RchiOBHm_Chr4g0439131

membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005229
Physical Location & Seq
Reverse (-)
61574592 .. 61579786
5195 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40720

Sequence Viewer

Length: 2160 bp
ATGCTCGTCTCGGCGCTGGTGACGTCACTGGCCATAAACTCCGGCCTGTGCGTTCTCTTCTTCACGCTCTACTCCATCCTGAGAAAACAGCCGAGCAACTCCGAGGTCTACATACCGCGAGTTTTGGCCGAAGGCGGGTACAAGAAGAGTAGCCATTTCAATCTAGAGAGGCTGATTCCTTCTCCCGATTGGCTCCGAAGGGCCTGGAAGCTCACCGAGGACGATTTGCTCGAGGCGTCGGGTTTGGACGCCGTGGTTTTTATGCGGCTCATCAATTTCAGCCTGAGAGTGTTTCTGTTCGCCGGAGTTATTGGCGTTTTCGTGCTGCTTCCGGTGAACTGCTCCGGCGATGCGCTCGAGTACGTTGACTTCGCCGATCTGTCTAATAACTCATTGGATGTGTTCAGTATTTCAAATGTTGCTAGCGGCTCAAGCTTGTTATGGATTCACTTTTCGGCAGTGTACCTTGTCACCATCTTTATCTGCTGTCTACTCTATTATGAATATAGATATATCTGTGAAAAGAGGACCAGTTATTTTCTTGCATCCAAGCCACAACCACATCAGTTCACCATCCTAGTTCGCAGCATTCCTGTTCCTGTAGGGAGCAGCGTCAGTGATAGTGTTGAGAGATTCTTTAGCGAGTATCATCCTTCTACATATCTGTCACATAGTGTTGTTCGTCGAACAAACAAACTTCAGAATCTCATTAGTGATGCAAAAAAATTGTATAGCAGACTCGTTCACTTGAAGTCAGATCCCAATAAGCAAAAGTATAAACGTTCCGGTACTTTTGGGTTGTTCGGACCCAAGGTTAATCTACAAGATCATTACAAAAAGACGCTTGAGGACATAGAGGAGAATGTGAGATTGGAGCAATCAGAGGTTTCGTTAGCAGGAGAAGAAGTTCGAGCTGCGTTTGTGTCCTTCAAGTCTCGGTATGGTGCCGCAATTGCTTTCCACTTGCAACAATCAACCAATCCTACGCGATGGGTCACAGAGCAAGCTCCTGAACCACATGATGTTTACTGGCCCTTCTTTTCCTCATCATTCATTCGAAGATGGATATCTAAGCTGGTGGTTATAATTGCTTGCATTCTTCTTACAATTCTATTCCTCATTCCTGTTGTACTTGTTCAAGGTCTCACTAACTTAAGCCAGTTGGAAATCTACTTTCCCTTCCTCACGAGCATTCTAACCATAACATTTGTCAGTGAAGTCATTACAGGATACCTTCCCAGTCTCATTCTTCTGTTATTTCTGAAAATGGTGCCTCCTGTCATGGAGGGTTTTTCATCCATTCAAGGCTATATTTCTAATAGTGCAATACAAAAGAGTGCGTGTTCAAAAGTACTTTGGTTCACAGTATGGAACATTTTTTTTGCTACTGTATTTTCTGGATCCGTATTCTACAAGATTTCCATCTTTCTTGATCCTAAGAACATTCCTGCAAAGCTAGCTTCTGCTGTTCCTGCACAGGCGTCCTTTTTCATTGCATACGTTGTCACATCAGGATGGACGAGTACTTCATCAGAACTCTTTCGCATAATTCCTTTACTTTGGAGTCTAATGAAAAGACCTTTTACTGACAGTAAAGATGATGAACTTGAAGTTCCAGCCATTCCTTACCATAGTCACATGCCAAGAATTCTCTTTTTTGTACTTCTTGGTATCACATACTTCTTTCTAGCTCCACTAATTCTGCCCTTCCTATTGGTGTACCTCTGTCTTGGATACATAATCTATCGTAATCAGTTCATAAATGTATATGCGCCTCGGTATGAAACTGCAGGGAAGTTCTGGCCAATCGCTCACAATTCAGTTATATTTTCTCTGGTACTTATGCACGCTATTGCAGTTGGAGTCTTTACACTGAAGAGTATCCCTGTAGCATCAACCCTAACTTTCCCTCTTCCTGTGTTCACACTTCTTTTTAATGAGTACTGCCGGAAACGCTTCCTGCCCAATTTTATTGCCTATCCTGCTGAGAGTTTGTTAAAAAAGGACAGGCAAGATGAGAATGATCCTACTATGCCTGAATTTTACAGTAAATTGGTTGGTGTCTATCAAGACCCGGCTTTGATGCCTATCAATTTTTCTGCGAACGGTGACAGACTCAACAGCCCTCTTCTATCTGCTTCTTCTTCAAGTCATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

719

Amino Acids

81.46

Weight (kDa)

8.85

