Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0005229

Physical Location & Seq

Genomic Coordinates Reverse (-)

3750301 .. 3754254

Gene Structure 3954 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g05850.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2154 bp

ATGCTTGTCTCCGCCCTGCTGACGTCACTCGCCATCAACTCCGGCCTCTGCGTCCTCTTCTTCACGCTCTACTCGATCCTCCGGAAGCAGCCGAGCAACTATGAGGTGTACATACCGCGGCTGCTCGCCGAGGGGGAGTCCGACAAGTCGAGTCGTTTCAACCTCGAGCGGCTGATTCCGTCGCCGTACTGGGTCAAGAGGGCCTGGCAGCTCACTGAGGACGACCTGCTGTCGTCCTCCGGCCTCGACGCCGTCGTTTTCATGCGGCTTATCAATTTTAGTCTCAGAGTGTTCCTGTTCGCCGGAGTTATCGGGATATTCGTCCTGCTTCCGGTCAACTGCTCCGGCAACCAGCTCGAGTATGTTGACTTCGCCGATCTGTCGAATAACTCTCTCGACGTGTTTACGATTTCGAATGTGAACAATGGCTCAAGCAAATTATGGATTCACTTTGCTGCTGTGTACCTTGTCACCATCTTTGTCTGCTGTCTACTTTATTATGAATTTAGATATATTTCTCAAAGACGGCTTGATTATTTTCAGTCATCCAAGCCTCAACCGCATCAGTTCACAATCTTAGTTCGCAGCATTCCTGTTCCTTCTGGGAGCAGTGTCAGTGAAAAAGTTGACAGCTTCTTTAGGGAGTATCACCCTTCAACGTATTTGTCACATATTGTTGTTCGTCGAACAAACAAAGTTAAAAAACTCCTTAAAGATACGAAAAAACTGTATACCAGGCTCCTTCACTTGCAATCGGATCCAAACCAGCAAAAGTATACACGATCCTGTTGTTTTGGATTGTTTGGACACAAGGTTAATCTTGTGGAACAGTATGAAAAGAAGCTTGAAGACGCAGAGGAGAATGTGAGATTGGAGCAATCAGAGGCTTCATTAGGAGGAGAAGAAGTTCGATCTGCTTTTGTTTCCTTCAAGTCTCGGTATGGTGCTGCAATTGCTCACCACTTGCAACAATCAACCAATCCTACCAACTGGGTCACAGAGCAAGCTCCTGAACCTCGTGATGTTTACTGGCCGTTCTTTTCGTCATCTTTCATCCGAAGATGGATATCTAAGCTTGTGGTTATACTTTTATGCATTGTCCTAACAATCTTGTTCCTTATTCCTGTTGTACTTGTCCAAGGTCTTACCAACCTAAACCAGCTGGAAGCTTGGCTTCCCTTTCTCGAAAGTGTTCTAACCATAACATTTGTTAGTCAAGTCATTACAGGATACCTTCCCAGTCTTATTCTTCTATTATTTCTGAAAATAGTGCCCCCCGCCATGGAGTTCCTTTCATCCATTCAAGGATATGTTTCTCTCAGTGATATACAAAAGAGTGCGTGTACAAAAGTACTTTGGTTCACGATATGGAACATTTTTTTTGCTACTGTATTTTCCGGATCAGTTCTCTCTAAGGTTTATCTCTTTCTTGACCCCAAGAATATTCCTTCAAAGCTAGGCGTTTCTGTTCCAGCACAGTCGTCTTTTTTCATTGCTTATGTCGTCACGTCAGGATGGACAAGTACTTCATCAGAACTCTTTCGCATAATTCCTCTGATATGGAGTCTGTTAAAAAGACCTTTTACCGACAGTAAAGATGATGAATTTGAAGTTCCAGCAATGAATTACCATAATCACATGCCAAGAGTTCTTTTCTTTGTACTTCTCGGCATCACATACTTCTTTCTAGCTCCACTGATTCTTCCCTTCCTCTTAGTGTACCTCTGTCTTGGATTCATCATCTTTCGCAACCAGTTCATAAACGTATATGCTCCTAGGTTTGAAACTGCAGGGAAGTTTTGGCCGATTGTGCATAATTCAATGATCTTCTCTCTGGTACTTATGCACGCTATTGCAGTTGGAATCTTTACAATCAAGAAGGTCAATCTAGCATCGACATTAACTTTTCCTCTTCCTGTATTCACACTTCTCTTCAATGAGTACTGCCGGAAGCGCTTCCTTCCAAATTTCGTTGCTTACCCTGCTGAGAGTTTGATACGAAAGGACAGGCAAGATGAGAACGATCCTACAATGCCTGAATTTCTCGATAAGTTGGTCACGGTCTATCAGGACCCTGCTTTGATGCCTATCAACTATTCTGGGAGCAATGACAGACTCCGCAGTCCTCTTTTATCTTCTGCTCGTCAAGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

718

Amino Acids

82.08

Weight (kDa)

8.97

Isoelectric Point (pI)

45.44

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69450 AT1G69450 AT1G69450
fragaria_vesca	FvH4_4g30740 FvH4_4g30740
malus_domestica	MD13G1068300.v1.1 MD16G1067100.v1.1
prunus_persica	Prupe.1G279800_v2.0.a1 Prupe.1G279800_v2.0.a1 Prupe.1G279800_v2.0.a1 Prupe.1G279800_v2.0.a1 Prupe.1G279800_v2.0.a1
pyrus_communis	pycom13g06010 pycom16g05850
rosa_chinensis	RchiOBHm_Chr4g0439131
rosa_laevigata	RLG00000006295
rosa_multiflora	Rmu_sc0002236.1_g000001 Rmu_sc0005346.1_g000005 Rmu_sc0027506.1_g000002
rosa_roxburghii	Rroxscaffold_5G00380120
rosa_rugosa	Rorug04G0315900 Rorug04G0315900
rosa_samantha	Rh4AG366800 Rh4BG378300 Rh4CG392400 Rh4CG392500 Rh4DG372900
rosa_wichuraiana	Rw0G007500 Rw0G011730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2121
AatII	GACGTC	1 cut(s)	26
Acc36I	ACCTGC	1 cut(s)	234
AccBSI	CCGCTC	1 cut(s)	169
AccI	GTMKAC	3 cut(s)	490, 731, 776
AccII	CGCG	1 cut(s)	118
AccIII	TCCGGA	2 cut(s)	81, 1397
AciI	CCGC	8 cut(s)	12, 116, 118, 169, 265, 560, 1278, 2119
AclWI	GGATC	6 cut(s)	70, 752, 765, 777, 1408, 2018
AcoI	YGGCCR	2 cut(s)	1031, 1802
AcsI	RAATTY	4 cut(s)	503, 1604, 1966, 2039
AcyI	GRCGYC	2 cut(s)	23, 249
AfeI	AGCGCT	1 cut(s)	1955
AfiI	CCNNNNNNNGG	2 cut(s)	331, 1282
AflIII	ACRYGT	1 cut(s)	399
AhdI	GACNNNNNGTC	1 cut(s)	229
AjiI	CACGTC	2 cut(s)	400, 1509
AjnI	CCWGG	2 cut(s)	203, 734
AjuI	GAANNNNNNNTTGG	2 cut(s)	854, 886
AloI	GAACNNNNNNTCC	2 cut(s)	891, 923
Alw26I	GTCTC	3 cut(s)	13, 287, 939
