTCGTCACTTCCATTAGGAAGTTCACTCCTTTTGGCCATCCACATAGAGAACATGACCTCATACATGTTCACAGGAAGCTTGCCTCATCAGCAAGCAAGGGAGACATGTATGAGACTGAATTGTTAGAGCAGTTTGCTGATACTAATGCTGCGAAAGAGTTTTTCTCATTCCTCGACCTTCAGCTCAACAAGGTGAATCAGTTTTTCAGAACAAAGGAGAAAGAGTTCATGGAGAGAGGGGAGTCCTTGAAGAAACAAATGGATATACTCATTGAGCTCAAAACCGCCTTCAAGAAACAGCGCGGCAAAGGAGCTTTTGCGCTGAATTCCAAGGAGGATATTTCCATGTCAGTCTCCTTCACATCTGATGAGGATTCTGTTAAGGATAAAACAGAGCAAGAACAGCTGCAAGACTGCACAGAAGACTTGGAGAAAAATGAAGTGGCAAACTCGGAAGGAACGGCATACTCGGAAGGCACGATATCCGGTGAATTGGGAAAATCAATTAGAACGAAAAGTGAAGATGGTGGAAAGCTAAGGAGCATGTCAAGTCGTTCTTTCAGCTTCCAAGGGAAGAACTTGAAGATAAACATTCCTCTAACAACCCCGTCGCGCACTTTCTCAGCGATCAGTTACATAGTTTGGGAAGATCTGGTGAATCAGTCCTCGAAAAAATGCGGCGCAGACAGTGGTAAGCTGCACATCAACAAAACTAAGTTGCATCATGCGGAAAAAATGATCCGAGGAGCTTTCGTCGAGCTCTACAAAGGGTTGGGATACCTCAAAACTTACAGGAACTTGAACATGCTTGCTTTTATAAAGATTTTAAAGAAGTTTGACAAAGTCACTGGAAAACAAGTTCTTCCGATTTATCTAAAAGTTGTGGAGAGTTCCTATTTCAACAGCTCAGATAAGGTGATGAACTTAGGAGATGAAGTTGAGGAGCTTTTCACCAAACACTTCGCTGAAGAAGACCGGCGAAAGGCCATAAAGTATCTTAAACCGACTGAGCGTAAAGAATCACATTCTGTAACATTTTTCATTGGTTTATTTACGGGATGTTTTATCGCACTCTTAACTGGATATGTCATTATGGCTCATATAACGGGATTGTACAAACGGCAGCCAAATTCAGTCTACATGGAAACTGCCTACCCTGTACTTAGCATGTTCAGCCTACTGTTTCTACACTTCTTCTTATACGGCTGCAACATTTTTGCGTGGAGAAAGGCGCGTATAAATTACAGTTTCATCTTTGAACTGTCCCAGACGAAGGAGCTAAAGTACAGAGATGTATTCTTGATCTGCACCATGTCGTTGACTACGGTGGTCGGAGTCATGTGTGTTCATTTGTTACTGCTAACAAAAGGCTACTCATATGCCCAAGTTCAAGCAATTCCTGGCCTTCTTTTGTTGGTGTTTTTACTACTACTTGTGTGCCCCTTCAACATCATTTACCGATCAACCCGTTTCCGCCTTCTTCGTGTCCTAAGAAATATCATTCTGTCGCCTCTGTACAAGGTTGTCATGCTGGACTTCTTCATGGCAGATCAACTTTGTAGTCAGGTTCCGATGCTTAGGAACCTCGAGTATGTGGCGTGTTACTACATAACCGGAAGCTACAAAACTCAGGACTATGGCTACTGCATGAGGGCGGGGCATTACCGAGATCTTGCCTATGCCGTGTCCTTCCTACCTTATTACTGGAGAGCAATGCAGTGTGCTCGGCGGTGGTTTGATGAGGGACACACAAGCCACCTTGTGAATCTAGGCAAATATGTGTCAGCAATGTTAGCAGCAGTAGCTAAAGTAGCTTATGAGAAAGAAAGGAGCATTGGATGGCTCTGTCTTGTCGTGATCATATCAACTTTCGCAACAATTTACCAATTGTATTGGGACTTTGTAAAGGATTGGGGTTTGCTACAAATGAATTCCAAGAATCCTTTGCTTAGGAACGAATTAATGATTCGCCGAAAGTTCATATACTACATCTCAATGGGATTGAACCTTATTCTCAGGCTAGCTTGGATGCAAACTGTTCTCCATTCAAGTTTTGGACATGTAGATTACAGGGTAACCGGGCTGTTTTTGGCAGCTCTTGAAGTCATCAGAAGAGGGTTGTGGAACTTTTTCAGGTTAGAGAATGAGCATCTGAATAATGCAGGCAAGTTTAGAGCAGTTAAGACAGTACCACTGCCTTTTCATGAAGTGGATGATCAGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0033554 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0071496 GO:0071944 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

799

Amino Acids

92.41

Weight (kDa)

9.27

Isoelectric Point (pI)

36.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	2 - 352	2.6e-66	SPX domain
EXS	PF03124	442 - 775	1.3e-98	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011751)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68740 AT1G68740
fragaria_vesca	FvH4_4g28410
malus_domestica	MD13G1049800.v1.1 MD16G1050800.v1.1
prunus_persica	Prupe.1G301900_v2.0.a1
pyrus_communis	pycom13g04410 pycom13g04420 pycom16g04450
rosa_chinensis	RchiOBHm_Chr4g0436361
rosa_laevigata	RLG00000006525
rosa_multiflora	Rmu_sc0006449.1_g000007
rosa_roxburghii	Rroxscaffold_5G00377530
rosa_rugosa	Rorug04G0293300
rosa_samantha	Rh4AG347500 Rh4BG356300 Rh4CG370900 Rh4DG350000
rosa_wichuraiana	Rw4G030380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	989
AccI	GTMKAC	1 cut(s)	1308
AccII	CGCG	3 cut(s)	474, 784, 1405
AciI	CCGC	7 cut(s)	456, 474, 849, 899, 1645, 1826, 1900
AclWI	GGATC	1 cut(s)	904
AcoI	YGGCCR	1 cut(s)	205
AcsI	RAATTY	3 cut(s)	496, 1300, 2101
AcuI	CTGAAG	2 cut(s)	335, 1158
AdeI	CACNNNGTG	1 cut(s)	1933
AfaI	GTAC	5 cut(s)	1286, 1332, 1457, 1688, 2361
AfiI	CCNNNNNNNGG	2 cut(s)	1153, 1444
AflIII	ACRYGT	3 cut(s)	235, 276, 2230
AjnI	CCWGG	1 cut(s)	1570
AjuI	GAANNNNNNNTTGG	2 cut(s)	1989, 2021
AloI	GAACNNNNNNTCC	4 cut(s)	380, 412, 1014, 1046
Alw21I	GWGCWC	3 cut(s)	450, 933, 1897
Alw26I	GTCTC	3 cut(s)	267, 278, 529
AlwI	GGATC	1 cut(s)	904
Ama87I	CYCGRG	1 cut(s)	1757
AoxI	GGCC	3 cut(s)	205, 1155, 1573
ApeKI	GCWGC	9 cut(s)	31, 73, 320, 577, 868, 1294, 1377, 1967, 2264
ApoI	RAATTY	3 cut(s)	496, 1300, 2101
AseI	ATTAAT	1 cut(s)	2132
Asp700I	GAANNNNTTC	2 cut(s)	395, 2300
AspLEI	GCGC	5 cut(s)	474, 493, 786, 854, 1405
AspS9I	GGNCC	1 cut(s)	85
AsuC2I	CCSGG	1 cut(s)	2251
AsuHPI	GGTGA	8 cut(s)	16, 123, 134, 376, 671, 838, 1099, 1114
AsuII	TTCGAA	1 cut(s)	24
AsuNHI	GCTAGC	1 cut(s)	2191