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 168 1.5e-37 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 189 - 345 1.7e-37 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 358 - 623 3.6e-75 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 2 cut(s) 944, 1270
AccI GTMKAC 2 cut(s) 108, 490
AccII CGCG 2 cut(s) 118, 988
AciI CCGC 5 cut(s) 116, 135, 265, 426, 948
AclI AACGTT 1 cut(s) 781
AclWI GGATC 5 cut(s) 752, 1395, 1408, 1427, 2018
AcoI YGGCCR 3 cut(s) 30, 126, 1802
AcsI RAATTY 2 cut(s) 1647, 2039
AcuI CTGAAG 2 cut(s) 683, 1895
AcyI GRCGYC 4 cut(s) 23, 236, 249, 1481
AdeI CACNNNGTG 1 cut(s) 674
AfiI CCNNNNNNNGG 1 cut(s) 135
AflII CTTAAG 1 cut(s) 1153
AgsI TTSAA 9 cut(s) 160, 414, 751, 931, 1139, 1304, 1347, 1610, 2148
AjnI CCWGG 1 cut(s) 203
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
AloI GAACNNNNNNTCC 2 cut(s) 891, 923
AluBI AGCT 8 cut(s) 211, 435, 914, 1007, 1075, 1456, 1460, 1691
AluI AGCT 8 cut(s) 211, 435, 914, 1007, 1075, 1456, 1460, 1691
Alw26I GTCTC 4 cut(s) 13, 939, 1148, 1247
AlwI GGATC 5 cut(s) 752, 1395, 1408, 1427, 2018
Ama87I CYCGRG 2 cut(s) 230, 356
AoxI GGCC 6 cut(s) 30, 43, 126, 201, 1031, 1802
ApeKI GCWGC 4 cut(s) 325, 585, 609, 914
ApoI RAATTY 2 cut(s) 1647, 2039
Asp700I GAANNNNTTC 3 cut(s) 906, 1539, 1955
AspLEI GCGC 3 cut(s) 16, 355, 1774
AspS9I GGNCC 4 cut(s) 201, 528, 806, 1032
AsuC2I CCSGG 1 cut(s) 2075
AsuHPI GGTGA 6 cut(s) 31, 205, 346, 463, 562, 2120
AsuII TTCGAA 1 cut(s) 1057
AsuNHI GCTAGC 2 cut(s) 422, 1456
AvaI CYCGRG 2 cut(s) 230, 356
AvaII GGWCC 2 cut(s) 528, 806
BalI TGGCCA 2 cut(s) 32, 1804
BamHI GGATCC 1 cut(s) 1400
BanI GGYRCC 2 cut(s) 944, 1270
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BauI CACGAG 1 cut(s) 1186
BbvI GCAGC 4 cut(s) 312, 597, 621, 901
BccI CCATC 7 cut(s) 83, 482, 581, 984, 1056, 1430, 1509
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 205
BciVI GTATCC 3 cut(s) 1223, 1727, 1892
BcnI CCSGG 1 cut(s) 2075
BcoDI GTCTC 4 cut(s) 13, 939, 1148, 1247
BfaI CTAG 5 cut(s) 164, 423, 578, 1457, 1688
BfmI CTRYAG 3 cut(s) 600, 1788, 1887
BfoI RGCGCY 1 cut(s) 17
BfrI CTTAAG 1 cut(s) 1153
BfuI GTATCC 3 cut(s) 1223, 1727, 1892
BisI GCNGC 7 cut(s) 266, 326, 427, 586, 610, 915, 948
BlsI GCNGC 7 cut(s) 267, 327, 428, 587, 611, 916, 949
BmcAI AGTACT 3 cut(s) 1353, 1525, 1943
Bme1390I CCNGG 2 cut(s) 205, 2075
Bme18I GGWCC 2 cut(s) 528, 806
BmeT110I CYCGRG 2 cut(s) 230, 356
BmgT120I GGNCC 4 cut(s) 201, 528, 806, 1032
BmiI GGNNCC 5 cut(s) 194, 808, 946, 1272, 1402
BmrFI CCNGG 2 cut(s) 205, 2075
BmrI ACTGGG 1 cut(s) 1233
BmsI GCATC 5 cut(s) 340, 554, 706, 1901, 2073