AlwI	GGATC	6 cut(s)	70, 752, 765, 777, 1408, 2018
Ama87I	CYCGRG	2 cut(s)	164, 356
Aor13HI	TCCGGA	2 cut(s)	81, 1397
Aor51HI	AGCGCT	1 cut(s)	1955
AoxI	GGCC	5 cut(s)	43, 201, 241, 1031, 1802
ApeKI	GCWGC	6 cut(s)	88, 121, 208, 455, 585, 947
ApoI	RAATTY	4 cut(s)	503, 1604, 1966, 2039
Asp700I	GAANNNNTTC	4 cut(s)	906, 1191, 1539, 1955
AspA2I	CCTAGG	1 cut(s)	1775
AspLEI	GCGC	1 cut(s)	1956
AspS9I	GGNCC	2 cut(s)	201, 2071
AsuHPI	GGTGA	3 cut(s)	463, 641, 950
AsuII	TTCGAA	1 cut(s)	413
AvaI	CYCGRG	2 cut(s)	164, 356
AvaII	GGWCC	1 cut(s)	2071
AvrII	CCTAGG	1 cut(s)	1775
BaeGI	GKGCMC	1 cut(s)	1275
BamHI	GGATCC	1 cut(s)	757
BarI	GAAGNNNNNNTAC	4 cut(s)	353, 385, 1911, 1943
BauI	CACGAG	1 cut(s)	1017
BbsI	GAAGAC	1 cut(s)	855
BbvI	GCAGC	6 cut(s)	100, 108, 220, 442, 597, 934
BccI	CCATC	4 cut(s)	41, 482, 1056, 1509
BceAI	ACGGC	4 cut(s)	169, 236, 542, 1018
BcgI	CGANNNNNNTGC	2 cut(s)	337, 371
BciT130I	CCWGG	2 cut(s)	205, 736
BciVI	GTATCC	1 cut(s)	1223
BcoDI	GTCTC	3 cut(s)	13, 287, 939
BfaI	CTAG	4 cut(s)	1457, 1688, 1776, 1889
BfmI	CTRYAG	1 cut(s)	1788
BfoI	RGCGCY	1 cut(s)	1957
BfuAI	ACCTGC	1 cut(s)	234
BfuI	GTATCC	1 cut(s)	1223
BisI	GCNGC	9 cut(s)	89, 119, 122, 170, 209, 266, 456, 586, 948
BlnI	CCTAGG	1 cut(s)	1775
BlsI	GCNGC	9 cut(s)	90, 120, 123, 171, 210, 267, 457, 587, 949
BmcAI	AGTACT	3 cut(s)	1353, 1525, 1943
Bme1390I	CCNGG	2 cut(s)	205, 736
Bme18I	GGWCC	1 cut(s)	2071
BmeRI	GACNNNNNGTC	1 cut(s)	229
BmeT110I	CYCGRG	2 cut(s)	164, 356
BmgBI	CACGTC	2 cut(s)	400, 1509
BmgT120I	GGNCC	2 cut(s)	201, 2071
BmiI	GGNNCC	3 cut(s)	740, 759, 2073
BmrFI	CCNGG	2 cut(s)	205, 736
BmrI	ACTGGG	3 cut(s)	199, 1000, 1233
BmsI	GCATC	4 cut(s)	571, 1680, 1901, 2073
BmuI	ACTGGG	3 cut(s)	199, 1000, 1233
BpiI	GAAGAC	1 cut(s)	855
Bpu14I	TTCGAA	1 cut(s)	413
BpuEI	CTTGAG	1 cut(s)	415
BsaBI	GATNNNNATC	2 cut(s)	1066, 2087
BsaHI	GRCGYC	2 cut(s)	23, 249
BsaJI	CCNNGG	5 cut(s)	116, 129, 1138, 1281, 1775
BsaWI	WCCGGW	3 cut(s)	81, 331, 1397
BsaXI	ACNNNNNCTCC	2 cut(s)	891, 921
Bsc4I	CCNNNNNNNGG	2 cut(s)	331, 1282
Bse1I	ACTGG	5 cut(s)	194, 995, 1034, 1239, 1753
