Rroxscaffold_5G00377530

phosphate transporter PHO1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011751
Physical Location & Seq
Forward (+)
58137064 .. 58140207
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00377530.1

Sequence Viewer

Length: 1236 bp
ATGAGCACAAATTGGCTGGGAAGTCATAGCATCTCCGGCGGCGGTTTAGTAAACTTTCCGGCGAAAAGGTGGTCACTATACCTCTCCAAGTGGAGGAGGAATAGGATGGTTCACAAGAAGCTTGCCTCATCAGCAAGCAAGGGAGACATGTACGAAACTGAATTGTTCGAGCAACTTGCTGATACTGATGCTGCGAAAGAGTTTTTTGCATGCTTGGACCTTCAACTCAACAAGGTGAATCAGTTCTACCAGAAAAAGGAGAAAGAGTTCATGGAGAGAGGGCAGTCCTTGACGAAGCAGATGGATATACTCATTGAGCTCAAAACCGCTTTCAAGCAACAGCGCGCCAAAGGTGGAGCATCTGCTCAGGACTCCAAGGAGGAAGCCTCTATCTCATGCACCTTCTCATCTGAGGAGGACTCTGTGAAGGACAAAACGGAACTTGAACAGCTGCAAGAAACAGATGAGTTGGAGAAAAATGATGAAGTAGCCTACACAGAAGTCCCAATATCAGGCGAATTGGTGAAACCAATGAGAATGAAAAGTGAGGATATTGGGAAACAACTGAGGAGCGTCTCAAGCCGTTCTTTCAGTTGCCAAGGGAAGAACTTAAAGATAAACATTCCTCTGACTACACCGTCACGCACTTTCTCAGCAATCAGTTATTTAGTTTGGGAAGATCTAGTTAATCAGTCCTCAAAGAAATGTACTTCAGAAGGCATGAGTAAGCTGCACATCAACAAAACCAAGTTGCATCATGCAGATAAGATGATCAGAGGAGCTTTTGTTGAGCTGTACAAAGGCTTGGGATATCTCAAAACCTACAGGAACTTGAACATGCTTGCTTTTATAAAGATTTTAAAGAAGTTTGACAAGGCGACTGAAAAACAAGTGCTTCCAATCTATCTAAAAGTTGTTGAGAGTTCCTATTTCAACAGCTCAGATAAGGTTATGAATCTAGCAGATGAAGTTGAGGAGCTGTTTACCAAACACTTCGCTGAAGAAGACCGAAGAAAGGCCATGAAATACCTTAAACCACATCATCGCAAAGAATCACACTCTGTAACGTTCTTCATTGGTCTATTCACTGGGTGTTTCATTGCACTCTTTGCTGGATATGTCATTATGGCTCATATTACTGGGTTGTACAGAAGGCAACCAAATTCTATATACATGGAAACTGCCTACCCTATCCTCAGGCAAGTATTTCTGCAATTAAATTCTTTGGCATCTTAA

Protein Analysis

411

Amino Acids

47.16

Weight (kDa)

9.14

Isoelectric Point (pI)

