pycom13g05290

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012045
Physical Location & Seq
Forward (+)
3526264 .. 3527539
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g05290.1

Sequence Viewer

Length: 1149 bp
ATGTCCATGATTAGTACAGGGGTGCTTTCAGCAGACCCCGGAGCTGATAAGATACACAAGAAATTGTTCCACCACAGGAACGACTCTGGCGAGCTCGATGTTTTTGAAGCTGCAAGGTACTTCTCAGGATACAATGAAACTCCCAGCAGCCATAAAAACAACAAGACTTCGGCATTTTCGCAGAGGATGATGAAAGAAGACAGATCGTCATGGAGAGGAGGCAGAATCAGCCTTGACATGCCTATCAGACACATGCTTCATCATCCTCAGCAAAACCCTAATCACCATAACCACGTGGCGATGGAAAAGCAAAGCAACATCAAAGACAAGAAGTACAAGCAACCAAGCTCTCCAGGCGGGAGGCTGGCCAGCTTCCTGAATTCTCTATTCAACCAATCAGTGTCCAAGAAGAAGAAGTCGAAATCCAGCGCCACACAGTCCATGAAAGACGAGGAGGAGAGCCCGGGTGGGAGAAGGAGGAGGAGGAGCAGCATTAGTCATTTTCGAAGCTCTAGCACCACGGATGCCAAGTCCGTGTATTCCTCATCGAGTTCAGGATTTCGAACACCTCCACCTTATAGCCATGCACAAATGGTAGCCTCCAATAGCTACAAAGATTTGAGAAGCTATTCGGATAATCACAAGCAGCAATATCAACAGCAGCAGCAGCAGAGAAAAGATCGATCAAACAAAGAACTGACTTGGTTCGATCATGAGAAGTTCAAATTATCAGAGAAATATAAGATTAGTTCCGACCAGGACCACAAGGGTTTGCTCAAGAGGCTCAGTGAGGTTGTTGATGACGAGCAGGAAGATGAAGGTGCAGAAAGTGATTCGAGCTCTGATCTTTTCGAGCTGCAGAATTATGACTTGGGTTATTACTCGAGCGGCTTGCCTGTGTACGAAACTACAAATGTAGACAACATCAAGATCAGATCAGTAACACCAATTTCCAATTATGCATATGTGGTTGAGAAAGGAATTAAAATTTCCTACATTTTTCTTTTTAAGTTCTCTTATCTTAAACTTCCATTGGCCTTTTTTATATGTATGTACAATATGTATAGGTTAAGATTTTTAGTATACTTGTATCTTATATTTCTTGATTTTTTAACAGTTAGATTCTTATATTTCATGACTAGTATCTAA

Protein Analysis

383

Amino Acids

44.34

Weight (kDa)

9.46

Isoelectric Point (pI)