Bse3DI	GCAATG	3 cut(s)	1491, 1626, 2113
Bse8I	GATNNNNATC	2 cut(s)	1066, 2087
BseAI	TCCGGA	2 cut(s)	81, 1397
BseBI	CCWGG	2 cut(s)	205, 736
BseDI	CCNNGG	5 cut(s)	116, 129, 1138, 1281, 1775
BseGI	GGATG	4 cut(s)	545, 1053, 1295, 1520
BseJI	GATNNNNATC	2 cut(s)	1066, 2087
BseLI	CCNNNNNNNGG	2 cut(s)	331, 1282
BseMI	GCAATG	3 cut(s)	1491, 1626, 2113
BseMII	CTCAG	4 cut(s)	207, 298, 1333, 1977
BseNI	ACTGG	5 cut(s)	194, 995, 1034, 1239, 1753
BseRI	GAGGAG	2 cut(s)	872, 912
BseSI	GKGCMC	1 cut(s)	1275
BseXI	GCAGC	6 cut(s)	100, 108, 220, 442, 597, 934
Bsh1236I	CGCG	1 cut(s)	118
BshFI	GGCC	5 cut(s)	45, 203, 243, 1033, 1804
BsiHKCI	CYCGRG	2 cut(s)	164, 356
BsiSI	CCGG	8 cut(s)	42, 82, 240, 303, 332, 345, 1398, 1948
BslI	CCNNNNNNNGG	2 cut(s)	331, 1282
BsmAI	GTCTC	3 cut(s)	13, 287, 939
BsmI	GAATGC	1 cut(s)	588
BsnI	GGCC	5 cut(s)	45, 203, 243, 1033, 1804
BsoBI	CYCGRG	2 cut(s)	164, 356
Bsp119I	TTCGAA	1 cut(s)	413
Bsp1286I	GDGCHC	1 cut(s)	1275
Bsp13I	TCCGGA	2 cut(s)	81, 1397
Bsp1407I	TGTACA	2 cut(s)	108, 1343
Bsp143I	GATC	8 cut(s)	75, 376, 757, 782, 911, 1400, 1824, 2023
Bsp19I	CCATGG	1 cut(s)	1281
BspACI	CCGC	8 cut(s)	12, 116, 118, 169, 265, 560, 1278, 2119
BspANI	GGCC	5 cut(s)	45, 203, 243, 1033, 1804
BspCNI	CTCAG	4 cut(s)	208, 297, 1332, 1978
BspEI	TCCGGA	2 cut(s)	81, 1397
BspFNI	CGCG	1 cut(s)	118
BspLI	GGNNCC	3 cut(s)	740, 759, 2073
BspMAI	CTGCAG	1 cut(s)	1792
BspMI	ACCTGC	1 cut(s)	234
BspPI	GGATC	6 cut(s)	70, 752, 765, 777, 1408, 2018
BspT104I	TTCGAA	1 cut(s)	413
BsrBI	CCGCTC	1 cut(s)	169
BsrDI	GCAATG	3 cut(s)	1491, 1626, 2113
BsrGI	TGTACA	2 cut(s)	108, 1343
BsrI	ACTGG	5 cut(s)	194, 995, 1034, 1239, 1753
BssECI	CCNNGG	5 cut(s)	116, 129, 1138, 1281, 1775
BssMI	GATC	8 cut(s)	75, 376, 757, 782, 911, 1400, 1824, 2023
BssNAI	GTATAC	2 cut(s)	732, 777
BssNI	GRCGYC	2 cut(s)	23, 249
BssSI	CACGAG	1 cut(s)	1017
BssT1I	CCWWGG	3 cut(s)	1138, 1281, 1775
Bst1107I	GTATAC	2 cut(s)	732, 777
Bst2BI	CACGAG	1 cut(s)	1017
Bst2UI	CCWGG	2 cut(s)	205, 736
Bst4CI	ACNGT	6 cut(s)	729, 831, 1390, 1479, 1592, 2062
