MD13G1059400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012045
Physical Location & Seq
Forward (+)
4160676 .. 4162150
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1059400.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGTCCATGATTAGTACAGGGGTGCTTTCAGCAGACCCCGGAGCTGATAAGATACACAAGAAATTGTTCCACCACAGGAACGACTCCGGCGAGCTCGATGTTTTTGAAGCTGCAAGGTACTTCTCAGGATACAATGAAGCTCCCAGCAGCCATAAAAACAACAAGACATCGGCATTTTCGCAGAGGATGATGAAAGAAGACAGATCGTCATGGAGAGGAGGCAGAATCAGCCTTGACATGCCTATCAGACACATGCTCCATCATCCTCAGCAAAACCCTAATCACCATAACCAAGTGGCGATGGAAAAGCAAAGCAACATCAAAGACAAGAAGTACAAGCAACCAAGCTCTCCAGGCGGGAGGCTGGCCAGCTTCCTGAATTCTCTATTCAACCAATCGGCGTCCAAGAAGAAGAAGTCGAAATCCAGCGCCACACAGTCCATGAAAGATGAGGAGGAGAGCCCGGGTGGGAGAAGGAAGAGGAGGAGCAGCATTAGTCATTTTCGAAGCTCTAGCACCACGGATGCCAAGTCCGTGTATTCCTCATCCAGTTCAGGATTTCGAACACCTCCACCTTATAGCCATGCTCAAATGGTAGCCTCCAATAGCTACAAAGATTTGAGAAGCTATTCGGATAATCACGAGCAGCAATATCAACAGCAGCAGCAGCAGCAAACGGTTTCCTTATCAAAGTACAATACGAACTTCGATGAGAAAAGATCATCAAACAAAGAACTGACTTGGTTGGATCATGAGAAGTTCAAATTATCGGAGAAGTATAAGATTAGTTCCGACCAGGACCACAAGGGTTTGCTCAGGAGGCTCAGTGAGGTTGTTGATGACGAGCGGGAAGATGAAGGTGCAGAGAGTGATTCGAGCTCTGATCTTTTCGAGCTGCAGAATTATGACTTGGGTTATTACTCGAGCGGCCTTCCTGTGTACGAAACTACAAATGTAGACAACATCAAGATCAGATCAGTAACACCAATTTCCAATGCCTCATCCTAA

Protein Analysis

336

Amino Acids

38.1

Weight (kDa)

9.21

Isoelectric Point (pI)