60.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69160
fragaria_vesca FvH4_4g29630
malus_domestica MD13G1059400.v1.1 MD16G1058700.v1.1
prunus_persica Prupe.1G290400_v2.0.a1
pyrus_communis pycom13g05290 pycom16g05130
rosa_chinensis RchiOBHm_Chr4g0437861
rosa_laevigata RLG00000006406
rosa_multiflora Rmu_co8467433.1_g000001 Rmu_sc0008907.1_g000004
rosa_roxburghii Rroxscaffold_5G00379040
rosa_rugosa Rorug04G0304800
rosa_samantha Rh4AG356800 Rh4BG367600 Rh4CG381600 Rh4DG362100
rosa_wichuraiana Rw0G011590 Rw0G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 888
AccI GTMKAC 2 cut(s) 918, 1083
AciI CCGC 2 cut(s) 357, 888
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 2 cut(s) 379, 987
AcvI CACGTG 1 cut(s) 295
AfaI GTAC 5 cut(s) 16, 119, 335, 902, 1055
AgsI TTSAA 3 cut(s) 107, 391, 724
AhdI GACNNNNNGTC 1 cut(s) 205
AhlI ACTAGT 1 cut(s) 1139
AjnI CCWGG 2 cut(s) 352, 756
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
Alw21I GWGCWC 2 cut(s) 96, 842
Ama87I CYCGRG 2 cut(s) 463, 883
AoxI GGCC 2 cut(s) 366, 1035
ApeKI GCWGC 8 cut(s) 110, 147, 489, 646, 661, 664, 667, 856
ApoI RAATTY 2 cut(s) 379, 987
Asp700I GAANNNNTTC 2 cut(s) 65, 628
AspLEI GCGC 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 760
AsuC2I CCSGG 3 cut(s) 39, 464, 465
AsuHPI GGTGA 1 cut(s) 275
AsuII TTCGAA 2 cut(s) 505, 562
AvaI CYCGRG 2 cut(s) 463, 883
AvaII GGWCC 1 cut(s) 760
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 3 cut(s) 96, 464, 842
BbrPI CACGTG 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 2 cut(s) 96, 842
BbvCI CCTCAGC 1 cut(s) 267
BbvI GCAGC 8 cut(s) 97, 159, 501, 658, 673, 676, 679, 843
BccI CCATC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 874, 908
BciT130I CCWGG 2 cut(s) 354, 758
BciVI GTATCC 1 cut(s) 122
BcnI CCSGG 3 cut(s) 39, 464, 465
BcuI ACTAGT 1 cut(s) 1139
BfaI CTAG 2 cut(s) 513, 1140
BfmI CTRYAG 1 cut(s) 857
BfoI RGCGCY 1 cut(s) 432
BfuI GTATCC 1 cut(s) 122
BisI GCNGC 9 cut(s) 111, 148, 490, 647, 662, 665, 668, 857, 889
BlsI GCNGC 9 cut(s) 112, 149, 491, 648, 663, 666, 669, 858, 890
Bme1390I CCNGG 5 cut(s) 39, 354, 464, 465, 758
Bme18I GGWCC 1 cut(s) 760
BmeRI GACNNNNNGTC 1 cut(s) 205
BmeT110I CYCGRG 2 cut(s) 463, 883
BmgT120I GGNCC 1 cut(s) 760
BmrFI CCNGG 5 cut(s) 39, 354, 464, 465, 758
BmsI GCATC 1 cut(s) 514
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 267
Bpu14I TTCGAA 2 cut(s) 505, 562
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 3 cut(s) 39, 464, 465
Bsa29I ATCGAT 1 cut(s) 682
BsaAI YACGTR 1 cut(s) 295
BsaJI CCNNGG 3 cut(s) 37, 463, 519
BseBI CCWGG 2 cut(s) 354, 758
BseCI ATCGAT 1 cut(s) 682
BseDI CCNNGG 3 cut(s) 37, 463, 519