AvaI	CYCGRG	1 cut(s)	1757
AvaII	GGWCC	1 cut(s)	85
BaeGI	GKGCMC	1 cut(s)	1613
BalI	TGGCCA	1 cut(s)	207
BanII	GRGCYC	2 cut(s)	450, 933
BbsI	GAAGAC	2 cut(s)	600, 1149
Bbv12I	GWGCWC	3 cut(s)	450, 933, 1897
BbvI	GCAGC	9 cut(s)	43, 85, 307, 564, 855, 1306, 1364, 1979, 2276
BccI	CCATC	3 cut(s)	216, 689, 2004
BceAI	ACGGC	4 cut(s)	648, 1307, 1390, 1838
BcgI	CGANNNNNNTGC	2 cut(s)	1877, 1911
BciT130I	CCWGG	1 cut(s)	1572
BciVI	GTATCC	1 cut(s)	941
BclI	TGATCA	2 cut(s)	2028, 2386
BcnI	CCSGG	1 cut(s)	2251
BcoDI	GTCTC	3 cut(s)	267, 278, 529
BfaI	CTAG	2 cut(s)	1940, 2192
BfuI	GTATCC	1 cut(s)	941
BglII	AGATCT	2 cut(s)	820, 1840
Bme1390I	CCNGG	2 cut(s)	1572, 2251
Bme18I	GGWCC	1 cut(s)	85
BmeT110I	CYCGRG	1 cut(s)	1757
BmgT120I	GGNCC	1 cut(s)	85
BmiI	GGNNCC	2 cut(s)	1740, 1754
BmrFI	CCNGG	2 cut(s)	1572, 2251
BmsI	GCATC	5 cut(s)	43, 901, 1734, 2190, 2329
BmtI	GCTAGC	1 cut(s)	2195
BpiI	GAAGAC	2 cut(s)	600, 1149
BplI	GAGNNNNNCTC	2 cut(s)	320, 352
BpmI	CTGGAG	1 cut(s)	1897
Bpu10I	CCTNAGC	3 cut(s)	707, 1748, 2120
Bpu14I	TTCGAA	1 cut(s)	24
BpuEI	CTTGAG	1 cut(s)	62
BpuMI	CCSGG	1 cut(s)	2251
BsaJI	CCNNGG	3 cut(s)	501, 739, 913
BsaWI	WCCGGW	2 cut(s)	656, 1784
BsaXI	ACNNNNNCTCC	8 cut(s)	380, 410, 909, 939, 1092, 1122, 1439, 1469
Bsc4I	CCNNNNNNNGG	2 cut(s)	1153, 1444
Bse118I	RCCGGY	1 cut(s)	1146
Bse1I	ACTGG	4 cut(s)	74, 1024, 1255, 1880
Bse3DI	GCAATG	2 cut(s)	1890, 1965
BseBI	CCWGG	1 cut(s)	1572
BseDI	CCNNGG	3 cut(s)	501, 739, 913
BseGI	GGATG	6 cut(s)	58, 208, 1235, 2015, 2205, 2389
BseLI	CCNNNNNNNGG	2 cut(s)	1153, 1444
BseMI	GCAATG	2 cut(s)	1890, 1965
BseMII	CTCAG	5 cut(s)	807, 1092, 1170, 1814, 2200
BseNI	ACTGG	4 cut(s)	74, 1024, 1255, 1880
BseRI	GAGGAG	2 cut(s)	930, 1127
BseSI	GKGCMC	1 cut(s)	1613
BseXI	GCAGC	9 cut(s)	43, 85, 307, 564, 855, 1306, 1364, 1979, 2276
BsgI	GTGCAG	3 cut(s)	571, 854, 1462
Bsh1236I	CGCG	3 cut(s)	474, 784, 1405
BshFI	GGCC	3 cut(s)	207, 1157, 1575
BsiHKAI	GWGCWC	3 cut(s)	450, 933, 1897
BsiHKCI	CYCGRG	1 cut(s)	1757
BsiSI	CCGG	4 cut(s)	657, 1147, 1785, 2250
BslFI	GGGAC	4 cut(s)	23, 1420, 1929, 2081
BslI	CCNNNNNNNGG	2 cut(s)	1153, 1444
BsmAI	GTCTC	3 cut(s)	267, 278, 529
BsmFI	GGGAC	4 cut(s)	23, 1420, 1929, 2081