BmtI GCTAGC 2 cut(s) 426, 1460
BmuI ACTGGG 1 cut(s) 1233
Bpu14I TTCGAA 1 cut(s) 1057
BpuEI CTTGAG 2 cut(s) 415, 866
BpuMI CCSGG 1 cut(s) 2075
BsaBI GATNNNNATC 3 cut(s) 1066, 1421, 2087
BsaHI GRCGYC 4 cut(s) 23, 236, 249, 1481
BsaI GGTCTC 1 cut(s) 1148
BsaJI CCNNGG 5 cut(s) 102, 216, 252, 810, 1775
BsaWI WCCGGW 2 cut(s) 331, 785
BsaXI ACNNNNNCTCC 2 cut(s) 891, 921
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse1I ACTGG 5 cut(s) 33, 531, 1034, 1159, 1239
Bse3DI GCAATG 1 cut(s) 1491
Bse8I GATNNNNATC 3 cut(s) 1066, 1421, 2087
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 5 cut(s) 102, 216, 252, 810, 1775
BseGI GGATG 7 cut(s) 75, 403, 545, 573, 649, 1295, 1520
BseJI GATNNNNATC 3 cut(s) 1066, 1421, 2087
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMI GCAATG 1 cut(s) 1491
BseMII CTCAG 3 cut(s) 71, 275, 1977
BseNI ACTGG 5 cut(s) 33, 531, 1034, 1159, 1239
BseRI GAGGAG 1 cut(s) 872
BseXI GCAGC 4 cut(s) 312, 597, 621, 901
BsgI GTGCAG 1 cut(s) 1458
Bsh1236I CGCG 2 cut(s) 118, 988
BshFI GGCC 6 cut(s) 32, 45, 128, 203, 1033, 1804
BshNI GGYRCC 2 cut(s) 944, 1270
BsiHKCI CYCGRG 2 cut(s) 230, 356
BsiSI CCGG 7 cut(s) 42, 303, 332, 345, 786, 1948, 2075
BslI CCNNNNNNNGG 1 cut(s) 135
BsmAI GTCTC 4 cut(s) 13, 939, 1148, 1247
BsmBI CGTCTC 1 cut(s) 13
BsmI GAATGC 3 cut(s) 588, 1095, 1191
BsnI GGCC 6 cut(s) 32, 45, 128, 203, 1033, 1804
Bso31I GGTCTC 1 cut(s) 1148
BsoBI CYCGRG 2 cut(s) 230, 356
Bsp119I TTCGAA 1 cut(s) 1057
Bsp143I GATC 6 cut(s) 376, 757, 826, 1400, 1432, 2023
BspACI CCGC 5 cut(s) 116, 135, 265, 426, 948
BspANI GGCC 6 cut(s) 32, 45, 128, 203, 1033, 1804
BspCNI CTCAG 3 cut(s) 72, 276, 1978
BspFNI CGCG 2 cut(s) 118, 988
BspHI TCATGA 1 cut(s) 2156
BspLI GGNNCC 5 cut(s) 194, 808, 946, 1272, 1402
BspMAI CTGCAG 1 cut(s) 1792
BspOI GCTAGC 2 cut(s) 426, 1460
BspPI GGATC 5 cut(s) 752, 1395, 1408, 1427, 2018
BspT104I TTCGAA 1 cut(s) 1057
BspT107I GGYRCC 2 cut(s) 944, 1270
BspTI CTTAAG 1 cut(s) 1153
BspTNI GGTCTC 1 cut(s) 1148
BsrDI GCAATG 1 cut(s) 1491
BsrI ACTGG 5 cut(s) 33, 531, 1034, 1159, 1239
BssECI CCNNGG 5 cut(s) 102, 216, 252, 810, 1775
BssMI GATC 6 cut(s) 376, 757, 826, 1400, 1432, 2023
BssNI GRCGYC 4 cut(s) 23, 236, 249, 1481
BssSI CACGAG 1 cut(s) 1186
BssT1I CCWWGG 1 cut(s) 810
Bst2BI CACGAG 1 cut(s) 1186
Bst2UI CCWGG 1 cut(s) 205
Bst4CI ACNGT 5 cut(s) 1366, 1390, 1592, 2048, 2108
Bst6I CTCTTC 5 cut(s) 62, 140, 1871, 1917, 2133
BstACI GRCGYC 4 cut(s) 23, 236, 249, 1481
BstAFI CTTAAG 1 cut(s) 1153
BstBI TTCGAA 1 cut(s) 1057
BstC8I GCNNGC 5 cut(s) 424, 1005, 1093, 1458, 1848
