Rw0G016090

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0012045
Physical Location & Seq
Forward (+)
32878 .. 33861
984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016090.1

Sequence Viewer

Length: 984 bp
ATGTCATCCATTGCAGGACTTGCAGACCCGGGTGAGAAGGTACACAAGAAATCCTTCCACCGGAGGAACGATTCCGGCGAGCTGGACGTGTTCGAAGCTGCACGGTATTTCTCTGGCTACAACGAAGCTCCGACGATAATGATGAAAGAAGATAGATCGTCATGGAGAGGAGGCAGAATTAGTCTTGACATGCCAATCCGAAACGTGCTTGATCAACAGCTTCATCATCATCATCATCATCATCACCATGCGGTGGATCAAAAGCAGAATATGAAAGAAAAGATCAAATACAAGCAGCCAAGCTCTCCAGGTGGGAGACTAGCTAGCTTCTTGAATTCACTTTTCAATCAATCAGGTTCTAAGAAGAAGAAATCAAAATCCAGTACCACGCAGTCTATGAAAGACGAGGAGGAGAGTCCAGGTGGGAGGAGAAGAAGAAGAAGCAGCATTAGCCATTTTCGAAGCTCGAGTACTACTACCACCACCGATGCCAAGTCCATATACTCATCTTCAAGCTCTGGCTTCAGAACACCTCCTCCTTACGGTGCACAAATAGCAACGGCCAAGAGCTACAAAGATTGGAGAAGTTACTCAGATCACAAGCCGCAGCAGCAAGTGGTGTCCAATTTGTCCAAGCATAGTGTTCTGTCACATGCAAAATCTACACTCTTGGATGAGAAAGTTTCATCAAACAGAGAGCTAAACTGGTTGGACGAGAAATTCAAATACAACAACCGGCTGTCGTTGTCGGAGAAGTACAAGAATTCAATAGATCAGGATCACAACAAGGGATTGTTGAAGAGGCTGAGTGAGGTGGTTGATGATGATGATGATGGTGCAGAAAGTGATTCGAGCTCCGATCTTTTCGAGTTGCAGAACTATGACTTGGGGGGCTACTACTCAAGCGGTTTGCCTGTTTATGAAACTACCAACGTGGATAGCATCAAGAATATCAGAGGAACCCCGATTGCAAATGCCTCATAG

Protein Analysis

327

Amino Acids

36.9

Weight (kDa)