Bst6I	CTCTTC	3 cut(s)	62, 1917, 1937
BstACI	GRCGYC	2 cut(s)	23, 249
BstAUI	TGTACA	2 cut(s)	108, 1343
BstBI	TTCGAA	1 cut(s)	413
BstC8I	GCNNGC	3 cut(s)	126, 1005, 1848
BstDEI	CTNAG	8 cut(s)	216, 284, 577, 1071, 1319, 1413, 1714, 1986
BstDSI	CCRYGG	2 cut(s)	116, 1281
BstF5I	GGATG	4 cut(s)	545, 1053, 1295, 1520
BstFNI	CGCG	1 cut(s)	118
BstH2I	RGCGCY	1 cut(s)	1957
BstHHI	GCGC	1 cut(s)	1956
BstKTI	GATC	8 cut(s)	78, 379, 760, 785, 914, 1403, 1827, 2026
BstMAI	GTCTC	3 cut(s)	13, 287, 939
BstMBI	GATC	8 cut(s)	75, 376, 757, 782, 911, 1400, 1824, 2023
BstMWI	GCNNNNNNNGC	6 cut(s)	559, 953, 1810, 1953, 1982, 2147
BstNI	CCWGG	2 cut(s)	205, 736
BstNSI	RCATGY	1 cut(s)	1642
BstSCI	CCNGG	2 cut(s)	203, 734
BstSFI	CTRYAG	1 cut(s)	1788
BstSLI	GKGCMC	1 cut(s)	1275
BstUI	CGCG	1 cut(s)	118
BstV1I	GCAGC	6 cut(s)	100, 108, 220, 442, 597, 934
BstV2I	GAAGAC	1 cut(s)	855
BstX2I	RGATCY	1 cut(s)	757
BstYI	RGATCY	1 cut(s)	757
BstZ17I	GTATAC	2 cut(s)	732, 777
BsuI	GTATCC	1 cut(s)	1223
BsuRI	GGCC	5 cut(s)	45, 203, 243, 1033, 1804
BtgI	CCRYGG	2 cut(s)	116, 1281
BtrI	CACGTC	2 cut(s)	400, 1509
BtsCI	GGATG	4 cut(s)	545, 1053, 1295, 1520
BtsI	GCAGTG	1 cut(s)	616
BtsIMutI	CAGTG	5 cut(s)	213, 616, 622, 1327, 1694
BveI	ACCTGC	1 cut(s)	234
Cac8I	GCNNGC	3 cut(s)	126, 1005, 1848
CfoI	GCGC	1 cut(s)	1956
Cfr13I	GGNCC	2 cut(s)	201, 2071
Cfr42I	CCGCGG	1 cut(s)	119
CseI	GACGC	3 cut(s)	40, 257, 860
CviAII	CATG	3 cut(s)	262, 1282, 1639
DdeI	CTNAG	8 cut(s)	216, 284, 577, 1071, 1319, 1413, 1714, 1986
DpnI	GATC	8 cut(s)	77, 378, 759, 784, 913, 1402, 1826, 2025
DpnII	GATC	8 cut(s)	75, 376, 757, 782, 911, 1400, 1824, 2023
DrdI	GACNNNNNNGTC	1 cut(s)	2121
DriI	GACNNNNNGTC	1 cut(s)	229
DseDI	GACNNNNNNGTC	1 cut(s)	2121
EaeI	YGGCCR	2 cut(s)	1031, 1802
Eam1104I	CTCTTC	3 cut(s)	62, 1917, 1937
Eam1105I	GACNNNNNGTC	1 cut(s)	229
EarI	CTCTTC	3 cut(s)	62, 1917, 1937
Eco130I	CCWWGG	3 cut(s)	1138, 1281, 1775
Eco32I	GATATC	1 cut(s)	1068
Eco47I	GGWCC	1 cut(s)	2071
Eco47III	AGCGCT	1 cut(s)	1955
Eco88I	CYCGRG	2 cut(s)	164, 356