BstDEI CTNAG 5 cut(s) 80, 284, 1071, 1437, 1986
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 7 cut(s) 75, 403, 545, 573, 649, 1295, 1520
BstFNI CGCG 2 cut(s) 118, 988
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 3 cut(s) 16, 355, 1774
BstKTI GATC 6 cut(s) 379, 760, 829, 1403, 1435, 2026
BstMAI GTCTC 4 cut(s) 13, 939, 1148, 1247
BstMBI GATC 6 cut(s) 376, 757, 826, 1400, 1432, 2023
BstMWI GCNNNNNNNGC 6 cut(s) 432, 953, 1457, 1472, 1953, 1982
BstNI CCWGG 1 cut(s) 205
BstNSI RCATGY 1 cut(s) 1642
BstSCI CCNGG 2 cut(s) 203, 2073
BstSFI CTRYAG 3 cut(s) 600, 1788, 1887
BstUI CGCG 2 cut(s) 118, 988
BstV1I GCAGC 4 cut(s) 312, 597, 621, 901
BstX2I RGATCY 2 cut(s) 757, 1400
BstYI RGATCY 2 cut(s) 757, 1400
BsuI GTATCC 3 cut(s) 1223, 1727, 1892
BsuRI GGCC 6 cut(s) 32, 45, 128, 203, 1033, 1804
BtgI CCRYGG 1 cut(s) 252
BtgZI GCGATG 2 cut(s) 363, 1003
BtsCI GGATG 7 cut(s) 75, 403, 545, 573, 649, 1295, 1520
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 5 cut(s) 26, 465, 622, 1219, 1871
Cac8I GCNNGC 5 cut(s) 424, 1005, 1093, 1458, 1848
CciI TCATGA 1 cut(s) 2156
CfoI GCGC 3 cut(s) 16, 355, 1774
Cfr13I GGNCC 4 cut(s) 201, 528, 806, 1032
CseI GACGC 5 cut(s) 225, 257, 601, 850, 1470
CviAII CATG 4 cut(s) 1019, 1282, 1639, 2157
DdeI CTNAG 5 cut(s) 80, 284, 1071, 1437, 1986
DpnI GATC 6 cut(s) 378, 759, 828, 1402, 1434, 2025
DpnII GATC 6 cut(s) 376, 757, 826, 1400, 1432, 2023
DraIII CACNNNGTG 1 cut(s) 674
EaeI YGGCCR 3 cut(s) 30, 126, 1802
Eam1104I CTCTTC 5 cut(s) 62, 140, 1871, 1917, 2133
EarI CTCTTC 5 cut(s) 62, 140, 1871, 1917, 2133
Eco130I CCWWGG 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 1148
Eco32I GATATC 1 cut(s) 1068
Eco47I GGWCC 2 cut(s) 528, 806
Eco57I CTGAAG 2 cut(s) 683, 1895
Eco88I CYCGRG 2 cut(s) 230, 356
EcoO109I RGGNCCY 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 1647
EcoRII CCWGG 1 cut(s) 203
EcoRV GATATC 1 cut(s) 1068
EcoT14I CCWWGG 1 cut(s) 810
ErhI CCWWGG 1 cut(s) 810
Esp3I CGTCTC 1 cut(s) 13
FaeI CATG 4 cut(s) 1022, 1285, 1642, 2160
FatI CATG 4 cut(s) 1018, 1281, 1638, 2156
FauI CCCGC 1 cut(s) 128
FblI GTMKAC 2 cut(s) 108, 490
Fnu4HI GCNGC 7 cut(s) 266, 326, 427, 586, 610, 915, 948
FokI GGATG 7 cut(s) 62, 410, 532, 560, 636, 1282, 1527
Fsp4HI GCNGC 7 cut(s) 266, 326, 427, 586, 610, 915, 948
FspBI CTAG 5 cut(s) 164, 423, 578, 1457, 1688
GlaI GCGC 3 cut(s) 15, 354, 1773
GluI GCNGC 7 cut(s) 266, 326, 427, 586, 610, 915, 948
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 6 cut(s) 32, 45, 128, 203, 1033, 1804