9.3

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69160
fragaria_vesca FvH4_4g29630
malus_domestica MD13G1059400.v1.1 MD16G1058700.v1.1
prunus_persica Prupe.1G290400_v2.0.a1
pyrus_communis pycom13g05290 pycom16g05130
rosa_chinensis RchiOBHm_Chr4g0437861
rosa_laevigata RLG00000006406
rosa_multiflora Rmu_co8467433.1_g000001 Rmu_sc0008907.1_g000004
rosa_roxburghii Rroxscaffold_5G00379040
rosa_rugosa Rorug04G0304800
rosa_samantha Rh4AG356800 Rh4BG367600 Rh4CG381600 Rh4DG362100
rosa_wichuraiana Rw0G011590 Rw0G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 253
AciI CCGC 3 cut(s) 251, 605, 906
AclWI GGATC 2 cut(s) 264, 786
AcoI YGGCCR 1 cut(s) 561
AcsI RAATTY 3 cut(s) 334, 719, 763
AcuI CTGAAG 1 cut(s) 508
AfaI GTAC 4 cut(s) 42, 385, 472, 758
AfiI CCNNNNNNNGG 3 cut(s) 60, 253, 542
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 6 cut(s) 334, 346, 513, 724, 768, 799
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 2 cut(s) 307, 418
AjuI GAANNNNNNNTTGG 2 cut(s) 869, 901
AloI GAACNNNNNNTCC 2 cut(s) 520, 552
Alw21I GWGCWC 2 cut(s) 550, 857
Alw26I GTCTC 1 cut(s) 310
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 2 cut(s) 264, 786
Ama87I CYCGRG 2 cut(s) 28, 466
AoxI GGCC 1 cut(s) 561
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 5 cut(s) 98, 295, 444, 607, 610
ApoI RAATTY 3 cut(s) 334, 719, 763
Asp700I GAANNNNTTC 1 cut(s) 53
AsuC2I CCSGG 2 cut(s) 29, 30
AsuHPI GGTGA 2 cut(s) 44, 236
AsuII TTCGAA 2 cut(s) 93, 460
AsuNHI GCTAGC 1 cut(s) 323
AvaI CYCGRG 2 cut(s) 28, 466
BaeGI GKGCMC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 857
BarI GAAGNNNNNNTAC 2 cut(s) 454, 486
Bbv12I GWGCWC 2 cut(s) 550, 857
BbvI GCAGC 5 cut(s) 85, 307, 456, 619, 622
BccI CCATC 1 cut(s) 827
BceAI ACGGC 1 cut(s) 576
BciT130I CCWGG 2 cut(s) 309, 420
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 2 cut(s) 29, 30
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 2 cut(s) 320, 324
BisI GCNGC 6 cut(s) 99, 296, 445, 605, 608, 611
BlsI GCNGC 6 cut(s) 100, 297, 446, 606, 609, 612
BmcAI AGTACT 1 cut(s) 472
Bme1390I CCNGG 4 cut(s) 29, 30, 309, 420
BmeT110I CYCGRG 2 cut(s) 28, 466
BmgBI CACGTC 1 cut(s) 88
BmiI GGNNCC 1 cut(s) 961
BmrFI CCNGG 4 cut(s) 29, 30, 309, 420
BmsI GCATC 2 cut(s) 478, 951
BmtI GCTAGC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 291
Bpu14I TTCGAA 2 cut(s) 93, 460
BpuEI CTTGAG 1 cut(s) 886
BpuMI CCSGG 2 cut(s) 29, 30
BsaBI GATNNNNATC 1 cut(s) 777
BsaJI CCNNGG 1 cut(s) 28
BsaWI WCCGGW 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 520, 550
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 253, 542
Bse118I RCCGGY 1 cut(s) 735
Bse1I ACTGG 2 cut(s) 381, 710
Bse3DI GCAATG 1 cut(s) 9
Bse8I GATNNNNATC 1 cut(s) 777
BseBI CCWGG 2 cut(s) 309, 420
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 2 cut(s) 5, 679
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 3 cut(s) 60, 253, 542
BseMI GCAATG 1 cut(s) 9
BseMII CTCAG 2 cut(s) 606, 797
BseNI ACTGG 2 cut(s) 381, 710
BseRI GAGGAG 5 cut(s) 183, 422, 425, 442, 525
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 5 cut(s) 85, 307, 456, 619, 622
BsgI GTGCAG 2 cut(s) 84, 858
BshFI GGCC 1 cut(s) 563
