MD16G1058700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012045
Physical Location & Seq
Forward (+)
4197128 .. 4198205
1078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1058700.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGTCTATTATTAGTACAGGGGTGCTCTCGGCAGACCCTGGAGCTGATAAGATACACAAGAAATCGTTCCACCACAGGAACGACTCCGGGGAGCTCGATGTTTTCGAAGCTGCAAGGTACTTCTCAGGATACATTGAAGCTCCCAGCAGCCATAACAACAACAGCAGCAGCAACACTTCGGCATTTTCGCAGAGGATGATGAAAGAAGACAGATCTTCATGGAGAGGAGGCAGAATCAGCCTTGATATGCCTATAAGACACATGCTCCATCATCCTCAGCAAAACCCTAATCACCATAACCACGTGGCGGTGGAAAAGCAAAACAACATCAAAGACAAGAAATACAAGCAGCCGAGCTCTCCAGGCGGGAGACTAGCCAGCTTCTTGAATTCTCTATTCAACCAATCAGCATCCAAGAAGAAGAAGTCAAAATCCAGCGCCACGCAGTCCATGAAAGACGAGGAAGAGAGCCCAGGTGGGAGGCGGAGGAGGAGGAGCAGCATTAGTCATTTTCGAAGCTCGAGCACCACGGATGCCAAGTCCGTGTATTCCTCATCGAGTTCAGGGTTTCGAACACCTCCTCCTTATAGCCATGCACAAACGGCAGCCTCCAAGAGCTACAAAGATTTGAGAAGCTATTCGGATAATCACAAGCAGCAATATCAACAGCAGCAGCAAGCGGTTTCTTTATCGAAGTACAATACGAACTTCGATGACAAAAGATCATCAAACAAAGAACTGACTTGGTTGGATCATGAGAAGTTCAAATTATCGGAGAAGTATAAGATTAGTTCCGAGCTAGATCACAAGGGTTTGCTCAAAAGGCTAAGTGAGGTTGTTGACGACGAGGAGGAAGATGAAGGTGCAGAGAGTGATTCGAGCTCTGATCTTTTCGAGCTGCAGAATTATGACCTGGGTTTTTACTCGAGCGGCTTGCCTGTGTACGAAACTACAAATGTGGACAACATCAAGATCAGATCAGGAACACCAATTTCCAATGCTTCGTCCTAA

Protein Analysis

337

Amino Acids

37.86

Weight (kDa)

9.13

Isoelectric Point (pI)