BseGI GGATG 3 cut(s) 192, 262, 529
BseMII CTCAG 3 cut(s) 138, 281, 799
BseRI GAGGAG 6 cut(s) 231, 467, 470, 493, 496, 499
BseXI GCAGC 8 cut(s) 97, 159, 501, 658, 673, 676, 679, 843
BseYI CCCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 2 cut(s) 368, 1037
BshVI ATCGAT 1 cut(s) 682
BsiHKAI GWGCWC 2 cut(s) 96, 842
BsiHKCI CYCGRG 2 cut(s) 463, 883
BsiSI CCGG 2 cut(s) 39, 464
BsnI GGCC 2 cut(s) 368, 1037
BsoBI CYCGRG 2 cut(s) 463, 883
Bsp119I TTCGAA 2 cut(s) 505, 562
Bsp1286I GDGCHC 3 cut(s) 96, 464, 842
Bsp1407I TGTACA 1 cut(s) 1053
Bsp143I GATC 7 cut(s) 203, 679, 683, 709, 844, 930, 935
BspACI CCGC 2 cut(s) 357, 888
BspANI GGCC 2 cut(s) 368, 1037
BspCNI CTCAG 3 cut(s) 137, 280, 798
BspDI ATCGAT 1 cut(s) 682
BspHI TCATGA 2 cut(s) 712, 1134
BspMAI CTGCAG 1 cut(s) 861
BspT104I TTCGAA 2 cut(s) 505, 562
BsrBI CCGCTC 1 cut(s) 888
BsrGI TGTACA 1 cut(s) 1053
BssECI CCNNGG 3 cut(s) 37, 463, 519
BssMI GATC 7 cut(s) 203, 679, 683, 709, 844, 930, 935
BssNAI GTATAC 1 cut(s) 1084
Bst1107I GTATAC 1 cut(s) 1084
Bst2UI CCWGG 2 cut(s) 354, 758
Bst4CI ACNGT 2 cut(s) 438, 1117
BstAUI TGTACA 1 cut(s) 1053
BstBAI YACGTR 1 cut(s) 295
BstBI TTCGAA 2 cut(s) 505, 562
BstC8I GCNNGC 4 cut(s) 92, 366, 370, 893
BstDEI CTNAG 3 cut(s) 124, 267, 785
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 3 cut(s) 192, 262, 529
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 7 cut(s) 206, 682, 686, 712, 847, 933, 938
BstMBI GATC 7 cut(s) 203, 679, 683, 709, 844, 930, 935
BstMWI GCNNNNNNNGC 4 cut(s) 228, 354, 667, 781
BstNI CCWGG 2 cut(s) 354, 758
BstNSI RCATGY 2 cut(s) 241, 256
BstSCI CCNGG 5 cut(s) 37, 352, 462, 463, 756
BstSFI CTRYAG 1 cut(s) 857
BstV1I GCAGC 8 cut(s) 97, 159, 501, 658, 673, 676, 679, 843
BstV2I GAAGAC 1 cut(s) 204
BstZ17I GTATAC 1 cut(s) 1084
Bsu15I ATCGAT 1 cut(s) 682
BsuI GTATCC 1 cut(s) 122
BsuRI GGCC 2 cut(s) 368, 1037
BsuTUI ATCGAT 1 cut(s) 682
BtgI CCRYGG 1 cut(s) 519
BtgZI GCGATG 1 cut(s) 314
BtsCI GGATG 3 cut(s) 192, 262, 529
BtsIMutI CAGTG 2 cut(s) 405, 793
Cac8I GCNNGC 4 cut(s) 92, 366, 370, 893
CciI TCATGA 2 cut(s) 712, 1134
CfoI GCGC 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 760
Cfr9I CCCGGG 1 cut(s) 463
ClaI ATCGAT 1 cut(s) 682
Csp6I GTAC 5 cut(s) 15, 118, 334, 901, 1054
CviAII CATG 8 cut(s) 7, 210, 238, 253, 442, 584, 713, 1135
CviQI GTAC 5 cut(s) 15, 118, 334, 901, 1054
DdeI CTNAG 3 cut(s) 124, 267, 785
DpnI GATC 7 cut(s) 205, 681, 685, 711, 846, 932, 937
DpnII GATC 7 cut(s) 203, 679, 683, 709, 844, 930, 935
DriI GACNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 366
Eam1105I GACNNNNNGTC 1 cut(s) 205
Ecl136II GAGCTC 2 cut(s) 94, 840
Eco24I GRGCYC 3 cut(s) 96, 464, 842