65.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69160
fragaria_vesca FvH4_4g29630
malus_domestica MD13G1059400.v1.1 MD16G1058700.v1.1
prunus_persica Prupe.1G290400_v2.0.a1
pyrus_communis pycom13g05290 pycom16g05130
rosa_chinensis RchiOBHm_Chr4g0437861
rosa_laevigata RLG00000006406
rosa_multiflora Rmu_co8467433.1_g000001 Rmu_sc0008907.1_g000004
rosa_roxburghii Rroxscaffold_5G00379040
rosa_rugosa Rorug04G0304800
rosa_samantha Rh4AG356800 Rh4BG367600 Rh4CG381600 Rh4DG362100
rosa_wichuraiana Rw0G011590 Rw0G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 847, 927
AccI GTMKAC 1 cut(s) 957
AciI CCGC 3 cut(s) 357, 847, 927
AclWI GGATC 1 cut(s) 756
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 379
AcyI GRCGYC 1 cut(s) 401
AfaI GTAC 5 cut(s) 16, 119, 335, 695, 941
AgsI TTSAA 3 cut(s) 107, 391, 763
AhdI GACNNNNNGTC 1 cut(s) 205
AjnI CCWGG 2 cut(s) 352, 795
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
Alw21I GWGCWC 2 cut(s) 96, 881
AlwI GGATC 1 cut(s) 756
Ama87I CYCGRG 2 cut(s) 463, 922
AoxI GGCC 2 cut(s) 366, 928
ApeKI GCWGC 9 cut(s) 110, 147, 489, 646, 661, 664, 667, 670, 895
ApoI RAATTY 1 cut(s) 379
Asp700I GAANNNNTTC 2 cut(s) 65, 628
AspLEI GCGC 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 799
AsuC2I CCSGG 3 cut(s) 39, 464, 465
AsuHPI GGTGA 1 cut(s) 275
AsuII TTCGAA 2 cut(s) 505, 562
AvaI CYCGRG 2 cut(s) 463, 922
AvaII GGWCC 1 cut(s) 799
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 3 cut(s) 96, 464, 881
BauI CACGAG 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 2 cut(s) 96, 881
BbvCI CCTCAGC 1 cut(s) 267
BbvI GCAGC 9 cut(s) 97, 159, 501, 658, 673, 676, 679, 682, 882
BccI CCATC 2 cut(s) 267, 295
BciT130I CCWGG 2 cut(s) 354, 797
BciVI GTATCC 1 cut(s) 122
BcnI CCSGG 3 cut(s) 39, 464, 465
BfaI CTAG 1 cut(s) 513
BfmI CTRYAG 1 cut(s) 896
BfoI RGCGCY 1 cut(s) 432
BfuI GTATCC 1 cut(s) 122
Bme1390I CCNGG 5 cut(s) 39, 354, 464, 465, 797
Bme18I GGWCC 1 cut(s) 799
BmeRI GACNNNNNGTC 1 cut(s) 205
BmeT110I CYCGRG 2 cut(s) 463, 922
BmgT120I GGNCC 1 cut(s) 799
BmrFI CCNGG 5 cut(s) 39, 354, 464, 465, 797
BmsI GCATC 1 cut(s) 514
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 2 cut(s) 267, 815
Bpu14I TTCGAA 2 cut(s) 505, 562
BpuMI CCSGG 3 cut(s) 39, 464, 465
BsaHI GRCGYC 1 cut(s) 401
BsaJI CCNNGG 3 cut(s) 37, 463, 519
BsaXI ACNNNNNCTCC 4 cut(s) 240, 270, 811, 841
Bse1I ACTGG 1 cut(s) 549
BseBI CCWGG 2 cut(s) 354, 797
BseDI CCNNGG 3 cut(s) 37, 463, 519
BseGI GGATG 5 cut(s) 192, 262, 529, 545, 1001
BseMII CTCAG 4 cut(s) 138, 281, 829, 838
BseNI ACTGG 1 cut(s) 549
BseRI GAGGAG 5 cut(s) 231, 467, 470, 496, 499
BseXI GCAGC 9 cut(s) 97, 159, 501, 658, 673, 676, 679, 682, 882
BseYI CCCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 882
BshFI GGCC 2 cut(s) 368, 930
BsiHKAI GWGCWC 2 cut(s) 96, 881
BsiHKCI CYCGRG 2 cut(s) 463, 922
BsiSI CCGG 3 cut(s) 39, 87, 464
BsnI GGCC 2 cut(s) 368, 930
BsoBI CYCGRG 2 cut(s) 463, 922
Bsp119I TTCGAA 2 cut(s) 505, 562
Bsp1286I GDGCHC 3 cut(s) 96, 464, 881
Bsp143I GATC 6 cut(s) 203, 719, 748, 883, 969, 974
BspACI CCGC 3 cut(s) 357, 847, 927
BspANI GGCC 2 cut(s) 368, 930
BspCNI CTCAG 4 cut(s) 137, 280, 828, 837