69.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69160
fragaria_vesca FvH4_4g29630
malus_domestica MD13G1059400.v1.1 MD16G1058700.v1.1
prunus_persica Prupe.1G290400_v2.0.a1
pyrus_communis pycom13g05290 pycom16g05130
rosa_chinensis RchiOBHm_Chr4g0437861
rosa_laevigata RLG00000006406
rosa_multiflora Rmu_co8467433.1_g000001 Rmu_sc0008907.1_g000004
rosa_roxburghii Rroxscaffold_5G00379040
rosa_rugosa Rorug04G0304800
rosa_samantha Rh4AG356800 Rh4BG367600 Rh4CG381600 Rh4DG362100
rosa_wichuraiana Rw0G011590 Rw0G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 930
AciI CCGC 5 cut(s) 308, 366, 484, 680, 930
AclWI GGATC 1 cut(s) 759
AcsI RAATTY 1 cut(s) 388
AcvI CACGTG 1 cut(s) 304
AfaI GTAC 4 cut(s) 16, 119, 698, 944
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 4 cut(s) 137, 388, 400, 766
AjnI CCWGG 4 cut(s) 37, 361, 472, 912
AloI GAACNNNNNNTCC 2 cut(s) 565, 597
Alw21I GWGCWC 5 cut(s) 27, 96, 359, 527, 884
Alw26I GTCTC 1 cut(s) 364
AlwI GGATC 1 cut(s) 759
AlwNI CAGNNNCTG 1 cut(s) 38
Ama87I CYCGRG 2 cut(s) 520, 925
ApoI RAATTY 1 cut(s) 388
Asp700I GAANNNNTTC 2 cut(s) 65, 637
AspLEI GCGC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 284
AsuII TTCGAA 3 cut(s) 105, 514, 571
AvaI CYCGRG 2 cut(s) 520, 925
BanII GRGCYC 4 cut(s) 96, 359, 473, 884
BbrPI CACGTG 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 5 cut(s) 27, 96, 359, 527, 884
BbvCI CCTCAGC 1 cut(s) 276
BccI CCATC 1 cut(s) 276
BceAI ACGGC 1 cut(s) 618
BcgI CGANNNNNNTGC 2 cut(s) 916, 950
BciT130I CCWGG 4 cut(s) 39, 363, 474, 914
BciVI GTATCC 1 cut(s) 122
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 364
BfaI CTAG 2 cut(s) 374, 800
BfmI CTRYAG 1 cut(s) 899
BfoI RGCGCY 1 cut(s) 441
BfuI GTATCC 1 cut(s) 122
BglII AGATCT 1 cut(s) 212
Bme1390I CCNGG 5 cut(s) 39, 88, 363, 474, 914
BmeT110I CYCGRG 2 cut(s) 520, 925
BmrFI CCNGG 5 cut(s) 39, 88, 363, 474, 914
BmsI GCATC 2 cut(s) 419, 523
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 2 cut(s) 60, 345
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 3 cut(s) 105, 514, 571
BpuMI CCSGG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 304
BsaJI CCNNGG 5 cut(s) 37, 87, 472, 528, 913
BsaXI ACNNNNNCTCC 4 cut(s) 249, 279, 565, 595
Bsc4I CCNNNNNNNGG 1 cut(s) 307
BseBI CCWGG 4 cut(s) 39, 363, 474, 914
BseDI CCNNGG 5 cut(s) 37, 87, 472, 528, 913
BseGI GGATG 4 cut(s) 201, 271, 410, 538
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMII CTCAG 2 cut(s) 138, 290
BseRI GAGGAG 6 cut(s) 240, 502, 505, 508, 570, 863
BseYI CCCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 885
BsiHKAI GWGCWC 5 cut(s) 27, 96, 359, 527, 884
BsiHKCI CYCGRG 2 cut(s) 520, 925
BsiSI CCGG 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 364
BsoBI CYCGRG 2 cut(s) 520, 925
Bsp119I TTCGAA 3 cut(s) 105, 514, 571
Bsp1286I GDGCHC 6 cut(s) 27, 96, 359, 473, 527, 884
Bsp143I GATC 7 cut(s) 212, 722, 751, 802, 886, 972, 977