BsiHKAI GWGCWC 2 cut(s) 550, 857
BsiHKCI CYCGRG 2 cut(s) 28, 466
BsiSI CCGG 4 cut(s) 29, 61, 75, 736
BslI CCNNNNNNNGG 3 cut(s) 60, 253, 542
BsmAI GTCTC 1 cut(s) 310
BsnI GGCC 1 cut(s) 563
BsoBI CYCGRG 2 cut(s) 28, 466
Bsp119I TTCGAA 2 cut(s) 93, 460
Bsp1286I GDGCHC 2 cut(s) 550, 857
Bsp143I GATC 8 cut(s) 155, 211, 256, 282, 595, 772, 778, 859
BspACI CCGC 3 cut(s) 251, 605, 906
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 2 cut(s) 605, 798
BspLI GGNNCC 1 cut(s) 961
BspOI GCTAGC 1 cut(s) 327
BspPI GGATC 2 cut(s) 264, 786
BspT104I TTCGAA 2 cut(s) 93, 460
BsrDI GCAATG 1 cut(s) 9
BsrFI RCCGGY 1 cut(s) 735
BsrI ACTGG 2 cut(s) 381, 710
BssAI RCCGGY 1 cut(s) 735
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 8 cut(s) 155, 211, 256, 282, 595, 772, 778, 859
Bst2UI CCWGG 2 cut(s) 309, 420
Bst4CI ACNGT 2 cut(s) 105, 545
Bst6I CTCTTC 1 cut(s) 794
BstAPI GCANNNNNTGC 1 cut(s) 20
BstBI TTCGAA 2 cut(s) 93, 460
BstC8I GCNNGC 2 cut(s) 80, 325
BstDEI CTNAG 3 cut(s) 360, 592, 806
BstF5I GGATG 2 cut(s) 5, 679
BstKTI GATC 8 cut(s) 158, 214, 259, 285, 598, 775, 781, 862
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 8 cut(s) 155, 211, 256, 282, 595, 772, 778, 859
BstMWI GCNNNNNNNGC 4 cut(s) 20, 450, 554, 610
BstNI CCWGG 2 cut(s) 309, 420
BstNSI RCATGY 2 cut(s) 193, 656
BstSCI CCNGG 4 cut(s) 27, 28, 307, 418
BstSLI GKGCMC 1 cut(s) 550
BstV1I GCAGC 5 cut(s) 85, 307, 456, 619, 622
BsuRI GGCC 1 cut(s) 563
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 2 cut(s) 5, 679
Cac8I GCNNGC 2 cut(s) 80, 325
Cfr10I RCCGGY 1 cut(s) 735
Cfr9I CCCGGG 1 cut(s) 28
Csp6I GTAC 4 cut(s) 41, 384, 471, 757
CviAII CATG 4 cut(s) 162, 190, 248, 653
CviQI GTAC 4 cut(s) 41, 384, 471, 757
DdeI CTNAG 3 cut(s) 360, 592, 806
DpnI GATC 8 cut(s) 157, 213, 258, 284, 597, 774, 780, 861
DpnII GATC 8 cut(s) 155, 211, 256, 282, 595, 772, 778, 859
EaeI YGGCCR 1 cut(s) 561
Eam1104I CTCTTC 1 cut(s) 794
EarI CTCTTC 1 cut(s) 794
Ecl136II GAGCTC 1 cut(s) 855
Eco24I GRGCYC 1 cut(s) 857
Eco53kI GAGCTC 1 cut(s) 855
Eco57I CTGAAG 1 cut(s) 508
Eco88I CYCGRG 2 cut(s) 28, 466
EcoICRI GAGCTC 1 cut(s) 855
EcoRI GAATTC 2 cut(s) 334, 763
EcoRII CCWGG 2 cut(s) 307, 418
EcoT38I GRGCYC 1 cut(s) 857
FaeI CATG 4 cut(s) 165, 193, 251, 656
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FatI CATG 4 cut(s) 161, 189, 247, 652
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 6 cut(s) 99, 296, 445, 605, 608, 611
FokI GGATG 1 cut(s) 686
FriOI GRGCYC 1 cut(s) 857
Fsp4HI GCNGC 6 cut(s) 99, 296, 445, 605, 608, 611
FspBI CTAG 2 cut(s) 320, 324
GluI GCNGC 6 cut(s) 99, 296, 445, 605, 608, 611
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 1 cut(s) 563
HapII CCGG 4 cut(s) 29, 61, 75, 736
Hin1II CATG 4 cut(s) 165, 193, 251, 656
HinfI GANTC 3 cut(s) 71, 415, 848
HpaII CCGG 4 cut(s) 29, 61, 75, 736
HphI GGTGA 2 cut(s) 44, 236
Hpy166II GTNNAC 2 cut(s) 43, 548
Hpy188I TCNGA 7 cut(s) 132, 200, 527, 595, 751, 859, 956
Hpy188III TCNNGA 4 cut(s) 185, 331, 776, 946
Hpy8I GTNNAC 2 cut(s) 43, 548
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 2 cut(s) 31, 64
HpyCH4III ACNGT 2 cut(s) 105, 545
HpyCH4IV ACGT 3 cut(s) 87, 204, 933