EcoO109I	RGGNCCY	2 cut(s)	201, 2071
EcoRII	CCWGG	2 cut(s)	203, 734
EcoRV	GATATC	1 cut(s)	1068
EcoT14I	CCWWGG	3 cut(s)	1138, 1281, 1775
EcoT22I	ATGCAT	1 cut(s)	1097
ErhI	CCWWGG	3 cut(s)	1138, 1281, 1775
FaeI	CATG	3 cut(s)	265, 1285, 1642
FalI	AAGNNNNNCTT	2 cut(s)	1158, 1190
FatI	CATG	3 cut(s)	261, 1281, 1638
FauI	CCCGC	1 cut(s)	1285
FblI	GTMKAC	3 cut(s)	490, 731, 776
Fnu4HI	GCNGC	9 cut(s)	89, 119, 122, 170, 209, 266, 456, 586, 948
FokI	GGATG	4 cut(s)	532, 1040, 1282, 1527
Fsp4HI	GCNGC	9 cut(s)	89, 119, 122, 170, 209, 266, 456, 586, 948
FspBI	CTAG	4 cut(s)	1457, 1688, 1776, 1889
GlaI	GCGC	1 cut(s)	1955
GluI	GCNGC	9 cut(s)	89, 119, 122, 170, 209, 266, 456, 586, 948
HaeII	RGCGCY	1 cut(s)	1957
HaeIII	GGCC	5 cut(s)	45, 203, 243, 1033, 1804
HapII	CCGG	8 cut(s)	42, 82, 240, 303, 332, 345, 1398, 1948
HgaI	GACGC	3 cut(s)	40, 257, 860
HhaI	GCGC	1 cut(s)	1956
Hin1I	GRCGYC	2 cut(s)	23, 249
Hin1II	CATG	3 cut(s)	265, 1285, 1642
Hin6I	GCGC	1 cut(s)	1954
HinP1I	GCGC	1 cut(s)	1954
HincII	GTYRAC	3 cut(s)	337, 367, 628
HindII	GTYRAC	3 cut(s)	337, 367, 628
HindIII	AAGCTT	3 cut(s)	842, 1073, 1167
HinfI	GANTC	9 cut(s)	137, 151, 175, 445, 1564, 1699, 1734, 1863, 2115
HpaII	CCGG	8 cut(s)	42, 82, 240, 303, 332, 345, 1398, 1948
HphI	GGTGA	3 cut(s)	463, 641, 950
Hpy188I	TCNGA	8 cut(s)	142, 287, 757, 883, 1058, 1263, 1534, 1557
Hpy99I	CGWCG	5 cut(s)	184, 251, 257, 401, 687
HpyAV	CCTTC	9 cut(s)	609, 663, 752, 937, 1244, 1458, 1717, 1873, 1970
HpyCH4III	ACNGT	6 cut(s)	729, 831, 1390, 1479, 1592, 2062
HpyCH4IV	ACGT	5 cut(s)	23, 399, 659, 1508, 1764
HpyCH4V	TGCA	8 cut(s)	751, 950, 967, 1095, 1790, 1813, 1846, 1856
HpyF10VI	GCNNNNNNNGC	6 cut(s)	559, 953, 1810, 1953, 1982, 2147
HpyF3I	CTNAG	8 cut(s)	216, 284, 577, 1071, 1319, 1413, 1714, 1986
HpySE526I	ACGT	5 cut(s)	23, 399, 659, 1508, 1764
Hsp92I	GRCGYC	2 cut(s)	23, 249
Hsp92II	CATG	3 cut(s)	265, 1285, 1642
HspAI	GCGC	1 cut(s)	1954
Kpn2I	TCCGGA	2 cut(s)	81, 1397
KspI	CCGCGG	1 cut(s)	119
Kzo9I	GATC	8 cut(s)	75, 376, 757, 782, 911, 1400, 1824, 2023