BspACI CCGC 5 cut(s) 308, 366, 484, 680, 930
BspCNI CTCAG 2 cut(s) 137, 289
BspHI TCATGA 1 cut(s) 754
BspMAI CTGCAG 1 cut(s) 903
BspPI GGATC 1 cut(s) 759
BspT104I TTCGAA 3 cut(s) 105, 514, 571
BsrBI CCGCTC 1 cut(s) 930
BssECI CCNNGG 5 cut(s) 37, 87, 472, 528, 913
BssMI GATC 7 cut(s) 212, 722, 751, 802, 886, 972, 977
Bst2UI CCWGG 4 cut(s) 39, 363, 474, 914
Bst6I CTCTTC 1 cut(s) 459
BstBAI YACGTR 1 cut(s) 304
BstBI TTCGAA 3 cut(s) 105, 514, 571
BstC8I GCNNGC 3 cut(s) 379, 678, 935
BstDEI CTNAG 3 cut(s) 124, 276, 827
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 4 cut(s) 201, 271, 410, 538
BstH2I RGCGCY 1 cut(s) 441
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 7 cut(s) 215, 725, 754, 805, 889, 975, 980
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 7 cut(s) 212, 722, 751, 802, 886, 972, 977
BstMWI GCNNNNNNNGC 4 cut(s) 237, 363, 602, 823
BstNI CCWGG 4 cut(s) 39, 363, 474, 914
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 5 cut(s) 37, 86, 361, 472, 912
BstSFI CTRYAG 1 cut(s) 899
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 212
BstYI RGATCY 1 cut(s) 212
BsuI GTATCC 1 cut(s) 122
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 4 cut(s) 201, 271, 410, 538
Cac8I GCNNGC 3 cut(s) 379, 678, 935
CaiI CAGNNNCTG 1 cut(s) 38
CciI TCATGA 1 cut(s) 754
CfoI GCGC 1 cut(s) 440
Csp6I GTAC 4 cut(s) 15, 118, 697, 943
CviAII CATG 5 cut(s) 219, 262, 451, 593, 755
CviQI GTAC 4 cut(s) 15, 118, 697, 943
DdeI CTNAG 3 cut(s) 124, 276, 827
DpnI GATC 7 cut(s) 214, 724, 753, 804, 888, 974, 979
DpnII GATC 7 cut(s) 212, 722, 751, 802, 886, 972, 977
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 499
Ecl136II GAGCTC 3 cut(s) 94, 357, 882
Eco24I GRGCYC 4 cut(s) 96, 359, 473, 884
Eco53kI GAGCTC 3 cut(s) 94, 357, 882
Eco72I CACGTG 1 cut(s) 304
Eco88I CYCGRG 2 cut(s) 520, 925
EcoICRI GAGCTC 3 cut(s) 94, 357, 882
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 4 cut(s) 37, 361, 472, 912
EcoT38I GRGCYC 4 cut(s) 96, 359, 473, 884
FaeI CATG 5 cut(s) 222, 265, 454, 596, 758
FatI CATG 5 cut(s) 218, 261, 450, 592, 754
FauI CCCGC 1 cut(s) 359
FokI GGATG 4 cut(s) 208, 258, 397, 545
FriOI GRGCYC 4 cut(s) 96, 359, 473, 884
FspBI CTAG 2 cut(s) 374, 800
GlaI GCGC 1 cut(s) 439
GsaI CCCAGC 1 cut(s) 147
GsuI CTGGAG 2 cut(s) 60, 345
HaeII RGCGCY 1 cut(s) 441
HapII CCGG 1 cut(s) 87
HhaI GCGC 1 cut(s) 440
Hin1II CATG 5 cut(s) 222, 265, 454, 596, 758
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HinfI GANTC 3 cut(s) 83, 234, 875
HpaII CCGG 1 cut(s) 87
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 3 cut(s) 841, 943, 961
Hpy188I TCNGA 5 cut(s) 643, 775, 796, 886, 977
Hpy188III TCNNGA 5 cut(s) 126, 385, 755, 970, 981
Hpy8I GTNNAC 3 cut(s) 841, 943, 961
Hpy99I CGWCG 1 cut(s) 848
HpyAV CCTTC 1 cut(s) 854
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 4 cut(s) 113, 596, 866, 901