BspHI TCATGA 1 cut(s) 751
BspMAI CTGCAG 1 cut(s) 900
BspPI GGATC 1 cut(s) 756
BspT104I TTCGAA 2 cut(s) 505, 562
BsrBI CCGCTC 2 cut(s) 847, 927
BsrI ACTGG 1 cut(s) 549
BssECI CCNNGG 3 cut(s) 37, 463, 519
BssMI GATC 6 cut(s) 203, 719, 748, 883, 969, 974
BssNI GRCGYC 1 cut(s) 401
BssSI CACGAG 1 cut(s) 641
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 2 cut(s) 354, 797
Bst4CI ACNGT 2 cut(s) 438, 679
Bst6I CTCTTC 1 cut(s) 473
BstACI GRCGYC 1 cut(s) 401
BstBI TTCGAA 2 cut(s) 505, 562
BstC8I GCNNGC 3 cut(s) 92, 366, 370
BstDEI CTNAG 4 cut(s) 124, 267, 815, 824
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 5 cut(s) 192, 262, 529, 545, 1001
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 6 cut(s) 206, 722, 751, 886, 972, 977
BstMBI GATC 6 cut(s) 203, 719, 748, 883, 969, 974
BstMWI GCNNNNNNNGC 5 cut(s) 228, 354, 667, 670, 820
BstNI CCWGG 2 cut(s) 354, 797
BstNSI RCATGY 2 cut(s) 241, 256
BstSCI CCNGG 5 cut(s) 37, 352, 462, 463, 795
BstSFI CTRYAG 1 cut(s) 896
BstV1I GCAGC 9 cut(s) 97, 159, 501, 658, 673, 676, 679, 682, 882
BstV2I GAAGAC 1 cut(s) 204
BsuI GTATCC 1 cut(s) 122
BsuRI GGCC 2 cut(s) 368, 930
BtgI CCRYGG 1 cut(s) 519
BtgZI GCGATG 1 cut(s) 314
BtsCI GGATG 5 cut(s) 192, 262, 529, 545, 1001
BtsIMutI CAGTG 1 cut(s) 832
Cac8I GCNNGC 3 cut(s) 92, 366, 370
CciI TCATGA 1 cut(s) 751
CfoI GCGC 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 799
Cfr9I CCCGGG 1 cut(s) 463
CseI GACGC 1 cut(s) 390
Csp6I GTAC 5 cut(s) 15, 118, 334, 694, 940
CviAII CATG 7 cut(s) 7, 210, 238, 253, 442, 584, 752
CviQI GTAC 5 cut(s) 15, 118, 334, 694, 940
DdeI CTNAG 4 cut(s) 124, 267, 815, 824
DpnI GATC 6 cut(s) 205, 721, 750, 885, 971, 976
DpnII GATC 6 cut(s) 203, 719, 748, 883, 969, 974
DriI GACNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 473
Eam1105I GACNNNNNGTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 473
Ecl136II GAGCTC 2 cut(s) 94, 879
Eco24I GRGCYC 3 cut(s) 96, 464, 881
Eco47I GGWCC 1 cut(s) 799
Eco53kI GAGCTC 2 cut(s) 94, 879
Eco88I CYCGRG 2 cut(s) 463, 922
EcoICRI GAGCTC 2 cut(s) 94, 879
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 352, 795
EcoT38I GRGCYC 3 cut(s) 96, 464, 881
FaeI CATG 7 cut(s) 10, 213, 241, 256, 445, 587, 755
FatI CATG 7 cut(s) 6, 209, 237, 252, 441, 583, 751
FauI CCCGC 2 cut(s) 350, 840
FblI GTMKAC 1 cut(s) 957
FokI GGATG 5 cut(s) 199, 249, 532, 536, 988
FriOI GRGCYC 3 cut(s) 96, 464, 881
FspBI CTAG 1 cut(s) 513
GlaI GCGC 1 cut(s) 430
GsaI CCCAGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 432
HaeIII GGCC 2 cut(s) 368, 930
HapII CCGG 3 cut(s) 39, 87, 464
HgaI GACGC 1 cut(s) 390
HhaI GCGC 1 cut(s) 431
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 7 cut(s) 10, 213, 241, 256, 445, 587, 755
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HinfI GANTC 3 cut(s) 83, 225, 872
HpaII CCGG 3 cut(s) 39, 87, 464
HphI GGTGA 1 cut(s) 275
Hpy166II GTNNAC 2 cut(s) 940, 958
Hpy188I TCNGA 6 cut(s) 248, 634, 772, 793, 883, 974
Hpy188III TCNNGA 7 cut(s) 126, 376, 555, 641, 752, 817, 967
Hpy8I GTNNAC 2 cut(s) 940, 958
HpyAV CCTTC 3 cut(s) 468, 851, 941
HpyCH4III ACNGT 2 cut(s) 438, 679
HpyCH4V TGCA 3 cut(s) 113, 863, 898