54.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 38 - 306 9.5e-48 SPX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011751)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68740 AT1G68740
fragaria_vesca FvH4_4g28410
malus_domestica MD13G1049800.v1.1 MD16G1050800.v1.1
prunus_persica Prupe.1G301900_v2.0.a1
pyrus_communis pycom13g04410 pycom13g04420 pycom16g04450
rosa_chinensis RchiOBHm_Chr4g0436361
rosa_laevigata RLG00000006525
rosa_multiflora Rmu_sc0006449.1_g000007
rosa_roxburghii Rroxscaffold_5G00377530
rosa_rugosa Rorug04G0293300
rosa_samantha Rh4AG347500 Rh4BG356300 Rh4CG370900 Rh4DG350000
rosa_wichuraiana Rw4G030380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 851
AasI GACNNNNNNGTC 1 cut(s) 637
AccII CGCG 1 cut(s) 345
AciI CCGC 3 cut(s) 39, 42, 327
AclI AACGTT 1 cut(s) 1067
AcsI RAATTY 2 cut(s) 1162, 1219
AcuI CTGAAG 2 cut(s) 696, 1020
AdeI CACNNNGTG 1 cut(s) 1092
AfaI GTAC 4 cut(s) 152, 709, 797, 1148
AfiI CCNNNNNNNGG 4 cut(s) 93, 256, 512, 1015
AflIII ACRYGT 1 cut(s) 147
AgsI TTSAA 5 cut(s) 224, 334, 446, 835, 934
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 8 cut(s) 121, 319, 451, 730, 782, 793, 939, 979
AluI AGCT 8 cut(s) 121, 319, 451, 730, 782, 793, 939, 979
Alw21I GWGCWC 2 cut(s) 8, 321
Alw26I GTCTC 2 cut(s) 138, 580
AoxI GGCC 1 cut(s) 1017
ApeKI GCWGC 3 cut(s) 191, 451, 730
ApoI RAATTY 2 cut(s) 1162, 1219
ArsI GACNNNNNNTTYG 2 cut(s) 56, 88
Asp700I GAANNNNTTC 2 cut(s) 242, 266
AspLEI GCGC 2 cut(s) 345, 347
AspS9I GGNCC 1 cut(s) 217
AsuHPI GGTGA 2 cut(s) 247, 535
AvaII GGWCC 1 cut(s) 217
AxyI CCTNAGG 1 cut(s) 1196
BanII GRGCYC 1 cut(s) 321
BbsI GAAGAC 1 cut(s) 1011
Bbv12I GWGCWC 2 cut(s) 8, 321
BbvI GCAGC 3 cut(s) 178, 438, 717
BccI CCATC 2 cut(s) 100, 295
BceAI ACGGC 1 cut(s) 567
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BclI TGATCA 1 cut(s) 771
BcoDI GTCTC 2 cut(s) 138, 580
BfaI CTAG 2 cut(s) 683, 959
BfmI CTRYAG 1 cut(s) 823
BglII AGATCT 1 cut(s) 679
BisI GCNGC 4 cut(s) 40, 192, 452, 731
BlsI GCNGC 4 cut(s) 41, 193, 453, 732
Bme18I GGWCC 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 217
BmrI ACTGGG 2 cut(s) 1098, 1149
BmsI GCATC 4 cut(s) 39, 178, 368, 763
BmuI ACTGGG 2 cut(s) 1098, 1149
BpiI GAAGAC 1 cut(s) 1011
BplI GAGNNNNNCTC 4 cut(s) 371, 403, 404, 436
Bpu10I CCTNAGC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 562
BsaJI CCNNGG 2 cut(s) 375, 598
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 771, 801
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 256, 512, 1015
Bse1I ACTGG 2 cut(s) 1093, 1144
Bse21I CCTNAGG 1 cut(s) 1196
Bse3DI GCAATG 1 cut(s) 1098
BseDI CCNNGG 2 cut(s) 375, 598
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 4 cut(s) 93, 256, 512, 1015
BseMI GCAATG 1 cut(s) 1098
BseMII CTCAG 6 cut(s) 380, 402, 557, 666, 954, 1210
BseNI ACTGG 2 cut(s) 1093, 1144
BsePI GCGCGC 1 cut(s) 343
BseRI GAGGAG 5 cut(s) 109, 428, 583, 792, 989
BseXI GCAGC 3 cut(s) 178, 438, 717
BseYI CCCAGC 1 cut(s) 16
BsgI GTGCAG 1 cut(s) 716
Bsh1236I CGCG 1 cut(s) 345
BshFI GGCC 1 cut(s) 1019
BsiHKAI GWGCWC 2 cut(s) 8, 321
BsiSI CCGG 2 cut(s) 36, 59
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 4 cut(s) 93, 256, 512, 1015
BsmAI GTCTC 2 cut(s) 138, 580
BsmBI CGTCTC 1 cut(s) 580
BsmFI GGGAC 1 cut(s) 488