Eco47I GGWCC 1 cut(s) 760
Eco53kI GAGCTC 2 cut(s) 94, 840
Eco72I CACGTG 1 cut(s) 295
Eco88I CYCGRG 2 cut(s) 463, 883
EcoICRI GAGCTC 2 cut(s) 94, 840
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 352, 756
EcoT22I ATGCAT 1 cut(s) 964
EcoT38I GRGCYC 3 cut(s) 96, 464, 842
FaeI CATG 8 cut(s) 10, 213, 241, 256, 445, 587, 716, 1138
FatI CATG 8 cut(s) 6, 209, 237, 252, 441, 583, 712, 1134
FauI CCCGC 1 cut(s) 350
FauNDI CATATG 1 cut(s) 964
FblI GTMKAC 2 cut(s) 918, 1083
Fnu4HI GCNGC 9 cut(s) 111, 148, 490, 647, 662, 665, 668, 857, 889
FokI GGATG 3 cut(s) 199, 249, 536
FriOI GRGCYC 3 cut(s) 96, 464, 842
Fsp4HI GCNGC 9 cut(s) 111, 148, 490, 647, 662, 665, 668, 857, 889
FspBI CTAG 2 cut(s) 513, 1140
GlaI GCGC 1 cut(s) 430
GluI GCNGC 9 cut(s) 111, 148, 490, 647, 662, 665, 668, 857, 889
GsaI CCCAGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 432
HaeIII GGCC 2 cut(s) 368, 1037
HapII CCGG 2 cut(s) 39, 464
HhaI GCGC 1 cut(s) 431
Hin1II CATG 8 cut(s) 10, 213, 241, 256, 445, 587, 716, 1138
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HinfI GANTC 4 cut(s) 83, 225, 833, 1122
HpaII CCGG 2 cut(s) 39, 464
HphI GGTGA 1 cut(s) 275
Hpy166II GTNNAC 3 cut(s) 901, 919, 1084
Hpy188I TCNGA 6 cut(s) 248, 634, 733, 754, 844, 935
Hpy188III TCNNGA 8 cut(s) 126, 376, 555, 713, 778, 928, 1103, 1135
Hpy8I GTNNAC 3 cut(s) 901, 919, 1084
HpyAV CCTTC 2 cut(s) 468, 812
HpyCH4III ACNGT 2 cut(s) 438, 1117
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 5 cut(s) 113, 587, 824, 859, 962
HpyF10VI GCNNNNNNNGC 4 cut(s) 228, 354, 667, 781
HpyF3I CTNAG 3 cut(s) 124, 267, 785
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 8 cut(s) 10, 213, 241, 256, 445, 587, 716, 1138
HspAI GCGC 1 cut(s) 429
Kzo9I GATC 7 cut(s) 203, 679, 683, 709, 844, 930, 935
LmnI GCTCC 2 cut(s) 41, 486
Lsp1109I GCAGC 8 cut(s) 97, 159, 501, 658, 673, 676, 679, 843
LweI GCATC 1 cut(s) 514
MaeI CTAG 2 cut(s) 513, 1140
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 1 cut(s) 940
MalI GATC 7 cut(s) 205, 681, 685, 711, 846, 932, 937
MbiI CCGCTC 1 cut(s) 888
MboI GATC 7 cut(s) 203, 679, 683, 709, 844, 930, 935
MboII GAAGA 4 cut(s) 209, 421, 424, 824
MhlI GDGCHC 3 cut(s) 96, 464, 842
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 8 cut(s) 62, 379, 725, 862, 948, 955, 981, 987
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 777
Mox20I TGGCCA 1 cut(s) 368
Mph1103I ATGCAT 1 cut(s) 964
MroXI GAANNNNTTC 2 cut(s) 65, 628
MscI TGGCCA 1 cut(s) 368
MseI TTAA 5 cut(s) 984, 1008, 1023, 1070, 1112
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 2 cut(s) 39, 464
MspR9I CCNGG 5 cut(s) 39, 354, 464, 465, 758