HapII CCGG 7 cut(s) 42, 303, 332, 345, 786, 1948, 2075
HgaI GACGC 5 cut(s) 225, 257, 601, 850, 1470
HhaI GCGC 3 cut(s) 16, 355, 1774
Hin1I GRCGYC 4 cut(s) 23, 236, 249, 1481
Hin1II CATG 4 cut(s) 1022, 1285, 1642, 2160
Hin6I GCGC 3 cut(s) 14, 353, 1772
HinP1I GCGC 3 cut(s) 14, 353, 1772
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HindIII AAGCTT 1 cut(s) 433
HinfI GANTC 8 cut(s) 175, 445, 633, 703, 738, 1564, 1863, 2115
HpaII CCGG 7 cut(s) 42, 303, 332, 345, 786, 1948, 2075
HphI GGTGA 6 cut(s) 31, 205, 346, 463, 562, 2120
Hpy188I TCNGA 8 cut(s) 103, 197, 702, 757, 806, 883, 1263, 1534
Hpy99I CGWCG 2 cut(s) 241, 687
HpyAV CCTTC 9 cut(s) 125, 189, 192, 663, 937, 1045, 1189, 1244, 1717
HpyCH4III ACNGT 5 cut(s) 1366, 1390, 1592, 2048, 2108
HpyCH4IV ACGT 4 cut(s) 23, 363, 781, 1500
HpyF10VI GCNNNNNNNGC 6 cut(s) 432, 953, 1457, 1472, 1953, 1982
HpyF3I CTNAG 5 cut(s) 80, 284, 1071, 1437, 1986
HpySE526I ACGT 4 cut(s) 23, 363, 781, 1500
Hsp92I GRCGYC 4 cut(s) 23, 236, 249, 1481
Hsp92II CATG 4 cut(s) 1022, 1285, 1642, 2160
HspAI GCGC 3 cut(s) 14, 353, 1772
Kzo9I GATC 6 cut(s) 376, 757, 826, 1400, 1432, 2023
LmnI GCTCC 6 cut(s) 198, 347, 606, 874, 1012, 1696
Lsp1109I GCAGC 4 cut(s) 312, 597, 621, 901
LweI GCATC 5 cut(s) 340, 554, 706, 1901, 2073
MaeI CTAG 5 cut(s) 164, 423, 578, 1457, 1688
MaeII ACGT 4 cut(s) 23, 363, 781, 1500
MaeIII GTNAC 8 cut(s) 19, 24, 469, 666, 994, 1504, 1634, 2108
MalI GATC 6 cut(s) 378, 759, 828, 1402, 1434, 2025
MboI GATC 6 cut(s) 376, 757, 826, 1400, 1432, 2023
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 2 cut(s) 757, 1400
MlsI TGGCCA 2 cut(s) 32, 1804
MluNI TGGCCA 2 cut(s) 32, 1804
MlyI GAGTC 4 cut(s) 732, 1573, 1872, 2109
MmeI TCCRAC 2 cut(s) 1143, 1840
Mox20I TGGCCA 2 cut(s) 32, 1804
MroXI GAANNNNTTC 3 cut(s) 906, 1539, 1955
MscI TGGCCA 2 cut(s) 32, 1804
MseI TTAA 4 cut(s) 816, 1154, 1935, 1997
MslI CAYNNNNRTG 1 cut(s) 1513
Msp20I TGGCCA 2 cut(s) 32, 1804
MspCI CTTAAG 1 cut(s) 1153
MspI CCGG 7 cut(s) 42, 303, 332, 345, 786, 1948, 2075
MspR9I CCNGG 2 cut(s) 205, 2075
MunI CAATTG 1 cut(s) 951
Mva1269I GAATGC 3 cut(s) 588, 1095, 1191
MvaI CCWGG 1 cut(s) 205
MvnI CGCG 2 cut(s) 118, 988
MwoI GCNNNNNNNGC 6 cut(s) 432, 953, 1457, 1472, 1953, 1982
NciI CCSGG 1 cut(s) 2075
NdeII GATC 6 cut(s) 376, 757, 826, 1400, 1432, 2023
NheI GCTAGC 2 cut(s) 422, 1456
NlaIII CATG 4 cut(s) 1022, 1285, 1642, 2160
NlaIV GGNNCC 5 cut(s) 194, 808, 946, 1272, 1402
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 8 cut(s) 19, 24, 469, 666, 994, 1504, 1634, 2108
NspI RCATGY 1 cut(s) 1642