LmnI	GCTCC	8 cut(s)	347, 606, 744, 874, 1012, 1696, 1777, 2103
Lsp1109I	GCAGC	6 cut(s)	100, 108, 220, 442, 597, 934
LweI	GCATC	4 cut(s)	571, 1680, 1901, 2073
MaeI	CTAG	4 cut(s)	1457, 1688, 1776, 1889
MaeII	ACGT	5 cut(s)	23, 399, 659, 1508, 1764
MaeIII	GTNAC	6 cut(s)	24, 469, 666, 994, 1504, 2056
MalI	GATC	8 cut(s)	77, 378, 759, 784, 913, 1402, 1826, 2025
MbiI	CCGCTC	1 cut(s)	169
MboI	GATC	8 cut(s)	75, 376, 757, 782, 911, 1400, 1824, 2023
MfeI	CAATTG	1 cut(s)	951
MflI	RGATCY	1 cut(s)	757
MhlI	GDGCHC	1 cut(s)	1275
MlyI	GAGTC	4 cut(s)	146, 160, 1573, 2109
MmeI	TCCRAC	2 cut(s)	165, 1840
Mph1103I	ATGCAT	1 cut(s)	1097
MroI	TCCGGA	2 cut(s)	81, 1397
MroXI	GAANNNNTTC	4 cut(s)	906, 1191, 1539, 1955
MseI	TTAA	5 cut(s)	699, 711, 816, 1571, 1901
MspA1I	CMGCKG	2 cut(s)	118, 1162
MspI	CCGG	8 cut(s)	42, 82, 240, 303, 332, 345, 1398, 1948
MspR9I	CCNGG	2 cut(s)	205, 736
MunI	CAATTG	1 cut(s)	951
Mva1269I	GAATGC	1 cut(s)	588
MvaI	CCWGG	2 cut(s)	205, 736
MvnI	CGCG	1 cut(s)	118
MwoI	GCNNNNNNNGC	6 cut(s)	559, 953, 1810, 1953, 1982, 2147
NcoI	CCATGG	1 cut(s)	1281
NdeII	GATC	8 cut(s)	75, 376, 757, 782, 911, 1400, 1824, 2023
NlaIII	CATG	3 cut(s)	265, 1285, 1642
NlaIV	GGNNCC	3 cut(s)	740, 759, 2073
NmeAIII	GCCGAG	3 cut(s)	117, 154, 1647
NmuCI	GTSAC	6 cut(s)	24, 469, 666, 994, 1504, 2056
NsiI	ATGCAT	1 cut(s)	1097
NspI	RCATGY	1 cut(s)	1642
NspV	TTCGAA	1 cut(s)	413
PaeR7I	CTCGAG	2 cut(s)	164, 356
PcsI	WCGNNNNNNNCGW	3 cut(s)	71, 252, 318
PctI	GAATGC	1 cut(s)	588
PdmI	GAANNNNTTC	4 cut(s)	906, 1191, 1539, 1955
PfeI	GAWTC	5 cut(s)	175, 445, 1699, 1734, 1863
PflFI	GACNNNGTC	1 cut(s)	251
PkrI	GCNGC	9 cut(s)	90, 120, 123, 171, 210, 267, 457, 587, 949
PleI	GAGTC	4 cut(s)	145, 159, 1572, 2109
PpsI	GAGTC	4 cut(s)	145, 159, 1572, 2109
PpuMI	RGGWCCY	1 cut(s)	2071
Psp5II	RGGWCCY	1 cut(s)	2071
Psp6I	CCWGG	2 cut(s)	203, 734
PspGI	CCWGG	2 cut(s)	203, 734
PspN4I	GGNNCC	3 cut(s)	740, 759, 2073
PspPI	GGNCC	2 cut(s)	201, 2071
PspPPI	RGGWCCY	1 cut(s)	2071
PspXI	VCTCGAGB	2 cut(s)	164, 356
PstI	CTGCAG	1 cut(s)	1792