MvaI CCWGG 2 cut(s) 354, 758
MwoI GCNNNNNNNGC 4 cut(s) 228, 354, 667, 781
NciI CCSGG 3 cut(s) 39, 464, 465
NdeI CATATG 1 cut(s) 964
NdeII GATC 7 cut(s) 203, 679, 683, 709, 844, 930, 935
NlaIII CATG 8 cut(s) 10, 213, 241, 256, 445, 587, 716, 1138
NsiI ATGCAT 1 cut(s) 964
NspI RCATGY 2 cut(s) 241, 256
NspV TTCGAA 2 cut(s) 505, 562
PaeR7I CTCGAG 1 cut(s) 883
PagI TCATGA 2 cut(s) 712, 1134
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdmI GAANNNNTTC 2 cut(s) 65, 628
PfeI GAWTC 3 cut(s) 225, 833, 1122
PkrI GCNGC 9 cut(s) 112, 149, 491, 648, 663, 666, 669, 858, 890
PleI GAGTC 1 cut(s) 77
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
PpsI GAGTC 1 cut(s) 77
Ppu21I YACGTR 1 cut(s) 295
Psp124BI GAGCTC 2 cut(s) 96, 842
Psp6I CCWGG 2 cut(s) 352, 756
PspCI CACGTG 1 cut(s) 295
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 352, 756
PspPI GGNCC 1 cut(s) 760
PspXI VCTCGAGB 1 cut(s) 883
PstI CTGCAG 1 cut(s) 861
RsaI GTAC 5 cut(s) 16, 119, 335, 902, 1055
RsaNI GTAC 5 cut(s) 15, 118, 334, 901, 1054
SacI GAGCTC 2 cut(s) 96, 842
SaqAI TTAA 5 cut(s) 984, 1008, 1023, 1070, 1112
SatI GCNGC 9 cut(s) 111, 148, 490, 647, 662, 665, 668, 857, 889
Sau3AI GATC 7 cut(s) 203, 679, 683, 709, 844, 930, 935
Sau96I GGNCC 1 cut(s) 760
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 5 cut(s) 39, 354, 464, 465, 758
SduI GDGCHC 3 cut(s) 96, 464, 842
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 857
Sfr274I CTCGAG 1 cut(s) 883
SfuI TTCGAA 2 cut(s) 505, 562
SinI GGWCC 1 cut(s) 760
SlaI CTCGAG 1 cut(s) 883
SmaI CCCGGG 1 cut(s) 465
SmlI CTYRAG 2 cut(s) 776, 883
SmoI CTYRAG 2 cut(s) 776, 883
SpeI ACTAGT 1 cut(s) 1139
Sse9I AATT 8 cut(s) 62, 379, 725, 862, 948, 955, 981, 987
SsiI CCGC 2 cut(s) 357, 888
SspMI CTAG 2 cut(s) 513, 1140
SstI GAGCTC 2 cut(s) 96, 842
StyD4I CCNGG 5 cut(s) 37, 352, 462, 463, 756
TaaI ACNGT 2 cut(s) 438, 1117
TaiI ACGT 1 cut(s) 297
TasI AATT 8 cut(s) 62, 379, 725, 862, 948, 955, 981, 987
TatI WGTACW 3 cut(s) 14, 333, 1053
TauI GCSGC 1 cut(s) 891
TfiI GAWTC 3 cut(s) 225, 833, 1122
Tru1I TTAA 5 cut(s) 984, 1008, 1023, 1070, 1112
Tru9I TTAA 5 cut(s) 984, 1008, 1023, 1070, 1112
TscAI CASTG 2 cut(s) 405, 793
TseI GCWGC 8 cut(s) 110, 147, 489, 646, 661, 664, 667, 856
TspDTI ATGAA 6 cut(s) 150, 206, 248, 458, 831, 1123
TspGWI ACGGA 2 cut(s) 523, 536
TspMI CCCGGG 1 cut(s) 463
TspRI CASTG 2 cut(s) 405, 793
VpaK11BI GGWCC 1 cut(s) 760
XapI RAATTY 2 cut(s) 379, 987
XceI RCATGY 2 cut(s) 241, 256
XhoI CTCGAG 1 cut(s) 883
XmaI CCCGGG 1 cut(s) 463
XmiI GTMKAC 2 cut(s) 918, 1083
XmnI GAANNNNTTC 2 cut(s) 65, 628
XspI CTAG 2 cut(s) 513, 1140
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.