Rh4DG362100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012045
Physical Location & Seq
Forward (+)
58399982 .. 58401118
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG362100.1

Sequence Viewer

Length: 981 bp
ATGTCATCCATTGCAGGACCTGCAGACCCGGGTGAGAAGGTACACAAGAAATCCTTCCACCGGAGGAACGACTCCGGCGAGCTGGACGTGTTCGAAGCTGCACGGTATTTCTCTGGCTACAACGAAGCTCCGACGATGATGATGAAAGAAGATAGATCGTCATGGAGAGGAGGCAGAATTAGTCTTGACATGCCAATCCGAAACGTGCTTGATCAACAGCTTCATCATCATCATCATCACCATGCGGTGGATCAGAAGCAGAATATGAAAGAAAAGATCAAATACAAGCAGCCAAGCTCTCCAGGTGGGAGACTAGCTAGCTTCTTGAATTCACTTTTCAATCAATCAGGTACCAAGAAGAAGAAATCAAAATCCAGTACCACGCAGTCTATGAAAGACGAGGAGGAGAGTCCAGGTGGGAGGAGAAGAAGAAGAAGCAGCATTAGCCATTTTCGAAGCTCGAGTACTACTACCACCACCGATGCCAAGTCCATATACTCATCTTCAAGCTCTGGCTTCAGAACACCTCCTCCTTACGGTGCACAAATCGCAACGGCCAAGAGCTACAAAGATTGGAGAAGTTACTCAGATCACAAGCCGCAGCAGCAAGTGGTGTCCAATTTGTCCAAGCATAGTGTTCTGTCACATGCAAAATCTACACTCTTGGATGAGAAAGTTTCATCAAACAGAGAGCTAAACTGGTTGGACGAGAAATTCAAATACAACAACCGGCTGTCGTTGTCGGAGAAGTACAAGAATTCAATAGATCAGGATCACAACAAGGGTTTGTTGAAGAGGCTGAGTGAGGTGGTTGATGATGATGATGATGATGGTGCAGAAAGTGATTCGAGCTCCGATCTTTTCGAGTTGCAGAACTATGACTTGGGGGGCTACTACTCAAGCGGTTTGCCTGTGTATGAAACTACCAACGTGGATAGCATCAAGAATATCAGAGGAACCCCGATTGCAAATGCCTCATAG

Protein Analysis

326

Amino Acids

36.76

Weight (kDa)

9.22

Isoelectric Point (pI)