PsuI	RGATCY	1 cut(s)	757
PsyI	GACNNNGTC	1 cut(s)	251
PvuII	CAGCTG	1 cut(s)	1162
SacII	CCGCGG	1 cut(s)	119
SaqAI	TTAA	5 cut(s)	699, 711, 816, 1571, 1901
SatI	GCNGC	9 cut(s)	89, 119, 122, 170, 209, 266, 456, 586, 948
Sau3AI	GATC	8 cut(s)	75, 376, 757, 782, 911, 1400, 1824, 2023
Sau96I	GGNCC	2 cut(s)	201, 2071
ScaI	AGTACT	3 cut(s)	1353, 1525, 1943
SchI	GAGTC	4 cut(s)	146, 160, 1573, 2109
ScrFI	CCNGG	2 cut(s)	205, 736
SduI	GDGCHC	1 cut(s)	1275
SfaNI	GCATC	4 cut(s)	571, 1680, 1901, 2073
SfcI	CTRYAG	1 cut(s)	1788
Sfr274I	CTCGAG	2 cut(s)	164, 356
Sfr303I	CCGCGG	1 cut(s)	119
SfuI	TTCGAA	1 cut(s)	413
SgrBI	CCGCGG	1 cut(s)	119
SinI	GGWCC	1 cut(s)	2071
SlaI	CTCGAG	2 cut(s)	164, 356
SmlI	CTYRAG	3 cut(s)	164, 356, 430
SmoI	CTYRAG	3 cut(s)	164, 356, 430
SsiI	CCGC	8 cut(s)	12, 116, 118, 169, 265, 560, 1278, 2119
SspI	AATATT	1 cut(s)	1444
SspMI	CTAG	4 cut(s)	1457, 1688, 1776, 1889
StyD4I	CCNGG	2 cut(s)	203, 734
StyI	CCWWGG	3 cut(s)	1138, 1281, 1775
TaaI	ACNGT	6 cut(s)	729, 831, 1390, 1479, 1592, 2062
TaiI	ACGT	5 cut(s)	26, 402, 662, 1511, 1767
TatI	WGTACW	7 cut(s)	108, 1129, 1343, 1351, 1523, 1660, 1941
TauI	GCSGC	3 cut(s)	121, 172, 268
TfiI	GAWTC	5 cut(s)	175, 445, 1699, 1734, 1863
Tru1I	TTAA	5 cut(s)	699, 711, 816, 1571, 1901
Tru9I	TTAA	5 cut(s)	699, 711, 816, 1571, 1901
TscAI	CASTG	5 cut(s)	220, 616, 622, 1327, 1701
TseFI	GTSAC	6 cut(s)	24, 469, 666, 994, 1504, 2056
TseI	GCWGC	6 cut(s)	88, 121, 208, 455, 585, 947
Tsp45I	GTSAC	6 cut(s)	24, 469, 666, 994, 1504, 2056
TspGWI	ACGGA	1 cut(s)	168
TspRI	CASTG	5 cut(s)	220, 616, 622, 1327, 1701
Tth111I	GACNNNGTC	1 cut(s)	251
VpaK11BI	GGWCC	1 cut(s)	2071
XapI	RAATTY	4 cut(s)	503, 1604, 1966, 2039
XceI	RCATGY	1 cut(s)	1642
XhoI	CTCGAG	2 cut(s)	164, 356
XmaJI	CCTAGG	1 cut(s)	1775
XmiI	GTMKAC	3 cut(s)	490, 731, 776
XmnI	GAANNNNTTC	4 cut(s)	906, 1191, 1539, 1955
XspI	CTAG	4 cut(s)	1457, 1688, 1776, 1889
ZraI	GACGTC	1 cut(s)	24
ZrmI	AGTACT	3 cut(s)	1353, 1525, 1943
Zsp2I	ATGCAT	1 cut(s)	1097

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.