MD13G1068300.v1.1

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005229
Physical Location & Seq
Reverse (-)
4689097 .. 4693664
4568 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1068300.v1.1.491

Sequence Viewer

Length: 2154 bp
ATGCTGGTTTCCGCCCTGGTGACGTCACTCGCAATCAACTCCGGCCTCTGCGTCCTCTTCTTCACGCTCTACTCGATACTCCGGAAGCAGCCGAGCAACTATGAGGTCTACATGCCACGGCTGCTCGCCGAGGGGGAGTTCGACTCTTCGAGTCGTTTCAACCTCGAGCGGCTGATTCCGTCGCCGCACTGGCTCAAGCGGGCCTGGCAGCTCACGGAGGATGACTTGCTGTCGTCCTCCGGCCTCGACGCCGTCGTTTTCATGCGGCTCATTAATTTTAGTCTCAGAGTGTTTCTGTTCGCCGGAGTTATCGGGGTCTTCGTCCTGCTTCCGGTCAACTGCTACGGGAACCAGCTCGAGTATGTTGACTTCGCCGATCTGTCGAATAACTCTCTCGACGTGTTTACGATTTCGAATATGAACGATGGCTCGAGCAAGTTATGGATTCATTTTGCTGCTGTGTACCTTGTTACCATCTTTGTCTGCTGTCTACTTTATTATGAATATAGATATATTTCCCAAAGACGGCTTGATTATTTTCAGTCATCCAAACCTCAACCACATCAGTTCACGATCTTAGTTCGCAGCATTCCTGTTCCTTATGGGAGCAGCGTCAGCGAAAGCGTTGACAGCTTCTTTAGAGAGTATCACCCTTCAACATATCTGTCGCATATTGTTGTTCGTCGAACAAACAATCTTGAAAAACTCCTTAAAGATGCGGAAAAACTCTATACCAGGCTCATTCACTTGCAATCAGATCCCAACCAGCAAAAGTATAAACGATCTTGTTGTTTTGGATTGCTTGGACGCAAGGTTAATCTTGTGGATCACTATGAAAAGAAGCTTGAAGGCGTAGAGGAGATTGTGAGATCGGAGCAATCAGAGGCTTCATTAGGAGTAGAAGAAGTTCGAGCTGCTTTTGTTTCCTTCAAGTCTCGGTATGGTGCTGCAGTTACTCACCGCTTGAAACAATCAAACAATCCTACCAAGTGGGTCACAGAGGAAGCTCCTGAACCTCATGATGTTTATTGGCCGTTCTTCTCGTCATCTTTCATCCGAAGATGGATATCTAAGCTTGTGGTTATAATTTTATGCATAGTCCTAACAATCTTGTTTCTTATTCCTGTTCTAATTGTCCAAGGTCTTACTAACCTAAACCAGCTGGAAGTTTGGCTTCCCTTTCTCGAAAGCGTTCTAACCATAACATTTGTTAGTCAGGTCGTCACAGGATACCTTCCCAGTCTTATTCTTCTATTATTTCTGAAAACGGTGCCCCCCGTCATGGAGTTCTTTTCATCCATTCAAGGATATGTTTCTCTCAGTGATATACAAAAGAGTGCGTGTACAAAAGTACTTTGGTTCACAATATGGAACATTTTTTTTGTTACTGTATTTTCCGGATCAGTTTTCTCTGCGGTTTCTATCTTTCTTGACCCCAAGAATATTCCTTCAAAGTTAGGCGTTTCTGTTCCAGCACAGTCGTCTTTTTTCATTGCGTATGTAGTCACTGCAGGATGGACGAGTACTTCATCAGAACTCTTTCGCATAATTCCTCTGATATGGAGTCTATTAAAAAGACCTTTTACTGACAGTAAAGATGATGAATTTGAAGTTCCAGCAATTAGTTACCATAGTCACATGCCTAGAGTTCTTTTCTTTGTACTTCTCGGTATCACATACTTCTTCCTTGCTCCACTGATTCTTCCCTTCCTCTTGGTGTACCTCTGTCTCGGATTCATCATCTATCGCAACCAGTTCATGAACGTATATGCTCCTAGGTACGAAACTGCTGGCAAGTTTTGGCCGATCGTGCATACTTCAATGATATTCTCTCTGGTACTTATGCACGCTATTGCAGTTGGAATCTTTACAATCAAGAAGGTCGATCTAGCATCTACATTAACTTTTCCTCTTCCTGTAATCACACTTCTTTTCAATGAGTACTGCCGGAAGCGCTTCCTTCCAAATTTCGTTGCTTACCCTGCCGAGAGTTTGATACGAAAGGACAGGCAAGACGAGAACGATCCTTCAATGCCTGAATTTTTCGATAACTTGGTCACAGTCTATCAGGACCCCGATTTGATGCCTATCAACTGTTCTGGGAGCAGTGACAGGCTCAGCAGTCCCCTTTTATCTTCTGCTCATCGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

718

Amino Acids

82.06

Weight (kDa)

7.9