57.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69160
fragaria_vesca FvH4_4g29630
malus_domestica MD13G1059400.v1.1 MD16G1058700.v1.1
prunus_persica Prupe.1G290400_v2.0.a1
pyrus_communis pycom13g05290 pycom16g05130
rosa_chinensis RchiOBHm_Chr4g0437861
rosa_laevigata RLG00000006406
rosa_multiflora Rmu_co8467433.1_g000001 Rmu_sc0008907.1_g000004
rosa_roxburghii Rroxscaffold_5G00379040
rosa_rugosa Rorug04G0304800
rosa_samantha Rh4AG356800 Rh4BG367600 Rh4CG381600 Rh4DG362100
rosa_wichuraiana Rw0G011590 Rw0G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 28
Acc65I GGTACC 1 cut(s) 350
AccB1I GGYRCC 1 cut(s) 350
AccB7I CCANNNNNTGG 1 cut(s) 247
AciI CCGC 3 cut(s) 245, 599, 903
AclWI GGATC 2 cut(s) 258, 780
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 3 cut(s) 328, 713, 757
AcuI CTGAAG 1 cut(s) 502
AfaI GTAC 5 cut(s) 42, 352, 379, 466, 752
AfiI CCNNNNNNNGG 3 cut(s) 60, 247, 536
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 6 cut(s) 328, 340, 507, 718, 762, 793
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 2 cut(s) 301, 412
AjuI GAANNNNNNNTTGG 2 cut(s) 866, 898
AloI GAACNNNNNNTCC 2 cut(s) 514, 546
Alw21I GWGCWC 2 cut(s) 544, 854
Alw26I GTCTC 1 cut(s) 304
Alw44I GTGCAC 1 cut(s) 540
AlwI GGATC 2 cut(s) 258, 780
AlwNI CAGNNNCTG 1 cut(s) 20
Ama87I CYCGRG 2 cut(s) 28, 460
AoxI GGCC 1 cut(s) 555
ApaLI GTGCAC 1 cut(s) 540
ApeKI GCWGC 5 cut(s) 98, 289, 438, 601, 604
ApoI RAATTY 3 cut(s) 328, 713, 757
Asp700I GAANNNNTTC 1 cut(s) 53
Asp718I GGTACC 1 cut(s) 350
AspS9I GGNCC 1 cut(s) 17
AsuC2I CCSGG 2 cut(s) 29, 30
AsuHPI GGTGA 2 cut(s) 44, 230
AsuII TTCGAA 2 cut(s) 93, 454
AsuNHI GCTAGC 1 cut(s) 317
AvaI CYCGRG 2 cut(s) 28, 460
AvaII GGWCC 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 544
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 854
BarI GAAGNNNNNNTAC 2 cut(s) 448, 480
Bbv12I GWGCWC 2 cut(s) 544, 854
BbvI GCAGC 5 cut(s) 85, 301, 450, 613, 616
BccI CCATC 1 cut(s) 824
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 2 cut(s) 303, 414
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 2 cut(s) 29, 30
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 2 cut(s) 314, 318
BfmI CTRYAG 1 cut(s) 21
BfuAI ACCTGC 1 cut(s) 28
BisI GCNGC 6 cut(s) 99, 290, 439, 599, 602, 605
BlsI GCNGC 6 cut(s) 100, 291, 440, 600, 603, 606
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 4 cut(s) 29, 30, 303, 414
Bme18I GGWCC 1 cut(s) 17
BmeT110I CYCGRG 2 cut(s) 28, 460
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 352, 958
BmrFI CCNGG 4 cut(s) 29, 30, 303, 414
BmsI GCATC 2 cut(s) 472, 948
BmtI GCTAGC 1 cut(s) 321
BpmI CTGGAG 1 cut(s) 285
Bpu14I TTCGAA 2 cut(s) 93, 454
BpuEI CTTGAG 1 cut(s) 883
BpuMI CCSGG 2 cut(s) 29, 30
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 1 cut(s) 28
BsaWI WCCGGW 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 247, 536
Bse118I RCCGGY 1 cut(s) 729
Bse1I ACTGG 2 cut(s) 375, 704
Bse3DI GCAATG 1 cut(s) 9
Bse8I GATNNNNATC 1 cut(s) 771
BseBI CCWGG 2 cut(s) 303, 414
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 2 cut(s) 5, 673
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 3 cut(s) 60, 247, 536
BseMI GCAATG 1 cut(s) 9
BseMII CTCAG 2 cut(s) 600, 791
BseNI ACTGG 2 cut(s) 375, 704
BseRI GAGGAG 5 cut(s) 183, 416, 419, 436, 519
BseSI GKGCMC 1 cut(s) 544
BseXI GCAGC 5 cut(s) 85, 301, 450, 613, 616
BsgI GTGCAG 2 cut(s) 84, 855
BshFI GGCC 1 cut(s) 557
BshNI GGYRCC 1 cut(s) 350
BsiHKAI GWGCWC 2 cut(s) 544, 854
BsiHKCI CYCGRG 2 cut(s) 28, 460