BsmI	GAATGC	1 cut(s)	56
BsnI	GGCC	3 cut(s)	207, 1157, 1575
BsoBI	CYCGRG	1 cut(s)	1757
Bsp119I	TTCGAA	1 cut(s)	24
Bsp1286I	GDGCHC	4 cut(s)	450, 933, 1613, 1897
Bsp1407I	TGTACA	2 cut(s)	1284, 1686
Bsp143I	GATC	9 cut(s)	798, 820, 909, 1473, 1631, 1720, 1840, 2028, 2386
BspACI	CCGC	7 cut(s)	456, 474, 849, 899, 1645, 1826, 1900
BspANI	GGCC	3 cut(s)	207, 1157, 1575
BspCNI	CTCAG	5 cut(s)	806, 1091, 1171, 1813, 2199
BspFNI	CGCG	3 cut(s)	474, 784, 1405
BspHI	TCATGA	1 cut(s)	2374
BspLI	GGNNCC	2 cut(s)	1740, 1754
BspOI	GCTAGC	1 cut(s)	2195
BspPI	GGATC	1 cut(s)	904
BspT104I	TTCGAA	1 cut(s)	24
BsrDI	GCAATG	2 cut(s)	1890, 1965
BsrFI	RCCGGY	1 cut(s)	1146
BsrGI	TGTACA	2 cut(s)	1284, 1686
BsrI	ACTGG	4 cut(s)	74, 1024, 1255, 1880
BssAI	RCCGGY	1 cut(s)	1146
BssECI	CCNNGG	3 cut(s)	501, 739, 913
BssMI	GATC	9 cut(s)	798, 820, 909, 1473, 1631, 1720, 1840, 2028, 2386
BssT1I	CCWWGG	2 cut(s)	501, 739
Bst2UI	CCWGG	1 cut(s)	1572
Bst4CI	ACNGT	7 cut(s)	860, 1353, 1418, 1434, 1498, 2209, 2359
Bst6I	CTCTTC	1 cut(s)	2278
BstAUI	TGTACA	2 cut(s)	1284, 1686
BstBI	TTCGAA	1 cut(s)	24
BstC8I	GCNNGC	5 cut(s)	252, 265, 981, 2193, 2335
BstEII	GGTNACC	1 cut(s)	2245
BstF5I	GGATG	6 cut(s)	58, 208, 1235, 2015, 2205, 2389
BstFNI	CGCG	3 cut(s)	474, 784, 1405
BstHHI	GCGC	5 cut(s)	474, 493, 786, 854, 1405
BstKTI	GATC	9 cut(s)	801, 823, 912, 1476, 1634, 1723, 1843, 2031, 2389
BstMAI	GTCTC	3 cut(s)	267, 278, 529
BstMBI	GATC	9 cut(s)	798, 820, 909, 1473, 1631, 1720, 1840, 2028, 2386
BstMWI	GCNNNNNNNGC	6 cut(s)	260, 574, 1344, 1964, 1973, 1982
BstNI	CCWGG	1 cut(s)	1572
BstNSI	RCATGY	6 cut(s)	239, 280, 718, 979, 1342, 2234
BstPI	GGTNACC	1 cut(s)	2245
BstSCI	CCNGG	2 cut(s)	1570, 2249
BstSLI	GKGCMC	1 cut(s)	1613
BstUI	CGCG	3 cut(s)	474, 784, 1405
BstV1I	GCAGC	9 cut(s)	43, 85, 307, 564, 855, 1306, 1364, 1979, 2276
BstV2I	GAAGAC	2 cut(s)	600, 1149
BstX2I	RGATCY	2 cut(s)	820, 1840
BstYI	RGATCY	2 cut(s)	820, 1840
BsuI	GTATCC	1 cut(s)	941
BsuRI	GGCC	3 cut(s)	207, 1157, 1575
BtsCI	GGATG	6 cut(s)	58, 208, 1235, 2015, 2205, 2389
BtsI	GCAGTG	2 cut(s)	1895, 2363
BtsIMutI	CAGTG	4 cut(s)	865, 1017, 1895, 2363
Cac8I	GCNNGC	5 cut(s)	252, 265, 981, 2193, 2335