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 168 1.7e-38 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 189 - 345 6.8e-37 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 358 - 623 3.7e-75 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 1270
AccBSI CCGCTC 1 cut(s) 169
AccI GTMKAC 2 cut(s) 108, 490
AccIII TCCGGA 2 cut(s) 81, 1397
AciI CCGC 8 cut(s) 12, 169, 185, 199, 265, 719, 961, 1415
AclWI GGATC 4 cut(s) 752, 834, 1408, 2018
AcoI YGGCCR 2 cut(s) 1031, 1802
AcsI RAATTY 3 cut(s) 1604, 1966, 2039
AcyI GRCGYC 2 cut(s) 23, 249
AfaI GTAC 9 cut(s) 464, 1345, 1353, 1525, 1662, 1721, 1781, 1839, 1943
AfeI AGCGCT 1 cut(s) 1955
AfiI CCNNNNNNNGG 3 cut(s) 331, 525, 1282
AflIII ACRYGT 1 cut(s) 399
AhdI GACNNNNNGTC 1 cut(s) 229
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 3 cut(s) 15, 203, 734
AluBI AGCT 8 cut(s) 211, 355, 633, 844, 914, 1007, 1075, 1162
AluI AGCT 8 cut(s) 211, 355, 633, 844, 914, 1007, 1075, 1162
Alw26I GTCTC 3 cut(s) 287, 939, 1733
AlwI GGATC 4 cut(s) 752, 834, 1408, 2018
Ama87I CYCGRG 3 cut(s) 164, 356, 430
Aor13HI TCCGGA 2 cut(s) 81, 1397
Aor51HI AGCGCT 1 cut(s) 1955
AoxI GGCC 5 cut(s) 43, 201, 241, 1031, 1802
ApeKI GCWGC 8 cut(s) 88, 121, 208, 455, 585, 609, 914, 947
ApoI RAATTY 3 cut(s) 1604, 1966, 2039
AseI ATTAAT 1 cut(s) 273
Asp700I GAANNNNTTC 4 cut(s) 906, 1191, 1539, 1955
AspA2I CCTAGG 1 cut(s) 1775
AspLEI GCGC 1 cut(s) 1956
AspS9I GGNCC 2 cut(s) 201, 2071
AsuHPI GGTGA 3 cut(s) 31, 641, 950
AsuII TTCGAA 1 cut(s) 413
AvaI CYCGRG 3 cut(s) 164, 356, 430
AvaII GGWCC 1 cut(s) 2071
AvrII CCTAGG 1 cut(s) 1775
BaeGI GKGCMC 1 cut(s) 1275
BanI GGYRCC 1 cut(s) 1270
BarI GAAGNNNNNNTAC 4 cut(s) 353, 385, 1911, 1943
BbsI GAAGAC 1 cut(s) 310
BbvI GCAGC 8 cut(s) 100, 108, 220, 442, 597, 621, 901, 934
BccI CCATC 4 cut(s) 419, 482, 1056, 1509
BceAI ACGGC 4 cut(s) 134, 236, 542, 1018
BciT130I CCWGG 3 cut(s) 17, 205, 736
BciVI GTATCC 1 cut(s) 1223
BcoDI GTCTC 3 cut(s) 287, 939, 1733
BfaI CTAG 3 cut(s) 1644, 1776, 1889
BfmI CTRYAG 2 cut(s) 948, 1509
BfoI RGCGCY 1 cut(s) 1957
BfuI GTATCC 1 cut(s) 1223
BglI GCCNNNNNGGC 1 cut(s) 190
BlnI CCTAGG 1 cut(s) 1775
BlpI GCTNAGC 1 cut(s) 2117
BmcAI AGTACT 3 cut(s) 1353, 1525, 1943
Bme1390I CCNGG 3 cut(s) 17, 205, 736
Bme18I GGWCC 1 cut(s) 2071
BmeRI GACNNNNNGTC 1 cut(s) 229
BmeT110I CYCGRG 3 cut(s) 164, 356, 430
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 201, 2071
BmiI GGNNCC 3 cut(s) 350, 1272, 2073
BmrFI CCNGG 3 cut(s) 17, 205, 736
BmrI ACTGGG 1 cut(s) 1233