BsiSI CCGG 4 cut(s) 29, 61, 75, 730
BslI CCNNNNNNNGG 3 cut(s) 60, 247, 536
BsmAI GTCTC 1 cut(s) 304
BsnI GGCC 1 cut(s) 557
BsoBI CYCGRG 2 cut(s) 28, 460
Bsp119I TTCGAA 2 cut(s) 93, 454
Bsp1286I GDGCHC 2 cut(s) 544, 854
Bsp143I GATC 8 cut(s) 155, 211, 250, 276, 589, 766, 772, 856
BspACI CCGC 3 cut(s) 245, 599, 903
BspANI GGCC 1 cut(s) 557
BspCNI CTCAG 2 cut(s) 599, 792
BspLI GGNNCC 2 cut(s) 352, 958
BspMAI CTGCAG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 28
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 2 cut(s) 258, 780
BspT104I TTCGAA 2 cut(s) 93, 454
BspT107I GGYRCC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 9
BsrFI RCCGGY 1 cut(s) 729
BsrI ACTGG 2 cut(s) 375, 704
BssAI RCCGGY 1 cut(s) 729
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 8 cut(s) 155, 211, 250, 276, 589, 766, 772, 856
Bst2UI CCWGG 2 cut(s) 303, 414
Bst4CI ACNGT 2 cut(s) 105, 539
Bst6I CTCTTC 1 cut(s) 788
BstAPI GCANNNNNTGC 1 cut(s) 20
BstBI TTCGAA 2 cut(s) 93, 454
BstC8I GCNNGC 2 cut(s) 80, 319
BstDEI CTNAG 2 cut(s) 586, 800
BstF5I GGATG 2 cut(s) 5, 673
BstKTI GATC 8 cut(s) 158, 214, 253, 279, 592, 769, 775, 859
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 8 cut(s) 155, 211, 250, 276, 589, 766, 772, 856
BstMWI GCNNNNNNNGC 4 cut(s) 20, 444, 548, 604
BstNI CCWGG 2 cut(s) 303, 414
BstNSI RCATGY 2 cut(s) 193, 650
BstSCI CCNGG 4 cut(s) 27, 28, 301, 412
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 544
BstV1I GCAGC 5 cut(s) 85, 301, 450, 613, 616
BsuRI GGCC 1 cut(s) 557
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 2 cut(s) 5, 673
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 2 cut(s) 80, 319
CaiI CAGNNNCTG 1 cut(s) 20
Cfr10I RCCGGY 1 cut(s) 729
Cfr13I GGNCC 1 cut(s) 17
Cfr9I CCCGGG 1 cut(s) 28
Csp6I GTAC 5 cut(s) 41, 351, 378, 465, 751
CviAII CATG 4 cut(s) 162, 190, 242, 647
CviQI GTAC 5 cut(s) 41, 351, 378, 465, 751
DdeI CTNAG 2 cut(s) 586, 800
DpnI GATC 8 cut(s) 157, 213, 252, 278, 591, 768, 774, 858
DpnII GATC 8 cut(s) 155, 211, 250, 276, 589, 766, 772, 856
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 1 cut(s) 788
EarI CTCTTC 1 cut(s) 788
Ecl136II GAGCTC 1 cut(s) 852
Eco24I GRGCYC 1 cut(s) 854
Eco47I GGWCC 1 cut(s) 17
Eco53kI GAGCTC 1 cut(s) 852
Eco57I CTGAAG 1 cut(s) 502
Eco88I CYCGRG 2 cut(s) 28, 460
EcoICRI GAGCTC 1 cut(s) 852
EcoO109I RGGNCCY 1 cut(s) 17
EcoRI GAATTC 2 cut(s) 328, 757
EcoRII CCWGG 2 cut(s) 301, 412
EcoT38I GRGCYC 1 cut(s) 854
FaeI CATG 4 cut(s) 165, 193, 245, 650
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FatI CATG 4 cut(s) 161, 189, 241, 646
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 6 cut(s) 99, 290, 439, 599, 602, 605
FokI GGATG 1 cut(s) 680
FriOI GRGCYC 1 cut(s) 854
Fsp4HI GCNGC 6 cut(s) 99, 290, 439, 599, 602, 605
FspBI CTAG 2 cut(s) 314, 318
GluI GCNGC 6 cut(s) 99, 290, 439, 599, 602, 605
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 1 cut(s) 557
HapII CCGG 4 cut(s) 29, 61, 75, 730
Hin1II CATG 4 cut(s) 165, 193, 245, 650
HinfI GANTC 3 cut(s) 71, 409, 845
HpaII CCGG 4 cut(s) 29, 61, 75, 730
HphI GGTGA 2 cut(s) 44, 230
Hpy166II GTNNAC 2 cut(s) 43, 542
Hpy188I TCNGA 8 cut(s) 132, 200, 255, 521, 589, 745, 856, 953
Hpy188III TCNNGA 4 cut(s) 185, 325, 770, 943
Hpy8I GTNNAC 2 cut(s) 43, 542
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 2 cut(s) 31, 64
HpyCH4III ACNGT 2 cut(s) 105, 539
HpyCH4IV ACGT 3 cut(s) 87, 204, 930
HpyCH4V TGCA 8 cut(s) 14, 23, 101, 542, 650, 836, 871, 968
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 444, 548, 604