CciI	TCATGA	1 cut(s)	2374
CfoI	GCGC	5 cut(s)	474, 493, 786, 854, 1405
Cfr10I	RCCGGY	1 cut(s)	1146
Cfr13I	GGNCC	1 cut(s)	85
Csp6I	GTAC	5 cut(s)	1285, 1331, 1456, 1687, 2360
CviQI	GTAC	5 cut(s)	1285, 1331, 1456, 1687, 2360
DpnI	GATC	9 cut(s)	800, 822, 911, 1475, 1633, 1722, 1842, 2030, 2388
DpnII	GATC	9 cut(s)	798, 820, 909, 1473, 1631, 1720, 1840, 2028, 2386
DraI	TTTAAA	1 cut(s)	999
DraIII	CACNNNGTG	1 cut(s)	1933
EaeI	YGGCCR	1 cut(s)	205
Eam1104I	CTCTTC	1 cut(s)	2278
EarI	CTCTTC	1 cut(s)	2278
EciI	GGCGGA	1 cut(s)	1634
Ecl136II	GAGCTC	2 cut(s)	448, 931
Eco130I	CCWWGG	2 cut(s)	501, 739
Eco24I	GRGCYC	2 cut(s)	450, 933
Eco32I	GATATC	1 cut(s)	654
Eco47I	GGWCC	1 cut(s)	85
Eco53kI	GAGCTC	2 cut(s)	448, 931
Eco57I	CTGAAG	2 cut(s)	335, 1158
Eco88I	CYCGRG	1 cut(s)	1757
Eco91I	GGTNACC	1 cut(s)	2245
EcoICRI	GAGCTC	2 cut(s)	448, 931
EcoO109I	RGGNCCY	1 cut(s)	85
EcoO65I	GGTNACC	1 cut(s)	2245
EcoRI	GAATTC	2 cut(s)	496, 2101
EcoRII	CCWGG	1 cut(s)	1570
EcoRV	GATATC	1 cut(s)	654
EcoT14I	CCWWGG	2 cut(s)	501, 739
EcoT22I	ATGCAT	1 cut(s)	58
EcoT38I	GRGCYC	2 cut(s)	450, 933
ErhI	CCWWGG	2 cut(s)	501, 739
FalI	AAGNNNNNCTT	2 cut(s)	712, 744
FaqI	GGGAC	4 cut(s)	23, 1420, 1929, 2081
FauI	CCCGC	1 cut(s)	1819
FauNDI	CATATG	1 cut(s)	1549
FbaI	TGATCA	2 cut(s)	2028, 2386
FblI	GTMKAC	1 cut(s)	1308
FokI	GGATG	5 cut(s)	65, 195, 1242, 2022, 2212
FriOI	GRGCYC	2 cut(s)	450, 933
FspBI	CTAG	2 cut(s)	1940, 2192
GlaI	GCGC	5 cut(s)	473, 492, 785, 853, 1404
GsuI	CTGGAG	1 cut(s)	1897
HaeIII	GGCC	3 cut(s)	207, 1157, 1575
HapII	CCGG	4 cut(s)	657, 1147, 1785, 2250
HhaI	GCGC	5 cut(s)	474, 493, 786, 854, 1405
Hin6I	GCGC	5 cut(s)	472, 491, 784, 852, 1403
HinP1I	GCGC	5 cut(s)	472, 491, 784, 852, 1403
HincII	GTYRAC	1 cut(s)	1491
HindII	GTYRAC	1 cut(s)	1491
HindIII	AAGCTT	1 cut(s)	248
HpaII	CCGG	4 cut(s)	657, 1147, 1785, 2250
HphI	GGTGA	8 cut(s)	16, 123, 134, 376, 671, 838, 1099, 1114
Hpy166II	GTNNAC	4 cut(s)	195, 241, 1309, 1491
Hpy188III	TCNNGA	8 cut(s)	79, 463, 1471, 1802, 2026, 2270, 2375, 2390
Hpy8I	GTNNAC	4 cut(s)	195, 241, 1309, 1491
Hpy99I	CGWCG	3 cut(s)	65, 784, 929
HpyCH4III	ACNGT	7 cut(s)	860, 1353, 1418, 1434, 1498, 2209, 2359