HpyF10VI GCNNNNNNNGC 5 cut(s) 228, 354, 667, 670, 820
HpyF3I CTNAG 4 cut(s) 124, 267, 815, 824
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 7 cut(s) 10, 213, 241, 256, 445, 587, 755
HspAI GCGC 1 cut(s) 429
Kzo9I GATC 6 cut(s) 203, 719, 748, 883, 969, 974
LmnI GCTCC 4 cut(s) 41, 145, 261, 486
Lsp1109I GCAGC 9 cut(s) 97, 159, 501, 658, 673, 676, 679, 682, 882
LweI GCATC 1 cut(s) 514
MaeI CTAG 1 cut(s) 513
MaeIII GTNAC 1 cut(s) 979
MalI GATC 6 cut(s) 205, 721, 750, 885, 971, 976
MbiI CCGCTC 2 cut(s) 847, 927
MboI GATC 6 cut(s) 203, 719, 748, 883, 969, 974
MboII GAAGA 5 cut(s) 209, 421, 424, 490, 863
MhlI GDGCHC 3 cut(s) 96, 464, 881
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 5 cut(s) 62, 379, 764, 901, 987
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 2 cut(s) 726, 816
Mox20I TGGCCA 1 cut(s) 368
MroXI GAANNNNTTC 2 cut(s) 65, 628
MscI TGGCCA 1 cut(s) 368
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 3 cut(s) 39, 87, 464
MspR9I CCNGG 5 cut(s) 39, 354, 464, 465, 797
MvaI CCWGG 2 cut(s) 354, 797
MwoI GCNNNNNNNGC 5 cut(s) 228, 354, 667, 670, 820
NciI CCSGG 3 cut(s) 39, 464, 465
NdeII GATC 6 cut(s) 203, 719, 748, 883, 969, 974
NlaIII CATG 7 cut(s) 10, 213, 241, 256, 445, 587, 755
NspI RCATGY 2 cut(s) 241, 256
NspV TTCGAA 2 cut(s) 505, 562
PaeR7I CTCGAG 1 cut(s) 922
PagI TCATGA 1 cut(s) 751
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdmI GAANNNNTTC 2 cut(s) 65, 628
PfeI GAWTC 2 cut(s) 225, 872
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 2 cut(s) 96, 881
Psp6I CCWGG 2 cut(s) 352, 795
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 352, 795
PspPI GGNCC 1 cut(s) 799
PspXI VCTCGAGB 1 cut(s) 922
PstI CTGCAG 1 cut(s) 900
RsaI GTAC 5 cut(s) 16, 119, 335, 695, 941
RsaNI GTAC 5 cut(s) 15, 118, 334, 694, 940
SacI GAGCTC 2 cut(s) 96, 881
Sau3AI GATC 6 cut(s) 203, 719, 748, 883, 969, 974
Sau96I GGNCC 1 cut(s) 799
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 5 cut(s) 39, 354, 464, 465, 797
SduI GDGCHC 3 cut(s) 96, 464, 881
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 896
Sfr274I CTCGAG 1 cut(s) 922
SfuI TTCGAA 2 cut(s) 505, 562
SinI GGWCC 1 cut(s) 799
SlaI CTCGAG 1 cut(s) 922
SmaI CCCGGG 1 cut(s) 465
SmlI CTYRAG 1 cut(s) 922
SmoI CTYRAG 1 cut(s) 922
Sse9I AATT 5 cut(s) 62, 379, 764, 901, 987
SsiI CCGC 3 cut(s) 357, 847, 927
SspMI CTAG 1 cut(s) 513
SstI GAGCTC 2 cut(s) 96, 881
StyD4I CCNGG 5 cut(s) 37, 352, 462, 463, 795
TaaI ACNGT 2 cut(s) 438, 679
TaqI TCGA 8 cut(s) 96, 419, 505, 562, 708, 875, 891, 923
TasI AATT 5 cut(s) 62, 379, 764, 901, 987
TatI WGTACW 3 cut(s) 14, 333, 693
TauI GCSGC 1 cut(s) 930
TfiI GAWTC 2 cut(s) 225, 872
TscAI CASTG 1 cut(s) 832
TseI GCWGC 9 cut(s) 110, 147, 489, 646, 661, 664, 667, 670, 895
TspDTI ATGAA 4 cut(s) 150, 206, 458, 870
TspGWI ACGGA 2 cut(s) 523, 536
TspMI CCCGGG 1 cut(s) 463
TspRI CASTG 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 799
XapI RAATTY 1 cut(s) 379
XceI RCATGY 2 cut(s) 241, 256
XhoI CTCGAG 1 cut(s) 922
XmaI CCCGGG 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 957
XmnI GAANNNNTTC 2 cut(s) 65, 628
XspI CTAG 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.