pycom16g05130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012045
Physical Location & Seq
Forward (+)
3314737 .. 3315301
565 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g05130.2

Sequence Viewer

Length: 522 bp
ATGAAAGACGAGGAAGAGAGCCCAGGTGGAAGACGGAGGAGGAGGAGCAGCATTAGTCATTTTCGAAGCTCGAGCACCACGGATGCCAAGTCCCTGTATTCCTCATCGAGTTCAGGGTTTCGAACACCTCCTCCTTATAGCCATGTACAAACGGCAGCCTCCAAGAGCTACAAAGATTTGAGAAGCTATTCGGATAATCACAAGCAGCAATATCAACAGCAGCAGCAAAAAGATCGATCAAACAAAGAACTGACTTGGTTGGATCATGAGAAGTTCAAATTATCGGAGAAGTATAAGATTAGTTCCGAGCTAGATCACAAGGGTTTGCTCAAAAGGCTAAGTGAGGTTGTTGACGACGAGGAGGAAGATGAAGGTGCAGAGAGTGATTCGAGCTCTGATCTTTTCGAGCGGCAGAATTATGACCTGGGTTTTTACTCGAGCGGCTTGCCTGTGTACGAAACTACAAATGTGGACAACATCAAGATCAGATCAGGAACACCAATTTCCAATGCTTCGTCCTAA

Protein Analysis

174

Amino Acids

19.78

Weight (kDa)

5.98

Isoelectric Point (pI)