HpyF10VI GCNNNNNNNGC 4 cut(s) 237, 363, 602, 823
HpyF3I CTNAG 3 cut(s) 124, 276, 827
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 5 cut(s) 222, 265, 454, 596, 758
HspAI GCGC 1 cut(s) 438
Kzo9I GATC 7 cut(s) 212, 722, 751, 802, 886, 972, 977
LmnI GCTCC 5 cut(s) 41, 91, 145, 270, 495
LweI GCATC 2 cut(s) 419, 523
MaeI CTAG 2 cut(s) 374, 800
MaeII ACGT 1 cut(s) 303
MalI GATC 7 cut(s) 214, 724, 753, 804, 888, 974, 979
MbiI CCGCTC 1 cut(s) 930
MboI GATC 7 cut(s) 212, 722, 751, 802, 886, 972, 977
MboII GAAGA 6 cut(s) 207, 218, 430, 433, 476, 866
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 6 cut(s) 27, 96, 359, 473, 527, 884
MluCI AATT 4 cut(s) 388, 767, 904, 990
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 729
MroXI GAANNNNTTC 2 cut(s) 65, 637
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 5 cut(s) 39, 88, 363, 474, 914
MvaI CCWGG 4 cut(s) 39, 363, 474, 914
MwoI GCNNNNNNNGC 4 cut(s) 237, 363, 602, 823
NciI CCSGG 1 cut(s) 88
NdeII GATC 7 cut(s) 212, 722, 751, 802, 886, 972, 977
NlaIII CATG 5 cut(s) 222, 265, 454, 596, 758
NmeAIII GCCGAG 2 cut(s) 8, 378
NspI RCATGY 1 cut(s) 265
NspV TTCGAA 3 cut(s) 105, 514, 571
PaeR7I CTCGAG 2 cut(s) 520, 925
PagI TCATGA 1 cut(s) 754
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 2 cut(s) 65, 637
PfeI GAWTC 2 cut(s) 234, 875
PleI GAGTC 1 cut(s) 77
PmaCI CACGTG 1 cut(s) 304
PmlI CACGTG 1 cut(s) 304
PpsI GAGTC 1 cut(s) 77
Ppu21I YACGTR 1 cut(s) 304
Psp124BI GAGCTC 3 cut(s) 96, 359, 884
Psp6I CCWGG 4 cut(s) 37, 361, 472, 912
PspCI CACGTG 1 cut(s) 304
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 4 cut(s) 37, 361, 472, 912
PspXI VCTCGAGB 2 cut(s) 520, 925
PstI CTGCAG 1 cut(s) 903
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 1 cut(s) 212
RsaI GTAC 4 cut(s) 16, 119, 698, 944
RsaNI GTAC 4 cut(s) 15, 118, 697, 943
SacI GAGCTC 3 cut(s) 96, 359, 884
Sau3AI GATC 7 cut(s) 212, 722, 751, 802, 886, 972, 977
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 5 cut(s) 39, 88, 363, 474, 914
SduI GDGCHC 6 cut(s) 27, 96, 359, 473, 527, 884
SfaNI GCATC 2 cut(s) 419, 523
SfcI CTRYAG 1 cut(s) 899
Sfr274I CTCGAG 2 cut(s) 520, 925
SfuI TTCGAA 3 cut(s) 105, 514, 571
SlaI CTCGAG 2 cut(s) 520, 925
SmlI CTYRAG 2 cut(s) 520, 925
SmoI CTYRAG 2 cut(s) 520, 925
Sse9I AATT 4 cut(s) 388, 767, 904, 990
SsiI CCGC 5 cut(s) 308, 366, 484, 680, 930
SspMI CTAG 2 cut(s) 374, 800
SstI GAGCTC 3 cut(s) 96, 359, 884
StyD4I CCNGG 5 cut(s) 37, 86, 361, 472, 912
TaiI ACGT 1 cut(s) 306
TasI AATT 4 cut(s) 388, 767, 904, 990
TatI WGTACW 2 cut(s) 14, 696
TauI GCSGC 1 cut(s) 933
TfiI GAWTC 2 cut(s) 234, 875
TspDTI ATGAA 4 cut(s) 207, 215, 467, 873
TspGWI ACGGA 2 cut(s) 532, 545
XapI RAATTY 1 cut(s) 388
XceI RCATGY 1 cut(s) 265
XhoI CTCGAG 2 cut(s) 520, 925
XmnI GAANNNNTTC 2 cut(s) 65, 637
XspI CTAG 2 cut(s) 374, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.