HpyF10VI	GCNNNNNNNGC	6 cut(s)	260, 574, 1344, 1964, 1973, 1982
HspAI	GCGC	5 cut(s)	472, 491, 784, 852, 1403
Ksp22I	TGATCA	2 cut(s)	2028, 2386
Kzo9I	GATC	9 cut(s)	798, 820, 909, 1473, 1631, 1720, 1840, 2028, 2386
LmnI	GCTCC	6 cut(s)	482, 711, 917, 1114, 1447, 2001
Lsp1109I	GCAGC	9 cut(s)	43, 85, 307, 564, 855, 1306, 1364, 1979, 2276
LweI	GCATC	5 cut(s)	43, 901, 1734, 2190, 2329
MaeI	CTAG	2 cut(s)	1940, 2192
MaeIII	GTNAC	7 cut(s)	175, 803, 1015, 1201, 1524, 1772, 2245
MalI	GATC	9 cut(s)	800, 822, 911, 1475, 1633, 1722, 1842, 2030, 2388
MboI	GATC	9 cut(s)	798, 820, 909, 1473, 1631, 1720, 1840, 2028, 2386
MfeI	CAATTG	1 cut(s)	2057
MflI	RGATCY	2 cut(s)	820, 1840
MhlI	GDGCHC	4 cut(s)	450, 933, 1613, 1897
MlsI	TGGCCA	1 cut(s)	207
MluNI	TGGCCA	1 cut(s)	207
MlyI	GAGTC	3 cut(s)	104, 422, 1515
MmeI	TCCRAC	2 cut(s)	189, 1483
Mox20I	TGGCCA	1 cut(s)	207
Mph1103I	ATGCAT	1 cut(s)	58
MroXI	GAANNNNTTC	2 cut(s)	395, 2300
MscI	TGGCCA	1 cut(s)	207
MseI	TTAA	6 cut(s)	552, 998, 1170, 1247, 2132, 2352
MslI	CAYNNNNRTG	1 cut(s)	2165
Msp20I	TGGCCA	1 cut(s)	207
MspA1I	CMGCKG	1 cut(s)	577
MspI	CCGG	4 cut(s)	657, 1147, 1785, 2250
MspR9I	CCNGG	2 cut(s)	1572, 2251
MunI	CAATTG	1 cut(s)	2057
Mva1269I	GAATGC	1 cut(s)	56
MvaI	CCWGG	1 cut(s)	1572
MvnI	CGCG	3 cut(s)	474, 784, 1405
MwoI	GCNNNNNNNGC	6 cut(s)	260, 574, 1344, 1964, 1973, 1982
NciI	CCSGG	1 cut(s)	2251
NdeI	CATATG	1 cut(s)	1549
NdeII	GATC	9 cut(s)	798, 820, 909, 1473, 1631, 1720, 1840, 2028, 2386
NheI	GCTAGC	1 cut(s)	2191
NlaIV	GGNNCC	2 cut(s)	1740, 1754
NmeAIII	GCCGAG	1 cut(s)	1876
NmuCI	GTSAC	2 cut(s)	175, 1015
NsiI	ATGCAT	1 cut(s)	58
NspI	RCATGY	6 cut(s)	239, 280, 718, 979, 1342, 2234
NspV	TTCGAA	1 cut(s)	24
PaeR7I	CTCGAG	1 cut(s)	1757
PagI	TCATGA	1 cut(s)	2374
PciI	ACATGT	3 cut(s)	235, 276, 2230
PcsI	WCGNNNNNNNCGW	1 cut(s)	647
PctI	GAATGC	1 cut(s)	56
PdmI	GAANNNNTTC	2 cut(s)	395, 2300
PfeI	GAWTC	7 cut(s)	367, 545, 829, 1190, 1936, 2110, 2137
PflFI	GACNNNGTC	1 cut(s)	1013
PleI	GAGTC	3 cut(s)	104, 421, 1514
PpsI	GAGTC	3 cut(s)	104, 421, 1514
PpuMI	RGGWCCY	1 cut(s)	85
PscI	ACATGT	3 cut(s)	235, 276, 2230
PshBI	ATTAAT	1 cut(s)	2132
PsiI	TTATAA	1 cut(s)	989
Psp124BI	GAGCTC	2 cut(s)	450, 933