83.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69160
fragaria_vesca FvH4_4g29630
malus_domestica MD13G1059400.v1.1 MD16G1058700.v1.1
prunus_persica Prupe.1G290400_v2.0.a1
pyrus_communis pycom13g05290 pycom16g05130
rosa_chinensis RchiOBHm_Chr4g0437861
rosa_laevigata RLG00000006406
rosa_multiflora Rmu_co8467433.1_g000001 Rmu_sc0008907.1_g000004
rosa_roxburghii Rroxscaffold_5G00379040
rosa_rugosa Rorug04G0304800
rosa_samantha Rh4AG356800 Rh4BG367600 Rh4CG381600 Rh4DG362100
rosa_wichuraiana Rw0G011590 Rw0G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 409, 441
AciI CCGC 2 cut(s) 409, 441
AclWI GGATC 1 cut(s) 270
AfaI GTAC 2 cut(s) 147, 455
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 2 cut(s) 22, 423
AloI GAACNNNNNNTCC 2 cut(s) 115, 147
AluBI AGCT 5 cut(s) 69, 168, 186, 310, 393
AluI AGCT 5 cut(s) 69, 168, 186, 310, 393
Alw21I GWGCWC 2 cut(s) 77, 395
AlwI GGATC 1 cut(s) 270
Ama87I CYCGRG 2 cut(s) 70, 436
ApeKI GCWGC 5 cut(s) 48, 155, 205, 220, 223
Asp700I GAANNNNTTC 1 cut(s) 187
AsuII TTCGAA 2 cut(s) 64, 121
AvaI CYCGRG 2 cut(s) 70, 436
BanII GRGCYC 2 cut(s) 23, 395
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 2 cut(s) 77, 395
BbvI GCAGC 5 cut(s) 60, 167, 217, 232, 235
BceAI ACGGC 1 cut(s) 168
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 427, 461
BciT130I CCWGG 2 cut(s) 24, 425
BfaI CTAG 1 cut(s) 311
BisI GCNGC 7 cut(s) 49, 156, 206, 221, 224, 410, 442
BlsI GCNGC 7 cut(s) 50, 157, 207, 222, 225, 411, 443
Bme1390I CCNGG 2 cut(s) 24, 425
BmeT110I CYCGRG 2 cut(s) 70, 436
BmrFI CCNGG 2 cut(s) 24, 425
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 37
Bpu14I TTCGAA 2 cut(s) 64, 121
Bsa29I ATCGAT 1 cut(s) 235
BsaJI CCNNGG 3 cut(s) 22, 78, 424
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
BseBI CCWGG 2 cut(s) 24, 425
BseCI ATCGAT 1 cut(s) 235
BseDI CCNNGG 3 cut(s) 22, 78, 424
BseGI GGATG 1 cut(s) 88
BseRI GAGGAG 5 cut(s) 52, 55, 58, 120, 374
BseXI GCAGC 5 cut(s) 60, 167, 217, 232, 235
BsgI GTGCAG 1 cut(s) 396
BshVI ATCGAT 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 77, 395
BsiHKCI CYCGRG 2 cut(s) 70, 436
BslFI GGGAC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 76
BsoBI CYCGRG 2 cut(s) 70, 436
Bsp119I TTCGAA 2 cut(s) 64, 121
Bsp1286I GDGCHC 3 cut(s) 23, 77, 395
Bsp1407I TGTACA 1 cut(s) 145
Bsp143I GATC 7 cut(s) 232, 236, 262, 313, 397, 483, 488
BspACI CCGC 2 cut(s) 409, 441
BspDI ATCGAT 1 cut(s) 235
BspHI TCATGA 1 cut(s) 265
BspPI GGATC 1 cut(s) 270
BspT104I TTCGAA 2 cut(s) 64, 121
BsrBI CCGCTC 2 cut(s) 409, 441
BsrGI TGTACA 1 cut(s) 145
BssECI CCNNGG 3 cut(s) 22, 78, 424
BssMI GATC 7 cut(s) 232, 236, 262, 313, 397, 483, 488
Bst2UI CCWGG 2 cut(s) 24, 425
Bst6I CTCTTC 1 cut(s) 9
BstAUI TGTACA 1 cut(s) 145
BstBI TTCGAA 2 cut(s) 64, 121
BstC8I GCNNGC 1 cut(s) 446
BstDEI CTNAG 1 cut(s) 338
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 1 cut(s) 88
BstKTI GATC 7 cut(s) 235, 239, 265, 316, 400, 486, 491
BstMBI GATC 7 cut(s) 232, 236, 262, 313, 397, 483, 488
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNI CCWGG 2 cut(s) 24, 425
BstSCI CCNGG 2 cut(s) 22, 423
BstV1I GCAGC 5 cut(s) 60, 167, 217, 232, 235
BstV2I GAAGAC 1 cut(s) 37
Bsu15I ATCGAT 1 cut(s) 235
BsuTUI ATCGAT 1 cut(s) 235
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 1 cut(s) 88
Cac8I GCNNGC 1 cut(s) 446