BsnI GGCC 1 cut(s) 1019
Bsp1286I GDGCHC 2 cut(s) 8, 321
Bsp1407I TGTACA 2 cut(s) 795, 1146
Bsp143I GATC 2 cut(s) 679, 771
BspACI CCGC 3 cut(s) 39, 42, 327
BspANI GGCC 1 cut(s) 1019
BspCNI CTCAG 6 cut(s) 379, 403, 558, 665, 953, 1209
BspFNI CGCG 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 1098
BsrGI TGTACA 2 cut(s) 795, 1146
BsrI ACTGG 2 cut(s) 1093, 1144
BssECI CCNNGG 2 cut(s) 375, 598
BssHII GCGCGC 1 cut(s) 343
BssMI GATC 2 cut(s) 679, 771
BssT1I CCWWGG 2 cut(s) 375, 598
Bst4CI ACNGT 1 cut(s) 639
BstAPI GCANNNNNTGC 1 cut(s) 1109
BstAUI TGTACA 2 cut(s) 795, 1146
BstC8I GCNNGC 5 cut(s) 123, 136, 211, 345, 843
BstDEI CTNAG 6 cut(s) 366, 411, 566, 652, 940, 1196
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 345
BstHHI GCGC 2 cut(s) 345, 347
BstKTI GATC 2 cut(s) 682, 774
BstMAI GTCTC 2 cut(s) 138, 580
BstMBI GATC 2 cut(s) 679, 771
BstMWI GCNNNNNNNGC 4 cut(s) 36, 131, 579, 1109
BstNSI RCATGY 3 cut(s) 151, 213, 841
BstSFI CTRYAG 1 cut(s) 823
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 3 cut(s) 178, 438, 717
BstV2I GAAGAC 1 cut(s) 1011
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
Bsu36I CCTNAGG 1 cut(s) 1196
BsuRI GGCC 1 cut(s) 1019
BtgZI GCGATG 1 cut(s) 1028
BtsCI GGATG 1 cut(s) 111
BtsIMutI CAGTG 1 cut(s) 1086
Cac8I GCNNGC 5 cut(s) 123, 136, 211, 345, 843
CfoI GCGC 2 cut(s) 345, 347
Cfr13I GGNCC 1 cut(s) 217
CseI GACGC 1 cut(s) 562
Csp6I GTAC 4 cut(s) 151, 708, 796, 1147
CviAII CATG 9 cut(s) 148, 210, 271, 396, 721, 758, 838, 1021, 1174
CviQI GTAC 4 cut(s) 151, 708, 796, 1147
DdeI CTNAG 6 cut(s) 366, 411, 566, 652, 940, 1196
DpnI GATC 2 cut(s) 681, 773
DpnII GATC 2 cut(s) 679, 771
DraI TTTAAA 1 cut(s) 861
DraIII CACNNNGTG 1 cut(s) 1092
DrdI GACNNNNNNGTC 1 cut(s) 637
DseDI GACNNNNNNGTC 1 cut(s) 637
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 2 cut(s) 375, 598
Eco24I GRGCYC 1 cut(s) 321
Eco32I GATATC 1 cut(s) 812
Eco47I GGWCC 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 696, 1020
Eco81I CCTNAGG 1 cut(s) 1196
EcoICRI GAGCTC 1 cut(s) 319
EcoRV GATATC 1 cut(s) 812
EcoT14I CCWWGG 2 cut(s) 375, 598
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 2 cut(s) 375, 598
Esp3I CGTCTC 1 cut(s) 580
FaeI CATG 9 cut(s) 151, 213, 274, 399, 724, 761, 841, 1024, 1177
FalI AAGNNNNNCTT 2 cut(s) 571, 603
FaqI GGGAC 1 cut(s) 488
FatI CATG 9 cut(s) 147, 209, 270, 395, 720, 757, 837, 1020, 1173
FbaI TGATCA 1 cut(s) 771
Fnu4HI GCNGC 4 cut(s) 40, 192, 452, 731
FokI GGATG 1 cut(s) 118
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 4 cut(s) 40, 192, 452, 731
FspBI CTAG 2 cut(s) 683, 959
GlaI GCGC 2 cut(s) 344, 346
GluI GCNGC 4 cut(s) 40, 192, 452, 731
GsaI CCCAGC 1 cut(s) 20
HaeIII GGCC 1 cut(s) 1019
HapII CCGG 2 cut(s) 36, 59
HgaI GACGC 1 cut(s) 562
HhaI GCGC 2 cut(s) 345, 347
Hin1II CATG 9 cut(s) 151, 213, 274, 399, 724, 761, 841, 1024, 1177
Hin6I GCGC 2 cut(s) 343, 345
HinP1I GCGC 2 cut(s) 343, 345
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 5 cut(s) 238, 371, 419, 955, 1052
HpaII CCGG 2 cut(s) 36, 59
HphI GGTGA 2 cut(s) 247, 535
Hpy166II GTNNAC 3 cut(s) 52, 112, 984
Hpy188I TCNGA 5 cut(s) 412, 630, 715, 776, 943
Hpy188III TCNNGA 1 cut(s) 368
Hpy8I GTNNAC 3 cut(s) 52, 112, 984
HpyAV CCTTC 5 cut(s) 230, 412, 421, 710, 1146
HpyCH4III ACNGT 1 cut(s) 639