Psp5II	RGGWCCY	1 cut(s)	85
Psp6I	CCWGG	1 cut(s)	1570
PspEI	GGTNACC	1 cut(s)	2245
PspGI	CCWGG	1 cut(s)	1570
PspN4I	GGNNCC	2 cut(s)	1740, 1754
PspPI	GGNCC	1 cut(s)	85
PspPPI	RGGWCCY	1 cut(s)	85
PspXI	VCTCGAGB	1 cut(s)	1757
PsuI	RGATCY	2 cut(s)	820, 1840
PsyI	GACNNNGTC	1 cut(s)	1013
PvuII	CAGCTG	1 cut(s)	577
RsaI	GTAC	5 cut(s)	1286, 1332, 1457, 1688, 2361
RsaNI	GTAC	5 cut(s)	1285, 1331, 1456, 1687, 2360
RseI	CAYNNNNRTG	1 cut(s)	2165
SacI	GAGCTC	2 cut(s)	450, 933
SaqAI	TTAA	6 cut(s)	552, 998, 1170, 1247, 2132, 2352
Sau3AI	GATC	9 cut(s)	798, 820, 909, 1473, 1631, 1720, 1840, 2028, 2386
Sau96I	GGNCC	1 cut(s)	85
SchI	GAGTC	3 cut(s)	104, 422, 1515
ScrFI	CCNGG	2 cut(s)	1572, 2251
SduI	GDGCHC	4 cut(s)	450, 933, 1613, 1897
SfaNI	GCATC	5 cut(s)	43, 901, 1734, 2190, 2329
Sfr274I	CTCGAG	1 cut(s)	1757
SfuI	TTCGAA	1 cut(s)	24
SinI	GGWCC	1 cut(s)	85
SlaI	CTCGAG	1 cut(s)	1757
SmiMI	CAYNNNNRTG	1 cut(s)	2165
SmlI	CTYRAG	2 cut(s)	77, 1757
SmoI	CTYRAG	2 cut(s)	77, 1757
SsiI	CCGC	7 cut(s)	456, 474, 849, 899, 1645, 1826, 1900
SspMI	CTAG	2 cut(s)	1940, 2192
SstI	GAGCTC	2 cut(s)	450, 933
StyD4I	CCNGG	2 cut(s)	1570, 2249
StyI	CCWWGG	2 cut(s)	501, 739
TaaI	ACNGT	7 cut(s)	860, 1353, 1418, 1434, 1498, 2209, 2359
TaqI	TCGA	6 cut(s)	24, 63, 345, 839, 927, 1758
TatI	WGTACW	4 cut(s)	1284, 1330, 1455, 1686
TauI	GCSGC	2 cut(s)	477, 852
TfiI	GAWTC	7 cut(s)	367, 545, 829, 1190, 1936, 2110, 2137
Tru1I	TTAA	6 cut(s)	552, 998, 1170, 1247, 2132, 2352
Tru9I	TTAA	6 cut(s)	552, 998, 1170, 1247, 2132, 2352
TscAI	CASTG	4 cut(s)	865, 1024, 1895, 2370
TseFI	GTSAC	2 cut(s)	175, 1015
TseI	GCWGC	9 cut(s)	31, 73, 320, 577, 868, 1294, 1377, 1967, 2264
Tsp45I	GTSAC	2 cut(s)	175, 1015
TspRI	CASTG	4 cut(s)	865, 1024, 1895, 2370
Tth111I	GACNNNGTC	1 cut(s)	1013
VpaK11BI	GGWCC	1 cut(s)	85
VspI	ATTAAT	1 cut(s)	2132
XapI	RAATTY	3 cut(s)	496, 1300, 2101
XceI	RCATGY	6 cut(s)	239, 280, 718, 979, 1342, 2234
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2222
XhoI	CTCGAG	1 cut(s)	1757
XmiI	GTMKAC	1 cut(s)	1308
XmnI	GAANNNNTTC	2 cut(s)	395, 2300
XspI	CTAG	2 cut(s)	1940, 2192
Zsp2I	ATGCAT	1 cut(s)	58

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.