HpyCH4V TGCA 8 cut(s) 14, 23, 101, 548, 656, 839, 874, 971
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 450, 554, 610
HpyF3I CTNAG 3 cut(s) 360, 592, 806
HpySE526I ACGT 3 cut(s) 87, 204, 933
Hsp92II CATG 4 cut(s) 165, 193, 251, 656
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 8 cut(s) 155, 211, 256, 282, 595, 772, 778, 859
LmnI GCTCC 2 cut(s) 133, 860
Lsp1109I GCAGC 5 cut(s) 85, 307, 456, 619, 622
LweI GCATC 2 cut(s) 478, 951
MaeI CTAG 2 cut(s) 320, 324
MaeII ACGT 3 cut(s) 87, 204, 933
MaeIII GTNAC 2 cut(s) 587, 648
MalI GATC 8 cut(s) 157, 213, 258, 284, 597, 774, 780, 861
MboI GATC 8 cut(s) 155, 211, 256, 282, 595, 772, 778, 859
MboII GAAGA 8 cut(s) 161, 376, 379, 444, 447, 450, 501, 811
MhlI GDGCHC 2 cut(s) 550, 857
MluCI AATT 5 cut(s) 177, 334, 625, 719, 763
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 3 cut(s) 155, 690, 729
MroXI GAANNNNTTC 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 246
MspI CCGG 4 cut(s) 29, 61, 75, 736
MspR9I CCNGG 4 cut(s) 29, 30, 309, 420
MvaI CCWGG 2 cut(s) 309, 420
MwoI GCNNNNNNNGC 4 cut(s) 20, 450, 554, 610
NciI CCSGG 2 cut(s) 29, 30
NdeII GATC 8 cut(s) 155, 211, 256, 282, 595, 772, 778, 859
NheI GCTAGC 1 cut(s) 323
NlaIII CATG 4 cut(s) 165, 193, 251, 656
NlaIV GGNNCC 1 cut(s) 961
NmuCI GTSAC 1 cut(s) 648
NspI RCATGY 2 cut(s) 193, 656
NspV TTCGAA 2 cut(s) 93, 460
PaeR7I CTCGAG 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 2 cut(s) 71, 848
PflMI CCANNNNNTGG 1 cut(s) 253
PkrI GCNGC 6 cut(s) 100, 297, 446, 606, 609, 612
PleI GAGTC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 857
Psp6I CCWGG 2 cut(s) 307, 418
PspGI CCWGG 2 cut(s) 307, 418
PspN4I GGNNCC 1 cut(s) 961
PspXI VCTCGAGB 1 cut(s) 466
RsaI GTAC 4 cut(s) 42, 385, 472, 758
RsaNI GTAC 4 cut(s) 41, 384, 471, 757
RseI CAYNNNNRTG 1 cut(s) 246
SacI GAGCTC 1 cut(s) 857
SatI GCNGC 6 cut(s) 99, 296, 445, 605, 608, 611
Sau3AI GATC 8 cut(s) 155, 211, 256, 282, 595, 772, 778, 859
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 4 cut(s) 29, 30, 309, 420
SduI GDGCHC 2 cut(s) 550, 857
SfaNI GCATC 2 cut(s) 478, 951
Sfr274I CTCGAG 1 cut(s) 466
SfuI TTCGAA 2 cut(s) 93, 460
SlaI CTCGAG 1 cut(s) 466
SmaI CCCGGG 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 2 cut(s) 466, 901
SmoI CTYRAG 2 cut(s) 466, 901
Sse9I AATT 5 cut(s) 177, 334, 625, 719, 763
SsiI CCGC 3 cut(s) 251, 605, 906
SspMI CTAG 2 cut(s) 320, 324
SstI GAGCTC 1 cut(s) 857
StyD4I CCNGG 4 cut(s) 27, 28, 307, 418
TaaI ACNGT 2 cut(s) 105, 545
TaiI ACGT 3 cut(s) 90, 207, 936
TaqI TCGA 5 cut(s) 93, 460, 467, 851, 867
TasI AATT 5 cut(s) 177, 334, 625, 719, 763
TatI WGTACW 2 cut(s) 470, 756
TauI GCSGC 1 cut(s) 607
TfiI GAWTC 2 cut(s) 71, 848
TseFI GTSAC 1 cut(s) 648
TseI GCWGC 5 cut(s) 98, 295, 444, 607, 610
Tsp45I GTSAC 1 cut(s) 648
TspDTI ATGAA 6 cut(s) 158, 212, 287, 413, 675, 936
TspMI CCCGGG 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 253
VneI GTGCAC 1 cut(s) 546
XapI RAATTY 3 cut(s) 334, 719, 763
XceI RCATGY 2 cut(s) 193, 656
XhoI CTCGAG 1 cut(s) 466
XmaI CCCGGG 1 cut(s) 28
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 2 cut(s) 320, 324
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.