HpyCH4IV ACGT 1 cut(s) 1067
HpyCH4V TGCA 8 cut(s) 209, 399, 454, 733, 754, 761, 1103, 1213
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 131, 579, 1109
HpyF3I CTNAG 6 cut(s) 366, 411, 566, 652, 940, 1196
HpySE526I ACGT 1 cut(s) 1067
Hsp92II CATG 9 cut(s) 151, 213, 274, 399, 724, 761, 841, 1024, 1177
HspAI GCGC 2 cut(s) 343, 345
Ksp22I TGATCA 1 cut(s) 771
Kzo9I GATC 2 cut(s) 679, 771
LmnI GCTCC 4 cut(s) 356, 570, 779, 976
Lsp1109I GCAGC 3 cut(s) 178, 438, 717
LweI GCATC 4 cut(s) 39, 178, 368, 763
MaeI CTAG 2 cut(s) 683, 959
MaeII ACGT 1 cut(s) 1067
MaeIII GTNAC 3 cut(s) 72, 639, 1063
MalI GATC 2 cut(s) 681, 773
MboI GATC 2 cut(s) 679, 771
MboII GAAGA 6 cut(s) 616, 689, 1013, 1016, 1023, 1063
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 2 cut(s) 8, 321
MluCI AATT 6 cut(s) 10, 161, 518, 1162, 1214, 1219
MlyI GAGTC 2 cut(s) 365, 413
MmeI TCCRAC 1 cut(s) 450
MroXI GAANNNNTTC 2 cut(s) 242, 266
MseI TTAA 6 cut(s) 611, 687, 860, 1032, 1217, 1234
MspA1I CMGCKG 1 cut(s) 451
MspI CCGG 2 cut(s) 36, 59
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 4 cut(s) 36, 131, 579, 1109
NdeII GATC 2 cut(s) 679, 771
NlaIII CATG 9 cut(s) 151, 213, 274, 399, 724, 761, 841, 1024, 1177
NmuCI GTSAC 2 cut(s) 72, 639
NspI RCATGY 3 cut(s) 151, 213, 841
PaeI GCATGC 1 cut(s) 213
PauI GCGCGC 1 cut(s) 343
PciI ACATGT 1 cut(s) 147
PdmI GAANNNNTTC 2 cut(s) 242, 266
PfeI GAWTC 3 cut(s) 238, 955, 1052
PkrI GCNGC 4 cut(s) 41, 193, 453, 732
PleI GAGTC 2 cut(s) 365, 413
PpsI GAGTC 2 cut(s) 365, 413
PscI ACATGT 1 cut(s) 147
PsiI TTATAA 1 cut(s) 851
Psp124BI GAGCTC 1 cut(s) 321
Psp1406I AACGTT 1 cut(s) 1067
PspFI CCCAGC 1 cut(s) 16
PspPI GGNCC 1 cut(s) 217
PsuI RGATCY 1 cut(s) 679
PteI GCGCGC 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 451
RsaI GTAC 4 cut(s) 152, 709, 797, 1148
RsaNI GTAC 4 cut(s) 151, 708, 796, 1147
SacI GAGCTC 1 cut(s) 321
SaqAI TTAA 6 cut(s) 611, 687, 860, 1032, 1217, 1234
SatI GCNGC 4 cut(s) 40, 192, 452, 731
Sau3AI GATC 2 cut(s) 679, 771
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 2 cut(s) 365, 413
SduI GDGCHC 2 cut(s) 8, 321
SfaNI GCATC 4 cut(s) 39, 178, 368, 763
SfcI CTRYAG 1 cut(s) 823
SinI GGWCC 1 cut(s) 217
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
SphI GCATGC 1 cut(s) 213
Sse9I AATT 6 cut(s) 10, 161, 518, 1162, 1214, 1219
SsiI CCGC 3 cut(s) 39, 42, 327
SspMI CTAG 2 cut(s) 683, 959
SstI GAGCTC 1 cut(s) 321
StyI CCWWGG 2 cut(s) 375, 598
TaaI ACNGT 1 cut(s) 639
TaiI ACGT 1 cut(s) 1070
TaqI TCGA 1 cut(s) 168
TaqII GACCGA 1 cut(s) 1023
TasI AATT 6 cut(s) 10, 161, 518, 1162, 1214, 1219
TatI WGTACW 3 cut(s) 707, 795, 1146
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 3 cut(s) 238, 955, 1052
Tru1I TTAA 6 cut(s) 611, 687, 860, 1032, 1217, 1234
Tru9I TTAA 6 cut(s) 611, 687, 860, 1032, 1217, 1234
TscAI CASTG 1 cut(s) 1093
TseFI GTSAC 2 cut(s) 72, 639
TseI GCWGC 3 cut(s) 191, 451, 730
Tsp45I GTSAC 2 cut(s) 72, 639
TspDTI ATGAA 8 cut(s) 259, 498, 554, 968, 981, 1037, 1063, 1087
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 1 cut(s) 1093
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 2 cut(s) 1162, 1219
XceI RCATGY 3 cut(s) 151, 213, 841
XmnI GAANNNNTTC 2 cut(s) 242, 266
XspI CTAG 2 cut(s) 683, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.