BmsI GCATC 3 cut(s) 706, 1901, 2073
BmuI ACTGGG 1 cut(s) 1233
BpiI GAAGAC 1 cut(s) 310
BplI GAGNNNNNCTC 2 cut(s) 128, 160
Bpu1102I GCTNAGC 1 cut(s) 2117
Bpu14I TTCGAA 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 179
BsaBI GATNNNNATC 2 cut(s) 1066, 2087
BsaHI GRCGYC 2 cut(s) 23, 249
BsaJI CCNNGG 5 cut(s) 15, 116, 129, 1138, 1775
BsaWI WCCGGW 3 cut(s) 81, 331, 1397
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 525, 1282
Bse1I ACTGG 3 cut(s) 194, 1239, 1753
Bse3DI GCAATG 1 cut(s) 1491
Bse8I GATNNNNATC 2 cut(s) 1066, 2087
BseAI TCCGGA 2 cut(s) 81, 1397
BseBI CCWGG 3 cut(s) 17, 205, 736
BseDI CCNNGG 5 cut(s) 15, 116, 129, 1138, 1775
BseGI GGATG 5 cut(s) 226, 545, 1053, 1295, 1520
BseJI GATNNNNATC 2 cut(s) 1066, 2087
BseLI CCNNNNNNNGG 3 cut(s) 331, 525, 1282
BseMI GCAATG 1 cut(s) 1491
BseMII CTCAG 3 cut(s) 298, 1333, 2131
BseNI ACTGG 3 cut(s) 194, 1239, 1753
BseRI GAGGAG 1 cut(s) 872
BseSI GKGCMC 1 cut(s) 1275
BseXI GCAGC 8 cut(s) 100, 108, 220, 442, 597, 621, 901, 934
Bsh1285I CGRYCG 1 cut(s) 1809
BshFI GGCC 5 cut(s) 45, 203, 243, 1033, 1804
BshNI GGYRCC 1 cut(s) 1270
BsiEI CGRYCG 1 cut(s) 1809
BsiHKCI CYCGRG 3 cut(s) 164, 356, 430
BsiSI CCGG 7 cut(s) 42, 82, 240, 303, 332, 1398, 1948
BslFI GGGAC 1 cut(s) 2109
BslI CCNNNNNNNGG 3 cut(s) 331, 525, 1282
BsmAI GTCTC 3 cut(s) 287, 939, 1733
BsmFI GGGAC 1 cut(s) 2109
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 5 cut(s) 45, 203, 243, 1033, 1804
BsoBI CYCGRG 3 cut(s) 164, 356, 430
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 1275
Bsp13I TCCGGA 2 cut(s) 81, 1397
Bsp1407I TGTACA 1 cut(s) 1343
Bsp1720I GCTNAGC 1 cut(s) 2117
BspACI CCGC 8 cut(s) 12, 169, 185, 199, 265, 719, 961, 1415
BspANI GGCC 5 cut(s) 45, 203, 243, 1033, 1804
BspCNI CTCAG 3 cut(s) 297, 1332, 2130
BspEI TCCGGA 2 cut(s) 81, 1397
BspHI TCATGA 2 cut(s) 1018, 1758
BspLI GGNNCC 3 cut(s) 350, 1272, 2073
BspMAI CTGCAG 2 cut(s) 952, 1513
BspPI GGATC 4 cut(s) 752, 834, 1408, 2018
BspT104I TTCGAA 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 1270
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 1491
BsrGI TGTACA 1 cut(s) 1343
BsrI ACTGG 3 cut(s) 194, 1239, 1753
BssECI CCNNGG 5 cut(s) 15, 116, 129, 1138, 1775
BssNI GRCGYC 2 cut(s) 23, 249
BssT1I CCWWGG 2 cut(s) 1138, 1775
Bst2UI CCWGG 3 cut(s) 17, 205, 736
Bst4CI ACNGT 6 cut(s) 1270, 1390, 1479, 1592, 2062, 2096
Bst6I CTCTTC 3 cut(s) 62, 151, 1917
BstACI GRCGYC 2 cut(s) 23, 249
BstAUI TGTACA 1 cut(s) 1343
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 4 cut(s) 126, 201, 1792, 1848