CciI TCATGA 1 cut(s) 265
ClaI ATCGAT 1 cut(s) 235
Csp6I GTAC 2 cut(s) 146, 454
CviAII CATG 2 cut(s) 143, 266
CviQI GTAC 2 cut(s) 146, 454
DdeI CTNAG 1 cut(s) 338
DpnI GATC 7 cut(s) 234, 238, 264, 315, 399, 485, 490
DpnII GATC 7 cut(s) 232, 236, 262, 313, 397, 483, 488
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Ecl136II GAGCTC 1 cut(s) 393
Eco24I GRGCYC 2 cut(s) 23, 395
Eco53kI GAGCTC 1 cut(s) 393
Eco88I CYCGRG 2 cut(s) 70, 436
EcoICRI GAGCTC 1 cut(s) 393
EcoRII CCWGG 2 cut(s) 22, 423
EcoT38I GRGCYC 2 cut(s) 23, 395
FaeI CATG 2 cut(s) 146, 269
FaiI YATR 5 cut(s) 138, 144, 267, 294, 420
FaqI GGGAC 1 cut(s) 76
FatI CATG 2 cut(s) 142, 265
Fnu4HI GCNGC 7 cut(s) 49, 156, 206, 221, 224, 410, 442
FokI GGATG 1 cut(s) 95
FriOI GRGCYC 2 cut(s) 23, 395
Fsp4HI GCNGC 7 cut(s) 49, 156, 206, 221, 224, 410, 442
FspBI CTAG 1 cut(s) 311
GluI GCNGC 7 cut(s) 49, 156, 206, 221, 224, 410, 442
Hin1II CATG 2 cut(s) 146, 269
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 1 cut(s) 386
Hpy166II GTNNAC 3 cut(s) 352, 454, 472
Hpy188I TCNGA 5 cut(s) 193, 286, 307, 397, 488
Hpy188III TCNNGA 3 cut(s) 266, 481, 492
Hpy8I GTNNAC 3 cut(s) 352, 454, 472
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 1 cut(s) 365
HpyCH4V TGCA 1 cut(s) 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 1 cut(s) 338
Hsp92II CATG 2 cut(s) 146, 269
Kzo9I GATC 7 cut(s) 232, 236, 262, 313, 397, 483, 488
LmnI GCTCC 1 cut(s) 45
LpnPI CCDG 8 cut(s) 9, 36, 99, 107, 410, 437, 462, 477
Lsp1109I GCAGC 5 cut(s) 60, 167, 217, 232, 235
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 311
MalI GATC 7 cut(s) 234, 238, 264, 315, 399, 485, 490
MbiI CCGCTC 2 cut(s) 409, 441
MboI GATC 7 cut(s) 232, 236, 262, 313, 397, 483, 488
MboII GAAGA 3 cut(s) 26, 42, 377
MhlI GDGCHC 3 cut(s) 23, 77, 395
MluCI AATT 3 cut(s) 278, 415, 501
MmeI TCCRAC 1 cut(s) 240
MroXI GAANNNNTTC 1 cut(s) 187
MspR9I CCNGG 2 cut(s) 24, 425
MvaI CCWGG 2 cut(s) 24, 425
MwoI GCNNNNNNNGC 1 cut(s) 334
NdeII GATC 7 cut(s) 232, 236, 262, 313, 397, 483, 488
NlaIII CATG 2 cut(s) 146, 269
NspV TTCGAA 2 cut(s) 64, 121
PaeR7I CTCGAG 2 cut(s) 70, 436
PagI TCATGA 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 386
PkrI GCNGC 7 cut(s) 50, 157, 207, 222, 225, 411, 443
Psp124BI GAGCTC 1 cut(s) 395
Psp6I CCWGG 2 cut(s) 22, 423
PspGI CCWGG 2 cut(s) 22, 423
PspXI VCTCGAGB 2 cut(s) 70, 436
RsaI GTAC 2 cut(s) 147, 455
RsaNI GTAC 2 cut(s) 146, 454
SacI GAGCTC 1 cut(s) 395
SatI GCNGC 7 cut(s) 49, 156, 206, 221, 224, 410, 442
Sau3AI GATC 7 cut(s) 232, 236, 262, 313, 397, 483, 488
ScrFI CCNGG 2 cut(s) 24, 425
SduI GDGCHC 3 cut(s) 23, 77, 395
SfaNI GCATC 1 cut(s) 73
Sfr274I CTCGAG 2 cut(s) 70, 436
SfuI TTCGAA 2 cut(s) 64, 121
SlaI CTCGAG 2 cut(s) 70, 436
SmlI CTYRAG 2 cut(s) 70, 436
SmoI CTYRAG 2 cut(s) 70, 436
Sse9I AATT 3 cut(s) 278, 415, 501
SsiI CCGC 2 cut(s) 409, 441
SspMI CTAG 1 cut(s) 311
SstI GAGCTC 1 cut(s) 395
StyD4I CCNGG 2 cut(s) 22, 423
TaqI TCGA 8 cut(s) 64, 71, 107, 121, 235, 389, 405, 437
TasI AATT 3 cut(s) 278, 415, 501
TatI WGTACW 1 cut(s) 145
TauI GCSGC 2 cut(s) 412, 444
TfiI GAWTC 1 cut(s) 386
TseI GCWGC 5 cut(s) 48, 155, 205, 220, 223
TspDTI ATGAA 2 cut(s) 17, 384
TspGWI ACGGA 2 cut(s) 49, 95
XhoI CTCGAG 2 cut(s) 70, 436
XmnI GAANNNNTTC 1 cut(s) 187
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.