NspV TTCGAA 1 cut(s) 1057
PaeR7I CTCGAG 2 cut(s) 230, 356
PagI TCATGA 1 cut(s) 2156
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PctI GAATGC 3 cut(s) 588, 1095, 1191
PdmI GAANNNNTTC 3 cut(s) 906, 1539, 1955
PfeI GAWTC 4 cut(s) 175, 445, 633, 703
PkrI GCNGC 7 cut(s) 267, 327, 428, 587, 611, 916, 949
PleI GAGTC 4 cut(s) 732, 1572, 1871, 2109
PpsI GAGTC 4 cut(s) 732, 1572, 1871, 2109
PsiI TTATAA 1 cut(s) 1085
Psp1406I AACGTT 1 cut(s) 781
Psp6I CCWGG 1 cut(s) 203
PspGI CCWGG 1 cut(s) 203
PspN4I GGNNCC 5 cut(s) 194, 808, 946, 1272, 1402
PspPI GGNCC 4 cut(s) 201, 528, 806, 1032
PspXI VCTCGAGB 2 cut(s) 230, 356
PstI CTGCAG 1 cut(s) 1792
PsuI RGATCY 2 cut(s) 757, 1400
RseI CAYNNNNRTG 1 cut(s) 1513
SaqAI TTAA 4 cut(s) 816, 1154, 1935, 1997
SatI GCNGC 7 cut(s) 266, 326, 427, 586, 610, 915, 948
Sau3AI GATC 6 cut(s) 376, 757, 826, 1400, 1432, 2023
Sau96I GGNCC 4 cut(s) 201, 528, 806, 1032
ScaI AGTACT 3 cut(s) 1353, 1525, 1943
SchI GAGTC 4 cut(s) 732, 1573, 1872, 2109
ScrFI CCNGG 2 cut(s) 205, 2075
SfaNI GCATC 5 cut(s) 340, 554, 706, 1901, 2073
SfcI CTRYAG 3 cut(s) 600, 1788, 1887
Sfr274I CTCGAG 2 cut(s) 230, 356
SfuI TTCGAA 1 cut(s) 1057
SinI GGWCC 2 cut(s) 528, 806
SlaI CTCGAG 2 cut(s) 230, 356
SmiMI CAYNNNNRTG 1 cut(s) 1513
SmlI CTYRAG 5 cut(s) 230, 356, 430, 845, 1153
SmoI CTYRAG 5 cut(s) 230, 356, 430, 845, 1153
SsiI CCGC 5 cut(s) 116, 135, 265, 426, 948
SspMI CTAG 5 cut(s) 164, 423, 578, 1457, 1688
StyD4I CCNGG 2 cut(s) 203, 2073
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 5 cut(s) 1366, 1390, 1592, 2048, 2108
TaiI ACGT 4 cut(s) 26, 366, 784, 1503
TaqI TCGA 5 cut(s) 231, 357, 685, 910, 1057
TatI WGTACW 5 cut(s) 1129, 1351, 1523, 1660, 1941
TauI GCSGC 3 cut(s) 268, 429, 950
TfiI GAWTC 4 cut(s) 175, 445, 633, 703
Tru1I TTAA 4 cut(s) 816, 1154, 1935, 1997
Tru9I TTAA 4 cut(s) 816, 1154, 1935, 1997
TscAI CASTG 5 cut(s) 33, 465, 622, 1219, 1878
TseFI GTSAC 8 cut(s) 19, 24, 469, 666, 994, 1504, 1634, 2108
TseI GCWGC 4 cut(s) 325, 585, 609, 914
Tsp45I GTSAC 8 cut(s) 19, 24, 469, 666, 994, 1504, 1634, 2108
TspGWI ACGGA 1 cut(s) 1393
TspRI CASTG 5 cut(s) 33, 465, 622, 1219, 1878
Vha464I CTTAAG 1 cut(s) 1153
VpaK11BI GGWCC 2 cut(s) 528, 806
XapI RAATTY 2 cut(s) 1647, 2039
XbaI TCTAGA 1 cut(s) 163
XceI RCATGY 1 cut(s) 1642
XhoI CTCGAG 2 cut(s) 230, 356
XmiI GTMKAC 2 cut(s) 108, 490
XmnI GAANNNNTTC 3 cut(s) 906, 1539, 1955
XspI CTAG 5 cut(s) 164, 423, 578, 1457, 1688
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 3 cut(s) 1353, 1525, 1943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.