BstDEI CTNAG 5 cut(s) 284, 577, 1071, 1319, 2117
BstDSI CCRYGG 1 cut(s) 116
BstF5I GGATG 5 cut(s) 226, 545, 1053, 1295, 1520
BstH2I RGCGCY 1 cut(s) 1957
BstHHI GCGC 1 cut(s) 1956
BstMAI GTCTC 3 cut(s) 287, 939, 1733
BstMCI CGRYCG 1 cut(s) 1809
BstMWI GCNNNNNNNGC 9 cut(s) 121, 190, 205, 615, 630, 1810, 1953, 1982, 2147
BstNI CCWGG 3 cut(s) 17, 205, 736
BstNSI RCATGY 2 cut(s) 115, 1642
BstSCI CCNGG 3 cut(s) 15, 203, 734
BstSFI CTRYAG 2 cut(s) 948, 1509
BstSLI GKGCMC 1 cut(s) 1275
BstV1I GCAGC 8 cut(s) 100, 108, 220, 442, 597, 621, 901, 934
BstV2I GAAGAC 1 cut(s) 310
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
BsuI GTATCC 1 cut(s) 1223
BsuRI GGCC 5 cut(s) 45, 203, 243, 1033, 1804
BtgI CCRYGG 1 cut(s) 116
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 5 cut(s) 226, 545, 1053, 1295, 1520
BtsI GCAGTG 2 cut(s) 1506, 2113
BtsIMutI CAGTG 5 cut(s) 187, 1327, 1506, 1694, 2113
Cac8I GCNNGC 4 cut(s) 126, 201, 1792, 1848
CciI TCATGA 2 cut(s) 1018, 1758
CfoI GCGC 1 cut(s) 1956
Cfr13I GGNCC 2 cut(s) 201, 2071
CseI GACGC 4 cut(s) 40, 257, 601, 816
Csp6I GTAC 9 cut(s) 463, 1344, 1352, 1524, 1661, 1720, 1780, 1838, 1942
CviAII CATG 6 cut(s) 112, 262, 1019, 1282, 1639, 1759
CviQI GTAC 9 cut(s) 463, 1344, 1352, 1524, 1661, 1720, 1780, 1838, 1942
DdeI CTNAG 5 cut(s) 284, 577, 1071, 1319, 2117
DriI GACNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 2 cut(s) 1031, 1802
Eam1104I CTCTTC 3 cut(s) 62, 151, 1917
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 3 cut(s) 62, 151, 1917
Eco130I CCWWGG 2 cut(s) 1138, 1775
Eco32I GATATC 1 cut(s) 1068
Eco47I GGWCC 1 cut(s) 2071
Eco47III AGCGCT 1 cut(s) 1955
Eco88I CYCGRG 3 cut(s) 164, 356, 430
EcoO109I RGGNCCY 1 cut(s) 2071
EcoRII CCWGG 3 cut(s) 15, 203, 734
EcoRV GATATC 1 cut(s) 1068
EcoT14I CCWWGG 2 cut(s) 1138, 1775
EcoT22I ATGCAT 1 cut(s) 1097
ErhI CCWWGG 2 cut(s) 1138, 1775
FaeI CATG 6 cut(s) 115, 265, 1022, 1285, 1642, 1762
FalI AAGNNNNNCTT 2 cut(s) 1158, 1190
FaqI GGGAC 1 cut(s) 2109
FatI CATG 6 cut(s) 111, 261, 1018, 1281, 1638, 1758
FauI CCCGC 1 cut(s) 192
FblI GTMKAC 2 cut(s) 108, 490
FokI GGATG 5 cut(s) 233, 532, 1040, 1282, 1527
FspBI CTAG 3 cut(s) 1644, 1776, 1889
GlaI GCGC 1 cut(s) 1955
HaeII RGCGCY 1 cut(s) 1957
HaeIII GGCC 5 cut(s) 45, 203, 243, 1033, 1804
HapII CCGG 7 cut(s) 42, 82, 240, 303, 332, 1398, 1948
HgaI GACGC 4 cut(s) 40, 257, 601, 816
HhaI GCGC 1 cut(s) 1956
Hin1I GRCGYC 2 cut(s) 23, 249
Hin1II CATG 6 cut(s) 115, 265, 1022, 1285, 1642, 1762
Hin6I GCGC 1 cut(s) 1954