HpyF3I CTNAG 2 cut(s) 586, 800
HpySE526I ACGT 3 cut(s) 87, 204, 930
Hsp92II CATG 4 cut(s) 165, 193, 245, 650
KpnI GGTACC 1 cut(s) 354
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 8 cut(s) 155, 211, 250, 276, 589, 766, 772, 856
LmnI GCTCC 2 cut(s) 133, 857
Lsp1109I GCAGC 5 cut(s) 85, 301, 450, 613, 616
LweI GCATC 2 cut(s) 472, 948
MaeI CTAG 2 cut(s) 314, 318
MaeII ACGT 3 cut(s) 87, 204, 930
MaeIII GTNAC 2 cut(s) 581, 642
MalI GATC 8 cut(s) 157, 213, 252, 278, 591, 768, 774, 858
MboI GATC 8 cut(s) 155, 211, 250, 276, 589, 766, 772, 856
MboII GAAGA 8 cut(s) 161, 370, 373, 438, 441, 444, 495, 805
MhlI GDGCHC 2 cut(s) 544, 854
MluCI AATT 5 cut(s) 177, 328, 619, 713, 757
MlyI GAGTC 2 cut(s) 65, 418
MmeI TCCRAC 3 cut(s) 155, 684, 723
MroXI GAANNNNTTC 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 240
MspI CCGG 4 cut(s) 29, 61, 75, 730
MspR9I CCNGG 4 cut(s) 29, 30, 303, 414
MvaI CCWGG 2 cut(s) 303, 414
MwoI GCNNNNNNNGC 4 cut(s) 20, 444, 548, 604
NciI CCSGG 2 cut(s) 29, 30
NdeII GATC 8 cut(s) 155, 211, 250, 276, 589, 766, 772, 856
NheI GCTAGC 1 cut(s) 317
NlaIII CATG 4 cut(s) 165, 193, 245, 650
NlaIV GGNNCC 2 cut(s) 352, 958
NmuCI GTSAC 1 cut(s) 642
NspI RCATGY 2 cut(s) 193, 650
NspV TTCGAA 2 cut(s) 93, 454
PaeR7I CTCGAG 1 cut(s) 460
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 845
PflMI CCANNNNNTGG 1 cut(s) 247
PkrI GCNGC 6 cut(s) 100, 291, 440, 600, 603, 606
PleI GAGTC 2 cut(s) 65, 417
PpsI GAGTC 2 cut(s) 65, 417
PpuMI RGGWCCY 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 854
Psp5II RGGWCCY 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 301, 412
PspGI CCWGG 2 cut(s) 301, 412
PspN4I GGNNCC 2 cut(s) 352, 958
PspPI GGNCC 1 cut(s) 17
PspPPI RGGWCCY 1 cut(s) 17
PspXI VCTCGAGB 1 cut(s) 460
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 20
RsaI GTAC 5 cut(s) 42, 352, 379, 466, 752
RsaNI GTAC 5 cut(s) 41, 351, 378, 465, 751
RseI CAYNNNNRTG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 854
SatI GCNGC 6 cut(s) 99, 290, 439, 599, 602, 605
Sau3AI GATC 8 cut(s) 155, 211, 250, 276, 589, 766, 772, 856
Sau96I GGNCC 1 cut(s) 17
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 2 cut(s) 65, 418
ScrFI CCNGG 4 cut(s) 29, 30, 303, 414
SduI GDGCHC 2 cut(s) 544, 854
SfaNI GCATC 2 cut(s) 472, 948
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 460
SfuI TTCGAA 2 cut(s) 93, 454
SinI GGWCC 1 cut(s) 17
SlaI CTCGAG 1 cut(s) 460
SmaI CCCGGG 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 2 cut(s) 460, 898
SmoI CTYRAG 2 cut(s) 460, 898
Sse9I AATT 5 cut(s) 177, 328, 619, 713, 757
SsiI CCGC 3 cut(s) 245, 599, 903
SspMI CTAG 2 cut(s) 314, 318
SstI GAGCTC 1 cut(s) 854
StyD4I CCNGG 4 cut(s) 27, 28, 301, 412
TaaI ACNGT 2 cut(s) 105, 539
TaiI ACGT 3 cut(s) 90, 207, 933
TaqI TCGA 5 cut(s) 93, 454, 461, 848, 864
TasI AATT 5 cut(s) 177, 328, 619, 713, 757
TatI WGTACW 2 cut(s) 464, 750
TauI GCSGC 1 cut(s) 601
TfiI GAWTC 1 cut(s) 845
TseFI GTSAC 1 cut(s) 642
TseI GCWGC 5 cut(s) 98, 289, 438, 601, 604
Tsp45I GTSAC 1 cut(s) 642
TspDTI ATGAA 6 cut(s) 158, 212, 281, 407, 669, 933
TspMI CCCGGG 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 247
VneI GTGCAC 1 cut(s) 540
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 3 cut(s) 328, 713, 757
XceI RCATGY 2 cut(s) 193, 650
XhoI CTCGAG 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 28
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 2 cut(s) 314, 318
ZrmI AGTACT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.