HinP1I GCGC 1 cut(s) 1954
HincII GTYRAC 3 cut(s) 337, 367, 628
HindII GTYRAC 3 cut(s) 337, 367, 628
HindIII AAGCTT 2 cut(s) 842, 1073
HinfI GANTC 8 cut(s) 143, 151, 175, 445, 1564, 1699, 1734, 1863
HpaII CCGG 7 cut(s) 42, 82, 240, 303, 332, 1398, 1948
HphI GGTGA 3 cut(s) 31, 641, 950
Hpy188I TCNGA 9 cut(s) 287, 757, 874, 883, 1058, 1263, 1534, 1557, 1733
Hpy99I CGWCG 5 cut(s) 184, 251, 257, 401, 687
HpyAV CCTTC 9 cut(s) 663, 842, 937, 1244, 1458, 1717, 1873, 1970, 2037
HpyCH4III ACNGT 6 cut(s) 1270, 1390, 1479, 1592, 2062, 2096
HpyCH4IV ACGT 3 cut(s) 23, 399, 1764
HpyCH4V TGCA 7 cut(s) 751, 950, 1095, 1511, 1813, 1846, 1856
HpyF10VI GCNNNNNNNGC 9 cut(s) 121, 190, 205, 615, 630, 1810, 1953, 1982, 2147
HpyF3I CTNAG 5 cut(s) 284, 577, 1071, 1319, 2117
HpySE526I ACGT 3 cut(s) 23, 399, 1764
Hsp92I GRCGYC 2 cut(s) 23, 249
Hsp92II CATG 6 cut(s) 115, 265, 1022, 1285, 1642, 1762
HspAI GCGC 1 cut(s) 1954
Kpn2I TCCGGA 2 cut(s) 81, 1397
LmnI GCTCC 6 cut(s) 606, 874, 1012, 1696, 1777, 2103
Lsp1109I GCAGC 8 cut(s) 100, 108, 220, 442, 597, 621, 901, 934
LweI GCATC 3 cut(s) 706, 1901, 2073
MaeI CTAG 3 cut(s) 1644, 1776, 1889
MaeII ACGT 3 cut(s) 23, 399, 1764
MbiI CCGCTC 1 cut(s) 169
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 1 cut(s) 1275
MluCI AATT 8 cut(s) 274, 1086, 1131, 1548, 1604, 1620, 1966, 2039
MlyI GAGTC 3 cut(s) 137, 160, 1573
MmeI TCCRAC 1 cut(s) 1840
Mph1103I ATGCAT 1 cut(s) 1097
MroI TCCGGA 2 cut(s) 81, 1397
MroXI GAANNNNTTC 4 cut(s) 906, 1191, 1539, 1955
MseI TTAA 5 cut(s) 273, 711, 816, 1571, 1901
MspA1I CMGCKG 1 cut(s) 1162
MspI CCGG 7 cut(s) 42, 82, 240, 303, 332, 1398, 1948
MspR9I CCNGG 3 cut(s) 17, 205, 736
Mva1269I GAATGC 1 cut(s) 588
MvaI CCWGG 3 cut(s) 17, 205, 736
MwoI GCNNNNNNNGC 9 cut(s) 121, 190, 205, 615, 630, 1810, 1953, 1982, 2147
NlaIII CATG 6 cut(s) 115, 265, 1022, 1285, 1642, 1762
NlaIV GGNNCC 3 cut(s) 350, 1272, 2073
NmeAIII GCCGAG 3 cut(s) 117, 154, 2011
NmuCI GTSAC 8 cut(s) 19, 24, 994, 1222, 1504, 1634, 2056, 2108
NsiI ATGCAT 1 cut(s) 1097
NspI RCATGY 2 cut(s) 115, 1642
NspV TTCGAA 1 cut(s) 413
PaeR7I CTCGAG 3 cut(s) 164, 356, 430
PagI TCATGA 2 cut(s) 1018, 1758
PcsI WCGNNNNNNNCGW 3 cut(s) 71, 252, 318
PctI GAATGC 1 cut(s) 588
PdmI GAANNNNTTC 4 cut(s) 906, 1191, 1539, 1955
PfeI GAWTC 5 cut(s) 175, 445, 1699, 1734, 1863
PflFI GACNNNGTC 1 cut(s) 251
Ple19I CGATCG 1 cut(s) 1809
PleI GAGTC 3 cut(s) 137, 159, 1572
PpsI GAGTC 3 cut(s) 137, 159, 1572
PpuMI RGGWCCY 1 cut(s) 2071
PshBI ATTAAT 1 cut(s) 273
PsiI TTATAA 1 cut(s) 1085
Psp5II RGGWCCY 1 cut(s) 2071
Psp6I CCWGG 3 cut(s) 15, 203, 734
PspGI CCWGG 3 cut(s) 15, 203, 734
PspN4I GGNNCC 3 cut(s) 350, 1272, 2073
PspPI GGNCC 2 cut(s) 201, 2071
PspPPI RGGWCCY 1 cut(s) 2071
PspXI VCTCGAGB 3 cut(s) 164, 356, 430
PsrI GAACNNNNNNTAC 2 cut(s) 891, 923
PstI CTGCAG 2 cut(s) 952, 1513
PsuI RGATCY 1 cut(s) 757
PsyI GACNNNGTC 1 cut(s) 251
PvuI CGATCG 1 cut(s) 1809
PvuII CAGCTG 1 cut(s) 1162
RsaI GTAC 9 cut(s) 464, 1345, 1353, 1525, 1662, 1721, 1781, 1839, 1943
RsaNI GTAC 9 cut(s) 463, 1344, 1352, 1524, 1661, 1720, 1780, 1838, 1942
SaqAI TTAA 5 cut(s) 273, 711, 816, 1571, 1901
Sau96I GGNCC 2 cut(s) 201, 2071
ScaI AGTACT 3 cut(s) 1353, 1525, 1943
SchI GAGTC 3 cut(s) 137, 160, 1573
ScrFI CCNGG 3 cut(s) 17, 205, 736
SduI GDGCHC 1 cut(s) 1275
SfaNI GCATC 3 cut(s) 706, 1901, 2073
SfcI CTRYAG 2 cut(s) 948, 1509
Sfr274I CTCGAG 3 cut(s) 164, 356, 430
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 1 cut(s) 2071
SlaI CTCGAG 3 cut(s) 164, 356, 430
SmlI CTYRAG 4 cut(s) 164, 194, 356, 430
SmoI CTYRAG 4 cut(s) 164, 194, 356, 430
Sse9I AATT 8 cut(s) 274, 1086, 1131, 1548, 1604, 1620, 1966, 2039
SsiI CCGC 8 cut(s) 12, 169, 185, 199, 265, 719, 961, 1415
SspI AATATT 1 cut(s) 1444
SspMI CTAG 3 cut(s) 1644, 1776, 1889
StyD4I CCNGG 3 cut(s) 15, 203, 734
StyI CCWWGG 2 cut(s) 1138, 1775
TaaI ACNGT 6 cut(s) 1270, 1390, 1479, 1592, 2062, 2096
TaiI ACGT 3 cut(s) 26, 402, 1767
TasI AATT 8 cut(s) 274, 1086, 1131, 1548, 1604, 1620, 1966, 2039
TatI WGTACW 5 cut(s) 1343, 1351, 1523, 1660, 1941
TauI GCSGC 3 cut(s) 172, 187, 268
TfiI GAWTC 5 cut(s) 175, 445, 1699, 1734, 1863
Tru1I TTAA 5 cut(s) 273, 711, 816, 1571, 1901
Tru9I TTAA 5 cut(s) 273, 711, 816, 1571, 1901
TscAI CASTG 5 cut(s) 194, 1327, 1513, 1701, 2113
TseFI GTSAC 8 cut(s) 19, 24, 994, 1222, 1504, 1634, 2056, 2108
TseI GCWGC 8 cut(s) 88, 121, 208, 455, 585, 609, 914, 947
Tsp45I GTSAC 8 cut(s) 19, 24, 994, 1222, 1504, 1634, 2056, 2108
TspGWI ACGGA 2 cut(s) 168, 230
TspRI CASTG 5 cut(s) 194, 1327, 1513, 1701, 2113
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 2071
VspI ATTAAT 1 cut(s) 273
XapI RAATTY 3 cut(s) 1604, 1966, 2039
XceI RCATGY 2 cut(s) 115, 1642
XhoI CTCGAG 3 cut(s) 164, 356, 430
XmaJI CCTAGG 1 cut(s) 1775
XmiI GTMKAC 2 cut(s) 108, 490
XmnI GAANNNNTTC 4 cut(s) 906, 1191, 1539, 1955
XspI CTAG 3 cut(s) 1644, 1776, 1889
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 3 cut(s) 1353, 1525, 1943
Zsp2I ATGCAT 1 cut(s) 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.